Device for centrifugally culturing cells

Abstract

The utility model relates to a device for centrifugally culturing cells, which comprises a first container, a first centrifugal machine, one or more second containers and one or more second centrifugal machines, the first container and the second containers are mutually independent, the first centrifugal machine is positioned in the first container, and the second centrifugal machines are positioned in the second container. An inlet of the first centrifugal machine is communicated with a cell sap supply unit, an outlet of the first centrifugal machine is communicated with an inlet of the second centrifugal machine, and an outlet of the second centrifugal machine is communicated with an external target cell receiving unit. According to the device for centrifugally culturing the cells provided by the utility model, the first container and the first centrifugal machine can be flexibly selected to carry out centrifugal culture, or the first container, the first centrifugal machine and another one or more second centrifugal machines can be selected to carry out cell culture simultaneously according to actual requirements and the number of required target cells; the number of cells obtained at a time is greatly increased, the cell culture efficiency is improved, meanwhile, flexible combination and matching can be achieved, and the requirement for different cell numbers is met.

Description

Technical Field

[0001] The utility model relates to a device for centrifugally culturing cells. Background Art

[0002] Currently, devices that have both cell centrifugation and culture functions all use a single container. Centrifugation is performed first in the container and then culture is performed directly. Due to factors such as centrifugal stability and temperature control accuracy, the container volume is usually not too large. Only after cells have been cultured in this container can the next batch of centrifugal culture be performed. In this way, the volume of cells cultured in a single centrifugal culture, the number of cells obtained by culture, and the culture efficiency are greatly limited. On the other hand, a single container can only perform centrifugation or culture, and cannot perform both at the same time, which also reduces the culture efficiency. Utility Model Content

[0003] The purpose of the utility model is to provide a device for centrifugally culturing cells.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0005] A device for centrifugal cell culture comprises a first container, a first centrifuge, a second container, and a second centrifuge. The first container and the second container are independent of each other. The first centrifuge is located in the first container, and the second centrifuge is located in the second container. One or more second centrifuges are provided. The inlet of the first centrifuge is used to communicate with a cell fluid supply unit, the outlet of the first centrifuge is communicated with the inlet of the second centrifuge, and the outlet of the second centrifuge is communicated with an external target cell receiving unit.

[0006] According to some implementation aspects of the present invention, a heat dissipation unit is provided in the second container, and the heat dissipation unit includes a shell, a heating rod, a metal sheet, and a fan. The upper and lower sides of the shell are open, the heating rod and the metal sheet are both located in the shell, and the fan is located on the upper side of the shell.

[0007] According to some implementation aspects of the present invention, there are multiple metal sheets, and the multiple metal sheets are arranged in parallel; and\or,

[0008] A convex portion is provided on one side of the outermost metal sheet, and a through hole for accommodating the heating rod is opened on the convex portion.

[0009] According to some implementation aspects of the present invention, a temperature detector is provided in the second container, and the temperature detector is used to detect the temperature in the second container.

[0010] According to some implementation aspects of the present invention, the first container and the second container are located on the same workbench.

[0011] According to some implementation aspects of the present invention, the second container includes a cavity and a cover, the cavity is a cavity with one side open and the other sides closed, the cover is arranged on the open side of the cavity, one end of the cover is rotatably connected to the cavity, and a locking piece is provided outside the cavity, and the locking piece is used to lock the other end of the cover.

[0012] According to some implementation aspects of the present invention, the locking member includes a rotating part and a pressing part, the rotating part is rotatably connected to the cavity, and the pressing part is connected to the upper end of the rotating part; the second container has a locking state, and when in the locking state, the pressing part presses against the other end of the cover body.

[0013] According to some implementation aspects of the present invention, the cover body includes a cover body and an extension part. One end of the cover body is rotatably connected to the workbench, and the extension part is arranged at the other end of the cover body. A downwardly recessed groove is provided on the extension part, and the pressing part of the locking member can press against the bottom wall of the groove.

[0014] According to some implementation aspects of the present invention, a boss is provided on the upper side of the pressing portion.

[0015] According to some implementation aspects of the present invention, the outlet of the first centrifuge is connected to the inlet of the second centrifuge via a connecting pipeline, and a delivery pump is provided on the pipeline.

[0016] Due to the application of the above technical solution, the utility model has the following advantages compared with the prior art:

[0017] The centrifugal cell culture device provided by the present invention can flexibly choose to use only the first container and the first centrifuge for centrifugal culture, or choose to use the first container, the first centrifuge and one or more second centrifuges for cell culture simultaneously, depending on actual needs and the required number of target cells. This greatly increases the number of cells obtained in a single time and improves the efficiency of cell culture. At the same time, it can be flexibly combined to meet different cell quantity requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Attachment Figure 1 This is a cross-sectional view of the device for centrifugal cell culture provided by the present invention.

[0019] Attachment Figure 2 This is a structural diagram from a first perspective of the device for centrifugal cell culture provided by the present invention;

[0020] Attachment Figure 3 This is a front view of the device for centrifugal cell culture provided by the present invention;

[0021] Attachment Figure 4 A top view of the device for centrifugal cell culture provided by the present invention;

[0022] Attachment Figure 5 A structural diagram of the device for centrifugal cell culture provided by the present invention from a second perspective (without the cover);

[0023] Attachment Figure 6 A structural diagram of the centrifugal cell culture device provided by the present invention from a third perspective (without the cover);

[0024] Attachment Figure 7 This is a structural diagram from a first perspective of a heat dissipation unit of the centrifugal cell culture device provided by the present invention;

[0025] Attachment Figure 8 A structural diagram of the heat dissipation unit of the centrifugal cell culture device provided by the present invention from a second perspective;

[0026] Attachment Figure 9 A structural diagram of the heat dissipation unit of the centrifugal cell culture device provided by the present invention from a third perspective;

[0027] Attachment Figure 10 A structural diagram of the heat dissipation unit of the centrifugal cell culture device provided by the present invention from a fourth perspective;

[0028] Attachment Figure 11 A structural diagram of the heat dissipation unit of the centrifugal cell culture device provided by the present invention from a fifth perspective;

[0029] Attachment Figure 12 A structural diagram of a heating rod of the centrifugal cell culture device provided by the present invention;

[0030] Attachment Figure 13 A structural diagram of a locking member of the centrifugal cell culture device provided by the present invention from a first perspective;

[0031] Attachment Figure 14 A structural diagram of a locking member of the centrifugal cell culture device provided by the present invention from a second perspective;

[0032] Attachment Figure 15 A structural diagram of a first-perspective view of a fixing base of the centrifugal cell culture device provided by the present invention;

[0033] Attachment Figure 16 A structural diagram of a second perspective of a fixing base of the centrifugal cell culture device provided by the present invention;

[0034] Attachment Figure 17 This is a structural diagram of the cover of the second container of the centrifugal cell culture device provided by the present invention.

[0035] In the above attached figures:

[0036] 1- first container;

[0037] 2-First centrifuge;

[0038] 3-second container, 31-cavity, 32-cover body, 33-groove;

[0039] 4- Second centrifuge;

[0040] 5-housing; 6-heating rod; 7-metal sheet; 8-convex portion;

[0041] 9-locking member, 91-rotating portion, 92-pressing portion, 93-boss;

[0042] 10-mounting plate, 11-mounting table; 12-temperature protector; 13-workbench; 14-main line; 15-branch line; 16-delivery pump; 17-seat body; 18-mounting part; 19-temperature detector; 20-fan; 21-target cell receiving unit. DETAILED DESCRIPTION

[0043] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0044] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating positions or relationships, are based on the positions or relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0045] See also Figures 1 to 17The device for centrifugal cell culture shown in the figure includes a first container 1, a first centrifuge 2, a second container 3, and a second centrifuge 4. The first container 1 and the second container 3 are independent of each other and are arranged adjacent to each other. The first centrifuge 2 is located in the first container 1, and one or more second centrifuges 4 are provided. The second centrifuge 4 is located in the second container 3. The inlet of the first centrifuge 2 is used to communicate with the cell fluid supply unit, the outlet of the first centrifuge 2 is connected to the inlet of the second centrifuge 4 through a connecting pipe, and the outlet of the second centrifuge 4 is connected to the external target cell receiving unit 21 through a pipe.

[0046] Among them, when the second centrifuge 4 is provided with one, the outlet of the first centrifuge 2 is connected to the inlet of the second centrifuge 4, and the outlet of the second centrifuge 4 is connected to the external target cell receiving unit 21 (cell bag); when the second centrifuge 4 is provided with multiple, multiple second centrifuges 4 are all located in the second container 3, the inlets of the multiple second centrifuges 4 are all connected to the outlet of the first centrifuge 2, and the outlet of the second centrifuge 4 is connected to the external target cell receiving unit 21 (cell bag).

[0047] Inject the sample into the first centrifuge 2 ( Figure 1 After centrifugation, the cells obtained after centrifugation are transferred to the second centrifuge 4 for culture expansion (the culture medium buffer is injected into the second centrifuge 4, Figure 1 Finally, the first centrifuge 2 and the second centrifuge 4 can culture cells at the same time, which increases the culture volume by 2 times compared with the previous one. At the same time, the first centrifuge 2 and the second centrifuge 4 can perform centrifugation and culture respectively, saving the time of centrifugation and culture.

[0048] In a preferred embodiment, two second centrifuges 4 are provided, both of which are located within the second container 3. The inlet of the first second centrifuge 4 is connected to the outlet of the first centrifuge 2, and the outlet of the second centrifuge 4 is connected to the inlet of the adjacent second centrifuge 4. The outlet of the second second centrifuge 4 is connected to the external target cell receiving unit 21. After the sample is injected into the first centrifuge 2, centrifugation is performed, and the cells obtained after centrifugation are transferred to the second centrifuge 4 for culture and expansion. Ultimately, the first centrifuge 2 and the two second centrifuges 4 can simultaneously culture cells, increasing the culture volume by three times compared to the previous method. At the same time, the first centrifuge 2 and the two second centrifuges 4 can perform centrifugation and culture separately, saving centrifugation time.

[0049] In this example, the outlet of the first centrifuge 2 is connected to the inlet of the second centrifuge 4 via a connecting pipeline, which is provided with a delivery pump 16, preferably a peristaltic pump. When multiple second centrifuges 4 are provided, the connecting pipeline includes a main pipeline 14 and multiple branch pipelines 15. One end of the main pipeline 14 is connected to the outlet of the first centrifuge 2, and the other end of the main pipeline 14 is connected to the external target cell receiving unit 21. Each branch pipeline 15 is connected to the main pipeline 14 at one end, and the other end of the branch pipeline 15 is connected to the inlet of the second centrifuge 4.

[0050] In some embodiments, a temperature detector 19 is provided in the second container 3. The temperature detector 19 is used to detect the temperature in the second container 3 to facilitate temperature control in the second container 3. In this example, a fixing base is provided in the second container 3, and the temperature detector 19 is disposed on the fixing base. The fixing base includes a base body 17 and a mounting portion 18 protruding from one side of the base body 17. The mounting portion 18 is provided with a through hole for the temperature detector 19 to pass through.

[0051] In a preferred embodiment, the first container 1 and the second container 3 are located on the same workbench 13, which is convenient for installation and facilitates the communication between the first centrifuge 2 and the second centrifuge 4. The overall structure is compact and occupies a small area.

[0052] In this example, a heat dissipation unit is provided in the second container 3, and the heat dissipation unit includes a shell 5, a heating rod 6, a metal sheet 7, and a fan 20. The shell 5 is connected to the inner wall of the second container 3, and the shell 5 is in the shape of a rectangular parallelepiped. The shell 5 has a accommodating cavity, which is a chamber with both the upper and lower sides open and the other sides closed. The accommodating cavity of the shell 5 is connected to the second container 3, and the heating rod 6 and the metal sheet 7 are both located in the accommodating cavity of the shell 5. The heating rod 6 is powered on to provide heat (there is a resistance wire in the heating rod 6 to generate heat), and the heat is conducted to the metal sheet 7, and the temperature of the metal sheet 7 is increased; the fan 20 is located on the upper side of the shell 5 and above the heating rod 6 and the metal sheet 7. The fan 20 blows air, and blows the heat on the metal sheet 7 into the second container 3 through the air flow, so that the air flow of the second container 3 forms a cycle, and the heat is conducted to the second centrifuge 4.

[0053] In this embodiment, the metal sheet 7 is vertically arranged, and multiple metal sheets 7 are provided. The multiple metal sheets 7 are arranged in parallel, with a gap between adjacent metal sheets 7. In some embodiments, the metal sheets 7 are rectangular; the outermost metal sheet 7 is provided with a protrusion 8 on the side away from the adjacent metal sheet 7. The protrusion 8 is connected to the inner wall of the housing 5 and has a through hole for accommodating the heating rod 6. The protrusion 8 is used to fix the heating rod 6.

[0054] Preferably, a temperature protector 12 is further provided in the housing 5 to prevent the temperature from being too high.

[0055] The device of this example also includes a fan mounting base, which is arranged on the upper side of the housing 5 and can be connected to the housing 5 through fasteners (bolt and nut assemblies). An opening is opened on the fixed mounting base, and the opening is connected to the housing 5. Figure 7-9 The fan mounting base includes a mounting plate 10 and a mounting platform 11. The mounting plate 10 is connected to the upper side of the housing 5. The mounting plate 10 can be in a straight U-shape, which is convenient for fixed connection with the housing 5; the mounting platform 11 is arranged on the side of the mounting plate 10 away from the housing 5. The mounting platform 11 is used to fix the fan 20. Openings are opened on the mounting plate 10 and the mounting platform 11. The openings are connected to the housing 5. Multiple mounting platforms 11 can be provided, and multiple corresponding fans 20 are provided. The fans 20 correspond to the mounting platforms 11 one by one. Providing the mounting platform 11 facilitates the installation of the fan 20.

[0056] The first container 1 of this embodiment is also provided with a temperature detector and a heat dissipation unit. The temperature detector is the same as the temperature detector 19 in the second container 3, and the heat dissipation unit is the same as the heat dissipation unit in the second container 3. The first container 1 includes a cavity and a cover.

[0057] See also Figure 2-6 , 17, the second container 3 includes a cavity 31 and a cover. The cavity 31 is a cavity 31 with one side open and the other sides closed, with the open side facing upward. The cover is arranged on the open side of the cavity 31, and one end of the cover is rotatably connected to the workbench 13. A locking piece 9 is provided on the cavity 31, and the locking piece 9 is used to lock the other end of the cover.

[0058] In some embodiments, the locking member 9 includes a rotating portion 91 and a pressing portion 92. The rotating portion 91 is vertically arranged and rotatably connected to the cavity 31. The pressing portion 92 is horizontally arranged and connected to the upper end of the rotating portion 91 and is vertically arranged. The second container 3 has a locking state and a non-locking state. When in the locking state, the pressing portion 92 presses against the other end of the cover body to lock the cover body; when in the non-locking state, the pressing portion 92 is away from the other end of the cover body, and the other end of the cover body can be opened away from the cavity, so that the cavity is open.

[0059] Preferably, a boss 93 is provided on the upper side of the pressing portion 92, and the boss 93 may be cylindrical. When the cover body is to be opened, the boss 93 may be held to rotate the locking member 9, which can quickly move the locking member 9 away from the cover body. The boss 93 is provided to facilitate the operation of the locking member 9.

[0060] See also Figure 17The cover body includes a cover body 32 and an extension. One end of the cover body 32 is rotatably connected to the cavity. The extension is provided at the other end of the cover body 32. A downwardly recessed groove 33 is formed on the edge of the upper side of the extension. The pressing portion 92 of the locking member 9 can press against the bottom wall of the groove 33. Preferably, two locking members 9 are provided. The front and rear edges of the upper side of the extension are both provided with grooves 33. The two locking members 9 are respectively provided at the front and rear ends of one side of the cover body, and the two locking members 9 respectively cooperate with the two grooves 33.

[0061] In this example, a sealing ring is provided at the contact portion between the cover and the cavity, so that the cover can better seal the cavity. An opening is provided on the sealing ring, and the outlet of the first centrifuge 2 is connected to the inlet of the second centrifuge 4 through a connecting pipe, which can pass through the opening. The pipe connecting the outlet of the second centrifuge 4 and the external target cell receiving unit 21 can pass through the opening.

[0062] The first centrifuge 2 and the second centrifuge 4 in this example are both devices well known to those skilled in the art, and their structures will not be described in detail.

[0063] The specific embodiment of the centrifugal cell culture device of this example is as follows: the first container 1 is in cooling mode, and the internal temperature of the first container 1 is controlled at 4°C ± 0.5°C by a fan and a temperature probe. The blood sample enters the first centrifuge 2 through the lower opening of the first container 1, and the desired cells are obtained by centrifugation to remove the red blood and supernatant. The blood sample is pumped out of the first centrifuge 2 by a pump and injected into two second centrifuges 4 respectively. The culture medium buffer is also injected. The second container 3 is in heating mode, and the internal temperature of the second container 3 is controlled at 37°C ± 0.5°C by a fan 20 and a temperature detector 19. The cells begin to be cultured and expanded in the two second centrifuges 4. At this time, a new sample can be injected into the first centrifuge 2 again, and the desired cells are obtained by centrifugation to remove the red blood and supernatant. The first container 1 is in heating mode, and the internal temperature of the first container 1 is controlled at 37°C ± 0.5°C by a fan and a temperature probe. After the culture medium is injected, culture begins. In this way, the first centrifuge 2 and the two second centrifuges 4 simultaneously carry out cell culture and expansion. Finally, the cells after culture and expansion are pumped into the target cell bag by a pump.

[0064] The centrifugal cell culture device of this example can flexibly choose to use only the first container 1 and the first centrifuge 2 for centrifugal culture, or select the first container 1, the first centrifuge 2 and one or two second centrifuges 4 to carry out cell culture simultaneously, depending on actual needs and the required number of target cells.

[0065] Equipment that requires both centrifugation and cell culture functions usually only has one centrifugal culture container, which limits the number of samples that can be processed and the number of cells that can be cultured and obtained, resulting in low efficiency. The technology of the present invention uses the original container as the main container and adds two additional auxiliary containers to cooperate with the main container for cell culture, greatly increasing the number of cells obtained in a single time and improving the efficiency of cell culture. At the same time, it can be flexibly combined to meet different cell quantity requirements.

[0066] The above embodiments are intended only to illustrate the technical concepts and features of the present invention. Their purpose is to enable those familiar with the art to understand the contents of the present invention and implement them accordingly. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications based on the spirit of the present invention are intended to be included in the scope of protection of the present invention.

Claims

1. A device for centrifugal cell culture, characterized in that: The invention comprises a first container, a first centrifuge, a second container and a second centrifuge. The first container and the second container are independent of each other. The first centrifuge is located in the first container, and the second centrifuge is located in the second container. One or more second centrifuges are provided. The inlet of the first centrifuge is used to communicate with a cell fluid supply unit, the outlet of the first centrifuge is used to communicate with the inlet of the second centrifuge, and the outlet of the second centrifuge is used to communicate with an external target cell receiving unit.

2. The device for centrifugal cell culture according to claim 1, characterized in that A heat dissipation unit is provided in the second container, and the heat dissipation unit includes a shell, a heating rod, a metal sheet, and a fan. The upper and lower sides of the shell are open, and the shell is connected to the second container. The heating rod and the metal sheet are both located in the shell, and the fan is located on the upper side of the shell.

3. The device for centrifugal cell culture according to claim 2, characterized in that: There are multiple metal sheets, and the multiple metal sheets are arranged in parallel, with a gap between two adjacent metal sheets; and\or, A convex portion is provided on one side of the outermost metal sheet, and a through hole for accommodating the heating rod is opened on the convex portion.

4. The device for centrifugal cell culture according to claim 1, characterized in that A temperature detector is provided in the second container, and the temperature detector is used to detect the temperature in the second container.

5. The device for centrifugal cell culture according to claim 1, characterized in that: The first container and the second container are located on the same workbench.

6. The device for centrifugal cell culture according to claim 5, characterized in that: The second container includes a cavity and a cover. The cavity is a cavity with one side open and the other sides closed. The cover is arranged on the open side of the cavity. One end of the cover is rotatably connected to the cavity. A locking piece is arranged outside the cavity, and the locking piece is used to lock the other end of the cover.

7. The device for centrifugal cell culture according to claim 6, characterized in that: The locking member includes a rotating portion and a pressing portion, wherein the rotating portion is rotatably connected to the workbench, and the pressing portion is connected to the upper end of the rotating portion; The second container has a locked state. When in the locked state, the pressing portion presses against the other end of the cover.

8. The device for centrifugal cell culture according to claim 6, characterized in that: The cover body includes a cover body and an extension part. One end of the cover body is rotatably connected to the cavity. The extension part is arranged at the other end of the cover body. A downwardly recessed groove is provided on the extension part. The pressing part of the locking member can press against the bottom wall of the groove.

9. The device for centrifugal cell culture according to claim 7, characterized in that: A boss is provided on the upper side of the pressing portion.

10. The device for centrifugal cell culture according to claim 1, characterized in that: The outlet of the first centrifuge is connected to the inlet of the second centrifuge through a connecting pipeline, and a delivery pump is provided on the connecting pipeline.

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