Protein sample purification equipment

Abstract

The utility model discloses protein sample purification equipment which comprises a tank body and a plurality of supporting legs arranged on the side wall of the tank body, and further comprises a purification assembly arranged on the tank body and used for purifying a protein sample, the purification assembly comprises a mounting plate arranged on the inner wall of the tank body, a feeding pipe is arranged on the side, close to the supporting legs, of the mounting plate, and a discharging pipe is arranged on the side, close to the supporting legs, of the mounting plate. The side wall of the feeding pipe is rotationally connected with two connecting pipes which are symmetrically arranged, the ends, away from each other, of the two connecting pipes are fixedly connected with filter cartridges, the side wall of the feeding pipe is provided with a rotating assembly used for rotating the two filter cartridges, and the side, away from the feeding pipe, of the mounting plate is connected with a conveying pipe through a connecting assembly. Through the arrangement of the purification assembly, under the matching action of the rotating assembly and the driving assembly, the protein sample is dynamically filtered, so that the risk that impurities block the filter cartridge is reduced, and the filtering efficiency of the protein sample is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein sample purification, in particular to a protein sample purification device. Background Art

[0002] In the process of using a filter to filter out large particles of impurities in collagen peptides to achieve protein sample separation and purification, the protein solution has a higher viscosity when the concentration is high. In addition, the interaction between protein molecules and the interaction between protein and the filter material will also lead to adhesion, which makes it easy for impurities to adhere to the surface of the filter, causing the filter to be blocked, thereby affecting the quality of protein sample filtration. In addition, protein samples with higher viscosity have a slower flow rate, which increases the resistance to passing through the filter, resulting in a lot of time required for filtration, thereby reducing the efficiency of protein sample purification.

[0003] Therefore, a protein sample purification device is urgently needed to solve the above problems. Utility Model Content

[0004] To achieve the above-mentioned object, the present invention provides the following technical solutions: a protein sample purification device, comprising a tank body and a plurality of support legs arranged on the side wall of the tank body, and further comprising a purification component arranged in the tank body for purifying the protein sample;

[0005] The purification component includes a mounting plate arranged on the inner wall of the tank body, a feed pipe is provided on the side of the mounting plate close to the support leg, the side wall of the feed pipe is rotatably connected to two symmetrically arranged connecting pipes, the ends of the two connecting pipes away from each other are fixedly connected to filter cartridges, the side wall of the feed pipe is provided with a rotating assembly for rotating the two filter cartridges, and the side of the mounting plate away from the feed pipe is connected to a delivery pipe through a connecting assembly.

[0006] The bottom wall of the feed pipe is fixedly connected with a tapered column, and the tapered small end of the tapered column is arranged close to the mounting plate.

[0007] The rotating assembly includes a first bevel gear rotatably connected to the side wall of the feed pipe, and the side walls of the two connecting pipes are fixedly connected to a second bevel gear, the two second bevel gears are meshed with the first bevel gear, and the mounting plate is provided with a driving assembly for driving the first bevel gear.

[0008] The driving assembly includes a driving rod rotatably connected to the mounting plate, the driving rod is fixedly connected to a first sprocket at one end close to the filter cylinder, a mounting ring is provided on the side wall of the feed pipe, one end of the mounting ring is connected to the first bevel gear, the side wall of the mounting ring is fixedly connected to a second sprocket, and the first sprocket and the second sprocket are connected by a chain.

[0009] A motor is provided on one side of the mounting plate away from the filter cartridge, and an output end of the motor is connected to the driving rod.

[0010] The connecting assembly includes a mounting tube arranged on a side of the mounting plate away from the filter cartridge, one end of the mounting tube away from the mounting plate is rotatably connected to a connecting ball, and one end of the feed pipe is connected to the connecting ball.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] The utility model realizes dynamic filtration of protein samples through the arrangement of the purification component, under the cooperation of the rotating component and the driving component, and the impurities originally statically attached to the surface of the filter mesh are subjected to the action of centrifugal force and friction, and their contact with the filter cartridge becomes unstable, which helps to break the adhesion between the impurities and the filter cartridge, thereby reducing the risk of clogging the filter cartridge by impurities. The rotation of the filter cartridge will produce a fluid dynamic effect around it, which is similar to stirring or pumping, and can promote the protein sample to form a stronger flow around the filter cartridge. The enhanced flow helps to overcome the high viscosity of the protein sample, making it easier for it to pass through the pores of the filter cartridge, thereby improving the filtration efficiency of the protein sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the internal structure of the tank body of the present utility model;

[0015] Figure 3 This is a schematic diagram of the purification component structure of the utility model;

[0016] Figure 4 This is a schematic diagram of the tapered column structure of the present utility model.

[0017] In the figure: 101, tank body; 102, support leg; 201, mounting plate; 202, feed pipe; 203, connecting pipe; 204, filter cartridge; 205, conveying pipe; 3, tapered column; 401, first bevel gear; 402, second bevel gear; 501, drive rod; 502, first sprocket; 503, mounting ring; 504, chain; 505, motor; 506, second sprocket; 601, mounting pipe; 602, connecting ball. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] Example 1

[0020] See also Figures 1-4 , a protein sample purification device shown in the figure includes a tank body 101 and a plurality of support legs 102 arranged on the side wall of the tank body 101, and also includes a purification component arranged in the tank body 101 for purifying the protein sample;

[0021] The purification component includes a mounting plate 201 arranged on the inner wall of the tank body 101. A feed pipe 202 is provided on the side of the mounting plate 201 close to the support leg 102. Two symmetrically arranged connecting pipes 203 are rotatably connected to the side wall of the feed pipe 202. The ends of the two connecting pipes 203 away from each other are fixedly connected to filter cartridges 204. A rotating assembly for rotating the two filter cartridges 204 is provided on the side wall of the feed pipe 202. A material delivery pipe 205 is connected to the side of the mounting plate 201 away from the feed pipe 202 via a connecting assembly.

[0022] It should be noted here that: through the setting of the purification component, under the cooperation of the rotating component and the driving component, dynamic filtration of the protein sample is achieved, and the impurities that were originally statically attached to the surface of the filter mesh are no longer in stable contact with the filter cartridge 204 due to the action of centrifugal force and friction, which helps to break the adhesion between the impurities and the filter cartridge 204, thereby reducing the risk of impurities clogging the filter cartridge 204, and the rotation of the filter cartridge 204 will produce a fluid dynamic effect around it. This effect is similar to stirring or pumping, which can promote the protein sample to form a stronger flow around the filter cartridge 204. This enhanced flow helps to overcome the high viscosity of the protein sample, making it easier for it to pass through the pores of the filter cartridge 204, thereby improving the filtration efficiency of the protein sample.

[0023] It is worth noting that the filter cartridge 204 and the connecting pipe 203 are detachably connected.

[0024] See also Figure 4 , the bottom wall of the feed pipe 202 shown in the figure is fixedly connected with a tapered column 3, and the tapered small end of the tapered column 3 is arranged close to the mounting plate 201;

[0025] It should be noted that the conical column 3 is provided to divert the protein sample raw material entering the feed pipe 202 so that it flows into the two filter cartridges 204 in a relatively balanced manner.

[0026] See also Figure 2 and Figure 3 The rotating assembly shown in the figure includes a first bevel gear 401 rotatably connected to the side wall of the feed pipe 202, and the side walls of the two connecting pipes 203 are fixedly connected to the second bevel gears 402. The two second bevel gears 402 are meshed with the first bevel gear 401, and the mounting plate 201 is provided with a driving assembly for driving the first bevel gear 401;

[0027] It should be noted here that the provision of the rotating assembly facilitates the synchronous rotation of the two filter cartridges 204 .

[0028] See also Figure 2 and Figure 3 The driving assembly shown in the figure includes a driving rod 501 rotatably connected to the mounting plate 201, and one end of the driving rod 501 close to the filter cartridge 204 is fixedly connected to the first sprocket 502. A mounting ring 503 is sleeved on the side wall of the feed pipe 202, and one end of the mounting ring 503 is connected to the first bevel gear 401. A second sprocket 506 is fixedly connected to the side wall of the mounting ring 503. The first sprocket 502 and the second sprocket 506 are connected by a chain 504. A motor 505 is provided on the side of the mounting plate 201 away from the filter cartridge 204, and the output end of the motor 505 is connected to the driving rod 501.

[0029] It should be noted here that the arrangement of the driving assembly facilitates the rotation of the first bevel gear 401 .

[0030] Working principle: During the protein sample purification process, the protein sample is first transported to the feed pipe 202 via the feed pipe 205, and then the protein sample flows into the two filter cartridges 204 under the diversion effect of the conical column 3;

[0031] During the process of protein sample raw material flowing into the filter cartridge 204, the motor 505 is started to drive the first sprocket 502 at one end of the driving rod 501 to rotate. During the rotation of the first sprocket 502, the second sprocket 506 is driven to rotate through the chain 504, and then the first bevel gear 401 is driven to rotate. During the rotation of the first bevel gear 401, the two filter cartridges 204 are driven to rotate under the mutual meshing transmission action of the first bevel gear 401 and the two second bevel gears 402. Therefore, during the rotation of the two filter cartridges 204, dynamic filtration of the protein sample is achieved, and the protein sample originally statically attached to the filter cartridge 204 is filtered. The impurities on the surface of the filter mesh are subjected to the effects of centrifugal force and friction, and their contact with the filter cartridge 204 becomes unstable, which helps to break the adhesion between the impurities and the filter cartridge 204, thereby reducing the risk of impurities clogging the filter cartridge 204. In addition, the rotation of the filter cartridge 204 will produce a fluid dynamic effect around it. This effect is similar to stirring or pumping, which can promote the protein sample to form a stronger flow around the filter cartridge 204. This enhanced flow helps to overcome the high viscosity of the protein sample, making it easier for it to pass through the pores of the filter cartridge 204, thereby improving the filtration efficiency of the protein sample.

[0032] Example 2

[0033] See also Figure 4 This embodiment further explains Example 1. The connecting assembly shown in the figure includes a mounting tube 601 disposed on a side of the mounting plate 201 away from the filter cartridge 204. One end of the mounting tube 601 away from the mounting plate 201 is rotatably connected to a connecting ball 602. One end of the feed pipe 205 is connected to the connecting ball 602.

[0034] It should be noted that the connection assembly is provided to enable the delivery tube 205 to be rotatably connected to the mounting plate 201 , thereby facilitating the delivery of protein samples into the feed tube 202 from different directions.

[0035] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A protein sample purification device comprising: A tank body (101) and a plurality of support legs (102) arranged on the side walls of the tank body (101); It is characterized by further comprising: A purification component disposed in the tank (101) for purifying the protein sample; The purification component comprises a mounting plate (201) arranged on the inner wall of the tank body (101); a feed pipe (202) is provided on a side of the mounting plate (201) close to the support leg (102); two connecting pipes (203) symmetrically arranged are rotatably connected to the side wall of the feed pipe (202); one end of the two connecting pipes (203) away from each other is fixedly connected to a filter cartridge (204); a rotating assembly for rotating the two filter cartridges (204) is provided on the side wall of the feed pipe (202); and a side of the mounting plate (201) away from the feed pipe (202) is connected to a delivery pipe (205) via a connecting assembly.

2. A protein sample purification device according to claim 1, characterized in that: A conical column (3) is fixedly connected to the bottom wall of the feed pipe (202), and the conical small end of the conical column (3) is arranged close to the mounting plate (201).

3. A protein sample purification device according to claim 1, characterized in that: The rotating assembly includes a first bevel gear (401) rotatably connected to the side wall of the feed pipe (202), the side walls of the two connecting pipes (203) are fixedly connected with second bevel gears (402), the two second bevel gears (402) are meshed with the first bevel gear (401), and the mounting plate (201) is provided with a driving assembly for driving the first bevel gear (401).

4. A protein sample purification device according to claim 3, characterized in that: The driving assembly includes a driving rod (501) rotatably connected to the mounting plate (201); one end of the driving rod (501) close to the filter cylinder (204) is fixedly connected to a first sprocket (502); a mounting ring (503) is sleeved on the side wall of the feed pipe (202); one end of the mounting ring (503) is connected to the first bevel gear (401); a second sprocket (506) is fixedly connected to the side wall of the mounting ring (503); and the first sprocket (502) and the second sprocket (506) are connected via a chain (504).

5. A protein sample purification device according to claim 4, characterized in that: A motor (505) is provided on a side of the mounting plate (201) away from the filter cartridge (204), and an output end of the motor (505) is connected to the driving rod (501).

6. A protein sample purification device according to claim 1, characterized in that: The connecting assembly comprises a mounting tube (601) arranged on a side of the mounting plate (201) away from the filter cartridge (204); one end of the mounting tube (601) away from the mounting plate (201) is rotatably connected to a connecting ball (602); and one end of the feed pipe (205) is connected to the connecting ball (602).

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