Fluorescence detection module and fluorescence detection device

Abstract

The utility model discloses a fluorescence detection module and a fluorescence detection device. The fluorescence detection module comprises a light source assembly, a first dichroscope, a second dichroscope, a light filtering assembly and a photoelectric detection assembly, the first dichroscope is arranged on an emergent light path of the light source assembly and is used for reflecting light in a first direction generated by the light source assembly to a second direction so as to reach a sample to be detected; the second dichroscope is used for reflecting fluorescence generated by the to-be-detected sample to a third direction so as to reach the light filtering assembly, and the first direction and the third direction are respectively located on a plane perpendicular to the second direction; the optical filter is used for filtering the fluorescent light, and the photoelectric detection assembly is used for performing photoelectric conversion treatment on the filtered fluorescent light. The optical path in the fluorescence detection module is optimized, and the size of the fluorescence detection module in the direction from the sample to be detected to the photoelectric detection assembly is reduced, so that the size of an instrument loaded with the fluorescence detection module can be reduced, and the application range of the fluorescence detection module is expanded.

Description

Technical Field

[0001] The present application relates to the field of fluorescence detection technology, and in particular to a fluorescence detection module and a fluorescence detection device. Background Art

[0002] At present, handheld fluorescence analyzers equipped with fluorescence detection modules have been widely used due to their convenience. In addition, fluorescence detection modules are also used as core accessories in many analytical instruments.

[0003] However, the existing fluorescence detection module is equipped with more optical elements in the direction from the sample to be tested to the photoelectric detection component. Specifically, the light source and excitation filter are arranged in the horizontal direction, and the photoelectric detection component, detection filter, dichroic mirror and objective lens are arranged in the vertical direction in sequence, resulting in the overall size of the fluorescence detection module being relatively long, which has a certain impact on the application of the fluorescence detection module in many instruments.

[0004] In addition, since most of the samples to be tested are wet samples, their placement direction must be consistent with the direction of gravity. Therefore, the overall longer size of the fluorescence detection module will limit the size of the matching handheld fluorescence analyzer from being further reduced, resulting in the application scope of the fluorescence detection module being unable to be further expanded. Utility Model Content

[0005] In view of this, the present application provides a fluorescence detection module and a fluorescence detection device, aiming to solve the problems existing in the prior art described in the background technology.

[0006] To achieve the above-mentioned object, the present application provides a fluorescence detection module, comprising: a light source assembly, a first dichroic mirror, a second dichroic mirror, a filter assembly, and a photoelectric detection assembly;

[0007] A first dichroic mirror is provided on the outgoing light path of the light source assembly, and is used to reflect the light generated in a first direction by the light source assembly to a second direction so as to reach the sample to be tested;

[0008] The second dichroic mirror is used to reflect the fluorescence generated by the sample to be tested in a third direction to reach the filter assembly, wherein the first direction and the third direction are respectively located on a plane perpendicular to the second direction;

[0009] The filter component is used to filter the fluorescence, and the photoelectric detection component is used to perform photoelectric conversion processing on the filtered fluorescence.

[0010] Optionally, the first dichroic mirror and the second dichroic mirror are arranged on the fluorescence emission light path of the sample to be tested, the first dichroic mirror is closer to the sample placement side than the second dichroic mirror, and the first dichroic mirror has a transmission effect on the fluorescence generated by the sample to be tested.

[0011] Optionally, the angle between the first direction and the third direction is 90°.

[0012] Optionally, the fluorescence detection module also includes a shell, and the light source assembly, the first dichroic mirror, the second dichroic mirror, the filter assembly and the photoelectric detection assembly are accommodated in the shell, the second direction is parallel to the height axis of the shell, and the first direction and the third direction are perpendicular to the height axis of the shell.

[0013] Optionally, the first dichroic mirror and the second dichroic mirror are arranged on a height axis of the housing, and the light source assembly, the filter assembly and the photoelectric detection assembly are arranged on a plane perpendicular to the height axis.

[0014] Optionally, the fluorescence detection module also includes a first collimating lens and a second collimating lens, the first collimating lens is arranged between the light source assembly and the first dichroic mirror, and is used to collimate the light generated by the light source assembly; the second collimating lens is arranged between the first dichroic mirror and the sample placement side, and is used to collimate the light generated by the light source assembly reflected by the first dichroic mirror and to collimate the fluorescence generated by the sample to be tested.

[0015] Optionally, the fluorescence detection module also includes a first baffle and a second baffle, the first baffle being arranged between the light source assembly and the first dichroic mirror for adjusting the spot of light generated from the light source assembly, and the second baffle being arranged on the height axis of the shell for adjusting the spot of fluorescence generated by the sample to be tested.

[0016] Optionally, the fluorescence detection module further includes a first circuit board and a second circuit board, the light source assembly is arranged on the first circuit board, the photoelectric detection assembly is arranged on the second circuit board, and the first circuit board and the second circuit board are detachably arranged on the housing.

[0017] Optionally, the housing includes a first housing and a second housing, and the first housing and the second housing are detachably connected;

[0018] One of the first circuit board and the second circuit board is arranged at the junction of the first shell and the second shell, and the other of the first circuit board and the second circuit board is arranged on the side surface of the first shell or the second shell.

[0019] The present application also provides a fluorescence detection device, comprising a detection component and the fluorescence detection module as described above, wherein the detection component is used to receive an electrical signal generated after the photoelectric detection component performs photoelectric conversion processing on the filtered fluorescence.

[0020] This application optimizes the optical path in the fluorescence detection module, thereby reducing the size of the fluorescence detection module in the direction from the sample to be tested to the photoelectric detection component. Therefore, the volume of the instrument loaded with the fluorescence detection module can be reduced and the application range of the fluorescence detection module can be expanded. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 Schematic diagram of the three-dimensional structure of the fluorescence detection module provided in one embodiment of the present application Figure 1 ;

[0023] Figure 2 Schematic diagram of the three-dimensional structure of the fluorescence detection module provided in one embodiment of the present application Figure 2 ;

[0024] Figure 3 A schematic diagram of the three-dimensional structure of a housing provided in one embodiment of the present application;

[0025] Figure 4 Schematic diagram of the three-dimensional structure of the fluorescence detection module provided in one embodiment of the present application Figure 3 ;

[0026] Figure 5 for Figure 3 Schematic diagram of the explosion structure;

[0027] Figure 6 for Figure 3 Schematic diagram of the cross-section structure. DETAILED DESCRIPTION

[0028] Specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the described embodiments are merely some, and not all, of the embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the description of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0029] In the description of the present invention, unless otherwise specified or limited, the terms "disposed," "installed," and "connected" should be understood broadly. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; and direct or indirect connections through an intermediary. Those skilled in the art will understand the specific meanings of these terms based on the specific circumstances.

[0030] The directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "front", "back", "top", "bottom", "inside" and "outside" are based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the inventive product is usually placed when in use. They are only for the convenience and simplification of description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operate in a specific direction. Therefore, they should not be understood as limiting the present invention.

[0031] Furthermore, the terms "first," "second," "third," etc., are merely used to distinguish between elements of similar nature, and do not indicate or imply relative importance or a particular order.

[0032] In addition, the terms "comprises," "comprising," or any other variations thereof, are intended to cover a non-exclusive inclusion that may include elements other than the listed elements and may also include additional elements not specifically listed.

[0033] like Figure 1 As shown, the fluorescence detection module includes: a light source assembly 1, a first dichroic mirror 2, a second dichroic mirror 3, a filter assembly 4 and a photoelectric detection assembly 5. The light source assembly 1 is an excitation light source, such as an LED lamp, which is not limited here.

[0034] As one embodiment, the first dichroic mirror 2 is arranged on the output light path of the light source component 1, and is used to reflect the excitation light generated by the light source component 1 in the first direction to the second direction to reach the sample to be tested 99. After being excited by the excitation light, the sample to be tested 99 will emit fluorescence in a specific band and emit it to the second dichroic mirror 3 from the opposite direction of the second direction.

[0035] The second dichroic mirror 3 is used to reflect the fluorescence generated by the sample 99 in a third direction to reach the filter assembly 4. The filter assembly 4 is used to filter background light in the non-fluorescent wavelength range to reduce background light interference. The first and third directions are respectively located in planes perpendicular to the second direction. The photodetection assembly 5 is used to perform photoelectric conversion processing on the fluorescence filtered by the filter assembly 4, thereby converting the filtered fluorescence into an electrical signal.

[0036] With this structure, first dichroic mirror 2 redirects the excitation light generated by light source assembly 1, directing it toward the sample under test along a second direction. Furthermore, fluorescence light travels in a direction opposite to the second direction and reaches second dichroic mirror 3, which redirects the fluorescence generated by the sample under test so that it passes through filter assembly 4 and reaches photodetector assembly 5. This reduces the size of the fluorescence detection module from the sample under test to the photodetector assembly, thereby reducing the size of the instrument housing the fluorescence detection module and expanding its application range.

[0037] As one embodiment, the photoelectric detection component 5 includes a fluorescence collecting lens and a photoelectric detector. The fluorescence collecting lens is used to converge the fluorescence passing through the light filtering component 4 so that the converged fluorescence reaches the photoelectric detector.

[0038] In one embodiment, the first dichroic mirror 2 and the second dichroic mirror 3 are disposed in the optical path of the fluorescence emitted by the sample 99 to be tested. Specifically, the first dichroic mirror 2 is closer to the sample placement side than the second dichroic mirror 3, that is, the first dichroic mirror 2 is located between the sample 99 to be tested and the second dichroic mirror 3. The first dichroic mirror 2 is transmissive to the fluorescence generated by the sample 99 to be tested. Thus, the fluorescence generated by the sample 99 to be tested can pass through the first dichroic mirror 2 and reach the second dichroic mirror 3.

[0039] As one embodiment, the angle between the first direction and the third direction is preferably 90°, but is not limited thereto. The angle between the first direction and the third direction is in the range of 0° to 180°, for example, 0°, 15°, 26°, 35°, 45°, 59°, 60°, 76°, 81°, 92°, 104°, 113°, 120°, 137°, 148°, 154°, 161°, 175°, 180°, etc. By adjusting the angle between the first direction and the third direction, the lateral size of the photoelectric detection module can be adjusted, so that different appearances can be designed as needed to adapt to the installation requirements of different instruments.

[0040] like Figure 2 As shown in one embodiment, Figure 2 The angle between the first and third directions is shown as 0°. In this case, the first direction is positioned lower than the third direction above the sample. Correspondingly, the photodetection assembly 5 is positioned higher than the light source assembly 1 above the sample. It will be appreciated that the first direction can also be positioned higher than the third direction above the sample. In this case, the second dichroic mirror 3 transmits the excitation light.

[0041] like Figure 3As shown in one embodiment, the fluorescence detection module further includes a housing 8, in which the light source assembly 1, the first dichroic mirror 2, the second dichroic mirror 3, the filter assembly 4 and the photoelectric detection assembly 5 are housed. Figure 3 The direction Z shown in FIG. 8 , the second direction is parallel to the height axis of the housing 8 , and the first direction and the third direction are perpendicular to the height axis of the housing 8 . Figure 3 Direction X and direction Y shown in correspond to the first direction and the third direction, respectively.

[0042] exist Figure 3 In the structure shown, the shell 8 is in the shape of a cube. In other embodiments, the shell 8 can also be in other shapes, such as a cylinder, a polyhedron, a sphere, a triangular prism, etc., and can be adjusted according to the angle between the first direction and the third direction, but is not limited to this.

[0043] like Figure 4 As shown, the fluorescence detection module further includes a first collimating lens 6 and a second collimating lens 7. The first collimating lens 6 is disposed between the light source assembly 1 and the first dichroic mirror 2 and is configured to collimate the light generated by the light source assembly 1. It is understood that the first collimating lens 6 is an objective lens and is disposed along a first direction between the light source assembly 1 and the first dichroic mirror 2 to collimate the light generated by the light source assembly 1, thereby causing the first dichroic mirror 2 to reflect the collimated light toward the sample 99 to be measured.

[0044] Please combine Figure 3 、 Figure 5 and Figure 6 As one embodiment, the first dichroic mirror 2 and the second dichroic mirror 3 are arranged on the height axis of the housing 8, and the first dichroic mirror 2 is closer to the sample placement side than the second dichroic mirror 3, that is, the first dichroic mirror 2 is located between the sample 99 to be tested and the second dichroic mirror 3. The light source assembly 1, the filter assembly 4 and the photoelectric detection assembly 5 are arranged on a plane perpendicular to the height axis. It can be understood that the light source assembly 1 and the first dichroic mirror 2 are located on a first plane perpendicular to the height axis, and the second dichroic mirror 3, the filter assembly 4 and the photoelectric detection assembly 5 are located on a second plane perpendicular to the height axis. The first plane is higher than the second plane in the direction of the height axis.

[0045] In one embodiment, a second collimating lens 7 is disposed between the first dichroic mirror 2 and the sample placement side, and is used to collimate the light generated by the light source assembly 1 and reflected by the first dichroic mirror 2, as well as to collimate the fluorescence generated by the sample 99 to be tested. It is understood that the second collimating lens 7 is equivalent to the objective lens described in the background art. The second collimating lens 7 is disposed along the second direction between the first dichroic mirror 2 and the sample placement side, and is used to collimate the light generated by the light source assembly 1 and reflected by the first dichroic mirror 2, as well as to collimate the fluorescence generated by the sample 99 to be tested.

[0046] As one embodiment, the second collimating lens 7 is further used to perform secondary collimation on the light generated by the light source assembly 1 and reflected by the first dichroic mirror 2 and passing through the first collimating lens 6 .

[0047] The fluorescence detection module further includes a first baffle 15 and a second baffle 16. The first baffle 15 is disposed between the light source assembly 1 and the first dichroic mirror 2 along the first direction and is used to adjust the spot of light generated by the light source assembly 1. Furthermore, when a first collimating lens 6 is also disposed in the first direction, the first baffle 15 is disposed between the first collimating lens 6 and the first dichroic mirror 2.

[0048] As one embodiment, a second baffle 16 is provided on the height axis of the housing 8 to adjust the fluorescent spot generated by the sample 99. Furthermore, when a second collimating lens 7 is also provided on the height axis, the second baffle 16 is provided between the second collimating lens 7 and the sample 99.

[0049] As one embodiment, a through hole is provided in the middle of each of the first baffle 15 and the second baffle 16 , and the size of the spot of the excitation light can be adaptively adjusted by changing the inner aperture size of the through hole.

[0050] As one embodiment, the fluorescence detection module further includes a first circuit board 17 and a second circuit board 18. The light source assembly 1 is disposed on the first circuit board 17, and the photoelectric detection assembly 5 is disposed on the second circuit board 18. The first circuit board 17 and the second circuit board 18 are detachably disposed on the housing 8. In this way, when the light source assembly 1 or the photoelectric detection assembly 5 fails, they can be repaired or replaced quickly and easily.

[0051] In one embodiment, the housing 8 includes a first housing 81 and a second housing 82, and the first housing 81 and the second housing 82 are detachably connected. In this way, if any optical component contained in the housing 8, including the light source assembly 1, the first dichroic mirror 2, the second dichroic mirror 3, the filter assembly 4, and the photodetection assembly 5, fails, the first housing 81 and the second housing 82 can be disassembled for targeted repair or replacement.

[0052] In one embodiment, one of the first circuit board 17 and the second circuit board 18 is disposed at the junction of the first housing 81 and the second housing 82, and the other of the first circuit board 17 and the second circuit board 18 is disposed on a side surface of the first housing 81 or the second housing 82. In this manner, the first circuit board 17 and the second circuit board 18 are located on two different side surfaces of the housing 8, respectively, so that the light source assembly 1 and the first dichroic mirror 2 disposed in the first direction do not form structural interference with the second dichroic mirror 3, the filter assembly 4, and the photodetection assembly 5 disposed in the third direction, thereby improving space utilization within the housing 8.

[0053] like Figure 5 or Figure 6 As shown, as one of the embodiments, a filter component fixing structure 83 is also provided in the shell 8. The filter component fixing structure 83 is arranged in a third direction and is used to fix the photoelectric detection component 5 in the shell 8 when the second circuit board 18 is connected to the shell 8.

[0054] The present application also provides a fluorescence detection device, including a detection component and a fluorescence detection module as described above, wherein the detection component is used to receive the electrical signal generated after the photoelectric detection component 5 performs photoelectric conversion processing on the filtered fluorescence. The detection component can be, for example, a photomultiplier tube or a photodiode. It is understood that after the fluorescence reaches the photoelectric detection component 5, the photoelectric detection component 5 is used to perform photoelectric conversion processing on the fluorescence, and then output a corresponding electrical signal, and send the electrical signal to the detection component. The detection component is used to process and analyze the electrical signal, thereby achieving qualitative or quantitative detection of the sample 99 to be tested.

[0055] As one implementation manner, the detection component may be disposed inside the housing 8 or may be disposed outside the housing 8 independently of other optical components, which is not limited herein.

[0056] like Figure 2 and Figure 5 As shown in one embodiment, the fluorescence detection module includes a light source assembly 1, a first dichroic mirror 2, a second dichroic mirror 3, a filter assembly 4, a photodetection assembly 5, a first collimating lens 6, a second collimating lens 7, a first baffle 15, and a second baffle 16, which are housed in a housing 8. The second collimating lens 7, the first dichroic mirror 2, and the second dichroic mirror 3 are arranged on the height axis of the housing 8, the light source assembly 1, the first collimating lens 6, the first baffle 15, and the first dichroic mirror 2 are arranged in a first direction perpendicular to the height axis of the housing 8, and the second dichroic mirror 3, the filter assembly 4, and the photodetection assembly 5 are arranged in a second direction perpendicular to the height axis of the housing 8.

[0057] Furthermore, the plane in which the first direction is located is higher than the plane in which the second direction is located. In addition, the fluorescence detection module further includes a first circuit board 17 and a second circuit board 18 provided on the housing 8 , the light source assembly 1 is provided on the first circuit board 17 , and the photoelectric detection assembly 5 is provided on the second circuit board 18 .

[0058] In actual implementation, firstly, the light source assembly 1 emits excitation light of a specific wavelength band and then collimates the excitation light in a first direction through the first collimating lens 6. The first baffle 15 is used to adjust the spot of the collimated excitation light.

[0059] Secondly, the first dichroic mirror 2 is used to reflect the excitation light, which has passed through the first collimating lens 6 and the first baffle 15, to the second collimating lens 7 for secondary collimation. The secondary collimated excitation light is then directed toward the second baffle 16, which is used to secondary adjust the excitation light spot. The sample 99, excited by the excitation light passing through the second collimating lens 7 and the second baffle 16, produces fluorescence in a specific wavelength band.

[0060] Third, the fluorescence generated by the sample 99 is converged by the second collimating lens 7 onto the second dichroic mirror 3, which then reflects the fluorescence to the filter assembly 4. The filter assembly 4 is used to filter non-fluorescent background light from the fluorescence to reduce background light interference. The photodetector assembly 5 receives the filtered fluorescence and performs photoelectric conversion processing on it to generate an electrical signal.

[0061] Fourthly, the second circuit board 18 amplifies the electrical signal and sends the amplified electrical signal to the detection component, which processes and analyzes the electrical signal to achieve qualitative or quantitative detection of the sample 99 to be tested.

[0062] This application optimizes the optical path in the fluorescence detection module, thereby reducing the size of the fluorescence detection module in the direction from the sample to be tested to the photoelectric detection component. Therefore, the volume of the instrument loaded with the fluorescence detection module can be reduced and the application range of the fluorescence detection module can be expanded.

[0063] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the appended claims.

Claims

1. A fluorescence detection module, characterized in that: include: A light source assembly, a first dichroic mirror, a second dichroic mirror, a filter assembly, and a photoelectric detection assembly; A first dichroic mirror is provided on the outgoing light path of the light source assembly, and is used to reflect the light generated in a first direction by the light source assembly to a second direction so as to reach the sample to be tested; The second dichroic mirror is used to reflect the fluorescence generated by the sample to be tested in a third direction to reach the filter assembly, wherein the first direction and the third direction are respectively located on a plane perpendicular to the second direction; The filter component is used to filter the fluorescence, and the photoelectric detection component is used to perform photoelectric conversion processing on the filtered fluorescence.

2. The fluorescence detection module according to claim 1, characterized in that: The first dichroic mirror and the second dichroic mirror are arranged on the fluorescence emission light path of the sample to be tested. The first dichroic mirror is closer to the sample placement side than the second dichroic mirror. The first dichroic mirror has a transmission effect on the fluorescence generated by the sample to be tested.

3. The fluorescence detection module according to claim 1, characterized in that: The angle between the first direction and the third direction is 90°.

4. The fluorescence detection module according to claim 1, characterized in that: The fluorescence detection module also includes a shell, and the light source assembly, the first dichroic mirror, the second dichroic mirror, the filter assembly and the photoelectric detection assembly are accommodated in the shell, the second direction is parallel to the height axis of the shell, and the first direction and the third direction are perpendicular to the height axis of the shell.

5. The fluorescence detection module according to claim 4, characterized in that: The first dichroic mirror and the second dichroic mirror are arranged on a height axis of the housing, and the light source assembly, the filter assembly and the photoelectric detection assembly are arranged on a plane perpendicular to the height axis.

6. The fluorescence detection module according to claim 5, characterized in that: The fluorescence detection module further includes a first collimating lens and a second collimating lens, wherein the first collimating lens is disposed between the light source assembly and the first dichroic mirror and is used to collimate the light generated by the light source assembly; The second collimating lens is disposed between the first dichroic mirror and the sample placement side, and is used to collimate the light generated by the light source assembly reflected by the first dichroic mirror and to collimate the fluorescence generated by the sample to be measured.

7. The fluorescence detection module according to claim 5, characterized in that: The fluorescence detection module also includes a first baffle and a second baffle. The first baffle is arranged between the light source assembly and the first dichroic mirror, and is used to adjust the spot of light generated by the light source assembly. The second baffle is arranged on the height axis of the shell, and is used to adjust the spot of fluorescence generated by the sample to be tested.

8. The fluorescence detection module according to claim 5, characterized in that: The fluorescence detection module further includes a first circuit board and a second circuit board. The light source assembly is arranged on the first circuit board, and the photoelectric detection assembly is arranged on the second circuit board. The first circuit board and the second circuit board are detachably arranged on the housing.

9. The fluorescence detection module according to claim 8, characterized in that: The housing includes a first housing and a second housing, and the first housing and the second housing are detachably connected; One of the first circuit board and the second circuit board is arranged at the junction of the first shell and the second shell, and the other of the first circuit board and the second circuit board is arranged on the side surface of the first shell or the second shell.

10. A fluorescence detection device, characterized in that: It comprises a detection component and the fluorescence detection module according to any one of claims 1 to 9, wherein the detection component is used to process the electrical signal generated by the photoelectric detection component.

Images