Cold trap, access device and biological sample storage system

Abstract

The utility model provides a cold trap, an access device and a biological sample storage system.The cold trap comprises a cold trap box with a containing cavity, at least one end of the cold trap box is provided with an opening used for sample feeding or sample discharging, and the opening is communicated with the containing cavity; the cold trap box is provided with an upper box body wall located on the upper portion and a lower box body wall connected to the lower portion of the upper box body wall, the upper box body wall is provided with a cavity used for containing refrigerants, the lower box body wall comprises a perforated plate, the perforated plate is provided with a flow channel communicated with the cavity, and the end, away from the cavity, of the flow channel extends downwards. The flow channel comprises a plurality of areas communicated with pore channels in the perforated plate in the extending direction of the flow channel, so that a refrigerant in the cavity can flow into the perforated plate. According to the cold trap box, the refrigerant in the cavity can flow into the flow channel and then flow into the perforated plate on the wall of the lower box body, so that the refrigerant can quickly enter the perforated plate, the temperature of the accommodating cavity in the cold trap box can be quickly reduced to the temperature required for storing the sample box, and the time required for sampling is shortened.

Description

[0001] This application claims the priority of a patent application with the application number 2024215000588, the application date of June 27, 2024, and the patent title of "Cold Trap, Access Device, and Biological Sample Storage System". Technical Field

[0002] This application relates to the technical field of biological sample storage, and more particularly, to a cold trap, an access device, and a biological sample storage system. Background Art

[0003] In the prior art, biological samples are mostly stored in sample cans. When sampling, the sample box needs to be taken out of the sample can first, and then the sample is picked out from the sample box. In order to keep the sample box in a low-temperature environment after it is taken out of the sample can, a separate refrigeration structure needs to be set up in the prior art to refrigerate the taken-out sample box. The existing refrigeration structure is difficult to achieve rapid refrigeration, thus increasing the sampling time. Summary of the Utility Model

[0004] The purpose of this application is to provide a cold trap, an access device, and a biological sample storage system to quickly provide a low-temperature environment, thereby shortening the sampling time.

[0005] The embodiments of this application are implemented as follows:

[0006] In a first aspect, an embodiment of this application provides a cold trap for a biological sample storage system, including a cold trap box having a receiving cavity. At least one end of the cold trap box has an opening for sample injection or extraction, and the opening communicates with the receiving cavity. The cold trap box is provided with an upper box body wall located at the upper part and a lower box body wall connected to the lower part of the upper box body wall. The upper box body wall is provided with a cavity for accommodating a refrigerant. The lower box body wall includes a porous plate, and a flow channel communicating with the cavity is provided at the porous plate. The end of the flow channel away from the cavity extends downward. The flow channel includes a plurality of regions communicating with the pore channels in the porous plate in its extending direction, so that the refrigerant in the cavity can flow into the porous plate.

[0007] In the above technical solution, the cavity of the upper box body wall of the cold trap box can be used to accommodate the refrigerant. The end of the flow channel away from the cavity extends downward, and the flow channel has a plurality of regions communicating with the pore channels in the porous plate. The refrigerant in the cavity can flow into the flow channel under the action of gravity, and then flow into the porous plate of the lower box body wall of the cold trap box through multiple positions of the flow channel, so that the refrigerant can quickly enter the porous plate, and further enable the temperature of the receiving cavity in the cold trap box to quickly drop to the temperature required for storing the sample box, shortening the sampling time.

[0008] In combination with the first aspect, in some embodiments, it further includes a pipeline, the lumen of the pipeline is the flow channel, along the extending direction of the pipeline, the pipeline and the porous plate have a plurality of contacting regions, and the regions where the pipeline contacts the porous plate have through holes.

[0009] In the above technical solution, the flow channel is arranged in the pipeline, and the through holes arranged in the pipeline can enable the refrigerant in the flow channel to flow out of the flow channel. And the through holes are arranged in the regions where the pipeline contacts the porous plate, so that the refrigerant can flow into the pore channels of the porous plate after flowing out. The structure and installation of the above technical solution are simpler and easier to maintain.

[0010] In combination with the first aspect, in some embodiments, the lower box body wall includes a plurality of porous plates arranged at intervals in the circumferential direction, the extending direction of each porous plate is the same as the extending direction of the pipeline, the pipeline is clamped between two adjacent porous plates, the lower end of the pipeline is a closed end, and through holes are arranged in the regions where the pipeline contacts each porous plate.

[0011] In the above technical solution, the pipeline is clamped between two adjacent porous plates, and through holes are arranged in the regions where the pipeline contacts each porous plate, so that one pipeline can supply refrigerant to two porous plates, improving the cooling speed of the porous plates. In addition, since the lower end of the pipeline is closed, when the refrigerant reaches the lower end of the pipeline, the refrigerant accumulates in the pipeline, facilitating the refrigerant to flow into the pore channels of the porous plate and improving the utilization rate of the refrigerant.

[0012] In combination with the first aspect, in some alternative embodiments, the lower box body wall is all the porous plates, there are multiple flow channels arranged at intervals in the circumferential direction in the porous plates, and at least a part of the circumferential direction of the flow channels contacts the porous plates.

[0013] In the above technical solution, the flow channels are arranged in the porous plates. After the refrigerant in the cavity flows into the flow channels, part of the refrigerant flows along the flow channels, and the other part of the refrigerant enters the pore channels of the porous plates, realizing the uniform distribution of the refrigerant in the porous plates and achieving the effect of uniform and rapid refrigeration.

[0014] In combination with the first aspect, in some alternative embodiments, a heat insulation plate is arranged outside the cold trap box, and a part of the circumferential direction of the flow channel contacts the porous plate, and the other part contacts the heat insulation plate.

[0015] In the above technical solution, the outside of the flow channel is a heat insulation plate to prevent the loss of cold from the outside, and the inside of the flow channel is a porous plate, which can enable the refrigerant to penetrate into the porous plate after entering the flow channel, so as to achieve rapid refrigeration.

[0016] In combination with the first aspect, in some alternative embodiments, the porous plate is a porous plate capable of adsorbing liquid nitrogen.

[0017] In the above technical solution, liquid nitrogen can be used for refrigeration, which can rapidly reduce the temperature in the accommodation chamber.

[0018] Combined with the first aspect, in some alternative embodiments, the cross-sectional area of the flow channel is in the range of 0.7 mm 2 to 314.0 mm 2 The cross-sectional area of the pores of the porous plate is in the range of 0.19×10 -6 mm 2 to 0.2 mm 2 and the cross-sectional area of the flow channel / the cross-sectional area of the pores of the porous plate ≥ 10.

[0019] Combined with the first aspect, in some alternative embodiments, the lower box body wall is further provided with a first sampling port for taking out the sample in the accommodation chamber.

[0020] Combined with the first aspect, in some alternative embodiments, the upper box body wall is provided with a liquid injection hole for injecting refrigerant, and the liquid injection hole communicates with the cavity.

[0021] In a second aspect, the present application provides an access device, a lifting mechanism, a top plate, and a cold trap provided in the first aspect. The top plate covers the upper end of the cold trap box, the opening is located at the lower end of the cold trap box, the lifting mechanism is provided on the top plate and at least partially located in the accommodation chamber, and the lifting mechanism is configured to take samples into or out of the cold trap box.

[0022] In the above technical solution, the top plate is provided at the upper end of the cold trap box, the opening for sampling is located at the lower end of the cold trap box, and the lifting mechanism is installed on the top plate. The sample is pulled by the lifting mechanism so that the sample is taken into the accommodation chamber of the cold trap box from the opening, and the sample during sampling can still be in the low-temperature environment of the cold trap box. After sampling, the sample can be sent out of the accommodation chamber of the cold trap box. At the same time, setting the lifting mechanism on the top plate and at least partially located in the accommodation chamber can facilitate the fixing of the lifting mechanism to save space, and can also facilitate directly taking the sample into the cold trap box to avoid affecting the temperature of the sample.

[0023] Combined with the second aspect, in some embodiments, the lifting mechanism includes a lifting part and an extraction part. The lifting part is provided on the top plate, the extraction part is partially located in the accommodation chamber, and the lifting part is drivingly connected to the extraction part for taking samples into or out of the cold trap box through the extraction part.

[0024] In the above technical solution, the extraction part is used to extract the sample, and the lifting part is used to drive the extraction part to move up and down for sampling.

[0025] In combination with the second aspect, in some embodiments, the extraction part includes a vertically extending conductive sheet, a magnetic head with an electromagnet, and a conductive contact rod electrically connected to the electromagnet. The conductive sheet is disposed in the cold trap box. The conductive contact rod is slidably disposed on the conductive sheet and is in electrical contact with the conductive sheet. The lifting part is drivingly connected to the magnetic head to vertically slide the conductive contact rod on the conductive sheet.

[0026] In the above technical solution, the extraction part includes a magnetic head with an electromagnet, and the basket for placing the sample can be adsorbed by magnetic attraction, thereby pulling the basket to move. Since the electromagnet is electrically connected to a conductive contact rod, and the conductive contact rod is in electrical contact with the conductive sheet, when the lifting part drives the magnetic head to move, the conductive contact rod slides on the conductive sheet, so that the conductive contact rod remains in electrical contact with the conductive sheet. Therefore, by supplying power to the conductive sheet, continuous power supply to the electromagnet can be achieved. By using the power supply method in the above technical solution, wires are not required, avoiding the problem of wire entanglement during the movement of the magnetic head.

[0027] In combination with the second aspect, in some embodiments, vertically extending guide rails are connected to two opposite sides of the cold trap box. Both sides of the extraction part are slidably disposed on the guide rails. By providing guide rails on both sides of the extraction part, the stability of the extraction part during movement can be effectively improved.

[0028] In combination with the second aspect, in some embodiments, the guide rail has two vertically extending tracks, and at least part of the two tracks overlap in the height direction. A first slider is slidably connected to one track, and the first slider is fixedly connected to the cold trap box. A second slider is slidably connected to the other track, and the second slider is fixedly connected to the extraction part.

[0029] In the above technical solution, each guide rail includes two tracks, and at least part of the two tracks overlap in the height direction; the extraction part is slidably connected to one track through the second slider, and the other track is fixedly connected to the cold trap box through the first slider, so that when the extraction part moves up and down under the action of the lifting part, the guide rail can also move up and down. Since the guide rail can also move, with the movement stroke of the extraction part remaining unchanged, the structure of the above technical solution can effectively reduce the height of the guide rail, thereby reducing the height of the access device.

[0030] In combination with the second aspect, in some embodiments, the lifting part includes a belt and a driving motor installed on the top plate. The extraction part is fixedly connected to the belt, and the driving motor is drivingly connected to the belt to move the extraction part fixed on the belt up and down.

[0031] In combination with the second aspect, in some embodiments, a first protective cover is further provided on a side of the top plate away from the cold trap box, and the top plate is provided with a through hole for the belt to pass through; the drive motor is installed in a space enclosed by the top plate and the first protective cover.

[0032] In the above technical solution, by installing the drive motor on the side of the top plate away from the cold trap box, the drive motor is located outside the cold trap box's housing, preventing the drive motor from operating in a low-temperature environment and effectively reducing the possibility of drive motor failure. By installing the drive motor in the space enclosed by the top plate and the first shield, it can not only protect the drive motor, but also reduce the loss of cold energy within the housing chamber.

[0033] In combination with the second aspect, in some embodiments, a first roller is provided on one side of the cold trap box, and a second roller is provided on the other opposite side; the first roller and the second roller are used to contact the basket pulled by the extraction part from two opposite sides.

[0034] In the above technical solution, the first roller and the second roller contact two opposite sides of the basket, which can reduce the shaking of the basket when it moves under the traction of the extraction part, and make the movement of the basket more stable.

[0035] In combination with the second aspect, in some embodiments, the first roller is connected to a telescopic member, and the telescopic member is used to apply a force to the first roller toward the side where the second roller is located.

[0036] According to the above technical solution, a telescopic part is set. During each sampling process, when the extraction part pulls the basket to move, the basket remains in contact with the second roller under the action of the first roller, so that the second roller can play a role in positioning the basket. During each sampling process, the movement reference of the basket is the second roller, which can reduce the influence of processing accuracy, assembly accuracy, etc. on the movement process of the basket.

[0037] In combination with the second aspect, in some embodiments, the cold trap box is provided with a roller frame, the roller frame is provided with a slide groove, the slide groove extends from the side where the first roller is located to the side where the second roller is located; the first roller is slidably arranged in the slide groove; the telescopic member is arranged between the roller frame and the first roller.

[0038] In a third aspect, an embodiment of the present application provides a biological sample storage system, including a frame, a storage tank, and the access device provided in the second aspect. The storage tank is provided with a second sampling port, and a basket for placing samples is arranged inside the storage tank; the cold trap is configured to be movably arranged on the frame so that the opening is aligned with the second sampling port; the lifting mechanism is configured to enter the storage tank from the second sampling port and pull the basket into the cold trap box.

[0039] For the biological sample storage system provided by the above technical solution, a second sampling port is arranged in the storage tank, and sampling is performed from above the second sampling port by using the access device provided in the second aspect. Since the access device includes the cold trap provided in the embodiment of the present application, during the sampling process, the temperature of the accommodation cavity in the cold trap box can be quickly reduced to a preset temperature, thereby shortening the time required for sampling.

[0040] In combination with the third aspect, in some embodiments, two opposite sides of the basket are metal plates; the metal plates are provided with through slots; a first roller is arranged on one inner side of the cold trap box, and a second roller is arranged on the other opposite inner side; when the lifting mechanism controls the lifting of the basket, the first roller and the second roller slide in the through slots respectively.

[0041] In the above technical solution, a first roller and a second roller are arranged on two opposite sides of the cold trap box, and through slots are arranged on both sides of the basket on which the sample box is placed. During the process of pulling the basket into the accommodation cavity, the first roller and the second roller slide in the through slots respectively, which can play a role in guiding the basket and reducing the shaking of the basket.

[0042] In combination with the third aspect, in some embodiments, the biological sample storage system further includes a first sampling mechanism. The lower box body wall is further provided with a first sampling port. The first sampling mechanism includes a first sampling part and a shovel plate. The first sampling part is drivingly connected to the shovel plate, and the first sampling part is configured to transfer the shovel plate to the first sampling port so as to take out the reagent kit on the basket in the accommodation cavity through the shovel plate.

[0043] In the above technical solution, the lifting part is installed on the top plate, and the opening for sampling is located at the lower end of the cold trap box. During the sampling process, the extraction part pulls the basket into the accommodation cavity from the lower end of the cold trap box. The cold trap box is provided with a first sampling port for taking out the sample from the accommodation cavity. When the sample to be taken in the basket moves to the position where the first sampling port is located, the sample to be taken can be taken out through the first sampling mechanism. Since the first sampling port is arranged on the lower box body wall and the basket enters the accommodation cavity from the lower end of the cold trap box, the time for pulling the sample to be taken to the position where the first sampling port is located can be reduced, thereby shortening the sampling time.

[0044] In combination with the third aspect, in some embodiments, the biological sample storage system also includes a second sampling mechanism, which includes a second sampling part, a sample box sampling clamp and a sample tube sampling clamp. The second sampling part is connected to the sample box sampling clamp to move the sample box, or the second sampling part is connected to the sample tube sampling clamp to move the sample tube in the sample box.

[0045] In combination with the third aspect, in some embodiments, the second sampling portion is provided with an electromagnet, and the second sampling portion is connected to the sample box sampling clamp or the sample tube sampling clamp by magnetic attraction.

[0046] In combination with the third aspect, in some embodiments, the rack has a liquid nitrogen adding station, and the liquid nitrogen adding station is provided with a liquid adding tube; the upper box body wall is provided with a liquid injection hole for injecting liquid nitrogen; and further includes a moving mechanism, which drives the cold trap box to be connected so that the cold trap box is transferred to the liquid nitrogen adding station, and the liquid adding tube can be selectively inserted into the liquid injection hole to inject liquid nitrogen into the cavity. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0048] Figure 1 This is a partial structural diagram of the biological sample storage system;

[0049] Figure 2 is a schematic diagram of the first sampling mechanism taking a sample from the containing cavity at a first angle;

[0050] Figure 3 is a schematic diagram of the first sampling mechanism taking a sample from the accommodating cavity at a second angle;

[0051] Figure 4 for Figure 2 A schematic diagram showing the cold trap box with the outer shell and thermal insulation panel removed from one side of the cold trap;

[0052] Figure 5 is a schematic cross-sectional view of an access device;

[0053] Figure 6 To use the first plane longitudinal section Figure 2 Schematic diagram of the resulting cross section;

[0054] Figure 7 To use the second plane longitudinal sectionFigure 2 The obtained cross-sectional schematic diagram, where the second plane is perpendicular to Figure 6 the first plane in

[0055] Figure 8 is Figure 7 the enlarged view at position A in

[0056] Figure 9 the schematic diagram of the pipeline provided by this application being spiral;

[0057] Figure 10 the schematic diagram of the first roller and the second roller respectively contacting the basket from both sides;

[0058] Figure 11 the schematic diagram when the extraction part is at the highest position in the vertical direction;

[0059] Figure 12 is when the second slider moves downward relative to the second track to the limit position.

[0060] Icons: 1000 - Biological sample storage system; 1100 - Rack; 1200 - Storage tank; 1210 - Second sampling port; 1220 - Sampling cover; 1300 - Access device; 1310 - Cold trap; 1311 - Cold trap box; 13111 - Accommodation cavity; 13112 - Opening; 13113 - Upper box body wall; 131131 - Cavity; 131132 - Refrigerant box; 1311321 - Flange; 131133 - Liquid injection hole; 131141 - Perforated plate; 131142 - First sampling port; 13116 - Pipeline; 1320 - Heat insulation board; 1330 - Outer shell; 1340 - Liquid injection joint; 1351 - Lifting part; 13511 - Belt; 13512 - Driving motor; 13513 - Counterweight part; 1352 - Extraction part; 13521 - Conductive sheet; 13522 - Magnetic suction head; 13523 - Conductive contact rod; 1360 - Top plate; 1370 - Guide rail; 1371 - First track; 1372 - Second track; 1373 - First slider; 1374 - Second slider; 1381 - First shield; 1382 - Second shield; 1391 - First roller; 1392 - Second roller; 1394 - Roller frame; 13941 - Chute; 1400 - Basket; 1410 - Guide groove; 1500 - First sampling mechanism; 1510 - Shovel plate; 1600 - Cover opening mechanism; 2000 - Sample box. Detailed implementation manners

[0061] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this application. Apparently, the described embodiments are some, but not all, of the embodiments of this application. Usually, the components of the embodiments of this application described and illustrated in the accompanying drawings herein can be arranged and designed in various different configurations.

[0062] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of this application claimed, but merely represents selected embodiments of this application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of this application without creative efforts shall fall within the scope of protection of this application.

[0063] It should be noted that like reference numerals and letters denote like items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0064] In the description of this application, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings or the orientation or positional relationship in which the product of this application is usually placed during use. It is only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. In addition, the terms "first", "second", "third", etc. are only used for descriptive distinction and should not be construed as indicating or implying relative importance.

[0065] In addition, the terms "horizontal", "vertical", "overhanging", etc. do not mean that the components must be absolutely horizontal or overhanging, but may be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but may be slightly inclined.

[0066] In the description of this application, it should also be noted that unless otherwise clearly specified and limited, the terms "set", "installed", "connected", "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be directly connected, or indirectly connected through an intermediate medium, and it may be the internal communication of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0067] In some existing biological sample storage systems, biological samples are contained in sample tubes. A certain batch of sample tubes are then placed in a sample box, and a certain batch of sample boxes are further placed in a carrying basket. The carrying basket is arranged in a storage tank that provides a low-temperature environment to achieve low-temperature storage of biological samples. During the sampling process, the carrying basket is first taken out of the storage tank, then the corresponding sample box is selected from the carrying basket, and finally the sample tube to be sampled is taken from the sample box.

[0068] The present application provides a biological sample storage system 1000, as Figure 1 and Figure 2 shown, including an access device 1300 and a storage tank 1200. Among them, the access mechanism 1300 includes a cold trap 1310 and a lifting mechanism. The cold trap 1310 can provide a low-temperature environment. Further, as Figure 2 and Figure 3 shown, the cold trap 1310 has a first sampling port 131142, and the storage tank 1200 has a second sampling port 1210. The lifting mechanism takes out the carrying basket 1400 in the storage tank 1200 through the second sampling port 1210 into the cold trap 1310, and then takes out the sample box 2000 in the carrying basket 1400 from the first sampling port 131142. Finally, a sampling mechanism is used to take out the required sample tube from the sample box 2000.

[0069] It should be noted that in the biological sample storage system 1000 provided by the present application, in addition to storing a certain batch of sample boxes 2000 in the carrying basket 1400, the sample box 2000 can also be arranged on other carrying structures for storage, as long as the sample box 2000 can be taken out of the storage tank 1200; it can also be that only one sample box 2000 is arranged in the carrying basket 1400 or other carrying structures; the present application does not limit the carrying structure for placing the sample box 2000. In the following of the present application, it is taken that the sample tubes are placed in the sample box 2000, the sample box 2000 is placed in the carrying basket 1400, and the carrying basket 1400 is taken out first and then the sample box 2000 is taken out from the carrying basket 1400 during the sampling process as an example to illustrate the biological sample storage system 1000 provided by the present application.

[0070] In one embodiment, as Figure 3 and Figure 4 shown, the cold trap 1310 includes a cold trap box 1311 having an accommodation cavity 13111. At least one end of the cold trap box 1311 has an opening 13112 for sample injection or extraction, and the opening 13112 communicates with the accommodation cavity 13111 (see Figure 7), so that a sample (i.e., a sample tube containing the sample, or a sample cassette 2000 carrying the sample tube, or a basket 1400, etc.) can enter or leave the accommodation cavity 13111 through the opening 13112. Among them, only one end of the cold trap cassette 1311 may have an opening 13112 for sample injection or extraction; in some other embodiments, both ends of the cold trap cassette 1311 may have an opening 13112 for sample injection or extraction. Further, the opening 13112 for sample injection or extraction means that the opening 13112 can be used for both sample injection and extraction, including the sample entering the accommodation cavity 13111 through the opening 13112 and leaving the accommodation cavity 13111 through the opening 13112 at the other end of the cold trap cassette 1311; it also includes the sample entering the accommodation cavity 13111 through the opening 13112 and then still leaving the accommodation cavity 13111 through the opening 13112, such as Figure 7 As shown in the embodiment, the sample enters or leaves the accommodation cavity 13111 through the opening 13112 at the lower end of the cold trap cassette 1311.

[0071] In some embodiments, such as Figure 4 , Figure 5 , Figure 7 and Figure 8 As shown, the cold trap cassette 1311 has an upper box body wall 13113 located at the upper part and a lower box body wall connected to the lower part of the upper box body wall 13113. The upper box body wall 13113 is provided with a cavity 131131 for accommodating a refrigerant. The lower box wall body includes a porous plate 131141, and a flow channel communicating with the cavity 131131 is provided at the porous plate 131141, so that the refrigerant in the cavity 131131 can enter the porous plate 131141. Among them, the upper box body wall 13113 is the wall body of the cold trap cassette 1311 at the relatively upper part in the use state, specifically including the wall body above the midpoint position in the height direction of the cold trap cassette 1311; correspondingly, the lower box wall body is the wall body below the midpoint position in the height direction of the cold trap cassette 1311. The upper box body wall 13113 is provided with a cavity 131131 for accommodating a refrigerant, which means that the cavity 131131 is arranged inside the upper box wall body; the refrigerant is a substance with a relatively low temperature, such as liquid nitrogen, etc. Further, in some embodiments, the cavity 131131 is arranged around the accommodation cavity 13111 in the circumferential direction, and the porous plate 131141 is also arranged around the accommodation cavity 13111, so that each side of the accommodation cavity 13111 is cooled by the refrigerant, making the temperature distribution in the accommodation cavity 13111 more uniform. Among them, the circumferential direction is the circumferential direction of the accommodation cavity 13111, that is, the extending direction of the edge of the opening 13112 communicated with the accommodation cavity 13111. Further, the upper box body wall 13113 can be a structural member with good thermal conductivity, such as a metal structural member, so that the upper box body wall 13113 can also perform heat exchange with the inside of the accommodation cavity 13111 to achieve a refrigeration effect on the accommodation cavity 13111.

[0072] It is not difficult to understand that the cavity 131131 is located above the porous plate 131141. The porous plate 131141 has a three-dimensional through-hole structure with fine pores inside, and has a certain liquid absorption capillary phenomenon, which can make the refrigerant be more evenly distributed inside the porous plate 131141. The refrigerant in the cavity 131131 can enter the flow channel under the action of gravity and then enter the porous plate 131141. Furthermore, the porous plate 131141 has a uniformly distributed lower temperature under the action of the refrigerant.

[0073] Furthermore, one end of the flow channel far from the cavity 131131 extends downward. The flow channel has a plurality of regions communicating with the pores in the porous plate 131141 in its extending direction, so that the refrigerant in the cavity 131131 can flow into the porous plate 131141. Among them, the flow channel can communicate with the pores of the porous plate 131141 only in two regions, or can communicate with the pores of the porous plate 131141 in three or even more regions. Furthermore, the regions communicating with the pores of the porous plate 131141 are evenly distributed in the extending direction of the flow channel. Among them, the size of each region in the extending direction of the flow channel is not greater than 20 mm.

[0074] Since the extending direction of the flow channel has a plurality of regions communicating with the pores in the porous plate 131141, the refrigerant can enter the porous plate 131141 from multiple positions in the extending direction of the flow channel. Furthermore, the whole porous plate 131141 can have a lower temperature faster and the temperature distribution is more uniform. Furthermore, the temperature of the accommodation cavity 13111 in the cold trap box 1311 can be quickly reduced to the temperature required for accommodating the sample box 2000. Specifically, when the biological sample storage system 1000 is used for the first time, the temperature of the accommodation cavity 13111 in the cold trap box 1311 can be quickly reduced, and before each sampling, the temperature in the cold trap box 1311 can be quickly adjusted to the required low-temperature environment, thereby shortening the time required for sampling.

[0075] Furthermore, in some embodiments, the cross-sectional area of the flow channel is in the range of 0.7 mm 2 ~314 mm 2 Specifically, it can be 0.7 mm 2 、10 mm 2 、50 mm 2 、200 mm 2 、314 mm 2etc., so that the refrigerant can flow rapidly along the flow channels and then reach more positions of the porous plate 131141 quickly; for example, the cross-section of the flow channels can be a square with a side length of 1 mm, or a circle with a diameter of 1 mm, or a square with a side length of 10 mm, or a circle with a radius of 10 mm. The cross-sectional area of the pores in the porous plate 131141 is in the range of 0.19×10 -6 mm 2 ~0.2 mm 2 range, and the cross-sectional area of the flow channels / the cross-sectional area of the pores in the porous plate 131141 ≥ 10, that is, the cross-sectional area of the flow channels is ten times or more than ten times that of the pores. Among them, the cross-sectional area of the flow channels refers to the area of the cross-section perpendicular to the extension direction of the flow channels; the cross-sectional area of the pores in the porous plate 131141 refers to the area of the cross-section perpendicular to the extension direction of the pores. It should be noted that: the pores in the porous plate 131141 are three-dimensional network structures, and the cross-sectional area of the pores in the porous plate 131141 is in the range of 0.19×10 -6 mm 2 ~0.2 mm 2 range, which does not mean that the cross-sectional area at any pore of the porous plate 131141 is in the range of 0.19×10 -6 mm 2 ~0.2 mm 2 range. As long as the cross-sectional area at some pores is in the range of 0.19×10 -6 mm 2 ~0.2 mm 2 range, it is sufficient.

[0076] In some embodiments, the porous plate 131141 can be a vulcanized plate with the cross-sectional area of the pores in the above range; in some other embodiments, the porous plate 131141 can also be a structural member composed of materials such as felt that are easy to adsorb liquids, so that the refrigerant in the flow channels can be evenly distributed in the porous plate 131141. In the existing refrigeration methods, using liquid nitrogen for refrigeration can reach a low temperature of -196°C. If the refrigerant in the cavity 131131 is liquid nitrogen, the accommodation cavity 13111 can be maintained at a relatively low temperature, such as -20°C, -80°C, etc. In the embodiment where the refrigerant is liquid nitrogen, the porous plate 131141 is a porous plate that can adsorb liquid nitrogen; such as Figure 4As shown, a liquid injection hole 131133 is further provided on the upper box body wall 13113. The liquid injection hole 131133 communicates with the cavity 131131 to add liquid nitrogen into the cavity 131131. Further, a temperature sensor for detecting the temperature in the accommodation cavity 13111 can be installed in the cold trap box 1311 or other positions, so as to add liquid nitrogen into the cavity 131131 when the temperature in the accommodation cavity 13111 is relatively high, thereby reducing the temperature in the accommodation cavity 13111. Further, a liquid injection joint 1340 can be connected to the liquid injection hole 131133, and the liquid injection joint 1340 is docked with a liquid adding pipe for transporting a refrigerant, so as to facilitate adding a refrigerant such as liquid nitrogen into the cavity 131131.

[0077] Further, in some embodiments, as Figure 4 shown, the cold trap 1310 further includes a pipe 13116. The pipe 13116 contacts the porous plate 131141 and has a plurality of contact areas in the extending direction of the pipe 13116. The lumen of the pipe 13116 is a flow channel communicating with the cavity 131131. Through holes are provided on the pipe 13116 so that the refrigerant in the flow channel can flow out from the flow channel. Further, the through holes are provided in the area where the pipe 13116 contacts the porous plate 131141, so that the refrigerant can enter the porous plate 131141 better after flowing out. Further, a plurality of through holes can be provided simultaneously in the area where the pipe 13116 contacts the porous plate 131141. The plurality of through holes can be arranged at intervals in the extending direction of the pipe 13116. Of course, only one through hole can be provided in each area. In this embodiment, the area where the flow channel contacts the porous plate 131141 is the area surrounded by the through holes provided on the pipe 13116. During the process of the refrigerant flowing in the pipe 13116, when the refrigerant passes through the through holes of the pipe 13116, part of the refrigerant flows out from the through holes, and the other part of the refrigerant continues to flow downward.

[0078] In some embodiments, the pipe 13116 can be provided inside the porous plate 131141, including partially provided inside the porous plate 131141 and entirely provided inside the porous plate 131141. Among them, the pipe 13116 being partially provided inside the porous plate 131141 means that when the cold trap 1310 is cut by a plane perpendicular to the extending direction of the pipe 13116, the cross-section of the porous plate 131141 has an inward depression to accommodate or avoid the edge of the pipe 13116, and the cross-section of the pipe 13116 partially protrudes from the edge of the cross-section of the porous plate 131141; the pipe 13116 being entirely provided inside the porous plate means that the cross-section of the pipe 13116 does not protrude from the edge of the cross-section of the porous plate 131141. For example, Figure 4In the illustrated embodiment, the lower box body wall includes a plurality of porous plates 131141 arranged circumferentially, and the extending direction of each porous plate 131141 is the same as that of the pipe 13116, and the pipe 13116 is clamped between two porous plates 131141. In other embodiments, grooves may also be provided on the surface of the porous plate 131141, and the pipe 13116 is embedded in the grooves. Further, the lower end of the pipe 13116 is a closed end, that is, after the refrigerant flows to the lower end of the pipe 13116, it accumulates in the pipe 13116 and then overflows from the pore channels of the through holes to the pore channels of the porous plate 131141. By closing the lower end of the pipe 13116, more refrigerant can enter the pore channels of the porous plate 131141 to play a refrigeration role. In other embodiments, the lower end of the pipe 13116 may be an open end. The cross-sectional shape of the pipe 13116 in the present application may be circular, square, or other shapes.

[0079] In some other embodiments, the pipe 13116 may also be entirely disposed outside the porous plate 131141. Specifically, it includes the pipe 13116 being disposed on the side of the porous plate 131141 facing the accommodation cavity 13111, and also includes the pipe 13116 being disposed on the side of the porous plate 131141 away from the accommodation cavity 13111; in the case of having multiple pipes 13116, it may also be that some pipes 13116 are located on the side of the porous plate 131141 facing the accommodation cavity 13111 and another part of the pipes 13116 are located on the side of the porous plate 131141 facing away from the accommodation cavity 13111.

[0080] In some embodiments, the end of the pipe 13116 away from the cavity 131131 may not protrude from the porous plate 131141. In this embodiment, the lower end of the pipe 13116 may not be a closed end. In some embodiments, the end of the pipe 13116 away from the cavity 131131 may also protrude from the porous plate 131141. In this embodiment, the lower end of the pipe 13116 is preferably a closed end, and the through holes of the pipe 13116 are all provided in the area that does not protrude from the porous plate 131141.

[0081] In some embodiments, the pipe 13116 is a straight pipe, that is, the pipe 13116 extends linearly away from the cavity 131131. In this embodiment, the structure of the porous plate 131141 is relatively simple. For example, Figure 4 In the illustrated embodiment, the porous plate 131141 is a rectangular plate-like structural member, and the straight pipe is clamped between two porous plates 131141; in some other embodiments, the groove in the porous plate 131141 for setting the pipe 13116 is strip-shaped, which is convenient for processing.

[0082] In some other embodiments, the pipe 13116 may also be a bent pipe orFigure 9 The tubular structural member extending along a spiral curve shown. In this embodiment, the extension path of the pipeline 13116 is relatively long, and it can pass through more positions in the porous plate 131141, facilitating the refrigerant to be fed into the pores of the porous plate 131141 from more positions more quickly.

[0083] In some embodiments of the present application, the flow channel can also be a pore-like structure provided in the porous plate 131141. The inlet end of the flow channel communicates with the cavity 131131 in the upper box body wall 13113. For example, a through hole is provided at the lower end of the cavity 131131. After the porous plate 131141 is connected to the upper box body wall 13113, the through hole is aligned with the inlet end of the flow channel. For example, in some embodiments, the flow channel is a blind hole provided in the porous plate 131141, and the lower box body wall is the porous plate 131141. Multiple flow channels are arranged at intervals in the porous plate 131141. After the porous plate 131141 is connected to the upper box body wall 13113, the flow channels are arranged at intervals along the circumference of the accommodation cavity 13111. In this embodiment, both the circumference and the lower end of the flow channel are in contact with the porous plate 131141, so that the refrigerant in the flow channel can enter the pores.

[0084] In some other embodiments, the flow channel can also be formed after the porous plate 131141 is connected to other structures. For example, in one embodiment, a strip-shaped groove that penetrates to the surface edge at one end is provided on the surface of the porous plate 131141 away from the accommodation cavity 13111. A plate-shaped structural member is connected to the surface of the porous plate 131141 away from the accommodation cavity 13111 in a surface-contact manner. The blind hole formed by the plate-shaped structural member and the strip-shaped groove on the surface of the porous plate 131141 is the flow channel. The plate-shaped structural member can be a heat insulation plate 1320 for heat insulation, that is, a heat insulation plate 1320 is provided outside the cold trap box 1311. A part of the circumference of the flow channel is in contact with the porous plate 131141, and the other part is in contact with the heat insulation plate 1320. The plate-shaped structural member can also be another porous plate 131141, then both the circumference and the lower end of the flow channel are in contact with the porous plate 131141.

[0085] In some embodiments of the present application, the heat insulation plate 1320 provided outside the cold trap box 1311 completely covers the outside of the cold trap box 1311, that is, the heat insulation plate 1320 covers both the upper box body wall 13113 and the porous plate 131141 to reduce the heat exchange between the cold trap box 1311 and the outside.

[0086] Furthermore, a housing 1330 is also provided on the side of the heat insulation plate 1320 away from the cold trap box 1311, and the cold trap box 1311 is fixedly connected to the housing 1330. In some embodiments, such as Figure 8As shown, the upper box body wall 13113 includes a refrigerant box 131132. A cavity 131131 for accommodating refrigerant is located within the refrigerant box 131132. The lower edge of the refrigerant box 131132 has a downward flange 1311321, and the flange 1311321 is located on the side close to the accommodation cavity 13111. Both the heat insulation plate 1320 and the porous plate 131141 are disposed in the gap between the flange 1311321 and the outer shell 1330. That is, the outer shell 1330, the heat insulation plate 1320, and the porous plate 131141 are sequentially arranged from the outside to the inside. The outer shell 1330 presses the heat insulation plate 1320 and the porous plate 131141 by extrusion and thus presses the heat insulation plate 1320 and the porous plate 131141 against the flange 1311321 of the refrigerant box 131132. Further, in some embodiments, the outer shell 1330 is disposed on the outer periphery of the cold trap box 1311 and is an annular structure formed by connecting multiple plate-like structures. Therefore, the outer shell 1330 can be fixedly connected to the cold trap box 1311 by means of clamping. In other embodiments, the outer shell 1330 and the cold trap box 1311 can also be fixedly connected by means of threaded fasteners or bonding, etc.

[0087] In some embodiments, the access device 1300 further includes a lifting mechanism for feeding a sample into or out of the accommodation cavity 13111 of the cold trap box 1311. As Figure 6 shown, a top plate 1360 is further provided at the upper end of the cold trap box 1311. The lifting mechanism is disposed on the top plate 1360. An opening 13112 for sample injection or extraction in the cold trap box 1311 is located at the lower end of the cold trap box 1311. That is, the lifting mechanism draws the sample into the cold trap box 1311 through the opening 13112 at the lower end of the cold trap box 1311. Since the lifting mechanism is used to feed the sample into the cold trap box 1311, at least part of the lifting mechanism is located within the accommodation cavity 13111. In some embodiments, the lifting mechanism can also be entirely located within the accommodation cavity 13111. Further, in Figure 6 the illustrated embodiment, the top plate 1360 covers the upper end of the cold trap box 1311, which can reduce the loss of cold in the accommodation cavity 13111 from the upper end of the cold trap box 1311. Among them, the top plate 1360 can be connected to the outer shell 1330 or can also be connected to the cold trap box 1311.

[0088] Further, the lifting mechanism includes a lifting part 1351 and an extraction part 1352. Among them, as Figure 6As shown, the lifting part 1351 is installed on the top plate 1360. The lifting part 1351 is drivingly connected to the extraction part 1352, and the extraction part 1352 is used to connect to the basket 1400. The lifting part 1351 is used to drive the extraction part 1352 to lift after the extraction part 1352 is connected to the basket 1400, so as to take the sample into or out of the cold trap box 1311. In an embodiment where part of the lifting mechanism is located in the accommodation cavity 13111, it may be that the extraction part 1352 is located in the accommodation cavity 13111 and the lifting part 1351 is located outside the accommodation cavity 13111; in an embodiment where the entire lifting mechanism is located in the accommodation cavity 13111, both the extraction part 1352 and the lifting part 1351 are located inside the accommodation cavity 13111. Among them, since the extraction part 1352 is a movable component, the extraction part 1352 can be considered to be located inside the accommodation cavity 13111 when it moves into the accommodation cavity 13111 under the drive of the lifting part 1351. Further, in some embodiments, the extraction part 1352 is located inside the accommodation cavity 13111 and can also move outside the accommodation cavity 13111 under the action of the lifting part 1351 to take out the basket 1400 from the storage tank 1200 or achieve other purposes. Of course, in some other embodiments, the extraction part 1352 may be installed on the top plate 1360 and the lifting part 1351 may be installed on the cold trap box 1311.

[0089] In some embodiments of the present application, the basket 1400 for placing the sample box 2000 has an iron structure member, and the extraction part 1352 is connected to the basket 1400 by magnetic attraction. Further, as Figures 5 to 7As shown, the extraction part 1352 includes a magnetic head 13522 with an electromagnet. It is not difficult to understand that when the electromagnet is powered, the magnetic head 13522 can adsorb the basket 1400 by magnetic force. After the sampling is completed and the basket 1400 is sent back into the storage tank 1200, the magnetic head 13522 is powered off. In order to improve problems such as winding of the power supply line for the magnetic head 13522, in some embodiments, the magnetic head 13522 is powered by a sliding contact method. Specifically, the extraction part 1352 further includes a vertically extending conductive sheet 13521 and a conductive contact rod 13523 electrically connected to the electromagnet. It is not difficult to understand that both the conductive sheet 13521 and the conductive contact rod 13523 are conductive structural members, such as metals. The conductive sheet 13521 is disposed in the cold trap box 1311, and the conductive contact rod 13523 is slidably disposed on the conductive sheet 13521 and is also in electrical contact with the conductive sheet 13521. During the process of the lifting part 1351 driving the magnetic head 13522 to move, the conductive contact rod 13523 slides vertically on the conductive sheet 13521. That is, during the movement of the magnetic head 13522, the conductive contact rod 13523 always remains in contact with the conductive sheet 13521. It is not difficult to understand that in the technical solution provided by this embodiment, there will be no problem of wire winding during the movement of the magnetic head 13522. The conductive sheet 13521 in the above embodiment is not limited to a sheet structure. The conductive sheet 13521 can be a sheet structure, a plate structure, or a collector rail structure in the prior art, etc.

[0090] In Figure 5 In the structure shown, the conductive sheet 13521 is disposed on one side of the cold trap box 1311 and is fixedly connected to the housing 1330. One side of the cold trap box 1311 has a vertically extending notch for avoiding the conductive sheet 13521. In some other embodiments, the cold trap box 1311 can also be a structure with a rectangular cross-section, and the conductive sheet 13521 is disposed inside the cold trap box 1311.

[0091] In some other embodiments, the extraction part 1352 can also be connected to the basket 1400 by means of negative pressure adsorption, etc.; the extraction part 1352 can also be a structure such as a manipulator, which is connected to the basket 1400 by grasping.

[0092] In order to make the movement of the extraction part 1352 smoother, such as Figure 5As shown, the cold trap box 1311 is connected to a vertically extending guide rail 1370, and the extraction part 1352 is slidably connected to the guide rail 1370. That is, the extending direction of the guide rail 1370 is the same as the moving direction of the extraction part 1352. Among them, the guide rail 1370 can be the outer shell 1330 connected to the outside of the cold trap box 1311, or can be directly connected to the cold trap box 1311. Further, the number of the guide rails 1370 is two. That is, the extraction part 1352 is simultaneously slidably connected to the two guide rails 1370, which can make the movement process of the extraction part 1352 more stable. Further, the two guide rails 1370 are arranged on both sides of the cold trap box 1311. It is not difficult to understand that since the extraction part 1352 moves in the accommodation cavity 13111, the extraction part 1352 is located between the two guide rails 1370, and each of the two opposite sides of the extraction part 1352 is slidably connected to a guide rail 1370, which can not only make the movement of the extraction part 1352 more stable, but also make the force on the extraction part 1352 more balanced.

[0093] Further, in order to reduce the size of the guide rail 1370 in the vertical direction, and then reduce the space occupied by the access device 1300 in the vertical direction. The guide rail 1370 has two vertically extending tracks, and at least part of the two tracks overlap in the height direction, where the height direction is also the vertical direction. As Figure 5 shown, the two tracks are denoted as the first track 1371 and the second track 1372. The first track 1371 is slidably connected to a first slider 1373 fixedly connected to the cold trap box 1311, and the second track 1372 is slidably connected to a second slider 1374 fixedly connected to the extraction part 1352. Among them, the fact that the first slider 1373 is fixedly connected to the cold trap box 1311 means that the cold trap box 1311 and the first slider 1373 are relatively fixed and can move together (the first track 1371 is slidably connected to the first slider 1373, and the first slider 1373 is fixedly connected to the cold trap box 1311), rather than restricting the first slider 1373 to be directly fixed to the cold trap box 1311; the first slider 1373 and the cold trap box 1311 can both be fixedly connected to the outer shell 1330. Similarly, the second slider 1374 and the extraction part 1352 are also relatively fixed and move together with the extraction part 1352 (the second track 1372 is slidably connected to the second slider 1374, and the second slider 1374 is fixedly connected to the extraction part 1352). It is not difficult to understand that the guide rail 1370 is slidably connected to both the cold trap box 1311 and the extraction part 1352.

[0094] Please refer to Figure 11 and Figure 12, when the extraction part 1352 is at the highest position in the vertical direction, the first slider 1373 is located at the lowermost end of the first track 1371, and the second slider 1374 is located at the uppermost end of the second track 1372; during the downward movement of the extraction part 1352, the first track 1371, the second track 1372 and the extraction part 1352 move downward together. The first slider 1373 guides the guide rail 1370, and at the same time, the guide rail 1370 also guides and restricts the movement of the extraction part 1352, enabling the extraction part 1352 to move relatively smoothly; when the first slider 1373 moves relative to the upper limit position of the first track 1371, during the process of the lifting part 1351 driving the extraction part 1352 to continue moving downward, the first slider 1373, the first track 1371, the second track 1372 and the cold trap box 1311 are relatively stationary, and the second slider 1374 and the extraction part 1352 move downward along the second track 1372. When the second slider 1374 moves downward relative to the second track 1372 to the limit position, the extraction part 1352 also moves to the limit position.

[0095] It is not difficult to understand from the above movement process that the guide rail 1370 moves downward under the action of gravity; since the first track 1371 and the second track 1372 partially overlap in the height direction, the guide rail 1370 will also move during the movement of the extraction part 1352. When the required movement distances of the extraction part 1352 are equal, compared with the implementation mode in which the guide rail 1370 only includes one track, the above implementation mode can reduce the size of the guide rail 1370 in the vertical direction, and thus reduce the height of the access device 1300. Optionally, the first track 1371 and the second track 1372 are parallel and completely overlap in the height direction, that is, the upper ends of the first track 1371 and the second track 1372 are at the same height, and the lower ends of the first track 1371 and the second track 1372 are at the same height; in another implementation mode, the first track 1371 and the second track 1372 may also partially overlap in the height direction or the first track 1371 and the second track 1372 overlap by half in the height direction, etc.

[0096] It is not difficult to understand that the connection between the first track 1371 and the second track 1372 is relatively fixed, and the first track 1371 and the second track 1372 move together. Further, the connection method between the first track 1371 and the second track 1372 can be welding connection, threaded fastener fixed connection, etc. In some other implementation modes, the first track 1371 and the second track 1372 can also be arranged on the same guide rail 1370 so that the relative position relationship between the first track 1371 and the second track 1372 will not change. In some other implementation modes, a guide post can also be used to replace the guide rail 1370 to guide the extraction part 1352.

[0097] In some embodiments of the present application, Figure 3 、 Figure 4 and Figure 6 As shown, the lifting part 1351 includes a belt 13511 and a driving motor 13512 installed on the top plate 1360, the extraction part 1352 is fixed to the belt 13511, and the driving motor 13512 drives the connecting belt 13511 to make the extraction part 1352 fixed on the belt 13511 move up and down. Figure 6 Belt 13511 is not connected to form a closed loop structure. One end of belt 13511 is fixedly connected to the extraction part 1352, and the other end is connected to the counterweight part 13513. The middle part of belt 13511 is connected to the drive motor 13512. It is not difficult to understand that the output shaft of the drive motor 13512 is provided with a pulley, and belt 13511 is assembled on the pulley. During the rotation of the drive motor 13512, the pulley provides a force for belt 13511, so that belt 13511 can drive the drive part to move. Because one end of belt 13511 is connected to the counterweight part 13513 and the other end is connected to the extraction part 1352, belt 13511 maintains good contact with the drive motor 13512 under the action of the gravity of the counterweight part 13513 and the extraction part 1352. Furthermore, in order to enable the drive motor 13512 to better drive the belt 13511 to move, the belt 13511 used is a synchronous belt. In some other embodiments, the extraction unit 1352 may be driven by the drive motor 13512 in a chain drive manner; for example, Figure 6 The belt 13511 shown in the figure is replaced with a chain, and accordingly, the pulley connected to the drive motor 13512 is replaced with a sprocket. In some embodiments, the counterweight portion 13513 is arranged outside the accommodating cavity 13111. Further, the counterweight portion 13513 is arranged on the side of the housing 1330 away from the accommodating cavity 13111. In some embodiments, the side of the housing 1330 away from the accommodating cavity 13111 is further connected to a second shield 1382, such as Figure 3 As shown, the cross section of the second shield 1382 perpendicular to the movement direction of the extraction part 1352 is in the shape of a Chinese character "凵", and one end of the belt 13511 connected to the counterweight part 13513 is located in the space enclosed by the second shield 1382 and the shell 1330.

[0098] In other embodiments of the present application, the extraction portion 1352 may also be composed of a screw nut and the like, wherein the extraction portion 1352 is connected to the nut, and the screw is the lifting portion 1351. The extraction portion 1352 may also be other mechanisms in the prior art that can achieve lifting.

[0099] Please refer to Figure 4 and Figure 6, in an embodiment where the lifting part 1351 is located outside the accommodation cavity 13111, the driving motor 13512 in the lifting part 1351 can be installed on the side of the top plate 1360 away from the accommodation cavity 13111. A through hole for the belt 13511 to pass through is provided on the top plate 1360. In order to protect structures such as the driving motor 13512 and reduce the loss of cold in the accommodation cavity 13111, a first protective cover 1381 is also connected to the side of the top plate 1360 away from the accommodation cavity 13111. The driving motor 13512 and the belt 13511 are installed in the space enclosed by the top plate 1360 and the first protective cover 1381. Compared with setting the driving motor 13512 in the accommodation cavity 13111, the embodiment of setting the driving motor 13512 on the top plate 1360 away from the accommodation cavity 13111 can enable the driving motor 13512 to work in an environment with a relatively higher temperature, reducing the situation of the driving motor 13512 failing to move in a low-temperature environment.

[0100] When a certain batch of sample boxes 2000 are placed on the basket 1400, the vertical distribution of the sample boxes 2000 on the basket 1400 will make the basket 1400 have a relatively large size in the vertical direction. When the vertical size of the basket 1400 is relatively large, during the process of the extraction part 1352 pulling the basket 1400 to move, the basket 1400 may have a relatively large shake. Therefore, in some embodiments, a first roller 1391 is provided on one side of the cold trap box 1311, and a second roller 1392 is provided on the other opposite side. The first roller 1391 and the second roller 1392 are used to contact the basket 1400 pulled by the extraction part 1352 from two opposite sides to limit the shake of the basket 1400 during the movement along with the extraction part 1352. It is not difficult to understand that the first roller 1391 and the second roller 1392 can roll. Since the first roller 1391 and the second roller 1392 are provided on the cold trap box 1311, during the process of pulling the basket 1400 into the accommodation cavity 13111, the positions of the first roller 1391 and the second roller 1392 remain unchanged. After the first roller 1391 and the second roller 1392 contact the basket 1400, the first roller 1391 and the second roller 1392 roll along with the lifting of the basket 1400 to reduce the resistance to the movement of the basket 1400.

[0101] In some embodiments, the first roller 1391 and the second roller 1392 may be disposed below the cold trap 1310, that is, at a position below the opening 13112 at the lower end of the cold trap box 1311; in other embodiments, the first roller 1391 and the second roller 1392 may also be disposed inside the accommodation cavity 13111. In the present application, the number of the first roller 1391 and the second roller 1392 may be one, or two or more; when there are multiple first rollers 1391, the first rollers 1391 may be all disposed at the same height, or Figure 7 and Figure 10 disposed at different height positions as shown, for example, Figure 7 shown, some of the first rollers 1391 are disposed inside the accommodation cavity 13111, and the other part of the first rollers 1391 are located below the cold trap 1310. Further, the first roller 1391 and the second roller 1392 are preferably symmetrically disposed along a plane so that the acting forces exerted by the first roller 1391 and the second roller 1392 on the basket 1400 can cancel each other out, avoiding the situation that the basket 1400 tilts; as Figure 7 and Figure 10 shown, the first roller 1391 and the second roller 1392 are both disposed at the same height.

[0102] In some embodiments, the first roller 1391 is connected to a telescopic member, and the telescopic member is used to apply a force to the first roller 1391 toward the second roller 1392, so that after the first roller 1391 contacts the basket 1400, a force is applied to the basket 1400 toward the second roller 1392. In this embodiment, the second roller 1392 can serve as a reference for positioning the basket 1400. That is, by making the basket 1400 contact the second roller 1392 during each sampling process, the movement trajectory of the basket 1400 has a high degree of consistency during each sampling process, reducing the influence of the processing accuracy, assembly accuracy, and the position accuracy of the basket 1400 in the storage tank 1200 on the movement of the basket 1400 during the sampling process. Further, the telescopic member may be an elastic structural member such as a spring. Due to its own elastic structure, it can be telescoped under an external force, and when it is disposed in a compressed state on the side of the first roller 1391 away from the second roller 1392, a force can be applied to the first roller 1391 toward the second roller 1392. The telescopic member may also be a telescopic structural member such as a cylinder or an electric push rod. By applying a force to the first roller 1391 through the cylinder, the first roller 1391 can be pushed in the direction of the second roller 1392, and then a force is applied to the basket 1400 toward the second roller 1392.

[0103] Further, in the embodiment where the telescopic member is a spring, the cold trap box 1311 is provided with a roller frame 1394. The roller frame 1394 can be directly connected to the cold trap box 1311, or connected to the outer shell 1330 outside the cold trap box 1311, or provided on other structures. As Figure 10 shown, the roller frame 1394 is provided with a chute 13941. The chute 13941 extends from the side where the first roller 1391 is located to the side where the second roller 1392 is located. The first roller 1391 is slidably disposed in the chute 13941 so that the first roller 1391 can move toward the side where the second roller 1392 is located; the telescopic member is disposed between the first roller 1391 and the roller frame 1394, that is, one end of the telescopic member contacts the roller frame 1394 and the other end contacts the first roller 1391; it is not difficult to understand that the telescopic member is located on the side of the first roller 1391 away from the second roller 1392 to apply a force toward the side where the second roller 1392 is located to the basket 1400.

[0104] In some embodiments of the present application, as Figure 10 shown, two opposite sides of the basket 1400 are metal plates. The metal plates are provided with guide grooves 1410 that penetrate at least the upper ends. The dimensions of the guide grooves 1410 are set with reference to the dimensions of the first roller 1391 and the second roller 1392. During the process of the extraction part 1352 pulling the basket 1400 into the accommodation cavity 13111, the guide grooves 1410 provided at the corresponding positions of the basket 1400 pass by the first roller 1391 and the second roller 1392 respectively. The first roller 1391 and the second roller 1392 roll relative to each other in the guide grooves 1410, which can play a role in guiding the movement of the basket 1400 to a certain extent and reduce the shaking of the basket 1400. Further, the metal plates on both sides of the basket 1400 have a certain thickness, and the guide grooves 1410 are provided on the metal plates by a subtractive processing method so that the basket 1400 has better rigidity. The metal plates on both sides of the basket 1400 are preferably aluminum plates or aluminum alloy plates to reduce the overall weight of the basket 1400. In some embodiments, the guide grooves 1410 can penetrate both the upper and lower ends.

[0105] In some embodiments of the application, the extraction part 1352 pulls the basket 1400 into the accommodation cavity 13111 from the lower end opening 13112 of the cold trap box 1311. The first sampling port 131142 is provided on the lower box body wall of the cold trap box 1311 so that the sample box 2000 with the sample tube to be taken placed in the basket 1400 passes by the first sampling port 131142 earlier. Correspondingly, through holes or notches aligned with the first sampling port 131142 are provided at the corresponding positions of the heat insulation plate 1320 and the outer shell 1330 so that the sample can be taken out of the accommodation cavity 13111 from the first sampling port 131142; further, the lower side of the first sampling port 131142 penetrates the cold trap box 1311. AsFigure 4 In Figure 5 the illustrated embodiment, the cold trap box 1311 has a quadrangular prism structure, the first sampling port 131142 and the conductive sheet 13521 are disposed on two opposite sides of the cold trap box 1311, and the two guide rails 1370 are disposed on the other two opposite sides of the cold trap box 1311. Of course, in some other embodiments, the first sampling port 131142 may also be disposed at other positions, and those skilled in the art can make adaptive adjustments according to the sampling method.

[0106] In some embodiments of the present application, as Figure 1 illustrated, the biological sample storage system 1000 further includes a sampling cover 1220 covering the second sampling port 1210, an opening mechanism 1600 for opening the sampling cover 1220, a first sampling mechanism 1500 disposed on the frame 1100, and a second sampling mechanism disposed on the frame 1100.

[0107] The opening mechanism 1600 and the access device 1300 are both movably disposed on the frame 1100, and the access device 1300 can be moved to a position where the opening 13112 of the cold trap box 1311 is aligned with the second sampling port 1210. As Figure 4 、 Figure 6 In Figure 7 the illustrated embodiment, the first sampling mechanism 1500 includes a first sampling part and a shovel plate 1510. During the sampling process, the first sampling part is used to drive the shovel plate 1510 to move horizontally so as to enter the accommodation cavity 13111 from the first sampling port 131142 and be located below a sample box 2000 in the basket 1400 that is drawn into the accommodation cavity 13111; then the first sampling part drives the shovel plate 1510 to move in the vertical direction to lift the sample box 2000 from the basket 1400, so that the sample box 2000 is carried on the shovel plate 1510, and then the first sampling part drives the shovel plate 1510 to move horizontally out of the accommodation cavity 13111 with the sample box 2000, so as to take out the sample box 2000 from the basket 1400 to the outside of the accommodation cavity 13111. Among them, the first sampling part can be composed of a mechanism capable of realizing linear motion in the prior art. For example, in some embodiments, the first sampling part includes a third slider slidable relative to the frame 1100 and a fourth slider slidably connected to the third slider, and the shovel plate 1510 is connected to the fourth slider; the third slider drives the fourth slider and the shovel plate 1510 to move together so that the shovel plate 1510 moves into the accommodation cavity 13111, and the fourth slider drives the shovel plate 1510 to move to lift the sample box 2000 from the basket 1400.

[0108] In some embodiments, the second sampling mechanism includes a second sampling part, a sample box sampling clamp, and a sample tube sampling clamp. Among them, the sample box sampling clamp can hold the sample box 2000 to transfer the sample box 2000; the sample tube sampling clamp can hold a single sample tube in the sample box 2000 to pick out the required sample tube. Both the sample box sampling clamp and the sample tube sampling clamp can be detachably connected to the second sampling part. That is, the second sampling part can be connected to the sample box sampling clamp to transfer the sample box 2000 taken out by the shovel plate 1510; the second sampling part can also be connected to the sample tube sampling clamp.

[0109] Furthermore, the second sampling part is provided with an electromagnet, and both the sample box sampling clamp and the sample tube sampling clamp are provided with iron blocks. The second sampling part realizes the connection with the sample box sampling clamp and the sample tube sampling clamp by magnetic attraction. The magnetic attraction method can facilitate the replacement connection of the second sampling part between the sample box sampling clamp and the sample tube sampling clamp.

[0110] In some embodiments, the frame 1100 also has a liquid nitrogen adding station and a moving mechanism. The liquid nitrogen adding station is provided with a liquid adding pipe; the moving mechanism is drivingly connected to the outer shell 1330 of the cold trap box 1311 to move the cold trap box 1311 relative to the frame 1100. The specific movements include: driving the cold trap box 1311 to transfer to the liquid nitrogen adding station to add liquid nitrogen; and driving the cold trap box 1311 to move to a position where the lower opening 13112 is aligned with the second sampling port 1210 for the sampling process. Further, when the cold trap box 1311 is at the liquid nitrogen adding station, the liquid adding pipe can be selectively inserted into the liquid injection hole 131133 to inject liquid nitrogen into the cavity 131131; in some embodiments, it can also be that the liquid adding pipe is docked with the liquid injection joint 1340 to inject liquid nitrogen into the cavity 131131.

[0111] The process of sampling by the biological sample storage system 1000 provided by the embodiments of the present application includes the steps:

[0112] S1: Use the lid opening mechanism 1600 to open the sampling lid 1220 covering the second sampling port 1210;

[0113] S2: Move the access device 1300 above the second sampling port 1210 to align the opening 13112 at the lower end of the cold trap box 1311 with the second sampling port 1210;

[0114] S3: The drive motor 13512 installed on the top plate 1360 in the lifting part 1351 drives the magnetic head 13522 of the extraction part 1352 to sequentially pass through the opening 13112 at the lower end of the cold trap box 1311 and the second sampling port 1210 of the storage tank 1200 from the accommodation cavity 13111 and then enter the storage tank 1200; then, the electromagnet of the magnetic head 13522 is powered by the conductive sheet 13521 and the conductive contact rod 13523, so that the basket 1400 is adsorbed on the magnetic head 13522.

[0115] S4: The drive motor 13512 of the lifting part 1351 drives the magnetic head 13522 of the extraction part 1352 to move upward to pull the basket 1400 into the accommodation cavity 13111.

[0116] S5: When the sample box 2000 where the sample to be taken is located passes through the first sampling port 131142, the drive motor 13512 is stopped from rotating, and the basket 1400 stops moving.

[0117] S6: The first sampling part drives the shovel plate 1510 to move and takes out the sample box 2000 staying at the position of the first sampling port 131142 from the basket 1400.

[0118] S7: The second sampling part is used to connect the sample box sampling clip, and then the sample box 2000 taken out by the shovel plate 1510 is clamped through the sample box sampling clip; or the second sampling part is used to connect the sample tube sampling clip, and then the sample tube in the sample box 2000 taken out by the shovel plate 1510 is clamped through the sample tube sampling clip.

[0119] S8: The second sampling part transfers the clamped sample box 2000 to a preset position or transfers the clamped sample tube to a preset position.

[0120] The above are only the preferred embodiments of the present application and are not used to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cold trap, characterized in that, It includes a cold trap box with a receiving cavity, at least one end of the cold trap box having an opening for sample injection or extraction, and the opening communicating with the receiving cavity; the cold trap box is provided with an upper box body wall located at the upper part and a lower box body wall connected to the lower part of the upper box body wall, the upper box body wall being provided with a cavity for accommodating a refrigerant, the lower box body wall including a porous plate, and a flow channel communicating with the cavity being provided at the porous plate, and the end of the flow channel away from the cavity extending downward; the flow channel includes a plurality of regions communicating with the pore channels in the porous plate in its extending direction, so that the refrigerant in the cavity can flow into the porous plate.

2. The cold trap according to claim 1, wherein It further includes a pipeline, the lumen of the pipeline being the flow channel, and along the extending direction of the pipeline, the pipeline has a plurality of contacting regions with the porous plate, and the regions where the pipeline contacts the porous plate have through holes.

3. The cold trap according to claim 2, wherein The lower box body wall includes a plurality of porous plates arranged at circumferential intervals, the extending direction of each porous plate being the same as the extending direction of the pipeline, the pipeline being clamped between two adjacent porous plates, the lower end of the pipeline being a closed end, and through holes being provided in the regions where the pipeline contacts each porous plate.

4. The cold trap according to claim 1, characterized in that, The lower box body wall is all the porous plate, and there are a plurality of flow channels arranged at circumferential intervals in the porous plate, and at least a part of the circumference of the flow channel contacts the porous plate.

5. The cold trap according to claim 4, wherein A heat insulation plate is provided outside the cold trap box, and a part of the circumference of the flow channel contacts the porous plate, and another part contacts the heat insulation plate.

6. The cold trap according to any one of claims 1-5, characterized in that, The porous plate is a porous plate capable of adsorbing liquid nitrogen.

7. The cold trap according to claim 6, wherein The cross-sectional area of the flow channel is in the range of 0.7 mm 2 to 314.0 mm 2 The cross-sectional area of the pore channels of the porous plate is in the range of 0.19×10 -6 mm 2 to 0.2 mm 2 and the cross-sectional area of the flow channel / the cross-sectional area of the pore channels of the porous plate ≥ 10.

8. The cold trap according to any one of claims 1-5, characterized in that, The lower box body wall is further provided with a first sampling port for taking out the sample in the receiving cavity.

9. The cold trap according to any one of claims 1-5, characterized in that, The upper box body wall is provided with a liquid injection hole for injecting a refrigerant, and the liquid injection hole communicates with the cavity.

10. An access device, characterized in that, It includes a lifting mechanism, a top plate and the cold trap according to any one of claims 1 - 8, the top plate being covered on the upper end of the cold trap box, the opening being located at the lower end of the cold trap box, the lifting mechanism being provided on the top plate and at least partially located in the receiving cavity, and the lifting mechanism being configured to take the sample into or out of the cold trap box.

11. The access device according to claim 10, wherein, The lifting mechanism includes a lifting part and an extraction part, the lifting part being provided on the top plate, the extraction part being partially located in the receiving cavity, the lifting part being drivingly connected to the extraction part for taking the sample into or out of the cold trap box through the extraction part.

12. The access device according to claim 11, characterized in that, The extraction part includes a vertically extending conductive sheet, a magnetic head with an electromagnet and a conductive contact rod electrically connected to the electromagnet, the conductive sheet being provided on the cold trap box, the conductive contact rod being slidably provided on the conductive sheet and being in electrical contact with the conductive sheet, and the lifting part being drivingly connected to the magnetic head to vertically slide the conductive contact rod on the conductive sheet.

13. The access device according to claim 11, wherein Vertically extending guide rails are connected to two opposite sides of the cold trap box, and both sides of the extraction part are slidably provided on the guide rails.

14. The access device according to claim 13, wherein The guide rail has two vertically extending tracks, at least part of which overlap in the height direction. A first slider is slidably connected to one of the tracks, and the first slider is fixedly connected to the cold trap box. A second slider is slidably connected to the other track, and the second slider is fixedly connected to the extraction part.

15. The access device according to claim 11, wherein The lifting part includes a belt and a driving motor installed on the top plate. The extraction part is fixedly connected to the belt, and the driving motor is drivingly connected to the belt to move the extraction part fixed on the belt up and down.

16. The access device according to claim 15, characterized in that, A first shield is further provided on one side of the top plate away from the cold trap box. The top plate is provided with a through hole for the belt to pass through; the driving motor is installed in the space enclosed by the top plate and the first shield.

17. The access device according to claim 11, wherein, A first roller is provided on one side of the cold trap box, and a second roller is provided on the other opposite side; the first roller and the second roller are used to contact the basket pulled by the extraction part from two opposite sides.

18. The access device according to claim 17, wherein The first roller is connected with a telescopic member, and the telescopic member is used to apply a force to the first roller towards the side where the second roller is located.

19. The access device according to claim 18, characterized in that, The cold trap box is provided with a roller frame, and the roller frame is provided with a chute extending from the side where the first roller is located to the side where the second roller is located; the first roller is slidably arranged in the chute; the telescopic member is arranged between the roller frame and the first roller.

20. A biological sample storage system, characterized in that, It includes a frame, a storage tank, and the access device according to any one of claims 10 - 19. The storage tank is provided with a second sampling port, and a basket for placing samples is arranged in the storage tank; the cold trap is configured to be movably arranged on the frame so that the opening is aligned with the second sampling port; the lifting mechanism is configured to enter the storage tank from the second sampling port and pull the basket into the cold trap box.

21. The biological sample storage system according to claim 20, characterized in that, Two opposite sides of the basket are metal plates; the metal plates are provided with guide grooves penetrating up and down. A first roller is provided on one inner side of the cold trap box, and a second roller is provided on the other opposite inner side; when the lifting mechanism controls the lifting of the basket, the first roller and the second roller slide in the guide grooves respectively.

22. The biological sample storage system according to claim 21, wherein, The biological sample storage system further includes a first sampling mechanism. The lower box body wall is further provided with a first sampling port. The first sampling mechanism includes a first sampling part and a shovel plate. The first sampling part is drivingly connected to the shovel plate, and the first sampling part is configured to move the shovel plate to the first sampling port to take out the sample box on the basket in the accommodating cavity through the shovel plate.

23. The biological sample storage system according to claim 22, characterized in that, The biological sample storage system further includes a second sampling mechanism. The second sampling mechanism includes a second sampling part, a sample box sampling clip, and a sample tube sampling clip. The second sampling part is connected to the sample box sampling clip to move the sample box, or the second sampling part is connected to the sample tube sampling clip to move the sample tube in the sample box.

24. The biological sample storage system according to claim 23, wherein, The second sampling part is provided with an electromagnet, and the second sampling part is connected to the sample box sampling clip or the sample tube sampling clip by magnetic attraction.

25. The biological sample storage system according to any one of claims 20-24, characterized in that, The frame has a liquid nitrogen adding station, and a liquid adding pipe is arranged at the liquid nitrogen adding station; a liquid injection hole for injecting liquid nitrogen is arranged on the wall of the upper box body; further included is a moving mechanism, which is drivingly connected to the cold trap box to transfer the cold trap box to the liquid nitrogen adding station, and the liquid adding pipe can be selectively inserted into the liquid injection hole to inject liquid nitrogen into the cavity.

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