Fluorescent isothermal amplification device

Abstract

The utility model provides a fluorescent isothermal amplification device, which comprises a shell, a fluorescent lamp tube, a fluorescent lamp tube and a fluorescent lamp tube, the heat cover assembly comprises an outer cover, an upper heating plate, a heat plate mounting bracket and a bottom cover, and the heat plate mounting bracket is mounted in the outer cover in a manner of moving up and down through a spring; and the temperature control detection unit comprises a test tube seat, a lower heating plate, an exciting light source, an exciting light filter, an emission light filter and a fluorescence detector. According to the utility model, the touch display screen for setting and adjusting parameters is arranged on the upper surface of the shell and occupies most of the area, so that the operation is convenient; the heat cover assembly occupies a small space in the front of the shell and is suitable for detection of a small number of samples; the structure is compact and small, carrying is convenient, and on-site detection requirements are met.

Description

Technical Field

[0001] The utility model relates to the technical field of molecular diagnosis, in particular to a fluorescence isothermal amplification device. Background Art

[0002] A fluorescence isothermal amplification device is a device that combines isothermal amplification technology with fluorescence detection technology, used to achieve rapid amplification and real-time monitoring of nucleic acids at a constant temperature. Nucleic acid isothermal amplification technology is a method for amplifying DNA or RNA at a constant temperature. This method avoids the complex process of DNA denaturation, primer annealing, and DNA extension required in traditional PCR technology, which requires constant temperature changes. Fluorescence signal detection technology monitors the progress of a reaction in real time by detecting changes in fluorescence intensity within the reaction system. In isothermal amplification fluorescence detection, one or more fluorescent dyes or fluorescently labeled probes are typically added to the reaction system. These fluorescent dyes or probes can bind to the amplified DNA or RNA double strands, emitting a fluorescent signal.

[0003] Current fluorescence isothermal amplification devices are mostly configured based on batch testing, with relatively complex structures and large volumes, making them not compact enough for portability. Therefore, a fluorescence isothermal amplification device solution that is easy to operate and compact enough for portability is needed. Utility Model Content

[0004] In order to overcome the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a fluorescence isothermal amplification device.

[0005] To achieve the above-mentioned purpose, the present invention solves the technical problem by adopting a technical solution: a fluorescence isothermal amplification device, comprising:

[0006] a housing having a touch display screen mounted on its upper surface;

[0007] a thermal cover assembly located in front of the touch screen display on the housing, the thermal cover assembly being openably and closably mounted on the housing, the thermal cover assembly comprising an outer cover, an upper heating plate, a hot plate mounting bracket, and a bottom cover, the hot plate mounting bracket being mounted in the outer cover so as to be movable up and down via a spring, the upper heating plate being mounted on the hot plate mounting bracket, the bottom cover being mounted on the bottom of the outer cover, and the bottom cover being provided with an opening for the test tube head to pass through;

[0008] A temperature control and detection unit is located at the front part of the shell, and the temperature control and detection unit is located directly below the hot cover assembly. The temperature control and detection unit includes a test tube holder, a lower heating plate, an excitation light source, an excitation filter, an emission filter and a fluorescence detector. The test tube holder is provided with a plurality of holes and slots adapted to the outer wall of the test tube, and an excitation light transmission hole is provided at the bottom of each hole and slot of the test tube holder. The excitation filter is installed at the position of the excitation light transmission hole, the excitation light source is located below the excitation filter, and an emission light transmission hole is provided on one side of the lower part of each hole and slot of the test tube holder. The emission filter is installed at the position of the emission light transmission hole, and the fluorescence detector is located on the side of the emission filter. The lower heating plate is attached to the side of the test tube holder opposite to the emission filter.

[0009] By adopting the technical solution of the utility model, the touch display screen for parameter setting and adjustment is arranged on the upper surface of the shell and occupies most of the area, which is convenient for operation; the thermal cover assembly occupies a small space in the front of the shell, which is suitable for the detection of a small number of samples; the structure is compact and small, easy to carry, and can meet the needs of on-site detection.

[0010] Furthermore, the excitation light source is an LED light source.

[0011] Furthermore, the excitation filter is a blue excitation filter or a green excitation filter.

[0012] Furthermore, the emission filter is a yellow emission filter, an orange emission filter or a red emission filter.

[0013] Furthermore, the upper heating plate and the lower heating plate are aluminum substrates, and the aluminum substrates are provided with heating circuits with a zigzag distribution.

[0014] The above preferred solution is adopted to improve heating uniformity.

[0015] Furthermore, a locking member for controlling the opening and closing of the thermal cover assembly is provided on the shell.

[0016] The above preferred solution is adopted to facilitate the opening and closing of the thermal cover assembly.

[0017] Furthermore, the test tube holder is provided with 16 slots for placing two groups of eight-tubes, and the 16 slots are in the same straight line.

[0018] By adopting the above-mentioned preferred solution, the space at the front edge of the shell is fully utilized, and the detection capability is maximized while maintaining a compact structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 It is a structural diagram of an implementation method of the utility model.

[0021] Figure 2 It is a cross-sectional view of an embodiment of the present invention.

[0022] Figure 3 yes Figure 2 A partial enlarged view of point A in the middle.

[0023] Figure 4 It is a structural schematic diagram of a hidden thermal cover assembly in one embodiment of the present invention.

[0024] The numbers and letters in the figure represent the names of the corresponding parts:

[0025] 10-housing; 11-locking part; 20-touch screen; 30-heat cover assembly; 31-outer cover; 32-upper heating plate; 33-hot plate mounting bracket; 34-bottom cover; 40-temperature control detection unit; 41-test tube holder; 411-hole slot; 42-lower heating plate; 43-excitation light source; 44-excitation filter; 45-emission filter; 46-fluorescence detector; 47-excitation light transmission hole; 48-emission light transmission hole. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0027] like Figure 1 As shown, a fluorescence isothermal amplification device comprises:

[0028] The housing 10 has a touch screen 20 mounted on its upper surface;

[0029] The thermal cover assembly 30 is located in front of the touch screen 20 on the housing 10 and is installed on the housing 10 in an openable and closable manner. The thermal cover assembly 30 includes an outer cover 31, an upper heating plate 32, a heating plate mounting bracket 33, and a bottom cover 34. The heating plate mounting bracket 33 is mounted in the outer cover 31 so as to be movable up and down via a spring. The upper heating plate 32 is mounted on the heating plate mounting bracket 33. The bottom cover 34 is mounted on the bottom of the outer cover 31 and is provided with an opening for the test tube head to pass through.

[0030] The temperature control and detection unit 40 is located at the front part of the shell 10 and is directly below the heat cover assembly 30. The temperature control and detection unit 40 includes a test tube holder 41, a lower heating plate 42, an excitation light source 43, an excitation filter 44, an emission filter 45 and a fluorescence detector 46. The test tube holder 41 is provided with a plurality of holes 411 adapted to the outer wall of the test tube. An excitation light transmission hole 47 is provided at the bottom of each hole of the test tube holder 41. The excitation filter 44 is installed at the position of the excitation light transmission hole 47. The excitation light source 43 is located below the excitation filter 44. An emission light transmission hole 48 is provided on one side of the lower part of each hole of the test tube holder 41. The emission filter 45 is installed at the position of the emission light transmission hole 48. The fluorescence detector 46 is located on the side of the emission filter 45. The lower heating plate 42 is attached to the side of the test tube holder 41 opposite to the emission filter 45.

[0031] During testing, the temperature generated by the heating plate on the heated cover assembly is higher than the temperature of the downward-facing heating plate on the test tube holder side of the temperature-controlled detection unit to prevent upward evaporation of the sample solution. The excitation light emitted by the excitation light source is filtered by the excitation filter and then irradiated onto the sample in the test tube on the test tube holder. The fluorescent dye in the sample is excited and produces a fluorescence signal. The emission filter blocks the excitation light and allows the fluorescence to pass through. The fluorescence detector detects the fluorescence signal and quantitatively determines the content of biomolecules such as proteins and nucleic acids.

[0032] The beneficial effects of adopting the above technical solution are: the touch display screen for parameter setting and adjustment is arranged on the upper surface of the shell and occupies most of the area, which is convenient for operation; the thermal cover assembly occupies a small space in the front of the shell, which is suitable for testing a small number of samples; the structure is compact and small, easy to carry, to meet the needs of on-site testing.

[0033] In other embodiments of the present invention, the excitation light source 43 is LED light.

[0034] In other embodiments of the present invention, the excitation filter 44 is a blue excitation filter or a green excitation filter.

[0035] In other embodiments of the present invention, the emission filter 45 is a yellow emission filter, an orange emission filter, or a red emission filter.

[0036] In some other embodiments of the present invention, the upper heating plate 32 and the lower heating plate 42 are aluminum substrates, and the aluminum substrates are provided with zigzag heating circuits. The beneficial effect of adopting the above technical solution is: improving heating uniformity.

[0037] In some other embodiments of the present invention, a locking member 11 is provided on the housing 10 for controlling the opening and closing of the thermal cover assembly, thereby facilitating the opening and closing of the thermal cover assembly.

[0038] In other embodiments of the present invention, the test tube holder 41 is provided with 16 slots 411 for accommodating two sets of eight-tube strips, with the 16 slots 411 being aligned in a straight line. The above technical solution has the beneficial effect of fully utilizing the front edge space of the housing, maximizing detection capabilities while maintaining a compact structure.

[0039] The above embodiments are only for illustrating the technical concept and features of the present invention. Their purpose is to enable ordinary technicians in this field to understand the content of the present invention and implement it. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of protection of the present invention.

Claims

1. A fluorescence isothermal amplification device, characterized in that: include: a housing, the upper surface of which is mounted with a touch display screen; a thermal cover assembly located in front of the touch screen display on the housing, the thermal cover assembly being openably and closably mounted on the housing, the thermal cover assembly comprising an outer cover, an upper heating plate, a hot plate mounting bracket, and a bottom cover, the hot plate mounting bracket being mounted in the outer cover so as to be movable up and down via a spring, the upper heating plate being mounted on the hot plate mounting bracket, the bottom cover being mounted on the bottom of the outer cover, and the bottom cover being provided with an opening for the test tube head to pass through; A temperature control and detection unit is located at the front part of the shell, and the temperature control and detection unit is located directly below the hot cover assembly. The temperature control and detection unit includes a test tube holder, a lower heating plate, an excitation light source, an excitation filter, an emission filter and a fluorescence detector. The test tube holder is provided with a plurality of holes and slots adapted to the outer wall of the test tube, and an excitation light transmission hole is provided at the bottom of each hole and slot of the test tube holder. The excitation filter is installed at the position of the excitation light transmission hole, the excitation light source is located below the excitation filter, and an emission light transmission hole is provided on one side of the lower part of each hole and slot of the test tube holder. The emission filter is installed at the position of the emission light transmission hole, and the fluorescence detector is located on the side of the emission filter. The lower heating plate is attached to the side of the test tube holder opposite to the emission filter.

2. The fluorescence isothermal amplification device according to claim 1, characterized in that The excitation light source is an LED light source.

3. The fluorescence isothermal amplification device according to claim 2, characterized in that: The excitation filter is a blue excitation filter or a green excitation filter.

4. The fluorescence isothermal amplification device according to claim 3, characterized in that: The emission filter is a yellow emission filter, an orange emission filter or a red emission filter.

5. The fluorescence isothermal amplification device according to claim 1, characterized in that: The upper heating plate and the lower heating plate are aluminum substrates, and the aluminum substrates are provided with heating circuits with a zigzag distribution.

6. The fluorescence isothermal amplification device according to claim 1, characterized in that: The shell is provided with a locking member for controlling the opening and closing of the thermal cover assembly.

7. The fluorescence isothermal amplification device according to claim 1, characterized in that: The test tube holder is provided with 16 holes for placing two groups of eight-tube connections, and the 16 holes are in the same straight line.

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