Automatic pretreatment device for aquatic organism eDNA sample

By designing an automatic pretreatment device for aquatic biological eDNA samples and using a combination structure of multiple filter cartridges and gears, multiple samples are simultaneous screening and cleaning, solving the problem of low pretreatment efficiency of water samples and improving the degree of automation and efficiency.

CN223255255UActive Publication Date: 2025-08-22NANJING BOSCO ENVIRONMENTAL TECH CO LTD
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Patent Information

Application Number
CN202422425994.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-10-09
Publication Date
2025-08-22
Estimated Expiration
2034-10-09

AI Technical Summary

Technical Problem

In the prior art, water sample filtration pretreatment requires manual operation, which increases the labor intensity of personnel and is inefficient, so that multiple samples cannot be processed simultaneously.

Method used

An automatic pretreatment device for aquatic biological eDNA samples is designed, and a combination structure of pump body, circular slide chute, slider, support plate, pump body, suction tube, discharge tube, driving gear, driven gear and filter cartridge is used to realize the simultaneous extraction and screening of multiple samples, and the motor drive gear drives multiple filter cartridges to rotate for screening.

Benefits of technology

It improves the efficiency of water sample pretreatment, can process multiple samples at the same time, and cleans the filter cartridge through reverse flushing, reducing manual labor intensity.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to an automatic pretreatment device for an aquatic organism eDNA (Deoxyribose Nucleic Acid) sample, and aims to solve the technical problems that the labor intensity of personnel is increased and the water sample pretreatment efficiency is influenced because the personnel need to manually operate when a collected water sample is subjected to filtering pretreatment at present, and only one water sample can be pretreated by the personnel at one time. Comprising a cylinder body, a driving gear is rotatably connected to the middle of the bottom end of an inner cavity of the cylinder body through a rotating shaft, four connecting shafts are rotatably connected to the bottom end of the inner cavity of the cylinder body, driven gears are fixedly connected to the outer sides of the four connecting shafts, the four driven gears are engaged with the driving gear, and filter cylinders are arranged at the tops of the four connecting shafts; the bottom of the cylinder body is communicated with an L-shaped drainage pipe; a round sliding groove is formed in the outer side of the cylinder body, a sliding block is connected into the round sliding groove in a sliding mode, samples in the four cylinders can be screened at the same time, and then the water sample pretreatment efficiency is greatly improved.
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Description

Technical Field

[0001] The utility model relates to the field of aquatic organism pretreatment, in particular to an automatic pretreatment device for aquatic organism eDNA samples. Background Art

[0002] When conducting ecological health diagnostics on water bodies such as rivers, lakes, and oceans, it is necessary to sample aquatic plankton to understand their diversity. Currently, plankton sampling in aquatic ecosystems is primarily done by manually collecting water samples on-site multiple times a year. These samples are then brought back to the laboratory for enrichment and subsequent purification, extraction, and analysis.

[0003] Traditionally, when filtering and pre-treating collected water samples, manual operation is required, which not only increases the labor intensity of the personnel, but also the personnel can only pre-treat one water sample at a time, thus affecting the efficiency of water sample pre-treatment. Therefore, a new technical solution needs to be designed to solve this problem. Utility Model Content

[0004] The purpose of this utility model is to overcome the shortcomings of the existing technology, adapt to actual needs, and provide an automatic pretreatment device for aquatic organism eDNA samples to solve the technical problem that when filtering and pretreating the collected water samples, manual operation is required, which not only increases the labor intensity of the personnel, but also the personnel can only pretreat one water sample at a time, thereby affecting the efficiency of water sample pretreatment.

[0005] In order to achieve the purpose of the utility model, the technical solution adopted by the utility model is as follows: an automatic pretreatment device for eDNA samples of aquatic organisms is designed, comprising a cylinder, wherein the middle portion of the bottom end of the inner cavity of the cylinder is rotatably connected to a driving gear via a rotating shaft, the bottom end of the inner cavity of the cylinder is rotatably connected to four connecting shafts, the outer sides of the four connecting shafts are fixedly connected to driven gears, the four driven gears are meshed with the driving gear, the tops of the four connecting shafts are provided with filter cartridges, and the bottom of the cylinder is connected to an L-shaped drain pipe;

[0006] A circular chute is provided on the outer side of the cylinder, a slider is slidably connected in the circular chute, a support plate is connected to the slider, a pump body is installed on the support plate, the suction end of the pump body is connected to a suction pipe, and the discharge end of the pump body is connected to a discharge pipe.

[0007] Preferably, a hexagonal column is fixedly connected to the bottom of the filter cartridge, a hexagonal notch is opened on the top of the connecting shaft, and the hexagonal column is adapted to the hexagonal notch.

[0008] Preferably, four water inlet pipes are passed through the bottom end of the inner cavity of the cylinder, and water outlet holes are formed at one end of the four water inlet pipes close to the filter cartridge.

[0009] Preferably, support legs are installed at the four corners of the bottom of the cylinder, a bottom plate is fixedly connected between the bottoms of the four support legs, and universal wheels are installed at the four corners of the bottom of the four bottom plates, and the universal wheels have a self-locking function.

[0010] Preferably, both upper and lower ends of the inner cavity of the circular slide groove are provided with limiting grooves, both limiting blocks are slidably connected in the two limiting grooves, and both limiting blocks are connected by sliders.

[0011] Preferably, a fixing bolt passes through one outer end of the support plate, and the fixing bolt contacts the inner wall of the circular sliding groove.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. The utility model combines a pump body, a circular chute, a slider, a support plate, a pump body, a suction pipe, a discharge pipe, a driving gear, a driven gear, a connecting shaft and a filter cartridge. First, the suction pipe is placed in water, and then the pump body is started to draw the sample into the filter cartridge. At the same time, the slider slides in the circular chute, so that different water samples can be drawn into different filter cartridges. Then, the motor drives the driving gear to rotate, and then cooperates with the driven gear to synchronously drive the four filter cartridges to rotate for screening, so that the samples of the four cartridges can be screened at the same time, thereby greatly improving the efficiency of water sample pretreatment.

[0014] 2. The utility model combines the water inlet pipe and the water outlet hole. After the filter cartridge is used up, clean water enters through the water inlet pipe, and then the four filter cartridges are driven to rotate by the motor, so that the filter cartridge can be reversely flushed, making it easier to clean the filter cartridge after use. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the top view of the structure of the cylinder body connected with the driving gear and the driven gear of the utility model;

[0017] Figure 3 This is a schematic diagram of the connection structure between the filter cartridge and the hexagonal column of the utility model;

[0018] Figure 4 It is an enlarged view of point A of the present utility model.

[0019] In the figure: 1. Cylinder; 11. L-shaped drain pipe; 12. Circular chute; 13. Slider; 14. Limiting groove; 15. Limiting block; 2. Filter cartridge; 21. Hexagonal column; 3. Water inlet pipe; 4. Support leg; 41. Bottom plate; 42. Universal wheel; 5. Support plate; 51. Fixing bolt; 6. Pump body; 61. Suction pipe; 62. Discharge pipe; 7. Driving gear; 8. Connecting shaft; 81. Hexagonal notch; 82. Driven gear. DETAILED DESCRIPTION

[0020] The present invention is further described below with reference to the accompanying drawings and embodiments:

[0021] Example 1: An automatic pre-processing device for aquatic organism eDNA samples, see Figures 1 to 4 , including a cylinder 1, the middle part of the bottom end of the inner cavity of the cylinder 1 is rotatably connected to a driving gear 7 through a rotating shaft, the bottom end of the inner cavity of the cylinder 1 is rotatably connected to four connecting shafts 8, the outer sides of the four connecting shafts 8 are fixedly connected to driven gears 82, the four driven gears 82 are engaged with the driving gear 7, the tops of the four connecting shafts 8 are provided with filter cartridges 2, and the bottom of the cylinder 1 is connected to an L-shaped drain pipe 11; a circular chute 12 is opened on the outer side of the cylinder 1, a slider 13 is slidably connected in the circular chute 12, and a support plate 5 is connected to the slider 13. A pump body 6 is installed on the plate 5. The suction end of the pump body 6 is connected to a suction pipe 61, and the discharge end of the pump body 6 is connected to a discharge pipe 62. When working, the suction pipe 61 is first placed in water, and then the pump body 6 is started to draw the sample into the filter cartridge 2. At the same time, the slider 13 slides in the circular chute 12, so that water samples of different samples can be drawn into different filter cartridges 2, and then the motor drives the driving gear 7 to rotate, and then cooperates with the driven gear 82 to synchronously drive the four filter cartridges 2 to rotate for screening, so that the samples of the four cartridges can be screened at the same time, thereby greatly improving the efficiency of water sample pretreatment.

[0022] For details, see Figure 2 and Figure 3 The bottom of the filter cartridge 2 is fixedly connected with a hexagonal column 21, and the top of the connecting shaft 8 is provided with a hexagonal slot 81. The hexagonal column 21 is adapted to the hexagonal slot 81. By inserting the hexagonal column 21 into the hexagonal slot 81, it is convenient to disassemble and assemble the filter cartridge 2 and the connecting shaft 8, and then it is convenient to take out the screened filter cartridge 2 and extract the screened sample.

[0023] For further information, see Figure 2Four water inlet pipes 3 are passed through the bottom end of the inner cavity of the cylinder 1. The four water inlet pipes 3 are each provided with a water outlet hole at one end close to the filter cartridge 2. When the filter cartridge 2 is used up, clean water enters through the water inlet pipe 3, and then the four filter cartridges 2 are driven to rotate by the motor, so that the filter cartridge 2 can be reversely flushed, making it easier to clean the filter cartridge 2 after use.

[0024] It is worth noting that, see Figure 1 Support legs 4 are installed at the four corners of the bottom of the cylinder 1, and a bottom plate 41 is fixedly connected between the bottoms of the four support legs 4. Universal wheels 42 are installed at the four corners of the bottom of the four bottom plates 41, and the universal wheels 42 have a self-locking function. The universal wheels 42 are used to facilitate moving the device to the working area for use, and the universal wheels 42 can self-lock during use to improve the stability of the device when in use.

[0025] It is worth noting that see Figure 1 and Figure 4 , the upper and lower ends of the inner cavity of the circular slide 12 are provided with limiting grooves 14, and the two limiting blocks 15 are slidably connected in the two limiting grooves 14, and the two limiting blocks 15 are connected to the slider 13. A fixing bolt 51 is passed through the outer end of the support plate 5, and the fixing bolt 51 contacts the inner wall of the circular slide 12. The limiting block 15 guides the movement in the limiting groove 14, thereby improving the stability of the slider 13 in the circular slide 12, thereby improving the stability of the pump body 6, and when the pump body 6 is in use, by twisting the fixing bolt 51 to contact the inner wall of the circular slide 12, it is convenient to limit the pump body 6, thereby improving the stability of the pump body 6 when in use.

[0026] In addition, the components designed in this utility model are all universal standard parts or components known to technical personnel in this field. Their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods. They can be fully implemented by technical personnel in this field. Needless to say, the content protected by this utility model does not involve improvements to internal structures and methods.

[0027] The embodiments disclosed in the present invention are preferred embodiments, but are not limited to them. Ordinary technicians in this field can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. As long as they do not deviate from the spirit of the present invention, they are all within the scope of protection of the present invention.

Claims

1. An automatic pretreatment device for eDNA samples of aquatic organisms, comprising a cylinder (1), characterized in that: The middle portion of the bottom end of the inner cavity of the cylinder (1) is rotatably connected to a driving gear (7) via a rotating shaft, and the bottom end of the inner cavity of the cylinder (1) is rotatably connected to four connecting shafts (8), and the outer sides of the four connecting shafts (8) are fixedly connected to driven gears (82), and the four driven gears (82) are all meshed with the driving gear (7). The tops of the four connecting shafts (8) are all provided with filter cartridges (2), and the bottom of the cylinder (1) is connected to an L-shaped drain pipe (11); A circular chute (12) is provided on the outer side of the cylinder (1), a slider (13) is slidably connected in the circular chute (12), a support plate (5) is connected to the slider (13), a pump body (6) is mounted on the support plate (5), a suction end of the pump body (6) is connected to a suction pipe (61), and a discharge end of the pump body (6) is connected to a discharge pipe (62).

2. The automatic pretreatment device for aquatic organism eDNA samples according to claim 1, characterized in that: A hexagonal column (21) is fixedly connected to the bottom of the filter cartridge (2), a hexagonal notch (81) is provided on the top of the connecting shaft (8), and the hexagonal column (21) is adapted to the hexagonal notch (81).

3. The automatic pretreatment device for aquatic organism eDNA samples according to claim 1, characterized in that: Four water inlet pipes (3) are passed through the bottom end of the inner cavity of the cylinder (1), and water outlet holes are provided at one end of the four water inlet pipes (3) close to the filter cartridge (2).

4. The automatic pretreatment device for aquatic organism eDNA samples according to claim 1, characterized in that: Support legs (4) are installed at the four corners of the bottom of the cylinder (1), a bottom plate (41) is fixedly connected between the bottoms of the four support legs (4), and universal wheels (42) are installed at the four corners of the bottom of the four bottom plates (41), and the universal wheels (42) have a self-locking function.

5. The automatic pre-processing device for aquatic organism eDNA samples according to claim 1, characterized in that: Limiting grooves (14) are provided at both upper and lower ends of the inner cavity of the circular slide groove (12), and limiting blocks (15) are slidably connected in the two limiting grooves (14), and the two limiting blocks (15) are connected to the slider (13).

6. The automatic pre-processing device for aquatic organism eDNA samples according to claim 1, characterized in that: A fixing bolt (51) passes through one end of the outer side of the support plate (5), and the fixing bolt (51) contacts the inner wall of the circular sliding groove (12).