Microbial inoculation device applied to plant leaves

Abstract

The utility model discloses a microorganism inoculation device applied to plant leaves, which comprises a plurality of fitting protection pieces, round bulges are arranged in the fitting protection pieces, accommodating cavities are formed in the bottoms of the round bulges, and disinfection cotton balls and target fungus cakes are sequentially arranged in the accommodating cavities from top to bottom. And two mounting grooves are formed in the bottom of each attaching protection piece, and high-temperature-resistant adhesives are arranged in the two mounting grooves. The utility model mainly has the following advantages: 1, the requirement on the inoculation environment is not too high, and the inoculation can be carried out under the conditions of high temperature and high humidity; 2, the inoculation method realizes the effects of wind resistance, high temperature resistance and rain resistance; 3, one leaf can realize inoculation of different strains or a plurality of fungus cakes of the same strain; 4, only the positions for inoculating the fungus cakes need to be covered with plastic, so that the fungus cakes are light-permeable and breathable, and field planting and disease attack are facilitated; and 5, the inoculation method is simple, and a plurality of operation steps can be completed indoors.

Description

Technical Field

[0001] The utility model relates to the technical field of plant leaf inoculation, in particular to a microorganism inoculation device applied to plant leaves. Background Art

[0002] In current plant disease research, the isolation of pathogens and the determination of pathogenicity are very critical. Usually, diseased tissues with obvious symptoms are collected first to isolate the pathogens in the diseased tissues and purify them. Then, the fungus cake or spore liquid is inoculated into healthy plants to observe whether they become diseased. However, many strains still have difficulty producing spores even after being induced, which makes fungus cake inoculation irreplaceable.

[0003] The current inoculation of microbial cakes on living plant leaves has high environmental requirements and needs to be carried out on sunny or cloudy days with low wind, shade, and moderate humidity.

[0004] Although the existing method of inoculating microbial cakes on living plant leaves can achieve pathogenicity determination, it still has the following shortcomings:

[0005] 1. After inoculation, in order to prevent the mushroom cake from being blown off by the wind, it is necessary to put it in a fresh-keeping bag or a self-sealing bag, which is time-consuming and labor-intensive. In addition, if the wind speed is high, the newly inoculated mushroom cake will still be blown off.

[0006] 2. Usually, in order to prevent the mushroom cake from being blown off by the wind, it is necessary to use cotton to fix the mushroom cake and also to moisturize it. However, this operation is very easy to cause contamination because there are endophytes in the leaves themselves. If the target mushroom cake is slowly colonized, this will allow the endophytes to grow first.

[0007] 3. Under high temperature conditions, the culture medium in the bacterial cake will dry up, causing the inoculated strain to lose its vitality before it is successfully colonized, thereby reducing its infectivity;

[0008] 4. When watering after inoculation but before the disease occurs, special attention should be paid to the fact that water will cause the fungus cake to fall off the leaves;

[0009] 5. If the inoculated cake is completely covered, the strain will be blocked from light and air, affecting its growth and disease progression;

[0010] 6. It is difficult to inoculate different strains on one leaf.

[0011] Therefore, it is necessary to provide a microbial inoculation device for plant leaves to solve the above technical problems. Utility Model Content

[0012] The purpose of the utility model is to solve the shortcomings of the prior art and to propose a microorganism inoculation device for plant leaves.

[0013] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: a microbial inoculation device for plant leaves, comprising a plurality of fitted protective sheets, a plurality of said fitted protective sheets are provided with circular protrusions inside, a receiving cavity is provided at the bottom of said circular protrusion, a sterilized cotton ball and a target bacterial cake are provided in sequence from top to bottom inside said receiving cavity, a plurality of said fitted protective sheets are provided with two mounting grooves at the bottom, a high-temperature resistant adhesive is provided inside the two mounting grooves, the sterilized cotton ball is a sterilized wet cotton ball for achieving a moisturizing effect, the depth of said mounting groove matches the height of said high-temperature resistant adhesive to reduce the risk of the fitted protective sheet. The gap behind the fitted leaves is filled with fitted protective sheets and high-temperature resistant adhesive, so that the entire inoculation device does not have excessively high requirements for the inoculation environment. Inoculation can be carried out under high temperature and high humidity conditions, achieving the effects of wind resistance, high temperature resistance, and rain resistance. The mushroom cakes are bonded with a smaller area of ​​fitted protective sheets, replacing the traditional wrapping leaf protection. Only the position where the mushroom cakes are inoculated needs to be covered with plastic, making the mushroom cakes light-permeable and breathable, which is conducive to colonization and disease. At the same time, the design of multiple sets of tearable fitted protective sheets allows one leaf to be inoculated with different strains or multiple mushroom cakes of the same strain. At the same time, personnel can tear off the number of fitted protective sheets according to their needs, and the operation is simple and convenient.

[0014] As a further description of the above technical solution:

[0015] A protective film is attached to the bottom of the high-temperature resistant adhesive, and the protective film is used to seal the adhesive surface between the high-temperature resistant adhesive and the blade.

[0016] As a further description of the above technical solution:

[0017] The circular protrusion is arranged at the center of the fitting protection sheet, and the circular protrusion and the fitting protection sheet are made of the same material.

[0018] As a further description of the above technical solution:

[0019] The plurality of fitting protection sheets are bonded together by adhesive, and the adhesive between the plurality of fitting protection sheets is tearable adhesive.

[0020] As a further description of the above technical solution:

[0021] The inner wall of the accommodating cavity is fixedly connected with a plurality of anti-slip protrusions, and the tops of the plurality of anti-slip protrusions are provided with mounting grooves, and the mounting grooves are used to hook the sterilized cotton ball placed in the accommodating cavity.

[0022] As a further description of the above technical solution:

[0023] The laminating protective sheet is made of polyethylene, polypropylene and polyvinyl chloride.

[0024] As a further description of the above technical solution:

[0025] The culture medium side of the target bacterial cake faces the sterilized cotton ball, and the hyphae side of the target bacterial cake faces the plant leaf attachment surface.

[0026] The utility model has the following beneficial effects:

[0027] Compared with the existing technology, the microbial inoculation device applied to plant leaves has the following advantages:

[0028] 1. By setting up the protective sheet and high-temperature resistant adhesive, the entire inoculation device has no high requirements on the inoculation environment and can be used in high temperature and high humidity conditions;

[0029] 2. The protective sheet is bonded to the blade surface using a high-temperature resistant adhesive. This inoculation method achieves wind resistance, high temperature resistance, and rain resistance.

[0030] 3. One leaf can be used to inoculate different strains or multiple cakes of the same strain;

[0031] 4. The bacterial cake is bonded to a smaller protective sheet, replacing the traditional leaf-wrapped protection. Only the inoculated bacterial cake location needs to be covered with plastic, making the bacterial cake light-permeable and breathable, which is conducive to colonization and disease development.

[0032] 5. The inoculation method is simple, and many operation steps can be completed indoors first, reducing the time for inoculation in the field. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is an overall three-dimensional diagram of a microbial inoculation device for plant leaves proposed in the present invention;

[0034] Figure 2 This is a three-dimensional back view of the overall microbial inoculation device for plant leaves proposed in the present invention;

[0035] Figure 3 This is a schematic cross-sectional structure diagram of a fitted protective sheet of a microbial inoculation device for plant leaves proposed in the present invention;

[0036] Figure 4 This is a schematic diagram of the bottom structure of a fitted protective sheet of a microbial inoculation device for plant leaves proposed in the present invention;

[0037] Figure 5 for Figure 4 A partial enlarged view of point A in the middle;

[0038] Figure 6 for Figure 3 A partial enlarged view of point B in the middle.

[0039] Legend:

[0040] 1. Circular protrusion; 2. Fitting protective sheet; 3. Disinfectant cotton ball; 4. Target bacterial cake; 5. Accommodation cavity; 6. Installation slot; 7. Anti-slip bump; 8. Mounting slot; 9. High-temperature resistant adhesive; 10. Protective film. DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0042] Example 1: Reference Figure 1-4 、 Figure 6 The present invention provides a microbial inoculation device for plant leaves: it includes several fitted protective sheets 2, several of which are internally provided with circular protrusions 1, and an accommodating cavity 5 is provided at the bottom of the circular protrusion 1. A sterilized cotton ball 3 and a target bacterial cake 4 are sequentially arranged inside the accommodating cavity 5 from top to bottom, and two mounting grooves 6 are provided at the bottom of the several fitted protective sheets 2. High-temperature resistant viscose 9 is provided inside the two mounting grooves 6. The sterilized cotton ball 3 is a wet cotton ball after disinfection, which is used to achieve a moisturizing effect. The depth of the mounting groove 6 matches the height of the high-temperature resistant viscose 9 to reduce the gap after the fitted protective sheet 2 is fitted to the leaves, thereby preventing moisture from contacting the high-temperature resistant viscose 9 when it rains.

[0043] A protective film 10 is attached to the bottom of the high-temperature resistant adhesive 9, and the protective film 10 is used to seal the adhesive surface between the high-temperature resistant adhesive 9 and the blade.

[0044] The circular protrusion 1 is arranged at the center of the fitting protection sheet 2 , and the circular protrusion 1 and the fitting protection sheet 2 are made of the same material.

[0045] The plurality of bonding protection sheets 2 are bonded together by adhesive, and the adhesive between the plurality of bonding protection sheets 2 is tearable adhesive, so that a person can tear off a corresponding number of bonding protection sheets 2 as needed.

[0046] The laminating protective sheet 2 is made of polyethylene, polypropylene and polyvinyl chloride.

[0047] The culture medium side of the target bacterial cake 4 faces the sterilized cotton ball 3 , and the hyphae side of the target bacterial cake 4 faces the plant leaf contact surface.

[0048] Working principle:

[0049] The specific production and use process is as follows:

[0050] 1. First, make a fitting protective sheet 2 of the vaccination device. The device is made of high-density polyethylene, polypropylene, or polyvinyl chloride. The device has a square shape and a circular package inside.

[0051] 2. Before inoculating the cake, place the protective sheet 2 of the inoculation device on a sterile operating table and turn on the ultraviolet disinfection lamp for about 30 minutes. Then put a small ball of sterilized cotton ball 3 that has been sterilized by high temperature and high pressure into the round bag to achieve a moisturizing effect.

[0052] 3. Place the target bacterial cake 4 on top of the sterilized cotton ball 3, with the mycelia of the target bacterial cake 4 facing outward and the culture medium (without mycelia) facing the sterilized cotton ball 3;

[0053] 4. Use an insect needle to gently pierce the plant leaf, then tear off the protective film 10 of the high-temperature resistant adhesive 9 of the inoculation device, inoculate the inoculation device with the target bacterial cake 4 to the punctured area, and gently press the edge to make the high-temperature resistant adhesive 9 stick to the leaf;

[0054] 5. After 2 to 10 days of completing the above steps, you can observe whether the inoculated area and the surrounding areas of the target plant leaves are pathogenic.

[0055] Example 2: Reference Figure 4-5 This embodiment is basically the same as the previous embodiment, except that:

[0056] The inner wall of the accommodating cavity 5 is fixedly connected with several anti-slip protrusions 7, and the tops of several of the anti-slip protrusions 7 are provided with mounting grooves 8. The mounting grooves 8 are used to hook the sterilized cotton ball 3 placed in the accommodating cavity 5 to prevent it from falling off when people carry the vaccination device and move.

[0057] As can be seen from the above, when in use, the personnel insert the sterilized cotton ball 3 into the interior of the accommodating cavity 5, and then slightly twist it so that part of the sterilized cotton ball 3 is hooked with the edge of the mounting groove 8 on the anti-fall-off protrusion 7 to prevent the sterilized cotton ball 3 from falling off during movement, thereby effectively improving the safety and stability of the use of the inoculation device.

[0058] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A microbial inoculation device for plant leaves, comprising a plurality of fitted protective sheets (2), characterized in that: A circular protrusion (1) is provided inside the plurality of the fitted protective sheets (2), a receiving cavity (5) is provided at the bottom of the circular protrusion (1), a sterilized cotton ball (3) and a target bacterial cake (4) are provided inside the receiving cavity (5) in order from top to bottom, and two mounting grooves (6) are provided at the bottom of the plurality of the fitted protective sheets (2), and high-temperature resistant adhesive (9) is provided inside the two mounting grooves (6).

2. A microbial inoculation device for plant leaves according to claim 1, characterized in that: A protective film (10) is attached to the bottom of the high-temperature resistant adhesive (9).

3. The microbial inoculation device for plant leaves according to claim 1, characterized in that: The circular protrusion (1) is arranged at the center of the fitting protection sheet (2).

4. The microbial inoculation device for plant leaves according to claim 1, characterized in that: The plurality of fitting protective sheets (2) are bonded together by adhesive.

5. The microbial inoculation device for plant leaves according to claim 1, characterized in that: A plurality of anti-slip protrusions (7) are fixedly connected to the inner wall of the accommodating cavity (5), and a mounting groove (8) is formed on the top of each of the anti-slip protrusions (7).

6. The microbial inoculation device for plant leaves according to claim 1, characterized in that: The laminating protective sheet (2) is made of polyethylene, polypropylene and polyvinyl chloride.

7. The microbial inoculation device for plant leaves according to claim 1, characterized in that: The culture medium side of the target bacterial cake (4) faces the sterilized cotton ball (3), and the hyphae side of the target bacterial cake (4) faces the plant leaf contact surface.

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