Embryo culture dish convenient for collecting culture solution

Abstract

The embryo culture dish comprises a dish body and a dish cover, a plurality of groups of culture grooves are formed in the inner surface of the bottom of the dish body, the culture grooves in each group are not communicated, each group of culture grooves comprises a first hole groove and a second hole groove, the first hole grooves and the second hole grooves are in one-to-one correspondence and are communicated through through grooves, and the first hole grooves are communicated with the second hole grooves through the through grooves. Each first hole groove is connected with a second hole groove through a through groove, so that the situation that culture solutions of a plurality of embryos are mixed together to cause mutual pollution is avoided, and after culture is finished, the embryos in the first hole grooves are sucked away through suction pipes for detection or transplantation; the pipettor gun head is inserted into the bottom of the second hole groove, all the culture solution is slowly sucked out for noninvasive embryo detection, and the situation that due to inconvenient operation, mineral oil covering the upper portion of the culture solution easily pollutes the whole culture solution and interferes with follow-up detection or transplantation is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of embryo and embryo manipulation and culture in human assisted reproductive technology, and more specifically to an embryo culture dish for convenient collection of culture fluid. Background Art

[0002] Currently, embryo culture dishes are indispensable tools in assisted reproductive technology. They hold embryo culture medium and mineral oil, providing a semi-enclosed environment for in vitro embryo culture. Traditional culture dishes consist of a round body and a slightly larger lid, typically made of glass or plastic. During embryo culture, a large amount of material is exchanged between cells and the culture medium. Studies have shown that the free DNA and metabolites released by embryos into the culture medium can serve as novel molecular markers for embryo quality assessment. Therefore, collecting culture medium for non-invasive embryo testing has important clinical application value.

[0003] However, the existing patent publication number: CN204455129U, which relates to the field of gamete and embryo manipulation and culture in human assisted reproductive technology, specifically a new embryo culture dish. The dish body includes a transparent flat cylindrical dish and a transparent marking and positioning layer, which is arranged on the bottom wall of the transparent flat cylindrical dish. The dish body has a diameter of 35mm or 60mm.

[0004] While this solves the problem of conventional culture dishes requiring serial number marking during use, freeing laboratory staff from arduous, delicate, and repetitive labor and improving labor efficiency, accurate and uniform position marking and positioning facilitate standardization of droplet volumes during embryo culture dish preparation, thus ensuring quality control of embryo culture dish preparation. However, during aspiration of the culture medium, the inconvenient operation can easily cause the mineral oil covering the culture medium to contaminate the entire culture medium and interfere with subsequent testing. Utility Model Content

[0005] In view of this, in order to solve the above problems, the present invention proposes an embryo culture dish for convenient collection of culture fluid, including a dish body 1 and a dish cover 2. The inner surface of the bottom of the dish body 1 is provided with multiple groups of culture grooves, and each group of culture grooves is not connected. Each group of culture grooves includes a first hole groove 3 and a second hole groove 4. The first hole groove 3 corresponds to the second hole groove 4 one by one and is connected through a through groove 5. Each first hole groove 3 is connected to a second hole groove 4 through the through groove 5 to avoid the culture fluids of multiple embryos from mixing together and causing mutual contamination. After the breeding is completed, the embryos in the first hole groove 3 are sucked out with a straw for detection or transplantation; the pipette tip 8 is inserted into the bottom of the second hole groove 4, and all the culture fluid is slowly sucked out for non-invasive embryo detection, avoiding the problem that the mineral oil covering the upper part of the culture fluid is easily contaminated due to inconvenient operation and interferes with subsequent detection or transplantation.

[0006] An embryo culture dish for convenient collection of culture fluid comprises a dish body 1 and a dish cover 2, wherein the dish cover 2 is arranged on the upper part of the dish body 1, and is characterized in that: a plurality of groups of culture grooves are provided on the inner surface of the bottom of the dish body 1, and each group of culture grooves is not connected to each other, and each group of culture grooves comprises a first hole groove 3 and a second hole groove 4, wherein the first hole groove 3 corresponds to the second hole groove 4 one-to-one and is connected through a through groove 5, and each first hole groove 3 is connected to a second hole groove 4 through the through groove 5, so as to prevent the culture fluids of multiple embryos from mixing together and causing mutual contamination.

[0007] Furthermore, the plurality of culture tanks are formed by wet etching technology.

[0008] Furthermore, the diameter of the first hole groove 3 is larger than the diameter of the second hole groove 4. Each first hole groove 3 is used to place a culture medium and an embryo for single embryo culture, and the second hole groove 4 is used for the pipette tip 8 to absorb the culture medium.

[0009] Furthermore, the first hole slot 3 and the second hole slot 4 are both arc structures or rectangular structures with the bottoms sealed and the openings facing upward.

[0010] Steps:

[0011] When using the culture dish, first add the embryo culture medium 6 into the corresponding first hole groove 3 respectively. The volume of the culture medium is usually 15-20 μL. The culture liquid will pass through the corresponding through groove 5 and enter the corresponding second hole groove 4. Then add a certain volume of paraffin oil 7 to the dish body 1 to cover the entire bottom of the dish body 1, and then cover the dish cover 2 and place the culture dish in a constant temperature incubator for embryo culture; after the culture is completed, use a straw to suck out the embryos in the first hole groove 3 for detection or transplantation; insert the pipette tip 8 into the bottom of the second hole groove 4, and slowly suck out all the culture medium for non-invasive embryo detection.

[0012] The beneficial effects of the present invention are as follows: the present invention proposes an embryo culture dish for convenient collection of culture fluid, comprising a dish body 1 and a dish cover 2; a plurality of groups of culture grooves are provided on the inner surface of the bottom of the dish body 1; the culture grooves of each group are not connected; each group of culture grooves comprises a first hole groove 3 and a second hole groove 4; the first hole groove 3 corresponds to the second hole groove 4 one-to-one and is connected through a through groove 5; each first hole groove 3 is connected to a second hole groove 4 through a through groove 5, so as to avoid the culture fluids of multiple embryos from mixing together and causing mutual contamination; after the breeding is completed, the embryos in the first hole groove 3 are sucked out with a straw for detection or transplantation; the pipette tip 8 is inserted into the bottom of the second hole groove 4, and all the culture fluid is slowly sucked out for non-invasive embryo detection, thereby avoiding the contamination of the entire culture fluid by the mineral oil covering the upper part of the culture fluid due to inconvenient operation and interference with subsequent detection or transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is an overall structural diagram of the embryo culture dish of the present invention for convenient collection of culture fluid.

[0014] Figure 2 The utility model is a cross-sectional schematic diagram of an embryo culture dish for convenient collection of culture fluid.

[0015] Figure 3 This is a schematic diagram of the usage of the embryo culture dish of the present invention for conveniently collecting culture fluid.

[0016] Description of main component symbols

[0017] Dish body 1, dish cover 2, first hole groove 3, second hole groove 4, through groove 5, embryo culture medium 6, paraffin oil 7, pipette tip 8.

[0018] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION Example

[0019] like Figure 1-2 As shown, an embryo culture dish for convenient collection of culture fluid, comprising a dish body 1 and a dish cover 2, the dish cover 2 being arranged to cover the upper portion of the dish body 1, characterized in that: a plurality of groups of culture grooves are provided on the inner surface of the bottom of the dish body 1, each group of the culture grooves being disjoint, each group of the culture grooves comprising a first hole groove 3 and a second hole groove 4, the first hole grooves 3 corresponding one-to-one with the second hole grooves 4 and being connected via a through groove 5, each first hole groove 3 being connected to a second hole groove 4 via the through groove 5, to prevent culture fluids of multiple embryos from mixing together and causing mutual contamination, the plurality of groups of culture grooves being formed using wet etching technology, the diameter of the first hole groove 3 being larger than the diameter of the second hole groove 4, each first hole groove 3 being used to place a portion of culture fluid and an embryo for single embryo culture, the second hole groove 4 being used for a pipette tip 8 to aspirate the culture fluid, and the first hole groove 3 and the second hole groove 4 both being arc structures or rectangular structures with their bottoms sealed and their openings facing upward.

[0020] like Figure 3 As shown, the operation steps are:

[0021] When using the culture dish, first add the embryo culture medium 6 into the corresponding first hole groove 3 respectively. The volume of the culture medium is usually 15-20 μL. The culture liquid will pass through the corresponding through groove 5 and enter the corresponding second hole groove 4. Then add a certain volume of paraffin oil 7 to the dish body 1 to cover the entire bottom of the dish body 1, and then cover the dish cover 2 and place the culture dish in a constant temperature incubator for embryo culture; after the culture is completed, use a straw to suck out the embryos in the first hole groove 3 for detection or transplantation; insert the pipette tip 8 into the bottom of the second hole groove 4, and slowly suck out all the culture medium for non-invasive embryo detection.

[0022] The beneficial effects of the present invention are as follows: the present invention proposes an embryo culture dish for convenient collection of culture fluid, comprising a dish body 1 and a dish cover 2; a plurality of groups of culture grooves are provided on the inner surface of the bottom of the dish body 1; the culture grooves of each group are not connected; each group of culture grooves comprises a first hole groove 3 and a second hole groove 4; the first hole groove 3 corresponds to the second hole groove 4 one-to-one and is connected through a through groove 5; each first hole groove 3 is connected to a second hole groove 4 through a through groove 5, so as to avoid the culture fluids of multiple embryos from mixing together and causing mutual contamination; after the breeding is completed, the embryos in the first hole groove 3 are sucked out with a straw for detection or transplantation; the pipette tip 8 is inserted into the bottom of the second hole groove 4, and all the culture fluid is slowly sucked out for non-invasive embryo detection, thereby avoiding the contamination of the entire culture fluid by the mineral oil covering the upper part of the culture fluid due to inconvenient operation and interference with subsequent detection or transplantation.

[0023] The above-described embodiments merely represent several implementation methods of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. An embryo culture dish for convenient collection of culture fluid, comprising a dish body (1) and a dish cover (2), wherein the dish cover (2) is arranged on the upper part of the dish body (1), and is characterized in that: A plurality of culture grooves are provided on the inner surface of the bottom of the dish body (1), and each group of culture grooves is not connected to each other. Each group of culture grooves includes a first hole groove (3) and a second hole groove (4), and the first hole groove (3) corresponds to the second hole groove (4) one by one and is connected through the through groove (5). Each first hole groove (3) is connected to a second hole groove (4) through the through groove (5), so as to avoid the culture fluids of multiple embryos from mixing together and causing mutual contamination.

2. The embryo culture dish for convenient collection of culture medium according to claim 1, characterized in that: The plurality of culture tanks are formed by adopting wet etching technology.

3. The embryo culture dish for convenient collection of culture medium according to claim 1, characterized in that: The diameter of the first hole groove (3) is larger than the diameter of the second hole groove (4). Each first hole groove (3) is used to place a portion of culture medium and embryo for single embryo culture, and the second hole groove (4) is used for a pipette tip (8) to absorb the culture medium.

4. The embryo culture dish for convenient collection of culture medium according to claim 1, characterized in that: The first hole groove (3) and the second hole groove (4) are both arc structures or rectangular structures with the bottoms sealed and the openings facing upwards.

Images