Novel cell scratching device

Abstract

The utility model provides a novel cell scratching device, which belongs to the field of experimental equipment and comprises a detachable top cover arranged at the top of a six-hole plate, a scratching mechanism is arranged in the top cover, and the scratching mechanism comprises a corresponding groove, a moving groove, a moving seat, a bearing, a moving rod, an interference groove, a two-way screw rod, a telescopic rod, a buffer spring and a scratching needle. The position of the moving rod can be adjusted, then the scratching needle at the bottom is driven to move to a proper position, or the two-way screw can be rotated, the telescopic rod on the two-way screw can be adjusted more accurately, the angle of the moving rod is adjusted by rotating the moving rod, and meanwhile under the action of the buffer spring, the scratching needle is driven to move to a proper position. The length of the scratching needle can automatically adapt to length changes caused by angle changes, then the moving rod on the outer side is pulled, scratching work is completed, stress is uniform, and the width of scratches is more uniform.

Description

Technical Field

[0001] The utility model relates to the field of experimental equipment, and in particular to a novel cell scratching device. Background Art

[0002] The scratch assay, also known as the wound healing assay, is used to test the migration ability of cells. Its principle is based on the ability of cells to repair wounds or injuries in an in vitro culture environment. By creating artificial scratches or wounds on a monolayer of adherent cells in cell culture, and then observing how the cells migrate and fill these scratched areas, the migration and healing abilities of cells are evaluated. However, the six-well plate used in cell experiments, which is a common equipment for scratch assays, still has the following drawbacks when used:

[0003] Existing devices require manual positioning during the experiment, which is time-consuming and labor-intensive. They also require manual scratching, which can easily lead to uneven force, scratches of varying widths and paths, and increase experimental errors.

[0004] Therefore, we made improvements and proposed a new cell scratching device. Utility Model Content

[0005] The purpose of the utility model is to address the current problem that manual positioning is required during the experiment, which is time-consuming and labor-intensive, and also requires manual scratching, but is prone to uneven force, scratches of varying widths and paths, and easily increases experimental errors.

[0006] In order to achieve the above-mentioned purpose of the utility model, the utility model provides the following technical solutions:

[0007] A new cell scratching device is developed to improve the above problems.

[0008] The utility model is specifically as follows:

[0009] It comprises a six-well plate, the top of which is provided with a detachable top cover, and the interior of the top cover is provided with a scratching mechanism;

[0010] The scratching mechanism includes a corresponding groove, a movable groove, a movable seat, a bearing, a movable rod, an interference groove, a bidirectional screw, a telescopic rod, a buffer spring and a scratching needle. The corresponding groove is opened in the interior of the top cover, the movable groove is opened in the side wall of the top cover, the movable seat is slidably connected to the movable groove, the bearing is rotatably connected to the interior of the movable seat, the movable rod is arranged in the corresponding groove and both ends are located inside the bearing, the interference groove is opened in part of the side wall of the movable rod, the bidirectional screw is rotatably arranged inside the movable rod, the telescopic rod is connected to the bidirectional screw by a thread and is located at the position of the interference groove, the buffer spring is sleeved on the telescopic rod, and the scratching needle is fixedly arranged at one end of the bottom of the telescopic rod.

[0011] As a preferred technical solution of the present invention, a detachable bottom groove is provided at the bottom of the six-hole plate, and the surface of the bottom groove is evenly marked with lines for scratching and positioning.

[0012] As a preferred technical solution of the present invention, a corrugated plate is fixedly provided inside the movable groove, and the corrugated plate is located on both sides of the movable seat to fill the movable groove.

[0013] As a preferred technical solution of the present invention, a first guide groove is provided on the movable rod at an inner side of the interference groove, and the first guide groove cooperates with the top of the telescopic rod.

[0014] As a preferred technical solution of the present invention, the corresponding grooves correspond one-to-one with the hole grooves of the six-hole plate.

[0015] As a preferred technical solution of the present invention, a second guide groove is provided on the inner top of the movable groove, and the second guide groove cooperates with the top of the movable seat.

[0016] As a preferred technical solution of the present invention, the inner wall of the bearing is square and matches the cross-sectional shape of the moving rod. The moving rod can move in the bearing and can drive the bearing to rotate through the square inner wall.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In the solution of the present utility model:

[0019] Through the set scratching mechanism, during use, the position of the moving rod can be adjusted, and then the scratching needle at the bottom can be driven to move to the appropriate position, or the bidirectional screw can be rotated to adjust the telescopic rod on the bidirectional screw more accurately. The angle of the moving rod can be adjusted by rotating the moving rod. At the same time, under the action of the buffer spring, the length of the scratching needle will automatically adapt to the length change caused by the angle change, and then the outer moving rod is pulled to complete the scratching work, so that the force is even and the width of the scratch is more uniform. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic structural diagram of the novel cell scratching device provided by the utility model;

[0021] Figure 2 This is a schematic diagram of the overall cross-sectional structure of the novel cell scratching device provided by the utility model;

[0022] Figure 3 This is a schematic diagram of the overall explosion structure of the novel cell scratching device provided by the utility model;

[0023] Figure 4A schematic diagram of the partial structure of the scratching mechanism of the novel cell scratching device provided by the present invention;

[0024] Figure 5 This is a schematic structural diagram of the first guide groove and the second guide groove of the novel cell scratching device provided by the utility model.

[0025] Indicated in the figure:

[0026] 1. Six-hole plate; 2. Top cover; 3. Scratching mechanism; 301. Corresponding groove; 302. Moving groove; 303. Moving seat; 304. Bearing; 305. Moving rod; 306. Interference groove; 307. Bidirectional screw; 308. Telescopic rod; 309. Buffer spring; 310. Scratching needle; 4. Bottom groove; 5. Corrugated plate; 6. First guide groove; 7. Second guide groove. DETAILED DESCRIPTION

[0027] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be described clearly and completely in conjunction with the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them.

[0028] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents some embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0029] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions therein can be combined with each other.

[0030] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0031] like Figure 1-5 As shown, this embodiment proposes a novel cell scratching device, comprising a six-well plate 1, a detachable top cover 2 is provided on the top of the six-well plate 1, and a scratching mechanism 3 is provided inside the top cover 2;

[0032] like Figure 2 and Figure 4As shown, the scratching mechanism 3 includes a corresponding groove 301, a moving groove 302, a moving seat 303, a bearing 304, a moving rod 305, an interference groove 306, a bidirectional screw 307, a telescopic rod 308, a buffer spring 309 and a scratching needle 310, the corresponding groove 301 is opened in the interior of the top cover 2, the moving groove 302 is opened in the side wall of the top cover 2, the moving seat 303 is slidably connected to the moving groove 302, the bearing 304 is rotatably connected to the interior of the moving seat 303, the moving rod 305 is arranged in the corresponding groove 301 and both ends are located inside the bearing 304, the interference groove 306 is opened in part of the side wall of the moving rod 305, the bidirectional screw 307 is rotatably arranged inside the moving rod 305, and the telescopic rod 308 is threadedly connected to the bidirectional screw 307 and is located at the position of the interference groove 306 The buffer spring 309 is sleeved on the telescopic rod 308, and the scratching needle 310 is fixedly set at the bottom end of the telescopic rod 308. When in use, first adjust the position of the moving rod 305 to make a rough adjustment to the scratching needle 310, and then the bidirectional screw 307 can be rotated. Since the telescopic rod 308 on the bidirectional screw 307 is symmetrically distributed, the telescopic rod 308 performs synchronous relative movement or opposite movement through the bidirectional thread on the bidirectional screw 307. After adjusting to the appropriate position, rotate the moving rod 305 to a suitable angle to adjust the width of the scratch. The scratch is thinnest when vertical. The length of the scratching needle 310 will automatically adapt to the length change caused by the angle change due to the action of the buffer spring 309, and then pull the end of the external moving rod 305 to form a scratch.

[0033] like Figure 3 As shown, a detachable bottom groove 4 is provided at the bottom of the six-hole plate 1, and the surface of the bottom groove 4 is evenly marked with lines for scratching and positioning.

[0034] like Figure 1 and Figure 2 As shown, a corrugated plate 5 is fixedly provided inside the movable groove 302. The corrugated plate 5 is located on both sides of the movable seat 303 to fill the movable groove 302 and fill the rest of the movable groove 302 to protect the interior without affecting the scratches.

[0035] like Figure 5 As shown, a first guide groove 6 is provided on the inner side of the interference groove 306 on the movable rod 305. The first guide groove 6 cooperates with the top of the telescopic rod 308. Due to the limitation of the first guide groove 6 and the top of the telescopic rod 308, the stable movement of the telescopic rod 308 is guaranteed.

[0036] like Figure 4 As shown, the corresponding grooves 301 correspond one-to-one with the hole grooves of the six-hole plate 1, which can ensure the accuracy of scratching.

[0037] like Figure 5As shown, a second guide groove 7 is provided at the inner top of the movable groove 302 , and the second guide groove 7 cooperates with the top of the movable seat 303 to ensure that the movable seat 303 does not deviate.

[0038] like Figure 2 、 Figure 4 and Figure 5 As shown, the inner wall of the bearing 304 is square and matches the cross-sectional shape of the moving rod 305 . The moving rod 305 can move in the bearing 304 and can drive the bearing 304 to rotate through the square inner wall.

[0039] Specifically, when the new cell scratching device is in operation: assemble the top cover 2 and the bottom groove 4, adjust the position of the moving rod 305 to make a rough adjustment to the scratching needle 310, then rotate the bidirectional screw 307, and the telescopic rod 308 performs synchronous relative movement or opposite movement through the bidirectional thread on the bidirectional screw 307. After adjusting to the appropriate position, rotate the moving rod 305 to a suitable angle to adjust the width of the scratch. The scratch is thinnest when vertical. The length of the scratching needle 310 will automatically adapt to the length change caused by the angle change due to the action of the buffer spring 309, and then pull the end of the external moving rod 305 to form a scratch.

[0040] All technical features in this embodiment can be freely combined according to actual needs.

[0041] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present technical solution is within the scope of protection of the present invention.

Claims

1. A novel cell scratching device, comprising a six-well plate (1), characterized in that: A detachable top cover (2) is provided on the top of the six-well plate (1), and a scratching mechanism (3) is provided inside the top cover (2); The scratching mechanism (3) comprises a corresponding groove (301), a movable groove (302), a movable seat (303), a bearing (304), a movable rod (305), an interference groove (306), a bidirectional screw (307), a telescopic rod (308), a buffer spring (309) and a scratching needle (310), wherein the corresponding groove (301) is provided inside the top cover (2), the movable groove (302) is provided on the side wall of the top cover (2), the movable seat (303) is slidably connected to the movable groove (302), and the bearing (304) is rotatably connected to the movable seat (303). The movable rod (305) is arranged in the corresponding groove (301) and both ends are located inside the bearing (304). The interference groove (306) is opened on a part of the side wall of the movable rod (305). The bidirectional screw (307) is rotatably arranged inside the movable rod (305). The telescopic rod (308) is connected to the bidirectional screw (307) by a thread and is located at the position of the interference groove (306). The buffer spring (309) is sleeved on the telescopic rod (308). The scratching needle (310) is fixedly arranged at one end of the bottom of the telescopic rod (308).

2. A novel cell scratching device according to claim 1, characterized in that: The bottom of the six-hole plate (1) is provided with a detachable bottom groove (4), and the surface of the bottom groove (4) is evenly marked with lines for scratching and positioning.

3. A novel cell scratching device according to claim 1, characterized in that: A corrugated plate (5) is fixedly provided inside the movable groove (302), and the corrugated plate (5) is located on both sides of the movable seat (303) to fill the movable groove (302).

4. A novel cell scratching device according to claim 1, characterized in that: A first guide groove (6) is provided on the inner side of the interference groove (306) of the moving rod (305), and the first guide groove (6) cooperates with the top of the telescopic rod (308).

5. A novel cell scratching device according to claim 1, characterized in that: The corresponding grooves (301) correspond one-to-one to the hole grooves of the six-hole plate (1).

6. A novel cell scratching device according to claim 1, characterized in that: A second guide groove (7) is provided on the inner top of the movable groove (302), and the second guide groove (7) cooperates with the top of the movable seat (303).

7. A novel cell scratching device according to claim 1, characterized in that: The inner wall of the bearing (304) is square and matches the cross-sectional shape of the moving rod (305). The moving rod (305) can move in the bearing (304) and can drive the bearing (304) to rotate through the square inner wall.

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