Carbon dioxide incubator

By setting up a water jacket and circulating water pipe in the incubator, combined with a chiller and a centrifugal fan, the problems of refrigeration vibration and temperature unevenness of the existing incubator are solved, and stable temperature control and air flow are achieved, which is suitable for cell culture.

CN223150568UActive Publication Date: 2025-07-25SHANGHAI BOLU BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202421534377.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-01
Publication Date
2025-07-25
Estimated Expiration
2034-07-01

AI Technical Summary

Technical Problem

The cooling method of existing incubators will cause vibration to affect cell adherence culture, or the temperature uniformity is poor, making it impossible to keep the temperature consistent in different locations in the incubator.

Method used

The circulating water pipe is arranged in a water jacket, connected to the chiller, and temperature control is carried out by cooling the chiller, combining the centrifugal fan and the air duct to form an air circulation to maintain the temperature stability.

Benefits of technology

It realizes vibration-free temperature control, fast temperature recovery, good stability, uniform air flow, and suitable for cell culture.

✦ Generated by Eureka AI based on patent content.

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  • Figure CN223150568U_ABST
    Figure CN223150568U_ABST
Patent Text Reader

Abstract

The utility model relates to the technical field of cell culture equipment, and discloses a carbon dioxide incubator which comprises an incubator body and a control unit, an outer door is arranged on the front side of the incubator body, a water jacket is arranged on the left wall and the right wall and / or the rear wall and / or the bottom and / or the top of the inner side of the incubator body, and the water jacket is of a flat plate sealing structure and is fixedly arranged on the inner side of the incubator body. A circulating water pipe is arranged in the water jacket, the circulating water pipe is filled with liquid water, a water inlet end and a water outlet end of the circulating water pipe extend out of the box body and are connected with a cooling-water machine, and after the cooling-water machine cools the liquid water in the water jacket through the circulating water pipe, the water jacket cools the interior of the box body through radiation. The water jacket is installed in the box body, the circulating water pipe is arranged in the water jacket, the water jacket is connected with the cooling-water machine, liquid water in the water jacket is cooled through the cooling-water machine, and then the box body is cooled through radiation.
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Description

Technical Field

[0001] The utility model relates to the technical field of microbial culture equipment, in particular to a carbon dioxide incubator. Background Art

[0002] For a cell incubator, it is necessary to ensure the stability of the temperature and carbon dioxide concentration inside the incubator. In the prior art, the incubator is heated by an electric heating method to raise the temperature when the temperature inside the incubator is relatively low. When the temperature inside the incubator is relatively high, refrigeration is required.

[0003] There are mainly two ways of refrigeration for existing incubators. One is compression refrigeration, where a compressor is installed on the incubator body. The refrigeration effect is good, but the vibration of the compressor directly affects the cell adhesion in the culture dish. The other is semiconductor refrigeration. Due to the small area of semiconductor refrigeration and large local temperature drop, the temperature uniformity inside the incubator is poor, and it is impossible to maintain the same or nearly the same culture temperature for cells at different positions inside the incubator. Summary of the Utility Model

[0004] The purpose of the utility model is to solve the above problems, and provide a carbon dioxide incubator. By installing a water jacket inside the box body, arranging a circulating water pipe inside the water jacket, connecting a chiller, after the chiller cools the liquid water inside the water jacket, the box body is cooled by radiation.

[0005] The technical solution adopted by the utility model is as follows:

[0006] A carbon dioxide incubator, characterized in that it includes a box body and a control unit. An outer door is provided on the front side of the box body. A water jacket is provided on the left and right walls and / or the rear wall and / or the bottom and / or the top inside the box body. The water jacket is a flat sealed structure and is fixedly arranged inside the box body. A circulating water pipe is arranged inside the water jacket, and the outside of the circulating water pipe is filled with liquid water. The inlet and outlet ends of the circulating water pipe extend outside the box body and are connected to a chiller. After the chiller cools the liquid water inside the water jacket through the circulating water pipe, the water jacket cools the inside of the box body by radiation.

[0007] Further, the water jacket is provided on the left and right walls, the back wall, the bottom and the top inside the box body, and the inside of the water jacket is connected and filled with liquid water.

[0008] Further, the circulating water pipe is arranged in a meandering manner inside the upper half of the water jacket.

[0009] Further, a carbon dioxide sensor is arranged inside the box body. A carbon dioxide inlet pipe is also arranged inside the box body. The carbon dioxide inlet pipe is connected to a carbon dioxide generator outside the box body. An intake electromagnetic valve is arranged on the carbon dioxide inlet pipe. The control unit controls the intake electromagnetic valve according to the carbon dioxide concentration inside the box body.

[0010] Furthermore, heating pipes are arranged on the outer side of the lower half part and below the bottom of the water jacket, and the heating pipes are arranged in a U-shaped circuitous layout.

[0011] Furthermore, a centrifugal fan is arranged at the top inside the box body. On the side wall on one side of the air outlet of the centrifugal fan, an air duct is arranged. The air outlet is connected to the upper end of the air duct, and the lower end opening of the air duct is located at the bottom position of the box body. After the air blown out by the centrifugal fan flows from the upper end to the lower end of the air duct, it is blown out from the lower end opening and flows through the inside of the box body to the air inlet of the centrifugal fan.

[0012] Furthermore, a high-efficiency filter is arranged at the air inlet of the centrifugal fan.

[0013] Furthermore, a plurality of shelves are arranged inside the box body, and array holes are formed on the shelves.

[0014] Furthermore, the outside of the box body is covered with a heat-insulating layer. A temperature sensor is arranged inside the box body, and the temperature sensor is connected to a control unit. A warm water tray is also arranged at the bottom of the box body.

[0015] Furthermore, an observation door is also arranged on the front side of the box body. The observation door is arranged inside the outer door. The observation door is of a double-layer glass structure, and an electric heating wire is arranged inside. The control unit controls the heating wire to work.

[0016] The beneficial effects of the present utility model are as follows:

[0017] (1) The chiller is independent of the incubator and will not generate vibration to affect the adherent culture of cells in the incubator;

[0018] (2) The water jacket has a wide covering area, fast temperature recovery and stable temperature;

[0019] (3) A circulating air is formed inside the box body through the centrifugal fan and the air duct to keep the air flow inside the box body. Description of the Drawings

[0020] Att Figure 1 is a schematic three-dimensional structure diagram of the incubator box body of the present utility model;

[0021] Att Figure 2 is a schematic diagram of the incubator connected to the chiller;

[0022] Att Figure 3 is the front view of the incubator;

[0023] Att Figure 4 is the C-C cross-sectional view of Att Figure 3 ;

[0024] Att Figure 5 is Att Figure 4E-E sectional view;

[0025] Appended Figure 6 is the top view of the incubator (without cold water pipe connected).

[0026] The reference numerals in the appended drawings are respectively:

[0027] 1. Box body; 2. Insulation layer;

[0028] 3. Outer door; 4. Observation door;

[0029] 5. Inner container; 6. Water jacket;

[0030] 7. Circulation water pipe; 8. Heating pipe;

[0031] 9. Air duct; 10. High-efficiency filter;

[0032] 11. Centrifugal fan; 12. Humidification water tray;

[0033] 13. Carbon dioxide sensor; 14. Temperature sensor;

[0034] 15. Electrical control box; 16. Shelf;

[0035] 17. Touch screen; 18. Cold water inlet;

[0036] 19. Cold water outlet; 20. Carbon dioxide inlet pipe;

[0037] 21. Intake solenoid valve. Specific embodiments

[0038] The following will make a detailed description of the specific embodiments of a carbon dioxide incubator of the present utility model with reference to the appended drawings.

[0039] Refer to the appended Figure 1 、 2 In the carbon dioxide incubator of the present utility model, the inside of the box body 1 is used to place culture dishes for microbial culture, and its outside is covered with an insulation layer 2 to keep a suitable temperature and humidity inside. The front side is an outer door 3, and an observation door 4 is also arranged inside the outer door 3. The observation door 4 is a double-glass structure, and an electric heating wire is arranged inside. The heating wire is uniformly controlled by the control unit of the carbon dioxide incubator to heat the observation door 4. The double-glass structure of the observation door 4 can reduce the heat exchange between the inside of the box body 1 and the outside. After the heating wire heats the observation door 4, it prevents the high-temperature and high-humidity air inside the box body 1 from condensing on the inner side of the observation door 4, affecting the observation line of sight of the glass door.

[0040] A chiller is arranged outside the incubator and is connected to the inside of the box body 1 through a pipeline to control the temperature reduction of the box body 1. The specific structure and process will be introduced later.

[0041] Refer to the appendedFigure 3 , 4 5, 6. An electric control box 15 is provided above the box body 1, serving as a control unit for various electrical components of the box body 1. Inside the inner side of the box body 1, a water jacket 6 is provided. The water jacket 6 is of a flat structure, with a stainless-steel inner liner 5 inside. Except for one side of the outer door 3, the other sides are integrated to semi-surround the box body 1. Liquid water is sealed inside the inner liner 5 of the water jacket 6. A circulating water pipe 7 is provided inside the water jacket 6. The inlet and outlet ends of the circulating water pipe 7 extend outside the box body 1 and are connected to a chiller. After the chiller cools the cooling water through the circulating water pipe 7 and supplies it to the liquid water inside the water jacket 6, the water jacket 6 cools the inside of the box body 1 through radiation.

[0042] The outer side of the water jacket 6 is a heat-insulating layer 2, which completely covers the outside of the box body 1 to keep a stable temperature and humidity environment inside the box body 1. The water jacket 6 can also be changed in position according to the need for temperature control of the box body 1, for example, it can be set on only one or more of the side, bottom, back, and top surfaces.

[0043] The circulating water pipe 7 inside the water jacket 6 is used to cool the liquid water inside the water jacket 6. According to the fluid principle, the circulating water pipe 7 only needs to be set in the upper half of the water jacket 6 and is arranged in a meandering manner. When the chiller circulates cold water in the circulating water pipe 7, the water in the upper half of the water jacket 6 exchanges heat with the cold water, and the liquid water inside the water jacket 6 forms a convective conduction, quickly making the temperature of the liquid water throughout the water jacket 6 consistent. The shell of the water jacket 6 is made of stainless steel, and it can exchange heat with the high-humidity air inside the box body 1 in a radiative conduction manner. Due to the large area setting of the water jacket 6, the temperature inside the box body 1 can reach the preset constant value in a short time.

[0044] The cold water inlet 18 and the cold water outlet 19 of the circulating water pipe 7 are set at the rear side of the box body 1, which does not affect the operation of the box body 1. The inlet pipe is located below and the outlet pipe is located above, making the temperature of the lower water jacket 6 lower, which is more conducive to the formation of convection.

[0045] A heating pipe 8 is provided on the outer side of the lower half of the water jacket 6 and below the bottom. The heating pipe 8 is arranged in a meandering U shape. The heating pipe 8 is controlled by the control unit to heat the water jacket 6. The heating pipe 8 is set at the lower half position of the water jacket 6, and it quickly heats the liquid water inside the water jacket 6 through the convection of water to reach the set temperature.

[0046] A carbon dioxide sensor 13 is provided inside the box body 1. A carbon dioxide inlet pipe 20 is also provided inside the box body 1. The carbon dioxide inlet pipe 20 is connected to a carbon dioxide generator outside the box body 1 (not shown in the figure). An intake solenoid valve 21 is provided on the carbon dioxide inlet pipe 20, and the control unit controls the intake solenoid valve 21 according to the concentration of carbon dioxide inside the box body 1.

[0047] A temperature sensor 14 is also arranged inside the box body 1. The control unit adjusts the chiller or the heating pipe 8 to heat or cool the water jacket 6 according to the feedback of the temperature sensor 14, so as to keep the box body 1 at a constant temperature. A humidifying water tray 12 is also arranged below the box body 1, which is used to adjust the air humidity inside the box body 1 to keep a relatively high humidity during the microorganism culture process.

[0048] A centrifugal fan 11 is arranged at the top inside the box body 1. On the side wall on one side of the air outlet of the centrifugal fan 11, an air duct 9 is arranged. The air outlet is connected to the upper end of the air duct 9, and the lower end opening of the air duct 9 is located at the bottom of the box body 1. The air blown out by the centrifugal fan 11 flows from the upper end to the lower end of the air duct 9 and then is blown out from the lower end opening, and flows through the inside of the box body 1 to the air inlet of the centrifugal fan 11. A high-efficiency filter 10 is arranged at the air inlet of the centrifugal fan 11. The arrow direction in the figure is the way of air flow. The air flow makes the temperature inside the box body 1 quickly consistent. In addition, it is also a requirement during the microorganism culture process, so that the gas generated by the growth of microorganisms can be quickly diffused, which is beneficial to the accurate sensing of the carbon dioxide sensor 13. The air flow also helps the water in the humidifying water tray 12 to quickly evaporate, so as to keep a high humidity inside the box body 1.

[0049] A plurality of shelves 16 are arranged inside the box body 1. The shelves 16 arranged in layers can place multiple culture dishes. Array holes are opened on the shelves 16, which is beneficial to the air flow when the centrifugal fan 11 works.

[0050] Rollers are arranged below the whole incubator to facilitate the movement of the equipment. A touch screen 17 is also arranged on the top of the incubator, and the incubator is set through the system interface.

[0051] The above is only the preferred embodiment of the present utility model. It should be pointed out that for those of ordinary skill in the art in this technical field, without departing from the principle of the present utility model, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present utility model.

Claims

1. A carbon dioxide incubator, characterized in that: It includes a box body and a control unit. An outer door is provided on the front side of the box body. A water jacket is provided on the left and right walls and / or the rear wall and / or the bottom and / or the top on the inner side of the box body. The water jacket is a flat sealed structure and is fixedly arranged on the inner side of the box body. A circulating water pipe is arranged inside the water jacket, and the outside of the circulating water pipe is filled with liquid water. The inlet and outlet ends of the circulating water pipe extend out of the box body and are connected to a chiller. After the chiller cools the liquid water in the water jacket through the circulating water pipe, the water jacket cools the inside of the box body by radiation.

2. The carbon dioxide incubator according to claim 1, wherein: The water jacket is arranged on the left and right walls, the back wall, the bottom and the top on the inner side of the box body, and the inside of the water jacket is communicated and filled with liquid water.

3. The carbon dioxide incubator according to claim 2, characterized in that: The circulating water pipe is arranged in a circuitous manner inside the upper half of the water jacket.

4. The carbon dioxide incubator according to claim 2, wherein: A carbon dioxide sensor is arranged inside the box body. A carbon dioxide inlet pipe is also arranged inside the box body. The carbon dioxide inlet pipe is connected to a carbon dioxide generator outside the box body. An intake electromagnetic valve is arranged on the carbon dioxide inlet pipe. The control unit controls the intake electromagnetic valve according to the concentration of carbon dioxide in the box body.

5. A carbon dioxide incubator according to any one of claims 1 to 4, characterized in that: Heating pipes are arranged on the outer side of the lower half of the water jacket and below the bottom. The heating pipes are arranged in a U-shaped circuitous manner.

6. The carbon dioxide incubator according to claim 5, characterized in that: A centrifugal fan is arranged at the top inside the box body. A wind duct is arranged on the side wall on one side of the air outlet of the centrifugal fan. The air outlet is connected to the upper end of the wind duct. The lower end opening of the wind duct is located at the bottom position of the box body. The air blown out by the centrifugal fan flows from the upper end to the lower end of the wind duct and then is blown out from the lower end opening and flows through the inside of the box body to the air inlet of the centrifugal fan.

7. A carbon dioxide incubator according to claim 6, characterized in that: An efficient filter is arranged at the air inlet of the centrifugal fan.

8. A carbon dioxide incubator according to any one of claims 1 to 4, characterized in that: A plurality of shelves are arranged inside the box body, and array holes are opened on the shelves.

9. A carbon dioxide incubator according to any one of claims 1 to 4, characterized in that: The outside of the box body is covered with a heat insulation layer. A temperature sensor is arranged inside the box body. The temperature sensor is connected to the control unit. A warm water tray is also arranged at the bottom of the box body.

10. A carbon dioxide incubator according to any one of claims 1 to 4, characterized in that: An observation door is also arranged on the front side of the box body. The observation door is arranged inside the outer door. The observation door is a double-layer glass structure and an electric heating wire is arranged inside. The control unit controls the heating wire to work.