Device for rapidly extracting nematode sample in pure soil

Abstract

The utility model provides a pure soil nematode sample rapid extraction device, which comprises a funnel, a soil sieve, a culture dish and a waste liquid cylinder, the bottom of the funnel is communicated with a vertical bottom pipe, the bottom outlet of the vertical bottom pipe is located right above the culture dish, the soil sieve is placed in the funnel, first filter paper is arranged in the soil sieve, and second filter paper is arranged in the funnel. The side wall of the vertical bottom pipe is obliquely and upwards connected with a branch pipe which is connected with a waste liquid cylinder through a hose. The device is scientific and reasonable in structural design, simple to operate, short in extraction time and capable of effectively saving time and labor cost.

Description

Technical Field

[0001] The utility model belongs to the technical field of soil environment, and particularly relates to a device for rapidly extracting pure soil nematode samples. Background Art

[0002] In the soil ecosystem, soil nematodes are rich in species, large in number, and widely distributed, and their traces can be found in all soils on the earth. The soil environment provides conditions suitable for the survival or life of nematode communities, affecting and regulating their community diversity and distribution. At the same time, nematodes affect the surrounding soil environment by participating in soil processes such as nutrient cycling. Therefore, they are interdependent and restrictive. Exploring the interaction relationship between soil nematode communities and their surrounding environment (including biotic and abiotic) can reveal the ecological processes and functions of complex underground ecosystems.

[0003] With the application of sequencing technology in the field of nematode research, the requirement for the purity of nematode samples is getting higher and higher. The existing method for obtaining pure soil nematode samples is to add an extraction process of the Baermann funnel method on the basis of the traditional shallow dish method. This method involves two long-term static placements, with complicated operations, long extraction time, and high time and labor costs. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is to provide a device for rapidly extracting pure soil nematode samples in view of the above-mentioned deficiencies of the prior art. The structure is scientifically and reasonably designed, easy to operate, with a short extraction time, and can effectively save time and labor costs.

[0005] To solve the above technical problem, the technical solution adopted by the utility model is: a device for rapidly extracting pure soil nematode samples, characterized in that it includes a funnel, a soil sieve, a culture dish, and a waste liquid cylinder. The bottom of the funnel is connected to a vertical bottom tube, and the bottom outlet of the vertical bottom tube is located directly above the culture dish. A soil sieve is placed inside the funnel, a first filter paper is arranged inside the soil sieve, a second filter paper is arranged inside the funnel, a branch pipe is obliquely and upwardly connected to the side wall of the vertical bottom tube, and the branch pipe is connected to the waste liquid cylinder through a hose.

[0006] Preferably, a first scale line is arranged on the side wall of the soil sieve, a second scale line is arranged on the side wall of the funnel, a third scale line is arranged at the position corresponding to the connection of the branch pipe on the vertical bottom tube. The first scale line is arranged at the position corresponding to the bottom surface of the first filter paper, and the second scale line is arranged at the position corresponding to the bottom plane of the soil sieve.

[0007] Preferably, the included angle between one end of the branch pipe and the side wall of the vertical bottom tube is 45°, the other end of the branch pipe is vertically connected to a transfer pipe, and the transfer pipe is connected to the waste liquid cylinder through a hose.

[0008] Preferably, a valve is provided on the vertical bottom tube, and a pinch clamp is provided on the flexible hose.

[0009] Preferably, the top diameter of the funnel is 25 cm, the diameter of the soil sieve is 20 cm, and the mesh diameter of the soil sieve is 2 cm.

[0010] The utility model has the following advantages compared with the prior art:

[0011] The structure of the utility model is designed scientifically and reasonably, simple to operate, can effectively save the extraction time, has good extraction effect, low manufacturing cost, and can be popularized and used.

[0012] The following further describes the utility model in detail with reference to the drawings and embodiments. Description of the Drawings

[0013] Figure 1 is the front view structural schematic diagram of the utility model.

[0014] Figure 2 is the front view sectional structural schematic diagram of the utility model.

[0015] Figure 3 is the front view structural schematic diagram of the soil sieve in the utility model.

[0016] Description of the Reference Numerals:

[0017] 1 - soil sieve; 2 - funnel; 3 - first filter paper;

[0018] 4 - second filter paper; 5 - second scale line; 6 - vertical bottom tube;

[0019] 7 - valve; 8 - third scale line; 9 - branch pipe;

[0020] 10 - transfer pipe; 11 - flexible hose; 12 - waste liquid cylinder;

[0021] 13 - pinch clamp; 14 - culture dish; 15 - first scale line. Detailed Embodiment

[0022] As Figures 1 to 3 shown, the utility model includes a funnel 2, a soil sieve 1, a culture dish 14, and a waste liquid cylinder 12. The bottom of the funnel 2 is connected to a vertical bottom tube 6. The bottom outlet of the vertical bottom tube 6 is located directly above the culture dish 14. The soil sieve 1 is placed inside the funnel 2. A first filter paper 3 is arranged inside the soil sieve 1. A second filter paper 4 is arranged inside the funnel 2. The side wall of the vertical bottom tube 6 is obliquely upwardly connected to a branch pipe 9. The branch pipe 9 is connected to the waste liquid cylinder 12 through a flexible hose 11.

[0023] In this embodiment, a first scale line 15 is provided on the side wall of the soil sieve 1, a second scale line 5 is provided on the side wall of the funnel 2, a third scale line 8 is provided at the position on the vertical bottom tube 6 corresponding to the connection of the branch pipe 9, the first scale line 15 is provided at the position corresponding to the bottom surface of the first filter paper 3, and the second scale line 5 is provided at the position corresponding to the bottom plane of the soil sieve 1.

[0024] In this embodiment, the included angle between one end of the branch pipe 9 and the side wall of the vertical bottom tube 6 is 45°, the other end of the branch pipe 9 is vertically connected to the transfer pipe 10, and the transfer pipe 10 is connected to the waste liquid cylinder 12 through a hose 11.

[0025] In this embodiment, a valve 7 is provided on the vertical bottom tube 6, and a clamp 13 is provided on the hose 11.

[0026] In this embodiment, the top diameter of the funnel 2 is 25 cm, the diameter of the soil sieve 1 is 20 cm, and the mesh diameter of the soil sieve 1 is 2 cm.

[0027] The operation method of the present utility model is as follows:

[0028] S1. Close the valve 7 and the clamp 13, inject distilled water into the funnel 2 to the second scale line 5, and open the clamp 13 to fill the branch pipe 9 with distilled water; [[ID=??]]

[0029] S2. Place the soil sieve 1 on the funnel 2, slowly pour the washed soil and nematode suspension into the soil sieve 1, and control the water level in the soil sieve 1 to just cover the first scale line 15 by adding distilled water or adjusting the clamp 13;

[0030] S3. Let it stand for 24 h. During the nematode enrichment process, nematodes are hydrophilic and will swim downward due to gravity. The impurities in the suspension are filtered through the first filter paper 3 and the second filter paper 4 to obtain a clean nematode sample. Finally, the nematodes will gather in the vertical bottom tube 6 and be blocked by the closed valve 7 to form a nematode enrichment area. The branch pipe inclined upward at 45° can ensure that nematodes do not enter the branch pipe 9, resulting in a reduction in the number of nematodes and affecting the accuracy of the test results;

[0031] S4. After standing, remove the soil sieve 1, open the clamp 13 to allow the excess liquid to flow into the waste liquid cylinder 12. Under the siphon action, the water level will drop to the third scale line 8, and then open the valve 7 to collect the clean nematode sample into the culture dish 14 for the next test.

[0032] The above is only a preferred embodiment of the present utility model, and it does not impose any limitations on the present utility model. Any simple modifications, changes, and equivalent variations made to the above embodiments according to the technical essence of the utility model still fall within the protection scope of the technical solution of the present utility model.

Claims

1. An apparatus for rapidly extracting a pure soil nematode sample, characterized in that It includes a funnel (2), a soil sieve (1), a culture dish (14) and a waste liquid cylinder (12). The bottom of the funnel (2) is connected to a vertical bottom pipe (6), and the bottom outlet of the vertical bottom pipe (6) is located directly above the culture dish (14). The soil sieve (1) is placed inside the funnel (2), a first filter paper (3) is arranged inside the soil sieve (1), a second filter paper (4) is arranged inside the funnel (2), the side wall of the vertical bottom pipe (6) is obliquely upward connected to a branch pipe (9), and the branch pipe (9) is connected to the waste liquid cylinder (12) through a hose (11).

2. The device for rapidly extracting a pure soil nematode sample according to claim 1, wherein, First scale lines (15) are arranged on the side wall of the soil sieve (1), second scale lines (5) are arranged on the side wall of the funnel (2), third scale lines (8) are arranged at the position on the vertical bottom pipe (6) corresponding to the connection of the branch pipe (9), the first scale lines (15) are arranged at the position corresponding to the bottom surface of the first filter paper (3), and the second scale lines (5) are arranged at the position corresponding to the bottom plane of the soil sieve (1).

3. The device for rapidly extracting a pure soil nematode sample according to claim 1, wherein, The included angle between one end of the branch pipe (9) and the side wall of the vertical bottom pipe (6) is 45°, the other end of the branch pipe (9) is perpendicularly connected to a transfer pipe (10), and the transfer pipe (10) is connected to the waste liquid cylinder (12) through a hose (11).

4. The device for rapid extraction of a pure soil nematode sample according to claim 1, characterized in that, A valve (7) is arranged on the vertical bottom pipe (6), and a clamp (13) is arranged on the hose (11).

5. The device for rapid extraction of pure soil nematode samples according to claim 1, characterized in that, The top diameter of the funnel (2) is 25 cm, the diameter of the soil sieve (1) is 20 cm, and the mesh diameter of the soil sieve (1) is 2 cm.

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