Protein filtering device for molecular experiment

Abstract

The utility model discloses a protein filtering device for molecular experiments, and relates to the technical field of molecular experiments, the protein filtering device comprises a bottom plate, one side of the top end of the bottom plate is fixedly connected with a crushing device, the crushing device comprises a tank body, and the inner wall of the tank body is rotatably connected with a first rotating shaft; one end of the first rotating shaft is fixedly connected with a first bevel gear, the outer surface of the first bevel gear is in meshed connection with two second bevel gears, one end of each second bevel gear is fixedly sleeved with a second rotating shaft, and the outer surfaces of the first rotating shaft and the two second rotating shafts are jointly and movably connected with a fixed box in a penetrating mode; according to the protein raw material crushing device, raw materials can be crushed, the raw materials and water can be conveniently mixed to obtain protein raw materials, and the filtering assembly can be detached and cleaned.

Description

Technical Field

[0001] The utility model relates to the technical field of molecular experiments, in particular to a protein filtering device for molecular experiments. Background Art

[0002] In molecular biology and biochemistry, the quality of biomolecules has a significant impact on the accuracy and significance of experimental results. The degradation of nucleic acids and proteins by nucleases and proteases, respectively, is the main cause of signal loss or poor reaction yields in experiments. In the amino acid production process, the macromolecular proteins in the fermentation broth containing amino acids need to be filtered to obtain a pure amino acid solution. Therefore, protein filtration devices are indispensable for molecular experiments.

[0003] However, the prior art still has the following problems:

[0004] First, in the experiment, the raw materials need to be crushed and mixed with water to obtain protein raw materials. However, the existing raw material production equipment on the market cannot effectively crush the raw materials and mix them with water to obtain protein raw materials, and its practicality is low.

[0005] Secondly, most of the existing filter devices on the market are directly fixed inside the equipment, and the filter devices cannot be effectively disassembled for cleaning. The installation and disassembly are very inconvenient, which affects the use.

[0006] In response to the above problems, the inventors proposed a protein filtration device for molecular experiments to solve the above problems. Utility Model Content

[0007] In order to solve the problems of being unable to effectively crush the raw materials and mix them with water to obtain protein raw materials and being unable to effectively disassemble the filtering device for cleaning and installation; the purpose of the utility model is to provide a protein filtering device for molecular experiments.

[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions: a protein filtering device for molecular experiments, comprising a bottom plate, the top of the bottom plate is fixedly connected to a pump body, the top of the bottom plate is fixedly connected to a box body, the top of the bottom plate is fixedly connected to a filter assembly, the top side of the bottom plate is fixedly connected to a crushing device, the crushing device comprises a tank body, the bottom end of the tank body is fixedly connected to the top side of the bottom plate, the output end of the pump body is fixedly connected to a second connecting pipe, one end of the second connecting pipe is fixedly connected to and penetrates the outer surface of the tank body, the inner wall of the tank body is rotatably connected to a first rotating shaft, the two ends of the first rotating shaft are respectively rotatably connected to the inner walls of the two sides of the tank body, the outer surface of the tank body is fixedly connected to a first motor, the output end of the first motor penetrates the outer surface of the tank body and is fixedly connected to one end of the first rotating shaft, The two gears are connected with each other through the gear train of the first gear and the gear train is connected with the gear train of the second gear, and the two gear trains are connected with each other through the gear train of the second gear.

[0009] The filter assembly of claim 1, wherein both ends of the box body, the input end of the pump body, and one end of the box body are fixedly penetrated by a first connecting pipe, and the outer surfaces of the four second connecting pipes are sleeved with a sealing ring, one end of which is provided with a sealing groove, and one end of the other sealing ring is fixedly connected to a sealing ring used to cooperate with the sealing groove. The outer surfaces of the four sealing rings are fixedly connected to a fixing plate, and one end of the opposite surfaces of each two adjacent fixing plates is jointly provided with a sealing gasket, and one end of the two sealing rings is respectively butted, and the sealing gasket is placed between the two fixing plates. The sealing ring enters the inner wall of the sealing groove and cooperates with the sealing gasket to prevent water leakage, and each two adjacent fixing plates and one end of the sealing gasket are jointly penetrated and connected with four stud bolts distributed in a circular array, and the outer surfaces of the four stud bolts are threadedly connected with two nuts, the inner wall of the box body is fixedly connected to a plurality of card plates, and the inner wall of the box body is provided with a plurality of placement plates distributed at equal intervals, and the inner walls of the plurality of placement plates are fixedly connected to the filter screen.

[0010] Compared with the prior art, the beneficial effects of the present invention are:

[0011] 1. The utility model can drive the second rotating shaft to rotate respectively by the rotation of the two second bevel gears, and the rotation of the two second rotating shafts drives the blades to rotate, thereby achieving the purpose of effectively crushing the raw material and mixing it with water to obtain the protein raw material;

[0012] 2. The utility model can effectively and quickly disassemble, clean and install the filter assembly by placing the sealing gasket between the two fixing plates, inserting four stud bolts between the sealing gasket and the two fixing plates, and installing nuts on both ends of the stud bolts. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0014] Figure 1 This is a schematic diagram of the structure of the utility model.

[0015] Figure 2 This is a schematic diagram of the crushing device of the utility model.

[0016] Figure 3 This is a schematic diagram of the filter assembly of the present invention.

[0017] Figure 4 This is a partial structural diagram of the filter component of the utility model.

[0018] In the figure: 1. bottom plate; 2. pump body; 3. second connecting pipe; 4. filter assembly; 5. crushing device; 6. box body; 7. filter plate; 8. discharge port; 9. valve; 401. box body; 402. first connecting pipe; 403. sealing ring; 404. fixing plate; 405. sealing gasket; 406. stud bolt; 407. nut; 408. sealing groove; 409. sealing ring; 410. clamping plate; 411. placement plate; 412. filter screen; 413. box cover; 415. first bolt; 501. tank body; 502. first rotating shaft; 503. first motor; 504. first bevel gear; 505. second bevel gear; 506. fixing box; 507. second rotating shaft; 508. support rod; 509. blade; 510. bracket; 511. water inlet pipe; 512. tank cover. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] Example: Figure 1-4 As shown, the utility model provides a protein filtration device for molecular experiments, including a bottom plate 1, a pump body 2 is fixedly connected to the top of the bottom plate 1, a box body 6 is fixedly connected to the inner wall of the box body 6, three filter plates 7 distributed at equal intervals are fixedly connected, the outer surface of the box body 6 is connected to the discharge port 8, and the outer surface of the discharge port 8 is rotatably connected to the valve 9, the top of the bottom plate 1 is fixedly connected to the filter assembly 4, and the top side of the bottom plate 1 is fixedly connected to the crushing device 5, the crushing device 5 includes a tank body 501, the bottom end of the tank body 501 is connected to the bottom plate 1 The top side is fixedly connected, the output end of the pump body 2 is fixedly connected to the second connecting pipe 3, one end of the second connecting pipe 3 is fixedly connected to the outer surface of the tank body 501, the inner wall of the tank body 501 is rotatably connected to the first rotating shaft 502, the two ends of the first rotating shaft 502 are respectively rotatably connected to the inner walls of both sides of the tank body 501, the outer surface of the tank body 501 is fixedly connected to the first motor 503, the output end of the first motor 503 passes through the outer surface of the tank body 501 and is fixedly connected to one end of the first rotating shaft 502, and one end of the first rotating shaft 502 is fixedly connected to the first Bevel gear 504, the outer surface of the first bevel gear 504 is meshed with two second bevel gears 505, one end of the two second bevel gears 505 is fixedly sleeved with a second rotating shaft 507, the first bevel gear 504 rotates to drive the two second bevel gears 505 to rotate, and the two second bevel gears 505 rotate to drive the second rotating shaft 507 to rotate respectively, the outer surface of the first rotating shaft 502 and the two second rotating shafts 507 move together and pass through a fixed box 506, both ends of the fixed box 506 are fixedly connected to support rods 508, and the two support rods 508 are fixedly sleeved with a second rotating shaft 507. One end is fixedly connected to the inner wall of one side of the tank body 501, and the outer surfaces of the two second rotating shafts 507 respectively pass through the upper and lower ends of the fixing box 506 and are fixedly connected to blades 509. The inner wall of the tank body 501 is fixedly connected to a bracket 510, wherein the top end of the upper second rotating shaft 507 is rotatably connected to the bottom end of the bracket 510, wherein the bottom end of the lower second rotating shaft 507 is rotatably connected to the lower inner wall of the tank body 501, and two water inlet pipes 511 are fixedly connected and passed through the upper part of the outer surface of the tank body 501, and the top end of the tank body 501 is provided with a tank cover 512;

[0021] The two second bevel gears 505 rotate to drive the second rotating shaft 507 to rotate respectively, and the rotation of the two second rotating shafts 507 drives the blades 509 to rotate, which can crush the raw material and mix it with water to obtain protein raw material.

[0022] The filter assembly 4 includes a box body 401, and the two ends of the box body 401, the input end of the pump body 2, and one end of the box body 6 are fixedly penetrated by a first connecting pipe 402, and the outer surfaces of the four second connecting pipes 3 are sleeved with a sealing ring 403, one end of one of the sealing rings 403 is provided with a sealing groove 408, and one end of another sealing ring 403 is fixedly connected to a sealing ring 409 used in conjunction with the sealing groove 408, and the outer surface of the sealing ring 409 is in contact with the inner wall of the sealing groove 408. The outer surfaces of the four sealing rings 403 are fixedly connected to a fixing plate 404, and one end of the opposite surface of each two adjacent fixing plates 404 is commonly provided with a sealing gasket 405, and the two ends of the sealing gasket 405 are respectively in contact with one end of the opposite surface of the two fixing plates 404. Each of the two adjacent fixing plates 404 and one end of the sealing gasket 405 is connected through four stud bolts 406 distributed in a circular array. The outer surfaces of the four stud bolts 406 are threadedly connected to two nuts 407. The inner wall of the box body 401 is fixedly connected to a plurality of clamping plates 410. The inner wall of the box body 401 is provided with a plurality of placement plates 411 distributed at equal intervals. The inner walls of the plurality of placement plates 411 are fixedly connected to filter screens 412. After the protein is filtered through the layers of the plurality of filter screens 412, a pure amino acid solution can be obtained after the protein is filtered. The top of the box body 401 is connected to a box cover 413 by a thread. The top of the box cover 413 is threadedly inserted with four first bolts 415 distributed in a rectangular array.

[0023] Four stud bolts 406 are respectively inserted between the sealing gasket 405 and the two fixing plates 404, and nuts 407 are respectively installed on both ends of the stud bolts 406, so that the filter assembly 4 can be effectively and quickly disassembled, cleaned and installed.

[0024] Working principle: first open the tank cover 512 and pour the raw materials into the tank body 501, and pour water into the tank body 501 from the two water inlet pipes 511. When the raw materials need to be crushed, turn on the first motor 503 in the crushing device 5, and the output end of the first motor 503 rotates to drive the first rotating shaft 502 to rotate, and the rotation of the first rotating shaft 502 drives the first bevel gear 504 to rotate, and the rotation of the first bevel gear 504 drives the two second bevel gears 505 to rotate, and the rotation of the two second bevel gears 505 respectively drives the second rotating shaft 507 to rotate, and the rotation of the two second rotating shafts 507 drives the blades 509 to rotate, thereby achieving the purpose of effectively crushing the raw materials and mixing them with water to obtain protein raw materials;

[0025] The pump body 2 is turned on, and the protein inside the crushing device 5 enters one of the first connecting tubes 402 through the second connecting tube 3 through the pump body 2 and then enters the box body 401. After being filtered through multiple layers of filter screens 412, a pure amino acid solution can be obtained after the protein is filtered. When it is necessary to install the filter component 4, one end of the two sealing rings 403 is docked respectively, and the sealing gasket 405 is placed between the two fixing plates 404. The sealing ring 409 enters the inner wall of the sealing groove 408 and cooperates with the sealing gasket 405 to prevent water leakage. Four stud bolts 406 are respectively inserted between the sealing gasket 405 and the two fixing plates 404, and nuts 407 are respectively installed on both ends of the stud bolts 406, thereby achieving the purpose of effectively and quickly disassembling the filter component 4 for cleaning and installation;

[0026] The amino acid solution can be filtered again through three filter plates 7 in the box 6. The valve 9 is opened, and the pure amino acid solution can be obtained after protein filtration and can flow out from the discharge port 8.

[0027] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A protein filtration device for molecular experiments, comprising a bottom plate (1), characterized in that: The top of the bottom plate (1) is fixedly connected to a pump body (2), the top of the bottom plate (1) is fixedly connected to a box body (6), the top of the bottom plate (1) is fixedly connected to a filter assembly (4), and one side of the top of the bottom plate (1) is fixedly connected to a crushing device (5); The crushing device (5) comprises a tank body (501), the bottom end of the tank body (501) is fixedly connected to one side of the top end of the bottom plate (1), the output end of the pump body (2) is fixedly connected to a second connecting pipe (3), one end of the second connecting pipe (3) is fixedly connected to and penetrates the outer surface of the tank body (501), the inner wall of the tank body (501) is rotatably connected to a first rotating shaft (502), the two ends of the first rotating shaft (502) are respectively rotatably connected to the inner walls of both sides of the tank body (501), the outer surface of the tank body (501) is fixedly connected to a first motor (503), the output end of the first motor (503) penetrates the outer surface of the tank body (501) and is fixedly connected to one end of the first rotating shaft (502), and one end of the first rotating shaft (502) is fixedly connected to a first bevel gear. The outer surface of the first bevel gear (504) is meshedly connected to two second bevel gears (505), one end of each of the two second bevel gears (505) is fixedly sleeved with a second rotating shaft (507), the outer surfaces of the first rotating shaft (502) and the two second rotating shafts (507) are movably connected together and penetrate a fixed box (506), the outer surfaces of the two second rotating shafts (507) respectively penetrate the upper and lower ends of the fixed box (506) and are fixedly connected to blades (509), the inner wall of the tank body (501) is fixedly connected to a bracket (510), wherein the top end of the upper second rotating shaft (507) is rotatably connected to the bottom end of the bracket (510), and the bottom end of the lower second rotating shaft (507) is rotatably connected to the lower inner wall of the tank body (501).

2. A protein filtration device for molecular experiments according to claim 1, characterized in that: The filter assembly (4) comprises a box body (401), and first connecting pipes (402) are fixedly passed through both ends of the box body (401), the input end of the pump body (2), and one end of the box body (6). The outer surfaces of the four second connecting pipes (3) are sleeved with sealing rings (403), and the outer surfaces of the four sealing rings (403) are fixedly connected to fixed plates (404). One end of the opposite surface of each two adjacent fixed plates (404) is provided with a sealing gasket (405). The fixed plate (404) and one end of the sealing gasket (405) are connected through four stud bolts (406) distributed in a circular array, and the outer surfaces of the four stud bolts (406) are threadedly connected to two nuts (407). The inner wall of the box body (401) is fixedly connected to a plurality of clamping plates (410), and the inner wall of the box body (401) is provided with a plurality of placement plates (411) distributed at equal intervals, and the inner walls of the plurality of placement plates (411) are fixedly connected to filter screens (412).

3. The protein filtration device for molecular experiments according to claim 1, wherein: Both ends of the fixing box (506) are fixedly connected to support rods (508), and one end of the two support rods (508) is respectively fixedly connected to the inner wall of one side of the tank body (501).

4. A protein filtration device for molecular experiments according to claim 1, characterized in that: Two water inlet pipes (511) are fixedly connected and penetrate the upper portion of the outer surface of the tank body (501), and a tank cover (512) is provided at the top end of the tank body (501).

5. The protein filtration device for molecular experiments according to claim 1, characterized in that: The inner wall of the box body (6) is fixedly connected to three filter plates (7) distributed at equal intervals. The outer surface of the box body (6) is connected to the discharge port (8) through, and the outer surface of the discharge port (8) is rotatably connected to a valve (9).

6. A protein filtration device for molecular experiments according to claim 2, characterized in that: The two ends of the sealing gasket (405) are respectively fitted with one end of the opposite surface of the two fixing plates (404).

7. A protein filtration device for molecular experiments according to claim 2, characterized in that: One end of one of the sealing rings (403) is provided with a sealing groove (408), and one end of the other sealing ring (403) is fixedly connected with a sealing ring (409) used in conjunction with the sealing groove (408), and the outer surface of the sealing ring (409) is in contact with the inner wall of the sealing groove (408).

8. The protein filtration device for molecular experiments according to claim 2, characterized in that: The top end of the box body (401) is connected to a box cover (413) via a thread, and the top end of the box cover (413) is provided with four first bolts (415) distributed in a rectangular array via a threaded insertion.

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