Microscopic imaging device
Through the integrated microscopic imaging equipment with quantitative phase imaging, fluorescence imaging and bright field imaging functions, the problem that existing equipment can only have a single imaging mode is solved, and convenient switching of multiple imaging modes is achieved, improving efficiency and reducing costs.
Patent Information
- Application Number
- CN202421895161.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-05
- Publication Date
- 2025-08-12
- Estimated Expiration
- 2034-08-05
AI Technical Summary
Existing microscopic imaging devices often only offer a single imaging mode, resulting in researchers needing to switch between multiple devices, reducing sample analysis efficiency and increasing costs.
Design a microscopic imaging device that integrates quantitative phase imaging, fluorescence imaging and bright field imaging functions, and realizes switching of multiple imaging modes within the same device through the combination of multiple light sources and imaging modules.
Improve sample analysis efficiency, reduce costs, and reduce equipment volume and simplify operational processes.
Smart Images
Figure CN223217730U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of microscopic imaging, and in particular to a microscopic imaging device. Background Art
[0002] Microscopy is one of the most important tools in biomedical research and clinical diagnostics. To observe the structure, function, and dynamic changes of cells and tissues, researchers and clinicians rely on a variety of microscopic imaging methods to analyze cell or tissue samples, including but not limited to white-field imaging and fluorescence imaging.
[0003] Currently, the microscopic imaging equipment on the market often only provides a single imaging mode. This means that when researchers need to analyze samples based on multiple imaging results, they need to switch between multiple microscopic imaging devices that provide different imaging modes, resulting in low sample analysis efficiency and high sample analysis costs. Utility Model Content
[0004] The present application provides a microscopic imaging device, which aims to improve the sample analysis efficiency and reduce the sample analysis cost in microscopic imaging, as well as reduce the volume of the microscopic imaging device.
[0005] In a first aspect, the present application provides a microscopic imaging device, comprising:
[0006] base;
[0007] a support member, the support member being arranged on the base;
[0008] a light source assembly, the light source assembly being disposed on the top of the support member, the light source assembly being connected to the first light source, and the light source assembly including a second light source;
[0009] A loading platform, which is provided on the support member and is used to carry a sample to be observed;
[0010] An objective lens assembly, the objective lens assembly being arranged on the support member and located below the object-carrying platform;
[0011] a fluorescence imaging module, the fluorescence imaging module being disposed on the base and at least partially located below the objective lens assembly, the fluorescence imaging module comprising a fluorescence light source and a first camera;
[0012] A quantitative phase imaging module, the quantitative phase imaging module is arranged on the base and adjacent to the fluorescence imaging module;
[0013] Among them, the first light beam provided by the first light source is emitted from the light source assembly, and the first light beam is incident on the quantitative phase imaging module together with the second light beam provided by the first light source through the sample to be observed on the stage, and quantitative phase imaging of the sample to be observed is performed; the fluorescence excitation light beam provided by the fluorescence light source is projected onto the sample to be observed on the stage, and the corresponding fluorescence emission light beam is incident on the first camera, and fluorescence imaging of the sample to be observed is performed; and the third light beam provided by the second light source is incident on the first camera through the sample to be observed on the stage, and bright field imaging of the sample to be observed is performed.
[0014] In one embodiment, the microscopic imaging device further comprises: a bright field imaging module, the bright field imaging module being disposed on the base and adjacent to the fluorescence imaging module, the bright field imaging module comprising a second camera;
[0015] The third light beam provided by the second light source passes through the sample to be observed on the object platform and is incident on the second camera to perform bright field imaging on the sample to be observed.
[0016] In one embodiment, the bright field imaging module is disposed on the base adjacent to the quantitative phase imaging module; or
[0017] The bright field imaging module and the quantitative phase imaging module are respectively arranged on both sides of the fluorescence imaging module.
[0018] In one embodiment, the fluorescence imaging module further includes a plurality of fluorescence cubes and a moving component;
[0019] A plurality of the fluorescence cubes are arranged in parallel and are all connected to the fluorescence light source. When performing fluorescence imaging on the sample to be observed, the fluorescence light source provides a corresponding fluorescence excitation beam through the fluorescence cube, so that the fluorescence excitation beam is projected onto the sample to be observed on the object platform, and the corresponding fluorescence emission beam is incident on the first camera.
[0020] The moving component is disposed on the base, and the fluorescence cube is disposed on the moving component. The moving component is used to move the fluorescence cube to control the fluorescence cube to be aligned with the sample to be observed, thereby controlling the fluorescence excitation beam provided to the sample to be observed.
[0021] In one embodiment, the moving assembly includes a first slide rail and a second slide rail, the first slide rail and the second slide rail are disposed opposite to each other on the base, and both the first slide rail and the second slide rail are connected to the fluorescent cube.
[0022] In one embodiment, the microscopic imaging device includes an optical path module, and the optical path module is arranged on the base;
[0023] In which, the optical path module is used to transmit the first light beam passing through the sample to be observed on the object platform and the second light beam provided by the first light source to the quantitative phase imaging module, or to transmit the fluorescent emission light beam to the first camera, or to transmit the third light beam passing through the sample to be observed on the object platform to the first camera.
[0024] In one embodiment, the optical path module includes a dichroic mirror; when quantitative phase imaging is performed on the sample to be observed, a first light beam passing through the sample to be observed on the loading platform is incident on the quantitative phase imaging module after being refracted by the dichroic mirror, and a second light beam passes through the dichroic mirror from a first direction and is incident on the quantitative phase imaging module; when fluorescence imaging is performed on the sample to be observed, the fluorescence emission light beam passes through the dichroic mirror from a second direction and is incident on the first camera, and the first direction and the second direction are perpendicular to each other.
[0025] In one embodiment, the optical path module is at least partially disposed between the first slide rail and the second slide rail in the fluorescence imaging module.
[0026] In one embodiment, the second light source includes a first white light source and a second white light source, and the light source assembly further includes a beam splitter prism, a laser coupling assembly, and a polarization assembly;
[0027] The first white light source, the beam splitter prism and the second white light source are arranged in a first optical path, the first optical path is perpendicular to the object plane of the object platform, the beam splitter prism, the laser coupling component and the polarization component are arranged in a second optical path, the second optical path is perpendicular to the first optical path.
[0028] In one embodiment, the microscopic imaging device further includes a control module, which is disposed on the base. The fluorescence imaging module, the quantitative phase imaging module, and the control module are disposed around the support member.
[0029] The microscopic imaging device provided in the present application has a fluorescence imaging module and a quantitative phase imaging module on the base, so that the microscopic imaging device can provide users with quantitative phase imaging, fluorescence imaging and bright field imaging functions, and simplifies the switching operation between various imaging modes, so that users do not need to switch devices when multiple imaging is required; and the connections between the various components and modules on the microscopic imaging device are relatively tight, thereby reducing the space occupied by the microscopic imaging device. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.
[0031] Figure 1 A schematic structural diagram of a microscopic imaging device provided in an embodiment of the present application;
[0032] Figure 2 A front view of the microscopic imaging device provided in this application;
[0033] Figure 3 This is a schematic block diagram of the structure of a microscopic imaging device provided in one embodiment of the present application. DETAILED DESCRIPTION
[0034] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.
[0035] The flowcharts shown in the accompanying drawings are for illustrative purposes only and do not necessarily include all contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may be decomposed, combined, or partially merged, so the actual execution order may vary depending on the actual situation.
[0036] The following describes some embodiments of the present application in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.
[0037] Please refer to Figure 1 , Figure 1 A schematic structural diagram of a microscopic imaging device provided in an embodiment of the present application.
[0038] like Figure 1As shown, the microscopic imaging device includes a base 10, a support 20, a light source assembly 30, a stage 40, an objective lens assembly 50, a fluorescence imaging module 60, and a quantitative phase imaging module 70. The support 20 is disposed on the base 10, and the light source assembly 30 is disposed on top of the support 20. The light source assembly 30 is connected to a first light source and includes a second light source. The stage 40 and the objective lens assembly 50 are both disposed on the support 20. The stage 40 is used to carry a sample to be observed, and the objective lens assembly 50 is disposed below the stage 40. The fluorescence imaging module 60 and the quantitative phase imaging module 70 are both disposed on the base 10. The fluorescence imaging module 60 includes a fluorescence light source 61 and a first camera 62, and is at least partially located below the objective lens assembly 50. The quantitative phase imaging module 70 is disposed adjacent to the fluorescence imaging module 60.
[0039] The microscopic imaging device is capable of performing quantitative phase imaging, fluorescence imaging, or brightfield imaging of the sample to be observed. Specifically, a first light beam provided by a first light source is emitted from the light source assembly 30. The first light beam passes through the sample to be observed placed on the stage 40 and, together with a second light beam provided by the first light source, is incident on the quantitative phase imaging module 70 to perform quantitative phase imaging of the sample to be observed. It is understood that a coupler can be provided in the first light source or in the light output path of the first light source to separate the light beam provided by the first light source into a first light beam and a second light beam. The first light beam and the second light beam are coherent light required for quantitative phase imaging of the sample to be observed. During fluorescence imaging of the sample to be observed, a fluorescence excitation beam provided by the fluorescence light source 61 is projected onto the sample to be observed on the stage 40. After obtaining a fluorescence emission beam corresponding to the fluorescence excitation beam, the fluorescence emission beam is incident on the first camera 62 to achieve fluorescence imaging of the sample to be observed. During brightfield imaging of the sample to be observed, a third light beam provided by the second light source is transmitted through the sample to be observed on the stage 40 and is incident on the first camera 62 to achieve brightfield imaging of the sample to be observed.
[0040] By using multiple light sources and corresponding imaging modules to meet different imaging needs, the microscopic imaging device can switch between different imaging modes, reducing the cost of multiple imaging and the dependence of different imaging modes on multiple devices. Different imaging modes can be switched on the same device to obtain more comprehensive information about the sample to be observed without having to place the sample to be observed on another device for another imaging method. This simplifies the imaging processing operation of the same sample to be observed and improves the efficiency of sample imaging and analysis.
[0041] See also Figure 2 , Figure 2 This is a front view of the microscopic imaging device provided in this application.
[0042] In a specific embodiment, in the forward-looking direction of the microscopic imaging device, the length of the loading platform 40 is smaller than the length of the base 10, so that the microscopic imaging device has a "earth"-shaped structure, which facilitates the installation of the fluorescence imaging module 60 and the quantitative phase imaging module 70 on the microscopic imaging device, increases the stability of the microscopic imaging device, and reduces the space occupied by the microscopic imaging device.
[0043] In some embodiments, the microscopic imaging device also includes: a bright field imaging module 80, which is arranged on the base 10 and adjacent to the fluorescence imaging module 60, and the bright field imaging module 80 includes a second camera; wherein the third light beam provided by the second light source passes through the sample to be observed on the loading platform 40 and is incident on the second camera to perform bright field imaging of the sample to be observed.
[0044] It should be noted that when achieving brightfield imaging, the first camera 62 can capture the third light beam that passes through the sample to be observed to perform brightfield imaging of the sample to be observed. A brightfield imaging module 80 including a second camera can also be provided on the base 10 of the microscopic imaging device, so that the third light beam provided by the second light source passes through the sample to be observed on the sample loading platform 40 and is incident on the second camera, thereby achieving brightfield imaging of the sample to be observed. The first camera 62 is only used for fluorescence imaging of the sample to be observed, thereby improving imaging quality in different imaging modes.
[0045] In some embodiments, the bright field imaging module 80 is disposed on the base 10 adjacent to the quantitative phase imaging module 70 .
[0046] Exemplarily, the bright field imaging module 80 and the quantitative phase imaging module 70 are both arranged on the same side of the fluorescence imaging module 60 , and the other side of the fluorescence imaging module 60 can be provided with other modules or used to place required objects according to actual needs.
[0047] See also Figure 3 , Figure 3 This is a schematic block diagram of the structure of a microscopic imaging device provided in one embodiment of the present application.
[0048] In other embodiments, the bright field imaging module 80 and the quantitative phase imaging module 70 are respectively disposed on two sides of the fluorescence imaging module 60 .
[0049] It should be understood that without setting up other modules, the bright field imaging module 80 and the quantitative phase imaging module 70 are respectively arranged on both sides of the fluorescence imaging module 60 to make the microscopic imaging device more compact, thereby reducing the space occupied by the microscopic imaging device.
[0050] In some embodiments, the fluorescence imaging module 60 further includes a plurality of fluorescence cubes and a movable component; the plurality of fluorescence cubes are arranged in parallel and are all connected to the fluorescence light source 61. When performing fluorescence imaging on the sample to be observed, the fluorescence light source provides a corresponding fluorescence excitation beam through the fluorescence cube, so that the fluorescence excitation beam is projected onto the sample to be observed on the loading platform 40, and the corresponding fluorescence emission beam is incident on the first camera 62; the movable component is arranged on the base 10, and the fluorescence cube is arranged on the movable component. The movable component is used to move the fluorescence cube to control the fluorescence cube aligned with the sample to be observed, and thereby control the fluorescence excitation beam provided to the sample to be observed.
[0051] Exemplarily, the movable component in the fluorescence imaging module 60 is disposed on the base 10, and each fluorescence cube is connected to a corresponding fluorescence light source 61 to provide fluorescence excitation light of different wavelength ranges to the sample to be observed. Multiple fluorescence cubes are arranged in parallel on the movable component so that the movable component can move the fluorescence cube, thereby controlling the fluorescence cube aligned vertically with the sample to be observed, and further controlling the fluorescence excitation light provided to the sample to be observed.
[0052] It should be understood that during the fluorescence imaging process, the fluorescence light source 61 provides a fluorescence excitation beam of a specific wavelength through a fluorescence cube aligned with the sample to be observed, so that after the fluorescence excitation beam is irradiated onto the sample to be observed, the corresponding fluorescence emission beam is reflected back and incident on the first camera 62, so that the corresponding fluorescence signal can be collected in the first camera 62, thereby realizing fluorescence imaging of the sample to be observed.
[0053] In a specific implementation, at least three fluorescence cubes are provided to provide fluorescence excitation beams of different wavelengths to the sample to be observed. When performing brightfield imaging on the sample to be observed, the movable assembly can also move the fluorescence cubes so that they are idle. This allows the third beam provided by the second light source to bypass the fluorescence cubes and enter the first camera 62 for brightfield imaging of the sample to be observed. Specifically, the movable assembly moves the fluorescence cubes to adjust the alignment of the fluorescence cube with the sample to be observed or to idle the fluorescence cubes for corresponding fluorescence imaging or brightfield imaging.
[0054] In some embodiments, the moving assembly includes a first slide rail and a second slide rail, the first slide rail and the second slide rail are disposed opposite to each other on the base 10, and both the first slide rail and the second slide rail are connected to the fluorescent cube.
[0055] Illustratively, the first slide rail and the second slide rail are arranged opposite to each other on the base 10, and a plurality of fluorescence cubes are arranged in parallel above the first slide rail and the second slide rail, so that the fluorescence cube can slide on the first slide rail and the second slide rail to switch the fluorescence cube aligned with the sample to be observed or to put the fluorescence cube in an idle state, thereby performing corresponding fluorescence imaging or bright field imaging of the sample to be observed.
[0056] In a specific embodiment, corresponding drive components can be provided to drive the movement of the fluorescence cube along the first and second rails. This application does not limit the specific driving method of the fluorescence cube. The provision of the first and second rails facilitates switching the fluorescence cube aligned with the sample to be observed, thereby improving the efficiency of fluorescence imaging and the efficiency of switching between fluorescence and brightfield imaging modes.
[0057] In some embodiments, the microscopic imaging device includes an optical path module, which is disposed on the base 10; wherein the optical path module is used to transmit a first light beam passing through the sample to be observed on the loading platform 40 and a second light beam provided by the first light source to the quantitative phase imaging module 70, or to transmit the fluorescent emission light beam to the first camera 62, or to transmit a third light beam passing through the sample to be observed on the loading platform 40 to the first camera 62.
[0058] Exemplarily, an optical path module is also provided on the base 10 of the microscopic imaging device, which is used to shorten the optical path of the light beam passing through the sample to be observed on the loading platform 40 to the corresponding quantitative phase imaging module 70 or the first camera 62, thereby making the microscopic imaging device more compact and reducing the volume and space occupied by the microscopic imaging device.
[0059] In the specific implementation process, since the light beam needs to pass through the sample to be observed on the loading platform 40 or reflect the fluorescent emission light beam from the sample to be observed, the light beam that needs to be incident on the quantitative phase imaging module 70 or the first camera 62 is propagated in a plane perpendicular to the base 10. By setting the optical path module, the light beam can change the propagation direction after being vertically incident on the optical path module and propagate in a direction parallel to the plane of the base 10, so that it is received by the quantitative phase imaging module 70 or the first camera 62 arranged on the base 10, thereby realizing imaging of the sample to be observed.
[0060] In some embodiments, the optical path module is at least partially disposed between the first slide rail and the second slide rail in the fluorescence imaging module 60 .
[0061] Exemplarily, the optical path module is at least partially disposed between the first slide rail and the second slide rail in the fluorescence imaging module 60, so that the light beam passing through the sample to be observed or the fluorescence emission light beam reflected from the sample to be observed can be incident on the optical path module and have its propagation direction changed by the optical path module so that it can finally be incident on the corresponding quantitative phase imaging module 70 or the first camera 62.
[0062] It should be noted that this application does not limit the setting of optical elements such as lenses or reflectors in the optical path module. Those skilled in the art can set corresponding lenses or reflectors according to actual optical path design requirements to achieve the design or adjustment of the optical path module.
[0063] In some embodiments, the optical path module includes a dichroic mirror; when performing quantitative phase imaging on the sample to be observed, the first light beam of the sample to be observed on the loading platform 40 is incident on the quantitative phase imaging module 70 after being refracted by the dichroic mirror; when performing fluorescence imaging on the sample to be observed, the fluorescence emission light beam passes through the dichroic mirror and is incident on the first camera 62.
[0064] Exemplarily, a dichroic mirror is provided in the optical path module. When performing quantitative phase imaging on the sample to be observed, the first light beam emitted from the light source assembly 30 passes through the sample to be observed on the sample platform 40, is refracted by the dichroic mirror, and is incident on the quantitative phase imaging module 70. Together with the second light beam, it provides the coherent light required for quantitative phase imaging (QPI). The coherent light is received by the quantitative phase imaging module 70 to perform quantitative phase imaging on the sample to be observed. It should be noted that the first light source is a laser light source, and therefore the first and second light beams are both laser beams. During the quantitative phase imaging process, the dichroic mirror can improve the light efficiency of the optical path module, thereby improving the quality of quantitative phase imaging.
[0065] When fluorescence imaging is performed on the sample to be observed, the fluorescence emission beam corresponding to the fluorescence excitation beam passes through the dichroic mirror and is incident on the first camera 62 to achieve fluorescence imaging of the sample to be observed; and when bright field imaging is performed on the sample to be observed, the third light beam passing through the sample to be observed also passes through the dichroic mirror and is incident on the first camera 62.
[0066] In some embodiments, the second light source includes a first white light source and a second white light source, and the light source assembly 30 further includes a beam splitter, a laser coupling assembly and a polarization assembly; the first white light source, the beam splitter and the second white light source are arranged in a first optical path, the first optical path is perpendicular to the loading plane of the loading platform 40, the beam splitter, the laser coupling assembly and the polarization assembly are arranged in a second optical path, and the second optical path is perpendicular to the first optical path.
[0067] Exemplarily, the laser coupling assembly is connected to one end of the optical fiber, and the other end of the optical fiber is connected to the first light source, so that the first light beam of the first light source can be emitted from the light source assembly 30. In some specific embodiments, the first light source is arranged on the base 10 or in the quantitative phase imaging module 70; the second light source includes a first white light source and a second white light source, wherein the first white light source is used to provide the light beam required for bright field imaging, and the second white light source is used as an auxiliary light source to supplement the light of the first white light source or provide illumination at different angles to improve the imaging quality, so that the microscopic imaging equipment can adapt to a wide range of observation and imaging applications.
[0068] In a specific embodiment, the light source assembly 30 also includes at least a beam splitter prism and a polarization assembly, wherein the first white light source, the beam splitter prism and the second white light source are sequentially arranged in a first optical path, and the first optical path is perpendicular to the loading plane of the loading platform 40, so that the third light beam emitted from the first white light source can illuminate the sample to be observed on the loading platform 40; the beam splitter prism, the polarization assembly and the laser coupling assembly are sequentially arranged in a second optical path perpendicular to the first optical path. It should be understood that the first light beam emitted from the laser coupling assembly is incident on the beam splitter prism through the polarization assembly and is refracted by the beam splitter prism, thereby irradiating the sample to be observed on the loading platform 40.
[0069] Exemplarily, the first white light source is a cylindrical light source, and the second white light source is a ring-shaped light source.
[0070] In some embodiments, the microscopic imaging device further includes a control module, which is disposed on the base 10 . The fluorescence imaging module 60 , the quantitative phase imaging module 70 , and the control module are disposed around the support 20 .
[0071] Exemplarily, the control module includes but is not limited to controlling the working status of each light source, and / or the target objective lens used in the imaging process in the objective lens assembly 50, wherein, the technician using the microscopic imaging equipment can control the modules in the microscopic imaging equipment through the control module that communicates with the control module, thereby achieving effects such as switching imaging modes or switching objective lenses, without the need for manual control adjustment, so as to improve the control accuracy and imaging quality of the microscopic imaging equipment.
[0072] In a specific implementation, support member 20 is positioned in the central region of base 10. The central region is defined as a region within a predetermined range centered on the center point of base 10. The specific range can be determined based on the area of base 10 and is not limited by this application. Fluorescence imaging module 60, quantitative phase imaging module 70, and control module are positioned around support member 20, making the microscopic imaging apparatus more compact and further reducing the space occupied by the microscopic imaging apparatus.
[0073] The microscopic imaging device provided in the above embodiment can provide users with quantitative phase imaging, fluorescence imaging and bright field imaging functions, so that users do not need to switch devices when they need to perform multiple imaging operations; and the method of switching imaging is relatively convenient, which improves imaging efficiency; and the connections between the various components and modules on the microscopic imaging device are relatively tight, thereby reducing the volume and space occupied by the microscopic imaging device.
[0074] It should be understood that the terms used in this specification are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.
[0075] It should also be understood that the term "and / or" used in this specification and the appended claims refers to any combination of one or more of the associated listed items and all possible combinations, including these combinations. It should be noted that, in this article, the terms "include", "comprise" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or system that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article or system. In the absence of further restrictions, an element defined by the sentence "including a..." does not exclude the presence of other identical elements in the process, method, article or system that includes the element.
[0076] The serial numbers of the embodiments of the present application are for description only and do not represent the advantages or disadvantages of the embodiments. The above description is only a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in this application, and these modifications or replacements should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.
Claims
1. A microscopic imaging device, characterized in that: The microscopic imaging device comprises: base; a support member, the support member being arranged on the base; a light source assembly, the light source assembly being disposed on the top of the support member, the light source assembly being connected to the first light source, and the light source assembly including a second light source; A loading platform, which is provided on the support member and is used to carry a sample to be observed; An objective lens assembly, the objective lens assembly being arranged on the support member and located below the object-carrying platform; a fluorescence imaging module, the fluorescence imaging module being disposed on the base and at least partially located below the objective lens assembly, the fluorescence imaging module comprising a fluorescence light source and a first camera; A quantitative phase imaging module, the quantitative phase imaging module is arranged on the base and adjacent to the fluorescence imaging module; Among them, the first light beam provided by the first light source is emitted from the light source assembly, and the first light beam is incident on the quantitative phase imaging module together with the second light beam provided by the first light source through the sample to be observed on the stage, and quantitative phase imaging of the sample to be observed is performed; the fluorescence excitation light beam provided by the fluorescence light source is projected onto the sample to be observed on the stage, and the corresponding fluorescence emission light beam is incident on the first camera, and fluorescence imaging of the sample to be observed is performed; and the third light beam provided by the second light source is incident on the first camera through the sample to be observed on the stage, and bright field imaging of the sample to be observed is performed.
2. The microscopic imaging device according to claim 1, wherein The microscopic imaging device further includes: a bright field imaging module, the bright field imaging module being disposed on the base and adjacent to the fluorescence imaging module, the bright field imaging module including a second camera; The third light beam provided by the second light source passes through the sample to be observed on the object platform and is incident on the second camera to perform bright field imaging on the sample to be observed.
3. The microscopic imaging device according to claim 2, wherein: The bright field imaging module is disposed on the base adjacent to the quantitative phase imaging module; or The bright field imaging module and the quantitative phase imaging module are respectively arranged on both sides of the fluorescence imaging module.
4. The microscopic imaging device according to any one of claims 1 to 3, wherein: The fluorescence imaging module also includes a plurality of fluorescence cubes and a moving component; A plurality of the fluorescence cubes are arranged in parallel and are all connected to the fluorescence light source. When performing fluorescence imaging on the sample to be observed, the fluorescence light source provides a corresponding fluorescence excitation beam through the fluorescence cube, so that the fluorescence excitation beam is projected onto the sample to be observed on the object platform, and the corresponding fluorescence emission beam is incident on the first camera. The moving component is disposed on the base, and the fluorescence cube is disposed on the moving component. The moving component is used to move the fluorescence cube to control the fluorescence cube to be aligned with the sample to be observed, thereby controlling the fluorescence excitation beam provided to the sample to be observed.
5. The microscopic imaging device according to claim 4, wherein: The moving assembly includes a first slide rail and a second slide rail. The first slide rail and the second slide rail are arranged opposite to each other on the base, and both the first slide rail and the second slide rail are connected to the fluorescent cube.
6. The microscopic imaging device according to claim 1, wherein: The microscopic imaging device includes an optical path module, and the optical path module is arranged on the base; In which, the optical path module is used to transmit the first light beam passing through the sample to be observed on the object platform and the second light beam provided by the first light source to the quantitative phase imaging module, or to transmit the fluorescent emission light beam to the first camera, or to transmit the third light beam passing through the sample to be observed on the object platform to the first camera.
7. The microscopic imaging device according to claim 6, wherein: The optical path module includes a dichroic mirror; when quantitative phase imaging is performed on the sample to be observed, a first light beam passing through the sample to be observed on the loading platform is refracted by the dichroic mirror and incident on the quantitative phase imaging module; when fluorescence imaging is performed on the sample to be observed, the fluorescence emission light beam passes through the dichroic mirror and is incident on the first camera.
8. The microscopic imaging device according to claim 6, wherein: The optical path module is at least partially arranged between the first slide rail and the second slide rail in the fluorescence imaging module.
9. The microscopic imaging device according to any one of claims 1 to 3, wherein: The second light source includes a first white light source and a second white light source, and the light source assembly further includes a beam splitter prism, a laser coupling assembly, and a polarization assembly; The first white light source, the beam splitter prism and the second white light source are arranged in a first optical path, the first optical path is perpendicular to the object plane of the object platform, the beam splitter prism, the laser coupling component and the polarization component are arranged in a second optical path, the second optical path is perpendicular to the first optical path.
10. The microscopic imaging device according to claim 1, wherein: The microscopic imaging device further includes a control module, which is disposed on the base. The fluorescence imaging module, the quantitative phase imaging module, and the control module are disposed around the support member.