Micro-suction tube fine-adjustment support for cell patch forceps

Abstract

The utility model relates to the technical field of micro-fluidic equipment, in particular to a micropipette fine-tuning bracket for a pair of cell membrane forceps, which comprises a microscopic device, an intubation tube assembly is arranged on one side of the microscopic device base and comprises a frame plate fixedly connected with the objective table, the frame plate is rotationally connected with a box body, a driving shaft is movably connected into the box body, a first gear and a second gear are fixedly connected to the driving shaft, and a transmission shaft is rotationally connected into the box body; the transmission shaft is fixedly connected with a third gear and a fourth gear and provided with a micro suction pipe through a lead screw unit. According to the micro-pipette fine-tuning bracket for the cell patch forceps, the micro-pipette can be quickly close to cells and slowly and stably cling to the cells, so that the problem that the cells are pushed away due to shaking of the micro-pipette caused by subjective factors such as proficiency during manual operation is finally avoided, the time of manual operation is saved, and the working efficiency is improved. And the quality requirement of a user is lowered, the working efficiency is improved, and practicability is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic devices, in particular to a fine-tuning bracket for a micro-pipette used in cell patch clamp. Background Art

[0002] The patch clamp technique is currently the gold standard for measuring cell ion channels and membrane potential. It refers to placing a glass microelectrode with a tip diameter of about 1 micron on the cell membrane surface, achieving a tight seal between the electrode tip and the cell membrane through negative pressure suction, making the inside and outside of the electrode electrically insulated at the connection position between the electrode tip and the cell membrane. On this basis, the fixed control of the cell membrane potential is realized, and then the current of one or several ion channels on a small piece of cell membrane with an area of only a few square microns at the electrode tip is recorded, so as to achieve the purpose of recording and studying single protein ion channels.

[0003] At present, the patch clamp technique of manually operating glass microelectrodes requires professional and skilled technicians. Moreover, a well-trained professional technician can only capture about 15 cells in a day. The target cell may move during the operation due to the shaking of the human hand, resulting in the need to continuously adjust the position of the human hand. This has relatively high requirements for the comprehensive quality and proficiency of the staff, with high operation costs, time-consuming and laborious, and low work efficiency. Content of the Utility Model

[0004] The utility model provides a fine-tuning bracket for a micro-pipette used in cell patch clamp, which is used to solve the defects in the prior art that the target cell may move during the operation due to the shaking of the human hand, resulting in the need to continuously adjust the position of the human hand, with relatively high requirements for the comprehensive quality and proficiency of the staff, high operation costs, time-consuming and laborious, and low work efficiency.

[0005] The utility model provides a fine-tuning bracket for a micro-pipette used in cell patch clamp, including: a microscopic device, a stage is installed at the bottom of the microscopic device, and a base is fixedly connected to the bottom end of the stage; further including:

[0006] An intubation assembly is arranged on one side of the base. The intubation assembly includes a frame plate fixedly connected to the stage. A box body is rotatably connected to the middle of the frame plate. A rotatable drive shaft is movably connected above the box body. A first gear and a second gear are fixedly connected to the drive shaft. A transmission shaft is rotatably connected below the box body. A third gear and a fourth gear are fixedly connected to the transmission shaft. The transmission shaft installs a micro-pipette through a lead screw unit.

[0007] Wherein, in the first state, the second gear is meshed with the fourth gear; in the second state, the first gear is meshed with the third gear.

[0008] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, a limiting sleeve is penetrated and connected in the middle of the driving shaft, the limiting sleeve is fixed inside the box body, a limiting groove is opened on the limiting sleeve, a limiting post is slidably connected inside the limiting groove, and the limiting post is fixedly connected with the driving shaft;

[0009] Wherein, when the limiting post slides in the limiting groove to the first state, the second gear is meshed and connected with the fourth gear; when the limiting post slides in the limiting groove to the second state, the first gear is meshed and connected with the third gear.

[0010] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, the diameter of the first gear is smaller than that of the second gear, the diameter of the first gear is the same as that of the fourth gear, and the diameter of the second gear is the same as that of the third gear.

[0011] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, the lead - screw unit includes: a push screw and a threaded tube;

[0012] Wherein, one end of the push screw is fixedly connected to one end of the transmission shaft close to the stage, the threaded tube is sleeved outside the push screw, the threaded tube is fixedly connected with the box body, the push screw and the threaded tube are rotatably connected to each other, and a screw sleeve is sleeved on one end of the push screw.

[0013] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, a slide bar is fixedly connected to the outside of the screw sleeve, a groove rail is fixedly connected to the inside of the threaded tube, the slide bar and the groove rail are slidably connected to each other, a connecting rod is also fixedly connected to the side of the screw sleeve, the connecting rod extends out of the threaded tube and is fixedly connected with an extension rod, and the extension rod is used for installing the micro - pipette.

[0014] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, a limiting block is fixedly connected to the driving shaft, an elastic member is fixedly connected inside the box body, a resisting block is fixedly connected to the end of the elastic member, and the resisting block is lapped with the limiting block;

[0015] Wherein, when the limiting block is on one side of the resisting block, it is in the first state; when the limiting block is on the other side of the resisting block, it is in the second state.

[0016] According to the micro - pipette fine - tuning bracket for cell patch - clamp provided by the present utility model, a folding crank is installed at the outer end of the driving shaft.

[0017] According to the micro pipette fine adjustment bracket for cell patch clamp provided by the present utility model, an adjustment mechanism is arranged outside one side of the frame plate. The adjustment mechanism includes a bidirectional screw rod rotatably connected to the frame plate. Threaded sleeves are symmetrically sleeved on the bidirectional screw rod, and a rocker is rotatably connected to the side of the threaded sleeve.

[0018] The frame plate is also rotatably connected with a driving disk. The rocker is connected to the driving disk and is used to drive the driving disk to rotate. A support rod is fixedly connected to the middle of the driving disk. The support rod is rotatably connected to the frame plate. The support rod penetrates through the frame plate and is fixedly connected to the box body.

[0019] According to the micro pipette fine adjustment bracket for cell patch clamp provided by the present utility model, a scale bar fixedly connected to the frame plate is arranged below the driving disk, and an indicating arrow pointing to the scale bar is arranged on the outer edge of the driving disk.

[0020] According to the micro pipette fine adjustment bracket for cell patch clamp provided by the present utility model, a handle is inserted at the top end of the bidirectional screw rod.

[0021] A micro pipette fine adjustment bracket for cell patch clamp provided by the present utility model includes: a microscopic device. A stage is installed at the bottom of the microscopic device, and a base is fixedly connected to the bottom end of the stage. An intubation assembly is arranged on one side of the base. The intubation assembly includes a frame plate fixedly connected to the stage. A box body is rotatably connected to the middle of the frame plate. A driving shaft is movably connected above the interior of the box body. A first gear and a second gear are fixedly connected to the driving shaft. A transmission shaft is rotatably connected below the interior of the box body. A third gear and a fourth gear are fixedly connected to the transmission shaft. The transmission shaft installs a micro pipette through a lead screw unit. In the first state, the second gear is meshed and connected with the fourth gear, and the first gear and the third gear are disengaged from each other. In the second state, the first gear is meshed and connected with the third gear, and the second gear and the fourth gear are disengaged from each other. The micro pipette fine adjustment bracket for cell patch clamp provided by the present utility model enables the micro pipette to quickly approach the cell, slowly and stably lean against the cell, and finally avoids the problem that due to subjective factors such as proficiency during manual operation, the micro pipette shakes, resulting in the cell being pushed away. Furthermore, it saves the time of manual operation, reduces the quality requirements for users, improves work efficiency, and has practicability. Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions in the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0023] Figure 1Schematic diagram of the front cross-section of the micro-suction pipette fine adjustment bracket provided in one of the embodiments of the present utility model.

[0024] Figure 2 For the present utility model Figure 1 Schematic diagram of the enlarged structure at position A.

[0025] Figure 3 Schematic diagram of the front structure of the shelf board provided in one of the embodiments of the present utility model.

[0026] Figure 4 For the present utility model Figure 1 Schematic diagram of the enlarged structure at position B.

[0027] Figure 5 For the present utility model Figure 2 Schematic diagram of the enlarged structure at position C.

[0028] Reference numerals:

[0029] 1. Microscopic device; 101. Stage; 102. Base; 2. Insertion tube assembly; 201. Shelf board; 202. Box body; 203. Driving shaft; 204. First gear; 205. Second gear; 206. Transmission shaft; 207. Third gear; 208. Fourth gear; 209. Threaded tube; 210. Push screw; 211. Nut sleeve; 212. Groove rail; 213. Connecting rod; 214. Extension rod; 215. Micro-suction pipette; 216. Folding crank; 217. Elastic member; 218. Limiting block; 219. Limiting sleeve; 220. Limiting groove; 3. Adjusting mechanism; 301. Bi-directional screw; 302. Threaded sleeve; 303. Driving disc; 304. Rocker; 305. Scale bar. Detailed implementation manners

[0030] To make the objectives, technical solutions and advantages of the present utility model clearer, the technical solutions in the present utility model will be clearly and completely described below with reference to the accompanying drawings in the present utility model. Apparently, the described embodiments are some, but not all, of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art without making creative efforts based on the embodiments in the present utility model belong to the scope of protection of the present utility model.

[0031] In the description of the embodiments of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the embodiments of the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the embodiments of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0032] In the description of the embodiments of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "connected" and "connected to" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present utility model can be understood according to specific circumstances.

[0033] In the embodiments of the present utility model, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature can be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0034] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the embodiments of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0035] The following is combined with Figures 1 - 5Describe a fine adjustment bracket for a micropipette used in a cell patch clamp. The fine adjustment bracket for the cell patch clamp includes: a microscopy device 1, a stage 101 is installed at the bottom of the microscopy device 1, and a base 102 is fixedly connected to the bottom end of the stage 101. The microscopy device 1 can adopt equipment such as a microscope. A cell dish can be placed on the stage 101, and the base 102 is used to support the microscopy device 1, the stage 101, the rack plate 201, etc.

[0036] The fine adjustment bracket for the cell patch clamp further includes: an intubation assembly 2 is arranged on one side of the base 102. The intubation assembly 2 includes a rack plate 201 fixedly connected to the stage 101. A box body 202 is rotatably connected to the middle of the rack plate 201. A rotatable drive shaft 203 is movably connected above the box body 202. A first gear 204 and a second gear 205 are fixedly connected to the drive shaft 203. A transmission shaft 206 is rotatably connected below the box body 202. A third gear 207 and a fourth gear 208 are fixedly connected to the transmission shaft 206. The transmission shaft 206 installs a micropipette 215 through a lead screw unit. Specifically, the drive shaft 203 serves as a power source, and the rotation of its output shaft drives the corresponding gears to rotate, and then drives the transmission shaft 206 to rotate. Finally, the lead screw unit converts the rotational motion into a linear motion of the micropipette 215, thereby adjusting the position of the micropipette 215.

[0037] In addition, the present utility model can realize different states of meshing connection of gears by pushing and pulling the drive shaft 203, thereby realizing two kinds of adjustments to the movement speed of the microtube 215. In the first state, the second gear 205 is meshed with the fourth gear 208, and the first gear 204 is disengaged from the third gear 207; in the second state, the first gear 204 is meshed with the third gear 207, and the second gear 205 is disengaged from the fourth gear 208. Specifically, the diameters of the above four gears can be processed and designed, so as to adjust the different transmission ratios in the first state and the second state, and further adjust the speed of the microtube 215 moving. For example: the diameters of the second gear 205 and the third gear 207 are the same, the diameters of the fourth gear 208 and the first gear 204 are the same, and the diameter of the second gear 205 is larger than that of the fourth gear 208. Therefore, when the drive shaft 203 outputs the same rotational speed power, the rotational speed of the fourth gear 208 is faster in the first state, thereby controlling the micropipette 215 to move quickly, which is more suitable for the initial adjustment state of the micropipette 215. The micropipette 215 can quickly move into the lens of the microscopy device 1 and approach the cell, and then move the position of the cell dish so that the micropipette 215 is roughly aligned with the cell; in the second state, the rotational speed of the third gear 207 is slower, thereby controlling the micropipette 215 to move slowly, which is more suitable for the later fine adjustment state of the micropipette 215. The micropipette 215 moves stably and slowly towards the cell.

[0038] It can be seen that for the micro-pipette fine-tuning bracket for cell patch clamp provided by the present utility model, the micro-pipette 215 can quickly approach the cell and slowly and stably adhere to the cell. Finally, it avoids the problem that during manual operation, due to subjective factors such as proficiency, the micro-pipette 215 shakes, resulting in the cell being pushed away. Furthermore, it saves the time of manual operation, reduces the quality requirements for users, improves work efficiency, and has practicability.

[0039] The present utility model provides a micro-pipette fine-tuning bracket for cell patch clamp, which includes: a microscopy device 1. A stage 101 is installed at the bottom of the microscopy device 1, and a base 102 is fixedly connected to the bottom end of the stage 101; a cannulation assembly 2 is arranged on one side of the base 102. The cannulation assembly 2 includes a frame plate 201 fixedly connected to the stage 101. A box body 202 is rotatably connected to the middle of the frame plate 201. A drive shaft 203 is movably connected above the interior of the box body 202. A first gear 204 and a second gear 205 are fixedly connected to the drive shaft 203. A transmission shaft 206 is rotatably connected below the interior of the box body 202. A third gear 207 and a fourth gear 208 are fixedly connected to the transmission shaft 206. The micro-pipette 215 is installed on the transmission shaft 206 through a lead screw unit. In the first state, the second gear 205 is meshed and connected with the fourth gear 208, and the first gear 204 and the third gear 207 are disengaged from each other; in the second state, the first gear 204 is meshed and connected with the third gear 207, and the second gear 205 and the fourth gear 208 are disengaged from each other. It can be seen that for the micro-pipette fine-tuning bracket for cell patch clamp provided by the present utility model, the micro-pipette 215 can quickly approach the cell and slowly and stably adhere to the cell. Finally, it avoids the problem that during manual operation, due to subjective factors such as proficiency, the micro-pipette 215 shakes, resulting in the cell being pushed away. Furthermore, it saves the time of manual operation, reduces the quality requirements for users, improves work efficiency, and has practicability.

[0040] In one embodiment of the present utility model, a limiting sleeve 219 is connected through the middle of a driving shaft 203. The limiting sleeve 219 is fixed inside a box body 202. A limiting groove 220 is formed in the limiting sleeve 219. A limiting post is slidably connected inside the limiting groove 220, and the limiting post is fixedly connected to the driving shaft 203. Among them, when the limiting post slides in the limiting groove 220 to the first state, a second gear 205 is meshed and connected with a fourth gear 208; when the limiting post slides in the limiting groove 220 to the second state, a first gear 204 is meshed and connected with a third gear 207. In this embodiment, by providing the limiting sleeve 219 and limiting cooperation between the limiting groove 220 formed therein and the limiting post provided on the driving shaft 203, the moving direction of the driving shaft 203 is limited, avoiding the problem that the driving shaft 203 moves deviantly, resulting in gear meshing failure. Specifically, by fixing the limiting sleeve 219 on the inner wall of the box body 202, the extending direction of the limiting groove 220 should be parallel to the extending directions of the driving shaft 203 and the transmission shaft 206 to ensure the limitation of the movement of the driving shaft 203.

[0041] In one embodiment of the present utility model, the diameter of the first gear 204 is smaller than that of the second gear 205, the diameter of the first gear 204 is the same as that of the fourth gear 208, and the diameter of the second gear 205 is the same as that of the third gear 207. In this embodiment, through the processing and design of the diameters of the first gear 204, the second gear 205, the third gear 207, and the fourth gear 208, the purpose of different transmission ratios in different states is achieved, and the transmission ratio in the first state is smaller than that in the second state.

[0042] In one embodiment of the present utility model, a lead screw unit includes: a push screw 210 and a threaded tube 209. Among them, one end of the push screw 210 is fixedly connected to one end of the transmission shaft 206 close to the loading platform 101. The threaded tube 209 is sleeved outside the push screw 210. The threaded tube 209 is fixedly connected to the box body 202. The push screw 210 and the threaded tube 209 are rotatably connected to each other. A screw sleeve 211 is sleeved on one end of the push screw 210. In this embodiment, the lead screw unit is composed of the push screw 210 and the threaded tube 209. The threaded tube 209 is fixedly connected to the box body 202. The push screw 210 is connected to the transmission shaft 206 and drives it to rotate inside the threaded tube 209. The threaded tube 209 is provided with internal threads, and the push screw 210 is provided with external threads. By means of thread engagement, when the push screw 210 rotates, it is converted into a linear motion relative to the threaded tube 209 and drives the screw sleeve 211 to perform a linear motion together.

[0043] In one embodiment of the present utility model, a slide bar is fixedly connected to the outer side of the screw sleeve 211, a groove rail 212 is fixedly connected to the inner side of the threaded tube 209, the slide bar and the groove rail 212 are slidably connected to each other, a connecting rod 213 is also fixedly connected to the side of the screw sleeve 211, the connecting rod 213 extends out of the threaded tube 209 and is fixedly connected to an extension rod 214, and the extension rod 214 is used for installing a micropipette 215. In this embodiment, through the connection between the screw sleeve 211 and the extension rod 214, the micropipette 215 can be installed; moreover, a slide bar is arranged on the outer side of the screw sleeve 211, a groove rail 212 is arranged on the inner side of the threaded tube 209, and through the linear sliding connection between the slide bar and the groove rail 212, the linear movement direction of the screw sleeve 211 is defined, ensuring that it can drive the micropipette 215 to perform linear movement through the extension rod 214.

[0044] In one embodiment of the present utility model, a limiting block 218 is fixedly connected to the driving shaft 203, an elastic member 217 is fixedly connected to the inside of the box body 202, an abutting block is fixedly connected to the end of the elastic member 217, and the abutting block and the limiting block 218 are lapped. Among them, when the limiting block 218 is located on one side of the abutting block, it is in the first state; when the limiting block 218 is located on the other side of the abutting block, it is in the second state. Preferably, the elastic member 217 can adopt a spring, and through its elastic action, the lapping action between the abutting block and the limiting block 218 is realized, and the limiting block 218 is fixed on the driving shaft 203. The first state and the second state can be switched by applying an external force, for example: pushing or pulling the driving shaft 203 to overcome the elastic action of the spring, so that the limiting block 218 moves from one side of the abutting block to the other side, thereby realizing the switching function of gear meshing. In this embodiment, the spring and the abutting block are used to provide a lapping limit for the limiting block 218 to prevent the driving shaft 203 from slipping by itself, and the driving shaft 203 can only move when an external force is applied and the elastic action of the spring is overcome.

[0045] In one embodiment of the present utility model, a folding crank 216 is installed at the outer end of the driving shaft 203. On the one hand, the driving shaft 203 can be driven to rotate by rotating the folding crank 216, and the folding crank 216 can be rotated manually; on the other hand, the driving shaft 203 can be driven to move by pushing or pulling the folding crank 216, so as to switch to the first state or the second state.

[0046] In one embodiment of the present utility model, an adjusting mechanism 3 is provided outside one side of the rack plate 201. The adjusting mechanism 3 includes a bidirectional screw 301 rotatably connected to the rack plate 201. Threaded sleeves 302 are symmetrically sleeved on the bidirectional screw 301. A rocker 304 is rotatably connected to the side of the threaded sleeve 302. The rack plate 201 is also rotatably connected to a driving disk 303. The rocker 304 is connected to the driving disk 303 and is used to drive the driving disk 303 to rotate. A support rod is fixedly connected to the middle of the driving disk 303. The support rod is rotatably connected to the rack plate 201. The support rod penetrates through the rack plate 201 and is fixedly connected to the box body 202. In this embodiment, the rotation angle of the box body 202 is adjusted through the adjusting mechanism 3, and then the angles of the intubation assembly 2 and the microtube 215 are adjusted. Specifically, a bidirectional screw 301 is rotatably connected to the outside of the rack plate 201. Threaded sleeves 302 are sleeved on the bidirectional screw 301. The threaded sleeves 302 are connected to the driving disk 303 through rockers 304. The support rod at the center of the driving disk 303 rotates synchronously with it. The support rod is used to drive the box body 202 to rotate. By rotating the bidirectional screw 301, it is converted into the movement of the threaded sleeve 302 along the linear direction of the bidirectional screw 301. The threaded sleeve 302 drives the driving disk 303 through the rocker 304. The driving disk 303 drives the support rod and the box body 202 to rotate, so as to realize the angle adjustment of the intubation assembly 2 and the microtube 215, so that the microtube 215 can better point to the cells.

[0047] In one embodiment of the present utility model, a scale bar 305 fixedly connected to the rack plate 201 is provided below the driving disk 303. An indicating arrow pointing to the scale bar 305 is provided on the outer edge of the driving disk 303. Specifically, the scale bar 305 is marked with angle scales. The indicating arrow arranged on the outer edge of the driving disk 303 can point to the corresponding angle on the scale bar 305, so as to represent the current rotation angle of the microtube 215.

[0048] In one embodiment of the present utility model, a handle is inserted at the top of the bidirectional screw 301. The bidirectional screw 301 can be rotated manually by rotating the handle.

[0049] According to the above embodiment, a micro-suction pipe fine-tuning bracket for a cell patch clamp provided by the present utility model has the following specific use process:

[0050] First, by pulling the folding crank 216, the drive shaft 203 is pulled outwards, causing the limiting block 218 to engage with the abutting block, so that the second gear 205 meshes with the fourth gear 208. Then, the drive shaft 203 is rotated by the folding crank 216, so that the second gear 205 drives the transmission shaft 206 to rotate through the fourth gear 208. When the transmission shaft 206 rotates, the push screw 210 will rotate, causing the screw sleeve 211 to move the extension rod 214 along the groove rail 212 through the connecting rod 213 until the micro-suction pipe 215 quickly moves into the lens of the microscopic device 1 and approaches the cell. Then, the position of the cell dish is moved so that the micro-suction pipe 215 is roughly aligned with the cell.

[0051] Then, by pushing the folding crank 216, the drive shaft 203 is pushed inwards, causing the limiting post to slide to the other end in the limiting groove 220 of the limiting sleeve 219. At the same time, the limiting block 218 presses the abutting block and moves to the other side of the spring, so that the second gear 205 disengages from the fourth gear 208 and the first gear 204 meshes with the third gear 207. At this time, rotating the folding crank 216 causes the connecting rod 213 to push the extension rod 214 to move slowly, so that the micro-suction pipe 215 moves towards the cell stably and slowly.

[0052] In summary, through the above adjustments, the micro-suction pipe 215 can quickly approach the cell, slowly and stably adhere to the cell, finally avoiding the problem that when manually operating, due to subjective factors such as proficiency, the micro-suction pipe 215 shakes, resulting in the cell being pushed away. Furthermore, it saves the time of manual operation, reduces the quality requirements for users, and has practicality.

[0053] During the above process, the handle can be synchronously shaken to rotate the bidirectional screw 301, so that the threaded sleeve 302 pulls the rocker 304, causing the drive disk 303 to pull the support rod to rotate, causing the box body 202 to rotate, and finally enabling the micro-suction pipe 215 to better point to the cell.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A micro-suction pipette fine-tuning bracket for cell patch clamp, comprising: Microscopic device (1), a stage (101) is installed at the bottom of the microscopic device (1), and a base (102) is fixedly connected to the bottom end of the stage (101); characterized in that it further comprises: A cannula assembly (2) is provided on one side of the base (102). The cannula assembly (2) includes a frame plate (201) fixedly connected to the stage (101). A box body (202) is rotatably connected to the middle of the frame plate (201). A rotatable drive shaft (203) is movably connected above the interior of the box body (202). A first gear (204) and a second gear (205) are fixedly connected to the drive shaft (203). A transmission shaft (206) is rotatably connected below the interior of the box body (202). A third gear (207) and a fourth gear (208) are fixedly connected to the transmission shaft (206). The transmission shaft (206) is provided with a micropipette (215) through a lead screw unit. Wherein, in the first state, the second gear (205) is meshed and connected with the fourth gear (208); in the second state, the first gear (204) is meshed and connected with the third gear (207).

2. The micro-pipette fine-tuning bracket for cell patch clamp according to claim 1, wherein A limiting sleeve (219) is penetrated and connected to the middle of the drive shaft (203). The limiting sleeve is fixed inside the box body (202). A limiting groove (220) is formed on the limiting sleeve (219). A limiting post is slidably connected inside the limiting groove (220). The limiting post is fixedly connected to the drive shaft (203). Wherein, when the limiting post slides in the limiting groove (220) to the first state, the second gear (205) is meshed and connected with the fourth gear (208); when the limiting post slides in the limiting groove (220) to the second state, the first gear (204) is meshed and connected with the third gear (207).

3. The micro-suction pipette fine adjustment bracket for cell patch clamp according to claim 1, wherein, The diameter of the first gear (204) is smaller than the diameter of the second gear (205). The diameter of the first gear (204) is the same as the diameter of the fourth gear (208). The diameter of the second gear (205) is the same as the diameter of the third gear (207).

4. The micro-suction pipette fine-tuning bracket for cell patch clamp according to claim 1, characterized in that, The lead screw unit includes: a push screw (210) and a threaded tube (209). Wherein, one end of the push screw (210) is fixedly connected to one end of the transmission shaft (206) close to the stage (101). The threaded tube (209) is sleeved outside the push screw (210). The threaded tube (209) is fixedly connected to the box body (202). The push screw (210) and the threaded tube (209) are rotatably connected to each other. A sleeve (211) is sleeved on one end of the push screw (210).

5. The micro-pipette fine-tuning bracket for cell membrane patch clamp according to claim 4, wherein A slide bar is fixedly connected to the outside of the screw sleeve (211), a groove rail (212) is fixedly connected to the inside of the threaded pipe (209), the slide bar and the groove rail (212) are slidably connected to each other, a connecting rod (213) is also fixedly connected to the side of the screw sleeve (211), the connecting rod (213) extends out of the threaded pipe (209) and is fixedly connected to an extension rod (214), and the extension rod (214) is used for installing a micropipette (215).

6. The micro-pipette fine-tuning bracket for cell patch clamp according to claim 1, characterized in that, A limit block (218) is fixedly connected to the drive shaft (203), an elastic member (217) is fixedly connected to the inside of the box body (202), an abutting block is fixedly connected to the end of the elastic member (217), and the abutting block abuts against the limit block (218); Wherein, when the limit block (218) is located on one side of the abutting block, it is in the first state; when the limit block (218) is located on the other side of the abutting block, it is in the second state.

7. The micro-pipette fine-tuning bracket for cell membrane patch clamp according to claim 1, characterized in that A folding crank (216) is installed at the outer end of the drive shaft (203).

8. The micro-pipette fine-tuning bracket for cell membrane patch clamp according to claim 1, characterized in that, An adjusting mechanism (3) is arranged outside one side of the mounting plate (201), the adjusting mechanism (3) includes a bidirectional screw rod (301) rotatably connected to the mounting plate (201), threaded sleeves (302) are symmetrically sleeved on the bidirectional screw rod (301), and a rocker (304) is rotatably connected to the side of the threaded sleeve (302); The mounting plate (201) is also rotatably connected to a drive disk (303), the rocker (304) is connected to the drive disk (303) and is used for driving the drive disk (303) to rotate, a support rod is fixedly connected to the middle of the drive disk (303), the support rod is rotatably connected to the mounting plate (201), and the support rod penetrates through the mounting plate (201) and is fixedly connected to the box body (202).

9. The micro-pipette fine adjustment bracket for cell membrane patch clamp according to claim 8, characterized in that A scale bar (305) fixedly connected to the mounting plate (201) is arranged below the drive disk (303), and an indicating arrow pointing to the scale bar (305) is arranged on the outer edge of the drive disk (303).

10. The micro-suction pipette fine adjustment bracket for cell patch clamp according to claim 8, characterized in that, A handle is inserted into the top end of the bidirectional screw rod (301).

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