Sulfur dioxide detection teaching and research device based on microfluidic technology

Abstract

The utility model provides a sulfur dioxide detection teaching and research device based on a micro-fluidic technology. The sulfur dioxide detection teaching and research device comprises a micro-fluidic chip, two Y-shaped double-helix liquid mixing channels are arranged in the micro-fluidic chip, one end of one Y-shaped double-helix liquid mixing channel is connected with a first sample storage pool and a second sample storage pool which are arranged at one end of the micro-fluidic chip, and the other end of the Y-shaped double-helix liquid mixing channel is connected with the reaction pool. The device can realize a multi-stage reaction process through a plurality of sample storage pools and reaction channels, and through a sampling support and a mobile phone APP photographing collection method, the photographing height is controlled to unify variables, and a mobile phone can freely move to make sampling recording sites free. In a multi-step mixing reaction, a small step has a problem to cause result failure, but the device can collect sample points in a recording process for analysis, so that the analysis and summarization of the result are facilitated, the problem step with the result failure is found out, the result analysis of the whole experiment is more comprehensive, and the whole experiment is controlled.

Description

Technical Field

[0001] The utility model relates to a sulfur dioxide detection teaching and research device based on microfluidic technology. Background Art

[0002] Monitoring sulfur dioxide is an important task related to public health and environmental protection. Existing sulfur dioxide detection methods mainly include ultraviolet spectroscopy, chromatography, and wet chemical methods. Although these methods can provide accurate detection results, they generally have shortcomings such as complex equipment, high operating technical requirements, and long detection cycles. In terms of rapid detection, although there are some portable devices such as electrochemical sensors and paper-based analytical devices, they usually face problems such as low cost, poor stability, or environmental pollution. Microfluidic technology requires trace amounts of reagents, rapid detection, and accurate reaction results, and has the advantages of being environmentally friendly and accurate. CN217473570U discloses a micro-mixing chip for colorimetric detection. By processing multiple first, second, and third sample reservoirs and multiple Y-shaped double-helix channels, a molybdenum antimony anti-mixing color developer and a phosphate solution are added to the multiple first and second sample reservoirs on the chip, respectively. The mixture is mixed in the Y-shaped double-helix channels, and color development is completed in the multiple third sample reservoirs. After color development, the phosphate content is measured using a smartphone colorimetric analysis method. The combination of the microfluidic chip and smartphone shortens the operation time and reagent consumption of the traditional molybdenum blue colorimetric method, eliminates errors caused by differences in color development time, and enhances the cutting-edge and contemporary nature of chemical experiment teaching content. However, the cover slide needs to be opened during the addition process, which allows the reagent to come into contact with air, affecting the reaction results. Utility Model Content

[0003] In order to solve the above technical problems, the utility model provides a sulfur dioxide detection teaching and research device based on microfluidics technology.

[0004] The utility model is achieved through the following technical solutions.

[0005] The utility model provides a teaching and research device for sulfur dioxide detection based on microfluidic technology, comprising a microfluidic chip; two Y-shaped double-helix mixing liquid channels are provided in the microfluidic chip, one end of one Y-shaped double-helix mixing liquid channel is connected to a first sample storage pool and a second sample storage pool provided at one end of the microfluidic chip, and the other end is connected to a reaction pool; one end of the other Y-shaped double-helix mixing liquid channel is respectively connected to the reaction pool and the third sample storage pool, and the other end is connected to a color development pool provided at the other end of the microfluidic chip; the reaction pool and the third sample storage pool are provided in the middle of the microfluidic chip, the color development pool, the first sample storage pool, the second sample storage pool, and the third sample storage pool are respectively connected to an injection hole, the other ends of all the injection holes extend out of their ends adjacent to the microfluidic chip, and a sampling rack is also provided on the microfluidic chip.

[0006] The microfluidic chip includes a sealing plate and a reaction plate. The injection hole, color development pool, first sample storage pool, second sample storage pool, reaction pool, third sample storage pool, and Y-shaped double-helix mixing channel are all processed on the end face of the reaction plate. The sealing plate covers the reaction plate and the two end faces are tightly attached to form a seal.

[0007] The reaction plate is uniformly processed with a plurality of through holes, and the sealing plate is processed with countersunk holes at positions corresponding to the through holes. Bolts are assembled in the through holes on the reaction plate and the countersunk holes on the sealing plate.

[0008] The sampling rack includes a top plate and a support plate. Two parallel support plates are vertically fixed to the two sides below the top plate. The top plate is processed with a first sampling hole and a second sampling hole. The first sampling hole and the second sampling hole are respectively arranged above two Y-shaped double-helix mixing channels.

[0009] The bottom of the support plate is provided with a plurality of pins, and the plurality of pins are assembled in corresponding sockets processed on the sealing plate.

[0010] The plug pins are square and tightly matched with the sockets.

[0011] The lengths of the connecting channels from the first sample storage pool to the second sample storage pool, the reaction pool, and the third sample storage pool to the Y-shaped double-helix liquid mixing channel are the same.

[0012] The beneficial effects of the present invention are: through multiple sample storage pools and reaction channels, the device can realize a multi-stage reaction process, and through the method of sampling bracket plus mobile phone APP photo collection, the photo height is controlled to make the variables unified, and the mobile phone can be moved freely so that the sampling and recording sites are free. In a multi-step mixing reaction, a problem in a small step in the middle can often lead to failure of the result. The present device can collect sample points in the recording process for analysis, which is convenient for the analysis and summary of the results, and finds out the problem steps of the failure of the results, so that the result analysis of the entire experiment is more comprehensive and the overall situation can be controlled. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural diagram of the utility model;

[0014] Figure 2 This is a schematic diagram of the reaction plate structure of the utility model;

[0015] Figure 3 This is a schematic diagram of the reaction pool structure of the utility model;

[0016] Figure 4 This is a schematic diagram of the sealing plate structure of the present utility model;

[0017] Figure 5 This is a schematic structural diagram of the sampling rack of the present utility model.

[0018] In the figure: 1-sealing plate, 2-reaction plate, 3-through hole, 4-injection hole, 5-ventilation hole, 6-color development pool, 7-first sample storage pool, 8-second sample storage pool, 9-reaction pool, 10-third sample storage pool, 11-Y-type double-helix mixing channel, 12-top plate, 13-first sampling hole, 14-syringe, 15-support plate, 16-second sampling hole, 17-pin, 18-jack. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is further described below, but the scope of protection claimed is not limited to the described solution.

[0020] This device is suitable for food safety, environmental monitoring, public health, and clinical applications, enabling rapid and accurate detection of sulfur dioxide under various conditions. The device combines microfluidic chip technology with the image processing capabilities of a smartphone, achieving efficient, low-cost, and user-friendly operation.

[0021] The sulfur dioxide detection device comprises a sealing plate, a reaction plate, microchannels, multiple sample reservoirs, a double-helix mixing channel, an injector, a sampling rack, a reaction cell, and a color development cell. The sealing plate and reaction plate are hot-pressed at a constant temperature of 95°C for 10 minutes to form a sealed reaction environment. Microchannels connect the sample reservoirs and reaction cells to precisely control the transfer of reagents. The device uses microfluidics to connect the sample reservoirs, reaction cells, and color development cell on the reaction plate, enabling trace addition and mixing of reagents. One end of the injection port connects to the reaction cell, while the other end connects to the color development cell via a double-helix mixing channel, ensuring sufficient reagent reaction and color development. The device also includes a data analysis module connected to a smartphone. Sampling variables are controlled by the sampling rack. The smartphone, placed on the sampling rack, captures color changes in the color development cell using a camera and analyzes the RGB values ​​through an app, enabling rapid data processing and output of results. The smartphone's data processing module uses the formula Y = 0.299R + 0.587G + 0.114BY = 0.299R + 0.587G + 0.114BY = 0.299R + 0.587G + 0.114B to convert the RGB values ​​into grayscale values ​​YY, which are then used to calculate the sulfur dioxide concentration. The sulfur dioxide detection device's microfluidic chip technology optimizes liquid flow and reaction, significantly improving detection efficiency and consistency while reducing reagent consumption and mitigating environmental impact. Its portable design makes it suitable for student education and popular science applications.

[0022] Microfluidics technology significantly reduces the amount of reagents required while maintaining reaction sensitivity and precision. Microfluidic chips can complete complex chemical reactions in a very small volume, making the entire detection process more efficient and environmentally friendly.

[0023] This application uses a lock core hole and a locking method using lock core screws and nuts to ensure that the reaction reagents do not leak out of the reaction channel and mixing channel, thereby ensuring the precision of the instrument. The effect is better than directly hot pressing the reaction plate and the sealing plate, while the cost is still relatively low.

[0024] This device combines a rapid chemical reaction with the instant image processing of a smartphone, enabling rapid detection and providing accurate results. The smartphone camera captures the color change after the reaction, and dedicated software analyzes the resulting RGB values. The formula Y = 0.299R + 0.587G + 0.114BY = 0.299R + 0.587G + 0.114B is then used to convert the RGB values ​​into grayscale values ​​(YY), allowing for rapid calculation of sulfur dioxide concentration.

[0025] The entire device is designed with portability and ease of use in mind, making it easy for non-professionals to operate and particularly suitable for rapid on-site testing. This is particularly important for users who need to perform rapid testing in a variety of environments.

[0026] By eliminating the need for expensive laboratory equipment and reagents, the invention significantly reduces operating costs, making it particularly suitable for teaching and research, enhancing experimental interactivity and data analysis capabilities for students and researchers. Furthermore, the use of common polymethyl methacrylate (PMMA) as a chip material further reduces production costs, making this new detection device highly competitive in the market.

[0027] The use of microfluidic chips significantly reduces the generation of chemical waste, complies with current environmental protection standards, and has little impact on the environment.

[0028] A teaching and research device for sulfur dioxide detection based on microfluidic technology comprises a microfluidic chip; two Y-shaped double-helix mixing channels 11 are provided in the microfluidic chip, one end of one Y-shaped double-helix mixing channel 11 is connected to a first sample reservoir 7 and a second sample reservoir 8 arranged at one end of the microfluidic chip, and the other end is connected to a reaction pool 9; one end of the other Y-shaped double-helix mixing channel 11 is respectively connected to the reaction pool 9 and the third sample reservoir 10, and the other end is connected to a color development pool 6 arranged at the other end of the microfluidic chip; the reaction pool 9 and the third sample reservoir 10 are arranged in the middle of the microfluidic chip, the color development pool 6, the first sample reservoir 7, the second sample reservoir 8, and the third sample reservoir 10 are respectively connected to an injection hole 4, and the other ends of all the injection holes 4 extend out of their ends adjacent to the microfluidic chip, the injection hole (4) is designed to be 0.2 mm wide, connected to the first sample reservoir 7, the second sample reservoir 8, and the third sample reservoir 10, allowing the reagent to move accurately in a controlled environment. The reaction pool 9 and the color development pool 6 are used for reaction and display of the color change of the reaction results respectively. The color development pool is designed to facilitate observation and analysis. The diameter of the air permeable micropore 5 is a microporous channel less than 0.2 mm, which has the function of balancing the air pressure in the plate during sampling.

[0029] Furthermore, a sampling rack is also provided on the microfluidic chip. Using the sampling rack and a mobile phone AP to take photos and collect data, the camera height is controlled to make the variables uniform, and the mobile phone can be freely moved to allow for free sampling and recording locations.

[0030] Furthermore, the microfluidic chip includes a sealing plate 1 and a reaction plate 2. The sample inlet 4, color development cell 6, first sample reservoir 7, second sample reservoir 8, reaction cell 9, third sample reservoir 10, and Y-shaped double-helix mixing channel 11 are all machined onto the end faces of the reaction plate 2. The sealing plate 1 overlies the reaction plate 2, with both end faces tightly attached to form a seal. Integrating the sample reservoir and mixing channel into the reaction plate 2 simplifies the manufacturing process and, combined with the sealing plate, creates a closed reaction environment, ensuring that chemical reactions proceed without external interference.

[0031] Furthermore, the reaction plate 2 is uniformly processed with a plurality of through holes 3 , and the sealing plate 1 is processed with countersunk holes at positions corresponding to the through holes 3 , and bolts are assembled in the through holes 3 on the reaction plate 2 and the countersunk holes on the sealing plate 1 .

[0032] Furthermore, the sampling rack includes a top plate 12 and a support plate 15. Two parallel support plates 15 are vertically fixed to the bottom of the top plate 12. The top plate 12 is machined with a first sampling hole 13 and a second sampling hole 15, which are respectively located above the two Y-shaped double-helix mixing channels 11. When sampling with a mobile phone, the phone is placed on the top plate 12, and the camera is placed above the first and second sample reservoirs 7 and 8. Then, according to the reagent transportation and reaction process, the camera is translated along the first sampling hole 13 to the top of the second sampling hole 15 until the reaction is completed.

[0033] Furthermore, the bottom of the support plate 15 is provided with a plurality of pins 17, which fit into corresponding holes 18 machined into the sealing plate 1. The pins 17 are square and tightly fit into the holes 18. Inserting the pins into the holes secures the sampling rack to the chip, simplifying the chip structure and reducing processing costs.

[0034] Furthermore, in order to ensure that the reagents are fully mixed during the reaction, the lengths of the connecting channels from the first sample reservoir 7 and the second sample reservoir 8 as well as the reaction reservoir 9 and the third sample reservoir 10 to the Y-shaped double-helix mixing channel 11 are the same.

[0035] The operating process of using this device is:

[0036] Step 1: Clean the sealing plate 1 and reaction plate 2 with dust-free lens paper, pass the lock core screw through the lock core hole 3, and lock the microfluidic chip reaction plate with the lock core nut.

[0037] Step 2: Use a 0.3*25mm needle syringe to inject sulfur dioxide extract into the first sample storage tank 7.

[0038] Step 3: In the same way, inject the hydrochloric acid fuchsin color development solution into the second sample storage tank.

[0039] Step 4: The two reagents are mixed in the double-helix mixing channel and then moved to the reaction pool. A mobile phone photo is taken to collect the RGB values.

[0040] Step 5: Inject 0.9 g / L formaldehyde buffer solution and NaOH solution into the sample storage tank (10), mix them and move them to the color development tank for color reaction.

[0041] Step 6: After 5-10 minutes of reaction, use mobile phone software to photograph the color development pool under natural light conditions and analyze the RGB value through the software.

[0042] Step 7: Apply the formula Y=0.299R+0.587G+0.114BY=0.299R+0.587G+0.114B to convert RGB into grayscale value YY to complete the determination of residual sulfur dioxide content.

Claims

1. A sulfur dioxide detection teaching and research device based on microfluidics technology, comprising a microfluidic chip, characterized in that: Two Y-shaped double-helix mixing channels (11) are provided in the microfluidic chip. One end of one Y-shaped double-helix mixing channel (11) is connected to a first sample storage pool (7) and a second sample storage pool (8) provided at one end of the microfluidic chip, and the other end is connected to a reaction pool (9); one end of the other Y-shaped double-helix mixing channel (11) is respectively connected to the reaction pool (9) and the third sample storage pool (10), and the other end is connected to a color development pool (6) provided at the other end of the microfluidic chip; the reaction pool (9) and the third sample storage pool (10) are provided in the middle of the microfluidic chip, the color development pool (6), the first sample storage pool (7), the second sample storage pool (8), and the third sample storage pool (10) are respectively connected to an injection hole (4), and the other ends of all the injection holes (4) extend out of their ends adjacent to the microfluidic chip. A sampling rack is also provided on the microfluidic chip.

2. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 1, characterized in that: The microfluidic chip comprises a sealing plate (1) and a reaction plate (2); the injection hole (4), the color development pool (6), the first sample storage pool (7), the second sample storage pool (8), the reaction pool (9), the third sample storage pool (10), and the Y-shaped double-helix mixing channel (11) are all processed on the end face of the reaction plate (2); the sealing plate (1) covers the reaction plate (2) and the two end faces are tightly attached to form a seal.

3. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 2, characterized in that: The reaction plate (2) is uniformly machined with a plurality of through holes (3), the sealing plate (1) is machined with countersunk holes at positions corresponding to the through holes (3), and bolts are assembled in the through holes (3) on the reaction plate (2) and the countersunk holes on the sealing plate (1).

4. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 1, characterized in that: The sampling rack comprises a top plate (12) and a support plate (15), wherein two parallel support plates (15) are vertically fixed to the lower sides of the top plate (12), and a first sampling hole (13) and a second sampling hole (16) are processed on the top plate (12), and the first sampling hole (13) and the second sampling hole (16) are respectively arranged above two Y-shaped double-helix mixing channels (11).

5. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 4, characterized in that: The bottom of the support plate (15) is provided with a plurality of pins (17), and the plurality of pins (17) are assembled in corresponding sockets (18) processed on the sealing plate (1).

6. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 5, characterized in that: The plug pin (17) is square and tightly fits the socket (18).

7. The microfluidic technology-based sulfur dioxide detection teaching and research device according to claim 1, characterized in that: The lengths of the connecting channels from the first sample storage pool (7), the second sample storage pool (8), the reaction pool (9), and the third sample storage pool (10) to the Y-shaped double-helix liquid mixing channel (11) are the same.

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