Novel nucleic acid extractor

Abstract

The utility model discloses a novel nucleic acid extractor which comprises a bottom plate, a shell, a supporting framework, a sample placing platform and a sample box, the bottom plate is connected with the shell, the supporting framework is connected with the bottom plate, the novel nucleic acid extractor is characterized by further comprising a working mechanism, the working mechanism is connected with the supporting framework through a movement mechanism, the working mechanism comprises a magnetic rod assembly and a magnetic rod sleeve assembly, the magnetic bar sleeve assembly is arranged below the magnetic bar assembly, and the movement mechanism comprises a first lifting assembly used for controlling the magnetic bar assembly to ascend and descend and a second lifting assembly used for controlling the magnetic bar sleeve assembly to ascend and descend. The sample placing platform is connected with the bottom plate through a supporting part, and a heating mechanism for providing heat for the sample box and a positioning mechanism for limiting the movement of the sample box are connected above the sample placing platform. The movement of the magnetic bar component and the magnetic bar sleeve component is controlled through the movement mechanism, and the heating mechanism and the positioning mechanism are arranged above the sample placing platform, so that the sample placing device is simple in structure, low in production cost and convenient to maintain.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid extraction, and in particular relates to a novel nucleic acid extraction instrument. Background Art

[0002] Nucleic acid extractors, which use matching nucleic acid extraction reagents to automatically extract nucleic acid from samples, are widely used in a variety of fields, including disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry, and molecular biology research. The nucleic acid extraction process is complex, and currently, nucleic acid extraction is performed primarily through manual and machine extraction. Manual extraction consumes significant human resources, while machine extraction, while available on the market, is complex, with inefficient coordination and difficulty improving efficiency.

[0003] For example, application number CN202322732324.1 discloses a nucleic acid extraction instrument, in which the first drive component drives the pipetting component to approach or move away from the reaction component, and the second drive component drives the reaction component or the first drive component to move in the first direction, so that the pipetting component moves relative to the reaction component in the first direction, so that the pipetting component can puncture, pipette and mix the reagent tank, nuclear extraction reaction tube and methylation reaction tube, the nuclear extraction temperature control component is used to adjust the temperature of the nuclear extraction reaction tube, and the magnetic adsorption component is used to adsorb magnetic beads in the nuclear extraction reaction tube during the nucleic acid extraction process. The extractor realizes automatic extraction of nucleic acid, but the extractor has a complex structure and high production and maintenance costs. Therefore, it is necessary to develop a nucleic acid extractor with simple structure and easy maintenance. Utility Model Content

[0004] In order to solve the above technical problems, the utility model provides a novel nucleic acid extractor, which optimizes the structure of the novel nucleic acid extractor, reduces production costs, and makes maintenance more convenient.

[0005] Specifically, the utility model discloses a novel nucleic acid extraction instrument, comprising: a bottom plate, a shell, a support frame, a sample placement platform and a sample box, wherein the bottom plate is connected to the shell, and the support frame is connected to the bottom plate, and is characterized in that it also includes:

[0006] A working mechanism, wherein the working mechanism is connected to the supporting frame via a motion mechanism, the working mechanism comprises a magnetic rod assembly and a magnetic rod sleeve assembly, the magnetic rod sleeve assembly is arranged below the magnetic rod assembly, and the motion mechanism comprises a first lifting assembly for controlling the lifting of the magnetic rod assembly and a second lifting assembly for controlling the lifting of the magnetic rod sleeve assembly;

[0007] The sample setting platform is connected to the bottom plate via a supporting portion, and a heating mechanism for providing heat to the sample box and a positioning mechanism for limiting movement of the sample box are connected above the sample setting platform.

[0008] Furthermore, the magnetic bar assembly includes a magnetic bar frame and magnetic bars connected to the magnetic bar frame, and the magnetic bars are detachably arranged below the magnetic bar frame.

[0009] Furthermore, the magnetic rod sleeve assembly includes a magnetic rod sleeve plate frame and a magnetic rod sleeve connected to the magnetic rod sleeve plate frame, the magnetic rod sleeve is connected to the magnetic rod sleeve plate frame through a plug-in portion, and the magnetic rod sleeve plate frame has a slot for matching with the plug-in portion.

[0010] Furthermore, the first lifting assembly includes a first motor, a first screw rod, a screw rod nut and a first slider. The first motor is connected to the support frame, the first screw rod is connected to the first motor, the screw rod nut is threadedly connected to the first screw rod, the first slider is slidably connected to the longitudinal slide rail on one side of the support frame, the screw rod nut is fixedly connected to the first slider through a first connecting plate, and the magnetic rod plate is mounted below the first slider.

[0011] Furthermore, the second lifting assembly includes a second motor, a synchronous pulley, a synchronous belt and a second slider. The second motor is connected to the support frame. There are two synchronous pulleys, one is connected to the second motor, and the other is rotatably connected to the support frame. The synchronous belt is connected to the two synchronous pulleys. The second slider is slidably connected to the longitudinal slide rail, and the second slider is fixedly connected to the synchronous belt through a second connecting plate. The magnetic rod sleeve is mounted below the second slider.

[0012] Furthermore, the motion mechanism also includes a translation assembly, which includes a transverse slide rail, a third motor, and a second screw rod. The third motor and transverse slide rail are arranged above the base plate and fixedly connected to the base plate. The second screw rod is connected to the third motor and is threadedly connected to the support frame. The support frame is slidably connected to the transverse slide rail.

[0013] Furthermore, the support frame is also connected to a column plate, and the column plate has a first limiting groove for limiting the first connecting plate and a second limiting groove for limiting the second connecting plate.

[0014] Furthermore, the heating mechanism includes a heat well, an upper heat insulation plate above the heat well, and a lower heat insulation plate below the heat well, wherein the lower heat insulation plate is arranged above the support portion; wherein,

[0015] The top of the hot well has a groove that matches the bottom of the sample box.

[0016] Furthermore, the upper surface of the upper heat insulation board is also provided with a platform decorative board.

[0017] Furthermore, the positioning mechanism is arranged above the heating mechanism, and includes side positioning plates, a central positioning plate and a plunger ball. The side positioning plates are arranged on both sides of the heating mechanism and placed above the platform decorative plate. The central positioning plate is arranged between the two side positioning plates. The plunger ball is arranged on the inner side of the side positioning plates and on both sides of the central positioning plate.

[0018] The beneficial effects of the present invention are:

[0019] The movement of the magnetic rod assembly and the magnetic rod sleeve assembly is controlled by a motion mechanism, and a heating mechanism and a positioning mechanism are arranged above the layout platform. The structure is simple, the production cost is low, and the maintenance is convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art.

[0021] Figure 1 This is a schematic diagram of a new nucleic acid extraction instrument;

[0022] Figure 2 This is a schematic diagram of the interior of a new nucleic acid extraction instrument;

[0023] Figure 3 This is a schematic diagram of another perspective inside the new nucleic acid extraction instrument;

[0024] Figure 4 This is a schematic diagram of the motion mechanism of the new nucleic acid extraction instrument;

[0025] Figure 5 This is a schematic diagram of the working mechanism of the new nucleic acid extraction instrument;

[0026] Figure 6 This is a schematic diagram of the sample stage;

[0027] Figure 7 This is a schematic diagram of the sample stage with the platform decorative panel hidden.

[0028] The reference numerals in the accompanying drawings are as follows: bottom plate 1, shell 2, support frame 3, longitudinal slide rail 31, column plate 32, first limiting groove 321, second limiting groove 322, sample setting platform 4, support portion 41, sample box 5, working mechanism 6, magnetic rod assembly 61, magnetic rod plate frame 611, magnetic rod 612, magnetic rod sleeve assembly 62, magnetic rod sleeve plate frame 621, magnetic rod sleeve 622, plug-in portion 623, slot 624, motion mechanism 7, first lifting assembly 71, first motor 711, first screw rod 712, First slider 713, first connecting plate 714, screw nut 715, second lifting assembly 72, second motor 721, synchronous pulley 722, synchronous belt 723, second slider 724, second connecting plate 725, translation assembly 73, transverse slide rail 731, third motor 732, second screw 733, heating mechanism 8, heat well 81, groove 811, upper heat insulation plate 82, lower heat insulation plate 83, platform decorative plate 84, positioning mechanism 9, side positioning plate 91, central positioning plate 92, plunger ball 93. DETAILED DESCRIPTION

[0029] The present invention will be further described in detail below with reference to the accompanying drawings.

[0030] like Figure 1-7 As shown, the utility model discloses a novel nucleic acid extraction instrument, comprising a base plate 1, a shell 2, a support frame 3, a sample placement platform 4 and a sample box 5, wherein the base plate 1 is connected to the shell 2, and the support frame 3 is connected to the base plate 1, and is characterized in that it also includes:

[0031] The working mechanism 6 is connected to the supporting frame 3 through the motion mechanism 7. The working mechanism 6 includes a magnetic rod assembly 61 and a magnetic rod sleeve assembly 62. The magnetic rod sleeve assembly 62 is arranged below the magnetic rod assembly 61. The motion mechanism 7 includes a first lifting assembly 71 for controlling the lifting of the magnetic rod assembly 61 and a second lifting assembly 72 for controlling the lifting of the magnetic rod sleeve assembly 62.

[0032] The sample placement platform 4 is connected to the bottom plate 1 via a support portion 41 , and a heating mechanism 8 for providing heat to the sample box 5 and a positioning mechanism 9 for limiting the movement of the sample box 5 are connected above the sample placement platform 4 .

[0033] The utility model controls the lifting and lowering of the working mechanism 6 through the motion mechanism 7 to automatically extract the magnetic beads in the sample box 5. In addition, a heating mechanism 8 is provided at the bottom of the sample platform 4 to heat the sample box 5. A positioning mechanism 9 is provided above the sample platform 4 to facilitate the positioning of the sample box 5. The utility model has a simple structure, low production cost and is easy to maintain.

[0034] The magnetic bar assembly 61 may adopt the following structure, including a magnetic bar frame 611 and a magnetic bar 612 connected to the magnetic bar frame 611 , wherein the magnetic bar 612 is detachably arranged below the magnetic bar frame 611 .

[0035] The magnetic rod sleeve assembly 62 can adopt the following structure, including a magnetic rod sleeve plate frame 621 and a magnetic rod sleeve 622 connected to the magnetic rod sleeve plate frame 621. The magnetic rod sleeve 622 is connected to the magnetic rod sleeve plate frame 621 through a plug-in portion 623, and the magnetic rod sleeve plate frame 621 has a slot 624 for matching with the plug-in portion 623. The magnetic rod sleeve 622 and the plug-in portion 623 are integrally formed, and the setting of the slot 624 facilitates the replacement of the magnetic rod sleeve 622.

[0036] The first lifting assembly 71 in the motion mechanism 7 includes a first motor 711, a first screw rod 712, a screw nut 715, and a first slider 713. The first motor 711 is connected to the support frame 3, the first screw rod 712 is connected to the first motor 711, and the screw nut 715 is threadedly connected to the first screw rod 712. The first slider 713 is slidably connected to the longitudinal slide rail 31 on one side of the support frame 3. The screw nut 715 is fixedly connected to the first slider 713 via a first connecting plate 714. The magnetic rod plate frame 611 is located below the first slider 713. The first motor 711 drives the first screw rod 712 to rotate, causing the screw nut 715 to move up and down, thereby driving the magnetic rod plate frame 611 to rise and fall.

[0037] The second lifting assembly 72 includes a second motor 721, a synchronous pulley 722, a synchronous belt 723, and a second slider 724. The second motor 721 is connected to the support frame 3. There are two synchronous pulleys 722: one connected to the second motor 721 and the other rotatably connected to the support frame 3. The synchronous belt 723 is connected to the two synchronous pulleys 722. The second slider 724 is slidably connected to the longitudinal slide rail 31 and is fixedly connected to the synchronous belt 723 via a second connecting plate 725. The magnetic rod cover frame 621 is located below the second slider 724. The second motor 721 is connected to one synchronous pulley 722 and controls its rotation. Under the action of the other synchronous pulley 722, the synchronous belt 723 moves between the two synchronous pulleys 722. When the synchronous belt 723 moves, the second connecting plate 725 drives the second slider 724 to move up and down, thereby moving the magnetic rod cover frame 621.

[0038] In addition, the motion mechanism 7 also includes a translation assembly 73, which includes a transverse slide 731, a third motor 732, and a second screw 733. The third motor 732 and transverse slide 731 are arranged above the base plate 1 and are fixedly connected to the base plate 1. The second screw 733 is connected to the third motor 732 and is threadedly connected to the support frame 3. The support frame 3 is slidably connected to the transverse slide 731. The third motor 732 controls the rotation of the second screw 733, causing the support frame 3 to slide along the transverse slide 731, thereby changing the position of the magnetic rod 612 and the magnetic rod sleeve 622, facilitating docking with the sample box 5.

[0039] The motion mechanism 7 realizes the longitudinal and lateral movement of the magnetic rod 612 and the magnetic rod sleeve 622 through the motor, the lead screw and the synchronous belt structure, and has a simple structure and is easy to maintain.

[0040] In order to limit the displacement of the magnetic rod 612 and the magnetic rod sleeve 622, a column plate 32 is set on one side of the support frame 3. The column plate 32 has a first limiting groove 321 for limiting the first connecting plate 714 and a second limiting groove 322 for limiting the second connecting plate 725. The first connecting plate 714 is placed in the first limiting groove 321, and the second connecting plate 725 is placed in the second limiting groove 322, which limits the displacement of the first connecting plate 714 and the second connecting plate 725. The structure is simple and easy to maintain.

[0041] The heating mechanism 8 can adopt the following structure, including a heat well 81, an upper heat insulation plate 82 above the heat well 81 and a lower heat insulation plate 83 below the heat well 81, and the lower heat insulation plate 83 is arranged above the support part 41; wherein, the top of the heat well 81 has a groove 811 that matches the bottom of the sample box 5, thereby improving the heating effect of the sample box 5.

[0042] In order to improve the aesthetics of the interior of the novel nucleic acid extractor, a platform decorative plate 84 is further provided on the upper surface of the upper heat insulation plate 82 , and the decorative platform covers the heating mechanism 8 .

[0043] Positioning mechanism 9 is located above heating mechanism 8 and includes side positioning plates 91, a central positioning plate 92, and a plunger ball 93. Side positioning plates 91 are located on either side of heating mechanism 8 and are positioned above platform decorative plate 84. Central positioning plate 92 is located between the two side positioning plates 91. Plunger balls 93 are located inside side positioning plates 91 and on either side of central positioning plate 92. When sample box 5 is placed between side positioning plates 91 and central positioning plate 92, plunger ball 93 engages sample box 5, preventing it from falling off the work platform.

[0044] For those skilled in the art, several modifications and improvements can be made without departing from the inventive concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A novel nucleic acid extraction instrument, comprising a base plate (1), a housing (2), a support frame (3), a sample placement platform (4) and a sample box (5), wherein the base plate (1) is connected to the housing (2), and the support frame (3) is connected to the base plate (1), characterized in that: Also includes: A working mechanism (6), the working mechanism (6) is connected to the supporting frame (3) via a motion mechanism (7), the working mechanism (6) comprises a magnetic rod assembly (61) and a magnetic rod sleeve assembly (62), the magnetic rod sleeve assembly (62) is arranged below the magnetic rod assembly (61), and the motion mechanism (7) comprises a first lifting assembly (71) for controlling the lifting of the magnetic rod assembly (61) and a second lifting assembly (72) for controlling the lifting of the magnetic rod sleeve assembly (62); The sample placement platform (4) is connected to the base plate (1) via a support portion (41), and a heating mechanism (8) for providing heat to the sample box (5) and a positioning mechanism (9) for limiting the movement of the sample box (5) are connected above the sample placement platform (4).

2. The novel nucleic acid extraction instrument according to claim 1, characterized in that: The magnetic bar assembly (61) comprises a magnetic bar frame (611) and a magnetic bar (612) connected to the magnetic bar frame (611), and the magnetic bar (612) is detachably arranged below the magnetic bar frame (611).

3. The novel nucleic acid extraction instrument according to claim 2, characterized in that: The magnetic rod sleeve assembly (62) comprises a magnetic rod sleeve plate frame (621) and a magnetic rod sleeve (622) connected to the magnetic rod sleeve plate frame (621); the magnetic rod sleeve (622) is connected to the magnetic rod sleeve plate frame (621) via a plug-in portion (623), and the magnetic rod sleeve plate frame (621) has a slot (624) for matching with the plug-in portion (623).

4. The novel nucleic acid extraction instrument according to claim 3, characterized in that: The first lifting assembly (71) comprises a first motor (711), a first screw rod (712), a screw nut (715) and a first slider (713); the first motor (711) is connected to the support frame (3); the first screw rod (712) is connected to the first motor (711); the screw nut (715) is threadedly connected to the first screw rod (712); the first slider (713) is slidably connected to the longitudinal slide rail (31) on one side of the support frame (3); the screw nut (715) is fixedly connected to the first slider (713) via a first connecting plate (714); and the magnetic bar plate frame (611) is arranged below the first slider (713).

5. The novel nucleic acid extraction instrument according to claim 4, characterized in that: The second lifting assembly (72) includes a second motor (721), a synchronous pulley (722), a synchronous belt (723) and a second slider (724). The second motor (721) is connected to the support frame (3). There are two synchronous pulleys (722), one of which is connected to the second motor (721) and the other is rotatably connected to the support frame (3). The synchronous belt (723) is connected to the two synchronous pulleys (722). The second slider (724) is slidably connected to the longitudinal slide rail (31), and the second slider (724) is fixedly connected to the synchronous belt (723) through a second connecting plate (725). The magnetic rod sleeve frame (621) is arranged below the second slider (724).

6. The novel nucleic acid extraction instrument according to claim 5, characterized in that: The motion mechanism (7) further comprises a translation assembly (73), the translation assembly (73) comprising a transverse slide rail (731), a third motor (732), and a second screw rod (733), the third motor (732) and the transverse slide rail (731) being arranged above the base plate (1) and fixedly connected to the base plate (1), the second screw rod (733) being connected to the third motor (732) and being threadedly connected to the support frame (3), and the support frame (3) being slidably connected to the transverse slide rail (731).

7. The novel nucleic acid extraction instrument according to claim 6, characterized in that: The support frame (3) is further connected to a column plate (32), and the column plate (32) has a first limiting groove (321) for limiting the first connecting plate (714) and a second limiting groove (322) for limiting the second connecting plate (725).

8. The novel nucleic acid extraction instrument according to claim 1, characterized in that: The heating mechanism (8) comprises a heat well (81), an upper heat insulation plate (82) above the heat well (81), and a lower heat insulation plate (83) below the heat well (81), wherein the lower heat insulation plate (83) is arranged above the support portion (41); wherein, The top of the hot well (81) has a groove (811) that matches the bottom of the sample box (5).

9. The novel nucleic acid extraction instrument according to claim 8, characterized in that: The upper surface of the upper heat insulation board (82) is further provided with a platform decorative board (84).

10. The novel nucleic acid extraction instrument according to claim 9, characterized in that: The positioning mechanism (9) is arranged above the heating mechanism (8), and comprises side positioning plates (91), a central positioning plate (92) and a plunger ball (93). The side positioning plates (91) are arranged on both sides of the heating mechanism (8) and are placed above the platform decorative plate (84). The central positioning plate (92) is arranged between the two side positioning plates (91). The plunger ball (93) is arranged on the inner side of the side positioning plates (91) and on both sides of the central positioning plate (92).

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