Manual loading instrument

Abstract

The utility model discloses a manual loading instrument and belongs to the technical field of gene sequencing. The manual loading instrument comprises a support, and the support is provided with a clamping groove and a bearing frame at intervals in the first direction; the main body is inserted into the clamping groove, the main body is provided with a containing groove extending in the first direction, and the flow cell can be placed in the containing groove; the liquid adding assembly comprises a pressing block, the pressing block is detachably connected with the main body, the pressing block is provided with a liquid adding flow channel extending in the first direction, and the liquid adding flow channel is communicated with the liquid injection hole of the flow cell; one end of the collecting pipe is inserted into the bottom of the main body and is communicated with the liquid discharge hole of the flow cell; the collecting container is arranged on the bearing frame, and the other end of the collecting pipe is located in the collecting container. According to the utility model, samples or reagents can be manually added into the flow cell, the consumption of electric energy is reduced, and the occupied space is small.

Description

Technical Field

[0001] The utility model relates to the technical field of gene sequencing, in particular to a manual loading instrument. Background Art

[0002] Among current medical testing technologies, next-generation sequencing (NGS) is a test that detects the base sequence of deoxyribonucleic acid at the molecular scale. It facilitates the precise location and detection of difficult clinical conditions and epidemic diseases. Its main principle is to use a flow cell as a carrier for biochemical reactions, then detect the feedback signal from the flow cell through optical or electronic means to determine the type of base currently being detected.

[0003] In the existing technology, the flow cell can only be placed inside the sequencing device, and the liquid adding mechanism inside the sequencing device is used to automatically add samples or reagents through an electric pump. However, the overall area occupied by the sequencing device is large and requires power support.

[0004] Therefore, it is urgent to provide a manual loading instrument to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide a manual loading instrument, which can not only add samples or reagents to a flow cell in a manual manner to reduce power consumption, but also takes up little space.

[0006] To achieve the above objectives, the following technical solutions are provided:

[0007] Manual loader, including:

[0008] A bracket, wherein the bracket is provided with a card slot and a carrying frame at intervals along a first direction;

[0009] A main body, inserted into the card slot, the main body is provided with a receiving groove extending along a first direction, and the flow cell can be placed in the receiving groove;

[0010] a liquid adding assembly, comprising a pressing block, the pressing block being detachably connected to the main body, the pressing block being provided with a liquid adding channel extending along a first direction, the liquid adding channel being connected to the liquid injection hole of the flow cell;

[0011] A manifold, one end of which is plugged into the bottom of the main body and communicated with the drainage hole of the flow pool;

[0012] A collecting container is arranged on the carrying frame, and the other end of the collecting pipe is located in the collecting container.

[0013] As an optional solution for the manual loading instrument, the main body is provided with a clamping portion on two opposite sides, and the liquid adding assembly further includes:

[0014] A buckle, the buckle being rotatably connected to the pressing block;

[0015] An elastic return member is provided between one end of the buckle and the pressing block, and the other end of the buckle can be engaged with the engaging portion.

[0016] As an optional solution for the manual loading instrument, a positioning guide hole is provided at the bottom of the pressing block, and a positioning guide column is provided at the upper part of the main body, and the positioning guide column is plugged into the positioning guide hole.

[0017] As an optional solution for the manual loading instrument, the pressure block is provided with a limit plate at one end away from the positioning guide hole, the limit plate is provided with a guide part, the main body is provided with a guide groove, and the guide part can be plugged into the guide groove.

[0018] As an optional solution for the manual loading instrument, the pressing block is provided with a light-transmitting hole extending along the second direction, and the light-transmitting hole is communicated with the liquid-adding channel.

[0019] As an optional solution for the manual loading instrument, the main body is provided with a pick-up and placement avoidance groove extending along the third direction, and the pick-up and placement avoidance groove is connected to the side wall of the accommodating groove.

[0020] As an optional solution of the manual loader, the manual loader also includes:

[0021] a first sealing gasket, embedded in the first mounting groove of the pressure block, the first sealing gasket being provided with a liquid adding channel extending along a first direction, the liquid adding flow channel, the liquid adding channel and the liquid injection hole of the flow pool being connected in sequence;

[0022] The second sealing gasket is embedded in the second installation groove of the main body. The second sealing gasket is provided with a drainage channel extending along the first direction. The drainage hole of the flow pool, the drainage channel and the collecting pipe are connected in sequence.

[0023] As an optional solution of the manual loading instrument, the end surface of the main body is provided with a first connecting hole, and the manual loading instrument further comprises:

[0024] A pressing plate is provided with a second connecting hole, and a fastener passes through the second connecting hole and is connected to the first connecting hole, so that the second sealing gasket is clamped between the pressing plate and the main body.

[0025] As an optional solution for the manual loader, a rib is provided circumferentially at the opening of the collection container, a through hole is provided on the carrying frame, the collection container is inserted into the through hole and the rib abuts against the carrying frame.

[0026] As an alternative to the manual loader, the side wall of the carrying frame is provided with an observation window.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The manual loading instrument provided by the present invention installs the main body into the card slot located above the bracket, the collection container is installed on the supporting frame located below the bracket, and the flow pool is installed into the receiving slot of the main body. The liquid adding channel on the pressing block is correspondingly connected to the liquid injection hole located above the flow pool, one end of the collecting pipe is correspondingly connected to the liquid discharge hole located below the flow pool, and the other end of the collecting pipe is inserted into the collection container. After the operator manually adds the sample or reagent to the liquid adding channel, the liquid can be loaded into the flow pool by its own gravity without the need for additional external force, thus reducing the consumption of electrical energy. The excess liquid flows back to the collection container through the collecting pipe, thus avoiding leakage of excess liquid. The components of the manual loading instrument are all arranged along the first direction, thus reducing the occupied space. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0030] Figure 1 This is a schematic assembly diagram of the manual loader in the embodiment of the present utility model from a first perspective;

[0031] Figure 2 This is a schematic diagram of the assembly of the manual loader from a second viewing angle in an embodiment of the present utility model;

[0032] Figure 3 This is an exploded schematic diagram of a manual loading instrument in an embodiment of the present utility model;

[0033] Figure 4 This is an exploded schematic diagram of the liquid adding assembly in the embodiment of the present utility model;

[0034] Figure 5 This is a schematic structural diagram of the pressing block in an embodiment of the present utility model.

[0035] Reference numerals:

[0036] 100, flow cell; 101, injection hole;

[0037] 1. Bracket; 2. Main body; 3. Liquid adding assembly; 4. Manifold; 5. Collection container; 51. Rib; 6. First sealing gasket; 61. Liquid adding channel; 7. Second sealing gasket; 71. Liquid drain channel; 8. Pressing piece; 81. Second connecting hole; 9. Adapter;

[0038] 11. Card slot; 12. Carrying frame; 121. Through hole; 122. Observation window;

[0039] 21. Accommodation groove; 22. First connecting hole; 23. Clamping portion; 24. Positioning guide column; 25. Guide groove; 26. Pick-up and drop avoidance groove; 27. Second installation groove;

[0040] 31. Pressing block; 311. Liquid adding channel; 312. Positioning guide hole; 313. Limiting plate; 3131. Guide portion; 314. Light-transmitting hole; 315. First mounting slot; 32. Buckle; 33. Elastic reset member; 34. Rotating shaft pin. DETAILED DESCRIPTION

[0041] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0042] In the description of the present utility model, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, or are the orientation or position relationship in which the utility model product is usually placed when in use. They are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0043] It should also be noted that, in the description of this utility model, unless otherwise specified or limited, the terms "disposed" and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections. Those skilled in the art will understand the specific meanings of these terms in this utility model based on the specific circumstances.

[0044] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0045] In order to manually add samples or reagents to the flow cell, reduce power consumption, and take up less space, this embodiment provides a manual loading instrument. Figures 1 to 5 The specific contents of this embodiment are described in detail. It should be noted that the first direction mentioned in this embodiment is Figure 1 The Z direction in this embodiment is the second direction Figure 1 The Y direction in this embodiment is the third direction Figure 1 The X direction in .

[0046] like Figures 1 to 4 As shown, the manual loader in this embodiment includes a bracket 1, a main body 2, a liquid adding assembly 3, a collecting pipe 4 and a collecting container 5. The bracket 1 is provided with a card slot 11 and a supporting frame 12 at intervals along the first direction. The main body 2 is inserted into the card slot 11, and the main body 2 is provided with a receiving groove 21 extending along the first direction, and the flow pool 100 can be placed in the receiving groove 21. The liquid adding assembly 3 includes a pressure block 31, which is detachably connected to the main body 2, and is provided with a liquid adding channel 311 extending along the first direction on the pressure block 31, and the liquid adding channel 311 is connected to the liquid injection hole 101 of the flow pool 100. The collecting container 5 is provided on the supporting frame 12. One end of the collecting pipe 4 is inserted into the bottom of the main body 2 through the adapter 9 and is connected to the drainage hole of the flow pool 100; the other end of the collecting pipe 4 is located in the collecting container 5.

[0047] Further, if Figure 1 、 Figure 3 and Figure 5 As shown, the manual loader also includes a first sealing gasket 6 and a second sealing gasket 7. The first sealing gasket 6 is embedded in the first mounting groove 315 of the pressure block 31, and the first sealing gasket 6 is provided with a liquid adding channel 61 extending along the first direction. The liquid adding channel 311, the liquid adding channel 61 and the liquid injection hole 101 of the flow pool 100 are connected in sequence. The second sealing gasket 7 is embedded in the second mounting groove 27 of the main body 2, and the second sealing gasket 7 is provided with a liquid discharge channel 71 extending along the first direction. The liquid discharge hole of the flow pool 100, the liquid discharge channel 71 and the manifold 4 are connected in sequence. By adding the first sealing gasket 6 and the second sealing gasket 7, the sealing of the flow channel is ensured to prevent liquid seepage and the infiltration of external contaminants.

[0048] The manual loading instrument provided in this embodiment installs the main body 2 into the card slot 11 located above the bracket 1, the collection container 5 is installed on the supporting frame 12 located below the bracket 1, and the flow cell 100 is installed into the receiving groove 21 of the main body 2. The liquid adding channel 311 on the pressing block 31, the liquid adding channel 61 of the first sealing gasket 6, the injection hole 101 located above the flow cell 100, and the drainage channel 71 of the second sealing gasket 7 are correspondingly connected to form a complete flow channel. One end of the manifold 4 is correspondingly connected to the drainage channel 71 of the second sealing gasket 7 located below the flow cell 100, and the other end of the manifold 4 is inserted into the collection container 5. When the operator manually adds the sample or reagent to the liquid adding channel 311, the liquid can be loaded into the flow cell 100 by its own gravity without the need for additional external force, reducing the consumption of electrical energy. The excess liquid flows back to the collection container 5 through the manifold 4 for recycling the excess liquid. It is simple, convenient, low-cost, and easy to operate. The components of this manual loading instrument are all arranged along the first direction, which reduces the occupied space.

[0049] Further, if Figures 3 to 5 As shown, the main body 2 is provided with a clamping portion 23 on opposite sides, and the liquid adding assembly 3 further includes a buckle 32 and an elastic reset member 33. Both buckles 32 are rotatably connected to opposite sides of the pressure block 31 via a rotating shaft pin 34. The elastic reset member 33 is disposed between one end of the buckle 32 and the pressure block 31, and the other end of the buckle 32 can be engaged with the clamping portion 23. In this embodiment, the elastic reset member 33 is a helical compression spring, and the operator can disengage the buckle 32 from the clamping portion 23 by pressing one end of the buckle 32.

[0050] Furthermore, a positioning guide hole 312 is provided at the bottom of the pressing block 31, and a positioning guide post 24 is provided at the upper portion of the main body 2, which is plugged into the positioning guide hole 312. By adding the positioning guide hole 312 and the positioning guide post 24, it is easy to guide the pressing block 31 to quickly and accurately match with the main body 2.

[0051] Furthermore, two stop plates 313 are provided at one end of the pressing block 31, away from the positioning guide hole 312. Each stop plate 313 has a guide portion 3131. The main body 2 is provided with a guide groove 25, and the guide portions 3131 can be inserted into the guide groove 25. During the mating process of the pressing block 31 and the main body 2, the guide portions 3131 move along the side walls of the guide groove 25 to complete the assembly. The guide portions 3131 also block the upper half of the flow cell 100, preventing the flow cell 100 from falling off.

[0052] Further, if Figure 1 Combine Figure 3As shown, the pressing block 31 is provided with a light-transmitting hole 314 extending along the second direction, and the light-transmitting hole 314 is connected to the liquid-adding channel 311. Since the first direction is perpendicular to the second direction, the light-transmitting hole 314 is vertically connected to the liquid-adding channel 311. By adding the light-transmitting hole 314, on the one hand, it serves to transmit light to the liquid-adding channel 311, and on the other hand, it is convenient for the operator to observe whether the pipette tip is properly placed.

[0053] Furthermore, the main body 2 is provided with a pick-and-place clearance groove 26 extending along the third direction. The pick-and-place clearance groove 26 penetrates the sidewall of the accommodating tank 21. In this embodiment, the flow cell 100 is placed along the first direction. Since the first direction is perpendicular to the third direction, when the flow cell 100 needs to be removed from or placed in the main body 2, the pick-and-place clearance groove 26 can be used to grasp the flow cell 100, facilitating the removal and placement of the flow cell 100. This simplifies the operation of loading the flow cell 100, allowing the flow cell 100 to be placed in the corresponding position and locked with a single hand.

[0054] Optionally, the depths of the pick-and-place avoidance groove 26 , the accommodating groove 21 and the guide groove 25 decrease in sequence.

[0055] Further, if Figure 3 As shown, the end face of the main body 2 is provided with a first connecting hole 22, and the manual loader also includes a pressing piece 8, which is provided with a second connecting hole 81. The fastener passes through the second connecting hole 81 and is connected to the first connecting hole 22, so that the second sealing gasket 7 is clamped between the pressing piece 8 and the main body 2. By adding the pressing piece 8, the installation stability of the second seal is improved and the second seal is prevented from falling off. The horizontally designed clamping method avoids the risk of the flow cell 100 being bounced upward, the interface of the flow cell 100 being poorly sealed, or the flow cell 100 being greatly deformed.

[0056] Further, if Figure 3 As shown, the opening of the collection container 5 is provided with a circumferential retaining edge 51, and the carrier frame 12 is provided with a through hole 121. The collection container 5 is inserted into the through hole 121, and the retaining edge 51 abuts against the carrier frame 12. The carrier frame 12 is hollow inside. By adding the through hole 121, the collection container 5 can be inserted into the carrier frame 12, and by adding the retaining edge 51, the collection container 5 can be hung on the carrier frame 12.

[0057] Further, if Figure 2 As shown, the side wall of the carrying frame 12 is provided with an observation window 122. Specifically, the observation window 122 is located at the back of the carrying frame 12, which is convenient for the operator to observe the capacity of the liquid inside the collection container 5.

[0058] Note that the above are merely preferred embodiments of the present invention and the technical principles employed. Those skilled in the art will appreciate that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, and substitutions are possible for those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments and may include many other equivalent embodiments without departing from the scope of the present invention. The scope of the present invention is determined by the scope of the appended claims.

Claims

1. Manual loader, characterized in that, include: A bracket (1), wherein the bracket (1) is provided with a card slot (11) and a carrying frame (12) spaced apart along a first direction; A main body (2) is inserted into the card slot (11), and the main body (2) is provided with a receiving groove (21) extending along a first direction, and the flow cell (100) can be placed in the receiving groove (21); A liquid adding assembly (3) comprising a pressing block (31), the pressing block (31) being detachably connected to the main body (2), the pressing block (31) being provided with a liquid adding channel (311) extending along a first direction, the liquid adding channel (311) being in communication with the liquid injection hole (101) of the flow cell (100); A manifold (4), one end of which is plugged into the bottom of the main body (2) and communicates with the drainage hole of the flow pool (100); A collecting container (5) is arranged on the carrying frame (12), and the other end of the collecting pipe (4) is located in the collecting container (5).

2. The manual loading instrument according to claim 1, characterized in that: The main body (2) is provided with a clamping portion (23) on two opposite sides thereof, and the liquid adding assembly (3) further comprises: A buckle (32), the buckle (32) being rotatably connected to the pressing block (31); An elastic reset member (33) is provided between one end of the buckle (32) and the pressing block (31), and the other end of the buckle (32) can be engaged with the engaging portion (23).

3. The manual loading instrument according to claim 1, characterized in that: A positioning guide hole (312) is provided at the bottom of the pressing block (31), and a positioning guide column (24) is provided at the upper portion of the main body (2), and the positioning guide column (24) is plugged into the positioning guide hole (312).

4. The manual loading instrument according to claim 3, characterized in that: The pressure block (31) is provided with a limiting plate (313) at one end away from the positioning guide hole (312), and the limiting plate (313) is provided with a guide portion (3131). The main body (2) is provided with a guide groove (25), and the guide portion (3131) can be plugged into the guide groove (25).

5. The manual loading instrument according to claim 1, characterized in that: The pressing block (31) is provided with a light-transmitting hole (314) extending along the second direction, and the light-transmitting hole (314) is communicated with the liquid-adding channel (311).

6. The manual loading instrument according to claim 1, characterized in that: The main body (2) is provided with a pick-up and placement avoidance groove (26) extending along a third direction, and the pick-up and placement avoidance groove (26) is connected to the side wall of the accommodating groove (21).

7. The manual loading instrument according to claim 1, characterized in that: The manual loading instrument also includes: a first sealing gasket (6) embedded in a first mounting groove (315) of the pressure block (31), the first sealing gasket (6) being provided with a liquid adding channel (61) extending along a first direction, the liquid adding channel (311), the liquid adding channel (61) and the liquid injection hole (101) of the flow cell (100) being sequentially connected; A second sealing gasket (7) is embedded in a second mounting groove (27) of the main body (2). The second sealing gasket (7) is provided with a drainage channel (71) extending along a first direction. The drainage hole of the flow pool (100), the drainage channel (71) and the manifold (4) are sequentially connected.

8. The manual loading instrument according to claim 7, characterized in that: The end surface of the main body (2) is provided with a first connecting hole (22), and the manual loading instrument further comprises: A pressing plate (8), wherein the pressing plate (8) is provided with a second connecting hole (81), and a fastener passes through the second connecting hole (81) and is connected to the first connecting hole (22), so that the second sealing gasket (7) is clamped between the pressing plate (8) and the main body (2).

9. The manual loading instrument according to claim 1, characterized in that: A retaining edge (51) is provided circumferentially at the opening of the collection container (5); a through hole (121) is provided on the carrying frame (12); the collection container (5) is inserted into the through hole (121) and the retaining edge (51) abuts against the carrying frame (12).

10. The manual loading instrument according to any one of claims 1 to 9, characterized in that: An observation window (122) is provided on the side wall of the carrying frame (12).

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