Anti-fluorescence antibody quenching box
By designing an anti-fluorescent antibody quenching box and using a dark opaque material box and lid, the problem of visible light quenching of fluorescent secondary antibodies during the quality control process is solved, and the full light-proof quality control of fluorescent secondary antibodies is achieved, which improves the reuse rate.
Patent Information
- Application Number
- CN202422306699.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-23
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-09-23
AI Technical Summary
In the prior art, fluorescent secondary antibodies are easily quenched by visible light during the quality control process, resulting in a decrease in their utilization rate and lack of measures to avoid light throughout the whole process.
An anti-fluorescent antibody quenching box was designed, using a box body and lid made of dark opaque material, combined with a liquid inlet tube and sealing plug to ensure that the fluorescent antibody quality control process avoids light throughout and prevents fluorescent groups from quenching.
The full-process light-proof quality control of fluorescent secondary antibodies is achieved, reducing the loss of fluorescent groups and improving the reuse rate of fluorescent secondary antibodies.
Smart Images
Figure CN223154836U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidic chip applications, and specifically to a box for preventing fluorescence antibody quenching. Background Art
[0002] Microfluidic chip technology is widely used in the fields of biochemistry, environmental monitoring, laboratory medicine, immunoassay, etc. An important point in the application of microfluidic chips in immunoassay is the quality control of the chips. Only the chips covered with antibodies after quality control inspection can be used. Among them, the primary antibody binds by molecular force and does not require strict light avoidance. However, the secondary antibody used for quality control is often conjugated with a self-fluorescent group. Such secondary antibodies have strong species specificity, but are extremely prone to quenching under visible light. At present, there is no measure to achieve full light avoidance, and the loss of fluorescent secondary antibodies is inevitable, and its utilization rate will be greatly reduced. Summary of the Invention
[0003] In order to solve the problem that the fluorescent group is prone to quenching during the quality control process of the above-mentioned chip, the utility model provides a box for preventing fluorescence antibody quenching, and performs chip quality control inside the box body to reduce the loss of fluorescent secondary antibodies. The technical solution adopted by the utility model is as follows:
[0004] A box for preventing fluorescence antibody quenching, comprising a box body, a lid and a liquid inlet pipe. A groove is provided at the bottom inside the box body, and the chip can be placed upright in the groove. A through hole is provided on the lid, and the liquid inlet pipe passes through the through hole and its lower end communicates with the groove, and its upper end is higher than the surface of the lid to add fluorescent antibody. A detachable sealing plug is arranged at the upper end of the liquid inlet pipe; both the box body and the lid are made of dark opaque materials.
[0005] In the above box for preventing fluorescence antibody quenching, several groups of slots are symmetrically arranged on both sides of the groove to vertically insert and place the chip.
[0006] In the above box for preventing fluorescence antibody quenching, an outlet for discharging liquid is provided at the bottom surface of the box body, and a detachable plug is hermetically connected to the outlet.
[0007] In the above box for preventing fluorescence antibody quenching, a flow channel is further provided at the bottom of the box body for communicating the lower end of the liquid inlet pipe with the groove.
[0008] In the above box for preventing fluorescence antibody quenching, the box body and the lid can be made of PP or ABS materials.
[0009] The beneficial effect of the utility model is that when the chip is placed inside the box body, the whole process of quality control can achieve full light avoidance, reducing the quenching of the self-fluorescent group of the fluorescent secondary antibody due to visible light irradiation, thereby reducing the loss of the fluorescent secondary antibody and improving the reuse rate. Brief Description of the Drawings
[0010] Figure 1 It is a schematic diagram of the external structure of an embodiment of the utility model;
[0011] Figure 2 Schematic diagram of the internal structure of the box body of the embodiment of the present utility model;
[0012] Figure 3 Schematic diagram of the cut surface of the box body of the embodiment of the present utility model.
[0013] In the figure: 1 is the box body, 2 is the lid, 3 is the liquid inlet pipe, 11 is the groove, 12 is the flow channel, 13 is the slot, 14 is the plug, 21 is the through hole, and 4 is the chip. Specific embodiments
[0014] The technical solution of the present utility model will be introduced in detail below with reference to the accompanying drawings.
[0015] This embodiment is a box for preventing fluorescence antibody quenching, including a box body 1, a lid 2 and a liquid inlet pipe 3. The lid 2 can be opened and closed. A groove 11 is provided at the bottom inside the box body 1. A through hole 21 is provided on the lid 2. The liquid inlet pipe 3 is inserted through the through hole 21, and its lower end communicates with the groove 11 and its upper end is higher than the surface of the lid 2 to add fluorescence antibody. A detachable sealing plug is arranged at the upper end of the liquid inlet pipe 3, and the liquid inlet pipe 3 is adapted to the commonly used pipette tips at present. The chip 4 can be placed in the groove 11, and the fluorescence antibody liquid flows into the groove 11, so as to realize the light-shielding quality control process of the chip.
[0016] Further, both the box body 1 and the lid 2 are made of dark opaque materials to achieve light shielding, such as brown or black, etc., and preferably made of PP or ABS materials.
[0017] Further, a plurality of groups of slots 13 are symmetrically arranged on both sides of the groove 11 for vertically inserting and placing the chip 4. The slots 13 are communicated with the groove 11. The chip 4 is vertically inserted and placed, and its lower edge is located in the groove 11 and can be in full contact with the fluorescence antibody liquid to achieve quality control.
[0018] Further, an outlet for discharging liquid is provided on the bottom surface of the box body 1, and a detachable plug 14 is hermetically connected to the outlet to facilitate liquid discharge.
[0019] Further, a flow channel 12 is also provided at the bottom of the box body 1 for communicating the lower end of the liquid inlet pipe 3 with the groove 11, which is beneficial to the more concentrated inflow of the liquid into the groove 11 and improves the use efficiency of the fluorescence secondary antibody.
Claims
1. A fluorescence antibody quenching prevention box, characterized in that: It includes a box body (1), a lid (2) and a liquid inlet pipe (3). A groove (11) is provided at the inner bottom of the box body (1), and a chip (4) can be erected and placed in the groove (11). A through hole (21) is provided on the lid (2), and the liquid inlet pipe (3) is inserted through the through hole (21), with its lower end communicating with the groove (11) and its upper end being higher than the surface of the lid (2) for adding fluorescent antibodies. A detachable sealing plug is arranged at the upper end of the liquid inlet pipe (3); both the box body (1) and the lid (2) are made of dark opaque materials.
2. The anti-fluorescent antibody quenching box according to claim 1, wherein: A number of groups of slots (13) are symmetrically provided on both sides of the groove (11) for erecting and inserting the chip (4).
3. The anti-fluorescent antibody quenching kit according to claim 1, characterized in that: An outlet for discharging liquid is provided at the bottom surface of the box body (1), and a detachable plug (14) is hermetically connected to the outlet.
4. The anti-fluorescent antibody quenching kit according to claim 1, characterized in that: A flow channel (12) is further provided at the bottom of the box body (1) for communicating the lower end of the liquid inlet pipe (3) with the groove (11).
5. The anti-fluorescent antibody quenching kit according to claim 1, characterized in that: The box body (1) and the lid (2) can be made of PP or ABS materials.