Ultrafiltration concentration tube device with sterile filtration function

Abstract

The utility model provides an ultrafiltration concentration tube device with a sterile filtration function, which solves the problems that the existing ultrafiltration tubes with different specifications and models can only achieve the purpose of protein concentration, different proteins can be confused due to frequent operation after the concentration is finished, the recovery rate is reduced, the risk of introducing bacteria is increased and the like. According to the main scheme, a tube body internally provided with a cavity is included, the top of the tube body is open, the tube body can be sealed into a whole through a tube cover, the tube body is formed by splicing three sections, the adjacent splicing positions are fixed through deformation friction, and the three sections of the tube body sequentially comprise an antibody storage area, a liquid storage area and a liquid discharging area from the upper end opening to the lower end opening; a first supporting table and a second supporting table are fixed to the two sequentially-connected positions of the inner wall of the pipe body respectively, a sterile filter membrane is embedded in the first supporting table, and an ultrafiltration membrane is embedded in the second supporting table.

Description

Technical Field

[0001] The utility model relates to the technical field of protein extraction, in particular to an ultrafiltration concentration tube device with sterile filtration. Background Art

[0002] Ultrafiltration tubes are simple to use for concentrating various proteins / antibodies, with high recovery rates, fewer steps, easy-to-understand operation methods, high throughput, and a short processing time. Therefore, they are widely used in CRO services and early purification research and development to concentrate and replace small-volume proteins and increase protein concentration.

[0003] Typically, there are various ultrafiltration tubes with different molecular weights available, including membrane cutoffs of 3KD, 10KD, and 30KD. Each specification also has volume specifications of 1.5ml, 5ml, and 25ml, suitable for concentrating proteins of different molecular weights and volumes. The appropriate membrane cutoff and volume specification can be selected based on the molecular weight of the target protein and the volume to be concentrated.

[0004] To ensure sterility, the concentrated protein must be removed after ultrafiltration and sterile filtered using syringes of varying sizes connected to a 0.22 μm sterile filter to prevent bacterial growth during storage. Each additional step reduces recovery and increases the risk of bacterial introduction. To address this issue, we propose an ultrafiltration concentrator device with sterile filtration to address this issue. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the shortcomings of the existing technology. This utility model provides an ultrafiltration and concentrating tube device with sterile filtration. It can achieve the goals of sterile filtration and protein concentration simultaneously through a single centrifugation, eliminating the need for repeated protein extraction and filtration. This improves recovery rate and saves time.

[0006] In order to solve the above technical problems, the technical solution adopted by the utility model is: an ultrafiltration concentration tube device with sterile filtration, comprising: a tube body with a cavity inside, the top of the tube body is open and can be sealed as a whole by a tube cover, the tube body is spliced ​​into three sections and the adjacent spliced ​​parts are fixed by deformation friction, the three sections of the tube body include an antibody storage area, a liquid storage area and a lower liquid area from the upper end opening to the bottom, and the inner wall of the tube body is fixed with a first support platform and a second support platform at two sequentially connected places, the first support platform is embedded with a sterile filter membrane, and the second support platform is embedded with an ultrafiltration membrane.

[0007] Furthermore, two ends of the second support platform are provided with embedding grooves for accommodating the ultrafiltration membrane, and a liquid port with a trapezoidal cross-section protrudes downward from the center. The top of the liquid port is connected to the two embedding grooves respectively, and the bottom of the embedding groove is connected to the lower liquid area.

[0008] Furthermore, the ultrafiltration membrane comprises two sheets, which are in the shape of square sheets and are symmetrically thermoplastic welded in each embedding groove. The size of the ultrafiltration membrane is 2*1.5 cm.

[0009] Furthermore, the interval size of the liquid ports gradually narrows from top to bottom, and the interval range is 0.3-0.6 mm.

[0010] Furthermore, the sterile filter membrane is in the shape of a circular sheet with a diameter of 2.5 mm and a surface pore diameter of 0.22 μm.

[0011] Furthermore, the ultrafiltration membrane includes multiple models, including 3KD, 10KD, and 30KD.

[0012] Furthermore, the outer wall of the antibody storage area is engraved with liquid volume scale.

[0013] Furthermore, the bottom end of the lower liquid area is a tapered opening for matching an external centrifuge tube rack.

[0014] Compared with the prior art, the present invention has the following advantages: by using the sterile filtration membrane and ultrafiltration membrane arranged in layers above and below, the sample can be sterile filtered without further filtration. After concentration, the protein can be directly removed and stored in a sterile tube. This allows for simultaneous sterile filtration and protein concentration in a single centrifugation, eliminating the need for repeated protein removal and filtration. This improves recovery and saves extraction time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The disclosure of the present invention is described with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of the present invention. In the accompanying drawings, the same reference numerals are used to refer to the same components. Among them:

[0016] Figure 1 The figure schematically shows the overall structure of an embodiment of the present invention.

[0017] Numbers in the figure: 1. Tube body; 2. Antibody storage area; 3. Liquid storage area; 4. Lower liquid area; 5. First support platform; 6. Second support platform; 7. Sterile filter membrane; 8. Ultrafiltration membrane; 9. Embedded groove; 10. Liquid port; 11. Liquid volume scale; 12. Conical port; 13. Tube cover. DETAILED DESCRIPTION

[0018] It is easy to understand that according to the technical solution of the present invention, without changing the essential spirit of the present invention, a person skilled in the art can propose a variety of interchangeable structural methods and implementation methods. Therefore, the following specific embodiments and drawings are only illustrative of the technical solution of the present invention and should not be regarded as the entire present invention or as a limitation or restriction of the technical solution of the present invention.

[0019] According to one embodiment of the present invention, Figure 1 Shown.

[0020] In this embodiment, as for the overall structure, an ultrafiltration concentration tube device with sterile filtration includes: a tube body 1 with a cavity inside, the top of the tube body is open and can be sealed as a whole by a tube cover 13, the tube body 1 is spliced ​​into three sections and the adjacent spliced ​​parts are fixed by deformation friction, the three sections of the tube body 1 include an antibody storage area 2, a liquid storage area 3 and a lower liquid area 4 from the upper end opening to the bottom, and the inner wall of the tube body 1 is fixed with a first support platform 5 and a second support platform 6 at two sequentially connected places, a sterile filter membrane 7 is embedded on the first support platform 5, and an ultrafiltration membrane 8 is embedded on the second support platform 6.

[0021] The ultrafiltration membrane 8 comes in various sizes, including 3KD, 10KD, and 30KD. The second support 6 has two ends with recesses 9 (located in the inner layer, not shown) for accommodating the ultrafiltration membrane 8. A trapezoidal liquid inlet 10 protrudes downward from the center. The top of the liquid inlet 10 communicates with the two corresponding recesses 9, and the bottom of the recess 9 communicates with the lower liquid zone. The ultrafiltration membrane 8 comprises two square sheets symmetrically thermoplastically welded within each recess 9. The membrane measures 2 x 1.5 cm. The spacing between the liquid inlets 10 gradually narrows from top to bottom, ranging from 0.3 to 0.6 mm.

[0022] As for the sterile filter membrane 7, the sterile filter membrane 7 is a circular sheet with a diameter of 2.5 mm and a surface pore diameter of 0.22 um.

[0023] In combination with the above structural records, this solution filters the antibody liquid by adding a 0.22um sterile filter membrane 7 and an antibody storage area 2. The bacteria can be blocked by the filter holes on the surface, and then flow into the liquid port 10 under the action of gravity. The two connected grooves 9 at the top of the liquid port 10 will pre-thermoplastically weld two ultrafiltration membranes 8. The water molecules in the protein will be thrown out by the centrifugal force of the external centrifugal device, and fall into the lower liquid area 4 through the preset holes at the bottom of the groove 9, thereby achieving sterile filtration of the antibody protein. Similarly, the tube body 1 itself is designed with three sections, and each section can be disassembled to remove the required part. The trapezoidal cross-section of the liquid port 10 itself and the design of the gradually decreasing interval downward are designed to allow the last remaining liquid to be at the bottom of the liquid port 10 as much as possible, so that it can be easily sucked out by a pipette to reduce residue.

[0024] Furthermore, to facilitate liquid content measurement, the present invention also features liquid volume scale 11 engraved on the outer wall of the antibody storage area 2. Similarly, the outer wall of the liquid port 10 for placing the sample liquid may also have corresponding scale lines (not shown). Similarly, the bottom of the lower liquid area 4 features a tapered opening 12 for accommodating an external centrifuge tube rack, making the sample placement process more stable and less prone to shaking.

[0025] The technical scope of the present invention is not limited to the contents described above. Those skilled in the art can make various deformations and modifications to the above embodiments without departing from the technical concept of the present invention, and these deformations and modifications should all fall within the scope of protection of the present invention.

Claims

1. An ultrafiltration concentration tube device with sterile filtration, characterized in that: include: The tube body has a cavity inside, the top of the tube body is open and can be sealed as a whole by a tube cover. The tube body is spliced ​​into three sections and the adjacent joints are fixed by deformation friction. The three sections of the tube body include an antibody storage area, a liquid storage area and a lower liquid area from the upper end opening to the bottom, and the inner wall of the tube body is fixed with a first support platform and a second support platform at two sequentially connected places. A sterile filter membrane is embedded on the first support platform, and an ultrafiltration membrane is embedded on the second support platform.

2. The ultrafiltration and concentration tube device with sterile filtration according to claim 1, characterized in that: The second support platform has embedding grooves at both ends for accommodating the ultrafiltration membrane, and a liquid port with a trapezoidal cross-section protrudes downward from the center. The top of the liquid port is connected to the two embedding grooves respectively, and the bottom of the embedding groove is connected to the lower liquid area.

3. The ultrafiltration and concentration tube device with sterile filtration according to claim 2, characterized in that: The ultrafiltration membrane comprises two sheets, which are in the shape of square sheets and are symmetrically thermoplastic welded in each embedding groove. The size of the ultrafiltration membrane is 2*1.5 cm.

4. The ultrafiltration and concentration tube device with sterile filtration according to claim 2, characterized in that: The liquid port spacing gradually narrows from top to bottom, and the spacing range is 0.3-0.6 mm.

5. The ultrafiltration and concentration tube device with sterile filtration according to claim 1, characterized in that: The sterile filter membrane is in the shape of a circular sheet with a diameter of 2.5 mm and a surface pore diameter of 0.22 μm.

6. The ultrafiltration and concentration tube device with sterile filtration according to claim 1, characterized in that: The ultrafiltration membrane models include multiple types, including 3KD, 10KD, and 30KD.

7. The ultrafiltration and concentration tube device with sterile filtration according to claim 1, characterized in that: The outer wall of the antibody storage area is also engraved with liquid volume scales.

8. The ultrafiltration and concentration tube device with sterile filtration according to claim 1, characterized in that: The bottom end of the lower liquid area is a tapered opening for matching with an external centrifuge tube rack.

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