Rotational flow tube type microalgae sorting device

Abstract

The utility model relates to a cyclone tube type microalgae sorting device which comprises a water inlet and a fluid distribution unit, one end of the water inlet is connected with the fluid distribution unit, and the fluid distribution unit comprises an inner ring flow channel layer, an outer ring flow channel layer and a controllable water outlet. The number of the controllable water outlets in the fluid distribution unit in the microalgae sorting device is at least two, different flows are provided for the microalgae solution to be sorted, the microalgae solution with the optimal high concentration is sorted out, the number of the water outlets is controlled according to the characteristics of the microalgae species, and the microalgae species in different growth stages can be sorted. Under the condition that the number of water outlets is different during sorting, sorting indexes are accurately compared, analyzed and summarized, efficient sorting of microalgae is achieved, time and labor are saved, the operation cost is low, and good application prospects are provided.

Description

Technical Field

[0001] The utility model relates to the technical field of environmental water conservancy, and particularly relates to a cyclone tube microalgae sorting device. Background Technique

[0002] There are various types of microalgae in nature. The prerequisite for developing new microalgae varieties and obtaining excellent algal strains is to sort high-quality algae from nature. There are significant differences among different microalgae, which are reflected in their morphology, growth environment and applications. The sorted high-quality algae have a very wide range of application fields. Microalgae can efficiently fix carbon dioxide through photosynthesis, which helps to reduce greenhouse gases in the atmosphere. In addition, they play the role of basic producers in the water ecosystem, providing food and oxygen for other aquatic organisms. Therefore, the evaluation and screening of excellent microalgae strains are fundamental tasks for large-scale cultivation of microalgae and the development of microalgae products. Since different algal strains have their own characteristics, such as different morphological characteristics and different growth environments, it is difficult to sort out different high-quality algae, and at the same time, people's requirements for the screening of high-quality algal strains are also difficult to be met.

[0003] At the same time, microalgae also widely exist in rivers, lakes and water source reservoirs, and also have some adverse effects on the environment and human activities. When microalgae over-reproduce in water bodies, harmful algal blooms (HABs) will be formed. These algal blooms not only destroy the balance of the aquatic ecosystem, but even endanger human health seriously. During the large-scale cultivation of microalgae, it may be affected by pollution organisms such as zooplankton. These organisms will compete with microalgae for nutrients, affecting the growth and yield of microalgae. Research has found that these phenomena are caused by some algal strains. Therefore, in the process of environmental monitoring of drinking water sources rich in algae and the development of biological resource safety utilization technology, there is an urgent need to invent a method and application based on an algae sorting device.

[0004] Existing traditional microalgae sorting methods include micro-pipette sorting method, water-drop sorting method, plate coating method, antibiotic sorting method, dilution sorting method, as well as solvent extraction, weight determination method, etc. These methods have low sorting efficiency, difficult operation, time-consuming and laborious, and require high professional skills for operators. It can be seen that the prior art cannot well provide the best screening for several microalgae of the same kind. Therefore, there is an urgent need to develop detection and sorting devices and sorting methods that can be used for sorting microalgae at different growth stages. Content of the Utility Model

[0005] The utility model provides a cyclone tube sorting device for microalgae of different algal strains, which can realize the sorting of water microalgae, is simple and convenient to operate, and has a high sorting rate, so as to overcome at least one technical problem existing in the prior art.

[0006] The utility model provides a cyclone tube sorting device for microalgae strains, and adopts the following technical solutions:

[0007] The cyclone tube microalgae sorting device includes a water inlet, a microfluid pump, an inner circulation channel layer, an outer circulation channel layer, a controllable water outlet, and an infusion hose; one end of the water inlet is connected to the microfluid pump through the infusion hose, and the other end is connected to the inner annular flow channel layer through the infusion hose. One end of the microfluid pump is connected to the water inlet through the infusion hose, and the other end is placed into a beaker containing 200 ml of cultured microalgae solution through the infusion hose; one end of the inner circulation channel layer is connected to the inlet pipe of the inner annular flow channel layer of the water inlet, and the other end is communicated with the outer circulation channel layer. One end of the outer circulation channel layer is communicated with the inner circulation channel layer, and the other end is connected to the first water outlet, the second water outlet, the third water outlet, and the fourth water outlet. The water outlet is connected to the infusion hose. The water inlet, the first water outlet, the second water outlet, the third water outlet, and the fourth water outlet must be placed on the same horizontal plane, and the inclination angle is between 0 and 2 degrees.

[0008] In the cyclone tube microalgae sorting device, the internal flow channel layer of the sorting device adopts an annular flow channel layer, namely an inner circulation channel layer and an outer circulation channel layer.

[0009] In the cyclone tube microalgae sorting device, the water inlet is axially perpendicular to the fluid distribution unit.

[0010] In the cyclone tube microalgae sorting device, the number of the controllable water outlets can be set to 4, 6, or 10.

[0011] In the cyclone tube microalgae sorting device, the included angle between the controllable water outlet and the outer circulation channel layer can be designed to be 45 degrees or 60 degrees.

[0012] In the cyclone tube microalgae sorting device, the water inlet is connected to the infusion hose, and one end is placed into a beaker containing 200 ml of cultured microalgae strain solution.

[0013] In the cyclone tube microalgae sorting device, the sorting device is fixed on a plane at the same height as the microfluid pump, and the height difference is between 0 mm and 10 mm.

[0014] In the cyclone tube microalgae sorting device, the sorting device is fixed on a horizontal plane, and the inclination angle is between 0 and 2 degrees.

[0015] In the cyclone tube microalgae sorting device, the connection method of the infusion hose is a sleeve connection method, and the connection is tight when using the sleeve connection.

[0016] Preferably, a cyclone tube microalgae sorting device, wherein the first water outlet, the second water outlet, the third water outlet and the fourth water outlet are connected to an infusion hose, and the length of the infusion hose is 18 mm to 20 mm.

[0017] Preferably, a cyclone tube microalgae sorting device, the infusion hose, in the sorting device experiment, the diameter of the infusion hose can be 4 mm to 6 mm. The controllable water outlet is docked with the outer circulation channel layer, the outer circulation channel layer is connected to the inner circulation channel layer, and the inner circulation channel layer and the outer circulation channel layer are microchannel structures. Microalgae solutions at different growth stages have different sizes of algal species. When sorting, the inner circulation channel layer and the outer circulation channel layer will select the size of the microchannel structure that is beneficial to efficient sorting according to the microalgae solutions at different growth stages. When the size of the microchannel structure does not match the selection of algal species at different growth stages during sorting, it will block the sorting device or the sorting effect will be poor. Selecting the size of the microchannel structure that matches the microalgae solutions at different growth stages can effectively improve the sorting efficiency and facilitate the sorting of microalgae solutions.

[0018] In summary, the advantages of the present utility model are as follows:

[0019] (1) Reasonable structural design: In the cyclone tube microalgae sorting device, the flow channel layer of the sorting device is divided into an inner circulation channel layer and an outer shape flow channel layer. The inner circulation channel layer is a circular structure, and the inner circular flow channel structure helps with flow distribution. The outer circulation channel layer adopts a circular structure, and the outer circular flow channel structure helps with the inertial swirl separation of algal species. The circular flow channel structure helps with the flow of microalgae algal species. Therefore, the sorting of microalgae solutions is not easily blocked. The outlet of the sorting device is a controllable water outlet. Different algal species have different sorting effects when the number of controllable water outlets is different, expanding the sorting space dimension of the traditional algae sorting device. It is suitable for carrying and has the characteristics of simple structure, convenient processing, small floor area, high sorting rate, not easily blocked, stable operation, low cost, and convenient operation and maintenance management.

[0020] (2) Simple functional operation: In the cyclone tube microalgae sorting device, the sorting device is made of a polymer light-transmitting material with a light transmittance greater than 70%. During sorting, the flow rate and flow direction of different algal species solutions in the sorting device can be clearly observed through a microscope, and the best sorting state can be analyzed through the sorting results. Description of the Drawings

[0021] Figure 1 It is a top view structural schematic diagram of the microalgae sorting device of the present utility model.

[0022] Figure 2 It is a top view structural schematic diagram of the present utility model with 6 controllable water outlets.

[0023] Figure 3 It is a front view of the water inlet of the microalgae sorting device of the present utility model.

[0024] Figure 4 It is the flow chart of the microalgae sorting device of the present utility model.

[0025] In the figure: 1 - water inlet, 2 - inner circulation channel layer inlet pipe, 3 - inner circulation channel layer water inlet, 4 - inner circulation channel layer, 5 - inner circulation channel layer water outlet, 6 - outer circulation channel layer inlet pipe, 7 - outer circulation channel layer water inlet, 8 - outer circulation channel layer, 9 - outer circulation channel layer water outlet, 10 - controllable water outlet. Specific implementation manner

[0026] In order to enable the personnel in the technical field to better understand the solution of this application, the following will clearly and completely describe the technical solutions in the embodiments of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0027] Embodiment 1

[0028] This embodiment proposes a microalgae cyclone tube sorting device for Chlorella, referring to Figure 1 There are 4 controllable water outlets, Figure 1 It is the top view schematic diagram of the microalgae sorting device of the present utility model.

[0029] The described one is a Chlorella cyclone tube microalgae sorting device, as Figure 1 shown, its structure includes a water inlet 1, an inner circulation channel layer 4, an outer circulation channel layer 8 and a controllable water outlet 10. The controllable water outlet includes a first water outlet, a second water outlet, a third water outlet and a fourth water outlet. When conducting a sorting experiment, the water inlet 1 and the controllable water outlet 10 of the sorting device are horizontally placed on the same horizontal plane with an inclination error of 0 to 2 degrees to reduce the influence of the self-weight of water flow.

[0030] For the described Chlorella cyclone tube microalgae sorting device, through experiments, it is obtained that when sorting Chlorella, there are 4 controllable water outlets of the sorting device, and the sorting effect of Chlorella is the best.

[0031] Measure 200 ml of the microalgae solution and place it in a beaker, and continuously stir it evenly with a glass. Conduct an absorbance test on 200 ml of the microalgae. When conducting the absorbance test, the presence of bubbles cannot affect the test results.

[0032] Place the infusion hose in 200 ml of microalgae solution. Open the inlet valve. Under the thrust of the microfluidic pump, the microalgae flow into the inner circulation channel layer inlet pipe 2 through the water inlet 1. The inner circulation channel layer inlet pipe 2 is connected to the inner circulation channel layer inlet 3 and flows into the inner circulation channel layer 4. The inner circulation channel layer 4 is connected to the inner annular flow channel layer outlet 5 and flows into the outer circulation channel layer inlet pipe 6. The outer circulation channel layer inlet pipe 6 is connected to the outer circulation channel layer inlet 7 and flows into the outer circulation channel layer 8 through the outer circulation channel layer inlet 7. The outer circulation channel layer 8 is connected to the outer circulation channel layer outlet 9 and flows into the controllable outlet 10. Then, it flows out through the first outlet, the second outlet, the third outlet, the fourth outlet respectively, and the connected infusion hose is placed in an empty beaker.

[0033] One end of the first outlet of the sorting device communicates with the outer circulation channel layer and the other end is connected to the infusion hose. One end of the second outlet communicates with the outer circulation channel layer and the other end is connected to the infusion hose. One end of the third outlet communicates with the outer circulation channel layer and the other end is connected to the infusion hose. One end of the fourth outlet communicates with the outer circulation channel layer and the other end is connected to the infusion hose.

[0034] The length of the infusion hose connecting the water inlet of the device to the microfluidic pump is 180 mm - 200 mm. The connection of the infusion hose is tight and there should be no liquid leakage or air leakage, as serious liquid leakage or air leakage will seriously affect the sorting result.

[0035] The first outlet, the second outlet, the third outlet and the fourth outlet of the sorting device are connected to the infusion hose. A beaker is placed below the infusion hose. The length of the outlet connecting the infusion hose is 18 mm - 20 mm to reduce the influence of bubbles in the infusion hose during sorting.

[0036] The fluid delivery device is a microfluidic pump, which can adjust the flow rate of the microalgae solution during the sorting experiment and control different flow velocities.

[0037] For the infusion hose, during the sorting device experiment, the diameter of the infusion hose is 3 mm - 6 mm. In the sorting experiment, a disposable infusion hose is used to avoid the appearance of bubbles and other microalgae solutions. Bubbles in the infusion pipe will block the sorting of microalgae. The more bubbles there are, the slower the flow rate of the microalgae solution during sorting, and the greater the impact on the sorting experiment. During the sorting experiment, the infusion hose should be kept unblocked, and check whether there is any damage to the infusion pipe to prevent the influence of liquid leakage and air leakage of the infusion hose on the sorting experiment.

[0038] Embodiment 2

[0039] This embodiment presents a swirling tube sorting device for a kind of microalgae of Pediastrum, referring to Figure 2 There are 6 controllable outlets. Figure 2 This is the front view structural schematic diagram of the microalgae sorting device of the present utility model.

[0040] The described Pediastrum cyclone tube algae sorting device, as Figure 2 shown, its structure includes a water inlet 1, an inner circulation channel layer 4, an outer circulation channel layer 8, and a controllable water outlet 10. The controllable water outlet includes a first water outlet, a second water outlet, a third water outlet, a fourth water outlet, a fifth water outlet, and a sixth water outlet. When conducting a sorting experiment, the water inlet 1 and the water outlet 10 of the sorting device are horizontally placed on the same horizontal plane with an inclination error of 0 to 2 degrees.

[0041] For the described Pediastrum cyclone tube algae sorting device, through experiments, it is obtained that when sorting Pediastrum, the best sorting effect of Pediastrum is achieved when the water outlet of the sorting device is 6.

[0042] Measure 200 ml of the microalgae solution and place it in a beaker, stir it evenly with a glass, measure the absorbance of the 200 ml of microalgae, and connect the microfluidic pump and the sorting device with an infusion hose.

[0043] Place the infusion hose in the 200 ml of microalgae solution, open the liquid inlet valve, and the microalgae flows into the inner circulation channel layer inlet pipe 2 through the water inlet 1 under the thrust of the microfluidic pump. The inner circulation channel layer inlet pipe 2 is connected to the inner circulation channel layer water inlet 3, flows into the inner circulation channel layer 4, the inner circulation channel layer 4 is connected to the inner annular flow channel layer water outlet 5 and flows into the outer circulation channel layer inlet pipe 6. The outer circulation channel layer inlet pipe 6 is connected to the outer circulation channel layer water inlet 7, flows into the outer circulation channel layer 8 through the outer circulation channel layer water inlet 7, the outer circulation channel layer 8 is connected to the outer circulation channel layer water outlet 9, and flows into the controllable water outlet 10 through the outer circulation channel layer water outlet 9 and is respectively connected to the infusion pipes at the first water outlet, the second water outlet, the third water outlet, the fourth water outlet, the fifth water outlet, and the sixth water outlet and placed in an empty beaker.

[0044] Start the microfluidic pump and time it for 20 minutes. After the sorting is completed, measure the microalgae solution at the first water outlet, the second water outlet, the third water outlet, the fourth water outlet, the fifth water outlet, and the sixth water outlet, and conduct an absorbance test.

[0045] The sorting device includes an inner circulation channel layer and an outer circulation channel layer, including a sorting device water inlet, a first water outlet, a second water outlet, a third water outlet, a fourth water outlet, a fifth water outlet, and a sixth water outlet.

[0046] One end of the sorting device water inlet is communicated with the inner circulation channel layer and the other end is connected to the peristaltic pump.

[0047] One end of the first water outlet of the sorting device communicates with the outer circulation channel layer, and the other end is connected to an infusion hose. One end of the second water outlet communicates with the outer circulation channel layer, and the other end is connected to an infusion hose. One end of the third water outlet communicates with the outer circulation channel layer, and the other end is connected to an infusion hose. One end of the fourth water outlet communicates with the outer circulation channel layer, and the other end is connected to an infusion hose. One end of the fifth water outlet communicates with the outer circulation channel layer, and the other end is connected to an infusion hose. One end of the sixth water outlet communicates with the outer flow channel layer, and the other end is connected to an infusion hose.

[0048] The length of the infusion hose connecting the device water inlet and the microfluidic pump is 180 mm to 200 mm, and the connection of the infusion hose is tight without liquid leakage or air leakage.

[0049] The first water outlet, the second water outlet, the third water outlet, the fourth water outlet, the fifth water outlet and the sixth water outlet of the sorting device are connected to infusion hoses. The length of the infusion hoses is 18 mm to 20 m, and beakers are placed below the infusion hoses.

[0050] Through the above embodiments of the present application, a microalgae sorting method is proposed. Different numbers of water outlets of the sorting device can sort different algal species. After sorting, the concentrations of the microalgae solutions at each water outlet are different, which can achieve the result of sorting and sampling. Innovative solutions are proposed for problems such as long labor time and low sorting accuracy. In particular, it can solve the problems caused by easy blockage, long sorting time, low sorting efficiency and other problems in the traditional method. Therefore, the microalgae sorting device of the present utility model helps to improve the microalgae sorting accuracy and efficiency, and provides a technical method for water quality safety assessment.

Claims

1. A cyclone tube microalgae sorting device, characterized in that It includes a water inlet, a fluid distribution unit, and an infusion hose. The fluid distribution unit includes an inner circulation channel layer, an outer circulation channel layer, and a controllable water outlet; the water inlet is tangentially connected to the inner circulation channel layer and the water inlet pipe, the outer circulation channel layer and the inner circulation channel layer are connected in a staggered manner by pipes of different heights, the flow channel layers of the inner circulation channel layer and the outer circulation channel layer are of a micro-channel structure, the outer circulation channel layer is connected to the controllable water outlet, and after the microalgae enter the fluid distribution unit through the water inlet, they flow out through the controllable water outlet via the annular flow channel layer, and the microalgae at different growth stages are sorted out.

2. The swirl tube type microalgae sorting device according to claim 1, characterized in that: The water inlet is axially perpendicular to the inner annular flow channel layer.

3. The swirl tube type microalgae sorting device according to claim 1, characterized in that: For the fluid distribution unit, the inner circulation channel layer is of an annular structure, and the inner annular flow channel structure helps with flow distribution. The outer circulation channel layer adopts an annular structure, and the outer circulation channel structure helps with inertial swirl separation.

4. The swirl tube type microalgae sorting device according to claim 1, characterized in that: For the fluid distribution unit, the diameter of the inner circulation channel layer is at least 0.2 mm, and the diameter of the outer circulation channel layer is at least 0.4 mm.

5. As claimed in 1 the swirl tube type microalgae sorting device described above, characterized in that: For the fluid distribution unit, the inner circulation channel layer and the outer circulation channel layer are longitudinally parallel and in the same plane, but not limited to being in the same plane.

6. The swirl tube type microalgae sorting device according to claim 1, wherein: The water outlet angle of the controllable water outlet is at least 30 degrees, and the number of water outlets is at least two.

7. The swirl tube type microalgae sorting device according to claim 1, characterized in that: The fluid distribution unit is made of a polymer light-transmitting material with a light transmittance greater than 70%.

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