Ultramicro light splitting device with molecular diagnosis function
Through the integration of spectrometer, molecular diagnostic module and temperature detector, the ultra-micro spectroscopy device solves the problem of single function, realizes the integration of absorbance measurement and molecular diagnosis, improves experimental efficiency and measurement accuracy, and enhances the versatility of the instrument.
Patent Information
- Application Number
- CN202422315444.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-23
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2034-09-23
AI Technical Summary
The existing ultramicro spectrophotometers have a single function and are difficult to meet the needs of multiple applications in the biological and chemical fields, especially in the shortcomings in molecular diagnostics and advanced analytical algorithms.
An ultra-micro spectroscopy device with molecular diagnostic function was designed, integrating a spectrometer, molecular diagnostic module, OD detection module and temperature detector, which realizes the integration of absorbance measurement and molecular diagnostics, and has temperature change path compensation function and dual-channel fluorescence detection capabilities.
It realizes the simultaneous absorption measurement and molecular diagnosis of a single device, improves experimental efficiency, has temperature compensation function to ensure measurement accuracy, and has dual-channel fluorescence detection capabilities, enhancing the practicality and versatility of the instrument.
Smart Images
Figure CN223272416U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of ultra-micro spectroscopic devices, in particular to an ultra-micro spectroscopic device with a molecular diagnosis function. Background Art
[0002] An ultramicro spectrophotometer is an instrument used to measure the spectral characteristics of trace samples and is widely used in biochemistry, molecular biology and other fields. With the continuous development of molecular diagnostic technology, the demand for instruments that can simultaneously perform absorbance measurement and molecular diagnosis is increasing. Therefore, the development of an ultramicro spectrophotometer with molecular diagnostic function is of great practical significance.
[0003] Different application fields may have specific requirements for ultra-micro spectrophotometers. For example, the biological field may need to measure the spectral characteristics of fluorescence or biomolecules, while the chemical field may be more concerned with the absorption spectrum of compounds. Existing ultra-micro spectrophotometers are relatively simple in function. A small number of devices will add OD600 cuvette and fluorometer functions, but do not have other more advanced analysis algorithms. For certain specific application scenarios and multiple application requirements, therefore, an ultra-micro spectrophotometer with molecular diagnostic function is proposed to address the above problems. Utility Model Content
[0004] In order to make up for the deficiencies of the prior art, the utility model proposes an ultra-micro spectroscopic device with a molecular diagnostic function.
[0005] The technical solution adopted by the present invention to solve its technical problems is: an ultra-micro spectrometer with a molecular diagnostic function, comprising an ultra-micro spectrometer, a housing of the ultra-micro spectrometer fixedly connected to a spectrometer, a plug interface of the spectrometer connected to an outgoing light fiber, a sample addition area provided on the upper portion of the outgoing light fiber, an end portion of the outgoing light fiber fixedly connected to an incoming light fiber, an end portion of the incoming light fiber fixedly connected to a transfer fiber, an end portion of the transfer fiber plugged with a controller, a plate of the controller fixedly connected to the bottom of the housing of the ultra-micro spectrometer, a linear motor fixedly connected to the middle portion of the housing of the ultra-micro spectrometer, an output end of the linear motor fixedly connected to a moving arm, a detection platform provided on the upper portion of the moving arm, and the detection platform A lower supporting column is provided on the upper part of the platform, an upper supporting column is fixedly connected to the upper part of the ultra-micro spectrometer housing, an upper detection arm is fixedly connected to the end of the upper supporting column, a baffle is fixedly connected to the side of the movable arm, a position detector is provided on the fixing frame of the movable arm, a temperature detector is provided on the fixing frame of the movable arm, an integrated controller in the middle of the ultra-micro spectrometer is plugged into the opening and closing detector, an OD detection module is plugged into the integrated controller in the middle of the ultra-micro spectrometer, a xenon lamp is provided on the rear side of the OD detection module, a molecular diagnostic module is plugged into the integrated controller in the middle of the ultra-micro spectrometer, a display is fixedly connected to the front side of the ultra-micro spectrometer, and the display is electrically connected to the control circuit of the ultra-micro spectrometer;
[0006] Preferably, the molecular diagnosis module includes: a nucleic acid amplification unit, a molecular diagnosis base, an excitation light unit 1, an optical path convergence filter unit 1, an optical path filter unit 1, an optical path convergence filter unit 2, an optical path acquisition and processing unit 1, an optical path acquisition and processing unit 2, an excitation light unit 2, and an optical path filter unit 2. The nucleic acid amplification unit is provided at the bottom of the molecular diagnosis module, the molecular diagnosis base is provided at the side of the molecular diagnosis module, the inner wall of the molecular diagnosis module is plugged with the optical path filter unit 1, the end of the optical path filter unit 1 is fixedly connected to the excitation light unit 1, the middle of the molecular diagnosis module is fixedly connected to the optical path convergence filter unit 2, the front side of the optical path convergence filter unit 2 is fixedly connected to the optical path acquisition and processing unit 1, the rear side of the molecular diagnosis module is plugged with the optical path convergence filter unit 1, the end of the optical path convergence filter unit 1 is fixedly connected to the optical path acquisition and processing unit 2, the groove on the side of the molecular diagnosis module is plugged with the optical path filter unit 2, the end of the optical path filter unit 2 is fixedly connected to the excitation light unit 2, and the front side of the molecular diagnosis module is plugged with the OD detection module;
[0007] Preferably, the OD detection module includes: an optical excitation unit, an incubation unit, a magnetic stirring unit, an OD base, an optical path convergence unit, a light convergence unit, and an optical filtering unit. The optical path convergence unit is plugged into the front side of the molecular diagnosis module, the end of the optical path convergence unit is plugged into the optical excitation unit, the incubation unit is plugged into the side of the molecular diagnosis module, the magnetic stirring unit is plugged into the socket in the middle of the molecular diagnosis module, the OD base is plugged into the socket in the middle of the molecular diagnosis module, the OD base is plugged into the end of the light convergence unit, and the rear side of the molecular diagnosis module is fixedly connected to the end of the optical filtering unit.
[0008] The utility model is beneficial in that:
[0009] (1) Increased cross-domain instrument functionality: Users do not need to purchase other molecular diagnostic equipment and ultra-micro spectrophotometers to conduct cross-device operations. They can perform absorbance measurement and molecular diagnosis simultaneously, which improves the practicality and versatility of the instrument. One device can directly complete two or more experimental operations, greatly improving experimental efficiency. For example, after the nucleic acid extraction experiment, the nucleic acid concentration can be directly tested with this instrument. After adding the reaction reagent, the experiment can be performed directly on our instrument on the isothermal fluorescence detector (molecular diagnostic function), saving time and equipment.
[0010] (2) Adding an ultra-micro spectrophotometer with a temperature change optical path compensation function: Since the instrument itself is composed of various components, a certain temperature rise will be generated inside the instrument as the use time increases. Since the sample addition area is made of metal material with a thermal expansion coefficient, the optical path will change with the change of temperature. The accuracy of high-concentration measurement is very dependent on the accuracy of the optical path and requires micron-level accuracy. The ultra-micro detection functional area of the present invention has a temperature acquisition sensor, which monitors the temperature of the sample chamber in real time, calculates the optical path change caused by the temperature change based on the temperature signal, and compensates the optical path, thereby achieving the purpose of stabilizing the measurement results.
[0011] (3) Add molecular diagnostic analysis function: The controller controls the molecular diagnostic module to perform molecular diagnosis on the sample, including amplification, fluorescence detection, back-end data processing and analysis, and displays the results on the display screen for easy viewing and operation by users.
[0012] (4) Adding dual-channel fluorescence detection function: When the molecular diagnosis module is added and the temperature control function is not used, this area is a single-hole dual-channel fluorometer.
[0013] (5) Add a molecular diagnostic module and combine it with the OD600 cuvette function module: The optical path signal of the OD600 cuvette module and the optical signal collection and conversion of the molecular diagnostic module are integrated on a circuit for digital-to-analog conversion, and sent to the controller for processing. The OD600 cuvette module has an independent light source, and has a 37°C incubation function and a magnetic stirring function. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0015] Figure 1 This is a schematic diagram of the external structure of the utility model;
[0016] Figure 2 This is a schematic diagram of the internal structure on the right side of the utility model;
[0017] Figure 3 This is a schematic diagram of the left internal structure of the utility model;
[0018] Figure 4 This is a schematic diagram of the internal top view structure of the utility model;
[0019] Figure 5 It is a schematic diagram of the local structure of the utility model.
[0020] In the picture:
[0021] 1. Xenon lamp; 2. Light input fiber; 3. Transfer fiber; 4. Sample loading area; 5. Light output fiber; 6. Spectrometer; 7. Position detector; 8. Linear motor; 9. Opening and closing detector; 10. Molecular diagnostic module; 11. OD600 detection module; 12. Controller; 13. Display; 14. Temperature detector; 15. Baffle; 16. Moving arm; 17. Upper detection arm; 18. Detection platform; 19. Upper support column; 20. Lower support column; 101. Nucleic acid amplification unit; 102. Molecular diagnostic base; 103. Excitation light unit 1; 104. Optical path filtering unit 1; 105. Optical path convergence filtering unit 2; 106. Optical path acquisition and processing unit 1; 107. Optical path acquisition and processing unit 2; 108. Excitation light unit 2; 109. Optical path filtering unit 2; 1010. Optical path convergence filtering unit 1; 111. Optical excitation unit; 112. Incubation unit; 113. Magnetic stirring unit; 114. OD600 base; 115. Optical path convergence unit; 116. Light convergence unit; 117. Optical filtering unit. DETAILED DESCRIPTION
[0022] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0023] The following is combined with Figure 1-5 To further explain this application,
[0024] The embodiment of the present application discloses an ultra-micro spectrometer with a molecular diagnostic function, including an ultra-micro spectrometer, wherein the housing of the ultra-micro spectrometer is fixedly connected to a spectrometer 6, the plug interface of the spectrometer 6 is connected to a light-emitting optical fiber 5, the upper portion of the light-emitting optical fiber 5 is provided with a sample addition area 4, the end of the light-emitting optical fiber 5 is fixedly connected to an input optical fiber 2, the end of the input optical fiber 2 is fixedly connected to a transfer optical fiber 3, the end of the transfer optical fiber 3 is plugged with a controller 12, the plate of the controller 12 is fixedly connected to the bottom of the housing of the ultra-micro spectrometer, the middle portion of the housing of the ultra-micro spectrometer is fixedly connected to a linear motor 8, the output end of the linear motor 8 is fixedly connected to a moving arm 16, the upper portion of the moving arm 16 is provided with a detection platform 18, and the upper portion of the detection platform 18 is provided with a lower support column 20. , an upper support column 19 is fixedly connected to the upper part of the ultra-micro spectrometer housing, an upper detection arm 17 is fixedly connected to the end of the upper support column 19, a baffle 15 is fixedly connected to the side of the movable arm 16, a position detector 7 is provided on the fixed frame of the movable arm 16, a temperature detector 14 is provided on the fixed frame of the movable arm 16, an integrated controller in the middle of the ultra-micro spectrometer is plugged into the opening and closing detector 9, an OD600 detection module 11 is plugged into the integrated controller in the middle of the ultra-micro spectrometer, a xenon lamp 1 is provided on the rear side of the OD600 detection module 11, a molecular diagnostic module 10 is plugged into the integrated controller in the middle of the ultra-micro spectrometer, a display 13 is fixedly connected to the front side of the ultra-micro spectrometer, and the display 13 is electrically connected to the control circuit of the ultra-micro spectrometer;
[0025] The molecular diagnosis module 10 includes: a nucleic acid amplification unit 101, a molecular diagnosis base 102, an excitation light unit 103, a light path convergence filter unit 1010, a light path filter unit 104, a light path convergence filter unit 2 105, a light path acquisition and processing unit 106, a light path acquisition and processing unit 2 107, an excitation light unit 2 108, and a light path filter unit 2 109. The nucleic acid amplification unit 101 is provided at the bottom of the molecular diagnosis module 10, the molecular diagnosis base 102 is provided at the side of the molecular diagnosis module 10, the light path filter unit 104 is plugged into the inner wall of the molecular diagnosis module 10, and the end of the light path filter unit 104 is fixed. The molecular diagnosis module 10 is connected to an excitation light unit 103, the middle of the molecular diagnosis module 10 is fixedly connected to an optical path convergence filter unit 2 105, the front side of the optical path convergence filter unit 2 105 is fixedly connected to an optical path acquisition and processing unit 106, the rear side of the molecular diagnosis module 10 is plugged into an optical path convergence filter unit 1010, the end of the optical path convergence filter unit 1010 is fixedly connected to an optical path acquisition and processing unit 2 107, the groove on the side of the molecular diagnosis module 10 is plugged into an optical path filter unit 2 109, the end of the optical path filter unit 2 109 is fixedly connected to an excitation light unit 2 108, and the front side of the molecular diagnosis module 10 is plugged into an OD600 detection module 11;
[0026] The OD600 detection module 11 includes: an optical excitation unit 111, an incubation unit 112, a magnetic stirring unit 113, an OD600 base 114, a light path convergence unit 115, a light convergence unit 116, and an optical filtering unit 117. The light path convergence unit 115 is plugged into the front side of the molecular diagnosis module 10, and the end of the light path convergence unit 115 is plugged into the optical excitation unit 111. The incubation unit 112 is plugged into the side of the molecular diagnosis module 10, and the magnetic stirring unit 113 is plugged into the socket in the middle of the molecular diagnosis module 10. The OD600 base 114 is plugged into the socket in the middle of the molecular diagnosis module 10, and the OD600 base 114 is plugged into the end of the light convergence unit 116. The rear side of the molecular diagnosis module 10 is fixedly connected to the end of the optical filtering unit 117.
[0027] Working principle: In the specific implementation process, first, distilled water needs to be added to the sample adding area 4, and the blank detection is clicked on the display 13. After the controller 12 controls the linear motor 8 to drive the corresponding baffle 15, the moving arm 16, and the light-emitting optical fiber 5 to reach each predetermined optical path, the controller 12 controls the flashing xenon lamp 1 to turn on the light and pass through the light-input optical fiber 3, the sample adding area 4, and the light-emitting optical fiber 5 in turn, and then uploads the data to the controller 12 for analysis. The data of the five optical paths must be stored in the controller 12 to complete the first step of the detection. The second step is to use dust-free paper to remove the distilled water just now, add the reagent sample and drop it in the sample adding area 4, and click on the sample detection operation on the display 13 (wherein the controller 12 will first collect the temperature of the temperature detector 14 and compare it with the base temperature, and calculate the optical path offset of the sample adding area 4 caused by the temperature change according to the temperature parameters. The step of calculating the optical path offset includes establishing a mathematical model of the temperature and optical path change, and fitting and verifying it through experimental data to automatically compensate for the optical path offset). The controller 12 controls the linear motor 8 to drive the corresponding baffle 15, the moving arm 16, and The output optical fiber 5 first reaches the middle optical path and determines whether the motor moves upward or downward based on the absorbance threshold. The absorbance detected at the appropriate optical path reaches an effective value, and the concentration of the test agent is calculated using the following formula: the flash xenon lamp 1 is used to emit light of all wavelengths; the input optical fiber 2 is used to guide light from the flash xenon lamp into the transfer optical fiber; the transfer optical fiber 3 is used to guide light from the input optical fiber to the sample loading area; the sample loading area 4 is used to carry the test agent; the output optical fiber 5 is used to guide light passing through the sample loading area into the spectrometer; the spectrometer 6 is used to detect the light introduced by the output optical fiber; the position detector 7 is used to detect the position of the movable arm 16 driven by the linear motor, the output optical fiber 5, and the baffle 15; the linear motor 8 is used to move the position of the output optical fiber; the opening and closing detector 9 is used to detect the opening and closing state of the upper detection arm 17 where the transfer optical fiber 3 is located to determine whether detection can be performed; the molecular diagnostic module 10 is used to perform molecular diagnosis on the sample, including nucleic acid amplification and detection; and the OD600 detection module 11 is a detection mechanism for light absorption at a specific wavelength.
[0028] The absorbance of the sample is calculated according to the formula A=lg(1 / T)=Kbc, where A is the absorbance, T is the transmittance, which is the ratio of the outgoing light intensity to the incident light intensity, K is the molar absorptivity, which is related to the sample properties and the wavelength of the incident light, c is the sample concentration in mol / L, and b is the thickness of the absorption layer, i.e., the optical path length.
[0029] In the specific implementation process of the molecular diagnosis function, the test tube of the test reagent needs to be placed in the nucleic acid amplification unit 101, and the display 13 is set to set the operating instructions to heat the surrounding of the adapter to the set temperature. A separate temperature sensor is provided at the bottom of the adapter to collect the temperature in real time. When the preset temperature is reached, the excitation unit 103 emits a specific light, which is injected into the test tube of the test reagent through the optical filter unit 104, and then excites another wavelength of light in a 90-degree optical path direction, which is further injected into the optical path convergence filter unit 2 105 and then collected by the optical path collection and processing unit 106. After processing by the optical acquisition processing unit 2 107, the data is uploaded to the controller 12 for processing and finally uploaded to the display 13 for display to the user. After N cycles, the reagent is continuously amplified, and the fluorescence intensity is continuously increased. An amplification curve relationship is formed with the number of cycles and the relative fluorescence intensity, thereby determining whether it is negative or positive, thereby achieving a diagnostic purpose; when dual-channel detection is required, just open another 90-degree optical path channel to excite the light unit 2 108, which will emit a specific light, pass through the optical filter unit 2 109, and hit the test reagent tube, and then turn the optical path direction at 90 degrees to excite another wavelength of light and further shoot it into the optical path confluence The collection of the optical path is completed by the optical path collection processing unit 2 107 and then uploaded to the controller 12 for processing and finally uploaded to the display 13 for display to the user. The controller 12 is used to control each enabling mechanism, and the display 13 is used to input operation instructions for each function and display data detection results; the temperature detector 14 is used to collect the temperature of the functional area of the spectrophotometer in real time; the baffle 15 is used to sense the position detector 7 to obtain the spacing and optical path between the transfer optical fiber 3 of the sample addition area 4 and the light-emitting optical fiber 5; the moving arm 16 is used to fix the light-emitting optical fiber 5 and the baffle 15 and is driven by the linear motor 8 Drive; the upper detection arm 17 is used to fix the two ends of the transfer optical fiber 3 and is equipped with magnets that can sense the opening and closing detector 9, as well as to fix the upper support column 19; the detection platform 18 is used to fix the moving mechanism associated with the entire light-emitting optical fiber 5, the lower support column 20 and the opening and closing detector 9; the upper support column 19 is installed on the upper detection arm 17 and together with the lower support column 20 completes the control of the relative distance between the upper detection arm 17 and the detection platform 18; the lower support column 20 is installed on the detection platform 18 and together with the upper support column 19 completes the control of the relative distance between the upper detection arm 17 and the detection platform 18.
[0030] During the specific implementation of the OD600 detection module 11 function, first put the empty 10mm standard cuvette into the OD600 base 114, click the display 13 to do a blank control, and then take out the empty 10mm standard cuvette. Further, it is necessary to add the test agent into the 10mm standard cuvette, and then put the cuvette into the OD600 base 114. If incubation and magnetic stirring operations are required, first set the operation instructions on the setting display 13, and incubate at 37 degrees Celsius and the speed and stirring time of magnetic stirring. Further, the controller 12 will control the incubation unit 112 to feedback the real-time temperature through the separate temperature sensor 3. If the preset temperature is not reached, the OD600 base will be set. The heating films on both sides of the seat 114 work until the temperature is reached; and the controller 12 will further control the magnetic stirring unit 113 to rotate the stirring bar at a preset speed, thereby achieving the effect of stirring the reagent; after the current two operations are completed or directly proceed to the next operation, then click on the display 13 to click on the sample test, the controller 12 will control the light excitation unit 111 to emit light of a specific wavelength, pass through the light path convergence unit 115 and the aperture, and then pass through the standard cuvette filled with the reagent, and further pass through the light convergence unit 116 and the light filtering unit 117, and finally be detected by the light path acquisition and processing unit 107 and processed, and sent to the controller 12 for processing and finally uploaded to the display 13 for display to the user.
[0031] The ultra-micro spectrophotometer of the present invention has a molecular diagnostic function and can perform absorbance measurement and molecular diagnosis simultaneously, thereby improving the practicality and versatility of the instrument. The molecular diagnostic module adopts an integrated design of nucleic acid amplification and detection, which is simple and fast to operate and improves detection efficiency. The controller processes and analyzes the detection data and displays the results on the display screen, making it convenient for users to view and operate.
[0032] The ultra-trace detection functional area of the present invention is provided with a temperature acquisition sensor, which monitors the temperature of the sample chamber in real time, calculates the optical path change in the optical path change area caused by the temperature change based on the temperature signal (the step of calculating the optical path change includes establishing a mathematical model of temperature and optical path change, and fitting and verifying it through experimental data), and automatically compensates the optical path, thereby achieving the purpose of stabilizing and accurately measuring results.
[0033] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and improvements are intended to fall within the scope of the present invention.
Claims
1. An ultra-micro spectrometer with molecular diagnostic capabilities, comprising an ultra-micro spectrometer, characterized in that: The housing of the ultra-micro spectrometer is fixedly connected to a spectrometer (6), the plug interface of the spectrometer (6) is connected to an output optical fiber (5), the upper part of the output optical fiber (5) is provided with a sample adding area (4), the end of the output optical fiber (5) is fixedly connected to an input optical fiber (2), the end of the input optical fiber (2) is fixedly connected to a transfer optical fiber (3), the end of the transfer optical fiber (3) is plugged with a controller (12), the plate of the controller (12) is fixedly connected to the bottom of the housing of the ultra-micro spectrometer, the middle part of the housing of the ultra-micro spectrometer is fixedly connected to a linear motor (8), the output end of the linear motor (8) is fixedly connected to a moving arm (16), the upper part of the moving arm (16) is provided with a detection platform (18), the upper part of the detection platform (18) is provided with a lower support column (20), the upper part of the housing of the ultra-micro spectrometer is fixedly connected to the output end of the linear motor ( ... The ultra-micro spectrometer is fixedly connected to an upper support column (19), the end of the upper support column (19) is fixedly connected to an upper detection arm (17), the side of the movable arm (16) is fixedly connected to a baffle (15), the fixed frame of the movable arm (16) is provided with a position detector (7), the fixed frame of the movable arm (16) is provided with a temperature detector (14), the integrated controller in the middle of the ultra-micro spectrometer is plugged into the opening and closing detector (9), the integrated controller in the middle of the ultra-micro spectrometer is plugged into the OD600 detection module (11), the rear side of the OD600 detection module (11) is provided with a flash xenon lamp (1), the integrated controller in the middle of the ultra-micro spectrometer is plugged into the molecular diagnosis module (10), the front side of the ultra-micro spectrometer is fixedly connected to a display (13), and the display (13) is electrically connected to the control circuit of the ultra-micro spectrometer.
2. The ultra-micro spectroscopic device with molecular diagnostic function according to claim 1, characterized in that: The molecular diagnosis module (10) comprises: a nucleic acid amplification unit (101), a molecular diagnosis base (102), an excitation light unit (103), a light path convergence filter unit (1010), a light path filter unit (104), a light path convergence filter unit (105), a light path acquisition and processing unit (106), a light path acquisition and processing unit (107), an excitation light unit (108), and a light path filter unit (109). The lower part of the molecular diagnosis module (10) is provided with the nucleic acid amplification unit (101), the side part of the molecular diagnosis module (10) is provided with the molecular diagnosis base (102), the inner wall of the molecular diagnosis module (10) is plugged with the light path filter unit (104), and the end of the light path filter unit (104) is provided with a plurality of optical paths. The molecular diagnosis module (10) is fixedly connected to an excitation light unit 1 (103); the middle of the molecular diagnosis module (10) is fixedly connected to an optical path convergence filter unit 2 (105); the front side of the optical path convergence filter unit 2 (105) is fixedly connected to an optical path acquisition and processing unit 1 (106); the rear side of the molecular diagnosis module (10) is plugged into an optical path convergence filter unit 1 (1010); the end of the optical path convergence filter unit 1 (1010) is fixedly connected to an optical path acquisition and processing unit 2 (107); the groove on the side of the molecular diagnosis module (10) is plugged into an optical path filter unit 2 (109); the end of the optical path filter unit 2 (109) is fixedly connected to an excitation light unit 2 (108); and the front side of the molecular diagnosis module (10) is plugged into an OD600 detection module (11).
3. The ultra-micro spectroscopic device with molecular diagnostic function according to claim 2, characterized in that: The OD600 detection module (11) comprises: a light excitation unit (111), an incubation unit (112), a magnetic stirring unit (113), an OD600 base (114), a light path convergence unit (115), a light convergence unit (116), and a light filtering unit (117). The light path convergence unit (115) is plugged into the front side of the molecular diagnosis module (10), and the end of the light path convergence unit (115) is plugged into the light excitation unit (111). The incubation unit (112) is plugged into the side of the molecular diagnosis module (10), the magnetic stirring unit (113) is plugged into the socket in the middle of the molecular diagnosis module (10), the OD600 base (114) is plugged into the socket in the middle of the molecular diagnosis module (10), the OD600 base (114) is plugged into the end of the light convergence unit (116), and the rear side of the molecular diagnosis module (10) is fixedly connected to the end of the light filtering unit (117).