Biocontrol microorganism fermentation culture equipment

Abstract

The utility model relates to the technical field of microbial fermentation culture, in particular to biocontrol microbial fermentation culture equipment which comprises a fermentation culture cabinet, the temperature controller is mounted on the rear side of the fermentation culture cabinet; the plurality of culture drawers are slidably mounted in the fermentation culture cabinet and are symmetrically distributed based on the partition rectangular frame, culture frames are movably inserted into the culture drawers, water inlet pipe heads are fixedly mounted at the ends, extending into the fermentation culture cabinet, of the culture drawers, and the front ends of the water inlet pipe heads are conical and are provided with water inlet holes; an overflow pipe is installed on one side of the culture drawer, a water return groove is formed in the portion, below the sliding groove, of the partition rectangular frame, and the water return groove communicates with a water return pipe through a guide pipe; the water inlet pipe is mounted on the fermentation culture cabinet, and a butt joint assembly communicated with the water inlet pipe head is mounted on the water inlet pipe. The biocontrol microorganism fermentation culture equipment is simple in structure, can use various culture media for unified fermentation culture, and is convenient and fast in temperature control.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial fermentation culture, in particular to a biocontrol microbial fermentation culture device. Background Art

[0002] Biocontrol using biocontrol bacteria that have antagonistic effects on tobacco Fusarium oxysporum not only effectively inhibits tobacco root rot and black shank caused by Fusarium oxysporum, but also boasts high efficiency, is pollution-free, and leaves no residue compared to chemical control methods. This makes biological control a key approach to preventing and controlling tobacco root and stem diseases in the future. Due to the numerous variants of tobacco Fusarium oxysporum and its numerous pathogenic factors, it is necessary to screen for highly effective antagonistic strains from healthy tobacco plants in diseased areas, isolate and extract their key metabolites, and ultimately optimize fermentation conditions to increase metabolite production and develop highly effective biocontrol microbial agents. Therefore, in relevant research, it is necessary to use a variety of different matrices to study their effects on the corresponding metabolites and production of microorganisms. However, existing microbial fermentation and cultivation equipment mostly consists of a single fermentation tank, which not only has poor internal temperature control, which can easily lead to the death of microorganisms in the core due to heat, but also cannot be replaced with different matrices within the same culture cycle. Utility Model Content

[0003] In order to solve the above technical problems, the utility model provides a biocontrol microorganism fermentation and culture equipment that uses multiple culture media for unified fermentation and culture, and has convenient temperature control.

[0004] The biocontrol microorganism fermentation and cultivation equipment provided by the utility model comprises: a fermentation and cultivation cabinet, and a partition rectangular frame arranged in the middle of the fermentation and cultivation cabinet, and a cabinet door is provided on the front side of the fermentation and cultivation cabinet;

[0005] A temperature controller is installed on the rear side of the fermentation culture cabinet;

[0006] A culture drawer, wherein several culture drawers are slidably installed in the fermentation culture cabinet and are symmetrically distributed based on the partition rectangular frame, and a culture frame is movably inserted into the culture drawer, and a water inlet pipe head is fixedly installed at one end of the culture drawer extending into the fermentation culture cabinet, and the front end of the water inlet pipe head is tapered and has a water inlet hole. An overflow pipe is installed on one side of the culture drawer, and a slide groove for sliding the overflow pipe is opened on the partition rectangular frame. A return water trough is installed on the partition rectangular frame below the slide groove, and the return water trough is connected to the return water pipe through a conduit;

[0007] A water inlet pipe is installed on the fermentation culture cabinet, and a docking assembly connected to the water inlet pipe head is installed on the water inlet pipe;

[0008] a heat exchange water tank, which is arranged at the bottom end of the fermentation and cultivation cabinet, and is exposed to the outside world and realizes heat exchange with the outside world; the heat exchange water tank is filled with heat exchange water; the bottom end of the return water pipe is connected to the heat exchange water tank through a conduit and is arranged at the bottom end of the fermentation and cultivation cabinet; an electric heating rod and a temperature sensor are provided in the heat exchange water tank; the input end of the electric heating rod is electrically connected to the output end of the thermostat, and the output end of the temperature sensor is electrically connected to the output end of the thermostat;

[0009] A circulation pump is arranged at the top of the fermentation culture cabinet, and the water inlet end of the circulation pump is connected to the heat exchange water tank, and the water outlet end of the circulation pump is connected to the water inlet pipe.

[0010] Preferably, the culture drawer is embedded with transparent glass and a handle at one end facing the outside of the fermentation culture cabinet, a number of punching frames are evenly installed in the culture drawer, a sponge strip is embedded in the punching frame, a number of slots are evenly opened in the culture frame, the slots are plugged into the punching frame, and support rods are fixedly installed at the four corners of the bottom end of the culture frame.

[0011] Preferably, positioning pins are symmetrically provided on both sides of the top of the culture drawer, and positioning plates are installed on both sides of the culture frame. Positioning holes are provided on the positioning plates to be plugged into and matched with the positioning pins.

[0012] Preferably, a pull rod is fixedly mounted on the positioning plate.

[0013] Preferably, the height of the water inlet pipe head is lower than that of the overflow pipe, and the height of the overflow pipe is lower than the bottom surface of the culture frame.

[0014] Preferably, the docking assembly includes a plug-in tube, which is fixedly mounted on the water inlet pipe, and a transfer tube is installed on the plug-in tube, the transfer tube is plugged into and matched with the water inlet pipe head, and a valve seat is installed in the transfer tube, a guide rod is movably inserted on the end plate of the plug-in tube, and a conical valve core is fixedly sleeved on one end of the guide rod extending into the transfer tube, a limit spring is sleeved on the guide rod, the limit spring is fixedly connected to the end plate of the plug-in tube, and a through hole is opened on the end plate of the plug-in tube.

[0015] Compared with related technologies, the biocontrol microbial fermentation and cultivation equipment provided by the present invention has the following beneficial effects:

[0016] The utility model provides a sliding culture drawer in the fermentation culture cabinet, and a movable culture frame is provided on the culture drawer. The culture frame can be movably taken out and the culture frames in different culture drawers can contain different culture media, which is convenient for later confirmation of the most suitable culture medium type.

[0017] By arranging the water inlet pipe head on the culture drawer and cooperating with the plug-in pipe, transfer pipe, valve seat, guide rod, conical valve core, limit spring and through hole of the docking assembly, the water supply can be disconnected when it is pulled out and water can be automatically fed in when it is pushed back. By utilizing the cooperation of the overflow pipe and the return pipe, the hot water can be circulated inside each drawer, and the heat inside the drawer can be exchanged in time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A schematic structural diagram of a preferred embodiment of the biocontrol microbial fermentation and cultivation equipment provided by the present invention;

[0019] Figure 2 This is a structural schematic diagram of the biocontrol microorganism fermentation and cultivation equipment provided by the utility model from another perspective;

[0020] Figure 3 This is a schematic diagram of the structure of the culture drawer and culture frame provided by the utility model;

[0021] Figure 4 This is a structural schematic diagram of the culture drawer and culture frame provided by the utility model from another perspective;

[0022] Figure 5 A schematic diagram of the structure of a water return pipe connected to a water return tank provided by the present invention;

[0023] Figure 6 This is a structural diagram of the water inlet pipe provided by the utility model being connected to a docking assembly;

[0024] Figure 7 This is a structural diagram of the connection between the docking assembly and the water inlet pipe head. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0026] The specific implementation of the present invention is described in detail below with reference to specific embodiments.

[0027] See also Figures 1 to 7 The present invention provides a biocontrol microorganism fermentation and cultivation device, which includes:

[0028] A fermentation and culture cabinet 1, and a partition rectangular frame 11 provided in the middle of the fermentation and culture cabinet 1, and a cabinet door 12 is provided on the front side of the fermentation and culture cabinet 1;

[0029] The temperature controller 2 is installed on the rear side of the fermentation culture cabinet 1;

[0030] There are several culture drawers 3, and the culture drawers 3 are slidably installed in the fermentation culture cabinet 1 and symmetrically distributed based on the partition rectangular frame 11. The culture frame 4 is movably inserted into the culture drawer 3. The end of the culture drawer 3 extending into the fermentation culture cabinet 1 is fixedly installed with a water inlet pipe head 31. The front end of the water inlet pipe head 31 is tapered and has a water inlet hole 301. An overflow pipe 32 is installed on one side of the culture drawer 3. A chute 101 for sliding the overflow pipe 32 is opened on the partition rectangular frame 11. A return water trough 61 is installed on the partition rectangular frame 11 below the chute 101. The return water trough 61 is connected to the return water pipe 6 through a conduit;

[0031] The water inlet pipe 5 is installed on the fermentation culture cabinet 1, and a docking assembly 9 connected to the water inlet pipe head 31 is installed on the water inlet pipe 5;

[0032] The heat exchange water tank 7 is arranged at the bottom end of the fermentation culture cabinet 1, and the heat exchange water tank 7 is exposed to the outside world and realizes heat exchange with the outside world. At the same time, the heat exchange water tank 7 is filled with heat exchange water, and the bottom end of the return water pipe 6 is connected to the heat exchange water tank 7 through a conduit. An electric heating rod and a temperature sensor are provided in the heat exchange water tank 7. The input end of the electric heating rod is electrically connected to the output end of the thermostat 2, and the output end of the temperature sensor is electrically connected to the output end of the thermostat 2; when the temperature of the heat exchange water is too low, the thermostat 2 can control the electric heating rod to heat the heat exchange water.

[0033] The circulation pump 8 is arranged at the top of the fermentation culture cabinet 1 , and the water inlet end of the circulation pump 8 is connected to the heat exchange water tank 7 , and the water outlet end of the circulation pump 8 is connected to the water inlet pipe 5 .

[0034] The height of the water inlet pipe head 31 is lower than that of the overflow pipe 32 , and the height of the overflow pipe is lower than the bottom surface of the culture frame.

[0035] It should be noted that when in use, the culture drawer 3 is pulled out of the fermentation culture cabinet 1, the culture medium is placed on the culture frame 4, which is then movably inserted into the culture drawer 3 and pushed back into the fermentation culture cabinet 1. When pushed back, the water inlet pipe head 31 is connected to the docking component 9 to achieve conduction with the water inlet pipe 5, so that when the circulation pump 8 is started, the hot water in the hot water exchange tank 7 is continuously transported to the water inlet pipe 5, and then the hot water exchange is flowed from the water inlet hole 301 of the water inlet pipe head 31 into the culture drawer 3 through the docking component 9, and then flows into the return water tank 61 through the overflow pipe 32, and finally flows into the hot water exchange tank 7 again through the return water pipe 6 (the hot water exchange does not contact the culture frame and the culture medium in the culture frame during the whole process), thereby realizing the circulation supply of hot water exchange, facilitating the effective control of the temperature in the culture frame, and facilitating the fermentation of microorganisms. After a cultivation cycle is completed, the culture drawer 3 is pulled out, and then the culture frame 4 is taken out of the culture drawer 3, and new culture medium is replaced to continue cultivation. When pulled out, the water inlet pipe head 31 is disconnected from the docking component 9 to achieve water cut-off. After replacing the culture medium, the culture drawer 3 is pushed back into the fermentation culture cabinet 1. When pushed back, the water inlet pipe head 31 is re-plugged with the docking component 9 to achieve circulating water supply.

[0036] In the embodiments of the present invention, please refer to Figure 1 、 Figure 3 and Figure 4 The end of the culture drawer 3 facing the outside of the fermentation culture cabinet 1 is embedded with transparent glass 33 and a handle 34. A number of punching frames 35 are evenly installed in the culture drawer 3, and a sponge strip 36 is embedded in the punching frame 35. The culture frame 4 is evenly opened with a number of slots 401, which are plugged into the punching frame 35, and support rods 41 are fixedly installed at the four corners of the bottom end of the culture frame 4;

[0037] Positioning pins 37 are symmetrically arranged on both sides of the top of the culture drawer 3, and positioning plates 42 are installed on both sides of the culture frame 4. Positioning holes 402 are opened on the positioning plates 42 to plug and cooperate with the positioning pins 37;

[0038] A pull rod 43 is fixedly mounted on the positioning plate 42 .

[0039] It should be noted that: the culture drawer 3 is provided with transparent glass 33 to facilitate real-time observation of the internal fermentation situation. The culture frame 4 is plugged into the positioning pin 37 of the culture drawer 3 through the positioning hole 402 of the positioning plate 42, so that when the culture frame 4 is inserted into the culture drawer 3, the slot 401 is plugged into the punched frame 35. After plugging, when water circulates in the culture drawer 3, the sponge strip 36 can slow down the water flow rate, thereby achieving efficient heat exchange. After the culture is completed, the culture frame 4 can be removed by pulling the pull rod 43.

[0040] In the embodiments of the present invention, please refer to Figure 1 、 Figure 2、 Figure 4 、 Figure 5 、 Figure 6 and Figure 7 The docking assembly 9 includes a plug-in tube 91, which is fixedly mounted on the water inlet pipe 5, and a transfer tube 92 is installed on the plug-in tube 91. The transfer tube 92 is plugged into the water inlet pipe head 31, and a valve seat 93 is installed in the transfer tube 92. A guide rod 94 is movably inserted into the end plate of the plug-in tube 91, and one end of the guide rod 94 extending into the transfer tube 92 is fixedly sleeved with a conical valve core 95. A limit spring 96 is sleeved on the guide rod 94, and the limit spring 96 is fixedly connected to the end plate of the plug-in tube 91, and a through hole 901 is opened on the end plate of the plug-in tube 91.

[0041] It should be noted that: when the docking assembly 9 is docked with the water inlet pipe head 31, the water inlet pipe head 31 is inserted into the transfer pipe 92. When the water inlet pipe head 31 is inserted, the guide rod 94 is pushed backward until the conical valve core 95 is pushed away from the valve seat 93, so that the hot water in the water inlet pipe 5 flows into the culture drawer 3 through the through hole 901, the transfer pipe 92 and the water inlet hole 301 until it is higher than the overflow pipe 32. The water flows into the return water tank 61 through the overflow pipe 32, and then flows into the return water pipe 6 through the conduit, and finally flows into the hot water exchange tank 7 to realize circulating water supply. When the culture drawer 3 is pulled out of the fermentation culture cabinet 1, the water inlet pipe head 31 withdraws from the transfer pipe 92. Under the action of the limit spring 96, the guide rod 94 is squeezed to drive the conical valve core 95 to seal the valve seat 93, thereby cutting off the water supply.

[0042] The working principle of the biocontrol microorganism fermentation and cultivation equipment provided by the utility model is as follows:

[0043] During use, the culture drawer 3 is pulled out from the fermentation culture cabinet 1, the culture medium is placed on the culture frame 4, which is then movably inserted into the culture drawer 3, and then the culture drawer 3 is pushed back into the fermentation culture cabinet 1. When pushed back, the water inlet pipe head 31 is docked with the docking component 9 and connected to the water inlet pipe 5, so that when the circulation pump 8 is started, the hot water in the heat exchange tank 7 is continuously transported to the water inlet pipe 5, and then the hot water flows from the water inlet hole 301 of the water inlet pipe head 31 into the culture drawer 3 through the docking component 9, and then flows into the return water tank 61 through the overflow pipe 32, and then flows back into the heat exchange tank 7 through the return water pipe 6, thereby realizing the circulation supply of hot water, which is convenient for effectively controlling the temperature in the culture frame and is beneficial to microbial fermentation. After a cultivation cycle is completed, the culture drawer 3 is pulled out, and then the culture frame 4 is taken out of the culture drawer 3, and new culture medium is replaced to continue cultivation. When the culture drawer is pulled out, the water inlet pipe head 31 is disconnected from the docking assembly 9 to cut off the water supply. After the culture medium is replaced, the culture drawer 3 is pushed back into the fermentation culture cabinet 1 for a new round of culture. When pushing it back, the water inlet pipe head 31 is reconnected with the docking assembly 9 to achieve circulating water supply.

[0044] When the specific docking assembly 9 is docked with the water inlet pipe head 31, the water inlet pipe head 31 is inserted into the transfer pipe 92. When the water inlet pipe head 31 is inserted, the guide rod 94 is pushed backward until the conical valve core 95 is pushed away from the valve seat 93, so that the hot water in the water inlet pipe 5 flows into the culture drawer 3 through the through hole 901, the transfer pipe 92 and the water inlet hole 301 until it is higher than the overflow pipe 32. The water flows into the return water tank 61 through the overflow pipe 32, and then flows into the return water pipe 6 through the conduit, and finally flows into the hot water exchange tank 7 to realize circulating water supply. When the culture drawer 3 is pulled out of the fermentation culture cabinet 1, the water inlet pipe head 31 withdraws from the transfer pipe 92. Under the action of the limit spring 96, the guide rod 94 is squeezed to drive the conical valve core 95 to seal the valve seat 93, thereby cutting off the water supply.

[0045] The circuits and controls involved in the present invention are all prior art and will not be described in detail here.

[0046] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A biocontrol microorganism fermentation and cultivation device, comprising a fermentation and cultivation cabinet (1) and a partition rectangular frame (11) arranged in the middle of the fermentation and cultivation cabinet (1), wherein the front side of the fermentation and cultivation cabinet (1) is provided with a cabinet door (12) that opens in opposite directions, and the rear side of the fermentation and cultivation cabinet (1) is provided with a temperature controller (2), characterized in that: Also includes: A culture drawer (3), wherein a plurality of the culture drawers (3) are slidably installed in a fermentation culture cabinet (1) and symmetrically distributed based on a partition rectangular frame (11), and a culture frame (4) is movably inserted into the culture drawer (3), and a water inlet pipe head (31) is fixedly installed at one end of the culture drawer (3) extending into the fermentation culture cabinet (1), and the front end of the water inlet pipe head (31) is tapered and has a water inlet hole (301), an overflow pipe (32) is installed on one side of the culture drawer (3), a chute (101) for sliding the overflow pipe (32) is opened on the partition rectangular frame (11), and a return water trough (61) is installed on the partition rectangular frame (11) below the chute (101), and the return water trough (61) is connected to the return water pipe (6) through a conduit; A water inlet pipe (5) is installed on the fermentation culture cabinet (1), and a docking assembly (9) connected to the water inlet pipe head (31) is installed on the water inlet pipe (5); A heat exchange water tank (7) is provided at the bottom end of the fermentation culture cabinet (1), and the heat exchange water tank (7) is filled with heat exchange water. The bottom end of the return water pipe (6) is connected to the heat exchange water tank (7) through a conduit; A circulation pump (8) is provided at the top of the fermentation culture cabinet (1), and the water inlet of the circulation pump (8) is connected to the heat exchange water tank (7), and the water outlet of the circulation pump (8) is connected to the water inlet pipe (5).

2. The biocontrol microorganism fermentation and cultivation equipment according to claim 1, characterized in that: The culture drawer (3) is embedded with a transparent glass (33) and a handle (34) at one end facing the outside of the fermentation culture cabinet (1). A plurality of punching frames (35) are evenly installed in the culture drawer (3), and a sponge strip (36) is embedded in the punching frames (35). The culture frame (4) is evenly opened with a plurality of slots (401), and the slots (401) are plugged into the punching frames (35). Support rods (41) are fixedly installed at the four corners of the bottom end of the culture frame (4).

3. The biocontrol microorganism fermentation and cultivation equipment according to claim 2, characterized in that: Positioning pins (37) are symmetrically arranged on both sides of the top of the culture drawer (3), and positioning plates (42) are installed on both sides of the culture frame (4). Positioning holes (402) are provided on the positioning plates (42) to be plugged into and matched with the positioning pins (37).

4. The biocontrol microorganism fermentation and cultivation equipment according to claim 3, characterized in that: A pull rod (43) is fixedly mounted on the positioning plate (42).

5. The biocontrol microorganism fermentation and cultivation equipment according to claim 1, characterized in that: The height of the water inlet pipe head (31) is lower than that of the overflow pipe (32), and the height of the overflow pipe (32) is lower than the bottom surface of the culture frame (4).

6. The biocontrol microorganism fermentation and cultivation equipment according to claim 1, characterized in that: The docking assembly (9) comprises a plug-in tube (91), the plug-in tube (91) being fixedly mounted on the water inlet pipe (5), and a transfer tube (92) being mounted on the plug-in tube (91), the transfer tube (92) being plugged into and matched with the water inlet pipe head (31), and a valve seat (93) being mounted in the transfer tube (92), a guide rod (94) being movably inserted on the end plate of the plug-in tube (91), one end of the guide rod (94) extending into the transfer tube (92) being fixedly sleeved with a conical valve core (95), a limit spring (96) being sleeved on the guide rod (94), the limit spring (96) being fixedly connected to the end plate of the plug-in tube (91), and a through hole (901) being opened on the end plate of the plug-in tube (91).

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