Cell culture plate and cell culture device comprising same
By designing the skirt structure and limiting base on the cell culture plate, the problem of hepatocytes flowing out before they adhere to the wall is solved, the cell adherence effect and material exchange ability are enhanced, and the cell activity and functionality are improved.
Patent Information
- Application Number
- CN202422269563.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-09-14
AI Technical Summary
In the prior art, hepatocytes tend to flow out of the space between cell culture plates before adhering to the wall, resulting in a decrease in activity and functionality.
A cell culture plate is designed, including a skirt structure and a limiting base. The upper surface of the skirt structure protrudes from the upper surface of the main body, with an angle of 90°-180°. The limiting base protrudes from the surface of the cell culture plate to ensure the spacing between adjacent cell culture plates, avoid contact with the skirt structure, and enhance the adhesion effect of cells.
It effectively avoids premature outflow of liver cells, enhances the cell's adherence ability, ensures the adequacy of substance exchange, and improves the activity and functionality of cells.
Smart Images
Figure CN223176123U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of cell culture devices, and particularly relates to a cell culture plate and a cell culture device including the same. Background Art
[0002] The bioartificial liver contains functional hepatocytes and has the functions of biosynthesis and secretion metabolism. It is the artificial liver support system closest to the normal liver. The bioartificial liver (BAL) support system places or cultures the cultured source hepatocytes in a cell culture device. The patient's blood or plasma flows through the reactor through a dialysis circulation device, and then exchanges substances with the cultured hepatocytes through a semipermeable membrane or direct contact, and assists in the treatment of liver failure and related liver diseases. The BAL not only has the function of removing endotoxins in the liver, but also has functions such as participating in biosynthesis, biotransformation, and secreting active substances that promote hepatocyte growth, so as to achieve a temporary support effect on the patient.
[0003] In order to provide the activity and functionality of the source hepatocytes, when culturing hepatocytes in a cell culture device, it is necessary to make the hepatocytes adhere to the wall as much as possible, or in a cell culture device including multiple cell culture plates, make the hepatocytes stay in the space between the cell culture plates as much as possible before adhering. However, in the current culture technology, it often occurs that the hepatocytes flow out of the space between the cell culture plates before adhering and are distributed to the gap between the cell culture plate and the inner wall of the housing of the cell culture device. In this way, the activity and functionality of the obtained hepatocytes are both reduced. Summary of the Utility Model
[0004] The technical problem to be solved by the utility model is to overcome the defect that cells in the prior art flow out of the space between the cell culture plates prematurely, and provide a cell culture plate and a cell culture device including the same.
[0005] The utility model solves the above technical problems through the following technical solutions:
[0006] A cell culture plate, which includes a main body, a skirt structure and a limiting base;
[0007] The skirt structure is disposed around the circumference of the main body. The upper surface of the skirt structure protrudes from the upper surface of the main body. A preset angle is formed between the skirt structure and the main body, and the angle range of the preset angle is 90° - 180°;
[0008] The surface of the limiting base protrudes from the surface of the cell culture plate, and the height difference between the surface of the limiting base and the surface of the cell culture plate is greater than the height difference between the upper surface of the skirt structure and the upper surface of the main body.
[0009] In this technical solution, by providing this cell culture plate, the upper surface of the skirt structure protrudes from the upper surface of the main body, which can enable the cells to stay in the space between adjacent cell culture plates before firmly adhering, avoiding premature outflow of the cells from the space between the cell culture plates, resulting in reduced activity and functionality. And a preset angle is formed between the skirt structure and the main body, and the angle range of the preset angle is 90°-180°, which can avoid the situation that the cells are stabilized in an acute-angled gap and it is difficult to carry out sufficient mass exchange with the fluid flowing through the cell culture plate. The limiting base can abut against the surface of adjacent cell culture plates to ensure the spacing between adjacent cell culture plates and avoid contact between the skirt structures of adjacent cell culture plates, resulting in the situation that the cells at the skirt are difficult to carry out sufficient mass exchange with the fluid flowing through the cell culture plate.
[0010] Preferably, the horizontal width range of the skirt structure is 1.5 mm - 5 mm.
[0011] In this technical solution, through the above settings, while limiting the cells, it is possible to avoid the skirt structure being too wide and disturbing the fluid flowing through the cell culture plate.
[0012] Preferably, the ratio of the thickness of the skirt structure to the thickness of the main body ranges from 1 / 4 - 1; and / or,
[0013] The thickness range of the main body is 1 mm - 2 mm, and the thickness range of the skirt structure is 0.5 mm - 1 mm.
[0014] In this technical solution, through the above settings, while the strengths of the skirt structure and the main body are reasonable, the overall weight of the cell culture plate can be appropriately reduced.
[0015] Preferably, the skirt structure and the main body are integrally formed; and / or,
[0016] The skirt structure is smoothly connected to the main body.
[0017] In this technical solution, by setting the skirt structure and the main body to be integrally formed, stress concentration at the connection between the skirt structure and the main body can be avoided, reducing the service life of the cell culture plate. By setting the skirt structure to be smoothly connected to the main body, damage to the cells caused by the connection between the skirt structure and the main body can be avoided, and the impurities contained in the fluid flowing through the cell culture plate can be reduced.
[0018] Preferably, the outer shape of the end face of the skirt structure away from the main body is a smooth rounded shape.
[0019] In this technical solution, through the above settings, damage to the cells caused by the skirt structure can be avoided, and the impurities contained in the fluid flowing through the cell culture plate can be reduced.
[0020] Preferably, the material of the cell culture plate is polystyrene.
[0021] In this technical solution, through the above settings, it is further beneficial to the adherent culture of cells.
[0022] A cell culture device includes a plurality of cell culture plates as described above.
[0023] In this technical solution, by providing this cell culture device, it is convenient for adherent culture of cells and for the cells to stay in the space between the cell culture plates before adhesion.
[0024] Preferably, the cell culture device further includes a culture container, and a plurality of the cell culture plates are placed side by side in the inner cavity of the culture container;
[0025] The distance between adjacent cell culture plates ranges from 0.5 mm to 1 mm.
[0026] In this technical solution, through the above settings, it is further convenient for the cells to stay in the space between the cell culture plates before adhesion.
[0027] Preferably, the cross-sectional shape of the culture container corresponds to the cross-sectional shape of the cell culture plate.
[0028] In this technical solution, through the above settings, it is further convenient for the cells to stay in the space between the cell culture plates before adhesion, and reduce the situation that the cells flow out into the gap between the culture container and the cell culture plate.
[0029] The positive and progressive effects of the present utility model are as follows:
[0030] By providing this cell culture plate, the upper surface of the skirt structure protrudes from the upper surface of the main body, so that the cells can stay in the space between adjacent cell culture plates before firmly adhering, avoiding the premature outflow of the cells from the space between the cell culture plates, resulting in reduced activity and functionality; and a preset angle is formed between the skirt structure and the main body, and the angle range of the preset angle is 90°-180°, which can avoid the situation that the cells are stable in an acute-angle-shaped gap and it is difficult to perform sufficient mass exchange with the fluid flowing through the cell culture plate. By providing this cell culture device, it is convenient for adherent culture of cells and for the cells to stay in the space between the cell culture plates before adhesion. The limiting base can abut against the surface of adjacent cell culture plates to ensure the distance between adjacent cell culture plates and avoid the contact of the skirt structures of adjacent cell culture plates, resulting in the situation that the cells at the skirt are difficult to perform sufficient mass exchange with the fluid flowing through the cell culture plate. Description of the Drawings
[0031] Figure 1Schematic diagram of the positional relationship between the main body and the skirt structure according to an embodiment of the present utility model.
[0032] Figure 2 Schematic diagram of the positional relationship of a cell culture plate according to an embodiment of the present utility model.
[0033] Figure 3 Schematic cross-sectional structure diagram of a cell culture device according to an embodiment of the present utility model.
[0034] Figure 4 is Figure 3 Partial enlarged view of A of
[0035] Explanation of reference numerals:
[0036] Cell culture plate 1
[0037] Main body 11
[0038] Skirt structure 12
[0039] Limit base 13
[0040] Culture container 2 Detailed implementation manners
[0041] The following will more clearly and completely illustrate the present utility model in conjunction with embodiments and the accompanying drawings.
[0042] As Figure 1 、 Figure 2 The embodiment shown provides a cell culture plate 1, which includes a main body 11 and a skirt structure 12. The skirt structure 12 is disposed around the circumference of the main body 11, and the upper surface of the skirt structure 12 protrudes from the upper surface of the main body 11. In this way, cells can stay in the space between adjacent cell culture plates 1 before firmly adhering, avoiding premature outflow of cells from the space between the cell culture plates 1, resulting in reduced activity and functionality. A preset angle α is formed between the skirt structure 12 and the main body 11. When α = 160°, it can avoid the situation where cells are stabilized in an acute-angled gap and it is difficult to perform sufficient mass exchange with the fluid flowing through the cell culture plate 1. In other embodiments, the angle of the preset angle can also take other values within the range of 90° - 180° to achieve a similar effect.
[0043] Please refer to Figure 3, in this embodiment, the cell culture plate 1 further includes a limiting base 13. The surface of the limiting base 13 protrudes from the surface of the cell culture plate 1, and the height difference between the surface of the limiting base 13 and the surface of the cell culture plate 1 is greater than the height difference between the upper surface of the skirt structure 12 and the upper surface of the main body 11. In this way, the limiting base 13 can abut against the surface of an adjacent cell culture plate 1 to ensure the spacing between adjacent cell culture plates 1 and avoid contact between the skirt structures 12 of adjacent cell culture plates 1, which may cause the cells at the skirt to be difficult to fully exchange substances with the fluid flowing through the cell culture plate 1.
[0044] In this embodiment, the horizontal width W of the skirt structure 12 is 2.5 mm. In this way, while limiting the cells, it is possible to avoid the skirt structure 12 being too wide and disturbing the fluid flowing through the cell culture plate 1. In other embodiments, the horizontal width of the skirt structure can also take other values within the range of 1.5 mm - 5 mm to achieve a similar effect.
[0045] In this embodiment, the thickness D1 of the skirt structure 12 is 0.75 mm, and the thickness D2 of the main body 11 is 1.5 mm, and D1:D2 = 1:2. In this way, while the strengths of the skirt structure 12 and the main body 11 are reasonable, the overall weight of the cell culture plate 1 can be appropriately reduced. In other embodiments, the thickness of the skirt structure can also take other values within the range of 0.5 mm - 1 mm, the thickness of the main body can also take other values within the range of 1 mm - 2 mm, or the ratio of the thickness of the skirt structure to the thickness of the main body can take other values within the range of 1 / 4 - 1 to achieve a similar effect.
[0046] In this embodiment, by setting the skirt structure 12 and the main body 11 to be integrally formed, stress concentration at the connection between the skirt structure 12 and the main body 11 can be avoided, and the lifespan of the cell culture plate 1 can be reduced.
[0047] In this embodiment, by setting the skirt structure 12 to be smoothly connected to the main body 11 (the skirt structure 12 and the main body 11 are connected by an arc with a radius R = 2 mm), damage to the cells at the connection between the skirt structure 12 and the main body 11 can be avoided, and the impurities contained in the fluid flowing through the cell culture plate 1 can be reduced. Here, "smoothly connected" means that the surface at the connection has no visible bumps, seams, breaks, etc. to the naked eye.
[0048] In this embodiment, further, the outer shape of the end face of the skirt structure 12 away from the main body 11 is in the shape of a smooth rounded corner. In this way, damage to the cells caused by the skirt structure 12 can be avoided, and the impurities contained in the fluid flowing through the cell culture plate 1 can be reduced.
[0049] In this embodiment, the material of the cell culture plate 1 is PS (polystyrene). In this way, it is further beneficial to the adherent culture of cells. In other embodiments, the material of the cell culture plate can also be other polymer materials suitable for cell adhesion.
[0050] As Figure 3 , Figure 4 shown, this embodiment also provides a cell culture device, which includes multiple cell culture plates 1 as described above. The multiple cell culture plates 1 are stacked in the height direction to accommodate more cells. At the same time, the structure of the above-mentioned cell culture plate 1 can also facilitate the adherent culture of cells and staying in the space between the cell culture plates 1 before adhesion.
[0051] Please refer back to Figure 2 , Figure 4 , in this embodiment, the cell culture device further includes a culture container 2. These cell culture plates 1 are placed together in the inner cavity of the culture container 2. The distance H in the vertical direction between adjacent cell culture plates 1 is 0.75 mm. In this way, it is further convenient for cells to stay in the space between the cell culture plates 1 before adhesion. In other embodiments, the distance between adjacent cell culture plates can also take other values within the range of 0.5 mm - 1 mm to achieve a similar effect.
[0052] In this embodiment, the cross-sectional shape of the culture container 2 and the cross-sectional shape of the cell culture plate 1 are both circular. In this way, it is further convenient for cells to stay in the space between the cell culture plates 1 before adhesion, reducing the situation where cells flow out into the gap between the culture container 2 and the cell culture plate 1. In other embodiments, the cross-sectional shape of the culture container and the cross-sectional shape of the cell culture plate can be adjusted according to requirements, but it is best to correspond to each other to reduce cell outflow. [[ID=]]
[0053] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example. The protection scope of the present invention is defined by the appended claims. Without departing from the principles and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.
Claims
1. A cell culture plate, characterized in that, It includes a main body, a skirt structure and a limiting base; The skirt structure is arranged around the circumference of the main body. The upper surface of the skirt structure protrudes from the upper surface of the main body. A preset angle is formed between the skirt structure and the main body, and the angle range of the preset angle is 90°-180°; The surface of the limiting base protrudes from the surface of the cell culture plate, and the height difference between the surface of the limiting base and the surface of the cell culture plate is greater than the height difference between the upper surface of the skirt structure and the upper surface of the main body.
2. The cell culture plate according to claim 1, wherein The horizontal width range of the skirt structure is 1.5 mm - 5 mm.
3. The cell culture plate according to claim 1, wherein The ratio range of the thickness of the skirt structure to the thickness of the main body is 1 / 4 - 1; and / or, The thickness range of the main body is 1 mm - 2 mm, and the thickness range of the skirt structure is 0.5 mm - 1 mm.
4. The cell culture plate according to claim 1, wherein The skirt structure and the main body are integrally formed.
5. The cell culture plate according to claim 1, wherein The skirt structure is smoothly connected to the main body.
6. The cell culture plate according to claim 1, wherein The outer shape of the end face of the skirt structure away from the main body is a smooth rounded shape.
7. The cell culture plate according to claim 1, wherein The material of the cell culture plate is polystyrene.
8. A cell culture device, characterized in that, It includes multiple cell culture plates as described in any one of claims 1-7.
9. The cell culture device according to claim 8, wherein, The cell culture device further includes a culture container, and multiple cell culture plates are placed side by side in the inner cavity of the culture container; The distance range between adjacent cell culture plates is 0.5 mm - 1 mm.
10. The cell culture device according to claim 9, wherein, The cross-sectional shape of the culture container corresponds to the cross-sectional shape of the cell culture plate.