Cell culture device

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture device which comprises a culture box, a rotating table is arranged at one end of the culture box, a rotating seat is rotatably connected to the rotating table through a sliding rail and a pulley, a mounting groove with a hollow center is formed in the rotating seat, and a culture cylinder is mounted through the mounting groove; a piston is arranged in the culture cylinder, a hydraulic cylinder in driving connection with the piston is mounted at the bottom of the culture cylinder, and a layered sampling cylinder cover is in threaded connection with the top of the culture cylinder. According to the utility model, the sampling tube is nested and inserted with the flow guide tube interface, the hydraulic cylinder is controlled to work to drive the piston to move upwards, the culture solution is driven to move upwards by one layer in the culture cylinder, and the electric telescopic rod is utilized to drive the rubber arc-shaped scraper blade to separate the layered liquid above and guide the liquid into the sampling tube from the flow guide tube interface, and the liquid is drained into the sampling bottle through the drainage tube; the sampling is carried out in a closed environment, and the sampling layering treatment is thorough and rapid.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture device. Background Art

[0002] Chinese patent application number CN202010741163.0 discloses a culture device for automated animal cell amplification in vaccine production, comprising a circular disc; the disc includes an annular cover plate, a bottom wall, and a side wall extending along the outer edge of the bottom wall; the inner side of the bottom wall is sequentially provided with a culture fluid collection tank, a diversion slope, and a culture plane from the outer edge to the center; wherein the side wall, bottom wall, and diversion slope form a culture fluid collection tank; a liquid collection hole is provided at the bottom of the culture fluid collection tank; the bottom end of the diversion slope is connected to the culture plane; the annular cover plate is sealed to the side wall, and the inner diameter is the same as the outer diameter of the culture plane. The culture device of the present invention has a simple structure and can be used for automated animal cell amplification in vaccine production.

[0003] However, after cells are cultured in culture medium, several different layers are produced by centrifugation. To remove the cell cluster or culture medium layer by layer, a pipette or a pipette is generally used, which means that the sample is taken with the lid open. Moreover, the sampling method makes the sampling slow and incomplete.

[0004] Taking samples with the lid open will cause temperature loss inside the culture tube, and viruses or bacteria from the outside will enter the culture tube, seriously affecting the quality of cell culture. For this purpose, a cell culture device is designed. Summary of the Invention

[0005] In order to solve the problems raised in the above background technology, the utility model provides a cell culture device, which controls the hydraulic cylinder to drive the piston to move upward, driving the culture fluid to move one layer upward in the culture tube, and uses the electric telescopic rod to drive the rubber arc scraper to separate the liquid in the upper layer and guide it from the guide tube interface into the sampling tube, and then drain it into the sampling bottle through the drainage tube; until all the culture fluid layers are sampled and classified into the sampling bottle, so that sampling is carried out in a closed environment, and the sampling layer processing is thorough and fast.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a cell culture device, comprising an incubator, a rotating platform is provided on one end of the incubator, a rotating seat is provided on the rotating platform through a slide rail and a pulley for rotation connection, a centrally hollowed mounting groove is provided inside the rotating seat, and a culture cylinder is installed through the mounting groove, a motor is installed on the incubator, a driving gear is provided at the driving end of the motor for meshing with the rotating seat, a piston is provided inside the culture cylinder, a hydraulic cylinder driven by the piston is installed at the bottom of the culture cylinder, a layered sampling cylinder cover is threadedly connected to the top of the culture cylinder, a guide tube interface is provided at the upper end of one side of the culture cylinder, and a sampling structure is plugged and connected through the guide tube interface.

[0007] As a preferred solution, an electric telescopic rod is installed on one end of the stratified sampling tube cover. The telescopic end of the electric telescopic rod penetrates into the interior of the stratified sampling tube cover and is connected to a rubber arc scraper via a connecting rod. The rubber arc scraper and the guide tube interface are on the same horizontal plane. The rubber arc scraper is driven by the electric telescopic rod to move horizontally inside the upper port of the culture tube.

[0008] As a preferred solution, a gear ring is provided on the outer wall of the rotating seat and is meshed with the driving gear. The rotating seat is driven by a motor to rotate on the rotating table via a pulley, thereby driving the culture cylinder to rotate.

[0009] As a preferred solution, the sampling structure includes a sampling bottle and a sampling tube. One end of the sampling tube passes through the partition inside the incubator and is detachably nested with the guide tube interface. The other end of the sampling tube is connected to the sampling bottle through a three-way valve, the drainage tube and the bottle cap.

[0010] As a preferred solution, the incubator is divided into a culture chamber and a sampling chamber by a partition, the top of the culture chamber is connected to an operation box cover, the front end face of the culture chamber is connected to an observation door, and the sampling chamber is connected to a sampling box door.

[0011] As a preferred solution, the incubator is provided with an electric control box electrically connected to the motor, the hydraulic cylinder and the electric telescopic rod, and the electric control box is provided with a switch panel.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] In the cell culture process, the utility model utilizes a motor to drive the rotating seat to drive the culture tube to rotate, and the cell culture situation inside the culture tube can be easily observed through the observation door.

[0014] After the cells are cultured with culture medium, the cell groups or culture medium layers need to be taken out in layers. The sampling box door is opened, and the sampling tube and the guide tube interface are nested and plugged into each other. The hydraulic cylinder is controlled to drive the piston inside the culture tube to move slowly upward, driving the culture medium to move up one layer in the culture tube. After reaching the upper port, the electric telescopic rod is used to drive the rubber arc scraper to separate the liquid in the upper layer and guide it from the guide tube interface into the sampling tube, and then drained into a sampling bottle through one of the drainage tubes; the above operation process is repeated many times until all layers of the culture medium are taken out from the culture tube, and the classified samples are stored in the classified sampling bottles, so that the sampling is carried out in a closed environment, and the sampling layer processing is thorough and fast; it prevents external viruses or bacteria from entering the culture tube and seriously affecting the cell culture quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2It is a schematic diagram of the internal three-dimensional structure of the utility model;

[0017] Figure 3 This is a schematic diagram of the other side of the three-dimensional structure of the interior of the utility model;

[0018] Figure 4 for Figure 2 Schematic diagram of the top view structure;

[0019] Figure 5 for Figure 4 Schematic diagram of the cross-sectional structure of the AA surface in the middle.

[0020] Description of Figure Numbers:

[0021] 1. Incubator; 2. Rotating table;

[0022] 3. Rotating seat; 31. Pulley;

[0023] 4. Culture tube; 41. Flow tube interface; 42. Hydraulic cylinder; 43. Piston;

[0024] 5. Layered sampling cylinder cover; 51. Electric telescopic rod; 52. Rubber curved scraper;

[0025] 6. Sampling bottle; 61. Bottle cap; 62. Drainage tube;

[0026] 7. Three-way valve; 71. Sampling tube;

[0027] 8. Motor; 9. Driving gear;

[0028] 11. Observation door; 12. Operation box cover; 13. Sampling box door; 14. Partition. DETAILED DESCRIPTION

[0029] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention. Example

[0030] See also Figure 1-5The utility model provides the following technical solutions: a cell culture device, including an incubator 1, a rotating platform 2 is provided on one end of the incubator 1, a rotating seat 3 is provided on the rotating platform 2 and is rotatably connected to the rotating seat 3 through a slide rail via a pulley 31, a mounting groove with a central hollow is provided inside the rotating seat 3, and a culture cylinder 4 is installed through the mounting groove, a motor 8 is installed on the incubator 1, a driving end of the motor 8 is provided with a driving gear 9 that is meshed with the rotating seat 3, a piston 43 is provided inside the culture cylinder 4, and a hydraulic cylinder 42 is installed at the bottom of the culture cylinder 4 and is driven and connected to the piston 43 The top of the culture cylinder 4 is threadedly connected to a stratified sampling cylinder cover 5, and an upper end of one side of the culture cylinder 4 is provided with a guide tube interface 41, and a sampling structure is connected through the guide tube interface 41; an electric telescopic rod 51 is installed on one end of the stratified sampling cylinder cover 5, and the telescopic end of the electric telescopic rod 51 penetrates into the interior of the stratified sampling cylinder cover 5 and is connected to a rubber arc scraper 52 through a connecting rod. The rubber arc scraper 52 is in the same horizontal plane as the guide tube interface 41, and the rubber arc scraper 52 is driven by the electric telescopic rod 51 to move horizontally inside the upper port of the culture cylinder 4. After the cells are cultured with the culture medium, if you want to take out the cell group or culture medium layer in layers, at this time, open the sampling box door 13, nest the sampling tube 71 and the guide tube interface 41, control the hydraulic cylinder 42 to drive the piston inside the culture tube 4 to move slowly upward, and drive the culture medium to move upward one layer in the culture tube 4. After reaching the upper end, use the electric telescopic rod 51 to drive the rubber arc scraper 52 to separate the liquid in the upper layer from the guide tube interface 41 and guide it into the sampling tube 71, and then drain it into a sampling bottle 6 through one of the drainage tubes 62; repeat the above operation process many times until all layers of the culture medium are taken out from the culture tube 4, and the classified samples are stored in the classified sampling bottles 6, so that the sampling is carried out in a closed environment, and the sampling layering processing is thorough and fast; it prevents external viruses or bacteria from entering the culture tube and seriously affecting the culture quality of the cells.

[0031] In this embodiment, a gear ring is provided on the outer wall of the rotating base 3, which is meshed with the driving gear 9. The rotating base 3 is driven by the motor 8 to rotate on the rotating table 2 via the pulley 31, thereby driving the culture tube 4 to rotate; the motor 8 can be started to drive the rotating base 3 via the driving gear 9, thereby driving the culture tube 4 to rotate, and the cell culture status inside the culture tube 4 can be easily observed through the observation door 11.

[0032] In this embodiment, the sampling structure includes a sampling bottle 6 and a sampling tube 71. One end of the sampling tube 71 passes through the partition 14 inside the incubator 1 and is detachably nested with the guide tube interface 41. The other end of the sampling tube 71 is connected to the sampling bottle 6 through the three-way valve 7, the drainage tube 62 and the bottle cap 61; the guide tube interface 41 and the sampling tube 71 are in a downward tilted state.

[0033] In this embodiment, the incubator 1 is divided into a culture chamber and a sampling chamber by a partition 14. The top of the culture chamber is connected to an operation box cover 12, the front end face of the culture chamber is connected to an observation door 11, and the sampling chamber is connected to a sampling box door 13.

[0034] In this embodiment, the incubator 1 is provided with an electric control box electrically connected to the motor 8 , the hydraulic cylinder 42 , and the electric telescopic rod 51 , and the electric control box is provided with a switch panel.

[0035] In addition, the culture tube 4 is designed with a transparent material, which makes it easy to observe the cell culture conditions inside.

[0036] The working principle and usage process of the present invention are as follows: when using the present invention, the operating box cover 12 is opened, the stratified sampling tube cover 5 is opened, cells are cultured inside the culture tube 4 using culture medium, and the stratified sampling tube cover 5 is closed. During the cell culture process, the motor 8 can be started to drive the rotating seat 3 through the driving gear 9 to drive the culture tube 4 to rotate, and the cell culture situation inside the culture tube 4 can be easily observed through the observation door 11.

[0037] After the cells are cultured with the culture medium, if you want to take out the cell group or culture medium layer in layers, at this time, open the sampling box door 13, nest the sampling tube 71 and the guide tube interface 41, control the hydraulic cylinder 42 to drive the piston inside the culture tube 4 to move slowly upward, and drive the culture medium to move upward one layer in the culture tube 4. After reaching the upper end, use the electric telescopic rod 51 to drive the rubber arc scraper 52 to separate the liquid in the upper layer from the guide tube interface 41 and guide it into the sampling tube 71, and then drain it into a sampling bottle 6 through one of the drainage tubes 62; repeat the above operation process many times until all layers of the culture medium are taken out from the culture tube 4, and the classified samples are stored in the classified sampling bottles 6, so that the sampling is carried out in a closed environment, and the sampling layering processing is thorough and fast; it prevents external viruses or bacteria from entering the culture tube and seriously affecting the culture quality of the cells.

[0038] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A cell culture device, comprising an incubator (1), characterized in that: The incubator (1) is provided with a rotating platform (2) at one end, and a rotating seat (3) is provided on the rotating platform (2) for rotational connection via a pulley (31) through a slide rail, and a centrally hollowed mounting groove is provided inside the rotating seat (3), and a culture cylinder (4) is installed through the mounting groove, and a motor (8) is installed on the incubator (1), and a driving gear (9) is provided at the driving end of the motor (8) for meshing connection with the rotating seat (3), and a piston (43) is provided inside the culture cylinder (4), and a hydraulic cylinder (42) is provided at the bottom of the culture cylinder (4) for driving connection with the piston (43), and a layered sampling cylinder cover (5) is threadedly connected to the top of the culture cylinder (4), and a guide pipe interface (41) is provided at the upper end of one side of the culture cylinder (4), and a sampling structure is plugged and connected through the guide pipe interface (41).

2. The cell culture device according to claim 1, wherein: An electric telescopic rod (51) is installed on one end of the stratified sampling tube cover (5). The telescopic end of the electric telescopic rod (51) penetrates into the stratified sampling tube cover (5) and is connected to a rubber arc scraper (52) via a connecting rod. The rubber arc scraper (52) and the guide tube interface (41) are in the same horizontal plane. The rubber arc scraper (52) is driven by the electric telescopic rod (51) to move horizontally inside the upper port of the culture tube (4).

3. The cell culture device according to claim 1, wherein: A gear ring is provided on the outer wall of the rotating seat (3) and is meshed with the driving gear (9). The rotating seat (3) is driven by the motor (8) to rotate on the rotating table (2) via the pulley (31), thereby driving the culture cylinder (4) to rotate.

4. The cell culture device according to claim 1, wherein: The sampling structure comprises a sampling bottle (6) and a sampling tube (71). One end of the sampling tube (71) passes through the partition (14) inside the incubator (1) and is detachably nested and plugged into the drainage tube interface (41). The other end of the sampling tube (71) is connected to the sampling bottle (6) through the drainage tube (62) and the bottle cap (61) via the three-way valve (7).

5. The cell culture device according to claim 1, wherein: The incubator (1) is divided into a culture chamber and a sampling chamber by a partition (14); the top of the culture chamber is connected to an operation box cover (12); the front end surface of the culture chamber is connected to an observation door (11); and the sampling chamber is connected to a sampling box door (13).

6. The cell culture device according to claim 1, wherein: The incubator (1) is provided with an electric control box electrically connected to the motor (8), the hydraulic cylinder (42), and the electric telescopic rod (51), and the electric control box is provided with a switch panel.

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