Immune cell sorting device

Abstract

The utility model discloses an immune cell sorting device, which belongs to the technical field of cell sorting and comprises an automatic clamping jaw for grabbing and moving an immune cell test tube, a magnetic bead dropper for dropping a magnetic bead reagent into the immune cell test tube, and a rack which is arranged on the magnetic bead dropper and is used for supporting the magnetic bead dropper, the rotating table is arranged below the magnetic bead dropper and is used for rotationally conveying the immune cell test tube; and a material mixing structure for assisting in mixing cells in the immune cell test tube and a magnetic bead reagent is arranged on the rotating table. After a magnetic bead reagent is dropped into immune cells in a test tube, the motor can be started, and the tray can drive the immune cell test tube to rotate under the action of the motor, so that the cells in the test tube and the magnetic beads are promoted to move, and the magnetic beads and the immune cells are mixed under the action of centrifugal force; the combining efficiency of the magnetic beads and the cells is improved, so that the subsequent sorting effect is optimized.

Description

Technical Field

[0001] The utility model relates to the technical field of cell sorting, and particularly relates to an immune cell sorting device. Background Art

[0002] Immune cell sorting is a technique for separating specific cell subsets from a mixed cell sample according to cell characteristics.

[0003] Immune cell magnetic bead sorting is an efficient and specific cell separation technique widely used in biomedical research. First, after washing and filtering through a filter membrane, cell counting is performed to ensure an appropriate cell density. Then, magnetic beads are combined with specific antibodies and mixed with target cells so that the magnetic beads bind to the surface markers of the target cells. Finally, the cell suspension combined with the magnetic beads is placed in a magnetic field, and the target cells move towards the magnetic field direction to achieve separation.

[0004] Chinese Patent Publication No. CN221344540U discloses an immune cell sorting device, which includes a rotating base and a placement table connected to the rotating base. A plurality of sample tube slots are evenly opened on the placement table. A magnetic bead dropper is arranged at the upper end of the placement table, and the magnetic bead dropper is clamped on a bracket; a clamping device is arranged on the opposite side of the bracket, the placement table is arranged between the clamping device and the bracket, the clamping device includes a frame, a first driving motor and a first air cylinder are arranged on the frame, the first driving motor drives the first air cylinder to rotate on the frame, a first support rod is horizontally connected to the first air cylinder, and automatic clamping claws are arranged at both ends of the first support rod. The beneficial effects are: the clamping device replaces hand-held sample tubes, reducing the risk of contamination and improving the accuracy of the experiment.

[0005] However, in the above patent, only by dropping the magnetic bead reagent into the sample test tube for mixing, the mixing efficiency is reduced, which easily leads to uneven distribution of magnetic beads in the cell mixture, affecting the binding efficiency between the magnetic beads and the target cells, and further affecting the subsequent sorting effect. Summary of the Utility Model

[0006] Aiming at the above technical deficiencies, the purpose of the utility model is to provide an immune cell sorting device to solve some or all of the problems in the above background art.

[0007] To solve the above technical problems, the utility model adopts the following technical solutions:

[0008] An immune cell sorting device, comprising:

[0009] An automatic clamping claw for grasping and moving an immune cell test tube;

[0010] A magnetic bead dropper for dropping a magnetic bead reagent into the immune cell test tube;

[0011] A frame, arranged on the magnetic bead dropper, providing support for the magnetic bead dropper;

[0012] A rotating table, arranged below the magnetic bead dropper, for rotating and conveying the immune cell test tube;

[0013] A mixing structure, arranged on the rotating table, for assisting in mixing the cells and magnetic bead reagent in the immune cell test tube;

[0014] A swinging structure, arranged on the mixing structure, used in cooperation with the mixing structure, for enabling the immune cell test tube to swing to improve the mixing effect;

[0015] Among them, the swinging structure includes an adjusting component for adjusting the angle of the immune cell test tube, a clamping component for preventing the immune cell test tube from falling off during the mixing process, and a linkage component for controlling the opening and closing of the clamping component;

[0016] Multiple groups of the mixing structure and the swinging structure are arranged.

[0017] Preferably, the mixing structure includes:

[0018] A connecting seat, arranged on the rotating table;

[0019] A linkage gear ring, rotatably installed on the connecting seat;

[0020] A rotating rod, rotatably installed on the inner wall of the linkage gear ring;

[0021] A connecting shaft, arranged on the inner wall of the linkage gear ring, and the connecting shaft is rotatably installed on the rotating rod, providing a fulcrum for the rotation of the rotating rod;

[0022] A tray, arranged at one end of the rotating rod, for placing the immune cell test tube;

[0023] A driving component, arranged on the rotating table, for driving the immune cell test tube to rotate.

[0024] Preferably, the driving component includes:

[0025] A linkage gear, rotatably installed on the rotating table;

[0026] A motor, arranged below the rotating table, and the output end of the motor penetrates through the rotating table and is installed on the linkage gear;

[0027] Among them, the linkage gear and the linkage gear ring are meshed with each other.

[0028] Preferably, the adjusting component includes:

[0029] An extrusion inclined block, corresponding to the rotating rod, for extruding the rotating rod and causing it to flip;

[0030] The extrusion ring is arranged on the extrusion wedge block;

[0031] The guide rod is arranged on the linkage gear ring. The guide rod is slidably installed on the extrusion ring and is used to guide the moving direction of the extrusion ring;

[0032] The arc-shaped abutting rod is arranged above the extrusion ring;

[0033] The abutting inclined rod is arranged at one end of the arc-shaped abutting rod;

[0034] The mounting bracket is arranged on the abutting inclined rod and provides support for the abutting inclined rod;

[0035] The limiting component is arranged on the extrusion ring and is used to limit the position of the extrusion ring.

[0036] Preferably, the limiting component includes:

[0037] The spring is slidably sleeved on the guide rod;

[0038] The stop block is arranged at one end of the guide rod;

[0039] Wherein, the two ends of the spring are respectively installed on the sides of the extrusion ring and the linkage gear ring close to each other and are used to reset the movement of the extrusion ring. The stop block is located above the extrusion ring and is used to prevent the extrusion ring from falling off.

[0040] Preferably, the clamping component includes:

[0041] Two clamping blocks are both slidably installed on the tray;

[0042] Two connecting rods are respectively rotatably installed on one side of the two clamping blocks;

[0043] The U-shaped connecting rod is rotatably installed on the two connecting rods;

[0044] Wherein, the U-shaped connecting rod is slidably sleeved on the tray and is used to drive the two connecting rods to move.

[0045] Preferably, the linkage component includes:

[0046] The T-shaped limiting rod is arranged below the linkage gear ring;

[0047] The linkage sliding hole is opened on the T-shaped limiting rod;

[0048] The linkage shaft is movably installed in the linkage sliding hole;

[0049] Wherein, the linkage shaft is arranged on one side of the U-shaped connecting rod.

[0050] The beneficial effects of the utility model are as follows:

[0051] In the present utility model, after dropping magnetic bead reagent into the immune cells in the test tube, the motor can be started. Under the action of the motor, the tray can drive the immune cell test tube to rotate, promoting the movement of the cells and magnetic beads in the test tube, enabling the magnetic beads and immune cells to be mixed under the action of centrifugal force, improving the binding efficiency between the magnetic beads and cells, and thus enhancing the subsequent sorting effect.

[0052] In the present utility model, during the process of the rotating table driving the linkage gear ring and the extrusion ring to move, the extrusion ring can be squeezed by the arc-shaped push rod to drive the tray and the test tube to be in an inclined state. At the same time, the two clamping blocks will clamp the test tube to prevent it from falling off. At this time, under the drive of the motor, the test tube can perform circular motion in the inclined state, enabling the immune cell test tube to achieve a swinging effect, further enabling the magnetic beads to fully mix and contact with the immune cells, and improving the purity of subsequent cell sorting. Description of the Drawings

[0053] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0054] Figure 1 Structural schematic diagram of an immune cell sorting device provided by an embodiment of the present utility model;

[0055] Figure 2 Structural schematic diagram of the linkage gear ring of an immune cell sorting device provided by an embodiment of the present utility model;

[0056] Figure 3 Structural schematic diagram of the rotating rod of an immune cell sorting device provided by an embodiment of the present utility model;

[0057] Figure 4 Structural schematic diagram of the inclined state of the tray of an immune cell sorting device provided by an embodiment of the present utility model;

[0058] Figure 5 Cross-sectional structural schematic diagram of the connection seat of an immune cell sorting device provided by an embodiment of the present utility model.

[0059] Description of the reference numerals:

[0060] 1. Automatic clamping jaw; 2. Magnetic bead dropper; 3. Frame; 4. Rotating table; 5. Connecting seat; 501. Linkage gear ring; 502. Rotating rod; 503. Connecting shaft; 504. Tray; 505. Linkage gear; 506. Motor; 6. Extrusion inclined block; 601. Extrusion ring; 602. Guide rod; 603. Arc-shaped abutting rod; 604. Abutting pressure inclined rod; 605. Mounting bracket; 606. Spring; 607. Stopper; 7. Clamping block; 701. Connecting rod; 702. U-shaped connecting rod; 703. T-shaped limiting rod; 704. Linkage sliding hole; 705. Linkage shaft. Detailed implementation manner

[0061] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0062] Embodiment 1:

[0063] As Figures 1 to 5 shown, the present invention provides an immune cell sorting device, including an automatic clamping jaw 1 for grasping and moving an immune cell test tube, a magnetic bead dropper 2 for dripping a magnetic bead reagent into the immune cell test tube, a frame 3 arranged on the magnetic bead dropper 2 to provide support for the magnetic bead dropper 2, and a rotating table 4 arranged below the magnetic bead dropper 2 for rotating and conveying the immune cell test tube.

[0064] In order to improve the mixing effect of immune cells and magnetic beads in the immune cell test tube and make the magnetic beads fully contact with the immune cells, a mixing structure for assisting in mixing the cells and the magnetic bead reagent in the immune cell test tube is arranged on the rotating table 4, and multiple groups of the mixing structures are arranged.

[0065] Among them, the mixing structure includes a connecting seat 5 arranged on the rotating table 4, a linkage gear ring 501 rotatably installed on the connecting seat 5, a rotating rod 502 rotatably installed on the inner wall of the linkage gear ring 501, a connecting shaft 503 arranged on the inner wall of the linkage gear ring 501 to provide a rotation fulcrum for the rotating rod 502, the connecting shaft 503 is rotatably installed on the rotating rod 502, a tray 504 arranged at one end of the rotating rod 502 for placing the immune cell test tube, a driving component arranged on the rotating table 4 for driving the immune cell test tube to rotate. Starting the motor 506 can drive the two rotating rods 502 to perform circular motion, so that the rotating rods 502 drive the tray 504 to rotate, and then drive the test tube containing cells and the magnetic bead reagent to rotate.

[0066] Specifically, the driving assembly includes a linkage gear 505 rotatably mounted on the rotating table 4, a motor 506 arranged below the rotating table 4, the output end of the motor 506 passes through the rotating table 4 and is mounted on the linkage gear 505, the linkage gear 505 and the linkage gear ring 501 are engaged with each other, and the motor 506 is turned on. The output end of the motor 506 can drive the linkage gear 505 to rotate, so that the rotating linkage gear 505 drives the linkage gear ring 501 to rotate on the connecting seat 5 by engaging with the linkage gear ring 501.

[0067] Embodiment 2:

[0068] On the basis of Example 1, in order to mix the immune cells and magnetic beads more fully, a swinging structure is arranged on the mixing structure to enable the immune cell test tube to swing to improve the mixing effect. There are multiple groups of swinging structures, and the swinging structure includes an adjustment component for adjusting the angle of the immune cell test tube, a clamping component to prevent the immune cell test tube from falling off during the mixing process, and a linkage component to control the opening and closing of the clamping component.

[0069] The adjusting assembly includes an extrusion bevel block 6 for extruding the rotating rod 502 and flipping it relative to the rotating rod 502, an extrusion ring 601 arranged on the extrusion bevel block 6, a guide rod 602 arranged on the linkage gear ring 501 for guiding the moving direction of the extrusion ring 601, the guide rod 602 is slidably mounted on the extrusion ring 601, an arc-shaped push rod 603 arranged above the extrusion ring 601, a pressing bevel rod 604 arranged at one end of the arc-shaped push rod 603, and a pressing bevel rod arranged on the pressing bevel rod 604. 604 provides support for the mounting frame 605, and a limit assembly is arranged on the extrusion ring 601 for limiting the position of the extrusion ring 601. The extrusion ring 601 will be squeezed by the pressing inclined rod 604 and the arc-shaped pressing rod 603 during the continuous movement and slide on the guide rod 602. While the extrusion ring 601 slides, it will drive the extrusion inclined block 6 to squeeze the rotating rod 502. The squeezed rotating rod 502 will drive the tray 504 to move in a circular motion, thereby causing the immune cell test tubes in the tray 504 to be in a tilted state.

[0070] Specifically, the limiting assembly includes a spring 606 that is slidably sleeved on the guide rod 602 for resetting the movement of the extrusion ring 601, and a stopper 607 arranged at one end of the guide rod 602 for preventing the extrusion ring 601 from falling off. The stopper 607 is located above the extrusion ring 601. The two ends of the spring 606 are respectively installed on the side where the extrusion ring 601 and the linkage gear ring 501 are close to each other. The sliding extrusion ring 601 will compress the spring 606. When the continuously moving linkage gear ring 501 breaks away from contact with the arc-shaped support rod 603, the compressed spring 606 will push the extrusion ring 601 to move and reset.

[0071] Among them, the clamping assembly includes two clamping blocks 7 slidably mounted on the tray 504, two connecting rods 701 respectively rotatably mounted on one side of the two clamping blocks 7, a U-shaped connecting rod 702 rotatably mounted on the two connecting rods 701. The U-shaped connecting rod 702 is slidably sleeved on the tray 504 and is used to drive the two connecting rods 701 to move. During the movement of the U-shaped connecting rod 702, it will pull the two connecting rods 701 to move, so that the two connecting rods 701 rotate on the two clamping blocks 7 respectively and pull the two clamping blocks 7 to approach each other. The two clamping blocks 7 that approach each other can finally clamp the outer wall of the immune cell test tube.

[0072] Among them, the linkage assembly includes a T-shaped limiting rod 703 arranged below the linkage gear ring 501, a linkage sliding hole 704 opened on the T-shaped limiting rod 703, a linkage shaft 705 movably mounted in the linkage sliding hole 704. The linkage shaft 705 is arranged on one side of the U-shaped connecting rod 702. While the tray 504 makes a circular motion, it can drive the U-shaped connecting rod 702 to make a circular motion, so that the U-shaped connecting rod 702 drives the linkage shaft 705 to move in the linkage sliding hole 704 on the T-shaped limiting rod 703.

[0073] Working principle:

[0074] The immune cell test tube is grabbed by the automatic gripper 1 and placed in the tray 504, and then the rotating table 4 is used to drive the tray 504 to move in a circular motion and transport it to the bottom of the magnetic bead dropper 2. The magnetic bead reagent is dripped into the immune cell test tube through the magnetic bead dropper 2. At this time, the motor 506 can be turned on, and the output end of the motor 506 can drive the linkage gear 505 to rotate, so that the rotating linkage gear 505 drives the linkage gear ring 501 to rotate on the connecting seat 5 through engagement with the linkage gear ring 501. The continuously rotating linkage gear ring 501 can drive the two rotating rods 502 to move in a circular motion, so that the rotating rod 502 drives the tray 504 to rotate. When the tray 504 rotates, it can drive the cells and the magnetic bead reagent to rotate. The test tube of the bead reagent rotates to improve the binding efficiency of the magnetic beads and cells. During the circular motion of the rotating table 4, the squeezing ring 601 can contact the pressing inclined rod 604. The squeezing ring 601 will be squeezed by the pressing inclined rod 604 and the arc-shaped pressing rod 603 during the continuous movement and slide on the guide rod 602. At the same time, the sliding squeezing ring 601 will compress the spring 606. When the squeezing ring 601 slides, it will drive the squeezing inclined block 6 to squeeze the rotating rod 502. The squeezed rotating rod 502 will rotate on the connecting shaft 503. The rotating rotating rod 502 will drive the tray 504 to move in a circular motion, thereby making the immune cell test tubes in the tray 504 in a tilted state, and the tray 504 When the U-shaped connecting rod 702 is in circular motion, the U-shaped connecting rod 702 drives the linkage shaft 705 to move in the linkage sliding hole 704 on the T-shaped limit rod 703. During the process, the U-shaped connecting rod 702 is affected by the linkage sliding hole 704 and the linkage shaft 705 to pull the two connecting rods 701 and move them, so that the two connecting rods 701 rotate on the two clamping blocks 7 respectively and pull the two clamping blocks 7 closer to each other. The two clamping blocks 7 approaching each other can eventually clamp on the outer wall of the immune cell test tube to prevent it from loosening. At this time, the continuous rotation of the linkage gear ring 501 can drive the tilted immune cell test tube to rotate, and at the same time, the clamping block 7, the U-shaped connecting rod 702 and the T-shaped limit rod 7 03 can follow it to make circular motion, so that the immune cell test tube can achieve a swinging effect, further improving the mixing effect. During the process, the rotating linkage gear ring 501 can drive the squeezing ring 601 to rotate through the guide rod 602, and the rotating squeezing ring 601 will maintain contact with the arc-shaped push rod 603, thereby avoiding the test tube from resetting. When the continuously moving linkage gear ring 501 is out of contact with the arc-shaped push rod 603, the spring 606 in the compressed state will push the squeezing ring 601 to move and reset, so that the rotating rod 502 is no longer squeezed by the squeezing ramp 6. At this time, the tray 504 will flip and reset due to the influence of its own gravity, so that the test tube will return to a vertical state so that the automatic clamp 1 can re-grasp it.

[0075] Obviously, those skilled in the art can make various modifications and variations to the present utility model without departing from the spirit and scope of the present utility model. Thus, if these modifications and variations of the present utility model fall within the scope of the claims of the present utility model and their equivalent technologies, the present utility model also intends to include these modifications and variations therein.

Claims

1. An immune cell sorting device, characterized in that, Comprising: An automatic gripper (1) for grasping and moving the immune cell test tube; A magnetic bead dropper (2) for dropping magnetic bead reagents into the immune cell test tube; A frame (3) arranged on the magnetic bead dropper (2) to provide support for the magnetic bead dropper (2); A turntable (4) arranged below the magnetic bead dropper (2) for rotating and conveying the immune cell test tube; A mixing structure arranged on the turntable (4) for assisting in mixing the cells and magnetic bead reagents in the immune cell test tube; A swinging structure arranged on the mixing structure and used in cooperation with the mixing structure for enabling the immune cell test tube to swing to improve the mixing effect; Wherein, the swinging structure includes an adjusting component for adjusting the angle of the immune cell test tube, a clamping component for preventing the immune cell test tube from falling off during the mixing process, and a linkage component for controlling the opening and closing of the clamping component; Multiple groups of the mixing structure and the swinging structure are arranged.

2. The immune cell sorting device according to claim 1, wherein The mixing structure includes: A connecting seat (5) arranged on the turntable (4); A linkage gear ring (501) rotatably installed on the connecting seat (5); A rotating rod (502) rotatably installed on the inner wall of the linkage gear ring (501); A connecting shaft (503) arranged on the inner wall of the linkage gear ring (501), and the connecting shaft (503) is rotatably installed on the rotating rod (502) to provide a fulcrum for the rotation of the rotating rod (502); A tray (504) arranged at one end of the rotating rod (502) for placing the immune cell test tube; A driving component arranged on the turntable (4) for driving the immune cell test tube to rotate.

3. The immune cell sorting device according to claim 2, wherein The driving component includes: A linkage gear (505) rotatably installed on the turntable (4); A motor (506) arranged below the turntable (4), and the output end of the motor (506) penetrates through the turntable (4) and is installed on the linkage gear (505); Wherein, the linkage gear (505) meshes with the linkage gear ring (501).

4. An immune cell sorting device according to claim 1, characterized in that, The adjusting component includes: A pressing inclined block (6) corresponding to the rotating rod (502) for pressing the rotating rod (502) to make it flip; A pressing ring (601) arranged on the pressing inclined block (6); A guiding rod (602) arranged on the linkage gear ring (501), and the guiding rod (602) is slidably installed on the pressing ring (601) for guiding the moving direction of the pressing ring (601); An arc-shaped abutting rod (603) arranged above the pressing ring (601); A pressing inclined rod (604) arranged at one end of the arc-shaped abutting rod (603); A mounting bracket (605) arranged on the pressing inclined rod (604) to provide support for the pressing inclined rod (604); A limiting component arranged on the pressing ring (601) for limiting the position of the pressing ring (601).

5. The immunocyte sorting device according to claim 4, wherein The limiting component includes: A spring (606) slidably sleeved on the guiding rod (602); A stopper (607) arranged at one end of the guiding rod (602); Among them, both ends of the spring (606) are respectively installed on the side where the extrusion ring (601) and the linkage gear ring (501) are close to each other, and are used to reset the movement of the extrusion ring (601). The stopper (607) is located above the extrusion ring (601) and is used to prevent the extrusion ring (601) from falling off.

6. The immunocyte sorting device according to claim 1, wherein, The clamping assembly includes: Two clamping blocks (7), both of which are slidably installed on the tray (504); Two connecting rods (701), which are respectively rotatably installed on one side of the two clamping blocks (7); A U-shaped connecting rod (702), which is rotatably installed on the two connecting rods (701); Among them, the U-shaped connecting rod (702) is slidably sleeved on the tray (504) and is used to drive the two connecting rods (701) to move.

7. The immunocyte sorting device according to claim 1, wherein, The linkage assembly includes: A T-shaped limiting rod (703), which is arranged below the linkage gear ring (501); A linkage sliding hole (704), which is opened on the T-shaped limiting rod (703); A linkage shaft (705), which is movably installed in the linkage sliding hole (704); Among them, the linkage shaft (705) is arranged on one side of the U-shaped connecting rod (702).

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