Antigen purification centrifugal device

Abstract

The utility model discloses an antigen purification centrifugal device, which relates to the technical field of centrifugal devices and comprises a barrel, a barrel cover is arranged on the upper surface of the barrel, a U-shaped handle is fixedly connected to the upper surface of the barrel cover, three supporting legs are fixedly connected to the lower surface of the barrel in an annular array mode, a motor is fixedly connected to the lower surface of the barrel, and the U-shaped handle is fixedly connected to the lower surface of the barrel. And a pipeline is fixedly connected below the left side of the outer surface of the barrel in a penetrating manner. According to the utility model, the centrifugal barrel is rotated by starting the motor, and the centrifugal barrel moves up and down under the elastic action of the spring while rotating, so that a vertical shaking force is generated, and the separation efficiency of an antigen and liquid is improved; and through the arrangement of a rhombic block and a rhombic opening II, the effect of dismounting or mounting the rhombic cylinder after use is facilitated, and through the arrangement of the structure, the centrifugal cylinder can move up and down while rotating, so that the working efficiency is improved, and the performance is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of centrifugal devices, in particular to an antigen purification centrifugal device. Background Art

[0002] Antigen purification is a key method for improving vaccine quality. This process not only increases the antigen content per unit volume of the vaccine but also enhances antigen purity and reduces immune interference from contaminants, thereby enhancing the vaccine's immune efficacy and minimizing adverse reactions. The goal of antigen purification is to separate the target protein from minor interfering impurities as closely as possible to achieve the required quality standards. During vaccine production, antigen purification generally involves two stages: primary separation and fine purification. The primary separation stage utilizes techniques such as centrifugation, sedimentation, salting out, and ultrafiltration concentration, primarily to separate cell culture fluids, concentrate the product, and remove most impurities. The fine purification stage, on the other hand, primarily utilizes high-resolution techniques such as ultracentrifugation and various chromatography methods to further ensure antigen purity and quality.

[0003] The centrifuge separates and purifies antigens by rotating at high speed. However, the existing centrifuge collects the antigens at the opening after centrifugation, and then opens the opening to discharge the antigens. However, the liquid will be discharged at the same time during the discharge, resulting in incomplete purification, and a small amount of antigens will stick to the wall and cannot be discharged; the speed of the centrifuge's high-speed rotation is also not easy to adjust. According to patent publication number CN214917113U, an antigen purification centrifuge device is involved. It includes a tank body, an inner wall arranged in the tank body, a cover body arranged on the top of the tank body, and a power device arranged at the bottom of the tank body; the inner wall is divided into a left wall and a right wall; a clamping device is arranged on the left wall and the right wall; the clamping device includes a rotating part, a first screw and a knob; the rotating shaft of the rotating part is connected to the left wall; a circular shaft is fixed on the right wall; when centrifuging, all the antigens are centrifuged to the inner wall and the cross plate, ensuring that the antigens can be completely taken out after centrifugation; the inner wall is pressed by a pressure plate and a cover body to ensure that the inner wall is easy to take out; the rotation speed can be adjusted by meshing gears of different diameters in the gear set. The following problems exist in the prior art:

[0004] In the above publication number CN214917113U, the antigen and liquid are separated by rotation, but the efficiency of separation of the antigen and liquid is low during use, making it inconvenient to use. At the same time, the antigen may adhere to the inner wall of the centrifuge cylinder after use. Utility Model Content

[0005] The utility model provides an antigen purification centrifugal device to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] An antigen purification centrifuge device includes a cylinder, a cylinder cover is provided on the upper surface of the cylinder, a U-shaped handle is fixedly connected to the upper surface of the cylinder cover, three support legs are fixedly connected in a circular array on the lower surface of the cylinder, a motor is fixedly connected to the lower surface of the cylinder, and a pipe is fixedly connected through the lower left side of the outer surface of the cylinder.

[0008] An annular groove is provided on the upper surface of the cylinder, a movable component is provided inside the cylinder, and the movable component includes a centrifugal cylinder, a plurality of strip openings communicating with the inside and outside are provided in an annular array on the outer surface of the centrifugal cylinder, and a blocking net is fixedly connected to the inside of the plurality of strip openings, a fixing ring is fixedly connected above the outer surface of the centrifugal cylinder, the outer surface of the fixing ring overlaps with the inside of the annular groove, a movable seat is fixedly connected to the middle of the lower surface of the inner wall of the centrifugal cylinder, a guide rod is movably connected to the middle of the movable seat, and a diamond-shaped opening is provided on the upper surface of the guide rod.

[0009] A further improvement of the technical solution of the present invention is that plates are fixedly connected to the left and right sides of the outer surface of the guide rod, the front of the plate is provided with a plurality of through openings communicating with each other front and back, and the side of the plate away from the guide rod overlaps the inner wall of the centrifugal cylinder.

[0010] A further improvement of the technical solution of the present utility model is that: a cylindrical seat is fixedly connected to the middle of the lower surface of the centrifugal cylinder, a diamond-shaped opening 2 is opened in the middle of the lower surface of the cylindrical seat, a circular rubber pad is fixedly connected to the lower surface of the centrifugal cylinder, a circular opening communicating with the upper and lower parts is opened in the middle of the lower surface of the circular rubber pad, and the outer surface of the cylindrical seat passes through the interior of the circular opening and is fixedly connected thereto.

[0011] A further improvement of the technical solution of the present utility model is that: the output shaft of the motor passes through the interior of the cylinder and is movably connected thereto, a diamond block is fixedly connected to the upper end of the output shaft of the motor, a fixed disk is passed through and fixedly connected above the outer surface of the output shaft of the motor, and four springs are fixedly connected to the upper surface of the fixed disk in a circular array.

[0012] A further improvement of the technical solution of the present utility model is that the upper ends of the four springs overlap with the lower surface of the circular rubber pad, and the outer surface of the diamond block is plugged into the inner part of the diamond opening 2.

[0013] A further improvement of the technical solution of the present invention is that the length of the diamond block is the same as the depth of the second diamond opening, and the shape of the fixing plate is the same as the shape of the circular rubber pad.

[0014] A further improvement of the technical solution of the present utility model is that: a connecting sleeve is fixedly connected to the lower surface of the cylinder cover, the outer surface of the connecting sleeve is threadedly connected to the upper inner wall of the annular groove, the height of the connecting sleeve is the same as the depth of the annular groove, and the lower surface of the connecting sleeve overlaps the upper surface of the fixing ring.

[0015] A further improvement of the technical solution of the present utility model is that: a diamond-shaped insertion rod is fixedly connected to the middle part of the lower surface of the cylinder cover, and the outer surface of the diamond-shaped insertion rod is plugged into the inner part of the diamond-shaped opening.

[0016] Due to the adoption of the above technical solution, the present invention has achieved the following technical advancements compared to the prior art:

[0017] 1. The utility model provides an antigen purification centrifugal device, which adopts the mutual cooperation of a cylindrical seat, a circular rubber pad, a diamond-shaped opening 2, a diamond block, a fixed disk, a spring, a centrifugal cylinder, a blocking net, and a fixed ring. The centrifugal cylinder is rotated by starting the motor, and at the same time, the centrifugal cylinder moves up and down due to the elastic action of the spring, thereby generating an up and down shaking force, thereby improving the separation efficiency of the antigen and the liquid. The arrangement of the diamond block and the diamond-shaped opening 2 facilitates the disassembly or installation of the diamond cylinder after use. The arrangement of this structure enables the centrifugal cylinder to move up and down while rotating, thereby improving work efficiency and improving performance.

[0018] 2. The utility model provides an antigen purification centrifuge device, which adopts the mutual cooperation of a movable seat, a guide rod, a diamond-shaped opening, a plate, a through opening, a cylinder cover, a connecting sleeve, and a diamond-shaped plug rod. The diamond-shaped plug rod and the diamond-shaped opening are arranged during use to facilitate the restriction of the position of the guide rod. Due to the arrangement of the movable seat, the position of the guide rod remains unchanged when the centrifuge cylinder rotates. The arrangement of the plate facilitates the scraping of antigens adhered to the inner wall of the centrifuge cylinder. The arrangement of the connecting sleeve facilitates the connection and fixation of the cylinder cover and the positioning of the centrifuge cylinder, preventing the phenomenon of excessive up and down movement. The arrangement of this structure allows the centrifuge cylinder to be scraped when it is rotated, and at the same time, there is a certain degree of upward stirring phenomenon, which accelerates the separation effect of the antigen and the liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural diagram of the utility model;

[0020] Figure 2 This is a schematic diagram of the cross-sectional structure of the cylinder of the present utility model;

[0021] Figure 3 This is a schematic diagram of the decomposition structure of the active components of the present utility model;

[0022] Figure 4This is a schematic diagram of the exploded structure of the centrifugal drum bottom and motor of the present invention;

[0023] Figure 5 This is a schematic diagram of the cylinder cover structure of the present utility model.

[0024] In the figure: 1. Cylinder body; 11. Movable assembly; 111. Centrifugal cylinder; 112. Blocking net; 113. Fixed ring; 114. Movable seat; 115. Guide rod; 116. Diamond-shaped opening 1; 117. Column seat; 118. Round rubber pad; 119. Diamond-shaped opening 2; 12. Annular groove; 2. Cylinder cover; 21. Connecting sleeve; 22. Diamond-shaped plug rod; 3. U-shaped handle; 4. Support leg; 5. Motor; 51. Diamond block; 52. Fixed plate; 53. Spring; 6. Pipeline. DETAILED DESCRIPTION

[0025] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0026] like Figure 1 As shown, the utility model provides an antigen purification centrifugal device, including a cylinder 1, a cylinder cover 2 is provided on the upper surface of the cylinder 1, a U-shaped handle 3 is fixedly connected to the upper surface of the cylinder cover 2, three support legs 4 are fixedly connected to the lower surface of the cylinder 1 in an annular array, a motor 5 is fixedly connected to the lower surface of the cylinder 1, and a pipe 6 is fixedly connected through the lower left side of the outer surface of the cylinder 1;

[0027] The arrangement of the pipe 6 facilitates the discharge of the liquid, and the arrangement of the motor 5 facilitates the movement of the internal structure of the cylinder 1, wherein the motor 5 is electrically connected via wires and a power source.

[0028] like Figure 2 - Figure 3 As shown, an annular groove 12 is provided on the upper surface of the cylinder 1, and a movable component 11 is provided inside the cylinder 1. The movable component 11 includes a centrifugal cylinder 111, and a plurality of strip-shaped openings communicating with each other inside and outside are provided in an annular array on the outer surface of the centrifugal cylinder 111. The interior of the plurality of strip-shaped openings is fixedly connected with a blocking net 112. A fixing ring 113 is fixedly connected above the outer surface of the centrifugal cylinder 111, and the outer surface of the fixing ring 113 overlaps with the interior of the annular groove 12. A movable seat 114 is fixedly connected to the middle part of the lower surface of the inner wall of the centrifugal cylinder 111, and a guide rod 115 is movably connected to the middle part of the movable seat 114. A diamond-shaped opening 116 is provided on the upper surface of the guide rod 115. Plates are fixedly connected to the left and right sides of the outer surface of the guide rod 115. A plurality of through openings communicating with each other front and back are provided on the front of the plate, and the side of the plate away from the guide rod 115 overlaps with the inner wall of the centrifugal cylinder 111.

[0029] The motor 5 is started to drive the centrifuge drum 111 to rotate. Since the centrifuge drum 111 rotates at a high speed, the antigen is separated from the liquid. Due to the arrangement of the strip-shaped opening and the blocking net 112, the ejected liquid enters the interior of the cylinder body 1 and is discharged through the pipe 6. When the centrifuge drum 111 rotates, the arrangement of the structure in the cylinder cover 2 positions the guide rod 115. Due to the arrangement of the movable seat 114, the position of the guide rod 115 remains unchanged after the centrifuge drum 111 rotates. When the centrifuge drum 111 rotates, the arrangement of the plates in the guide rod 115 facilitates the scraping of the antigens adhered to the inner wall of the centrifuge drum 111. During the scraping, the arrangement of the plates causes a certain amount of stirring inside the centrifuge drum 111, thereby accelerating the separation of the antigen from the liquid.

[0030] like Figure 4 As shown, a cylindrical seat 117 is fixedly connected to the middle part of the lower surface of the centrifugal cylinder 111, and a diamond-shaped opening 119 is opened in the middle part of the lower surface of the cylindrical seat 117. A circular rubber pad 118 is fixedly connected to the lower surface of the centrifugal cylinder 111, and a circular opening communicating with each other is opened in the middle part of the lower surface of the circular rubber pad 118. The outer surface of the cylindrical seat 117 passes through the interior of the circular opening and is fixedly connected thereto. The output shaft of the motor 5 passes through the interior of the cylinder body 1 and is movably connected thereto. The upper end of the output shaft of the motor 5 is fixedly connected to a diamond block 51, and a fixed disk 52 is fixedly connected to the upper end of the outer surface of the output shaft of the motor 5. Four springs 53 are fixedly connected to the upper surface of the fixed disk 52 in an annular array. The upper ends of the four springs 53 overlap with the lower surface of the circular rubber pad 118, and the outer surface of the diamond block 51 is plugged into the interior of the diamond opening 119. The length of the diamond block 51 is the same as the depth of the diamond opening 119, and the shape of the fixed disk 52 is the same as that of the circular rubber pad 118.

[0031] When the motor 5 is started, the centrifuge barrel 111 is driven to rotate due to the arrangement of the diamond block 51 and the diamond-shaped opening 119. While rotating, the centrifuge barrel 111 moves up and down under the elastic force of the spring 53. The centrifuge barrel 111 has the characteristic of moving up and down while rotating, thereby improving the performance. At the same time, the arrangement of the diamond block 51 and the diamond-shaped opening 119 makes it convenient to directly remove the centrifuge barrel 111 from the inside of the barrel body 1 after use, so as to take out the antigen.

[0032] like Figure 5 As shown, the lower surface of the cylinder cover 2 is fixedly connected to a connecting sleeve 21, the outer surface of the connecting sleeve 21 is threadedly connected to the upper inner wall of the annular groove 12, the height of the connecting sleeve 21 is the same as the depth of the annular groove 12, the lower surface of the connecting sleeve 21 overlaps the upper surface of the fixing ring 113, and the middle part of the lower surface of the cylinder cover 2 is fixedly connected to a diamond-shaped plug rod 22, the outer surface of the diamond-shaped plug rod 22 is plugged into the inner part of the diamond-shaped opening 116;

[0033] The threaded arrangement in the connecting sleeve 21 and the annular groove 12 facilitates the installation and connection of the cylinder cover 2, and the arrangement of the diamond-shaped insert rod 22 facilitates the insertion of the guide rod 115 into the diamond-shaped opening 116 to position the guide rod 115 and facilitates installation and disassembly.

[0034] The working principle of the antigen purification centrifugal device is described in detail below.

[0035] When the motor 5 is started, the diamond block 51 and the diamond mouth 119 are set to drive the centrifuge 111 to rotate. Since the centrifuge 111 rotates at a high speed, the antigen is separated from the liquid. Due to the setting of the strip mouth and the blocking net 112, the ejected liquid enters the interior of the cylinder 1. At the same time, when the centrifuge 111 rotates, the centrifuge 111 moves up and down due to the elastic force of the spring 53. The diamond plug 22 and the diamond mouth 116 are set to facilitate positioning of the guide rod 115. At the same time, the setting of the plate facilitates the scraping of the antigen adhered to the inner wall of the centrifuge 111. While scraping, the setting of the plate causes a certain amount of stirring inside the centrifuge 111, which accelerates the separation of the antigen and the liquid, and makes the liquid discharged through the pipeline 6.

[0036] The above generally describes the present invention in detail. However, it is obvious to those skilled in the art that modifications or improvements may be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. An antigen purification centrifugal device, comprising a barrel (1), characterized in that: The upper surface of the cylinder (1) is provided with a cylinder cover (2), the upper surface of the cylinder cover (2) is fixedly connected to a U-shaped handle (3), the lower surface of the cylinder (1) is fixedly connected to three supporting legs (4) in an annular array, the lower surface of the cylinder (1) is fixedly connected to a motor (5), and a pipe (6) is fixedly connected to the lower left side of the outer surface of the cylinder (1); An annular groove (12) is provided on the upper surface of the cylinder (1), and a movable component (11) is provided inside the cylinder (1). The movable component (11) includes a centrifugal cylinder (111), and a plurality of strip-shaped openings communicating with each other are provided in an annular array on the outer surface of the centrifugal cylinder (111). The interiors of the plurality of strip-shaped openings are fixedly connected with a blocking net (112). A fixing ring (113) is fixedly connected above the outer surface of the centrifugal cylinder (111), and the outer surface of the fixing ring (113) overlaps with the interior of the annular groove (12). A movable seat (114) is fixedly connected to the middle of the lower surface of the inner wall of the centrifugal cylinder (111), and a guide rod (115) is movably connected to the middle of the movable seat (114). A diamond-shaped opening (116) is provided on the upper surface of the guide rod (115).

2. An antigen purification centrifugal device according to claim 1, characterized in that: Plates are fixedly connected to the left and right sides of the outer surface of the guide rod (115), and a plurality of openings communicating with each other are opened on the front of the plate. The side of the guide rod (115) away from the plate overlaps the inner wall of the centrifugal cylinder (111).

3. The antigen purification centrifugal device according to claim 1, characterized in that: A cylindrical seat (117) is fixedly connected to the middle of the lower surface of the centrifugal cylinder (111), and a diamond-shaped opening (119) is opened in the middle of the lower surface of the cylindrical seat (117). A circular rubber pad (118) is fixedly connected to the lower surface of the centrifugal cylinder (111), and a circular opening communicating with each other from top to bottom is opened in the middle of the lower surface of the circular rubber pad (118). The outer surface of the cylindrical seat (117) passes through the interior of the circular opening and is fixedly connected thereto.

4. The antigen purification centrifugal device according to claim 1, characterized in that: The output shaft of the motor (5) passes through the interior of the cylinder (1) and is movably connected thereto; a rhombus block (51) is fixedly connected to the upper end of the output shaft of the motor (5); a fixed disk (52) is fixedly connected to the upper surface of the output shaft of the motor (5); and four springs (53) are fixedly connected to the upper surface of the fixed disk (52) in an annular array.

5. The antigen purification centrifugal device according to claim 4, characterized in that: The upper ends of the four springs (53) overlap with the lower surface of the circular rubber pad (118), and the outer surface of the diamond block (51) is plugged into the interior of the second diamond opening (119).

6. The antigen purification centrifugal device according to claim 4, characterized in that: The length of the diamond block (51) is the same as the depth of the second diamond opening (119), and the shape of the fixing plate (52) is the same as the shape of the circular rubber pad (118).

7. The antigen purification centrifugal device according to claim 1, characterized in that: A connecting sleeve (21) is fixedly connected to the lower surface of the cylinder cover (2); the outer surface of the connecting sleeve (21) is threadedly connected to the upper inner wall of the annular groove (12); the height of the connecting sleeve (21) is the same as the depth of the annular groove (12); and the lower surface of the connecting sleeve (21) overlaps the upper surface of the fixing ring (113).

8. The antigen purification centrifugal device according to claim 2, characterized in that: A diamond-shaped inserting rod (22) is fixedly connected to the middle portion of the lower surface of the cylinder cover (2), and the outer surface of the diamond-shaped inserting rod (22) is plugged into the interior of the diamond-shaped opening (116).

Images