Cooling device for protein chromatographic purification

Abstract

The utility model belongs to the technical field of cooling devices, and particularly relates to a cooling device for protein chromatographic purification, which comprises a protein chromatographic column and a cooling tank sleeved outside the protein chromatographic column, the side wall of the cooling tank is fixedly connected with fixing lugs, the upper end and the lower end of the cooling tank are fixedly provided with flange plates, and the flange plates are fixedly connected with the fixing lugs. A sealing plate is sealed at the upper part of the cooling tank, a protein chromatographic column is loaded on the sealing plate in a re-sealing manner, a flange water outlet pipe is communicated with the side wall of the cooling tank, and a flange water inlet pipe is communicated with the side wall of the cooling tank. According to the utility model, one to a plurality of protein chromatographic columns can be adapted to meet different requirements, the fixing lugs are fixed through screw threads of the bolt holes, the stability is ensured, the reasonable height design of the water inlet and outlet pipes and the rotating blades promote the circulation of cooling liquid, the cooling efficiency is improved, and the temperature and liquid level sensor is matched with the processor to realize automatic control; a stable cooling environment is ensured, protein chromatography purification is facilitated, cooling liquid can be conveniently emptied through a communicating pipe, and maintenance is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of cooling devices, in particular to a cooling device for protein chromatography purification. Background Art

[0002] In the fields of biopharmaceuticals and biotechnology, protein chromatography purification is a key technical means for separating and purifying target proteins. During protein chromatography, temperature has a significant impact on the activity and stability of proteins.

[0003] Typically, to ensure that proteins maintain good activity and stability during purification, protein chromatography columns need to be maintained at a specific low temperature. Traditional cooling methods may have some shortcomings, such as:

[0004] Lack of dedicated cooling equipment: In some cases, the protein chromatography column may simply be placed in a normal refrigerated environment. However, this method makes it difficult to accurately control the temperature, and the temperature stability is poor and is easily affected by changes in the external ambient temperature.

[0005] Low cooling efficiency: If conventional cooling methods such as natural cooling or simple air cooling are used, the cooling speed is slow and cannot meet the requirements of efficient protein chromatography purification. Especially in large-scale production or when rapid sample processing is required, inefficient cooling will extend the production cycle and reduce production efficiency.

[0006] In summary, in order to meet the strict temperature requirements during protein chromatography purification, improve cooling efficiency and temperature stability, and achieve automatic control, it is of great practical significance to develop a cooling device specifically for protein chromatography purification. Utility Model Content

[0007] (1) Technical problems solved

[0008] In view of the deficiencies of the prior art, the present invention provides a cooling device for protein chromatography purification, which solves the problems raised in the above-mentioned background technology.

[0009] (2) Technical solution

[0010] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions:

[0011] A cooling device for protein chromatography purification comprises a protein chromatography column and a cooling tank sleeved on the outside of the protein chromatography column, wherein a fixing ear is fixedly connected to the side wall of the cooling tank, flanges are fixedly provided at the upper and lower ends of the cooling tank, the upper portion of the cooling tank is sealed with a sealing plate, a protein chromatography column is sealed and loaded in the sealing plate, a flange water outlet pipe is connected to the side wall of the cooling tank, a flange water inlet pipe is connected to the side wall of the cooling tank, a solenoid valve is provided at one point of the flange water inlet pipe, a solenoid valve is provided at one point of the flange water outlet pipe, a connecting pipe is provided at the lowest point of the cooling tank, a solenoid valve is provided at one point of the connecting pipe, a rotating blade is installed inside the cooling tank at the connection point of the flange water inlet pipe, and a temperature sensor and a liquid level sensor are installed inside the cooling tank.

[0012] Furthermore, the protein chromatography columns are provided with one to several columns, which are adapted to the sealing plate.

[0013] Furthermore, bolt holes are provided on the side walls of the fixing ears for threaded fixing.

[0014] Furthermore, the designed height of the flange water inlet pipe is lower than the designed height of the flange water outlet pipe.

[0015] Furthermore, the connecting pipe is used to drain the coolant in the cooling tank.

[0016] Furthermore, the temperature sensor and the liquid level sensor are connected to a processor, and the processor controls the switch of the solenoid valve.

[0017] (3) Beneficial effects

[0018] Compared with the prior art, the present invention provides a cooling device for protein chromatography purification, which has the following beneficial effects:

[0019] The utility model can be adapted to one to several protein chromatography columns to meet different needs. The fixing ears are fixed by screw threads through bolt holes to ensure stability. The reasonable height design of the inlet and outlet water pipes and the rotating blades promote the circulation of the coolant and improve the cooling efficiency. The temperature and liquid level sensors cooperate with the processor to realize automatic control and ensure a stable cooling environment, which is beneficial to protein chromatography purification. The connecting pipe is convenient for emptying the coolant and convenient for maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the three-dimensional structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0022] Figure 3 This is a schematic diagram of the system structure of the utility model.

[0023] In the figure: 1. Cooling tank; 2. Fixing ear; 3. Flange; 4. Sealing plate; 5. Protein chromatography column; 6. Flange outlet pipe; 7. Flange inlet pipe; 8. Rotating blade; 9. Temperature sensor; 10. Liquid level sensor; 11. Connecting pipe. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example

[0026] like Figure 1-3 As shown, a cooling device for protein chromatography purification proposed in one embodiment of the present invention includes a protein chromatography column 5 and a cooling tank 1 sleeved on the outside of the protein chromatography column 5.

[0027] A fixing ear 2 is fixedly connected to the side wall of the cooling tank 1, a flange 3 is fixed at the upper and lower ends, a sealing plate 4 is sealed on the upper part, a flange water outlet pipe 6 and a flange water inlet pipe 7 are connected to the side wall, a connecting pipe 11 is provided at the lowest point, and a rotating blade 8, a temperature sensor 9 and a liquid level sensor 10 are installed inside.

[0028] Function: Serves as the core container of the entire cooling device, containing the coolant and providing a cooling environment for the protein chromatography column 5. The fixing ears 2 secure the cooling tank 1, the flange 3 facilitates connection to other components, the sealing plate 4 ensures the upper portion of the cooling tank 1 is sealed and can accommodate the protein chromatography column 5. The inlet and outlet pipes circulate the coolant, the connecting pipe 11 drains the coolant, the rotating blades 8 facilitate coolant flow, and the temperature sensor 9 and liquid level sensor 10 monitor the internal environment.

[0029] Bolt holes are provided on the side walls of the fixing ears 2.

[0030] Function: The cooling tank 1 can be threadedly fixed in a suitable position using bolts through the bolt holes.

[0031] The flanges 3 are fixed to the upper and lower ends of the cooling tank 1 .

[0032] Function: To facilitate connection with other equipment or components and ensure the sealing and stability of the cooling tank 1.

[0033] The sealing plate 4 seals the upper portion of the cooling tank 1 and is adapted to accommodate the protein chromatography column 5 .

[0034] Function: Prevent leakage of cooling liquid and provide a stable placement environment for the protein chromatography column 5 to ensure cooling effect.

[0035] One to several protein chromatography columns 5 are provided and adapted to the sealing plate 4 .

[0036] Function: It is the core component of protein chromatography purification, placed in the cooling tank 1, and performs protein chromatography purification in a cooling environment.

[0037] The flange water outlet pipe 6 is connected to the side wall of the cooling tank 1 .

[0038] Function: Used to discharge the coolant in the cooling tank 1 to achieve the circulation and renewal of the coolant.

[0039] The flange water inlet pipe 7 is connected to the side wall of the cooling tank 1, and a solenoid valve is provided at one place. The design height is lower than the flange water outlet pipe 6, and a rotating blade 8 is installed inside the cooling tank 1 at the connection point.

[0040] Function: Used to inject coolant into the cooling tank 1. The solenoid valve controls the inflow of coolant. The rotating blades 8 promote better flow and distribution of coolant when it flows in. The height design is conducive to the reasonable circulation of coolant.

[0041] The rotating blades 8 are installed inside the cooling tank 1 at the connection of the flange water inlet pipe 7 .

[0042] Function: When the coolant flows in from the flange water inlet pipe 7, the rotating blades 8 rotate with the water flow, making the coolant flow and distribute more evenly in the cooling tank 1, thereby improving the cooling effect.

[0043] The temperature sensor 9 is installed inside the cooling tank 1 .

[0044] Function: Real-time monitoring of the temperature in cooling tank 1 to provide data support for temperature control.

[0045] The liquid level sensor 10 is installed inside the cooling tank 1 .

[0046] Function: Real-time monitoring of the coolant level in the cooling tank 1 to provide a basis for the replenishment and control of the coolant.

[0047] Connecting pipe 11

[0048] Structural design features: It is set at the lowest point of the cooling tank 1 and a solenoid valve is provided.

[0049] Function: Used to drain the coolant in the cooling tank 1, to facilitate equipment maintenance and coolant replacement, and to drain when not needed.

[0050] The working principle of the protein chromatography purification cooling device is as follows: first, the coolant enters the cooling tank 1 through the flange water inlet pipe 7, and the solenoid valve at one end of the flange water inlet pipe 7 controls the inflow of the coolant. When the coolant enters, the rotating blade 8 installed at its connection inside the cooling tank 1 will rotate with the flow of the coolant, so that the coolant is more evenly distributed in the cooling tank 1. The temperature sensor 9 installed in the cooling tank 1 monitors the temperature in real time, and the liquid level sensor 10 monitors the coolant level in real time. When the temperature is higher than the set value, the flow rate or circulation speed of the coolant can be adjusted by controlling the relevant solenoid valve by the processor to adjust the temperature. When the coolant needs to be emptied, the solenoid valve at the connecting pipe 11 can be opened, and the coolant is discharged from the connecting pipe 11. At the same time, the flange water outlet pipe 6 is used for the circulation outflow of the coolant, and the solenoid valve at one end can also be controlled as needed to achieve reasonable circulation of the coolant in the cooling tank 1, thereby providing a stable cooling environment for the protein chromatography column 5 that is sleeved in the cooling tank 1 and sealed and loaded by the sealing plate 4.

[0051] like Figure 1 As shown, in some embodiments, the protein chromatography columns 5 are provided with one or more columns, adapted to the sealing plate 4; this design is to increase the applicability and flexibility of the cooling device. In the actual protein chromatography purification process, it may be necessary to use a single or multiple protein chromatography columns 5 according to different experimental or production requirements.

[0052] like Figure 1 As shown, in some embodiments, the sidewalls of the fixing ears 2 are provided with bolt holes for threaded fixing. Bolt holes in the fixing ears 2 are a common and effective fixing method. By inserting bolts through the bolt holes, the cooling device can be connected to other equipment or supporting structures. When the bolts are tightened, friction and clamping force are generated, firmly securing the cooling device in place.

[0053] like Figure 2 As shown, in some embodiments, the design height of the flange water inlet pipe 7 is lower than the design height of the flange water outlet pipe 6; the design of this height difference is based on the principle of fluid mechanics. The coolant will naturally flow from high to low under the action of gravity. The flange water inlet pipe 7 is located at a lower position, so that the coolant can enter the cooling tank 1 more easily under the action of its own gravity and external pressure, such as the pressure of a pump. The flange water outlet pipe 6 is located at a higher position. When the coolant in the cooling tank 1 reaches a certain height, the higher position of the water outlet pipe can make the coolant flow out more smoothly, thereby realizing the natural circulation of the coolant in the cooling tank 1.

[0054] like Figure 2As shown, in some embodiments, the connecting pipe 11 is used to drain the coolant from the cooling tank 1. During the protein chromatography purification process, the coolant may need to be replaced regularly to ensure the cooling performance and cleanliness of the coolant. In addition, if the cooling device needs maintenance or repair, draining the coolant is also a necessary operation.

[0055] like Figure 3 As shown, in some embodiments, the temperature sensor 9 and liquid level sensor 10 are connected to a processor, which controls the opening and closing of the solenoid valve. The temperature sensor 9 and liquid level sensor 10 are connected to the processor via suitable circuit connections. The temperature sensor 9 converts temperature information within the cooling tank 1 into an electrical signal and transmits it to the processor. The liquid level sensor 10 converts coolant level information into an electrical signal and transmits it to the processor. After receiving these signals, the processor can analyze and process them.

[0056] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A cooling device for protein chromatography purification, comprising a protein chromatography column (5) and a cooling tank (1) sleeved on the outside of the protein chromatography column (5), characterized in that: A fixing ear (2) is fixedly connected to the side wall of the cooling tank (1), a flange (3) is fixedly provided at the upper and lower ends of the cooling tank (1), the upper part of the cooling tank (1) is sealed with a sealing plate (4), a protein chromatography column (5) is sealed in the sealing plate (4), a flange outlet pipe (6) is connected to the side wall of the cooling tank (1), a flange inlet pipe (7) is connected to the side wall of the cooling tank (1), a solenoid valve is provided at one point of the flange inlet pipe (7), a solenoid valve is provided at one point of the flange outlet pipe (6), a connecting pipe (11) is provided at the lowest point of the cooling tank (1), a solenoid valve is provided at one point of the connecting pipe (11), a rotating blade (8) is installed inside the cooling tank (1) at the connection point of the flange inlet pipe (7), and a temperature sensor (9) and a liquid level sensor (10) are installed inside the cooling tank (1).

2. A cooling device for protein chromatography purification according to claim 1, characterized in that: The protein chromatography columns (5) are provided with one to several columns, and are adapted to the sealing plate (4).

3. A cooling device for protein chromatography purification according to claim 1, characterized in that: Bolt holes are provided on the side walls of the fixing ears (2) for threaded fixing.

4. A cooling device for protein chromatography purification according to claim 1, characterized in that: The designed height of the flange water inlet pipe (7) is lower than the designed height of the flange water outlet pipe (6).

5. A cooling device for protein chromatography purification according to claim 1, characterized in that: The connecting pipe (11) is used to drain the coolant in the cooling tank (1).

6. A cooling device for protein chromatography purification according to claim 1, characterized in that: The temperature sensor (9) and the liquid level sensor (10) are connected to a processor, and the processor controls the switch of the solenoid valve.

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