Cell supernatant extraction device

Abstract

The utility model discloses a cell supernatant extraction device which comprises a precipitation cylinder, an extraction cylinder is arranged at the upper end of the precipitation cylinder, a piston rod is arranged in the extraction cylinder, an extraction pipe is arranged at the lower end of the extraction cylinder, a depth adjusting structure is arranged on the outer wall of the extraction cylinder and comprises a machine box, a lead screw is arranged in the machine box, and a screw rod is arranged in the machine box. A sliding block is arranged on the outer wall of the lead screw, one end of the sliding block penetrates through the machine box to be fixedly connected with the outer wall of the extraction cylinder, placing plates are arranged on the two sides of the lower end of the bottom plate, a limiting structure is arranged in the bottom plate, and the limiting structure comprises a threaded rod arranged in the bottom plate in a threaded mode; a guide rod is installed on one side of the upper end of the fixing base. According to the cell supernatant extraction device, impurities on the lower layer of the supernatant can be prevented from entering the extraction pipe in the extraction process, so that an extraction material is not pure.

Description

Technical Field

[0001] The utility model relates to the field of cell extraction, in particular to a cell supernatant extraction device. Background Art

[0002] Cell supernatant extraction devices are typically used to extract supernatant from cell culture medium or bioreactors. These devices are widely used in biopharmaceuticals, bioengineering, cell culture, and other fields to collect cell culture medium, proteins, cytokines, and other substances dissolved in the culture medium.

[0003] The existing extraction tube requires the operator to hold it and place the extraction tube into the cell supernatant to extract the supernatant. However, long-term holding and shaking may cause the lower end of the extraction tube to be unstable, resulting in the direct extraction of materials in the lower layer of the supernatant, resulting in impure supernatant extraction. Utility Model Content

[0004] The main purpose of the utility model is to provide a cell supernatant extraction device, which can effectively solve the technical problems in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A cell supernatant extraction device includes a sedimentation cylinder, an extraction cylinder is provided at the upper end of the sedimentation cylinder, a piston rod is provided inside the extraction cylinder, an extraction tube is provided at the lower end of the extraction cylinder, the outer wall of the extraction cylinder is provided with a depth adjustment structure, the depth adjustment structure includes a chassis, a screw rod is provided inside the chassis, and a slider is provided on the outer wall of the screw rod. One end of the slider passes through the chassis and is fixedly connected to the outer wall of the extraction cylinder, and placement plates are provided on both sides of the lower end of the bottom plate.

[0007] As a further solution of the present invention, a limiting structure is provided inside the base plate, and the limiting structure includes a screw with threads provided inside the base plate, a fixed seat is rotatably provided at the lower end of the screw, a marking rod is provided at the lower end of the fixed seat, and a guide rod is installed on one side of the upper end of the fixed seat.

[0008] As a further solution of the present invention, a contact plate is installed at the lower end of the guide rod.

[0009] As a further solution of the present invention, a telescopic rod is movably provided on one side of the placement plate, a reinforcement cover is fixedly provided on the lower end of the telescopic rod, and a groove is provided at the lower end of the reinforcement cover.

[0010] As a further solution of the present invention, the groove is in an arc shape.

[0011] As a further solution of the present invention, a knob is provided at the rear end of the placement plate, and an output end of the knob passes through the rear end of the placement plate and contacts the rear end of the telescopic rod.

[0012] As a further solution of the present invention, the upper end of the extraction tube is threadedly connected to the lower end of the extraction cylinder.

[0013] The beneficial effects of the utility model are as follows:

[0014] By setting up a depth adjustment structure, the extraction tube can be placed on the upper end of the sedimentation tube, and the position of the lower end of the extraction tube can be manually adjusted according to the thickness of the supernatant, so as to avoid the operator shaking the hand, causing the extraction tube to be inserted into the lower end of the supernatant and causing impure extraction;

[0015] By setting a limiting structure, before adjusting the depth of the lower end of the extraction tube, the depth of the supernatant is detected by adjusting the position of the marking rod. When the contact plate contacts the lower layer of the supernatant, it stops. When the extraction tube is lowered, the extraction tube is limited to prevent the extraction tube from being inserted into the lower end of the supernatant. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of a cell supernatant extraction device of the present invention;

[0017] Figure 2 This is a structural diagram of a telescopic rod of a cell supernatant extraction device of the present utility model;

[0018] Figure 3 This is a cross-sectional structural diagram of a sedimentation cylinder of a cell supernatant extraction device of the present invention;

[0019] Figure 4 This is a structural diagram of a marker rod of a cell supernatant extraction device of the present invention;

[0020] Figure 5 for Figure 3 Enlarged structural diagram at point A in the middle.

[0021] In the figure: 1. Sedimentation cylinder; 2. Extraction cylinder; 3. Piston rod; 4. Extraction tube; 5. Placement plate; 6. Bottom plate; 7. Depth adjustment structure; 8. Chassis; 9. Screw; 10. Slider; 11. Limiting structure; 12. Screw; 13. Fixed seat; 14. Marking rod; 15. Guide rod; 16. Contact plate; 17. Telescopic rod; 18. Reinforcement cover; 19. Knob. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] like Figure 1-5 As shown, a cell supernatant extraction device includes a sedimentation cylinder 1, an extraction cylinder 2 is provided at the upper end of the sedimentation cylinder 1, a piston rod 3 is provided inside the extraction cylinder 2, an extraction tube 4 is provided at the lower end of the extraction cylinder 2, and a depth adjustment structure 7 is provided on the outer wall of the extraction cylinder 2. The depth adjustment structure 7 includes a chassis 8, a screw rod 9 is provided inside the chassis 8, and a slider 10 is provided on the outer wall of the screw rod 9. One end of the slider 10 passes through the chassis 8 and is fixedly connected to the outer wall of the extraction cylinder 2. Placement plates 5 are provided on both sides of the lower end of the bottom plate 6.

[0024] In this embodiment, a limiting structure 11 is provided inside the base plate 6. The limiting structure 11 includes a screw 12 with internal threads provided on the base plate 6. A fixed seat 13 is rotatably provided at the lower end of the screw 12. A marking rod 14 is provided at the lower end of the fixed seat 13. A guide rod 15 is installed on one side of the upper end of the fixed seat 13.

[0025] The screw 12 is used to adjust the position of the fixed seat 13, and the fixed seat 13 is used to install the marking rod 14. The marking rod 14 is used to contact the material at the lower end of the supernatant and limit the lower end of the extraction tube 4. The guide rod 15 ensures that the fixed seat 13 moves steadily.

[0026] In this embodiment, a contact plate 16 is mounted on the lower end of the guide rod 15 .

[0027] The contact plate 16 enters the lower layer of the supernatant to limit the position of the extraction tube 4.

[0028] In this embodiment, a telescopic rod 17 is movably provided on one side of the placement plate 5 , a reinforcement cover 18 is fixedly provided at the lower end of the telescopic rod 17 , and a groove is provided at the lower end of the reinforcement cover 18 .

[0029] The telescopic rod 17 is used to set the reinforcement cover 18, and the reinforcement cover 18 is used to be clamped on the outer wall of the upper end of the sedimentation cylinder 1.

[0030] In this embodiment, the groove is in an arc shape.

[0031] The arc shape can ensure that the reinforcement cover 18 is firmly connected.

[0032] In this embodiment, a knob 19 is provided at the rear end of the placement plate 5 , and an output end of the knob 19 passes through the rear end of the placement plate 5 and contacts the rear end of the telescopic rod 17 .

[0033] The knob 19 is used to tighten the telescopic rod 17 to ensure the stability of the telescopic rod 17.

[0034] In this embodiment, the upper end of the extraction tube 4 is threadedly connected to the lower end of the extraction cylinder 2 .

[0035] The threaded connection can facilitate the removal of the extraction tube 4 at a later time.

[0036] It should be noted that the present invention is a cell supernatant extraction device. When in use, the telescopic rod 17 is pulled to clamp the reinforcement cover 18 on the outer wall of the upper end of the sedimentation cylinder 1, and the knob 19 is turned so that the front end of the knob 19 is squeezed to the rear end of the telescopic rod 17. The screw 12 is rotated so that the screw 12 drives the fixing seat 13 to move downward, thereby causing the marking rod 14 to move downward to the upper end of the supernatant to achieve position limiting.

[0037] By rotating the screw rod 9, the slider 10 drives the extraction cylinder 2 to move downward. When the lower end of the extraction tube 4 contacts the upper end of the contact plate 16, it will not move downward any further. The extraction tube 4 is limited and the piston rod 3 is slowly pulled to extract the supernatant into the extraction cylinder 2. The extraction of the supernatant is achieved. During the whole process, there is no need to worry about the extraction tube 4 entering the lower end of the supernatant and causing the extraction of other substances.

[0038] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A cell supernatant extraction device, comprising a sedimentation cylinder (1), an extraction cylinder (2) provided at the upper end of the sedimentation cylinder (1), a piston rod (3) provided inside the extraction cylinder (2), and an extraction tube (4) provided at the lower end of the extraction cylinder (2), characterized in that: The outer wall of the extraction cylinder (2) is provided with a depth adjustment structure (7), the depth adjustment structure (7) includes a chassis (8), a screw rod (9) is provided inside the chassis (8), a slider (10) is provided on the outer wall of the screw rod (9), one end of the slider (10) passes through the chassis (8) and is fixedly connected to the outer wall of the extraction cylinder (2), and placement plates (5) are provided on both sides of the lower end of the bottom plate (6).

2. A cell supernatant extraction device according to claim 1, characterized in that: A limiting structure (11) is provided inside the base plate (6), and the limiting structure (11) includes a screw (12) provided with a thread inside the base plate (6), a fixing seat (13) is rotatably provided at the lower end of the screw (12), a marking rod (14) is provided at the lower end of the fixing seat (13), and a guide rod (15) is installed on one side of the upper end of the fixing seat (13).

3. The cell supernatant extraction device according to claim 2, characterized in that: A contact plate (16) is installed at the lower end of the guide rod (15).

4. The cell supernatant extraction device according to claim 1, characterized in that: A telescopic rod (17) is movably provided on one side of the placement plate (5), a reinforcement cover (18) is fixedly provided at the lower end of the telescopic rod (17), and a groove is provided at the lower end of the reinforcement cover (18).

5. The cell supernatant extraction device according to claim 4, characterized in that: The groove is in an arc shape.

6. The cell supernatant extraction device according to claim 4, characterized in that: The rear end of the placement plate (5) is provided with a knob (19), and the output end of the knob (19) passes through the rear end of the placement plate (5) and contacts the rear end of the telescopic rod (17).

7. The cell supernatant extraction device according to claim 1, characterized in that: The upper end of the extraction tube (4) is threadedly connected to the lower end of the extraction cylinder (2).

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