Temperature control device for post-derivatization of high performance liquid chromatography column

Abstract

The utility model discloses a temperature control device for post-derivatization of a high performance liquid chromatography column, which comprises a vortex tube, and the vortex tube comprises an air inlet short tube, and a vortex heat tube and a vortex cold tube which are connected with the air inlet short tube in a three-way manner; the temperature control device further comprises a liquid chromatography pipeline which comprises a post-column derivation pipeline spirally wound on the outer side wall of the vortex heat pipe, a cooling pipeline spirally wound on the outer side wall of the vortex cold pipe, a liquid inlet pipeline communicated with the inlet end of the post-column derivation pipeline and a liquid outlet pipeline communicated with the outlet end of the cooling pipeline. The liquid outlet pipeline is communicated with the outlet end of the cooling pipeline; the communicating pipeline is connected with the outlet end of the post-column derivation pipeline and the inlet end of the cooling pipeline; the device has the advantages that the refrigeration and temperature rise characteristics of the vortex tube are utilized, the heating function and the cooling function are controlled at the same time, and the requirements for high temperature of post-column derivatization and low temperature of cooling before detection are met, so that the post-column derivatization and detection efficiency is effectively improved, and the energy consumption is greatly reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of laboratory instruments, and particularly relates to a temperature control device for post-column derivation of a high performance liquid chromatography column. Background Art

[0002] High performance liquid chromatography is a common method for qualitative and quantitative analysis of samples, and has wide applications in the fields of pharmaceuticals, food, environment, etc. The detectors of liquid chromatography include ultraviolet detectors, evaporative light scattering detectors, mass spectrometers and other detectors. Among them, the ultraviolet detector is the most commonly used detector. For substances without ultraviolet absorption, post-column derivation is a commonly used means. For example, post-column derivation can be carried out by reacting amino acids with ninhydrin to generate a blue-violet compound, which greatly improves the application range of liquid chromatography; sugars can be derivatized to be labeled as fluorescent or ultraviolet-emitting compounds to improve the sensitivity. Since post-column derivation is a chemical reaction of substances, it is necessary to meet two requirements of post-column derivation and pre-detection cooling in high performance liquid chromatography applications, so as to achieve the purpose of accelerating the reaction rate, reducing the delay time and avoiding the reduction of column efficiency. Therefore, it is very important to find a temperature control device for derivation.

[0003] For conventional detectors, too high temperature will lead to an increase in background noise and a decrease in detection sensitivity. Cooling after the derivatization reaction is also an important process to ensure the sensitivity of the instrument. In conventional post-column derivation methods, the heating function control and the cooling function control are often controlled by two separate modules, and the system is relatively complex and consumes a large amount of energy. Summary of the Invention

[0004] The purpose of the utility model is to provide a temperature control device for post-column derivation of a high performance liquid chromatography column, which utilizes the refrigeration and heating characteristics of a vortex tube to control the heating function and the cooling function simultaneously, meets the high temperature requirements of post-column derivation and the low temperature requirements of pre-detection cooling, thereby effectively improving the post-column derivation and detection efficiency, greatly reducing the energy consumption, and at the same time the instrument is compact, simple in structure and convenient to operate.

[0005] The technical solution of the utility model is: a temperature control device for post-column derivation of a high performance liquid chromatography column, including a vortex tube, the vortex tube includes an intake short tube, and a vortex heat pipe and a vortex cold tube that are connected to the intake short tube in a tee shape; the temperature control device further includes a liquid chromatography pipeline, the liquid chromatography pipeline includes a post-column derivation pipeline spirally wound around the outer wall of the vortex heat pipe, a cooling pipeline spirally wound around the outer wall of the vortex cold tube, a liquid inlet pipeline communicated with the inlet end of the post-column derivation pipeline, a liquid outlet pipeline communicated with the outlet end of the cooling pipeline, and a communication pipeline connecting the outlet end of the post-column derivation pipeline and the inlet end of the cooling pipeline.

[0006] As a preferred technical solution, a first heat-insulating sleeve is sleeved at the end of the eddy current heat pipe and wraps the post-column derivation pipeline and the outside of the air outlet of the eddy current heat pipe, and a second heat-insulating sleeve is sleeved at the end of the eddy current cold pipe and wraps the cooling pipeline and the outside of the air outlet of the eddy current cold pipe.

[0007] As a preferred technical solution, the outer ends of both the first heat-insulating sleeve and the second heat-insulating sleeve are closed, and an annular sealing cover is provided at each inner end of the first heat-insulating sleeve and the second heat-insulating sleeve, and a plurality of air holes and two vertically corresponding pipeline through holes are provided on each annular sealing cover.

[0008] As a preferred technical solution, an outer heat-insulating box is further included, the eddy current pipe and the liquid chromatography pipeline are arranged inside the outer heat-insulating box, and a pipeline inlet and outlet is provided at the top of the outer heat-insulating box.

[0009] As a preferred technical solution, a mesh cloth layer, an inner wall glass fiber cotton layer or an outer wall phenolic foam layer is provided on the outer side walls of both the first heat-insulating sleeve and the second heat-insulating sleeve.

[0010] As a preferred technical solution, both the first heat-insulating sleeve and the second heat-insulating sleeve are made of aluminum pipes or stainless steel pipes.

[0011] As a preferred technical solution, the diameter of the intake short pipe is 5 - 15 mm, the length is 10 - 30 mm, and the thickness is 5 - 10 mm; the diameter of the eddy current heat pipe is 5 - 20 mm, the length is 30 - 100 mm, and the thickness is 5 - 10 mm; the diameter of the eddy current cold pipe is 5 - 20 mm, the length is 100 - 200 mm, and the thickness is 5 - 10 mm.

[0012] As a preferred technical solution, the diameters of both the first heat-insulating sleeve and the second heat-insulating sleeve are 30 - 100 mm, the length is 30 - 200 mm, and the thickness is 5 - 15 mm.

[0013] As a preferred technical solution, the length of the outer heat-insulating box is 200 - 700 mm, the width is 30 - 70 mm, the height is 50 - 300 mm, and the thickness is 5 - 10 mm.

[0014] As a preferred technical solution, the liquid chromatography pipeline adopts a 1 / 16 or 1 / 32 pipeline, and its length is 1 - 3 m.

[0015] The advantages of the present utility model are:

[0016] 1. The temperature control device for post-column derivation of the high-performance liquid chromatography column of the present utility model utilizes the refrigeration and heating characteristics of the vortex tube to control the heating function and the cooling function simultaneously, meeting the high-temperature requirements of post-column derivation and the low-temperature requirements of cooling before detection, thereby effectively improving the efficiency of post-column derivation and detection, greatly reducing energy consumption, and at the same time, the instrument is compact, has a simple structure and is convenient to operate. Description of the Drawings

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. The drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0018] The present utility model will be further described below in conjunction with the drawings and embodiments:

[0019] Figure 1 It is a schematic structural diagram of the temperature control device of the present utility model;

[0020] Figure 2 It is an assembly schematic diagram of the vortex tube, liquid chromatography pipeline, first heat preservation sleeve and second heat preservation sleeve of the present utility model;

[0021] Figure 3 For Figure 2 Cross-sectional view taken along the C-C direction in

[0022] Figure 4 It is a schematic diagram of the internal gas flow direction of the vortex tube, first heat preservation sleeve and second heat preservation sleeve of the present utility model;

[0023] Wherein: 1 vortex tube, 101 intake short tube, 102 vortex heat pipe, 103 vortex cold tube;

[0024] 2 liquid chromatography pipeline, 201 post-column derivation pipeline, 202 cooling pipeline, 203 inlet pipeline, 204 outlet pipeline, 205 connecting pipeline;

[0025] 3 first heat preservation sleeve; 4 second heat preservation sleeve;

[0026] 5 annular sealing cover, 501 air outlet hole, 502 pipeline perforation;

[0027] 6 outer heat preservation box, 601 pipeline inlet and outlet. Detailed Embodiment

[0028] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are for illustrating the present utility model and not for limiting the scope of the present utility model. The implementation conditions adopted in the embodiments can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not specified are usually the conditions in conventional experiments.

[0029] Embodiment: Refer to Figures 1 to 4 , a temperature control device for post-column derivation of a high-performance liquid chromatography column, including a vortex tube 1, the vortex tube 1 includes an intake short tube 101, and a vortex heat pipe 102 and a vortex cold tube 103 that are tee-connected to the intake short tube 101; the temperature control device also includes a liquid chromatography pipeline 2, the liquid chromatography pipeline 2 includes a post-column derivation pipeline 201 spirally wound around the outer wall of the vortex heat pipe 102, a cooling pipeline 202 spirally wound around the outer wall of the vortex cold tube 103, a liquid inlet pipeline 203 communicating with the inlet end of the post-column derivation pipeline 201, a liquid outlet pipeline 204 communicating with the outlet end of the cooling pipeline 202, and a communication pipeline 205 connecting the outlet end of the post-column derivation pipeline 201 and the inlet end of the cooling pipeline 202; by utilizing the refrigeration and heating characteristics of the vortex tube 1, the control of the heating function and the cooling function is carried out simultaneously, meeting the requirements of high temperature (the temperature can reach 100 - 150 °C) for post-column derivation and low temperature (the temperature can reach 0 - -40 °C) for cooling before detection, thereby effectively improving the efficiency of post-column derivation and detection and greatly reducing energy consumption.

[0030] In this embodiment, a first heat preservation sleeve 3 is sleeved at the end of the vortex heat pipe 102, which wraps the post-column derivation pipeline 201 and the outlet of the vortex heat pipe 102. A second heat preservation sleeve 4 is sleeved at the end of the vortex cold tube 103, which wraps the cooling pipeline 202 and the outlet of the vortex cold tube 103. At the same time, the outer ends of both the first heat preservation sleeve 3 and the second heat preservation sleeve 4 are closed, and an annular sealing cover 5 is provided at each inner end of the first heat preservation sleeve 3 and the second heat preservation sleeve 4. A plurality of air holes 501 and two vertically corresponding pipeline through holes 502 are provided on each annular sealing cover 5.

[0031] In this embodiment, the temperature control device further includes an outer heat preservation box 6. The vortex tube 1 and the liquid chromatography pipeline 2 are arranged inside the outer heat preservation box 6, and a pipeline inlet and outlet 601 is provided at the top of the outer heat preservation box 6.

[0032] In this embodiment, a mesh cloth layer, an inner wall glass fiber cotton layer or an outer wall phenolic foam layer is provided on the outer side walls of both the first heat preservation sleeve 3 and the second heat preservation sleeve 4.

[0033] In this embodiment, both the first heat preservation sleeve 3 and the second heat preservation sleeve 4 are made of aluminum pipes or stainless steel pipes.

[0034] In this embodiment, the intake short pipe 101 has a diameter of 5 - 15 mm, a length of 10 - 30 mm, and a thickness of 5 - 10 mm; the vortex heat pipe 102 has a diameter of 5 - 20 mm, a length of 30 - 100 mm, and a thickness of 5 - 10 mm; the vortex cold pipe 103 has a diameter of 5 - 20 mm, a length of 100 - 200 mm, and a thickness of 5 - 10 mm; the first insulation sleeve 3 and the second insulation sleeve 4 have a diameter of 30 - 100 mm, a length of 30 - 200 mm, and a thickness of 5 - 15 mm.

[0035] In this embodiment, the outer-side insulation box 6 has a length of 200 - 700 mm, a width of 30 - 70 mm, a height of 50 - 300 mm, and a thickness of 5 - 10 mm.

[0036] In this embodiment, the liquid chromatography pipeline 2 uses a 1 / 16 or 1 / 32 pipeline, and its length is 1 - 3 m.

[0037] The above embodiments are only for illustrating the technical concept and features of the present utility model. The purpose is to enable those skilled in the art to understand the content of the present utility model and implement it accordingly, and it should not be used to limit the protection scope of the present utility model. Any equivalent changes or modifications made according to the spirit of the present utility model should be covered within the protection scope of the present utility model.

Claims

1. A temperature control device for post-column derivatization of a high-performance liquid chromatography column, comprising a vortex tube, the vortex tube including an intake short tube, and a vortex heat pipe and a vortex cold tube that are tee-connected to the intake short tube; characterized in that, The temperature control device further includes a liquid chromatography pipeline, which includes a post-column derivation pipeline spirally wound around the outer wall of the vortex heat pipe, a cooling pipeline spirally wound around the outer wall of the vortex cold pipe, a liquid inlet pipeline connected to the inlet end of the post-column derivation pipeline, a liquid outlet pipeline connected to the outlet end of the cooling pipeline, and a communication pipeline connecting the outlet end of the post-column derivation pipeline and the inlet end of the cooling pipeline.

2. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 1, wherein, A first heat preservation sleeve is sleeved at the end of the vortex heat pipe and wraps around the outer sides of the post-column derivation pipeline and the air outlet of the vortex heat pipe. A second heat preservation sleeve is sleeved at the end of the vortex cold pipe and wraps around the outer sides of the cooling pipeline and the air outlet of the vortex cold pipe.

3. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 2, wherein, The outer ends of both the first heat preservation sleeve and the second heat preservation sleeve are closed. An annular sealing cover is provided at each inner end of the first heat preservation sleeve and the second heat preservation sleeve. A number of air holes and two vertically corresponding pipeline perforations are provided on each annular sealing cover.

4. The temperature control device for post-column derivatization of high performance liquid chromatography column according to claim 1, characterized in that, The temperature control device further includes an outer heat preservation box. The vortex tube and the liquid chromatography pipeline are arranged inside the outer heat preservation box, and a pipeline inlet and outlet is provided at the top of the outer heat preservation box.

5. The temperature control device for post-column derivatization of high performance liquid chromatography column according to claim 2, characterized in that, A mesh cloth layer, an inner wall glass fiber cotton layer or an outer wall phenolic foam layer is provided on the outer side walls of both the first heat preservation sleeve and the second heat preservation sleeve.

6. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 2, wherein, Both the first heat preservation sleeve and the second heat preservation sleeve are made of aluminum pipes or stainless steel pipes.

7. The temperature control device for post-column derivatization of high performance liquid chromatography column according to claim 1, characterized in that, The diameter of the intake short pipe is 5 - 15 mm, the length is 10 - 30 mm, and the thickness is 5 - 10 mm; the diameter of the vortex heat pipe is 5 - 20 mm, the length is 30 - 100 mm, and the thickness is 5 - 10 mm; the diameter of the vortex cold pipe is 5 - 20 mm, the length is 100 - 200 mm, and the thickness is 5 - 10 mm.

8. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 2, characterized in that, The diameter of both the first heat preservation sleeve and the second heat preservation sleeve is 30 - 100 mm, the length is 30 - 200 mm, and the thickness is 5 - 15 mm.

9. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 4, characterized in that, Among them, the length of the outer heat preservation box is 200 - 700 mm, the width is 30 - 70 mm, the height is 50 - 300 mm, and the thickness is 5 - 10 mm.

10. The temperature control device for post-column derivatization of a high performance liquid chromatography column according to claim 1, wherein The liquid chromatography pipeline uses a 1 / 16 or 1 / 32 pipeline, and its length is 1 - 3 m.

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