Double-path magnetic bead blood coagulation signal measuring device

Abstract

The utility model relates to a double-path magnetic bead blood coagulation signal measuring device. The double-channel magnetic bead blood clotting signal measuring device comprises a control module and a power supply module, the power supply module supplies power, the control module is connected with a multi-channel magnetic bead amplitude detection module, a multi-channel capacitance sensor U4 of the multi-channel magnetic bead amplitude detection module is connected with the control module through an I2C bus, and an interruption and closing pin of the multi-channel capacitance sensor U4 is connected with the control module; a channel of the multi-channel capacitance sensor U4 is connected with a multi-channel signal acquisition circuit, the multi-channel signal acquisition circuit is electrically connected to the two ends of a plurality of groups of detection coils, each detection coil comprises two sub-coils which are oppositely arranged and have opposite rotation directions, the two sub-coils are connected, and the axes of the two sub-coils are perpendicular to the axis of the magnetic bead driving coil; the signal acquisition circuit comprises a capacitor connected in parallel with the two ends of the detection coil, and two grounded capacitors respectively connected with the two ends of the detection coil. No signal needs to be added to the detection coil, interference is reduced, and measurement is more accurate.

Description

Technical Field

[0001] The utility model relates to the technical field of signal measurement devices for blood coagulation detection, in particular to a dual-channel magnetic bead blood coagulation signal measurement device. Background Art

[0002] Blood coagulation detection is a common reagent and blood detection method. The commonly used detection device is a dual-channel magnetic bead blood coagulation detection device, which has 4 magnetic circuits that are pairwise opposite. Among them, 2 opposite magnetic circuits are used to generate an alternating magnetic field. The generated magnetic field can attract the magnetic beads to move towards it, and then power it off to stop generating the magnetic field. At the same time, power on another magnetic circuit opposite to it to generate a magnetic field, so that the small magnetic beads move in the opposite direction. When the 2 magnetic circuits generate an alternating magnetic field, the small magnetic beads in the reagent container are driven to move in a cycle. The other pair of magnetic circuits is used to monitor the change in the swing amplitude of the magnetic beads and generate corresponding electrical signals. When the sample undergoes a coagulation reaction due to the addition of the reagent, the viscosity of the sample mixture increases, causing the reciprocating movement amplitude of the magnetic beads to decrease. By detecting the movement amplitude of the magnetic beads, the coagulation degree of the mixture can be indirectly obtained. The two magnetic circuits for monitoring the swing amplitude of the magnetic beads are arranged on both sides of the reagent container and perpendicular to the swing direction of the magnetic beads. Apply a pulsed voltage of 10 kHz to the coil of one of the magnetic circuits. As a result, an alternating magnetic field is generated in the coil, and an alternating induced voltage of 10 kHz is generated in the coil at the other end due to the induction of the magnetic field change. As the small magnetic beads reciprocate and continuously cut the magnetic force lines, an amplitude modulation below 10 Hz is generated for the induced 10 kHz carrier wave. The change in the carrier wave amplitude is proportional to the movement amplitude of the small magnetic beads. The subsequent stage of the detection circuit amplifies, demodulates, and low-pass filters the received signal to obtain the modulated low-frequency signal, and calculates the coagulation degree of the plasma sample through the change in the signal amplitude. The current disadvantages of the existing technology are that the detection device uses a relatively complex analog circuit design. Because the amplitude of the induced signal is low, multiple operational amplifiers are required to amplify and process the signal, so additional noise interference will be introduced and the signal-to-noise ratio is low; because the detection end needs to obtain the detected electrical signal through electromagnetic induction, it is easily affected by the electromagnetic radiation in the surrounding environment and has weak anti-interference ability; in addition, because the circuit requires more components, the consistency is poor and the debugging is difficult due to the influence of the processing technology in actual production, resulting in a large deviation in the results. Content of the Utility Model

[0003] In order to solve the above technical problems or at least partially solve the above technical problems, the utility model provides a dual-channel magnetic bead blood coagulation signal measurement device.

[0004] The utility model provides a dual-channel magnetic bead blood coagulation signal measuring device, comprising: a control module and a power supply module. The power supply module supplies power, and the control module is connected to a multi-channel magnetic bead amplitude detection module. The multi-channel magnetic bead amplitude detection module includes: a multi-channel capacitance sensor U4, which is connected to the control module through an I2C bus. The interrupt and shutdown pins of the multi-channel capacitance sensor U4 are connected to the control module; the channels of the multi-channel capacitance sensor U4 are connected to a multi-channel signal acquisition circuit, and multiple signal acquisition circuits are electrically connected to both ends of multiple groups of detection coils. Among them, the detection coil includes two sub-coils with opposite rotation directions arranged oppositely, the two sub-coils are connected, and the axes of the two sub-coils are perpendicular to the axis of the magnetic bead driving coil; the signal acquisition circuit includes: a capacitor connected in parallel to both ends of the detection coil, and two capacitors grounded respectively connected to both ends of the detection coil.

[0005] Furthermore, the power supply module includes: an AC-DC conversion circuit that converts 220V AC into 24V DC. The output of the AC-DC conversion circuit is connected to a fuse. The output of the AC-DC conversion circuit is connected to a voltage stabilizing diode and a filtering circuit. The AC-DC conversion circuit inputs 24V voltage to a first DC-DC power supply chip U1 and a second DC-DC power supply chip U2. The first DC-DC power supply chip U1 converts 24V voltage into 12V voltage, and the second DC-DC power supply chip U2 converts 24V voltage into 5V voltage; the output of the second DC-DC power supply chip U2 is electrically connected to a third DC-DC power supply chip U3, and the third DC-DC power supply chip U3 converts 5V voltage into 3.3V voltage.

[0006] Furthermore, the output terminals of the first DC-DC power supply chip U1, the second DC-DC power supply chip U2, and the third DC-DC power supply chip U3 are connected to filtering capacitors and status indicator LEDs.

[0007] Furthermore, the control module is connected to a CAN communication module. The CAN communication module includes a CAN transceiver chip U5. The CAN transceiver chip U5 is powered by 5V voltage. The CAN bus pins of the CAN transceiver chip U5 are connected to ESD diodes for surge protection. Two CAN buses are led out from the CAN bus pins of the CAN transceiver chip U5. The CAN communication module is connected to a host computer.

[0008] Further, the control module is electrically connected to a plurality of bead driving circuits. Each bead driving circuit includes: two optocouplers, the light-emitting ends of each optocoupler are respectively connected to the control module, the light-receiving ends of the two optocouplers are respectively connected to two voltage-dividing resistor circuits, each voltage-dividing resistor circuit is arranged between a 12V power supply and the ground and includes two resistors; the two resistors between the two voltage-dividing resistor circuits are respectively connected to the gates of two field-effect transistors, the source of each field-effect transistor is grounded, the drain of each field-effect transistor is respectively connected to one end of a bead driving coil, and the other end of each bead driving coil is connected to the 12V power supply.

[0009] Further, an LED indicator circuit is arranged at the drain of each field-effect transistor. The LED indicator circuit includes a resistor and an LED indicator connected in series, and a reverse diode connected in parallel with the resistor and the LED indicator. The LED indicator circuit is connected to the 12V power supply.

[0010] Further, two bead driving coils and a detection coil form a set of measurement units, and the two sub-coils of the detection coil and the two bead driving coils are arranged in a cross shape.

[0011] The above technical solutions provided by the embodiments of the present invention have the following advantages compared with the prior art:

[0012] The channels of the multi-channel capacitance sensor U4 are connected to a multi-channel signal acquisition circuit, and the multi-channel signal acquisition circuit is electrically connected to both ends of multiple groups of detection coils. Among them, the detection coil includes two sub-coils with opposite rotation directions arranged oppositely, the two sub-coils are connected, and the axes of the two sub-coils are perpendicular to the axis of the bead driving coil; the signal acquisition circuit includes: a capacitor connected in parallel to both ends of the detection coil, and two capacitors grounded respectively connected to both ends of the detection coil. The change in the swing amplitude of the bead changes the inductance value of the loop where the detection coil is located, and the change in the inductance value causes the frequency of the LC oscillation in the loop where the detection coil is located to change. The greater the swing amplitude of the bead, the greater the deviation of the oscillation frequency. The multi-channel capacitance sensor U4 obtains this oscillation frequency and outputs it to the control module through the I2C bus, and the control module calculates and infers the swing amplitude of the bead according to the change amplitude of the oscillation frequency. This application does not require a 10KHZ signal for the detection coil, greatly reducing the interference of other external signals, and uses a digital processing chip and a small amount of peripheral circuits to detect the change in inductance, reducing interference. After the control module obtains the data through the I2C bus, it uses FFT signal filtering to obtain the movement amplitude of the bead, and the result obtained is more accurate. Description of the Drawings

[0013] The drawings here are incorporated into the specification and form a part of this specification, showing the embodiments consistent with the present invention, and are used together with the specification to explain the principles of the present invention.

[0014] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, for those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0015] Figure 1 It is a schematic diagram of a dual-channel magnetic bead blood coagulation signal measurement device provided by an embodiment of the present invention.

[0016] Figure 2 It is a schematic diagram of a power supply module provided by an embodiment of the present invention;

[0017] Figure 3 It is a schematic diagram of a control module provided by an embodiment of the present invention;

[0018] Figure 4 It is a schematic diagram of a CAN communication module provided by an embodiment of the present invention;

[0019] Figure 5 It is a schematic diagram of a multi-channel magnetic bead amplitude detection module provided by an embodiment of the present invention;

[0020] Figure 6 It is a schematic diagram of a magnetic bead drive circuit provided by an embodiment of the present invention;

[0021] Figure 7 It is a schematic diagram of the circuit connection between a detection coil and a magnetic bead drive coil provided by an embodiment of the present invention;

[0022] Figure 8 It is a schematic diagram of a detection coil and a magnetic bead drive coil provided by an embodiment of the present invention. Detailed implementation manners

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are some but not all of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0024] It should be noted that in this text, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not explicitly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising one..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the said element.

[0025] Referring to Figure 1 as shown, an embodiment of the present utility model provides a dual-channel magnetic bead blood coagulation signal measurement device, comprising:

[0026] a power supply module, as Figure 2 shown, the power supply module includes: an AC-to-DC circuit that converts 220V AC into 24V DC, the output of the AC-to-DC circuit is connected to a fuse, the output of the AC-to-DC circuit is connected to a zener diode and a filter circuit, the AC-to-DC circuit inputs 24V voltage to a first DC-to-DC power supply chip U1 and a second DC-to-DC power supply chip U2. The first DC-to-DC power supply chip U1 converts 24V voltage into 12V voltage, and the second DC-to-DC power supply chip U2 converts 24V voltage into 5V voltage; an exemplary model of the first DC-to-DC power supply chip U1 is K7812-1000R3, and an exemplary model of the second DC-to-DC power supply chip U2 is K7805-1000R3. The output of the second DC-to-DC power supply chip U2 is electrically connected to a third DC-to-DC power supply chip U3, and the third DC-to-DC power supply chip U3 converts 5V voltage into 3.3V voltage. An exemplary model of the third DC-to-DC power supply chip U3 is AMS117-3.3. The output terminals of the first DC-to-DC power supply chip U1, the second DC-to-DC power supply chip U2 and the third DC-to-DC power supply chip U3 are connected to filter capacitors and status indicator LEDs. The power supply module supplies power to the dual-channel magnetic bead blood coagulation signal measurement device.

[0027] a control module, as Figure 3 shown, the control module uses an STM32 single-chip microcomputer, and the control module is configured with a reset circuit and a crystal oscillator circuit.

[0028] The control module is connected to a CAN communication module, as Figure 4As shown in the figure, an exemplary CAN communication module uses a CAN transceiver chip U5 of model TJA1050T. The CAN transceiver chip U5 is powered by a second DC-DC power supply chip U2. The CAN bus pins of the CAN transceiver chip U5 are connected to ESD diodes for surge protection. Two CAN buses are led out from the CAN bus pins of the CAN transceiver chip U5. The CAN communication module is connected to a host computer. The host computer is configured with a display, and the bead amplitude condition is displayed through the display.

[0029] As Figure 5 shown in the figure, the control module is connected to a multi-channel bead amplitude detection module. The multi-channel bead amplitude detection module includes: a multi-channel capacitance sensor U4. The multi-channel capacitance sensor U4 is connected to the control module through an I2C bus. The interrupt and shutdown pins of the multi-channel capacitance sensor U4 are connected to the control module. The channels of the multi-channel capacitance sensor U4 are connected to a multi-channel signal acquisition circuit. The multi-channel signal acquisition circuits are electrically connected to both ends of multiple groups of detection coils. The signal acquisition circuit includes: a capacitor connected in parallel to both ends of the detection coil, and two capacitors grounded and respectively connected to both ends of the detection coil.

[0030] As Figure 6 shown in the figure, the control module is electrically connected to a multi-channel bead driving circuit. Each bead driving circuit includes: two optocouplers. The light-emitting ends of each optocoupler are respectively connected to the control module. The light-receiving ends of the two optocouplers are respectively connected to two voltage-dividing resistor circuits; each voltage-dividing resistor circuit is arranged between a 12V power supply and the ground and includes two resistors; the two resistors between the two voltage-dividing resistor circuits are respectively connected to the gates of two field-effect transistors; the source of each field-effect transistor is grounded, and the drain of each field-effect transistor is respectively connected to one end of a bead driving coil. Figure 7 As shown in the figure, the other end of each bead driving coil is connected to a 12V power supply. An LED indicator circuit is arranged at the drain of each field-effect transistor. The LED indicator circuit includes a resistor and an LED indicator connected in series, and a reverse diode connected in parallel with the resistor and the LED indicator. The LED indicator circuit is connected to a 12V power supply.

[0031] As Figure 8 shown in the figure, two bead driving coils and one detection coil form a set of measurement units, and the two sub-coils of the detection coil and the two bead driving coils are arranged in a cross shape.

[0032] The control module receives the capacitance data of the multi-channel capacitance sensor U4, then filters and shapes the collected data using the FFT algorithm, and then outputs it to the host computer through the CAN communication module.

[0033] The working principle of this application is as follows:

[0034] The change in the swing amplitude of the magnetic bead changes the inductance value of the loop where the detection coil is located. The change in the inductance value causes the frequency of the LC oscillation in the loop where the detection coil is located to change. The larger the swing amplitude of the magnetic bead, the greater the deviation of the oscillation frequency. The multi-channel capacitance sensor U4 obtains this oscillation frequency and outputs it to the control module through the I2C bus. The control module calculates and infers the swing amplitude of the magnetic bead based on the change amplitude of the oscillation frequency. Usually, the swing dropping to half of the initial value is taken as the end point of sample solidification. This application does not require a 10KHZ signal for the detection coil, greatly reducing the interference of other external signals. It uses a digital processing chip and a small amount of peripheral circuits to detect the change in inductance and reduce interference. After the control module obtains data through the I2C bus, it uses FFT signal filtering to obtain the movement amplitude of the magnetic bead, and the result obtained is more accurate.

[0035] In the embodiments provided by the present utility model, it should be understood that the disclosed structure can be implemented in other ways. For example, the structural embodiments described above are only illustrative. For example, the division of the units is only a logical function division. In actual implementation, there may be other division methods. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed coupling or direct coupling or communication connection to each other can be through some interfaces. The indirect coupling or communication connection of the structure or unit can be in electrical, mechanical or other forms.

[0036] The units described as separate components may or may not be physically separated. The components displayed as units may or may not be physical units, that is, they can be located in one place, or they can be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0037] In addition, the functional units in each embodiment of the present utility model can be integrated in a processing unit, or each unit can exist physically alone, or two or more units can be integrated in one unit. The above integrated units can be implemented in the form of hardware or in the form of software functional units.

[0038] The above are only the specific implementation manners of the present utility model, enabling those skilled in the art to understand or implement the present utility model. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present utility model. Therefore, the present utility model will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A dual-channel magnetic bead blood coagulation signal measurement device, characterized in that, Comprising: A control module and a power supply module. The power supply module supplies power. The control module is connected to a multi-channel bead amplitude detection module. The multi-channel bead amplitude detection module includes: a multi-channel capacitance sensor U4. The multi-channel capacitance sensor U4 is connected to the control module through an I2C bus. The interrupt and shutdown pins of the multi-channel capacitance sensor U4 are connected to the control module. The channels of the multi-channel capacitance sensor U4 are connected to a multi-channel signal acquisition circuit. The multi-channel signal acquisition circuits are electrically connected to both ends of multiple groups of detection coils. Among them, the detection coil includes two sub-coils with opposite rotation directions arranged oppositely. The two sub-coils are connected. The axes of the two sub-coils are perpendicular to the axis of the bead drive coil. The signal acquisition circuit includes: a capacitor connected in parallel to both ends of the detection coil, and two capacitors grounded and respectively connected to both ends of the detection coil.

2. The dual-channel magnetic bead blood coagulation signal measurement device according to claim 1, wherein The power supply module includes: an AC-DC conversion circuit that converts 220V AC into 24V DC. The output of the AC-DC conversion circuit is connected to a fuse. The output of the AC-DC conversion circuit is connected to a zener diode and a filter circuit. The AC-DC conversion circuit inputs 24V voltage to a first DC-DC power supply chip U1 and a second DC-DC power supply chip U2. The first DC-DC power supply chip U1 converts 24V voltage into 12V voltage. The second DC-DC power supply chip U2 converts 24V voltage into 5V voltage. The output of the second DC-DC power supply chip U2 is electrically connected to a third DC-DC power supply chip U3. The third DC-DC power supply chip U3 converts 5V voltage into 3.3V voltage.

3. The dual-channel magnetic bead blood coagulation signal measuring device according to claim 2, wherein The output terminals of the first DC-DC power supply chip U1, the second DC-DC power supply chip U2, and the third DC-DC power supply chip U3 are connected to filter capacitors and status indicator LEDs.

4. The dual-channel magnetic bead blood coagulation signal measurement device according to claim 1, characterized in that, The control module is connected to a CAN communication module. The CAN communication module includes a CAN transceiver chip U5. The CAN transceiver chip U5 is powered by 5V voltage. The CAN bus pins of the CAN transceiver chip U5 are connected to ESD diodes for surge protection. Two CAN buses are led out from the CAN bus pins of the CAN transceiver chip U5. The CAN communication module is connected to a host computer.

5. The dual-channel magnetic bead blood coagulation signal measuring device according to claim 1, wherein The control module is electrically connected to a multi-channel bead drive circuit. Each bead drive circuit includes: two optocouplers. The light-emitting ends of each optocoupler are respectively connected to the control module. The light-receiving ends of the two optocouplers are respectively connected to two voltage-dividing resistor circuits. Each voltage-dividing resistor circuit is arranged between a 12V power supply and ground and includes two resistors. Between the two resistors are respectively connected to the gates of two field-effect transistors. The source of each field-effect transistor is grounded. The drain of each field-effect transistor is respectively connected to one end of a bead drive coil. The other end of each bead drive coil is connected to a 12V power supply.

6. The dual-channel magnetic bead blood coagulation signal measuring device according to claim 5, wherein, An LED indicator circuit is provided at the drain of each field-effect transistor. The LED indicator circuit includes a resistor and an LED indicator connected in series, and a reverse diode connected in parallel with the resistor and the LED indicator. The LED indicator circuit is connected to a 12V power supply.

7. The dual-channel magnetic bead blood coagulation signal measurement device according to claim 5, characterized in that, Two magnetic bead driving coils and one detection coil form a set of measurement units, and the two sub-coils of the detection coil and the two magnetic bead driving coils are arranged in a cross shape.

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