Extraction and concentration device for ion exchange resin based on protein purification

Abstract

The utility model relates to the technical field of protein purification and extraction, and discloses an extraction and concentration device based on protein purification ion exchange resin, which comprises a support seat, an ion exchange resin tank fixedly connected to the top of the support seat, and an elution device fixedly connected to the outer wall of one side of the ion exchange resin tank. The protein purification device is compact and reasonable in structural design, continuous operation from sample injection, ion exchange, elution to concentration is achieved through automatic control, the protein purification efficiency and purity are improved, the product quality is improved, and the product quality is improved. And continuous treatment is realized, the production efficiency is greatly improved, and the problems of limited purification efficiency and low automation degree of the extraction and concentration device for the ion exchange resin based on protein purification are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification and extraction, and specifically relates to an extraction and concentration device for protein purification ion exchange resin. Background Technique

[0002] As the main bearer of life activities, proteins play a crucial role in organisms. However, the proteins extracted from organisms often contain various impurities, such as other proteins, nucleic acids, polysaccharides, lipids, etc. The presence of these impurities will seriously affect the properties and functions of proteins. Therefore, purifying proteins is an important task in biological science research and industrial production. Ion exchange resin is a polymer material with exchangeable ion groups, which can exchange with ions in the solution. In protein purification, ion exchange resin uses its specific adsorption effect on the surface charge of proteins to achieve the separation and purification of proteins. By selecting appropriate ion exchange resins and operating conditions, impurities in protein samples can be efficiently removed, and the purity of proteins can be improved.

[0003] Traditional equipment has certain limitations in purification efficiency. Due to the limitations of equipment design and operating conditions, it is impossible to achieve efficient and rapid purification of target proteins, thus affecting the efficiency of the entire production process, and the degree of automation is low. For this reason, we propose an extraction and concentration device for protein purification ion exchange resin. Content of the Utility Model

[0004] (1) Technical Problems to be Solved

[0005] Aiming at the deficiencies of the prior art, the utility model provides an extraction and concentration device for protein purification ion exchange resin, which solves the above problems.

[0006] (2) Technical Solutions

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solution: An extraction and concentration device for protein purification ion exchange resin, including a support seat, a ion exchange resin tank is fixedly connected to the top of the support seat, an elution device is fixedly connected to the outer wall of one side of the ion exchange resin tank, a sample injection device is fixedly connected to the output end of the top of the elution device, and a concentration device is fixedly connected to the output end of the bottom of the ion exchange resin tank.

[0008] Preferably, the ion exchange resin tank includes a tank body, a blanking plate, an ion exchange column, a discharge cavity, and a discharge pipe. The support seat is fixedly connected to the tank body at the top, the blanking plate is fixedly connected to the inner wall of one end of the tank body, the ion exchange column is fixedly connected to the output end of the top of the blanking plate, a discharge cavity is arranged between the blanking plate and the bottom of the inner wall of the tank body, and the output end of the bottom of the tank body is fixedly connected to the discharge cavity.

[0009] Preferably, a connecting pipe is fixedly connected to the outer wall of one side of the tank body, and the connecting pipe penetrates through one side of the inner wall of the tank body. The output end of one side of the connecting pipe is located above the blanking plate. The output end of the connecting pipe far from the tank body is fixedly connected with an extraction pump, and the output end of the bottom of the extraction pump is fixedly connected with a discharge pipe.

[0010] Preferably, the elution device includes an eluent injection tank, an input pump, a second connecting pipe, a partition plate, and a spray head. An eluent injection tank is fixedly connected to the outer wall of the tank body on the side far from the connecting pipe. The output end of one side of the eluent injection tank is fixedly connected with an input pump. The output end of the input pump far from the eluent injection tank is fixedly connected with a second connecting pipe, and the second connecting pipe penetrates through one side of the inner wall of the tank body. A partition plate is fixedly connected to the inner wall of the tank body at the end far from the blanking plate. The output end of the bottom of the partition plate is fixedly connected with a spray head, and the output end of the second connecting pipe far from the input pump is located above the partition plate.

[0011] Preferably, the sample injection device includes a blanking pipe, a second input pump, and a material pipe. The output end of the top of the tank body is fixedly connected with a blanking pipe, and the blanking pipe penetrates through the top of the inner wall of the tank body. The output end of the bottom of the blanking pipe penetrates through the bottom of the partition plate. The output end of the top of the blanking pipe is fixedly connected with a second input pump, and the output end of the second input pump far from the blanking pipe is fixedly connected with a material pipe.

[0012] Preferably, the concentration device includes a bottom plate, a support plate, a pressure pump, a discharge tank, an ultrafiltration tank, a second ultrafiltration tank, a connecting pipe, a third input pump, a third connecting pipe, a shunt pipe, a fourth connecting pipe, a fifth connecting pipe, a confluence pipe, and a discharge valve; the output end of the discharge pipe away from the tank body is fixedly connected with a connecting pipe, the output end of the connecting pipe away from the discharge pipe is fixedly connected with a third input pump, the bottom of the third input pump is fixedly connected with a bottom plate, the top of the bottom plate away from the third input pump is fixedly connected with a support plate, one side outer wall of the support plate is fixedly connected with a pressure pump, one side outer wall of the support plate close to the pressure pump is fixedly connected with a discharge tank, one end outer wall of the support plate away from the pressure pump is fixedly connected with an ultrafiltration tank, one side outer wall of the support plate close to the ultrafiltration tank is fixedly connected with a second ultrafiltration tank, the output end of the third input pump away from the connecting pipe is fixedly connected with a third connecting pipe, the output end of the third connecting pipe away from the third input pump is fixedly connected to the pressure pump, the output end of the pressure pump away from the third connecting pipe is fixedly connected with a shunt pipe, the output ends of the shunt pipe away from the pressure pump are respectively fixedly connected to the top output ends of the ultrafiltration tank and the second ultrafiltration tank, the bottom output end of the ultrafiltration tank is fixedly connected with a fourth connecting pipe, the bottom output end of the second ultrafiltration tank is fixedly connected with a fifth connecting pipe, and one output end of the fourth connecting pipe and the fifth connecting pipe is fixedly connected to the confluence pipe, the output end of the confluence pipe away from the fourth connecting pipe and the fifth connecting pipe is fixedly connected to the bottom output end of the discharge tank, and the output end of the discharge tank close to the confluence pipe is fixedly connected with a discharge valve.

[0013] Preferably, there are multiple groups of the spray heads and the ion exchange columns.

[0014] (III) Beneficial effects

[0015] Compared with the prior art, the present invention provides an extraction and concentration device based on ion exchange resin for protein purification, which has the following beneficial effects:

[0016] 1. For the extraction and concentration device based on ion exchange resin for protein purification, through the specific adsorption of the ion exchange resin, impurities in the protein sample can be efficiently removed, and the purity of the protein can be improved. The selectivity of the ion exchange resin enables only the target protein or specific types of proteins to be retained, while other impurities are effectively removed.

[0017] 2. For the extraction and concentration device based on ion exchange resin for protein purification, an automated control system is adopted, and automated operations are realized in each link from sample injection, ion exchange, elution to concentration, reducing manual intervention, improving the accuracy and efficiency of operations. Automated operations also reduce the labor intensity and improve the comfort of the working environment.

[0018] 3. The extraction and concentration device for protein purification ion exchange resin can realize continuous purification of a large number of protein samples, which greatly improves production efficiency and meets the requirements of protein purification in industrial production. Description of the Drawings

[0019] Figure 1 It is a schematic structural diagram of the present utility model;

[0020] Figure 2 It is a bottom view schematic diagram of the present utility model;

[0021] Figure 3 It is a sectional view schematic diagram of the ion exchange resin tank of the present utility model;

[0022] Figure 4 It is a schematic diagram of the concentration device of the present utility model;

[0023] Figure 5 It is a side view schematic diagram of the concentration device of the present utility model.

[0024] In the figure, 1 is a support base; 2 is an ion exchange resin tank; 3 is an elution device; 4 is a sample injection device; 5 is a concentration device; 6 is a connecting pipe; 7 is a suction pump; 8 is a discharge pipe; 9 is an eluent injection tank; 10 is an input pump; 11 is a second connecting pipe; 12 is a partition board; 13 is a spray head; 14 is a tank body; 15 is a blanking plate; 16 is an ion exchange column; 17 is a discharge cavity; 18 is a discharge pipe; 19 is a blanking pipe; 20 is a second input pump; 21 is a material pipe; 22 is a bottom plate; 23 is a support plate; 24 is a pressure pump; 25 is a discharge tank; 26 is an ultrafiltration tank; 27 is a second ultrafiltration tank; 28 is a communicating pipe; 29 is a third input pump; 30 is a third connecting pipe; 31 is a shunt pipe; 32 is a fourth connecting pipe; 33 is a fifth connecting pipe; 34 is a confluence pipe; 35 is a discharge valve. Detailed Embodiments

[0025] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

[0026] Please refer to Figures 1-5 , an extraction and concentration device for protein purification ion exchange resin, includes a support base 1, an ion exchange resin tank 2 is fixedly connected to the top of the support base 1, an elution device 3 is fixedly connected to the outer wall of one side of the ion exchange resin tank 2, a sample injection device 4 is fixedly connected to the output end of the top of the elution device 3, and a concentration device 5 is fixedly connected to the output end of the bottom of the ion exchange resin tank 2.

[0027] Furthermore, the ion exchange resin tank 2 includes a tank body 14, a blanking plate 15, an ion exchange column 16, a discharge chamber 17, and a discharge pipe 18. The top of the support base 1 is fixedly connected to the tank body 14. One end inner wall of the tank body 14 is fixedly connected to the blanking plate 15. The top output end of the blanking plate 15 is fixedly connected to the ion exchange column 16. There is a discharge chamber 17 between the blanking plate 15 and the bottom of the inner wall of the tank body 14. The bottom output end of the tank body 14 is fixedly connected to the discharge chamber 17.

[0028] Furthermore, one side outer wall of the tank body 14 is fixedly connected to a connecting pipe 6, and the connecting pipe 6 penetrates through one side of the inner wall of the tank body 14. The output end on one side of the connecting pipe 6 is located above the blanking plate 15. The output end on the side of the connecting pipe 6 away from the tank body 14 is fixedly connected to a suction pump 7. The bottom output end of the suction pump 7 is fixedly connected to a discharge pipe 8.

[0029] Furthermore, the elution device 3 includes an eluent injection tank 9, an input pump 10, a second connecting pipe 11, a partition plate 12, and a spray head 13. One side outer wall of the tank body 14 away from the connecting pipe 6 is fixedly connected to the eluent injection tank 9. The output end on one side of the eluent injection tank 9 is fixedly connected to the input pump 10. The output end on the side of the input pump 10 away from the eluent injection tank 9 is fixedly connected to the second connecting pipe 11, and the second connecting pipe 11 penetrates through one side of the inner wall of the tank body 14. One end inner wall of the tank body 14 away from the blanking plate 15 is fixedly connected to the partition plate 12. The bottom output end of the partition plate 12 is fixedly connected to the spray head 13, and the output end on the side of the second connecting pipe 11 away from the input pump 10 is located above the partition plate 12.

[0030] Furthermore, the sample injection device 4 includes a blanking pipe 19, a second input pump 20, and a material pipe 21. The top output end of the tank body 14 is fixedly connected to the blanking pipe 19, and the blanking pipe 19 penetrates through the top of the inner wall of the tank body 14. The bottom output end of the blanking pipe 19 penetrates through the bottom of the partition plate 12. The top output end of the blanking pipe 19 is fixedly connected to the second input pump 20. The output end on the side of the second input pump 20 away from the blanking pipe 19 is fixedly connected to the material pipe 21.

[0031] Further, the concentration device 5 includes a bottom plate 22, a support plate 23, a pressure pump 24, a discharge tank 25, an ultrafiltration tank 26, a second ultrafiltration tank 27, a connecting pipe 28, a second input pump 29, a third connecting pipe 30, a shunt pipe 31, a fourth connecting pipe 32, a fifth connecting pipe 33, a confluence pipe 34, and a discharge valve 35. The output end of the discharge pipe 18 away from the tank body 14 is fixedly connected to the connecting pipe 28. The output end of the connecting pipe 28 away from the discharge pipe 18 is fixedly connected to the second input pump 29. The bottom of the second input pump 29 is fixedly connected to the bottom plate 22. One side of the top of the bottom plate 22 away from the second input pump 29 is fixedly connected to the support plate 23. One outer wall of the support plate 23 is fixedly connected to the pressure pump 24. One outer wall of the support plate 23 close to the pressure pump 24 is fixedly connected to the discharge tank 25. One outer wall of the support plate 23 away from the pressure pump 24 is fixedly connected to the ultrafiltration tank 26. One outer wall of the support plate 23 close to the ultrafiltration tank 26 is fixedly connected to the second ultrafiltration tank 27. The output end of the second input pump 29 away from the connecting pipe 28 is fixedly connected to the third connecting pipe 30. The output end of the third connecting pipe 30 away from the second input pump 29 is fixedly connected to the pressure pump 24. The output end of the pressure pump 24 away from the third connecting pipe 30 is fixedly connected to the shunt pipe 31. The output end of the shunt pipe 31 away from the pressure pump 24 is respectively fixedly connected to the top output ends of the ultrafiltration tank 26 and the second ultrafiltration tank 27. The bottom output end of the ultrafiltration tank 26 is fixedly connected to the fourth connecting pipe 32. The bottom output end of the second ultrafiltration tank 27 is fixedly connected to the fifth connecting pipe 33. And one output end of the fourth connecting pipe 32 and the fifth connecting pipe 33 is fixedly connected to the confluence pipe 34. The output end of the confluence pipe 34 away from the fourth connecting pipe 32 and the fifth connecting pipe 33 is fixedly connected to the bottom output end of the discharge tank 25. The output end of the discharge tank 25 close to the confluence pipe 34 is fixedly connected to the discharge valve 35.

[0032] Furthermore, multiple groups of spray heads 13 and ion exchange columns 16 are provided.

[0033] Working principle: First, the protein sample to be purified is pumped into the ion exchange resin tank 2 through the sampling device 4, that is, the combination of the feed pipe 19, the second input pump 20 and the feed pipe 21. Under the action of the pump, the sample enters the tank body 14 through the feed pipe 19, passes through the bottom of the isolation plate 12, and enters the area where the ion exchange column 16 is located. In the ion exchange column 16, the protein in the sample is exchanged with the ions on the resin to achieve preliminary purification of the protein. The multiple groups of design of the ion exchange column 16 increase the contact area and improve the exchange efficiency. When the ion exchange is completed, elution is performed through the elution device 3. The eluent in the eluent injection box 9 enters the tank body 14 through the second connecting pipe 11 under the action of the input pump 10, and is evenly sprayed on the ion exchange column 16 by the spray head 13 to elute the protein bound to the resin. The multiple groups of spray heads 13 and ion exchange columns 16 The group design ensures the uniformity and efficiency of elution. The eluted protein solution enters the concentrator 5 through the discharge pipe 18. In the concentrator, the solution is first pumped into the pressure pump 24 for pressurization through the second input pump 29. The pressurized solution enters the ultrafiltration tank 26 and the second ultrafiltration tank 27 through the shunt pipe 31 for ultrafiltration treatment. The ultrafiltration process removes small molecular impurities in the solution and realizes the concentration of protein. The ultrafiltered protein solution enters the confluence pipe 34 from the ultrafiltration tank 26 and the second ultrafiltration tank 27 through the fourth connecting pipe 32 and the fifth connecting pipe 33, and then enters the discharge tank 25 for collection. Finally, by controlling the discharge valve 35, the concentrated protein solution can be discharged from the discharge tank 25. The function of the connecting pipe 6, the extraction pump 7, and the discharge pipe 8 is to extract the waste liquid in the tank body 14 through the connecting pipe 6 and the extraction pump 7 and finally discharge it from the discharge pipe 8.

[0034] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An extraction and concentration device for protein purification ion exchange resin, comprising a support base (1), characterized in that: A support base (1) is fixedly connected to the top of an ion exchange resin tank (2). An elution device (3) is fixedly connected to the outer wall of one side of the ion exchange resin tank (2). The output end at the top of the elution device (3) is fixedly connected to a sample injection device (4). The output end at the bottom of the ion exchange resin tank (2) is fixedly connected to a concentration device (5).

2. The extraction and concentration device for protein purification ion exchange resin according to claim 1, wherein: The ion exchange resin tank (2) includes a tank body (14), a blanking plate (15), an ion exchange column (16), a discharge cavity (17), and a discharge pipe (18). The tank body (14) is fixedly connected to the top of the support base (1). The inner wall of one end of the tank body (14) is fixedly connected to the blanking plate (15). The output end at the top of the blanking plate (15) is fixedly connected to the ion exchange column (16). A discharge cavity (17) is provided between the blanking plate (15) and the bottom of the inner wall of the tank body (14). The output end at the bottom of the tank body (14) is fixedly connected to the discharge cavity (17).

3. The extraction and concentration device for protein purification ion exchange resin according to claim 2, wherein: A connecting pipe (6) is fixedly connected to the outer wall of one side of the tank body (14), and the connecting pipe (6) penetrates through one side of the inner wall of the tank body (14). The output end on one side of the connecting pipe (6) is located above the blanking plate (15). The output end on the side of the connecting pipe (6) away from the tank body (14) is fixedly connected to a suction pump (7). The output end at the bottom of the suction pump (7) is fixedly connected to a discharge pipe (8).

4. An extraction and concentration device for protein purification ion exchange resin according to claim 3, characterized in that: The elution device (3) includes an eluent injection tank (9), an input pump (10), a second connecting pipe (11), a partition plate (12), and a spray head (13). The eluent injection tank (9) is fixedly connected to the outer wall of the tank body (14) on the side away from the connecting pipe (6). The output end on one side of the eluent injection tank (9) is fixedly connected to the input pump (10). The output end on the side of the input pump (10) away from the eluent injection tank (9) is fixedly connected to the second connecting pipe (11), and the second connecting pipe (11) penetrates through one side of the inner wall of the tank body (14). A partition plate (12) is fixedly connected to the inner wall of the tank body (14) at the end away from the blanking plate (15). The output end at the bottom of the partition plate (12) is fixedly connected to the spray head (13), and the output end on the side of the second connecting pipe (11) away from the input pump (10) is located above the partition plate (12).

5. An extraction and concentration device for protein purification ion exchange resin according to claim 2, characterized in that: The sample injection device (4) includes a blanking pipe (19), a second input pump (20), and a material pipe (21). The output end at the top of the tank body (14) is fixedly connected to the blanking pipe (19), and the blanking pipe (19) penetrates through the top of the inner wall of the tank body (14). The output end at the bottom of the blanking pipe (19) penetrates through the bottom of the partition plate (12). The output end at the top of the blanking pipe (19) is fixedly connected to the second input pump (20). The output end on the side of the second input pump (20) away from the blanking pipe (19) is fixedly connected to the material pipe (21).

6. The extraction and concentration device for protein purification ion exchange resin according to claim 2, wherein: The concentration device (5) includes a bottom plate (22), a support plate (23), a pressure pump (24), a discharge tank (25), an ultrafiltration tank (26), a second ultrafiltration tank (27), a connecting pipe (28), a third input pump (29), a third connecting pipe (30), a shunt pipe (31), a fourth connecting pipe (32), a fifth connecting pipe (33), a confluence pipe (34), and a discharge valve (35). The output end of the discharge pipe (18) away from the tank body (14) is fixedly connected to a connecting pipe (28). The output end of the connecting pipe (28) on the side away from the discharge pipe (18) is fixedly connected to a third input pump (29). The bottom of the third input pump (29) is fixedly connected to a bottom plate (22). On the side of the top of the bottom plate (22) away from the third input pump (29), there is a fixedly connected support plate (23). On the outer wall of one side of the support plate (23), there is a fixedly connected pressure pump (24). On the outer wall of the support plate (23) near the pressure pump (24), there is a fixedly connected discharge tank (25). On the outer wall of the end of the support plate (23) away from the pressure pump (24), there is a fixedly connected ultrafiltration tank (26). On the outer wall of the support plate (23) near the ultrafiltration tank (26), there is a fixedly connected second ultrafiltration tank (27). The output end of the third input pump (29) on the side away from the connecting pipe (28) is fixedly connected to a third connecting pipe (30). The output end of the third connecting pipe (30) on the side away from the third input pump (29) is fixedly connected to the pressure pump (24). The output end of the pressure pump (24) on the side away from the third connecting pipe (30) is fixedly connected to a shunt pipe (31). The output end of the shunt pipe (31) at the end away from the pressure pump (24) is respectively fixedly connected to the output ends at the tops of the ultrafiltration tank (26) and the second ultrafiltration tank (27). The output end at the bottom of the ultrafiltration tank (26) is fixedly connected to a fourth connecting pipe (32). The output end at the bottom of the second ultrafiltration tank (27) is fixedly connected to a fifth connecting pipe (33). And the output ends on one side of the fourth connecting pipe (32) and the fifth connecting pipe (33) are fixedly connected to a confluence pipe (34). The output end of the confluence pipe (34) on the side away from the fourth connecting pipe (32) and the fifth connecting pipe (33) is fixedly connected to the output end at the bottom of the discharge tank (25). The output end of the discharge tank (25) near the confluence pipe (34) is fixedly connected to a discharge valve (35).

7. An extraction and concentration device for protein purification ion exchange resin according to claim 4, characterized in that: There are multiple groups of the spray heads (13) and the ion exchange columns (16).

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