Immunofluorescence double-light-source calibration control system

Abstract

The utility model relates to the technical field of fluorescence detection, in particular to an immunofluorescence double-light-source calibration control system, which comprises a control detection circuit, a first detection circuit, a second detection circuit, an adjustment calibration circuit and a signal acquisition circuit, and the first detection circuit and the second detection circuit are both electrically connected with the signal acquisition circuit. The first detection circuit or the second detection circuit is electrically connected with the adjustment calibration circuit, and the control detection circuit is electrically connected with the adjustment calibration circuit and the signal acquisition circuit. The whole automatic calibration process of the immunofluorescence double-light-source calibration control system depends on cooperation of the control detection circuit, the first detection circuit, the second detection circuit, the adjustment calibration circuit and the signal acquisition circuit, and automatic calibration of the immunofluorescence double light sources for detection can be realized. Therefore, the accuracy and the reliability of an immunofluorescence detection result are greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence detection, in particular to an immunofluorescence dual-light source calibration control system. Background Technique

[0002] The immunofluorescence detection method is a biological analysis method that combines immunology and fluorescence technology to achieve the visual detection of the target by labeling antibodies or antigens with fluorescent dyes. The immunofluorescence dual-light source detection system is a common system for quantitatively detecting specific antigens or antibodies by using the immunofluorescence method. It usually includes equipment such as a fluorescence microscope or a fluorescence analyzer, and uses two light sources with different wavelengths (usually 365nm and 410nm) to excite and detect the signal intensity and position of different fluorescent dyes, so as to observe and analyze the immunofluorescence detection results. Through the immunofluorescence dual-light source detection system, scientific researchers and clinicians can quickly and accurately detect and quantitatively analyze the presence of specific antigens or antibodies, providing an important basis for disease diagnosis and treatment. However, with the increasingly widespread application of the immunofluorescence dual-light source detection system, testers have found that the system sometimes has problems with unstable signals after a period of use. The main reason may be that the intensity and wavelength of the light source change over time, or the instrument parameters drift due to environmental influences, resulting in unstable parameters of the instrument and the light source, greatly reducing the accuracy and reliability of the immunofluorescence detection results. Content of the Utility Model

[0003] In view of this, the utility model proposes an immunofluorescence dual-light source calibration control system, aiming to solve the problem that the accuracy of the detection results of the current immunofluorescence dual-light source detection system will decrease after a period of use.

[0004] The utility model proposes an immunofluorescence dual-light source calibration control system, which includes a control detection circuit, a first detection circuit, a second detection circuit, an adjustment and calibration circuit, and a signal acquisition circuit. The first detection circuit and the second detection circuit are both electrically connected to the signal acquisition circuit. The first detection circuit or the second detection circuit is electrically connected to the adjustment and calibration circuit. The control detection circuit is respectively electrically connected to the adjustment and calibration circuit and the signal acquisition circuit.

[0005] Further, the control detection circuit includes an MCU control chip U5 of the GD32F130F8P6 type, a resistor R29, a resistor R26, a capacitor C19, a capacitor C11, a resistor R11, a resistor R32, a three-pin connector J2, a resistor R27, a resistor R28, a resistor R22, a resistor R34, an NPN-type phototransistor Q2, an NPN-type phototransistor Q3, a light-emitting diode D2, a light-emitting diode D4, and a resistor R25;

[0006] Among them, the first pin of the MCU control chip U5 is grounded through the resistor R29, the second pin, the third pin, the sixth pin and the ninth pin of the MCU control chip U5 are all floating, the fourth pin of the MCU control chip U5 is respectively connected to one end of the resistor R26 and one end of the capacitor C19, the fifth pin of the MCU control chip U5 is respectively connected to the 3.3V power supply terminal, the other end of the resistor R26 and one end of the capacitor C11, the seventh pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R11, the eighth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R32, the tenth pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q3 through the resistor R34, the eleventh pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q2 through the resistor R22, the twelfth pin of the MCU control chip U5 is connected to the MCP A0 terminal, the thirteenth pin of the MCU control chip U5 is connected to the MCP SCL terminal, the fourteenth pin of the MCU control chip U5 is connected to the MCP SDA terminal, the fifteenth pin of the MCU control chip U5 is grounded, the sixteenth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal, the seventeenth pin of the MCU control chip U5 is connected to the TX signal sending terminal through the resistor R28, the eighteenth pin of the MCU control chip U5 is connected to the RX signal receiving terminal through the resistor R27, the nineteenth pin of the MCU control chip U5 is connected to the first end of the three-pin connector J2, and the twentieth pin of the MCU control chip U5 is connected to the second end of the three-pin connector J2;

[0007] The other ends of the capacitor C11 and the capacitor C19 are both grounded, the third end of the three-pin connector J2 is grounded, the emitters of the NPN phototransistor Q2 and the NPN phototransistor Q3 are both grounded, the collector of the NPN phototransistor Q2 is connected to the negative electrode of the light-emitting diode D2, the collector of the NPN phototransistor Q3 is connected to the negative electrode of the light-emitting diode D4, the positive electrodes of the light-emitting diode D2 and the light-emitting diode D4 are both connected to one end of the resistor R25, and the other end of the resistor R25 is connected to the VCC power supply terminal.

[0008] Further, the light-emitting diode D2 is a 365nm type light-emitting diode, and the light-emitting diode D4 is a 410nm type light-emitting diode.

[0009] Further, the first detection circuit includes an operational amplifier chip U1 of type AD822AR, a light-emitting diode D1, a capacitor C1, a resistor R3, a resistor R6, a resistor R12, a capacitor C2, a resistor R4, a resistor R1, a resistor R2, a resistor R10, a resistor R31, a variable potentiometer VR1, a resistor R5, a capacitor C3, and a resistor R9;

[0010] Among them, the first pin of the operational amplifier chip U1 is connected to one end of the resistor R4, the second pin of the operational amplifier chip U1 is connected to one end of the resistor R1, the third pin of the operational amplifier chip U1 is connected to one end of the resistor R2, the fourth pin of the operational amplifier chip U1 is respectively connected to one end of the resistor R9 and the ground terminal, the fifth pin of the operational amplifier chip U1 is connected to one end of the resistor R5, the sixth pin of the operational amplifier chip U1 is connected to the other end of the resistor R9, the seventh pin of the operational amplifier chip U1 is connected to a 0.8 - 1.2V voltage signal terminal, and the eighth pin of the operational amplifier chip U1 is respectively connected to one end of the capacitor C3 and the 3.3V power supply terminal;

[0011] The other end of the resistor R4 is respectively connected to one end of the capacitor C1, one end of the resistor R3, and the other end of the resistor R5. The other end of the resistor R1 is respectively connected to the other end of the resistor R3, the other end of the capacitor C1, and the negative electrode of the light-emitting diode D1. The other end of the resistor R2 is respectively connected to one end of the resistor R10, one end of the capacitor C2, and one end of the resistor R6. The other end of the resistor R6 is respectively connected to the positive electrode of the light-emitting diode D1 and one end of the resistor R12. The other end of the resistor R12 and the other end of the capacitor C2 are both grounded. The other end of the resistor R10 is connected to one end of the variable potentiometer VR1 and the adjustment terminal of the variable potentiometer VR1 through the resistor R31. The other end of the variable potentiometer VR1 is grounded, and the other end of the capacitor C3 is grounded.

[0012] Further, the second detection circuit includes an operational amplifier chip U2 of type AD822AR, a light-emitting diode D3, a resistor R24, a resistor R30, a resistor R17, a capacitor C4, a resistor R18, a resistor R19, a resistor R20, a resistor R16, a capacitor C5, a resistor R23, and a resistor R33;

[0013] Among them, the first pin of the operational amplifier chip U2 is connected to one end of the resistor R18, the second pin of the operational amplifier chip U2 is connected to one end of the resistor R19, the third pin of the operational amplifier chip U2 is connected to one end of the resistor R20, the fourth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R23 and the ground terminal, the fifth pin of the operational amplifier chip U2 is connected to one end of the resistor R16, the sixth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R33 and the other end of the resistor R23, the seventh pin of the operational amplifier chip U2 is respectively connected to the other end of the resistor R33 and the 0.8 - 1.2V voltage signal terminal, and the eighth pin of the operational amplifier chip U2 is respectively connected to one end of the capacitor C5 and the 3.3V power supply terminal;

[0014] The other end of the resistor R18 is respectively connected to one end of the capacitor C4, the other end of the resistor R16, and one end of the resistor R17. The other end of the resistor R19 is respectively connected to the other end of the resistor R17, the other end of the capacitor C4, and the cathode of the light-emitting diode D3. The other end of the resistor R20 is respectively connected to the anode of the light-emitting diode D3 and one end of the resistor R24. The other end of the resistor R24 is grounded through the resistor R30, and the other end of the capacitor C5 is grounded.

[0015] Further, the adjustment and calibration circuit is electrically connected to the first detection circuit.

[0016] Further, the adjustment and calibration circuit includes a digital potentiometer chip U6 of the MCP4561 - 503E / MS type and a resistor R8;

[0017] Among them, the first pin of the digital potentiometer chip U6 is connected to the MCP A0 terminal, the second pin of the digital potentiometer chip U6 is connected to the MCP SCL terminal, the third pin of the digital potentiometer chip U6 is connected to the MCP SDA terminal, the fourth pin of the digital potentiometer chip U6 is grounded, the fifth pin of the digital potentiometer chip U6 is connected to one end of the resistor R8, the sixth pin of the digital potentiometer chip U6 is respectively connected to the other end of the resistor R8 and the 0.8 - 1.2V voltage signal terminal, the seventh pin of the digital potentiometer chip U6 is left floating, and the eighth pin of the digital potentiometer chip U6 is connected to the 3.3V power supply terminal.

[0018] Further, the signal acquisition circuit includes a 24 - bit analog - to - digital converter chip U4 of the HY3116 type, a resistor R7, a capacitor C17, a capacitor C14, a resistor R21, a resistor R13, a capacitor C16, a capacitor C6, and a capacitor C15;

[0019] Among them, the first pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C14 and the 3.3V power supply terminal, the second pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C14 and the ground terminal, the third pin of the 24-bit analog-to-digital converter chip U4 is connected to one end of the resistor R21, the fourth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the resistor R13 and one end of the capacitor C16, the fifth and sixth pins of the 24-bit analog-to-digital converter chip U4 are both connected to one end of the capacitor C15, the seventh pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C15 and the ground terminal, the eighth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SDA terminal, the ninth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SCL terminal, and the tenth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C6 and the 3.3V power supply terminal;

[0020] The other end of the resistor R13 is connected to the 0.8 - 1.2V voltage signal terminal, the other ends of the capacitor C16 and the capacitor C6 are both grounded, the other end of the resistor R21 is respectively connected to one end of the capacitor C17 and one end of the resistor R7, the other end of the capacitor C17 is grounded, and one end of the resistor R7 is connected to the 0.8 - 1.2V voltage signal terminal.

[0021] Further, the immunofluorescence dual-light source calibration control system further includes a power supply circuit, and the power supply circuit is electrically connected to the control and detection circuit.

[0022] Further, the power supply circuit includes a low-dropout voltage regulator chip U3 of the TLV70233DBVT type, a capacitor C9, a resistor R15, and a capacitor C10;

[0023] Among them, the first pin of the low-dropout voltage regulator chip U3 is respectively connected to one end of the capacitor C9 and the power supply terminal, the second pin of the low-dropout voltage regulator chip U3 is respectively connected to the other end of the capacitor C9 and the ground terminal, the third pin of the low-dropout voltage regulator chip U3 is connected to the power supply terminal, the fourth pin of the low-dropout voltage regulator chip U3 is left floating, the fifth pin of the low-dropout voltage regulator chip U3 is respectively connected to one end of the capacitor C10, one end of the resistor R15, and the 3.3V power supply terminal, the other end of the capacitor C10 is grounded, and the other end of the resistor R15 is connected to the 3.3V power supply terminal.

[0024] Compared with the prior art, the beneficial effects of the present utility model are as follows: An immunofluorescence dual-light source calibration control system includes a control detection circuit, a first detection circuit, a second detection circuit, an adjustment and calibration circuit, and a signal acquisition circuit. The first detection circuit and the second detection circuit are both electrically connected to the signal acquisition circuit. The first detection circuit or the second detection circuit is electrically connected to the adjustment and calibration circuit. The control detection circuit is respectively electrically connected to the adjustment and calibration circuit and the signal acquisition circuit. In this immunofluorescence dual-light source calibration control system, the signal acquisition circuit is responsible for collecting the detection signals of the first detection circuit and the second detection circuit and sending them to the control detection circuit. When the control detection circuit confirms that the numerical values of the detection signals of the first detection circuit and the second detection circuit are not equal, the adjustment and calibration circuit is triggered to change the numerical value of the detection signal of the first detection circuit or the second detection circuit until the numerical values of the detection signals of the first detection circuit and the second detection circuit are exactly equal. Once it is reconfirmed that the numerical values of the detection signals of the two detection circuits are exactly equal, it indicates that the automatic calibration process of this immunofluorescence dual-light source calibration control system is completed and it can enter the formal immunofluorescence detection stage. After applying this immunofluorescence dual-light source calibration control system to complete automatic calibration, it can effectively eliminate the influence of changes in light source intensity and wavelength and the drift of instrument parameters on the accuracy of the detection results, thereby improving the accuracy and reliability of immunofluorescence detection results and providing more reliable data support for medical diagnosis and biomedical research. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] By reading the detailed description of the preferred embodiments below, various other advantages and benefits will become clear to those of ordinary skill in the art. The drawings are only for the purpose of showing the preferred embodiments and are not considered to be a limitation of the present utility model. Moreover, throughout the drawings, the same reference numerals are used to represent the same components. In the drawings:

[0026] Figure 1 is the structural block diagram of the immunofluorescence dual-light source calibration control system provided by the embodiment of the present utility model;

[0027] Figure 2 is the circuit structure diagram of the control detection circuit provided by the embodiment of the present utility model;

[0028] Figure 3 is the circuit structure diagram of the first detection circuit provided by the embodiment of the present utility model;

[0029] Figure 4 is the circuit structure diagram of the second detection circuit provided by the embodiment of the present utility model;

[0030] Figure 5 is the circuit structure diagram of the adjustment and calibration circuit provided by the embodiment of the present utility model;

[0031] Figure 6 It is the circuit structure diagram of the signal acquisition circuit provided by the embodiment of the present utility model;

[0032] Figure 7 It is the circuit structure diagram of the power supply circuit provided by the embodiment of the present utility model;

[0033] The reference signs are as follows:

[0034] 10 - Control detection circuit, 20 - First detection circuit, 30 - Second detection circuit, 40 - Adjustment and calibration circuit, 50 - Signal acquisition circuit, 60 - Power supply circuit. Specific embodiments

[0035] Next, the solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0036] It should be noted that if there are directional indications (such as up, down, left, right, front, back,...) involved in the embodiments of the present utility model, the directional indications are only used to explain the relative position relationship and movement conditions between components in a specific posture (as shown in the drawings). If the specific posture changes, the directional indications will also change accordingly.

[0037] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present utility model, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present utility model.

[0038] Please refer to Figure 1 , which is the structural block diagram of the immunofluorescence dual - light - source calibration control system provided by the embodiment of the present utility model.

[0039] The present utility model provides an immunofluorescence dual-light source calibration control system, which includes a control and detection circuit 10, a first detection circuit 20, a second detection circuit 30, an adjustment and calibration circuit 40, and a signal acquisition circuit 50. The first detection circuit 20 and the second detection circuit 30 are both electrically connected to the signal acquisition circuit 50. The first detection circuit 20 or the second detection circuit 30 is electrically connected to the adjustment and calibration circuit 40. The control and detection circuit 10 is electrically connected to the adjustment and calibration circuit 40 and the signal acquisition circuit 50 respectively.

[0040] Specifically, after connecting the immunofluorescence dual-light source calibration control system to devices such as a fluorescence microscope and a fluorescence analyzer required for immunofluorescence detection, before entering the formal immunofluorescence detection stage, start the automatic calibration mechanism of the immunofluorescence dual-light source calibration control system. The signal acquisition circuit 50 respectively collects the detection signals of the first detection circuit 20 and the second detection circuit 30, such as voltage values, and continuously sends them to the control and detection circuit 10. The control and detection circuit 10 compares the magnitudes of the voltage values received from the first detection circuit 20 and the second detection circuit 30. If it is confirmed that the magnitudes of the two values are not equal, the adjustment and calibration circuit 40 is triggered. After being triggered by the control and detection circuit 10, the adjustment and calibration circuit 40 performs adjustment and calibration according to the adjustment signal sent by the control and detection circuit 10 to change the voltage value of the first detection circuit 20 or the second detection circuit 30 (if the adjustment and calibration circuit 40 is connected to the first detection circuit 20, the voltage value of the first detection circuit 20 is adjusted and calibrated. Specifically, when the voltage value of the first detection circuit 20 is greater than the voltage value of the second detection circuit 30, the voltage value of the first detection circuit 20 is decreased; when the voltage value of the first detection circuit 20 is less than the voltage value of the second detection circuit 30, the voltage value of the first detection circuit 20 is increased. If the adjustment and calibration circuit 40 is connected to the second detection circuit 30, the voltage value of the second detection circuit 30 is adjusted and calibrated. Specifically, when the voltage value of the second detection circuit 30 is greater than the voltage value of the first detection circuit 20, the voltage value of the second detection circuit 30 is decreased; when the voltage value of the second detection circuit 30 is less than the voltage value of the first detection circuit 20, the voltage value of the second detection circuit 30 is increased), until the voltage values of the two detection circuits are exactly equal, then the automatic calibration process of the immunofluorescence dual-light source calibration control system is completed. After the automatic calibration process of the immunofluorescence dual-light source calibration control system is completed, it can ensure that the intensity and wavelength of the light source remain stable within a certain range, eliminate the changes in light source parameters and instrument drift, and improve the accuracy and reliability of immunofluorescence detection results. This automatic calibration process can be carried out before each immunofluorescence detection or regularly to further ensure the stability of immunofluorescence detection results.

[0041] It should be noted that the detection signals collected by the signal acquisition circuit 50 can be voltage values, current values, or other types of analog signals, or digital signals. Correspondingly, the adjustment and calibration circuit 40 can select a potentiometer to adjust the voltage value or current value, or other components such as adjustable capacitors and adjustable inductors can be used to achieve the adjustment and calibration of the detection signals. The specific type of detection signal and the type of adjustment component can be determined according to the design of the immunofluorescence dual-light source calibration control system and the required signal processing and analysis methods. No further examples will be given here. As long as the immunofluorescence dual-light source calibration control system can finally achieve the automatic calibration of the immunofluorescence dual-light source.

[0042] In the immunofluorescence dual-light source calibration control system provided in this embodiment, the signal acquisition circuit 50 is responsible for collecting the detection signals of the first detection circuit 20 and the second detection circuit 30 and sending them to the control detection circuit 10; when the control detection circuit 10 confirms that the numerical values of the detection signals of the first detection circuit 20 and the second detection circuit 30 are not equal, the adjustment and calibration circuit 40 is triggered to change the numerical value of the detection signal of the first detection circuit 20 or the second detection circuit 30 until the numerical values of the detection signals of the first detection circuit 20 and the second detection circuit 30 are exactly equal. Once it is reconfirmed that the numerical values of the detection signals of the two detection circuits are exactly equal, it means that the immunofluorescence dual-light source calibration control system has completed the automatic calibration process and can enter the formal immunofluorescence detection stage. The entire calibration process depends on the collaborative cooperation of the control detection circuit 10, the first detection circuit 20, the second detection circuit 30, the adjustment and calibration circuit 40, and the signal acquisition circuit 50, and can achieve the automatic calibration of the immunofluorescence dual-light source for detection. After the automatic calibration is completed using this immunofluorescence dual-light source calibration control system, it can effectively eliminate the influence of changes in light source intensity and wavelength and the drift of instrument parameters on the accuracy of the detection results, thereby improving the accuracy and reliability of the immunofluorescence detection results and providing more reliable data support for medical diagnosis and biomedical research.

[0043] Please refer to Figure 2 , which is the circuit structure diagram of the control detection circuit 10 provided in the embodiment of the present invention.

[0044] In some embodiments of the present application, the control detection circuit 10 includes an MCU control chip U5 of the GD32F130F8P6 type, a resistor R29, a resistor R26, a capacitor C19, a capacitor C11, a resistor R11, a resistor R32, a three-pin connector J2, a resistor R27, a resistor R28, a resistor R22, a resistor R34, an NPN-type phototransistor Q2, an NPN-type phototransistor Q3, a light-emitting diode D2, a light-emitting diode D4, and a resistor R25;

[0045] Among them, the first pin of the MCU control chip U5 is grounded through the resistor R29. The second, third, sixth, and ninth pins of the MCU control chip U5 are all floating. The fourth pin of the MCU control chip U5 is respectively connected to one end of the resistor R26 and one end of the capacitor C19. The fifth pin of the MCU control chip U5 is respectively connected to the 3.3V power supply terminal, the other end of the resistor R26, and one end of the capacitor C11. The seventh pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R11. The eighth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R32. The tenth pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q3 through the resistor R34. The eleventh pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q2 through the resistor R22. The twelfth pin of the MCU control chip U5 is connected to the MCP A0 terminal. The thirteenth pin of the MCU control chip U5 is connected to the MCP SCL terminal. The fourteenth pin of the MCU control chip U5 is connected to the MCP SDA terminal. The fifteenth pin of the MCU control chip U5 is grounded. The sixteenth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal. The seventeenth pin of the MCU control chip U5 is connected to the TX signal sending terminal through the resistor R28. The eighteenth pin of the MCU control chip U5 is connected to the RX signal receiving terminal through the resistor R27. The nineteenth pin of the MCU control chip U5 is connected to the first end of the three-pin connector J2. The twentieth pin of the MCU control chip U5 is connected to the second end of the three-pin connector J2;

[0046] The other ends of the capacitor C11 and the capacitor C19 are both grounded. The third end of the three-pin connector J2 is grounded. The emitters of the NPN phototransistor Q2 and the NPN phototransistor Q3 are both grounded. The collector of the NPN phototransistor Q2 is connected to the negative electrode of the light-emitting diode D2. The collector of the NPN phototransistor Q3 is connected to the negative electrode of the light-emitting diode D4. The positive electrodes of the light-emitting diode D2 and the light-emitting diode D4 are both connected to one end of the resistor R25. The other end of the resistor R25 is connected to the VCC power supply terminal.

[0047] Specifically, the MCU control chip of the GD32F130F8P6 type is a 32-bit ARM Cortex-M3 core microcontroller (MCU) control chip with a running frequency up to 72 MHz, having 64 KB of Flash memory and 8 KB of SRAM, as well as rich peripheral functions including timers, ADC, DAC, UART, SPI, I2C, etc. It also supports multiple clock sources and communication interfaces, has low-power modes and multiple power management functions, and security features such as hardware CRC check and independent watchdog, providing reliability and stability for the system.

[0048] In some embodiments of the present application, the light-emitting diode D2 is a 365-nm type light-emitting diode, and the light-emitting diode D4 is a 410-nm type light-emitting diode.

[0049] Specifically, in immunofluorescence detection, by using a dual light source (generally a dual light source of 365 nm and 410 nm) for detection, different types of fluorescent dyes can be excited simultaneously, thereby realizing the detection and analysis of multiple targets. Immunofluorescence detection usually uses antibodies or antigens labeled with fluorescent dyes to label the targets, and different fluorescent dyes have optimal excitation and emission characteristics for different wavelengths. Among them, the 365-nm wavelength is usually used to excite common fluorescent dyes such as FITC (fluorescein isothiocyanate) and fluorescein, etc., while the 410-nm wavelength is more suitable for exciting another type of fluorescent dyes such as R-PE (R-phycoerythrin), etc. This dual-wavelength design can improve the sensitivity and accuracy of detection, and at the same time reduce errors, providing reliable data support for scientific research and clinical diagnosis.

[0050] Please refer to Figure 3 , which is the circuit structure diagram of the first detection circuit 20 provided by the embodiment of the present utility model.

[0051] In some embodiments of the present application, the first detection circuit 20 includes an operational amplifier chip U1 of the AD822AR type, a light-emitting diode D1, a capacitor C1, a resistor R3, a resistor R6, a resistor R12, a capacitor C2, a resistor R4, a resistor R1, a resistor R2, a resistor R10, a resistor R31, a variable potentiometer VR1, a resistor R5, a capacitor C3, and a resistor R9;

[0052] Among them, the first pin of the operational amplifier chip U1 is connected to one end of the resistor R4, the second pin of the operational amplifier chip U1 is connected to one end of the resistor R1, the third pin of the operational amplifier chip U1 is connected to one end of the resistor R2, the fourth pin of the operational amplifier chip U1 is respectively connected to one end of the resistor R9 and the ground terminal, the fifth pin of the operational amplifier chip U1 is connected to one end of the resistor R5, the sixth pin of the operational amplifier chip U1 is connected to the other end of the resistor R9, the seventh pin of the operational amplifier chip U1 is connected to the 0.8 - 1.2V voltage signal terminal, and the eighth pin of the operational amplifier chip U1 is respectively connected to one end of the capacitor C3 and the 3.3V power supply terminal;

[0053] The other end of the resistor R4 is respectively connected to one end of the capacitor C1, one end of the resistor R3, and the other end of the resistor R5. The other end of the resistor R1 is respectively connected to the other end of the resistor R3, the other end of the capacitor C1, and the negative electrode of the light - emitting diode D1. The other end of the resistor R2 is respectively connected to one end of the resistor R10, one end of the capacitor C2, and one end of the resistor R6. The other end of the resistor R6 is respectively connected to the positive electrode of the light - emitting diode D1 and one end of the resistor R12. The other end of the resistor R12 and the other end of the capacitor C2 are both grounded. The other end of the resistor R10 is connected to one end of the adjustable potentiometer VR1 and the adjustment terminal of the adjustable potentiometer VR1 through the resistor R31. The other end of the adjustable potentiometer VR1 is grounded, and the other end of the capacitor C3 is grounded.

[0054] Specifically, the operational amplifier chip of the AD822AR type is a dual - channel, low - power, high - precision operational amplifier chip. It has low - noise, high - precision amplification and output characteristics, is suitable for applications with high requirements for signal quality, and provides accurate signal amplification and processing capabilities for the system. This chip has a wide input voltage range, good common - mode rejection ratio and offset voltage characteristics, and can process larger input signals. At the same time, it adopts a low - power design, is suitable for applications with high requirements for power consumption, and can work under single - power or dual - power supply conditions.

[0055] Please refer to Figure 4 , which is the circuit structure diagram of the second detection circuit 30 provided by the embodiment of the present invention.

[0056] In some embodiments of the present application, the second detection circuit 30 includes an operational amplifier chip U2 of the AD822AR type, a light - emitting diode D3, a resistor R24, a resistor R30, a resistor R17, a capacitor C4, a resistor R18, a resistor R19, a resistor R20, a resistor R16, a capacitor C5, a resistor R23, and a resistor R33;

[0057] Among them, the first pin of the operational amplifier chip U2 is connected to one end of the resistor R18, the second pin of the operational amplifier chip U2 is connected to one end of the resistor R19, the third pin of the operational amplifier chip U2 is connected to one end of the resistor R20, the fourth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R23 and the ground terminal, the fifth pin of the operational amplifier chip U2 is connected to one end of the resistor R16, the sixth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R33 and the other end of the resistor R23, the seventh pin of the operational amplifier chip U2 is respectively connected to the other end of the resistor R33 and the 0.8 - 1.2V voltage signal terminal, and the eighth pin of the operational amplifier chip U2 is respectively connected to one end of the capacitor C5 and the 3.3V power supply terminal;

[0058] The other end of the resistor R18 is respectively connected to one end of the capacitor C4, the other end of the resistor R16, and one end of the resistor R17. The other end of the resistor R19 is respectively connected to the other end of the resistor R17, the other end of the capacitor C4, and the negative electrode of the light-emitting diode D3. The other end of the resistor R20 is respectively connected to the positive electrode of the light-emitting diode D3 and one end of the resistor R24. The other end of the resistor R24 is grounded through the resistor R30, and the other end of the capacitor C5 is grounded.

[0059] Specifically, the circuit structures of the first detection circuit 20 and the second detection circuit 30 are basically the same to ensure that they have similar responses and characteristics during the automatic calibration process, avoid introducing inconsistencies and instabilities, and improve the consistency and repeatability of the system. By adopting basically the same circuit structure, the first detection circuit 20 and the second detection circuit 30 can work better together and improve the calibration accuracy. Additionally, considering various factors such as circuit sensitivity, noise level, and compatibility with the system, the voltage signal value range of the first detection circuit 20 and the second detection circuit 30 is 0.8 - 1.2V.

[0060] Please refer to Figure 5 which is the circuit structure diagram of the adjustment and calibration circuit 40 provided by the embodiment of the present invention.

[0061] In some embodiments of the present application, the adjustment and calibration circuit 40 is electrically connected to the first detection circuit 20.

[0062] Specifically, the adjustment and calibration circuit 40 can be electrically connected to the first detection circuit 20 or the second detection circuit 30, and only one of them needs to be connected. In the actual application process, the adjustment and calibration circuit 40 generally will not be electrically connected to both the first detection circuit 20 and the second detection circuit 30 at the same time to avoid increasing the complexity of adjustment and calibration.

[0063] In some embodiments of the present application, the adjustment and calibration circuit 40 includes a digital potentiometer chip U6 of the MCP4561-503E / MS type and a resistor R8;

[0064] Among them, the first pin of the digital potentiometer chip U6 is connected to the MCP A0 terminal, the second pin of the digital potentiometer chip U6 is connected to the MCP SCL terminal, the third pin of the digital potentiometer chip U6 is connected to the MCP SDA terminal, the fourth pin of the digital potentiometer chip U6 is grounded, the fifth pin of the digital potentiometer chip U6 is connected to one end of the resistor R8, the sixth pin of the digital potentiometer chip U6 is respectively connected to the other end of the resistor R8 and the 0.8-1.2V voltage signal terminal, the seventh pin of the digital potentiometer chip U6 is left floating, and the eighth pin of the digital potentiometer chip U6 is connected to the 3.3V power supply terminal.

[0065] Specifically, the digital potentiometer chip of the MCP4561-503E / MS type has a resistance range of 50 kΩ and 256 resistance stages, and can provide precise resistance adjustment, thereby improving the accuracy and stability of the system. This digital potentiometer chip is programmable and can be programmed through a serial interface, and can dynamically adjust the resistance value according to needs, realizing flexible control of the calibration circuit. In addition, this digital potentiometer chip has stable characteristics and can maintain consistent performance during long-term use, ensuring the reliability and long-term stability of the adjustment and calibration circuit 40.

[0066] Please refer to Figure 6 , which is the circuit structure diagram of the signal acquisition circuit 50 provided by the embodiment of the present utility model.

[0067] In some embodiments of the present application, the signal acquisition circuit 50 includes a 24-bit analog-to-digital converter chip U4 of the HY3116 type, a resistor R7, a capacitor C17, a capacitor C14, a resistor R21, a resistor R13, a capacitor C16, a capacitor C6, and a capacitor C15;

[0068] Among them, the first pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C14 and the 3.3V power supply terminal, the second pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C14 and the ground terminal, the third pin of the 24-bit analog-to-digital converter chip U4 is connected to one end of the resistor R21, the fourth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the resistor R13 and one end of the capacitor C16, the fifth and sixth pins of the 24-bit analog-to-digital converter chip U4 are both connected to one end of the capacitor C15, the seventh pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C15 and the ground terminal, the eighth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SDA terminal, the ninth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SCL terminal, and the tenth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C6 and the 3.3V power supply terminal;

[0069] The other end of the resistor R13 is connected to the 0.8 - 1.2V voltage signal terminal, the other ends of the capacitor C16 and the capacitor C6 are both grounded, the other end of the resistor R21 is respectively connected to one end of the capacitor C17 and one end of the resistor R7, the other end of the capacitor C17 is grounded, and one end of the resistor R7 is connected to the 0.8 - 1.2V voltage signal terminal.

[0070] Specifically, the 24-bit analog-to-digital converter chip of the HY3116 type is a 24-bit ADC chip, which can provide up to 2^24 = 16777216 different digital output levels, thus achieving extremely high conversion accuracy and being suitable for application scenarios that require high-resolution signal conversion. It supports differential input and single-ended input, has the characteristics of low noise performance and high precision, and is very suitable for the field of medical equipment.

[0071] Please refer to Figure 7 , which is the circuit structure diagram of the power supply circuit 60 provided by the embodiment of the present invention.

[0072] In some embodiments of the present application, the immunofluorescence dual-light source calibration control system further includes a power supply circuit 60, and the power supply circuit 60 is electrically connected to the control detection circuit 10.

[0073] In some embodiments of the present application, the power supply circuit 60 includes a low-dropout voltage regulator chip U3 of the TLV70233DBVT type, a capacitor C9, a resistor R15, and a capacitor C10;

[0074] Among them, the first pin of the low-dropout regulator chip U3 is respectively connected to one end of the capacitor C9 and the power supply terminal, the second pin of the low-dropout regulator chip U3 is respectively connected to the other end of the capacitor C9 and the ground terminal, the third pin of the low-dropout regulator chip U3 is connected to the power supply terminal, the fourth pin of the low-dropout regulator chip U3 is left floating, the fifth pin of the low-dropout regulator chip U3 is respectively connected to one end of the capacitor C10, one end of the resistor R15 and the 3.3V power supply terminal, the other end of the capacitor C10 is grounded, and the other end of the resistor R15 is connected to the 3.3V power supply terminal.

[0075] Specifically, the TLV70233DBVT type low-dropout regulator chip is a high-performance, low-power positive regulator with a working voltage range of 2.5V to 5.5V and an output voltage of 3.3V. This low-dropout regulator chip has ultra-low quiescent current and extremely low dropout voltage, and is suitable for occasions with strict power consumption requirements such as portable electronic devices and battery-powered systems. This low-dropout regulator chip adopts a miniature package, is suitable for application scenarios with limited space, and has thermal shutdown and short-circuit protection functions, capable of providing a stable and reliable power output.

[0076] It should be noted that after automatic calibration using the immunofluorescence dual-light source calibration control system provided by the present invention, immunofluorescence dual-light source detection can be performed. The specific process of this immunofluorescence dual-light source detection is as follows: at the start of detection, control the detection circuit 10 to scan the two-dimensional code on the reagent card to obtain the identity information of the reagent card; control the detection circuit 10 to automatically turn on the light sources of corresponding wavelengths according to the identity information of the reagent card, such as the light source of 365nm and the light source of 410nm; after the light emitted by the light source irradiates the reagent card, the light-emitting diode receives the fluorescence signal reflected by the reagent card for subsequent analysis of the fluorescence signal, so as to complete the detection and analysis of the target substance on the reagent card.

[0077] The immunofluorescence dual-light source calibration control system provided by the embodiment of the present invention can ensure that the system still maintains accurate and reliable performance after long-term use by automatically calibrating the immunofluorescence dual-light source regularly or before each detection. The automatic calibration of the immunofluorescence dual-light source is a key step in immunofluorescence dual-light source detection, which can eliminate the drift and attenuation of the light source, and ensure the stability and consistency of the light source. Through automatic calibration, the system can provide accurate fluorescence signals, thus ensuring the accuracy and reliability of immunofluorescence detection. In addition, regular automatic calibration can also detect and correct some potential problems to ensure that the system is always in the best working state.

[0078] It should be noted that the technical solutions between the various embodiments of the present utility model can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0079] The above are only partial or preferred embodiments of the present utility model. Whether in terms of text or drawings, the scope of protection of the present utility model cannot be limited thereby. Any equivalent structural transformation made by using the content of the specification and drawings of the present utility model under the overall concept of the present utility model, or any direct / indirect application in other related technical fields is included within the scope of protection of the present utility model.

Claims

1. An immunofluorescence dual-light-source calibration control system, characterized in that, It includes a control detection circuit, a first detection circuit, a second detection circuit, an adjustment and calibration circuit, and a signal acquisition circuit. The first detection circuit and the second detection circuit are both electrically connected to the signal acquisition circuit. The first detection circuit or the second detection circuit is electrically connected to the adjustment and calibration circuit. The control detection circuit is respectively electrically connected to the adjustment and calibration circuit and the signal acquisition circuit.

2. The immunofluorescence dual-light source calibration control system according to claim 1, wherein The control detection circuit includes an MCU control chip U5 of the GD32F130F8P6 type, a resistor R29, a resistor R26, a capacitor C19, a capacitor C11, a resistor R11, a resistor R32, a three-pin connector J2, a resistor R27, a resistor R28, a resistor R22, a resistor R34, an NPN phototransistor Q2, an NPN phototransistor Q3, a light-emitting diode D2, a light-emitting diode D4, and a resistor R25; Among them, the first pin of the MCU control chip U5 is grounded through the resistor R29. The second pin, the third pin, the sixth pin, and the ninth pin of the MCU control chip U5 are all floating. The fourth pin of the MCU control chip U5 is respectively connected to one end of the resistor R26 and one end of the capacitor C19. The fifth pin of the MCU control chip U5 is respectively connected to the 3.3V power supply terminal, the other end of the resistor R26, and one end of the capacitor C11. The seventh pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R11. The eighth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal through the resistor R32. The tenth pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q3 through the resistor R34. The eleventh pin of the MCU control chip U5 is connected to the base of the NPN phototransistor Q2 through the resistor R22. The twelfth pin of the MCU control chip U5 is connected to the MCP A0 terminal. The thirteenth pin of the MCU control chip U5 is connected to the MCP SCL terminal. The fourteenth pin of the MCU control chip U5 is connected to the MCP SDA terminal. The fifteenth pin of the MCU control chip U5 is grounded. The sixteenth pin of the MCU control chip U5 is connected to the 3.3V power supply terminal. The seventeenth pin of the MCU control chip U5 is connected to the TX signal sending terminal through the resistor R28. The eighteenth pin of the MCU control chip U5 is connected to the RX signal receiving terminal through the resistor R27. The nineteenth pin of the MCU control chip U5 is connected to the first end of the three-pin connector J2. The twentieth pin of the MCU control chip U5 is connected to the second end of the three-pin connector J2; The other end of the capacitor C11 and the other end of the capacitor C19 are both grounded. The third terminal of the three-pin connector J2 is grounded. The emitters of the NPN phototransistors Q2 and Q3 are both grounded. The collector of the NPN phototransistor Q2 is connected to the negative electrode of the light-emitting diode D2. The collector of the NPN phototransistor Q3 is connected to the negative electrode of the light-emitting diode D4. The positive electrodes of the light-emitting diode D2 and the light-emitting diode D4 are both connected to one end of the resistor R25. The other end of the resistor R25 is connected to the VCC power supply terminal.

3. The immunofluorescence dual-light source calibration control system according to claim 2, wherein The light-emitting diode D2 is a light-emitting diode of the 365nm type, and the light-emitting diode D4 is a light-emitting diode of the 410nm type.

4. The immunofluorescence dual-light source calibration control system according to claim 1, wherein The first detection circuit includes an operational amplifier chip U1 of the AD822AR type, a light-emitting diode D1, a capacitor C1, a resistor R3, a resistor R6, a resistor R12, a capacitor C2, a resistor R4, a resistor R1, a resistor R2, a resistor R10, a resistor R31, a variable potentiometer VR1, a resistor R5, a capacitor C3, and a resistor R9; Among them, the first pin of the operational amplifier chip U1 is connected to one end of the resistor R4. The second pin of the operational amplifier chip U1 is connected to one end of the resistor R1. The third pin of the operational amplifier chip U1 is connected to one end of the resistor R2. The fourth pin of the operational amplifier chip U1 is respectively connected to one end of the resistor R9 and the ground terminal. The fifth pin of the operational amplifier chip U1 is connected to one end of the resistor R5. The sixth pin of the operational amplifier chip U1 is connected to the other end of the resistor R9. The seventh pin of the operational amplifier chip U1 is connected to the 0.8 - 1.2V voltage signal terminal. The eighth pin of the operational amplifier chip U1 is respectively connected to one end of the capacitor C3 and the 3.3V power supply terminal; The other end of the resistor R4 is respectively connected to one end of the capacitor C1, one end of the resistor R3, and the other end of the resistor R5. The other end of the resistor R1 is respectively connected to the other end of the resistor R3, the other end of the capacitor C1, and the negative electrode of the light-emitting diode D1. The other end of the resistor R2 is respectively connected to one end of the resistor R10, one end of the capacitor C2, and one end of the resistor R6. The other end of the resistor R6 is respectively connected to the positive electrode of the light-emitting diode D1 and one end of the resistor R12. The other end of the resistor R12 and the other end of the capacitor C2 are both grounded. The other end of the resistor R10 is connected to one end of the variable potentiometer VR1 and the adjustment terminal of the variable potentiometer VR1 through the resistor R31. The other end of the variable potentiometer VR1 is grounded. The other end of the capacitor C3 is grounded.

5. The immunofluorescence dual-light-source calibration control system according to claim 1, wherein The second detection circuit includes an operational amplifier chip U2 of the AD822AR type, a light-emitting diode D3, a resistor R24, a resistor R30, a resistor R17, a capacitor C4, a resistor R18, a resistor R19, a resistor R20, a resistor R16, a capacitor C5, a resistor R23, and a resistor R33; Among them, the first pin of the operational amplifier chip U2 is connected to one end of the resistor R18, the second pin of the operational amplifier chip U2 is connected to one end of the resistor R19, the third pin of the operational amplifier chip U2 is connected to one end of the resistor R20, the fourth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R23 and the ground terminal, the fifth pin of the operational amplifier chip U2 is connected to one end of the resistor R16, the sixth pin of the operational amplifier chip U2 is respectively connected to one end of the resistor R33 and the other end of the resistor R23, the seventh pin of the operational amplifier chip U2 is respectively connected to the other end of the resistor R33 and the 0.8 - 1.2V voltage signal terminal, and the eighth pin of the operational amplifier chip U2 is respectively connected to one end of the capacitor C5 and the 3.3V power supply terminal; The other end of the resistor R18 is respectively connected to one end of the capacitor C4, the other end of the resistor R16 and one end of the resistor R17, the other end of the resistor R19 is respectively connected to the other end of the resistor R17, the other end of the capacitor C4 and the negative electrode of the light-emitting diode D3, the other end of the resistor R20 is respectively connected to the positive electrode of the light-emitting diode D3 and one end of the resistor R24, the other end of the resistor R24 is grounded through the resistor R30, and the other end of the capacitor C5 is grounded.

6. The immunofluorescence dual-light-source calibration control system according to claim 1, wherein The adjustment and calibration circuit is electrically connected to the first detection circuit.

7. The immunofluorescence dual-light-source calibration control system according to claim 6, wherein The adjustment and calibration circuit includes a digital potentiometer chip U6 of the MCP4561 - 503E / MS type and a resistor R8; Among them, the first pin of the digital potentiometer chip U6 is connected to the MCP A0 terminal, the second pin of the digital potentiometer chip U6 is connected to the MCP SCL terminal, the third pin of the digital potentiometer chip U6 is connected to the MCP SDA terminal, the fourth pin of the digital potentiometer chip U6 is grounded, the fifth pin of the digital potentiometer chip U6 is connected to one end of the resistor R8, the sixth pin of the digital potentiometer chip U6 is respectively connected to the other end of the resistor R8 and the 0.8 - 1.2V voltage signal terminal, the seventh pin of the digital potentiometer chip U6 is left floating, and the eighth pin of the digital potentiometer chip U6 is connected to the 3.3V power supply terminal.

8. The immunofluorescence dual-light-source calibration control system according to claim 1, wherein The signal acquisition circuit includes a 24 - bit analog - to - digital converter chip U4 of the HY3116 type, a resistor R7, a capacitor C17, a capacitor C14, a resistor R21, a resistor R13, a capacitor C16, a capacitor C6 and a capacitor C15; Among them, the first pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C14 and the 3.3V power supply terminal, the second pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C14 and the ground terminal, the third pin of the 24-bit analog-to-digital converter chip U4 is connected to one end of the resistor R21, the fourth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the resistor R13 and one end of the capacitor C16, the fifth and sixth pins of the 24-bit analog-to-digital converter chip U4 are both connected to one end of the capacitor C15, the seventh pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to the other end of the capacitor C15 and the ground terminal, the eighth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SDA terminal, the ninth pin of the 24-bit analog-to-digital converter chip U4 is connected to the SCL terminal, and the tenth pin of the 24-bit analog-to-digital converter chip U4 is respectively connected to one end of the capacitor C6 and the 3.3V power supply terminal; The other end of the resistor R13 is connected to the 0.8 - 1.2V voltage signal terminal, the other ends of the capacitor C16 and the capacitor C6 are both grounded, the other end of the resistor R21 is respectively connected to one end of the capacitor C17 and one end of the resistor R7, the other end of the capacitor C17 is grounded, and one end of the resistor R7 is connected to the 0.8 - 1.2V voltage signal terminal.

9. The immunofluorescence dual-light-source calibration control system according to claim 1, wherein It further includes a power supply circuit, and the power supply circuit is electrically connected to the control and detection circuit.

10. The immunofluorescence dual-light source calibration control system according to claim 9, wherein The power supply circuit includes a low-dropout voltage regulator chip U3 of the TLV70233DBVT type, a capacitor C9, a resistor R15, and a capacitor C10; Among them, the first pin of the low-dropout voltage regulator chip U3 is respectively connected to one end of the capacitor C9 and the power supply terminal, the second pin of the low-dropout voltage regulator chip U3 is respectively connected to the other end of the capacitor C9 and the ground terminal, the third pin of the low-dropout voltage regulator chip U3 is connected to the power supply terminal, the fourth pin of the low-dropout voltage regulator chip U3 is left floating, the fifth pin of the low-dropout voltage regulator chip U3 is respectively connected to one end of the capacitor C10, one end of the resistor R15, and the 3.3V power supply terminal, the other end of the capacitor C10 is grounded, and the other end of the resistor R15 is connected to the 3.3V power supply terminal.

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