Culture medium replacement device for immune cell proliferation

Abstract

The utility model discloses a culture medium replacement device for immune cell proliferation, which comprises a water bath box provided with a plurality of grooves, and further comprises a support frame which moves in the grooves and is used for bearing a culture tube, the support frame is divided into an annular cylinder and a vertical cylinder which is annularly arranged on the outer side of the culture tube, and a liquid storage cavity is arranged in the annular cylinder. According to the nucleic acid extractor provided by the utility model, when the culture tube is held by hand and lifted upwards, the culture tube synchronously pulls the support frame to move upwards, so that heated water in the vertical cylinder can provide heat preservation for the culture tube separated from the groove, and meanwhile, cold water in the liquid storage cavity enters the vertical cylinder to be neutralized so as to reduce the water temperature in the vertical cylinder and cool the heat-preserved water in the vertical cylinder; the heat preservation device is used for shortening the heat preservation time of a culture tube, and during the period, a culture medium cannot be damaged after the temperature in a vertical cylinder is neutralized until the culture tube can adapt to the environment temperature, so that the reaction time of the culture medium adapting to the environment temperature is provided, and inactivation of a cell sample caused by overlarge temperature change is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid collection and extraction, in particular to a culture medium replacement device for immune cell proliferation. Background Art

[0002] Immune cell proliferation culture tubes are also a type of culture vessel and are suitable for culturing fewer cells. They are undoubtedly a good choice for some immune cells that are difficult to proliferate.

[0003] According to the publication (announcement) number: CN218620864U, the publication (announcement) date: 2023-03-14, a cell culture box that is convenient for replacing culture medium is disclosed.

[0004] In the prior art including the above-mentioned patents, during the proliferation culture of immune cells using culture tubes, in order to directly replace the culture medium, a new culture tube loaded with the culture medium is usually directly replaced to achieve the purpose of efficient culture. However, for the culture medium that needs to be preheated, the culture tube is easily caused to lose temperature when it is in a room temperature environment, affecting the accuracy of the control treatment of the culture medium. There is a water bath method to keep the culture tube warm, but when the culture tube is removed from the water bath rack, it will still cause excessive temperature changes due to sudden contact with the environment, which reduces the culture efficiency of the culture medium and easily causes the immune cells to be inactivated, thereby affecting the culture work of the culture medium. Utility Model Content

[0005] The purpose of the utility model is to provide a device for replacing culture medium for proliferation of immune cells, aiming to solve the above-mentioned problems.

[0006] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0007] A device for replacing culture medium for proliferation of immune cells, comprising a water bath having a plurality of grooves, and a support frame movable in the grooves and used to support culture tubes, wherein the support frame comprises a ring tube and a vertical tube arranged in an annular manner outside the culture tubes;

[0008] A liquid storage cavity is provided in the annular cylinder, and the supporting frame moves upward synchronously with the culture tube, and the liquid storage cavity drains cold water into the multiple vertical cylinders.

[0009] Preferably, a support frame is fixedly installed in the groove, and a partition column slidably assembled with the vertical cylinder is fixedly installed on the support frame.

[0010] Preferably, the partition column divides the vertical cylinder to form a heat conducting cavity and a heat receiving cavity, and the heat conducting cavity transfers heat to the heat receiving cavity and acts on the culture tube.

[0011] Preferably, a flap is hingedly provided at the end of the partition column, and the heat conduction cavity drives the flap to deflect when heated.

[0012] Preferably, in the upward state, the liquid storage chamber drives the flap to deflect by itself, and allows cold water to mix with the heat conduction chamber and the heat receiving chamber.

[0013] In the above technical solution, the utility model provides a medium replacement device for immune cell proliferation, which has the following beneficial effects: the warm water in the groove can directly transfer heat to the culture tube through the space between each two vertical cylinders, and the water in the vertical cylinder is first transferred heat by the warm water and then transferred to the culture tube, so that the entire culture tube has a buffer space when heated, so that the temperature distribution of the culture tube is more balanced when heated, and when the culture tube is lifted by hand, the culture tube synchronously pulls the support frame upward, so that the heated water in the vertical cylinder can provide insulation for the culture tube that has left the groove, and at the same time the cold water in the liquid storage chamber enters The water is then cooled and cooled in the vertical cylinder, which is used to shorten the insulation time of the culture tube. During this period, the temperature in the vertical cylinder is neutralized and the culture medium will not be damaged until the culture tube can adapt to the ambient temperature, thereby providing the culture medium with reaction time to adapt to the ambient temperature and avoiding inactivation of cell samples due to excessive temperature changes. This makes the culture tube safer during the process of taking and placing, and ensures the integrity of the sample. At this time, the culture tube is separated from the support frame while continuing to lift it up, so that the support frame can slide down and reset itself due to gravity, making it convenient to place the culture tube again. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0015] Figure 1 A schematic diagram of a water bath box, an assembled support frame, and a culture tube in the automated equipment provided by an embodiment of the present utility model;

[0016] Figure 2 A schematic side cross-sectional view of a support frame and a culture tube provided in an embodiment of the present utility model;

[0017] Figure 3 for Figure 2 A magnified schematic diagram of point A;

[0018] Figure 4 This is a schematic diagram of the exploded structure of the culture tube, support frame and support frame provided in an embodiment of the present utility model.

[0019] Description of reference numerals:

[0020] 1. Water bath; 11. Culture tube; 2. Support rack; 21. Ring cylinder; 211. Liquid storage chamber; 22. Vertical cylinder; 221. Heat transfer chamber; 222. Heating chamber; 3. Support rack; 31. Partition column; 32. Flip plate. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] like Figure 1-4 As shown, a medium replacement device for immune cell proliferation includes a water bath 1 with multiple grooves, and a support frame 2 that moves within the grooves and is used to support a culture tube 11. The support frame 2 is divided into a ring tube 21 and a vertical tube 22 arranged in an annular manner outside the culture tube 11.

[0023] A liquid storage chamber 211 is provided in the annular cylinder 21 . The support frame 2 moves upward synchronously with the culture tube 11 , and the liquid storage chamber 211 drains cold water into the multiple vertical cylinders 22 .

[0024] Specifically, warm water is stored in the groove. The groove setting method and the water temperature constant method are both existing technologies and will not be described in detail here. The ring tube 21 is higher than the water bath box 1 so that the water in the liquid storage chamber 211 can cool down naturally.

[0025] Furthermore, a rubber ring is fixedly installed on the inner ring of the ring tube 21 so that the culture tube 11 is squeezed by the rubber ring when inserted into the support frame 2, thereby achieving an anti-slip effect.

[0026] The warm water in the groove can directly transfer heat to the culture tube 11 between each two vertical cylinders 22, and the water in the vertical cylinder 22 is first transferred heat by the warm water and then transferred to the culture tube 11, so that the entire culture tube 11 has a buffer space when heated, so that the temperature distribution of the culture tube 11 is more balanced when heated. When the culture tube 11 is lifted by hand, the culture tube 11 pulls the support frame 2 upward synchronously, so that the heated water in the vertical cylinder 22 can provide heat insulation for the culture tube 11 that is out of the groove, and at the same time, the cold water in the liquid storage chamber 211 enters the vertical cylinder 22 to neutralize it, so as to reduce the water in the vertical cylinder 22. Temperature, so that the insulation water in the vertical cylinder 22 is cooled, which is used to shorten the insulation time of the culture tube 11. During this period, the temperature in the vertical cylinder 22 is neutralized and the culture medium will not be damaged until the culture tube 11 can adapt to the ambient temperature, thereby providing the culture medium with reaction time to adapt to the ambient temperature, avoiding excessive temperature changes to cause inactivation of cell samples, making the culture tube 11 safer during the process of taking and placing, and ensuring the integrity of the sample. At this time, the culture tube 11 is still lifted up to separate it from the support frame 2, so that the support frame 2 slides down and resets itself due to gravity, which is convenient for the culture tube 11 to be placed again.

[0027] As an embodiment further provided by the present invention, a support frame 3 is fixedly installed in the groove, and a partition column 31 is fixedly installed on the support frame 3 and is slidably assembled with the vertical cylinder 22.

[0028] Specifically, the number of the partition columns 31 corresponds to the number of the vertical cylinders 22, and a support block that supports the bottom of the culture tube 11 is fixedly installed on the support frame 3 to ensure the stability of the placement of the culture tube 11.

[0029] As the support frame 2 is lifted by the culture tube 11 and moves upward synchronously, the plurality of partition columns 31 provide guide limits for the vertical cylinder 22 , so that the support frame 2 can wrap the culture tube 11 and move upward more stably.

[0030] As another embodiment further provided by the present invention, the partition column 31 divides the vertical cylinder 22 to form a heat conducting cavity 221 and a heat receiving cavity 222 . The heat conducting cavity 221 transfers heat to the heat receiving cavity 222 and acts on the culture tube 11 .

[0031] Specifically, the heat conducting cavity 221 is located outside the heat receiving cavity 222 , and cold water is provided in both.

[0032] The heat is transferred to the heat conduction cavity 221 through the groove, and then transferred to the heating cavity 222 after being heated, so as to reduce the heating rate of the side wall of the culture tube 11 so that the heating condition can be directly contacted with the warm water on the side wall of the culture tube 11 to be neutralized, thereby avoiding damage to the culture tube 11 caused by temperature changes.

[0033] As another embodiment further provided by the present invention, a flap 32 is hingedly provided at the end of the partition column 31 , and the heat-conducting cavity 221 drives the flap 32 to deflect when heated.

[0034] Specifically, the flap 32 remains horizontal in a default state, and a torsion spring is provided at the hinged position.

[0035] When the temperature in the heat conduction cavity 221 rises, the warm water inside it causes the heat conduction cavity 221 to expand and deflect the flip plate 32, so that the cold water in the liquid storage cavity 211 mixes into the heat conduction cavity 221 and the heating cavity 222 through the gap in the deflected flip plate 32, so that the cold water can cool down the warm water in the vertical cylinder 22, thereby preventing the temperature change from damaging the side wall of the culture tube 11 being heated.

[0036] As another embodiment further provided by the present invention, in the upward state, the liquid storage chamber 211 automatically drives the flap 32 to deflect, and causes cold water to mix with the heat conduction chamber 221 and the heat receiving chamber 222 .

[0037] Specifically, the partition column 31 and the bottom of the vertical cylinder 22 are slidably arranged, and a rubber ring is fixedly installed at a penetrating position to provide sealing for the vertical cylinder 22 to prevent warm water in the groove from mixing into the vertical cylinder 22.

[0038] During the upward movement of the support rack 2, the water in the heat conduction chamber 221 and the heat receiving chamber 222 is actively compressed by the flap 32, and the flap 32 is driven to deflect by the water pressure, so that the cold water in the liquid storage chamber 211 can completely enter the two chambers, so that the neutralized cooling water can keep the culture tube 11 warm, and can also ensure the separation time of the culture tube 11, making the culture tube 11 safer during transportation and use.

[0039] Working principle: The warm water in the groove can transfer heat directly to the culture tube 11 between every two vertical cylinders 22, and the water in the vertical cylinder 22 is first transferred heat by the warm water and then transferred to the culture tube 11, so that the entire culture tube 11 has a buffer space when heated, and when the culture tube 11 is lifted up by hand, the culture tube 11 simultaneously pulls the support frame 2 upward, so that the heated water in the vertical cylinder 22 can provide insulation for the culture tube 11 that has separated from the groove, and at the same time, the cold water in the liquid storage chamber 211 enters the vertical cylinder 22 for neutralization, so as to reduce the water temperature in the vertical cylinder 22 and cool the insulation water in the vertical cylinder 22, so as to shorten the insulation time of the culture tube 11, and during this period, the temperature in the vertical cylinder 22 is neutralized and will not cause damage to the culture medium until the culture tube 11 can adapt to the ambient temperature. At this time, the culture tube 11 is separated from the support frame 2 during the process of continuing to lift it up, so that the support frame 2 slides down and resets itself due to gravity.

[0040] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A medium replacement device for immune cell proliferation, comprising a water bath box (1) with a plurality of grooves, characterized in that: It also includes a support frame (2) that moves in the groove and is used to support the culture tube (11), and the support frame (2) is divided into a ring cylinder (21) and a vertical cylinder (22) that is arranged in an annular shape outside the culture tube (11); A liquid storage cavity (211) is provided in the annular cylinder (21), and the supporting frame (2) moves upward synchronously with the culture tube (11), and the liquid storage cavity (211) drains cold water into the multiple vertical cylinders (22).

2. The device for replacing culture medium for immune cell proliferation according to claim 1, characterized in that: A support frame (3) is fixedly installed in the groove, and a partition column (31) is fixedly installed on the support frame (3) and is slidably assembled with the vertical cylinder (22).

3. The device for replacing culture medium for immune cell proliferation according to claim 2, characterized in that: The partition column (31) divides the vertical cylinder (22) to form a heat-conducting cavity (221) and a heat-receiving cavity (222). The heat-conducting cavity (221) transfers heat to the heat-receiving cavity (222) and acts on the culture tube (11).

4. The device for replacing culture medium for immune cell proliferation according to claim 3, characterized in that: A flap (32) is hingedly provided at the end of the partition column (31), and in a heated state, the heat conduction cavity (221) drives the flap (32) to deflect.

5. The device for replacing culture medium for immune cell proliferation according to claim 3, characterized in that: In the upward state, the liquid storage chamber (211) automatically drives the flap (32) to deflect, and causes cold water to mix with the heat conducting chamber (221) and the heat receiving chamber (222).

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