Convenient-to-clean filtering device for cell culture

Abstract

The utility model discloses a convenient-to-clean filtering device for cell culture, which belongs to the technical field of cell culture, and comprises a filtering barrel I, a filtering barrel II, a filtering mechanism, a support frame, a cleaning mechanism and a sealing assembly, the filtering barrel II is arranged right above the filtering barrel I, the filtering mechanism is movably connected between the filtering barrel I and the filtering barrel II, and the supporting frame is arranged on the supporting frame. The first filtering barrel, the second filtering barrel and the filtering mechanism are coaxially arranged, a supporting frame is arranged on the left side of the filtering mechanism, a cleaning mechanism is fixedly connected to the top of the front side of the supporting frame, and sealing assemblies are fixedly connected to the edges of the sides, close to the filtering mechanism, of the first filtering barrel and the second filtering barrel. Through the mode, the filtering device for cell culture can clean the filtering mechanism while performing filtering operation, so that blockage of the filtering mechanism is avoided, the working efficiency of the filtering device for cell culture is improved, manual cleaning is avoided, and the labor intensity of workers is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a filter device for cell culture which is easy to clean. Background Art

[0002] With the rapid development of biological science and technology, cell culture technology has become an indispensable tool in life science research, pharmaceutical R&D, and the biotechnology industry. Cell culture, also known as cell cloning technology, refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH, and nutritional conditions) in vitro to enable cells to survive, grow, reproduce, and maintain their primary structure and function. This technology not only provides a wealth of experimental materials for biological research but also provides strong support for the development of pharmaceutical R&D and the biotechnology industry. In the field of cell culture, filtration devices are an indispensable and important tool in the experimental process.

[0003] For example, Chinese utility model patent CN202322336383.7 discloses a cell culture filtration device, which connects a filter barrel to the filtration device by rotation. When filtering the cell culture fluid, the filter barrel can be rotated by pushing the handle, thereby causing the cell culture fluid in the filter barrel to shake, so that the cell culture fluid can quickly pass through the filter screen, avoiding the formation of a liquid film on the filter screen, thereby improving the filtration effect and improving the filtration efficiency.

[0004] However, as filtration time increases, the filter screen or filter element of such a cell culture filtration device will gradually accumulate a large amount of impurities and microorganisms, resulting in a decrease in filtration efficiency and even affecting the quality of cell culture. Users often need to frequently replace the filter screen or filter element, which not only increases experimental costs but may also lead to cell contamination due to untimely replacement. The filter device is very difficult to clean due to its complex structure, which not only increases the difficulty of use for users but also may affect the filtration effect due to incomplete cleaning.

[0005] Based on this, the utility model designs a cell culture filter device that is easy to clean to solve the above problems. Utility Model Content

[0006] In view of the above-mentioned shortcomings of the prior art, the utility model provides a cell culture filtration device that is easy to clean.

[0007] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A cell culture filtration device that is easy to clean, comprising a first filter barrel, a second filter barrel, a filtering mechanism, a support frame, a cleaning mechanism, and a sealing assembly, wherein the second filter barrel is arranged directly above the first filter barrel, the filtering mechanism is movably connected between the first and second filter barrels, the first filter barrel, the second filter barrel, and the filtering mechanism are coaxially arranged, a support frame is provided on the left side of the filtering mechanism, the top of the front side of the support frame is fixedly connected to the cleaning mechanism, and the edges of the first and second filter barrels near the filtering mechanism are fixedly connected to the sealing assembly;

[0009] The filtering mechanism includes a mounting ring 1, a mounting ring 2, a rotating shaft, a filtering assembly and a through hole. The bottom of the mounting ring 1 is slidingly connected to the mounting ring 2, and the mounting ring 1 and the left side of the mounting ring 2 are rotatably connected with the rotating shaft. The interiors of the mounting ring 1 and the mounting ring 2 are both embedded with filtering assemblies, and the right sides of the mounting ring 1 and the mounting ring 2 are both provided with through holes.

[0010] Furthermore, the top end of the rotating shaft is fixedly connected to the inner wall of the filter barrel 2, the bottom end of the rotating shaft is fixedly connected to the inner wall of the filter barrel 1, and the mounting ring 1 and the mounting ring 2 are rotatably connected between the filter barrel 1 and the filter barrel 2 through the rotating shaft.

[0011] Furthermore, the filter assembly includes a mounting frame, a filter screen and a control shaft. The filter screen is fixedly connected to the inner wall of the mounting frame, and the control shaft is fixedly connected to the outer wall on the right side of the mounting frame. The control shaft is rotatably connected in the through hole.

[0012] Furthermore, the filter assembly further includes a pull rod and a rotating wheel. The pull rod is fixedly connected to the right end of the control shaft, and the rotating wheel is fixedly connected to the outer wall of the pull rod.

[0013] Furthermore, the cleaning mechanism includes a fixed cover, a connecting pipe, a solenoid valve, a connecting block, a water diversion pipe and a nozzle. A connecting pipe is fixedly connected to the center position of the top of the fixed cover, a solenoid valve is connected to the pipe wall of the connecting pipe, the right end of the connecting pipe passes through the fixed cover and is fixedly connected to the connecting block, a number of water diversion pipes are fixedly connected to the outer wall of the connecting block at equal intervals around the circumference, and a number of nozzles are fixedly installed at equal intervals on the bottom of the water diversion pipe.

[0014] Furthermore, a dirt storage bucket is fixedly connected to the bottom of the support frame, and a cavity for accommodating a filtering mechanism is provided between the cleaning mechanism and the dirt storage bucket.

[0015] Furthermore, a receiving cavity is provided inside the waste storage barrel, and a sewage discharge pipe is fixedly connected to the bottom of the left side of the waste storage barrel, and the sewage discharge pipe is communicated with the receiving cavity.

[0016] Furthermore, a slide groove is provided inside the sealing component, a baffle is slidably connected in the slide groove, an adjustment groove is provided on the front side of the sealing component, a shift plate is fixedly connected to the outer wall of the baffle, and the shift plate is slidably connected in the adjustment groove.

[0017] Furthermore, a feed port is fixedly connected to the top of the left side of the filter barrel 2, and the feed port is communicated with the interior of the filter barrel 2. A discharge port is fixedly connected to the bottom of the right side of the filter barrel 1, and the discharge port is communicated with the interior of the filter barrel 1.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The utility model is provided with a cleaning mechanism, and uses a nozzle and a water diversion pipe to spray water to clean the filter mechanism, which can effectively remove impurities and microorganisms on the filter mechanism, ensure the filtering effect and extend the service life of the filter mechanism. In addition, when the filter assembly needs to be turned over for more comprehensive cleaning during the cleaning process, the staff can drive the mounting frame and the filter mesh inside it to turn over in the mounting ring 1 or the mounting ring 2 through the control shaft by rotating the rotating wheel. In this way, each side of the filter assembly can be effectively cleaned, which greatly improves the cleaning efficiency and effect. At the same time, the provision of the sewage storage bucket can conveniently collect sewage and impurities generated during the cleaning process, avoiding environmental pollution and inconvenience in cleaning. The utility model not only improves the filtering efficiency and cleaning efficiency, but also ensures the safety and stability of the filtering process.

[0020] 2. The utility model provides a filtering mechanism and utilizes two sets of filtering components provided in the mounting ring 1 or the mounting ring 2 in the filtering mechanism to efficiently filter the cell culture fluid. At the same time, by rotating the control shaft to drive the pull rod to move, the filtering mechanism rotates in the filter barrel 1 and the filter barrel 2, thereby facilitating the cleaning of the filtering components and greatly improving the cleaning efficiency and convenience of use.

[0021] 3. The utility model provides a filtering mechanism, a sealing assembly, and the cooperation of a slide groove and a baffle to achieve a sealed connection between filter barrel 1 and filter barrel 2, thereby preventing the cell culture fluid from leaking during the filtration process and ensuring the stability and safety of the filtration process. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be derived from these drawings without inventive effort.

[0023] Figure 1 This is a three-dimensional diagram of a cell culture filtration device that is easy to clean according to the present invention;

[0024] Figure 2 This is a top view of a cell culture filtration device that is easy to clean according to the present invention;

[0025] Figure 3 To follow Figure 2 AA direction cross-sectional view;

[0026] Figure 4 To follow Figure 2 BB direction cross-sectional view;

[0027] Figure 5 for Figure 3 Enlarged view of point C in the middle;

[0028] Figure 6 for Figure 4 Enlarged view of point D in the middle;

[0029] Figure 7 This is an exploded structural diagram of a filter mechanism in a cell culture filter device that is easy to clean according to the utility model.

[0030] The numbers in the figure represent:

[0031] 1. Filter barrel one; 2. Filter barrel two; 3. Filter mechanism; 31. Mounting ring one; 32. Mounting ring two; 33. Rotating shaft; 34. Filter assembly; 341. Mounting frame; 342. Filter screen; 343. Control shaft; 344. Pull rod; 345. Rotating wheel; 35. Through hole; 4. Support frame; 5. Cleaning mechanism; 51. Fixed cover; 52. Connecting pipe; 53. Solenoid valve; 54. Connecting block; 55. Water diversion pipe; 56. Nozzle; 6. Sewage storage barrel; 61. Accommodating chamber; 62. Sewage discharge pipe; 7. Sealing assembly; 71. Slide; 72. Baffle; 73. Adjusting groove; 74. Diverter plate; 8. Feed port; 9. Discharge port. DETAILED DESCRIPTION

[0032] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0033] Example 1

[0034] In some embodiments, please refer to the appendix of the specification. Figure 1-7 , a cell culture filtration device that is easy to clean, includes a filter barrel 1, a filter barrel 2, a filter mechanism 3, a support frame 4, a cleaning mechanism 5 and a sealing assembly 7. The filter barrel 2 is arranged directly above the filter barrel 1, and the filter mechanism 3 is movably connected between the filter barrel 1 and the filter barrel 2. The filter barrel 1, the filter barrel 2 and the filter mechanism 3 are coaxially arranged. The support frame 4 is provided on the left side of the filter mechanism 3. The cleaning mechanism 5 is fixedly connected to the top of the front side of the support frame 4. The edges of the filter barrel 1 and the filter barrel 2 near the filter mechanism 3 are fixedly connected with the sealing assembly 7.

[0035] The filtering mechanism 3 includes a mounting ring 1 31, a mounting ring 2 32, a rotating shaft 33, a filtering assembly 34 and a through hole 35. The bottom of the mounting ring 1 31 is slidingly connected to the mounting ring 2 32, and the left sides of the mounting ring 1 31 and the mounting ring 2 32 are rotatably connected to the rotating shaft 33. The filtering assembly 34 is embedded in the interior of the mounting ring 1 31 and the mounting ring 2 32, and a through hole 35 is opened on the right side of the mounting ring 1 31 and the mounting ring 2 32.

[0036] In this embodiment, when the filter assembly 34 in the mounting ring 1 31 needs to be cleaned, the pull rod 344 is used to pull and drive the mounting ring 1 31 to rotate on the rotating shaft 33, rotate to the bottom of the cleaning mechanism 5 and be fixed by the support frame 4, and cleaned by the cleaning mechanism 5. At the same time, the mounting ring 2 32 is rotated to between the filter barrel 1 1 and the filter barrel 2 2 to continue the cell culture filtration operation, and the baffle 72 in the sealing assembly 7 at the bottom of the filter barrel 2 2 falls to the top of the mounting ring 2 32 under the action of gravity to seal the sealing device.

[0037] Furthermore, the top end of the rotating shaft 33 is fixedly connected to the inner wall of the filter barrel 2 2, and the bottom end of the rotating shaft 33 is fixedly connected to the inner wall of the filter barrel 1 1. The mounting ring 1 31 and the mounting ring 2 32 are rotatably connected between the filter barrel 1 1 and the filter barrel 2 2 through the rotating shaft 33.

[0038] Furthermore, the filter assembly 34 includes a mounting frame 341, a filter screen 342 and a control shaft 343. The filter screen 342 is fixedly connected to the inner wall of the mounting frame 341, and the control shaft 343 is fixedly connected to the outer wall on the right side of the mounting frame 341. The control shaft 343 is rotatably connected in the through hole 35. During the cleaning process of the cleaning mechanism 5, the filter assembly 34 is driven to flip in the mounting ring 1 31 and the mounting ring 2 32 by rotating the rotating wheel 345 so that both sides of the filter assembly 34 can be effectively cleaned, thereby improving the efficiency and effect of cleaning.

[0039] Furthermore, the filter assembly 34 also includes a pull rod 344 and a rotating wheel 345. The pull rod 344 is fixedly connected to the right end of the control shaft 343, and the rotating wheel 345 is fixedly connected to the outer wall of the pull rod 344. When the filter assembly 34 needs to be flipped over for more comprehensive cleaning, the staff can easily rotate the rotating wheel 345. This action drives the mounting frame 341 and the filter screen 342 inside it to flip over in the mounting ring 1 31 or the mounting ring 2 32 through the control shaft 343. The inner walls of the mounting ring 1 31 and the mounting ring 2 32 are provided with arc grooves that facilitate the rotation of the filter assembly 34. The arc grooves enable it to rotate unimpeded under the rotation of the rotating wheel 345. In this way, each side of the filter assembly 34 can be effectively cleaned, greatly improving the efficiency and effect of cleaning.

[0040] Furthermore, the cleaning mechanism 5 includes a fixed cover 51, a connecting pipe 52, a solenoid valve 53, a connecting block 54, a water diversion pipe 55 and a nozzle 56. The connecting pipe 52 is fixedly connected to the center position of the top of the fixed cover 51, and the solenoid valve 53 is connected to the pipe wall of the connecting pipe 52. The right end of the connecting pipe 52 passes through the fixed cover 51 and is fixedly connected to the connecting block 54. A number of water diversion pipes 55 are fixedly connected to the outer wall of the connecting block 54 at equal intervals around the circumference, and a number of nozzles 56 are fixedly installed at equal intervals at the bottom of the water diversion pipe 55. The opening and closing of the connecting pipe 52 is controlled by the solenoid valve 53, and the cleaning water is introduced into the connecting block 54 through the connecting pipe 52, and the cleaning water is introduced into the nozzle 56 through the water diversion pipe 55. The filter assembly 34 in the mounting ring 1 31 or the mounting ring 2 32 is flushed and cleaned through the nozzle 56.

[0041] Furthermore, a dirt storage bucket 6 is fixedly connected to the bottom of the support frame 4 , and a cavity for accommodating the filtering mechanism 3 is provided between the cleaning mechanism 5 and the dirt storage bucket 6 .

[0042] Furthermore, a accommodating chamber 61 is opened inside the sewage storage barrel 6, and a sewage discharge pipe 62 is fixedly connected to the bottom of the left side of the sewage storage barrel 6. The sewage discharge pipe 62 is connected to the accommodating chamber 61. The sewage storage barrel 6 is used to collect the cleaned sewage and discharge it through the sewage discharge pipe 62 to keep the entire device clean.

[0043] Furthermore, a slide groove 71 is provided inside the sealing component 7, and a baffle 72 is slidably connected to the slide groove 71. An adjustment groove 73 is provided on the front side of the sealing component 7. A dial plate 74 is fixedly connected to the outer wall of the baffle 72, and the dial plate 74 is slidably connected to the adjustment groove 73. The sealing component 7 is a fixed annular plate fixed to the outer wall of the filter barrel 1 and the filter barrel 2 near one end of the filter mechanism 3. The bottom of the annular plate abuts against the filter mechanism 3. When the filter mechanism 3 rotates for cleaning, the sealing of the interior of the filter device is guaranteed to prevent leakage of culture solution or other liquids, thereby To maintain the purity and stability of the cell culture environment, the baffle 72 is driven to slide up and down in the slide groove 71 by toggling the dial plate 74, thereby controlling the opening and closing of the sealing assembly 7. When the mounting ring 1 31 rotates between the filter barrel 1 1 and the filter barrel 2 2, the baffle 72 on the top of the filter barrel 1 is sealed. When the mounting ring 1 31 rotates to just below the cleaning mechanism 5, the baffle 72 on the top of the filter barrel 1 falls back into the slide groove 71. At the same time, the mounting ring 2 32 is located between the filter barrel 1 1 and the filter barrel 2 2, and the baffle 72 on the bottom of the filter barrel 2 2 is sealed.

[0044] Furthermore, a feed port 8 is fixedly connected to the top of the left side of the filter barrel 2, and the feed port 8 is communicated with the interior of the filter barrel 2. A discharge port 9 is fixedly connected to the bottom of the right side of the filter barrel 1, and the discharge port 9 is communicated with the interior of the filter barrel 1. The cell culture to be filtered is introduced into the filter barrel 2 through the feed port 8, filtered by the filtering mechanism 3 between the filter barrel 2 and the filter barrel 1, and then falls into the filter barrel 1, and is finally discharged through the discharge port 9.

[0045] The working principle of the present invention is as follows: the cell culture to be filtered is introduced into the filter barrel 2 through the feed port 8, filtered through the filter mechanism 3 between the filter barrel 2 and the filter barrel 1, and then falls into the filter barrel 1, and is finally discharged through the discharge port 9. When the filter assembly 34 in the mounting ring 1 needs to be cleaned, the mounting ring 1 31 is pulled and driven on the rotating shaft 33 by the pull rod 344, rotated to the right below the cleaning mechanism 5 and fixed by the support frame 4, the opening and closing of the connecting pipe 52 is controlled by the solenoid valve 53, the cleaning water is introduced into the connecting block 54 through the connecting pipe 52, and the cleaning water is introduced into the nozzle 56 through the water guide pipe 55, and then the cleaning water is discharged. The nozzle 56 flushes and cleans the filter assembly 34 in the mounting ring 1 31. During the cleaning process of the cleaning mechanism 5, the filter assembly 34 is driven to flip in the mounting ring 1 31 and the mounting ring 2 32 by rotating the rotating wheel 345 so that both sides of the filter assembly 34 can be effectively cleaned, thereby improving the efficiency and effect of cleaning. The cleaned cell culture falls into the sewage storage barrel 6, and at the same time, the mounting ring 2 32 rotates to between the filter barrel 1 1 and the filter barrel 2 2 to continue the cell culture filtration operation, and the baffle 72 in the sealing assembly 7 at the bottom of the filter barrel 2 2 falls to the top of the mounting ring 2 32 under the action of gravity to seal the filter device.

[0046] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A cell culture filtration device that is easy to clean, comprising a filter barrel (1), a filter barrel (2), a filter mechanism (3), a support frame (4), a cleaning mechanism (5) and a sealing assembly (7), characterized in that: A filter barrel 2 (2) is provided directly above the filter barrel 1 (1), a filter mechanism (3) is movably connected between the filter barrel 1 (1) and the filter barrel 2 (2), the filter barrel 1 (1), the filter barrel 2 (2) and the filter mechanism (3) are coaxially arranged, a support frame (4) is provided on the left side of the filter mechanism (3), a cleaning mechanism (5) is fixedly connected to the top of the front side of the support frame (4), and a sealing assembly (7) is fixedly connected to the edges of the filter barrel 1 (1) and the filter barrel 2 (2) on the side close to the filter mechanism (3); The filtering mechanism (3) comprises a first mounting ring (31), a second mounting ring (32), a rotating shaft (33), a filtering assembly (34) and a through hole (35); the bottom of the first mounting ring (31) is slidably connected to the second mounting ring (32); the first mounting ring (31) and the left side of the second mounting ring (32) are rotatably connected to the rotating shaft (33); the filtering assembly (34) is embedded in the interior of the first mounting ring (31) and the second mounting ring (32); and the right side of the first mounting ring (31) and the second mounting ring (32) are both provided with a through hole (35).

2. The easy-to-clean cell culture filtration device according to claim 1, characterized in that: The top end of the rotating shaft (33) is fixedly connected to the inner wall of the second filter barrel (2), and the bottom end of the rotating shaft (33) is fixedly connected to the inner wall of the first filter barrel (1). The first mounting ring (31) and the second mounting ring (32) are rotatably connected between the first filter barrel (1) and the second filter barrel (2) via the rotating shaft (33).

3. The easy-to-clean cell culture filtration device according to claim 2, characterized in that: The filter assembly (34) comprises a mounting frame (341), a filter screen (342) and a control shaft (343); the filter screen (342) is fixedly connected to the inner wall of the mounting frame (341); the control shaft (343) is fixedly connected to the outer wall on the right side of the mounting frame (341); and the control shaft (343) is rotatably connected to the through hole (35).

4. The easy-to-clean cell culture filtration device according to claim 3, characterized in that: The filter assembly (34) further comprises a pull rod (344) and a rotating wheel (345), wherein the pull rod (344) is fixedly connected to the right end of the control shaft (343), and the rotating wheel (345) is fixedly connected to the outer wall of the pull rod (344).

5. The easy-to-clean cell culture filtration device according to claim 1, characterized in that: The cleaning mechanism (5) comprises a fixed cover (51), a connecting pipe (52), a solenoid valve (53), a connecting block (54), a water diversion pipe (55) and a nozzle (56); the connecting pipe (52) is fixedly connected at the center position of the top of the fixed cover (51); the solenoid valve (53) is connected to the pipe wall of the connecting pipe (52); the right end of the connecting pipe (52) passes through the fixed cover (51) and is fixedly connected to the connecting block (54); a plurality of water diversion pipes (55) are fixedly connected to the outer wall of the connecting block (54) at equal intervals around the circumference; and a plurality of nozzles (56) are fixedly installed at equal intervals on the bottom of the water diversion pipe (55).

6. The easy-to-clean cell culture filtration device according to claim 1, characterized in that: A dirt storage bucket (6) is fixedly connected to the bottom of the support frame (4), and a cavity for accommodating the filtering mechanism (3) is provided between the cleaning mechanism (5) and the dirt storage bucket (6).

7. The easy-to-clean cell culture filtration device according to claim 6, characterized in that: The interior of the sewage storage barrel (6) is provided with a receiving chamber (61), and the bottom of the left side of the sewage storage barrel (6) is fixedly connected with a sewage discharge pipe (62), and the sewage discharge pipe (62) is communicated with the receiving chamber (61).

8. The easy-to-clean cell culture filtration device according to claim 1, characterized in that: A sliding groove (71) is provided inside the sealing assembly (7), a baffle (72) is slidably connected inside the sliding groove (71), an adjustment groove (73) is provided on the front side of the sealing assembly (7), a shift plate (74) is fixedly connected to the outer wall of the baffle (72), and the shift plate (74) is slidably connected inside the adjustment groove (73).

9. The easy-to-clean cell culture filtration device according to claim 1, characterized in that: The top of the left side of the filter barrel 2 (2) is fixedly connected with a feed port (8), and the feed port (8) is communicated with the interior of the filter barrel 2 (2). The bottom of the right side of the filter barrel 1 (1) is fixedly connected with a discharge port (9), and the discharge port (9) is communicated with the interior of the filter barrel 1 (1).

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