Device for simulating transmembrane contact substance in living body

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By designing devices including simulator I and simulator II, the problem of lack of experimental equipment for simulating transmembrane contact of substances in organisms in the existing technology has been solved, and the simulation and experiment of transmembrane transport processes in organisms have been realized, with efficient and low-cost experimental capabilities.

CN223304424UActive Publication Date: 2025-09-05KUNMING UNIV OF SCI & TECH

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Patent Information

Application Number: CN202422484160.X
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-10-15
Publication Date: 2025-09-05
Estimated Expiration: 2034-10-15

AI Technical Summary

Technical Problem

The existing technology lacks experimental equipment to simulate the transmembrane contact of substances in organisms and cannot effectively simulate the transmembrane transport process in organisms.

Method used

A device was designed, which included simulator I, simulator II, a flow regulating pump, a double-hole piston, a main tank, an insulated box, simulator I, a thermos, a hose, a liquid storage bottle and other components. The temperature was controlled by the hose connection and the insulated box to simulate the transmembrane transport process in organisms.

Benefits of technology

It realizes the simulation of transmembrane flow and flow through contact substances in organisms. It is simple to operate, low-cost, highly automated, and can conduct experiments under a constant temperature environment. It is suitable for simulating the internal environments of different organisms.

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Abstract

The utility model discloses a device for simulating a transmembrane contact substance in a living body. Comprising a simulator II, a flow adjusting pump, a double-hole piston, a main tank body, a heat preservation box, a simulator I, a heat preservation bottle, a hose, a liquid storage bottle, an upper closing pipe, a cylindrical liquid storage bottle, a circular biological membrane, a lower closing pipe, a rectangular-column-shaped liquid storage bottle, a rectangular-column-shaped pipe and a rectangular biological membrane. The device is easy to operate, low in cost, high in automation degree, capable of being operated by one person, high in efficiency and capable of achieving simulation of transmembrane flowing and flowing contact with a certain substance in a living body at the same time, and a transverse comparison can be conveniently made; the main body parts of the device are all arranged in the heat preservation box, the temperature in the main tank body and the temperature in the liquid storage bottle can be effectively kept consistent, the temperature of the heat preservation box can be adjusted according to needs, and the environmental condition in a living body is simulated to the maximum extent.

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Description

Technical Field

[0001] The utility model belongs to the technical field of experimental instruments, and in particular relates to a device for simulating transmembrane contact of substances in a living body. Background Art

[0002] Transmembrane transport plays a crucial role in living organisms. Transmembrane transport in organisms can be categorized into two modes: active transport and passive transport. Active transport refers to the process by which the cell membrane, through its own energy-consuming processes, transports substances across the membrane against the concentration gradient with the help of specialized proteins. Passive transport, on the other hand, occurs when molecules follow the concentration gradient, from the high-concentration side to the low-concentration side, and the energy required for transport comes from the potential energy contained in the high concentration. Passive transport includes simple diffusion and facilitated diffusion. Currently, there are no experimental devices on the market that can simulate the transmembrane contact of a substance within a living organism. Therefore, the development of a device that simulates transmembrane contact of substances within a living organism is highly desirable. Utility Model Content

[0003] The purpose of the utility model is to provide a device for simulating transmembrane contact of substances in a living body.

[0004] The purpose of the utility model is achieved in this way, including a simulator II, a flow regulating pump, a double-hole piston, a main tank body, an insulation box, a simulator I, a thermos, a hose, a liquid storage bottle, an upper closed tube, a cylindrical liquid storage bottle, a circular biofilm, a lower closed tube, a rectangular prism-shaped liquid storage bottle, a rectangular prism-shaped tube and a rectangular biofilm, the top of the main tank body is provided with an opening, and the opening is equipped with a double-hole piston, two through holes of the double-hole piston, one through hole, a flow regulating pump, a simulator II and a liquid storage bottle are connected in sequence through a hose, the other through hole, a flow regulating pump, a simulator I and a liquid storage bottle are connected in sequence through a hose, and the ends of the hoses in the main tank body are all located below the liquid level in the main tank body, the simulator II and the tube body connected to the simulator II are located in a thermos, the simulator I and the tube body connected to the simulator I are located in another thermos, the simulator II, the flow regulating pump, the double-hole piston, the main tank body, the simulator I and the thermos are all located in the insulation box;

[0005] The simulator I comprises a lower closed tube, a circular biofilm, a cylindrical liquid storage bottle, a circular biofilm, and an upper closed tube, which are sequentially installed from bottom to top. The lower closed tube and the upper closed tube are buckled and sealed. The two circular biofilms and the cylindrical liquid storage bottle are located in a space formed by the buckling of the lower closed tube and the upper closed tube. The outer sides of the lower closed tube and the upper closed tube are provided with connecting tubes, which are connected to the hose 8.

[0006] The simulator II1 includes a rectangular prism-shaped liquid storage bottle, a rectangular biofilm and a rectangular prism-shaped tube installed in sequence from left to right. The side extension of the rectangular prism-shaped tube and the side of the rectangular prism-shaped liquid storage bottle are buckled and sealed. The rectangular biofilm is located in the space formed by the buckling of the rectangular prism-shaped tube and the rectangular prism-shaped liquid storage bottle. Connecting tubes are respectively provided at the top and bottom of the rectangular prism-shaped tube, and the connecting tubes are connected to the hose.

[0007] Preferably, the incubator is provided with a temperature control device, which may use a temperature regulating control device well known to those skilled in the art to maintain a constant temperature inside the incubator to simulate the actual temperature inside the organism.

[0008] Preferably, the thermos bottle is filled with insulation liquid to maintain the same temperature environment as the main tank.

[0009] Preferably, the upper closed tube contacts the circular biofilm, and the contact surface of the upper closed tube is mesh-shaped.

[0010] Preferably, both the upper and lower surfaces of the cylindrical liquid storage bottle are in contact with the corresponding circular biofilm, and the upper and lower contact surfaces of the cylindrical liquid storage bottle are both mesh-shaped.

[0011] Preferably, the lower closed tube contacts the circular biofilm, and the contact surface of the lower closed tube is mesh-shaped.

[0012] Preferably, the rectangular parallelepiped liquid storage bottle contacts the rectangular biofilm, and the contact surface of the rectangular parallelepiped liquid storage bottle is mesh-shaped.

[0013] Preferably, the rectangular parallelepiped tube contacts the rectangular biofilm, and the contact surface of the rectangular parallelepiped tube is mesh-shaped.

[0014] The beneficial effects of the present invention are as follows: the present invention is simple to operate, the components are easy to assemble, the cost is low, the automation is high, the operation can be done by one person, and the efficiency is high. It can simultaneously realize the simulation of transmembrane flow and flow through contact with a certain substance in a biological body, which is convenient for making a horizontal comparison; the main parts of the present invention are all placed in an insulated box, which can effectively keep the temperature in the main tank body and the liquid storage bottle consistent, and the insulated box can adjust the temperature as needed to maximize the simulation of the environmental conditions in the biological body. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural diagram of the utility model;

[0016] Figure 2 This is the structural diagram of simulator I;

[0017] Figure 3 It is the structural diagram of simulator II;

[0018] In the figure: 1-Simulator II, 2-Flow regulating pump, 3-Double-hole piston, 4-Main tank, 5-Insulation box, 6-Simulator I, 7-Thermos flask, 8-Hose, 9-Liquid storage bottle, 10-Upper closed tube, 11-Cylindrical liquid storage bottle, 12-Circular biofilm, 13-Lower closed tube, 14-Rectangular prism-shaped liquid storage bottle, 15-Rectangular prism-shaped tube, 16-Rectangular biofilm. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with the embodiments and drawings, but is not intended to limit the present invention in any way. Any changes or substitutions made based on the teachings of the present invention fall within the scope of protection of the present invention.

[0020] Example 1

[0021] As attached Figures 1 to 3 As shown, the device for simulating transmembrane contact of substances in a living body in this embodiment includes a simulator II 1, a flow regulating pump 2, a double-hole piston 3, a main tank body 4, an insulation box 5, a simulator I 6, a thermos flask 7, a hose 8, a liquid storage bottle 9, an upper closed tube 10, a cylindrical liquid storage bottle 11, a circular biofilm 12, a lower closed tube 13, a rectangular prism-shaped liquid storage bottle 14, a rectangular prism-shaped tube 15 and a rectangular biofilm 16. The top of the main tank body 4 is provided with an opening, and the opening is equipped with a double-hole piston 3. The two through holes of the double-hole piston 3, one of which is a through hole, a flow regulating pump 2, Simulator II 1 and a liquid storage bottle 9 are sequentially connected via a hose 8. Another through hole, a flow regulating pump 2, simulator I 6, and a liquid storage bottle 9 are sequentially connected via a hose 8. The ends of the hose 8 in the main tank 4 are all located below the liquid level in the main tank 4. The simulator II 1 and the tube connected to the simulator II 1 are located in a thermos flask 7. The simulator I 6 and the tube connected to the simulator I 6 are located in another thermos flask 7. The simulator II 1, the flow regulating pump 2, the double-hole piston 3, the main tank 4, the simulator I 6, and the thermos flask 7 are all located in an insulation box 5.

[0022] The simulator I 6 includes a lower closed tube 13, a circular biofilm 12, a cylindrical liquid storage bottle 11, a circular biofilm 12, and an upper closed tube 10, which are sequentially installed from bottom to top. The lower closed tube 13 and the upper closed tube 10 are buckled and sealed. The two circular biofilms 12 and the cylindrical liquid storage bottle 11 are located in the space formed by the buckling of the lower closed tube 13 and the upper closed tube 10. The outer sides of the lower closed tube 13 and the upper closed tube 10 are both provided with connecting pipes, which are connected to the hose 8.

[0023] The simulator II 1 includes a rectangular prism-shaped liquid storage bottle 14, a rectangular biofilm 16, and a rectangular prism-shaped tube 15, which are installed in sequence from left to right. The side extension of the rectangular prism-shaped tube 15 is buckled and sealed with the side of the rectangular prism-shaped liquid storage bottle 14. The rectangular biofilm 16 is located in the space formed by the buckling of the rectangular prism-shaped tube 15 and the rectangular prism-shaped liquid storage bottle 14. Connecting pipes are respectively provided at the top and bottom of the rectangular prism-shaped tube 15, and the connecting pipes are connected to the hose 8.

[0024] The device of this embodiment was used to conduct a non-biological dissolution flow experiment simulating an intrapulmonary environment. The liquid contained in the main tank body 4 was pulmonary endothelial fluid, the liquid contained in the cylindrical liquid storage bottle 11 was a 1 mg / mL BaSO4 buffer solution, the liquid contained in the rectangular liquid storage bottle 14 was a 1 mg / mL BaSO4 buffer solution, the circular biomembrane 12 specifically selected a 3.5 kDa cellulose ester symmetric membrane, and the rectangular biomembrane 16 specifically selected a 3.5 kDa cellulose ester symmetric membrane. The flow regulating pump 2 was set to a speed of 60 μL / min, and the temperature of the insulated box 5 was set to 37°C. At the beginning of the experiment, under the action of the flow regulating pump 2, the liquid in the main tank body 4 passed through the cylindrical liquid storage bottle 11 and the rectangular liquid storage bottle 14, and some substances penetrated the biomembrane and merged into the flowing liquid, and flowed together into the liquid storage bottle 9.

[0025] Example 2

[0026] The device for simulating transmembrane contact of substances in a living body in this embodiment is based on embodiment 1. Preferably, the thermal insulation box 5 is provided with a temperature control device; the thermal insulation bottle 7 contains thermal insulation liquid; the upper closed tube 10 is in contact with the circular biofilm 12, and the contact surface of the upper closed tube 10 is mesh-shaped; the upper and lower surfaces of the cylindrical liquid storage bottle 11 are in contact with the corresponding circular biofilm 12, and the upper and lower contact surfaces of the cylindrical liquid storage bottle 11 are mesh-shaped; the lower closed tube 13 is in contact with the circular biofilm 12, and the contact surface of the lower closed tube 13 is mesh-shaped; the rectangular prism-shaped liquid storage bottle 14 is in contact with the rectangular biofilm 16, and the contact surface of the rectangular prism-shaped liquid storage bottle 14 is mesh-shaped; the rectangular prism-shaped tube 15 is in contact with the rectangular biofilm 16, and the contact surface of the rectangular prism-shaped tube 15 is mesh-shaped.

[0027] The device of this embodiment was used to conduct a non-biological dissolution flow experiment simulating an intracellular environment. The liquid contained in the main tank body 4 was phagolysosomal fluid, the liquid contained in the cylindrical liquid storage bottle 11 was a 1 mg / mL BaSO4 buffer solution, the liquid contained in the rectangular liquid storage bottle 14 was a 1 mg / mL BaSO4 buffer solution, the circular biomembrane 12 specifically selected a 3.5 kDa cellulose ester symmetric membrane, and the rectangular biomembrane 16 specifically selected a 3.5 kDa cellulose ester symmetric membrane. The speed of the flow regulating pump 2 was set to 3 mL / h, and the temperature of the insulated box 5 was set to 37°C. At the beginning of the experiment, under the action of the flow regulating pump 2, the liquid in the main tank body 4 passed through the cylindrical liquid storage bottle 11 and the rectangular liquid storage bottle 14, and some substances penetrated the biomembrane and merged into the flowing liquid, and flowed together to the liquid storage bottle 9.

[0028] The working principle and working process of the utility model are as follows: before the operation of the device of the utility model, liquid is loaded into the main tank body 4 according to the experimental needs, and a double-hole piston 3, a hose 8 and a flow regulating pump 2 are installed. The flow regulating pump 2 can adjust the flow rate according to the experimental needs; the insulated box 5 simulates the actual temperature in a specific organism; before the simulator I 6 and the simulator II 1 are installed, the cylindrical liquid storage bottle 11 and the rectangular prism-shaped liquid storage bottle 14 contain the liquid or solid required for the experiment; after the simulator I 6 and the simulator II 1 are installed, the liquid in the main tank body 4 flows through the simulator I 6 and the simulator II 1 under the control of the flow regulating pump 2, realizing the simulation of the liquid flowing through the membrane and flowing through the contact with a certain substance, and the cylindrical liquid storage bottle 11 and the rectangular prism-shaped liquid storage bottle Part of the substance in 14 is absorbed into the flowing liquid through the biomembrane and finally flows into the liquid storage bottle 9, and the liquid in the liquid storage bottle 9 is taken for inspection; in order to simulate the two different transmembrane contact situations of a certain substance in the organism, two different simulators are designed, namely simulator I6 and simulator II1, among which simulator I6 is a simulation of liquid flowing through the membrane and contacting a certain substance. It is seamlessly closed, has good sealing performance, is easy to install as a whole, and can control the flow rate uniformly; simulator II1 is a simulation of liquid flowing through the membrane and contacting a certain substance. Simulator II1 has a compact structure, and the design of the rectangular prism-shaped tube is conducive to fixing the rectangular prism-shaped liquid storage bottle 14, so that the rectangular biomembrane 16 in the middle is not easy to deform, which is conducive to the stable progress of the experiment.

Claims

1. A device for simulating transmembrane contact of substances in a living body, comprising a simulator II (1), a flow regulating pump (2), a double-hole piston (3), a main tank (4), an insulated box (5), a simulator I (6), a thermos bottle (7), a hose (8), a liquid storage bottle (9), an upper closed tube (10), a cylindrical liquid storage bottle (11), a circular biofilm (12), a lower closed tube (13), a rectangular prism-shaped liquid storage bottle (14), a rectangular prism-shaped tube (15) and a rectangular biofilm (16), characterized in that The main tank body (4) is provided with an opening at the top, and a double-hole piston (3) is installed in the opening. The two through holes of the double-hole piston (3), one of which is connected to a flow regulating pump (2), a simulator II (1) and a liquid storage bottle (9) in sequence through a hose (8), and the other through hole, a flow regulating pump (2), a simulator I (6) and a liquid storage bottle (9) in sequence through a hose (8), and the end of the hose (8) in the main tank body (4) is located below the liquid level in the main tank body (4), the simulator II (1) and the tube body connected to the simulator II (1) are located in a thermos bottle (7), the simulator I (6) and the tube body connected to the simulator I (6) are located in another thermos bottle (7), and the simulator II (1), the flow regulating pump (2), the double-hole piston (3), the main tank body (4), the simulator I (6) and the thermos bottle (7) are all located in the heat preservation box (5); The simulator I (6) comprises a lower closed tube (13), a circular biofilm (12), a cylindrical liquid storage bottle (11), a circular biofilm (12) and an upper closed tube (10) which are sequentially installed from bottom to top. The lower closed tube (13) and the upper closed tube (10) are buckled and sealed. The two circular biofilms (12) and the cylindrical liquid storage bottle (11) are located in a space formed by the buckling of the lower closed tube (13) and the upper closed tube (10). The outer sides of the lower closed tube (13) and the upper closed tube (10) are both provided with connecting tubes, which are connected to the hose (8); The simulator II (1) includes a rectangular prism-shaped liquid storage bottle (14), a rectangular biofilm (16), and a rectangular prism-shaped tube (15) installed in sequence from left to right. The side extension of the rectangular prism-shaped tube (15) and the side of the rectangular prism-shaped liquid storage bottle (14) are buckled and sealed. The rectangular biofilm (16) is located in the space formed by the buckling of the rectangular prism-shaped tube (15) and the rectangular prism-shaped liquid storage bottle (14). Connecting pipes are respectively provided at the top and bottom of the rectangular prism-shaped tube (15), and the connecting pipes are connected to the hose (8).

2. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The heat preservation box (5) is provided with a temperature control device.

3. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The thermos bottle (7) is filled with a heat preservation liquid.

4. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The upper closed tube (10) contacts the circular biofilm (12), and the contact surface of the upper closed tube (10) is mesh-shaped.

5. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The upper and lower surfaces of the cylindrical liquid storage bottle (11) are in contact with the corresponding circular biofilm (12), and the upper and lower contact surfaces of the cylindrical liquid storage bottle (11) are both mesh-shaped.

6. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The lower closed tube (13) contacts the circular biofilm (12), and the contact surface of the lower closed tube (13) is mesh-shaped.

7. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The rectangular prism-shaped liquid storage bottle (14) contacts the rectangular biofilm (16), and the contact surface of the rectangular prism-shaped liquid storage bottle (14) is in a mesh shape.

8. The device for simulating transmembrane contact of substances in a living body according to claim 1, characterized in that The rectangular prism-shaped tube (15) contacts the rectangular biofilm (16), and the contact surface of the rectangular prism-shaped tube (15) is in a mesh shape.