Phosphorylated antibody purification device

Abstract

The utility model discloses a phosphorylated antibody purification device, which relates to the technical field of antibody purification and comprises a purification box and a plurality of stirring rods, a conical purification cylinder is arranged in the purification box, a screening plate is fixedly sleeved on the side wall of the bottom of the conical purification cylinder, and each stirring rod is arranged above the screening plate. According to the utility model, through the arrangement of the conical purification cylinder and the plurality of stirring rods, a phosphorylated antibody material is smoothly scattered downwards through the conical purification cylinder, and the conical purification cylinder rotates through the driving mechanism, so that the contact area between the phosphorylated antibody and the plurality of first screening holes can be increased; a transmission mechanism can drive a plurality of stirring rods to stir the phosphorylated antibodies scattered to the top of a screening plate again, so that the phosphorylated antibodies can be subjected to secondary screening purification, the phosphorylated antibodies meeting the requirements are prevented from being scattered to the bottom of a conical purification barrel, and the purification effect and the purification efficiency of the phosphorylated antibodies are improved. And raw materials are wasted.

Description

Technical Field

[0001] The utility model relates to the technical field of antibody purification, in particular to a phosphorylated antibody purification device. Background Art

[0002] Antibodies are a type of immunoglobulin that can specifically bind to antigens. The preparation of antibodies involves the process of antibody purification, which requires the use of antibody purification equipment.

[0003] After searching, an antibody purification device for accelerating antibody purification speed with announcement number CN220047378U was found, which includes a box body, the top of the box body is connected to the box body, the top of the inner cavity of the box body is connected to a stepper motor by bolts, the rotating shaft of the stepper motor is connected to a filter box by bolts, both sides of the top of the inner cavity of the filter box are connected to hydraulic rods by bolts, the bottom of the hydraulic rod is connected to an extrusion plate by bolts, both sides and the rear side of the filter box are fixedly inlaid with a first filter screen, and the inner cavity of the filter box is connected to a second filter screen by bolts.

[0004] However, the existing antibody purification device for accelerating antibody purification has certain disadvantages when used. Although the device can discharge the antibody raw material onto the surface of the first filter and the second filter, the antibody raw material easily accumulates on the surface of the first filter and the second filter, making it difficult to increase the contact area between the antibody raw material and the multiple filter holes, resulting in low purification effect and efficiency. In addition, there is a lack of a stirring mechanism inside the antibody raw material, resulting in some antibody raw materials that meet the requirements still remaining above the first filter and the second filter, which will cause material waste. Utility Model Content

[0005] In view of the above problems existing in the existing antibody purification device for accelerating the purification speed of antibodies, the present utility model is proposed.

[0006] Therefore, the purpose of the present invention is to provide a phosphorylated antibody purification device, which solves the problem that the antibody raw material is easily accumulated on the surface of the first filter and the second filter, making it difficult to increase the contact area between the antibody raw material and the multiple filter holes, resulting in low purification effect and efficiency. In addition, there is a lack of a stirring mechanism inside the antibody raw material, resulting in some antibody raw materials that meet the requirements still remaining above the first filter and the second filter, which will cause waste of raw materials.

[0007] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0008] A phosphorylated antibody purification device comprises a purification box and multiple stirring rods, wherein a conical purification cylinder is provided inside the purification box, a plurality of first sieve holes are opened inside the conical purification cylinder, a sieve plate is fixedly sleeved on the bottom side wall of the conical purification cylinder, a plurality of second sieve holes are opened inside the sieve plate, an annular limiting plate is fixedly provided on the top of the sieve plate, a driving mechanism is provided on the side wall of the purification box, the annular limiting plate matches the driving mechanism, two rotating rods are rotatably provided inside the upper part of the purification box, a plurality of stirring rods are respectively fixedly provided on the rod walls of the corresponding rotating rods, each stirring rod is provided above the sieve plate, the rod walls of the two rotating rods are jointly provided with a transmission mechanism, and the transmission mechanism is connected to the driving mechanism through the driving mechanism.

[0009] Preferably, the driving mechanism includes a motor, a driving rod, a first gear and an outer gear ring. The motor is fixedly arranged on the top of the purification box, and the driving rod is rotatably arranged on the top inner wall of the purification box. One end of the driving rod passes through the purification box and is fixedly connected to the output shaft of the motor. The first gear is fixedly sleeved on the rod wall of the driving rod, and the outer gear ring is fixedly sleeved on the outer wall of the annular limiting plate. The first gear is meshed with the outer gear ring.

[0010] Preferably, the transmission mechanism includes two second gears, two pulleys and a belt, the two second gears are fixedly sleeved on the rod walls of the left rotating rod and the driving rod respectively, the two second gears are meshed and connected, one end of the two rotating rods passes through the purification box and is fixedly sleeved on the inside of the corresponding pulley, and the two pulleys are connected by belt transmission.

[0011] Preferably, a support plate is fixedly provided on the inner wall of the purification box, an annular groove is provided on the side wall of the support plate, the cross-section of the annular groove is T-shaped, and a plurality of T-shaped sliders are fixedly provided on the bottom of the screening plate, and each of the T-shaped sliders is slidably embedded in the interior of the corresponding annular groove.

[0012] Preferably, a feed pipe is fixedly sleeved on the upper interior of the purification box, the belt is movably arranged on the outside of the feed pipe, and a control valve is rotatably arranged on the lower side wall of the feed pipe.

[0013] Preferably, an annular discharge port is provided inside the support plate, and a material receiving box is placed on the bottom inner wall of the purification box, and the material receiving box is arranged directly below the annular discharge port.

[0014] In the above technical solution, the technical effects and advantages provided by the utility model are:

[0015] 1. In the present invention, by providing a conical purification cylinder and multiple stirring rods, the phosphorylated antibody material is smoothly scattered downward through the conical purification cylinder. The conical purification cylinder is rotated by a driving mechanism, thereby increasing the contact area between the phosphorylated antibody and the multiple first sieve holes, thereby improving the purification effect and efficiency of the phosphorylated antibody. The transmission mechanism can drive the multiple stirring rods to stir the phosphorylated antibody scattered to the top of the sieve plate again, thereby performing secondary screening and purification of the phosphorylated antibody, thereby preventing the phosphorylated antibody that meets the requirements from being scattered to the bottom of the conical purification cylinder, causing waste of raw materials.

[0016] 2. The utility model sets up a driving mechanism, which includes a motor, a driving rod, a first gear and an outer gear ring. The starting motor can drive the driving rod to rotate. When the driving rod rotates, it can drive the first gear at the bottom to rotate. When the first gear rotates, it can drive the outer gear ring to rotate, thereby realizing the smooth rotation of the annular limit plate and the conical purification cylinder.

[0017] 3. The utility model sets up a transmission mechanism, which includes two second gears, two pulleys and a belt. When the driving rod rotates, it can drive the corresponding second gear to rotate. When the second gear rotates, it can drive the adjacent second gear to rotate, thereby realizing the smooth rotation of the left rotating rod. Through the transmission of the two pulleys and the belt, the smooth rotation of the two rotating rods and multiple stirring rods is realized. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0019] Figure 1 This is a schematic cross-sectional view of the utility model;

[0020] Figure 2 For the utility model Figure 1 A magnified view of part A;

[0021] Figure 3 For the utility model Figure 1 Schematic diagram of the three-dimensional connection structure of the conical purification cylinder and the screening plate.

[0022] Description of reference numerals:

[0023] 1. Purification box; 2. Stirring rod; 3. Conical purification cylinder; 4. First sieve hole; 5. Sieve plate; 6. Second sieve hole; 7. Annular limit plate; 8. Rotating rod; 9. Motor; 10. Driving rod; 11. First gear; 12. Outer gear ring; 13. Second gear; 14. Pulley; 15. Belt; 16. Support plate; 17. T-shaped slider; 18. Feed pipe; 19. Control valve; 20. Annular discharge port; 21. Material receiving box. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0025] The embodiment of the utility model discloses a phosphorylated antibody purification device.

[0026] Example 1

[0027] The utility model provides Figure 1-3 The phosphorylated antibody purification device shown in the figure includes a purification box 1 and multiple stirring rods 2, characterized in that a conical purification cylinder 3 is provided inside the purification box 1, a plurality of first sieve holes 4 are opened inside the conical purification cylinder 3, a sieve plate 5 is fixedly sleeved on the bottom side wall of the conical purification cylinder 3, a plurality of second sieve holes 6 are opened inside the sieve plate 5, and an annular limiting plate 7 is fixedly provided on the top of the sieve plate 5. A driving mechanism is provided on the side wall of the purification box 1, and the annular limiting plate 7 matches the driving mechanism. Two rotating rods 8 are rotatably provided inside the upper part of the purification box 1, and multiple stirring rods 2 are fixedly provided. On the rod wall corresponding to the rotating rod 8, each stirring rod 2 is arranged above the screening plate 5, and the rod walls of the two rotating rods 8 are jointly provided with a transmission mechanism. The transmission mechanism and the driving mechanism are connected through the driving mechanism. The conical purification cylinder 3 and multiple stirring rods 2 are provided, thereby improving the purification effect and efficiency of the phosphorylated antibody. The transmission mechanism can drive the multiple stirring rods 2 to stir the phosphorylated antibodies scattered to the top of the screening plate again, thereby performing secondary screening and purification of the phosphorylated antibodies, and preventing the phosphorylated antibodies that meet the requirements from being scattered to the bottom of the conical purification cylinder 3, causing waste of raw materials.

[0028] In order to realize the smooth rotation of the annular limiting plate 7 and the conical purification cylinder 3, as shown in FIG. Figure 1-3As shown, the driving mechanism includes a motor 9, a driving rod 10, a first gear 11 and an outer gear ring 12. The motor 9 is fixedly arranged on the top of the purification box 1, and the driving rod 10 is rotatably arranged on the top inner wall of the purification box 1. One end of the driving rod 10 passes through the purification box 1 and is fixedly connected to the output shaft of the motor 9. The first gear 11 is fixedly sleeved on the rod wall of the driving rod 10, and the outer gear ring 12 is fixedly sleeved on the outer wall of the annular limiting plate 7. The first gear 11 is meshed with the outer gear ring 12. The driving mechanism is set to realize the smooth rotation of the annular limiting plate 7 and the conical purification cylinder 3.

[0029] In order to realize the smooth rotation of the two rotating rods 8 and the plurality of stirring rods 2, as shown in FIG. Figure 1-2 As shown, the transmission mechanism includes two second gears 13, two pulleys 14 and a belt 15. The two second gears 13 are fixedly sleeved on the rod wall of the left rotating rod 8 and the driving rod 10 respectively, and the two second gears 13 are meshed and connected. One end of the two rotating rods 8 passes through the purification box 1 and is fixedly sleeved on the inside of the corresponding pulley 14. The two pulleys 14 are connected by the belt 15. The transmission mechanism is set up to achieve smooth rotation of the two rotating rods 8 and multiple stirring rods 2.

[0030] Example 2

[0031] On the basis of the first embodiment, in order to support the conical purification cylinder 3 and the sieve plate 5 and ensure that the conical purification cylinder 3 and the sieve plate 5 can rotate stably, as shown in FIG. Figure 1-3 As shown, a support plate 16 is fixedly provided on the inner wall of the purification box 1, and an annular chute is provided on the side wall of the support plate 16. The cross-section of the annular chute is T-shaped, and a plurality of T-shaped sliders 17 are fixedly provided on the bottom of the screening plate 5. Each T-shaped slider 17 is slidably embedded in the interior of the corresponding annular chute. The T-shaped sliders 17 are provided to support the conical purification cylinder 3 and the screening plate 5, thereby ensuring that the conical purification cylinder 3 and the screening plate 5 can rotate stably.

[0032] Example 3

[0033] On the basis of Example 1, in order to smoothly discharge the phosphorylated antibody raw material to the side wall of the conical purification cylinder 3, as shown in FIG. Figure 1-3 As shown, a feed pipe 18 is fixedly sleeved on the upper interior of the purification box 1, a belt 15 is movably arranged on the outside of the feed pipe 18, and a control valve 19 is rotatably arranged on the lower side wall of the feed pipe 18. By utilizing the set feed pipe 18, the phosphorylated antibody raw material can be smoothly discharged to the side wall of the conical purification cylinder 3.

[0034] Finally, in order to collect the purified phosphorylated antibodies, Figure 1-2As shown, an annular discharge port 20 is provided inside the support plate 16, and a receiving box 21 is placed on the bottom inner wall of the purification box 1. The receiving box 21 is arranged directly below the annular discharge port 20. The purified phosphorylated antibodies can be collected by utilizing the provided annular discharge port 20 and the receiving box 21.

[0035] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A phosphorylated antibody purification device, comprising a purification box (1) and a plurality of stirring rods (2), characterized in that: The purification box (1) is provided with a conical purification cylinder (3) inside, and a plurality of first sieve holes (4) are opened inside the conical purification cylinder (3). A sieve plate (5) is fixedly sleeved on the bottom side wall of the conical purification cylinder (3), and a plurality of second sieve holes (6) are opened inside the sieve plate (5). An annular limiting plate (7) is fixedly provided on the top of the sieve plate (5). The side wall of the purification box (1) is provided with a driving mechanism, and the annular limiting plate (7) matches the driving mechanism. Two rotating rods (8) are rotatably provided inside the upper part of the purification box (1), and a plurality of stirring rods (2) are respectively fixedly provided on the rod wall of the corresponding rotating rod (8). Each stirring rod (2) is provided above the sieve plate (5). The rod walls of the two rotating rods (8) are jointly provided with a transmission mechanism, and the transmission mechanism is connected to the driving mechanism through the driving mechanism.

2. The phosphorylated antibody purification device according to claim 1, characterized in that: The driving mechanism comprises a motor (9), a driving rod (10), a first gear (11) and an outer gear ring (12); the motor (9) is fixedly arranged on the top of the purification box (1); the driving rod (10) is rotatably arranged on the top inner wall of the purification box (1); one end of the driving rod (10) passes through the purification box (1) and is fixedly connected to the output shaft of the motor (9); the first gear (11) is fixedly sleeved on the rod wall of the driving rod (10); the outer gear ring (12) is fixedly sleeved on the outer wall of the annular limiting plate (7); and the first gear (11) is meshedly connected with the outer gear ring (12).

3. The phosphorylated antibody purification device according to claim 1, characterized in that: The transmission mechanism includes two second gears (13), two pulleys (14) and a belt (15). The two second gears (13) are fixedly sleeved on the rod wall of the left rotating rod (8) and the driving rod (10), respectively. The two second gears (13) are meshed and connected. One end of the two rotating rods (8) passes through the purification box (1) and is fixedly sleeved on the inside of the corresponding pulley (14). The two pulleys (14) are connected by the belt (15).

4. The phosphorylated antibody purification device according to claim 3, characterized in that: A support plate (16) is fixedly provided on the inner wall of the purification box (1), and an annular chute is provided on the side wall of the support plate (16). The cross section of the annular chute is T-shaped. A plurality of T-shaped sliders (17) are fixedly provided on the bottom of the screening plate (5), and each of the T-shaped sliders (17) is slidably embedded in the interior of the corresponding annular chute.

5. The phosphorylated antibody purification device according to claim 3, characterized in that: A feed pipe (18) is fixedly sleeved on the upper interior of the purification box (1), the belt (15) is movably arranged on the outside of the feed pipe (18), and a control valve (19) is rotatably arranged on the lower side wall of the feed pipe (18).

6. The phosphorylated antibody purification device according to claim 4, characterized in that: An annular discharge port (20) is provided inside the support plate (16), and a material receiving box (21) is placed on the bottom inner wall of the purification box (1), and the material receiving box (21) is arranged directly below the annular discharge port (20).

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