Test paper assembly for detecting tetracyclines in dairy products

By designing the test strip assembly for sealing membrane components and sample loading components, the inaccurate detection results caused by the long-term contact between the colloidal gold reagent card and the outside world, achieving high accuracy of tetracycline detection in dairy products.

CN223205489UActive Publication Date: 2025-08-08XINUOTONGKE (TIANJIN) BIOTECHNOLOGY CO LTD +1
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Patent Information

Application Number
CN202421504706.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-28
Publication Date
2025-08-08
Estimated Expiration
2034-06-28

AI Technical Summary

Technical Problem

The existing colloidal gold reagents are stuck in the tetracycline test of dairy products. Long-term contact with the external environment will lead to the entry of pollutants and changes in the reagent concentration, affecting the accuracy of the detection results.

Method used

A test paper assembly including a sealing membrane assembly, an observation assembly and a sample loading assembly is designed. The sample slot and display slot are sealed by the sealing membrane assembly, and the combination of the sample loading assembly and the observation assembly are combined to ensure that the detection process is carried out in a completely sealed state.

Benefits of technology

It effectively avoids the entry of pollutants and changes in reagent concentration, improves the accuracy of detection results, and reduces the occurrence of false positive or false negative results.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides a test paper component for detecting tetracyclines in dairy products, which comprises a sealing membrane component, and the sealing membrane component is heat-sealed on the surface of a tetracycline detection reagent card and is used for sealing colloidal gold test paper exposed at a sample groove and a display groove; the sample adding assembly comprises a shell, the shell is slidably connected to the outer side wall of the tetracycline detection reagent card and clamped with the tetracycline detection reagent card, an observation groove is formed in the top of the shell, and a sealing assembly is movably connected into the observation groove; the sample adding assembly is used for being matched with the observation assembly to dropwise add a sample into the sample groove of the tetracycline detection reagent card. According to the tetracycline detection reagent card detection device, the sealing film assembly, the sample adding assembly and the observation assembly are additionally arranged, so that the tetracycline detection reagent card can be detected in a state of being isolated from the outside after the packaging bag is taken out; and the accuracy of a detection result is ensured to the greatest extent.
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Description

Technical Field

[0001] The utility model relates to the field of dairy product detection, in particular to a test paper assembly for detecting tetracyclines in dairy products. Background Art

[0002] As an important part of people's daily diet, the safety of dairy products has attracted much attention. Tetracycline, as a broad-spectrum antibiotic, is widely used in animal husbandry, but its residue problem in dairy products has also attracted people's attention. Tetracycline residues may not only have a negative impact on human health, but also affect the quality and taste of dairy products. Therefore, it is particularly important to detect tetracycline in dairy products; currently, the main methods for detecting tetracycline in dairy products are high performance liquid chromatography, microbiological methods, etc.; these methods have their own advantages and disadvantages, but all have problems such as complex operation and long time consumption; in recent years, with the continuous development of science and technology, new technologies such as ultra-high performance liquid chromatography and liquid chromatography-mass spectrometry have gradually been applied to tetracycline detection, which has improved the accuracy and sensitivity of detection;

[0003] The colloidal gold reagent card is an immunoassay reagent card based on colloidal gold technology and can be used for the rapid detection of tetracycline in dairy products. Colloidal gold technology is characterized by its ease of operation, rapidity, and sensitivity, and has broad application prospects in the field of biochemical immunoassays. The colloidal gold reagent card mainly consists of a test strip, a sample injection port, and a sealing plug. The test strip is coated with specific antibodies and colloidal gold markers, which can specifically identify tetracycline residues in dairy products. During the test process, a dairy sample is added to the sample injection port, where the sample reacts specifically with the antibodies and colloidal gold markers on the test strip, forming a visible color reaction, thereby achieving rapid detection of tetracycline residues.

[0004] Although existing colloidal gold reagent cards have certain advantages in tetracycline detection in dairy products, long-term contact with the external environment during use may lead to defects that affect the test results; first, the sample injection port of the reagent card is open, which makes it easy for pollutants such as dust and microorganisms in the air to enter. These pollutants may interfere with the immune response on the reagent card, resulting in false positive or false negative results; second, some reagents in the colloidal gold reagent card may be volatile, and long-term exposure to air may cause changes in reagent concentration, thereby affecting the accuracy of the test results. Utility Model Content

[0005] In order to solve the problem that the tetracycline detection reagent card in the prior art is in contact with the external environment for a long time during use, which may affect the test results, the utility model provides a test paper assembly for the detection of tetracyclines in dairy products;

[0006] The utility model provides a test strip assembly for detecting tetracyclines in dairy products using the following technical solutions:

[0007] A test strip assembly for detecting tetracyclines in dairy products, comprising a tetracycline detection reagent card, wherein the tetracycline detection reagent card includes a sample slot and a display slot; and further comprising:

[0008] A sealing film assembly, which is heat-sealed on the surface of the tetracycline detection reagent card and is used to seal the exposed colloidal gold test paper at the sample slot and the display slot;

[0009] The observation component includes a housing, the housing is slidably connected to the outer wall of the tetracycline detection reagent card and is engaged with the tetracycline detection reagent card. The top of the housing is provided with an observation groove, and the interior of the observation groove is movably connected to a sealing component;

[0010] A sample adding component, which is used to cooperate with the observation component to add samples to the sample slot of the tetracycline detection reagent card;

[0011] Furthermore, the sealing film assembly includes a laminating film, a pull strip, and an extension sheet; the surface of the tetracycline detection reagent card is heat-sealed with a laminating mold, one end of the laminating mold is integrally formed with a pull strip, the pull strip is folded inversely along the end connected to the laminating film, and is integrally formed with an extension sheet;

[0012] Furthermore, a card block is integrally formed at the bottom of the tetracycline detection reagent card, and a card slot is provided at the bottom of the shell; the card block and the card slot can be engaged and fixed with each other;

[0013] Furthermore, the sealing assembly includes a pressure plate, an observation cover, an elastic member, a sample cover, a sealing ring, and an injection port. The observation cover is provided in the observation tank; the bottom of the observation cover is integrally formed with a pressure plate; the elastic member is welded between the pressure plate and the inner side wall of the shell; the inner side wall of the observation cover is integrally formed with a sample cover for covering the sample tank; the bottom of the pressure plate is integrally formed with a sealing ring that covers the sample tank and the display tank; the top of the observation tank is provided with an injection port; the injection port is connected to the interior of the sample cover;

[0014] Furthermore, the elastic member is one of a spring and a metal reed; the number of the elastic members is N, where N≥2;

[0015] Furthermore, a rubber stopper is provided at the injection port;

[0016] Furthermore, the pressing plate, observation cover, sample cover, and sealing ring are all made of transparent acrylic material;

[0017] Furthermore, the sample addition assembly includes a sample tube, a liquid discharge port, a piston rod, a push plate, and a baffle; the sample tube is movably connected to the piston rod; the piston rod is provided at one end disposed inside the sample tube, and the push rod is integrally formed at one end disposed outside the sample tube; the outer wall of the sample tube is provided with a liquid discharge port that matches the injection port; and the baffle is integrally formed on the outer wall of the sample tube;

[0018] Furthermore, the outer side wall of the sample tube is covered with an anti-slip sleeve; the anti-slip sleeve is made of rubber;

[0019] Furthermore, the tetracycline detection reagent card, observation component, and sample addition component are placed in the same packaging bag and combined.

[0020] In summary, the beneficial effects of the present invention are as follows:

[0021] The utility model achieves the goal of enabling the tetracycline detection reagent card to be isolated from the outside world after the packaging bag is taken out by adding a sealing film component, a sample adding component, and an observation component, thereby ensuring the accuracy of the test result to the greatest extent. When in use, the tetracycline detection reagent card is inserted into the interior of the shell, and then the extension piece is pulled by hand to remove the covering film from the surface of the tetracycline detection reagent card. At the same time, the pressing plate tightly adheres the sealing ring to the display slot and the sample slot under the action of the elastic member, and forms a completely closed space isolated from the outside world by the surface of the tetracycline detection reagent card; then the sample cover can cover the sample slot alone, and then the user can add the sample to the sample tube, and then insert the piston rod to make the discharge port discharge air upward, and then insert the discharge port into the injection port, push the push plate, and the piston rod can inject the sample into the sample slot in the sample cover, and wait for a period of time to observe the result displayed on the display slot through the observation cover;

[0022] The utility model avoids prolonged contact with the external environment during use through the above-mentioned design; it also prevents the entry of pollutants such as dust and microorganisms in the air, reducing the occurrence of false positive or false negative results; and further prevents certain reagents in the colloidal gold reagent card from being exposed to the air for a long time, which may cause changes in the reagent concentration, thereby improving the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0024] Figure 2 This is a schematic diagram of the overall structure of the tetracycline detection reagent card in the present invention;

[0025] Figure 3 This is a schematic diagram of the overall structure of the sealing membrane assembly of the utility model;

[0026] Figure 4 This is a schematic diagram of the overall structure of the sample adding component of the utility model;

[0027] Figure 5 This is a schematic diagram of the overall structure of the housing of the utility model;

[0028] Figure 6 This is a schematic diagram of the back of the tetracycline detection reagent card of the present invention;

[0029] Figure 7 This is a schematic diagram of the overall structure of the observation cover of the utility model;

[0030] Figure 8 This is a schematic diagram of the back structure of the observation cover of the present utility model.

[0031] As shown in the figure: 1-Tetracycline detection reagent card, 11-sample slot, 12-display slot, 2-slide slot, 3-laminated film, 31-pull strip, 32-extension sheet, 4-housing, 41-card slot, 42-slider, 43-observation slot, 44-block, 5-pressing plate, 51-observation cover, 52-elastic part, 53-sample cover, 54-sealing ring, 55-injection port, 6-sample tube, 61-drain port, 62-piston rod, 63-push plate, 64-baffle, 65-anti-slip sleeve. DETAILED DESCRIPTION

[0032] The following is combined with Figure 1-8 The utility model is further described in detail:

[0033] The present invention discloses a test strip assembly for detecting tetracyclines in dairy products. Figure 1 、 Figure 2 、 Figure 4 As shown, a test strip assembly for detecting tetracyclines in dairy products includes a tetracycline detection reagent card 1, wherein the tetracycline detection reagent card 1 includes a sample slot 11 and a display slot 12; and further includes:

[0034] A sealing film assembly is heat-sealed on the surface of the tetracycline detection reagent card 1 to seal the exposed colloidal gold test paper at the sample slot 11 and the display slot 12;

[0035] The observation component and the sample adding component include a housing 4, which is slidably connected to the outer wall of the tetracycline detection reagent card 1 and is engaged with the tetracycline detection reagent card 1. The top of the housing 4 is provided with an observation groove 43, and the interior of the observation groove 43 is movably connected to a sealing component;

[0036] The sample adding component is used to cooperate with the observation component to add the sample to the sample slot 11 of the tetracycline detection reagent card 1; in this embodiment, the sample slot 11 and the display slot 12 of the tetracycline detection reagent card 1 are sealed by the sealing film component, so that when the tetracycline detection reagent card 1 is taken out of the packaging bag, the colloidal gold test paper inside is prevented from being contaminated by the outside world and affecting the detection effect. In addition, the combination of the sample adding component and the observation component can enable the tetracycline detection reagent card 1 to complete the tetracycline detection in a completely sealed state without contact with the outside world, thereby ensuring the accuracy of the test results to the greatest extent; this embodiment is easy to use, and only the tetracycline detection reagent card 1 needs to be inserted into the housing 4, the sealing film component is pulled open, and the sample is added to the sample slot 11 using the observation component;

[0037] like Figure 2 、 Figure 3 As shown, the sealing film assembly includes a coating film 3, a pull strip 31, and an extension piece 32; the surface of the tetracycline detection reagent card 1 is heat-sealed with a coating mold, and one end of the coating mold is integrally formed with a pull strip 31, and the pull strip 31 is folded inversely along the end connected to the coating film 3 and integrally formed with an extension piece 32; in this embodiment, after the tetracycline detection reagent card 1 is inserted into the housing 4 and stuck, the extension piece 32 is pulled, and the coating film 3 can be removed from the surface of the tetracycline detection reagent card 1 through the pull strip 31, and then the display slot 12 and the sample slot 11 are covered and sealed again by the sealing assembly to prevent contact with the external environment;

[0038] like Figure 5 、 6 As shown, a card block 44 is integrally formed at the bottom of the tetracycline detection reagent card 1, and a card slot 41 is provided at the bottom of the housing 4; the card block 44 and the card slot 41 can be engaged and fixed with each other; in this embodiment, the card block 44 and the card slot 41 are both circular key-type structures, and the outer wall of the card block 44 is chamfered to facilitate the card block 44 to be smoothly engaged in the card slot 41 for sealing;

[0039] like Figure 7 、 8As shown, the sealing assembly includes a pressing plate 5, an observation cover 51, an elastic member 52, a sample cover 53, a sealing ring 54, and an injection port 55. The observation cover 51 is provided in the observation groove 43; the bottom of the observation cover 51 is integrally formed with a pressing plate 5; an elastic member 52 is welded between the pressing plate 5 and the inner side wall of the shell 4; the inner side wall of the observation cover 51 is integrally formed with a sample cover 53 for covering the sample groove 11; the bottom of the pressing plate 5 is integrally formed with a sealing ring 54 that covers the sample groove 11 and the display groove 12; an injection port 55 is provided on the top of the observation lens ... The inlet 55 is connected to the interior of the sample cover 53; the pressing plate 5, the observation cover 51, the sample cover 53, and the sealing ring 54 are all made of transparent acrylic material; in this embodiment, when the laminating film 3 is removed, the pressing plate 5, under the action of the elastic member 52, tightly adheres the sealing ring 54 to the periphery of the display slot 12 and the sample slot 11, and forms a completely enclosed space isolated from the outside world by the surface of the tetracycline detection reagent card 1; thereafter, the sample cover 53 can cover the sample slot 11 alone to prevent excessive sample from flowing into the display slot 12 and affecting the test results;

[0040] In this embodiment, the pressing plate 5, the observation cover 51, the sample cover 53, and the sealing ring 54 are all made of transparent acrylic material to facilitate the user to further observe the test results;

[0041] like Figure 7 As shown, the elastic member 52 is one of a spring and a metal reed; the number of the elastic members 52 is N, where N≥2; in this embodiment, both the spring and the metal reed can be used, and their purpose and function are to help the sealing ring 54 fit more tightly with the tetracycline detection reagent card 1;

[0042] A rubber stopper is provided at the injection port 55; in this embodiment, the user needs to remove the rubber stopper before injecting the sample into the injection port 55, and the use of the rubber stopper can also avoid contact with the outside air;

[0043] like Figure 4 As shown, the sample adding assembly includes a sample tube 6, a liquid discharge port 61, a piston rod 62, a push plate 63, and a baffle 64; the interior of the sample tube 6 is movably connected with a piston rod 62; one end of the piston rod 62 is provided with a piston inside the sample tube 6, and the end of the sample tube 6 is provided on the outside of the sample tube 6 and is integrally formed with a push rod; the outer wall of the sample tube 6 is provided with a liquid discharge port 61 that matches the injection port 55; the outer wall of the sample tube 6 is integrally formed with a baffle 64; in this embodiment, the user can first add the sample to the sample tube 6, and then insert the piston rod 62 to allow the liquid discharge port 61 to discharge air upward, and then insert the liquid discharge port 61 into the injection port 55, push the push plate 63, and the piston rod 62 can inject the sample into the sample slot 11 in the sample cover 53, and wait for a while before observing the results displayed on the display slot 12 through the observation cover 51;

[0044] like Figure 4 As shown, the outer wall of the sample tube 6 is covered with an anti-slip cover 65; the anti-slip cover 65 is made of rubber; in this embodiment, the anti-slip cover 65 can better enable the user to hold the sample tube 6 to avoid slipping;

[0045] The tetracycline detection reagent card 1, the observation component, and the sample adding component are placed in the same packaging bag and combined. In this embodiment, the tetracycline detection reagent card 1, the observation component, and the sample adding component are a complete set of detection components, which can be packaged separately for use and are all disposable products to avoid reuse.

[0046] The implementation principle of the embodiment of the present utility model is:

[0047] When using the present invention, the user needs to open the packaging bag, take out the present invention in its entirety, insert the tetracycline detection reagent card 1 into the interior of the shell 4, then pull the extension piece 32 by hand to remove the coating film 3 from the surface of the tetracycline detection reagent card 1. At the same time, the pressing plate 5, under the action of the elastic member 52, tightly adheres the sealing ring 54 to the display slot 12 and the sample slot 11, and forms a completely enclosed space isolated from the outside world by the surface of the tetracycline detection reagent card 1; then the sample cover 53 can cover the sample slot 11 alone, and then the user can add the sample to the sample tube 6, and then insert the piston rod 62 to make the drain port 61 discharge air upward, and then insert the drain port 61 into the injection port 55, push the push plate 63, and the piston rod 62 can inject the sample into the sample slot 11 in the sample cover 53. After waiting for a while, the result displayed on the display slot 12 can be observed through the observation cover 51.

[0048] The above shows and describes the basic principles, main features and advantages of the present invention. The various components mentioned in the present invention are common technologies in the existing field. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements fall within the scope of the present invention. The scope of protection claimed in this utility model is defined by the appended claims and their equivalents.

Claims

1. A test strip assembly for detecting tetracyclines in dairy products, comprising a tetracycline detection reagent card (1), wherein the tetracycline detection reagent card (1) comprises a sample slot (11) and a display slot (12); characterized in that: Also includes: A sealing film assembly, wherein the sealing film assembly is heat-sealed on the surface of the tetracycline detection reagent card (1) and is used to seal the exposed colloidal gold test paper at the sample slot (11) and the display slot (12); An observation component, comprising a housing (4), the housing (4) being slidably connected to the outer wall of the tetracycline detection reagent card (1) and engaging with the tetracycline detection reagent card (1), an observation groove (43) being provided on the top of the housing (4), and a sealing component being movably connected inside the observation groove (43); The sample adding component is used to cooperate with the observation component to drop the sample into the sample slot (11) of the tetracycline detection reagent card (1).

2. A test strip assembly for detecting tetracyclines in dairy products according to claim 1, characterized in that The sealing film assembly comprises a laminating film (3), a pull strip (31), and an extension piece (32); the surface of the tetracycline detection reagent card (1) is heat-sealed with a laminating mold, one end of the laminating mold is integrally formed with a pull strip (31), and the pull strip (31) is folded in reverse along the end connected to the laminating film (3) and is integrally formed with an extension piece (32).

3. A test strip assembly for detecting tetracyclines in dairy products according to claim 1, characterized in that The bottom of the tetracycline detection reagent card (1) is integrally formed with a card block (44), and the bottom of the shell (4) is provided with a card slot (41); the card block (44) and the card slot (41) can be mutually engaged and fixed.

4. A test strip assembly for detecting tetracyclines in dairy products according to claim 1, characterized in that The sealing assembly comprises a pressing plate (5), an observation cover (51), an elastic member (52), a sample cover (53), a sealing ring (54), and an injection port (55); the observation cover (51) is provided in the observation slot (43); the bottom of the observation cover (51) is integrally formed with a pressing plate (5); an elastic member (52) is welded between the pressing plate (5) and the inner side wall of the housing (4); the inner side wall of the observation cover (51) is integrally formed with a sample cover (53) for covering the sample slot (11); the bottom of the pressing plate (5) is integrally formed with a sealing ring (54) for covering the sample slot (11) and the display slot (12); an injection port (55) is provided at the top of the observation cover (51); and the injection port (55) is communicated with the interior of the sample cover (53).

5. A test strip assembly for detecting tetracyclines in dairy products according to claim 4, characterized in that The elastic member (52) is one of a spring and a metal spring leaf; the number of the elastic members (52) is N, where N≥2.

6. A test strip assembly for detecting tetracyclines in dairy products according to claim 4, characterized in that The injection port (55) is provided with a rubber stopper.

7. A test strip assembly for detecting tetracyclines in dairy products according to claim 4, characterized in that The pressing plate (5), the observation cover (51), the sample cover (53), and the sealing ring (54) are all made of transparent acrylic material.

8. A test strip assembly for detecting tetracyclines in dairy products according to claim 4, characterized in that The sample addition assembly comprises a sample tube (6), a liquid discharge port (61), a piston rod (62), a push plate (63), and a baffle (64); the interior of the sample tube (6) is movably connected to the piston rod (62); one end of the piston rod (62) disposed inside the sample tube (6) is provided with a piston, and one end of the sample tube (6) disposed outside the sample tube (6) is integrally formed with a push rod; the outer wall of the sample tube (6) is provided with a liquid discharge port (61) that matches the injection port (55); and the outer wall of the sample tube (6) is integrally formed with a baffle (64).

9. A test strip assembly for detecting tetracyclines in dairy products according to claim 8, characterized in that The outer side wall of the sample tube (6) is sheathed with an anti-slip sleeve (65); the anti-slip sleeve (65) is made of rubber.

10. A test strip assembly for detecting tetracyclines in dairy products according to claim 1, characterized in that The tetracycline detection reagent card (1), the observation component, and the sample adding component are placed in the same packaging bag and combined.