Production system for separating ergothioneine from natural rubber whey
By designing a production system including an acidifying kettle, a filtration device, an ion resin column, a concentration device and a drying device, the problem of low separation efficiency of ergothion in natural rubber wax was solved, and ergothion extraction with high yield, high purity and high yield was achieved.
Patent Information
- Application Number
- CN202422094444.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-28
- Publication Date
- 2025-08-05
- Estimated Expiration
- 2034-08-28
AI Technical Summary
The prior art is difficult to efficiently separate ergothione from natural rubber wax, resulting in high production costs and low yield and purity.
A production system is designed, including an acidifying kettle, a filtration device, an ion resin column, a concentration device, a crystallization kettle and a drying device, and ergothioneine is extracted from natural rubber wax through a series of physical and chemical treatment steps, including acidification, filtration, neutralization, concentration, crystallization and drying processes.
The separation of ergothione from natural rubber wax is achieved with high yield, high purity and high yield, reducing production costs and improving product quality and efficiency.
Smart Images
Figure CN223184540U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of thioneine production, in particular to a production system for separating thioneine from natural rubber whey. Background Art
[0002] L-ergothioneine (EGT) is a natural amino acid strong antioxidant with the chemical formula C9H 15 N₃O₂S exhibits numerous biological activities, including antioxidant activity, DNA biosynthesis maintenance, normal cell growth, and cellular immunity. It is also used in high-end cosmetics for its anti-radiation, whitening, and anti-aging properties. Since the discovery of the EGT transporter (ETT / OCTN1) in 2005, EGT has become a research hotspot, and reports on this compound have increased exponentially. Currently, industrial production of EGT primarily relies on biosynthesis. The rapid development of biosynthesis has greatly accelerated research into EGT production through microbial fermentation. The highest publicly reported yield of EGT production is 1.63±0.04 g / L after 220 hours of fermentation. However, due to limitations in fermentation equipment and cycle time, scaling up production is costly.
[0003] The rubber tree (Hevea bras il iens is) is an important economic tree in tropical regions. The natural rubber latex it produces is an emulsified aqueous dispersion composed of approximately 35% rubber hydrocarbon particles and 65% whey. In 1968, TAN et al. first discovered EGT in natural rubber latex. Because it is synthesized by microorganisms in the soil, it is greatly affected by the season and the rubber plantation environment. The content of EGT in natural rubber whey (NRS) is usually 10-15 mg / L, with a maximum of 113 mg / L and a minimum of 2.7 mg / L. my country's natural rubber planting area is over 1.1333 million hectares. 2 The dry rubber output is about 800,000 tons, and the treatment cost of NRS as a highly concentrated organic wastewater is high. Therefore, in view of the existing technical problems, it is necessary to provide a production system for separating thioneine from natural rubber whey. Utility Model Content
[0004] The technical problem to be solved by the utility model is: in view of the deficiencies in the prior art, a production system for separating thioneine from natural rubber whey is provided, and a high-purity, high-yield and high-yield thioneine product can be obtained by utilizing the production system.
[0005] In order to solve the above technical problems, the technical solution of the utility model is:
[0006] A production system for separating thioneine from natural rubber whey comprises an acidification kettle connected to a latex conveying pipeline, a discharge port of the acidification kettle connected to a first filter, a liquid outlet of the first filter connected to a neutralization kettle, a discharge port of the neutralization kettle connected to a second filter, a liquid outlet of the second filter connected to a cationic resin column, a liquid outlet of the cationic resin column connected to an anionic resin column, a liquid outlet of the anionic resin column connected to a first concentrating device, a concentrated liquid outlet of the first concentrating device connected to a silica gel column, a parsed liquid outlet of the silica gel column connected to a second concentrating device, a liquid outlet of the second concentrating device connected to a crystallization kettle, a discharge port of the crystallization kettle connected to a washing kettle, a discharge port of the washing kettle connected to a drying device, and a discharge port of the drying device connected to a product storage tank.
[0007] As an improved technical solution, the acidification kettle includes a kettle body, a latex inlet and an organic acid inlet are provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a plurality of stirring plates are provided on the stirring shaft, and a plurality of stirring rods are provided on the stirring plate.
[0008] As an improved technical solution, the first filtering device and the second filtering device are both plate and frame filters.
[0009] As an improved technical solution, the neutralization kettle includes a kettle body, the top of the kettle body is provided with a feed inlet and a neutralizer inlet, the bottom of the kettle body is provided with a discharge port, the outside of the kettle body is provided with a jacket, the inside of the kettle body is provided with a stirring shaft, one end of the stirring shaft is connected to a motor, and the stirring shaft is provided with multiple stirring plates with a hollow structure.
[0010] As an improved technical solution, the first concentrating device is a nanofiltration membrane device.
[0011] As an improved technical solution, the second concentrating device includes a kettle body, a feed port and an exhaust port are provided on the top of the kettle body, a concentrated liquid outlet is provided at the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a stirring frame is provided on the stirring shaft, a plurality of stirring blocks are provided on the stirring frame, and a stirring rod is provided between two adjacent stirring blocks.
[0012] As an improved technical solution, the crystallization kettle includes a kettle body, the top and bottom of the kettle body are respectively provided with a feed port and a discharge port, the outside of the kettle body is provided with a jacket, the inside of the kettle body is provided with a stirring shaft, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft. A liquid outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet.
[0013] As an improved technical solution, the washing kettle includes a kettle body, a feed port and a washing liquid inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a plurality of fan-shaped stirring blades are provided on the stirring shaft, a washing liquid outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the washing liquid outlet.
[0014] After adopting the above technical solution, the beneficial effects of the utility model are:
[0015] The invention discloses a production system for separating thioneine from natural rubber whey, comprising an acidifying kettle connected to a latex conveying pipeline, wherein the discharge port of the acidifying kettle is connected to a first filter, the liquid outlet of the first filter is connected to a neutralization kettle, the discharge port of the neutralization kettle is connected to a second filter, the liquid outlet of the second filter is connected to a cationic resin column, the liquid outlet of the cationic resin column is connected to an anionic resin column, the liquid outlet of the anionic resin column is connected to a first concentrating device, the concentrated liquid outlet of the first concentrating device is connected to a silica gel column, the analytical liquid outlet of the silica gel column is connected to a second concentrating device, the liquid outlet of the second concentrating device is connected to a crystallization kettle, the discharge port of the crystallization kettle is connected to a washing kettle, the discharge port of the washing kettle is connected to a drying device, and the discharge port of the drying device is connected to a product storage tank. In actual production, the fresh latex collected enters the inside of acidifying kettle along latex delivery pipeline, adds organic acid, then filters through the first filtering device, the filtrate collected enters the inside of neutralization kettle again, adds neutralizing agent after neutralization reaction, then filters through the second filtering device, the filtrate collected enters cationic resin column, the effluent after removing cation enters anionic resin column again, the effluent after removing anion enters the first concentrating device again, the concentrated solution after hot concentration enters silica gel column again, thioneine adopts analytical agent to parse after being adsorbed, the analytical solution collected enters the second concentrating device again, the concentrated solution collected enters crystallization kettle again, the crystal after decrease temperature crystallization enters washing kettle again, the crystal after washing passes through the drying of drying device again, and the thioneine product obtained adopts product storage tank to store. Above-mentioned production system is reasonable in design, is convenient to separate the thioneine product obtaining high yield, high purity and high yield from natural rubber whey.
[0016] The acidification kettle comprises a kettle body, with latex and organic acid inlets located at the top, a discharge port at the bottom, and a stirring shaft mounted inside. One end of the stirring shaft is connected to a motor, and the stirring shaft is provided with multiple stirring plates, each of which is provided with multiple stirring rods. In actual production, fresh latex and organic acid are separately introduced into the kettle body. Once the motor is activated, it drives the stirring shaft, multiple stirring plates, and multiple stirring rods to rotate, ensuring that the organic acid and fresh latex are fully contacted and mixed, reducing the activity of protein enzymes such as rubber extension factor and promoting the rapid aggregation of rubber hydrocarbon particles in the latex. The acidified latex is then discharged from the discharge port and entered into the next processing equipment.
[0017] Since both the first filter device and the second filter device are plate-and-frame filters, the first filter device can effectively remove rubber hydrocarbon particles aggregated in the latex, while the second filter device can effectively remove rubber particle coagulation and protein coagulation.
[0018] The neutralization kettle comprises a kettle body with a feed inlet and a neutralizer inlet at the top, a discharge port at the bottom, a jacket on the outside, and a stirring shaft mounted inside. One end of the stirring shaft is connected to a motor, and the stirring shaft is equipped with multiple hollow stirring plates. In actual production, the filtrate and neutralizer enter the kettle separately. The heat medium in the jacket is used to provide the required temperature for the neutralization reaction. The motor is then activated to drive the stirring shaft and multiple hollow stirring plates to fully mix the neutralizer and filtrate, promoting a full reaction between the feed and neutralizer, thereby promoting the coagulation of the rubber particles and the aggregation of the protein.
[0019] Because the first concentrating device is a nanofiltration membrane device. The effluent is passed through a nanofiltration membrane device with a pore size of 150-300Da, which can achieve the effective concentration of thioneine in the effluent.
[0020] The second concentrator includes a kettle with a feed inlet and an exhaust port at the top, a concentrate outlet at the bottom, a jacket on the outside, and a stirring shaft inside. One end of the stirring shaft is connected to a motor, and a stirring frame is mounted on the stirring shaft. The stirring frame is equipped with multiple stirring blocks, and a stirring rod is positioned between adjacent stirring blocks. In actual production, the analytical solution enters the second concentrator, and the heat medium in the jacket is used to heat the effluent. When the motor is activated, it drives the stirring shaft, stirring frame, and multiple stirring blocks to stir the effluent, ensuring uniform heating of the effluent and facilitating effective concentration of the effluent.
[0021] The crystallization kettle includes a kettle body, a feed port and a discharge port are provided at the top and bottom of the kettle body, respectively; a jacket is provided on the outside of the kettle body; a stirring shaft is provided on the inside of the kettle body; one end of the stirring shaft is connected to a motor; a plurality of stirring rods are provided on the stirring shaft; a liquid outlet is provided on one side of the lower portion of the kettle body; and a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet. In actual production, the concentrated liquid enters the kettle body and is cooled by the refrigerant in the jacket. After the motor is started, the electric stirring shaft and the plurality of stirring rods stir the concentrated liquid, causing the concentrated liquid to cool evenly and facilitate the formation of crystals; a suction pump is then used to extract the crystallization mother liquor from the liquid outlet, and the crystals are retained by the filter. The above-mentioned crystallization kettle is rationally designed and facilitates the formation of crystals.
[0022] The washing kettle comprises a kettle body, with a feed inlet and a wash liquid inlet at the top, a discharge port at the bottom, and a stirring shaft mounted inside. One end of the stirring shaft is connected to a motor and is equipped with multiple fan-shaped stirring blades. A wash liquid outlet is located on one side of the lower portion of the kettle body, and a filter is installed on the inner wall of the kettle body at a position corresponding to the wash liquid outlet. In actual production, crystals and wash liquid enter the kettle body separately. When the motor is started, it drives the stirring shaft and multiple stirring blades to rotate, ensuring that the wash liquid and crystals are fully contacted and mixed. Impurities in the crystals dissolve in the wash liquid. The wash liquid is then extracted using a suction pump, and the crystals are trapped by the filter and discharged from the discharge port. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a schematic structural diagram of a production system for separating ergothioneine from natural rubber whey according to the present invention;
[0024] Among them, 1-latex conveying pipeline, 2-acidification kettle, 3-first filtering device, 4-neutralization kettle, 5-second filtering device, 6-cationic resin column, 7-anion resin column, 8-first concentrating device, 9-silica gel column, 10-second concentrating device, 11-crystallization kettle, 12-washing kettle, 13-drying device, 14-product storage tank. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0026] A production system for separating ergothioneine from natural rubber whey, such as Figure 1 As shown, it includes an acidification kettle 2 connected to a latex conveying pipeline 1, the discharge port of the acidification kettle 2 is connected to a first filtering device 3 (plate and frame filter), the liquid outlet of the first filtering device 3 is connected to a neutralization kettle 4, the discharge port of the neutralization kettle 4 is connected to a second filtering device 5 (plate and frame filter), the liquid outlet of the second filtering device 5 is connected to a cationic resin column 6 (filler is a strong acidic cationic exchange resin), the liquid outlet of the cationic resin column 6 is connected to an anion resin column 7 (filler is a macroporous weak base anion exchange resin), and the anion resin The liquid outlet of the fat column 7 is connected to the first concentrating device 8 (nanofiltration membrane equipment with a pore size of 150-300Da), the concentrated liquid outlet of the first concentrating device 8 is connected to the silica gel column 9 (the filler is type B silica gel), the analytical liquid outlet of the silica gel column 9 is connected to the second concentrating device 10, the liquid outlet of the second concentrating device 10 is connected to the crystallization kettle 11, the discharge port of the crystallization kettle 11 is connected to the washing kettle 12, the discharge port of the washing kettle 12 is connected to the drying device 13 (double cone dryer), and the discharge port of the drying device 13 is connected to the product storage tank 14.
[0027] In actual production, the fresh latex collected enters the inside of acidifying kettle along latex transfer pipeline, add organic acid, then filter (the rubber hydrocarbon particles assembled in latex can be effectively removed) through the first filtering device, the filtrate collected enters the inside of neutralization kettle again, add neutralizing agent after neutralization reaction, then filter (rubber particles coagulant and protein coagulant can be effectively removed) through the second filtering device, the filtrate collected enters cationic resin column, the effluent after removing cation enters anionic resin column again, the effluent after removing anion enters the first concentrating device again, the concentrated solution after hot concentration enters silica gel column again, thioneine adopts analytical agent to parse after being adsorbed, the analytical solution collected enters the second concentrating device again, the concentrated solution collected enters crystallization kettle again, the crystal after decrease temperature crystalline enters washing kettle again, the crystal after washing passes through the drying of drying device again, and the thioneine product obtained adopts product storage tank to store. Above-mentioned production system is reasonable in design, is convenient to separate the thioneine product obtaining high yield, high purity and high yield from natural rubber whey.
[0028] The acidification kettle 2 includes a kettle body, with a latex inlet and an organic acid inlet at the top, a discharge port at the bottom, and a stirring shaft mounted inside the kettle. One end of the stirring shaft is connected to a motor, and the stirring shaft is provided with multiple stirring plates, each of which is provided with multiple stirring rods. In actual production, fresh latex and organic acid are separately introduced into the kettle body. When the motor is activated, it drives the stirring shaft, multiple stirring plates, and multiple stirring rods to rotate, allowing the organic acid and fresh latex to fully contact and mix, reducing the activity of protein enzymes such as rubber extension factor and promoting the rapid aggregation of rubber hydrocarbon particles in the latex. The acidified latex is then discharged from the discharge port and entered into the next processing equipment.
[0029] The neutralization kettle 4 includes a kettle body, with a feed inlet and a neutralizer inlet at the top, a discharge port at the bottom, a jacket on the outside, and a stirring shaft on the inside. One end of the stirring shaft is connected to a motor, and the stirring shaft is equipped with multiple hollow stirring plates. In actual production, the filtrate and neutralizer enter the kettle separately. The heat medium in the jacket is used to provide the required temperature for the neutralization reaction. The motor is activated to drive the stirring shaft and the multiple hollow stirring plates to fully stir and mix the neutralizer and filtrate, promoting a full reaction between the feed liquid and the neutralizer, thereby promoting the coagulation of the rubber particles and the aggregation of the protein.
[0030] The second concentrator 10 includes a kettle, with a feed inlet and an exhaust port at the top, a concentrate outlet at the bottom, a jacket on the outside of the kettle, and a stirring shaft inside the kettle. One end of the stirring shaft is connected to a motor, and the stirring shaft is provided with a stirring frame, which is provided with multiple stirring blocks, with a stirring rod located between adjacent stirring blocks. In actual production, the analytical solution enters the second concentrator, and the effluent is heated by the heat medium in the jacket. When the motor is started, it drives the stirring shaft, stirring frame, and multiple stirring blocks to stir the effluent, ensuring uniform heating of the effluent and facilitating effective concentration of the effluent.
[0031] The crystallization kettle 11 includes a kettle body, with a feed inlet and a discharge outlet provided at the top and bottom, respectively. The kettle body is provided with a jacket on the outside, and a stirring shaft is provided inside the kettle body. One end of the stirring shaft is connected to a motor, and the stirring shaft is provided with multiple stirring rods. A liquid outlet is provided on one side of the lower portion of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet. In actual production, the concentrated liquid enters the kettle body and is cooled by the refrigerant in the jacket. After the motor is started, the electric stirring shaft and multiple stirring rods stir the concentrated liquid, causing the concentrated liquid to cool evenly and facilitate the formation of crystals. A suction pump is then used to extract the crystallization mother liquor from the liquid outlet, and the crystals are trapped by the filter.
[0032] The washing kettle 12 includes a kettle body, a feed inlet and a wash liquid inlet are provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and the stirring shaft is provided with multiple fan-shaped stirring blades. A wash liquid outlet is provided on one side of the lower portion of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the wash liquid outlet. In actual production, the crystals and the wash liquid enter the interior of the kettle body separately. After the motor is started, it drives the stirring shaft and the multiple stirring blades to rotate, so that the wash liquid and crystals are fully contacted and mixed, and impurities in the crystals are dissolved in the wash liquid. The wash liquid is extracted by a suction pump, and the crystals are trapped by the filter screen and finally discharged from the discharge port.
[0033] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A production system for separating thioneine from natural rubber whey, characterized in that, The invention comprises an acidification kettle connected to a latex conveying pipeline, wherein the discharge port of the acidification kettle is connected to a first filtering device, the liquid outlet of the first filtering device is connected to a neutralization kettle, the discharge port of the neutralization kettle is connected to a second filtering device, the liquid outlet of the second filtering device is connected to a cationic resin column, the liquid outlet of the cationic resin column is connected to an anionic resin column, the liquid outlet of the anionic resin column is connected to a first concentrating device, the concentrated liquid outlet of the first concentrating device is connected to a silica gel column, the analytical liquid outlet of the silica gel column is connected to a second concentrating device, the liquid outlet of the second concentrating device is connected to a crystallization kettle, the discharge port of the crystallization kettle is connected to a washing kettle, the discharge port of the washing kettle is connected to a drying device, and the discharge port of the drying device is connected to a product storage tank.
2. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The acidification kettle includes a kettle body, a latex inlet and an organic acid inlet are provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a plurality of stirring plates are provided on the stirring shaft, and a plurality of stirring rods are provided on the stirring plate.
3. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The first filter device and the second filter device are both plate and frame filters.
4. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The neutralization kettle includes a kettle body, a feed port and a neutralizer inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring plates with hollow structures are provided on the stirring shaft.
5. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The second concentrating device is a nanofiltration membrane device.
6. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The first concentrating device includes a kettle body, a feed port and an exhaust port are provided on the top of the kettle body, a concentrated liquid outlet is provided at the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a stirring frame is provided on the stirring shaft, a plurality of stirring blocks are provided on the stirring frame, and a stirring rod is provided between two adjacent stirring blocks.
7. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The crystallization kettle includes a kettle body, the top and bottom of the kettle body are respectively provided with a feed port and a discharge port, the outside of the kettle body is provided with a jacket, the inside of the kettle body is provided with a stirring shaft, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft. A liquid outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet.
8. a kind of production system of separating thioneine from natural rubber whey according to claim 1, is characterized in that, The washing kettle includes a kettle body, a feed port and a washing liquid inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, a plurality of fan-shaped stirring blades are provided on the stirring shaft, a washing liquid outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the washing liquid outlet.