Combined heating tank and cell culture equipment
Through the design of a combined heating tank, the combination of independent electric heating film and heat insulation rings is used to solve the problem of temperature instability during small and full volume cultivation, efficient and accurate temperature control is achieved, and equipment cost and floor area are reduced.
Patent Information
- Application Number
- CN202422261208.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-09-14
AI Technical Summary
The prior art is difficult to keep the temperature of the culture medium constant during small and full volume culture processes, and the conventional temperature control method is high in cost or poor in temperature control accuracy, especially when the culture volume changes, it is difficult to quickly reach a stable state.
The combined heating tank design is adopted, including the upper tank body and the lower tank body. Each tank body is equipped with an independent electric heating film and isolate the heat transfer through an insulating ring, allowing the heating of each section of the tank body to be independently controlled to meet the needs of different volumes of culture.
It is possible to reliably maintain the stability of the culture medium temperature during small and full volume culture, improve the accuracy and efficiency of temperature control, and reduce the equipment footprint and cost.
Smart Images
Figure CN223150574U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of cell culture, in particular to a combined heating tank and a cell culture device for maintaining the cell temperature during the culture process. Background Art
[0002] A bioreactor is an appropriate environment or engineering equipment that provides a biochemical reaction. Generally, it refers to a device that uses enzymes or organisms (microorganisms) to make the device have the function of simulating organisms, and can carry out biochemical reactions outside cells. Aerobic reactions and anaerobic reactions can be carried out during the simulation process. In terms of source, it can be divided into natural bioreactors and engineering bioreactors, etc. Bioreactors are usually cylindrical, with volumes ranging from several liters to several cubic meters, and are often made of stainless steel or borosilicate glass.
[0003] To maintain the normal growth of cultured cells, a constant and appropriate temperature must be provided. Since the culture volume is generally small at the beginning of the culture in a bioreactor, the culture volume will be gradually increased by replenishing the liquid during the culture process. Sometimes, during the entire culture process, the initial culture volume and the maximum culture volume can even differ by more than 10 times. During this process, it is required that the temperature of the culture solution always remains constant or quickly reaches a stable state after experiencing a short-term fluctuation and then remains constant. One of the conventional temperature control methods is jacketed tank water circulation temperature control, that is, the culture container is a double-layer or multi-layer container. The innermost layer container is the culture solution, and the second layer is the jacket. When culturing cells, the jacket is filled with liquid water, and heat is conducted to the culture solution in the inner layer through the liquid water in the jacket to achieve temperature control of the culture solution. The disadvantage of the jacket water temperature control method is that it is difficult to simultaneously meet the requirements for constant temperature control in small-volume culture and full-volume culture, and the jacket water temperature control scheme has a high cost and a large floor area for the equipment. Another conventional temperature control method is that the culture container is a single-layer container, and a heating film is wrapped around the outer wall of the container. Heat is transferred to the culture solution by heating the heating film to achieve temperature control of the culture solution. The disadvantage of this temperature control method is that the temperature control accuracy is poor. Especially for the same tank, it is difficult to balance the temperature stability during small-volume culture and full-volume culture, and the time for the temperature to reach stability is also relatively long. Summary of the Invention
[0004] To solve the above problems, the utility model provides a combined heating tank, which includes an upper tank body and a lower tank body. A first electric heating film is arranged on the upper tank body, and a second electric heating film is arranged on the lower tank body. The first electric heating film and the second electric heating film work independently, and a heat insulation ring is arranged between the upper tank body and the lower tank body.
[0005] Further, the first electric heating film is arranged on the inner wall and / or the outer wall of the upper tank body.
[0006] Further, the second electric heating film is arranged on the inner wall and / or outer wall of the lower tank body.
[0007] Further, a plurality of through holes are respectively provided on the wall surfaces of the upper tank body and the lower tank body.
[0008] The utility model also relates to a cell culture device, comprising a device base, a device frame, a lifting and rotating device, a motor assembly, a tank bracket, and the above-mentioned combined heating tank;
[0009] The device frame is fixedly installed on one side of the device base. A tank bracket is arranged below the device frame. The combined heating tank is placed on the tank bracket. The lifting and rotating device is installed on the upper side of the device frame and can move up and down relative to the device frame; the combined heating tank is used to accommodate a cell culture bag.
[0010] Further, the cell culture device further comprises an upper pipeline bracket and a lower pipeline bracket. The upper pipeline bracket is used to support and connect the functional pipelines at the upper end of the cell culture bag, and the lower pipeline bracket is used to support and connect the functional pipelines at the lower end of the cell culture bag.
[0011] Further, a stirring paddle is arranged in the cell culture bag, and the motor assembly is used to drive the stirring paddle to rotate.
[0012] The utility model provides a combined tank design scheme, that is, the tank for supporting the cell culture container is designed in a segmented manner, and a heating film is coated on the outer surface of each section of the tank. The heating amount of each section of the tank can be independently controlled, and there is a heat insulation layer between each section of the tank, which are independent of each other. When the volume of the culture medium in the culture container is small, only the lowermost section of the tank can be heated, and the other tanks are not heated, so that the temperature control stability of the small volume can be reliably achieved; when culturing at full volume, the upper and lower sections of the tank can be heated simultaneously, so that the temperature control stability of the full volume can be quickly achieved. Description of the Drawings
[0013] Figure 1 is the overall appearance diagram of the device.
[0014] Figure 2 is the schematic diagram of the combined tank.
[0015] Figure 3 is the cross-sectional view of the combined tank. Detailed Embodiments
[0016] Figure 1 The disposable cell culture device comprises a device base 1, a device frame 2, a lifting and rotating device 3, a motor assembly 4, a tank bracket 5, a combined heating tank 6, a cell culture bag 7, an upper pipeline bracket 8, and a lower pipeline bracket 9.
[0017] The equipment base 1 is located at the bottom and serves as the chassis of the equipment. Various electrical control components and control circuits are installed inside. The equipment frame 2 is fixedly installed on one side of the equipment base 1. A tank bracket 5 is provided below the equipment frame 2. The combined heating tank 6 is located on the tank bracket 5. The lifting and rotating device 3 is installed on the upper side of the equipment frame and can move up and down relative to the equipment frame 2 through a sliding module.
[0018] The cell culture bag 7 is used to hold the cell liquid and can be placed in the combined heating tank 6. The combined tank body is located inside the combined heating tank 6 and is used to heat and keep warm the cell culture bag 7, so as to accurately control the temperature of the culture medium in the cell culture bag 7.
[0019] The lifting and rotating device 3 is used to control the height and position of the motor assembly 4 relative to the tank body. For example, when placing or taking out the cell culture bag 7, the motor assembly 4 is moved upward and rotated to one side of the tank body to facilitate the cell culture bag 7 to be put into the combined heating tank 6 or taken out from the combined heating tank 6. After the cell culture bag 7 is placed in the combined heating tank 6, the lifting and rotating device 3 controls the motor assembly 4 to be located above the cell culture bag 7, and the motor assembly 4 drives the stirring paddle (not shown) inside the cell culture bag 7.
[0020] The upper pipeline bracket 8 is used to support and connect the functional pipelines at the upper end of the cell culture bag 7, and the lower pipeline bracket 9 is used to support and connect the functional pipelines at the lower end of the cell culture bag 7.
[0021] See Figures 2 - 3 , the combined heating tank 6 includes an upper tank body 61 and a lower tank body 62. A heat insulation ring 63 is provided between the upper tank body and the lower tank body 62. A first electric heating film is arranged on the upper tank body 61, and a second electric heating film is arranged on the lower tank body 62. The first electric heating film and the second electric heating film work independently to control the temperatures of the upper tank body and the lower tank body respectively. The first electric heating film and the second electric heating film can be arranged on the inner wall and / or outer wall of the tank body.
[0022] A plurality of through holes 64 are also respectively provided on the wall surfaces of the upper tank body 61 and the lower tank body 62 for the pipeline layout of the disposable cell culture bag 7. The heat insulation ring 63 is used to isolate the upper tank body 61 and the lower tank body 62 to prevent heat transfer between them when they are in direct contact, so as to achieve precise temperature control.
[0023] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A combined heating tank, characterized in that: It includes an upper tank body (61) and a lower tank body (62). A first electric heating film is arranged on the upper tank body (61), and a second electric heating film is arranged on the lower tank body (62). The first electric heating film and the second electric heating film work independently. A heat insulation ring (63) is provided between the upper tank body and the lower tank body (62).
2. The combined heating tank according to claim 1, wherein: The first electric heating film is arranged on the inner wall and / or outer wall of the upper tank body (61).
3. The combined heating tank according to claim 1, wherein: The second electric heating film is arranged on the inner wall and / or outer wall of the lower tank body (62).
4. The combined heating tank according to claim 1, characterized in that: A plurality of through holes (64) are respectively provided on the wall surfaces of the upper tank body (61) and the lower tank body (62).
5. A cell culture device, comprising a device base (1), a device frame (2), a lifting and rotating device (3), a motor assembly (4), and a tank bracket (5), characterized in that, It also includes the combined heating tank according to any one of claims 1-4; The equipment frame (2) is fixedly installed on one side of the equipment base (1). A tank body bracket (5) is arranged below the equipment frame (2). The combined heating tank is placed on the tank body bracket (5). The lifting and rotating device (3) is installed on the upper side of the equipment frame and can move up and down relative to the equipment frame (2). The combined heating tank is used to accommodate the cell culture bag (7).
6. The cell culture device according to claim 5, characterized in that, The cell culture equipment further includes an upper pipeline bracket (8) and a lower pipeline bracket (9). The upper pipeline bracket (8) is used to support and connect the functional pipelines at the upper end of the cell culture bag (7), and the lower pipeline bracket (9) is used to support and connect the functional pipelines at the lower end of the cell culture bag (7).
7. The cell culture device according to claim 5, characterized in that, A stirring paddle is arranged in the cell culture bag (7), and the motor assembly (4) is used to drive the stirring paddle to rotate.