Culture bottle with physical isolation

Abstract

The utility model provides a culture bottle with physical isolation, which comprises a culture bottle body and an embedded structure, the culture bottle body is used for containing nutrient solution, the culture bottle body is provided with a bottle opening, and the bottle opening is provided with a bottle opening plug for sealing the culture bottle body; the embedded structure is positioned in the culture bottle body and is provided with a containing part for containing adsorbates and an anti-leakage part for preventing the adsorbates from leaking. Compared with the prior art, the internal structure of the culture bottle is optimized, so that the nutrient solution and the resin in the culture bottle are physically isolated, and the problem that the bacterium culture capacity of the culture bottle is reduced due to contact of the nutrient solution and the resin is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of culture bottles, in particular to a culture bottle with physical isolation. Background Art

[0002] Bloodstream infection (BSI) is a general term for sepsis and bacteremia. Septicemia is caused by the invasion of the bloodstream by various pathogenic microorganisms (bacteria or fungi) and toxins. Its primary clinical manifestations include a series of severe symptoms, including sudden chills, high fever, tachycardia, rapid breathing, rash, hepatosplenomegaly, and altered mental status. Severe cases can lead to shock, disseminated intravascular coagulation (DIC), and multiple organ failure. Bacteremia is defined as short-lived bacterial invasion of the bloodstream without clinically apparent toxic symptoms (e.g., vascular-related infection). In recent years, with the widespread development of invasive diagnostic and treatment techniques and the widespread use of broad-spectrum antibiotics and hormones, the incidence of BSI has been increasing annually. BSIs carry a high mortality rate, prolong hospitalizations, and increase hospital costs, resulting in significant complications. Consequently, the control of BSIs is receiving increasing attention.

[0003] Currently, blood culture is the gold standard for diagnosing bloodstream infections. It involves inoculating a freshly isolated blood sample onto a nutrient medium under controlled conditions of temperature and humidity to allow the growth and reproduction of highly nutrient-demanding bacteria, allowing them to be identified and subsequently confirmed as pathogens. It is commonly used for the etiological diagnosis of bacteremia, fungemia, sepsis, and septicemia.

[0004] The culture medium is usually placed in a culture bottle, and an automated blood culture instrument is used to enrich the culture bottle until it tests positive, which indicates a bloodstream infection.

[0005] However, there are many factors that affect blood culture, including the composition, temperature, humidity, etc. of the culture medium. In order to ensure the accuracy of blood culture results, it is necessary to ensure that the blood culture environment is suitable and stable. Utility Model Content

[0006] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a culture bottle with physical isolation to ensure a suitable and stable environment for blood culture, specifically, to provide an environment with stable culture medium and no antibiotics.

[0007] To achieve the above-mentioned purpose and other related purposes, the technical solution of this utility model is as follows:

[0008] Culture flasks with physical isolation, including:

[0009] A culture bottle body, the culture bottle body is used to contain a nutrient solution, the culture bottle body has a bottle mouth, and the bottle mouth is provided with a bottle mouth plug for sealing the culture bottle body;

[0010] The embedded structure is located inside the culture bottle body and has a containing portion for containing the adsorbent and a leak-proof portion for preventing the adsorbent from leaking.

[0011] Optionally, the anti-leakage portion is located at the upper end of the containing portion, and the minimum inner diameter of the anti-leakage portion is smaller than the minimum inner diameter of the containing portion.

[0012] Optionally, the embedded structure further includes a connecting portion located between the anti-leakage portion and the bottle mouth, and the connecting portion allows the embedded structure to be suspended in the culture bottle body.

[0013] Optionally, the upper end of the connecting portion is engaged between the bottle mouth and the bottle mouth plug.

[0014] Optionally, the connecting portion is cylindrical, and is provided with a connecting hole that passes through the inside and outside of the embedded structure.

[0015] Optionally, there are multiple communicating holes, and the multiple communicating holes are distributed around the connecting portion.

[0016] Optionally, the communication holes are arranged in a row around the connecting portion, and the diameter of the communication holes is larger than the inner diameter of the anti-leakage portion.

[0017] Optionally, the communication holes are arranged in multiple rows around the connecting portion, and the diameter of the communication holes is smaller than the inner diameter of the anti-leakage portion.

[0018] Optionally, an embedded bottle stopper is provided in the embedded structure, and the embedded bottle stopper seals the anti-leakage part.

[0019] Optionally, the height from the lower end of the bottle mouth plug to the upper end of the communicating hole is greater than the height of the embedded bottle plug, or the inner diameter of the connecting portion at the communicating hole is greater than the outer diameter of the embedded bottle plug.

[0020] The technical solution of this patent application takes into account that in the blood culture process, the vast majority of culture targets are patients' blood, and patients are likely to have used antibiotics, and the presence of antibiotics will affect the effect of blood culture. Therefore, in addition to adding culture medium (nutrient solution) to the culture bottle, it is also necessary to add an adsorbent (resin) for adsorbing antibiotics; this patent application also takes into account that in actual use scenarios, culture medium is usually loaded into culture bottles during production, which can be convenient for sale or use. If it is necessary to add an adsorbent, it must be chosen to load the culture medium and adsorbent into the culture bottle together.

[0021] Generally speaking, there's nothing wrong with the above solution. However, research on this technical solution has revealed that after the culture bottles are manufactured (and before use), the resin used to adsorb antibiotics and the nutrient solution inside the bottles remain mixed and in contact, which can adversely affect the subsequent use of the bottles. For example, the resin absorbs a certain amount of nutrients from the nutrient solution, reducing the nutrient content and subsequently reducing the bottles' ability to cultivate bacteria. Furthermore, after the resin absorbs nutrients, its ability to adsorb antibiotics decreases. If the patient's body fluids contain antibiotics, the bottles' ability to cultivate bacteria will be significantly reduced during use.

[0022] In the present invention, the nutrient solution is contained in the culture bottle body and the resin is contained in the container portion placed inside the culture bottle, thereby achieving physical isolation between the resin and the nutrient solution. Before the culture bottle is used, during transportation and storage, if the culture bottle is subject to overturning and vibration shock, the anti-leakage portion can also try to prevent the resin from leaking from the container portion during this process. This ensures that the resin and the nutrient solution are not in contact before the culture bottle is used, avoiding the problem of reduced nutritional components in the nutrient solution caused by contact between the resin and the nutrient solution, as well as reduced ability of the resin to adsorb antibiotics, which leads to reduced bacterial capacity of the culture bottle. When the culture bottle is in use, blood can be injected into the culture bottle body through the bottle stopper, and the resin and nutrient solution can be fully mixed by inversion, shaking, etc., so that the nutrient solution provides nutrition for the culture process and the resin adsorbs antibiotics. Compared with the existing technology, the present solution achieves physical isolation between the nutrient solution and the resin in the culture bottle by optimizing the internal structure of the culture bottle, eliminating the problem of reduced bacterial capacity of the culture bottle caused by contact between the nutrient solution and the resin. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a structural diagram of a culture bottle with physical isolation according to an example of the present invention;

[0024] Figure 2 This is a structural diagram of a culture bottle with physical isolation (with an embedded bottle stopper) according to an example of the present invention;

[0025] Figure 3 This is a structural schematic diagram of a culture bottle with physical isolation according to another example of the present invention;

[0026] Figure 4 This is a structural schematic diagram of another example of a culture bottle with physical isolation (with an embedded bottle stopper) of the present invention.

[0027] The description of the reference numerals in the embodiments includes:

[0028] The culture bottle body 10 , the bottle mouth 11 , the bottle mouth plug 12 , the embedded structure 20 , the anti-leakage part 21 , the connecting part 22 , the communicating hole 23 , the embedded bottle plug 24 , and the containing part 25 . DETAILED DESCRIPTION

[0029] In the following description, numerous specific details are provided to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features known in the art are not described to avoid confusion with the present invention.

[0030] It should be understood that the present invention can be implemented in various forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided to make the disclosure thorough and complete and to fully convey the scope of the present invention to those skilled in the art. In the accompanying drawings, like reference numerals represent like components throughout.

[0031] The specific structure of the culture bottle with physical isolation described in this embodiment is combined with reference to Figures 1 to 4 , the culture bottle with physical isolation comprises:

[0032] A culture bottle body 10, wherein the culture bottle body 10 is used to contain a nutrient solution. The culture bottle body 10 has a bottle mouth 11, and a bottle mouth plug 12 is provided at the bottle mouth 11 to seal the culture bottle body 10;

[0033] The embedded structure 20 is located inside the culture bottle body 10 and has a containing portion 25 for containing the adsorbent and a leak-proof portion 21 for preventing the adsorbent from leaking.

[0034] The embedded structure 20 can be made of a hard material, such as the same material as the culture bottle body 10; the embedded structure 20 can also be made of a soft material, such as a film, silica gel or other soft materials. In this embodiment, the adsorbent is a resin that adsorbs antibiotics, and the containing portion 25 is used to contain the resin alone. The anti-leakage portion 21 can completely seal the containing portion 25 or not. For example, if the embedded structure 20 is made entirely of a soft material, the anti-leakage portion 21 can seal the containing portion 25 to prevent the resin from flowing out of the containing portion 25 and coming into contact with the nutrient solution before the culture bottle is used. When the culture bottle is used, the resin can be punctured to allow the resin to flow out and mix with the nutrient solution. If the embedded structure 20 is made of a hard material, or a combination of a hard material and a soft material, the embedded structure 20 is fixed relative to the culture bottle body 10. In this case, the anti-leakage portion 21 can be an open structure and be arranged above the containing portion 25. During the transportation and storage of the culture bottle before use, the resin can be prevented from overflowing from the containing portion 25. When the culture bottle is used, the resin can be allowed to flow out and mix with the nutrient solution by inverting the culture bottle.

[0035] It should be noted that in this solution, the nutrient solution in the culture bottle body 10 and the resin in the containing part 25 are physically isolated before the culture bottle is used, but they do not need to be completely isolated. A small amount of contact between the nutrient solution and the resin can also maintain the culture bottle's ability to culture bacteria; therefore, the leakage prevention part 21 does not need to completely seal the containing part 25. The leakage prevention part 21 only needs to prevent the resin in the containing part 25 from leaking into the nutrient solution in the culture bottle body 10 in large quantities before the culture bottle is used.

[0036] During use, the nutrient solution is contained within the culture bottle body 10, and the resin is contained within the container portion 25 placed within the culture bottle body 10, thereby achieving physical isolation between the resin and the nutrient solution. During transportation and storage before use, if the culture bottle is subject to tipping or vibration, the leak-proof portion 21 can also minimize leakage of the resin from the container portion 25. This ensures that the resin and the nutrient solution do not come into contact or have minimal contact before use, thereby avoiding the problem of reduced nutritional content in the nutrient solution due to contact between the resin and the nutrient solution, as well as a reduced ability of the resin to adsorb antibiotics, which in turn reduces the bacterial capacity of the culture bottle. During use, blood can be injected into the culture bottle body 10 through the bottle stopper 12. The resin and nutrient solution are then fully mixed by inverting and shaking the culture bottle, allowing the nutrient solution to provide nutrients for the culture process and the resin to adsorb antibiotics.

[0037] In some embodiments, the anti-leakage portion 21 is located at the upper end of the containing portion 25, and the minimum inner diameter of the anti-leakage portion 21 is smaller than the minimum inner diameter of the containing portion 25. For example, Figures 1 to 4In the embodiment, the anti-leakage portion 21 is arranged at the upper end of the containing portion 25. The inner diameter of the containing portion 25 from top to bottom can gradually increase, gradually decrease, change regularly, change irregularly, remain unchanged, etc. When the minimum inner diameter of the anti-leakage portion 21 is smaller than the minimum inner diameter of the containing portion 25 (which can be at any position of the containing portion 25), the resin contained in the containing portion 25 can be prevented from spilling out of the anti-leakage portion 21 to a great extent; during the transportation and storage of the culture bottle before use, the culture bottle is generally in a horizontal position. Although the resin in the containing portion 25 will vibrate with the culture bottle, due to the inner diameter limitation of the anti-leakage portion 21, the resin will not leak into the nutrient solution, thereby avoiding contact between the resin and the nutrient solution before the culture bottle is used.

[0038] In some embodiments, the embedded structure 20 further includes a connecting portion 22 located between the anti-leakage portion 21 and the bottle mouth 11, and the connecting portion 22 allows the embedded structure 20 to be suspended in the culture bottle body 10. For example, Figures 1 to 4 In the figure, the embedded structure 20 includes a connecting part 22, an anti-leakage part 21, and a containing part 25 from top to bottom, and the connecting part 22, the anti-leakage part 21, and the containing part 25 are an integrated structure. The setting of the connecting part 22 can make the embedded structure 20 suspended in the culture bottle, which is convenient for achieving physical isolation of the resin and the nutrient solution before the culture bottle is used and sufficient mixing of the resin and the nutrient solution when the culture bottle is used.

[0039] In some embodiments, the upper end of the connecting portion 22 is engaged between the bottle mouth 11 and the bottle mouth plug 12. For example, Figures 1 to 4 It is convenient for the production and assembly of the culture bottles in this scheme.

[0040] In some embodiments, the connecting portion 22 is cylindrical, and is provided with a connecting hole 23 that passes through the inside and outside of the embedded structure 20. For example, Figures 1 to 4 In the embodiment, the cylindrical connecting portion 22 facilitates the arrangement of the embedded structure 20 in this solution and the loading of the resin into the embedded structure 20, and the communicating hole 23 facilitates the full mixing of the resin and the nutrient solution when the culture bottle is used.

[0041] In some embodiments, there are a plurality of communicating holes 23, and the plurality of communicating holes 23 are distributed around the connecting portion 22. For example, Figures 1 to 4 It is convenient for the resin and nutrient solution to be fully mixed when the culture bottle is used.

[0042] In some embodiments, the communication holes 23 are arranged in a row around the connecting portion 22, and the diameter of the communication holes 23 is larger than the inner diameter of the anti-leakage portion 21. For example, Figure 1 and Figure 2 When the diameter of the communicating holes 23 is large, it is convenient for the resin and the nutrient solution to quickly contact and mix when the culture bottle is used, so setting one row can meet the needs.

[0043] In some embodiments, the communication holes 23 are arranged in multiple rows around the connecting portion 22, and the diameter of the communication holes 23 is smaller than the inner diameter of the anti-leakage portion 21. For example, Figure 3 and Figure 4 When the diameter of the communicating holes 23 is small, the speed at which the resin flows out of the embedded structure 20 is reduced. In order to allow the resin and the nutrient solution to contact and mix quickly, multiple rows of communicating holes 23 are provided.

[0044] In some embodiments, the embedded structure 20 is provided with an embedded bottle stopper 24, and the embedded bottle stopper 24 is used to seal the anti-leakage portion 21. For example, Figure 2 and Figure 4 In the embodiment, the embedded bottle stopper 24 can completely seal the containing part 25 at the anti-leakage part 21, ensuring that the resin and the nutrient solution are completely physically isolated before the culture bottle is used, so as to ensure the ability of the culture bottle to culture bacteria; when the culture bottle in this scheme is used, the blood to be cultured can be injected into the containing part 25 from the bottle mouth stopper 12, the connecting part 22, and the embedded bottle stopper 24 in sequence through a syringe, and the resin and blood in the containing part 25 are mixed, and the overall liquid capacity is increased. The liquid has a buoyancy effect on the embedded bottle stopper 24, which can make the embedded bottle stopper 24 float up to open the anti-leakage part 21, and there is no obstruction at the flow hole, so that the resin and blood in the containing part 25 can flow out from the flow hole and enter the culture bottle body 10 to mix, thereby realizing the culture of the blood.

[0045] In some embodiments, the height from the lower end of the bottle stopper 12 to the upper end of the communication hole is greater than the height of the embedded bottle stopper 24, or the inner diameter of the connecting portion 22 at the communication hole is greater than the outer diameter of the embedded bottle stopper 24. Figure 2 and Figure 4 In the embodiment, the anti-leakage portion 21 and the communicating hole 23 need to be separated before the embedded bottle stopper 24 floats up. When in use, the embedded bottle stopper 24 will float up due to the buoyancy of the liquid in the containing portion 25, thereby opening the communicating hole 23. In order for the communicating hole 23 to be opened smoothly, the embedded bottle stopper 24 cannot block the communicating hole 23 after floating up. Therefore, after the embedded bottle stopper 24 floats up, it either floats to the upper end of the communicating hole 23 or there is a gap between it and the communicating hole 23 after floating up.

[0046] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed in the present invention are intended to be covered by the claims of the present invention.

Claims

1. A culture bottle with physical isolation, characterized in that include: A culture bottle body, the culture bottle body is used to contain a nutrient solution, the culture bottle body has a bottle mouth, and the bottle mouth is provided with a bottle mouth plug for sealing the culture bottle body; The embedded structure is located inside the culture bottle body and has a containing portion for containing the adsorbent and a leak-proof portion for preventing the adsorbent from leaking.

2. The culture bottle with physical isolation according to claim 1, characterized in that: The anti-leakage portion is located at the upper end of the containing portion, and the minimum inner diameter of the anti-leakage portion is smaller than the minimum inner diameter of the containing portion.

3. The culture bottle with physical isolation according to claim 1, characterized in that: The embedded structure further includes a connecting portion located between the anti-leakage portion and the bottle mouth, and the connecting portion enables the embedded structure to be suspended in the culture bottle body.

4. The culture bottle with physical isolation according to claim 3, characterized in that: The upper end of the connecting portion is engaged between the bottle mouth and the bottle mouth plug.

5. The culture bottle with physical isolation according to claim 3, characterized in that: The connecting portion is cylindrical and is provided with a communicating hole that passes through the inside and outside of the embedded structure.

6. The culture bottle with physical isolation according to claim 5, characterized in that: There are a plurality of communicating holes, and the communicating holes are distributed around the connecting portion.

7. The culture bottle with physical isolation according to claim 6, characterized in that: The communicating holes are arranged in a row around the connecting portion, and the diameter of the communicating holes is larger than the inner diameter of the anti-leakage portion.

8. The culture bottle with physical isolation according to claim 6, characterized in that: The communication holes are arranged in multiple rows around the connecting portion, and the diameter of the communication holes is smaller than the inner diameter of the anti-leakage portion.

9. The culture bottle with physical isolation according to any one of claims 5 to 8, characterized in that: An embedded bottle stopper is provided in the embedded structure, and the embedded bottle stopper is used to seal the anti-leakage part.

10. The culture bottle with physical isolation according to claim 9, characterized in that: The height from the lower end of the bottle mouth plug to the upper end of the communicating hole is greater than the height of the embedded bottle plug, or the inner diameter of the connecting portion at the communicating hole is greater than the outer diameter of the embedded bottle plug.

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