Full-automatic flaky carrier cell culture and digestion reaction device

Abstract

The utility model discloses a full-automatic flaky carrier cell culture and digestion reaction device which comprises a reactor loaded with a flaky carrier, a cell collection container, a first liquid storage container, a second liquid storage container, a third liquid storage container, a fourth liquid storage container, a fifth liquid storage container, a sixth liquid storage container, a seventh liquid storage container and a controller, the cell collection container, the first liquid storage container, the second liquid storage container, the third liquid storage container, the fourth liquid storage container, the fifth liquid storage container, the sixth liquid storage container and the seventh liquid storage container are all connected with the reactor, and the reactor is electrically connected with the controller. The device disclosed by the utility model has the advantages of full-automatic operation, small batch difference and high cell recovery rate.

Description

Technical Field

[0001] The utility model relates to the technical field of cell engineering, in particular to a full-automatic reaction device for culturing and digesting sheet-shaped carrier cells. Background Art

[0002] In recent years, with increasing market demand for vaccines and therapeutic protein-based biologics and stringent quality requirements, the application of large-scale cell culture technology has highlighted its safety, cost-effectiveness, and effectiveness. Since mammalian cells have long been used as carriers for the production of therapeutic proteins, they have been widely used in the biopharmaceutical industry. Sheet-shaped carriers are highly efficient and specifically designed for adherent mammalian cell culture. They provide optimal culture conditions for adherent growth, enabling the establishment of suitable large-scale culture systems for high-density cell culture and efficient product expression. Consequently, they are currently widely used in conjunction with fixed-bed bioreactors to achieve scale-up of cell culture. However, the current process for scale-up of cell culture using fixed-bed bioreactors is often manual, subject to significant operational errors. This is because cells are generally very sensitive to culture conditions, particularly during the digestion process, which are sensitive to factors such as pH, temperature, pancreatin concentration, pancreatin duration, and shear stress. Even the slightest inaccuracy can lead to significant errors. Since manual operations need to be completed by humans, humans will inevitably encounter times when they are slow to respond and lack timeliness, thus missing the optimal operating point, resulting in errors in the results and unsatisfactory cell recovery results.

[0003] Furthermore, existing fixed-bed bioreactors often utilize basket-type fixed beds, and the harvesting tube used to harvest cells is located relatively close to the inner wall of the fixed-bed bioreactor tank, typically resting snugly against the tank wall. Fixed-bed bioreactors with this structural configuration generally suffer from the following issues: When the agitator paddles rotate, the liquid within the fixed-bed bioreactor carries the flaky carriers with it as it rotates within the basket-type fixed bed. When encountering various obstructions, this affects the mixing of the medium (i.e., the flaky carriers) within the tank, causing the medium to become stuck between the obstruction (e.g., the cell harvesting tube) and the tank wall, or sink to the bottom. This in turn affects the efficiency of the agitator paddles in pumping the cells onto the flaky carriers, thereby affecting the final cell harvest rate. Utility Model Content

[0004] The purpose of the utility model is to provide a fully automatic reaction device for culturing and digesting sheet-like carrier cells, so as to solve the technical problems existing in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0006] A fully automatic reaction device for culturing and digesting sheet-like carrier cells, comprising a reactor loaded with sheet-like carriers, a cell collection container, a first liquid storage container, a second liquid storage container, a third liquid storage container, a fourth liquid storage container, a fifth liquid storage container, a sixth liquid storage container, a seventh liquid storage container, and a controller, wherein the cell collection container, the first liquid storage container, the second liquid storage container, the third liquid storage container, the fourth liquid storage container, the fifth liquid storage container, the sixth liquid storage container, and the seventh liquid storage container are all connected to the reactor, and the reactor is electrically connected to the controller;

[0007] Among them, the reactor is used for cell culture and digestion; the cell collection container is used to collect cells that have been cultured and digested in the reactor; the first liquid storage container is used to store cell suspension and provide cell suspension to the reactor; the second liquid storage container is used to collect liquid discharged from the reactor; the third liquid storage container is used to store trypsin digestion solution and provide trypsin digestion solution to the reactor; the fourth liquid storage container is used to store PBS buffer and provide PBS buffer to the reactor; the fifth liquid storage container is used to store stop solution and provide stop solution to the reactor; the sixth liquid storage container is used to store nutrient solution and provide nutrient solution to the reactor; the seventh liquid storage container is used to store waste liquid discharged from the reactor; the controller is used to control the first liquid storage container, the third liquid storage container, the fourth liquid storage container, the fifth liquid storage container and the sixth liquid storage container to automatically transport corresponding cell suspension, trypsin digestion solution, PBS buffer, stop solution and nutrient solution to the reactor, and control the reactor to automatically perform cell culture and digestion operations, and after the cell culture is completed, control the cell collection container to automatically collect cells.

[0008] Furthermore, the cell collection container, the first liquid storage container, and the second liquid storage container are all connected to the reactor via a first pipeline; the third liquid storage container, the fourth liquid storage container, and the fifth liquid storage container are all connected to the reactor via a second pipeline; the sixth liquid storage container is connected to the reactor via a third pipeline; and the seventh liquid storage container is connected to the reactor via a fourth pipeline.

[0009] The first pipeline includes a main pipeline A and three branch pipelines A, wherein the main pipeline A is connected to the reactor, and one end of the three branch pipelines A is connected to the main pipeline A, and the other end is connected to the cell collection container, the first liquid storage container, and the second liquid storage container;

[0010] The second pipeline includes a main pipeline B and three branch pipelines B, the main pipeline B is connected to the reactor, and one end of the three branch pipelines B is connected to the main pipeline B, and the other end is connected to the third liquid storage container, the fourth liquid storage container, and the fifth liquid storage container;

[0011] Wherein, peristaltic pumps are provided on the three branch pipelines A of the first pipeline, the three branch pipelines B of the second pipeline, the third pipeline and the fourth pipeline, and the peristaltic pumps are electrically connected to the controller.

[0012] Furthermore, the reactor includes a tank body and a tank cover arranged on the top of the tank body, a magnetic stirrer is arranged on the top of the tank cover, a stirring paddle shaft and a basket paddle base are arranged inside the tank body, the upper end of the stirring paddle shaft is connected to the output end of the magnetic stirrer, and the lower end is passed through the inside of the basket paddle base, the basket paddle base is supported on the bottom wall of the tank body, and the magnetic stirrer is electrically connected to the controller.

[0013] Furthermore, a DO electrode, a PH electrode and a sampling tube are installed on the tank cover, and the control parts of the DO motor and the PH electrode are located above the tank cover and electrically connected to the controller. The detection parts are extended inside the tank body for contacting the liquid in the tank body. One end of the sampling tube is extended above the tank cover, and the other end passes through the tank cover and extends into the tank body for contacting the liquid in the tank body.

[0014] Furthermore, a harvest tube is provided inside the tank body, and the upper end of the harvest tube passes through the tank cover and extends above the tank cover, and is used to communicate with the cell collection container, the first liquid storage container and the second liquid storage container. The lower end of the harvest tube passes through the basket paddle base and is connected to the inner cavity of the tank body at the lower part of the basket paddle base. There is a gap of at least 5CM between the harvest tube and the inner wall of the tank body.

[0015] Furthermore, the agitator shaft includes a agitator main shaft, a agitator connecting pipe, a agitator top cover, a agitator drainage chamber, a agitator shaft guide pipe and a agitator shaft small blade. The upper end of the agitator main shaft is connected to the output end of the magnetic stirrer, and the lower end is connected to the agitator connecting pipe, the agitator connecting pipe is connected to the agitator top cover, the agitator top cover is connected to the agitator drainage chamber, the agitator drainage chamber is connected to the agitator shaft guide pipe, the agitator shaft guide pipe passes through the basket paddle base and extends into the tank body cavity at the lower part of the basket paddle base. There are several agitator shaft small blades, which are annularly inclined and surround the outer peripheral wall of the agitator shaft guide pipe and are located inside the basket paddle base.

[0016] Furthermore, a plurality of stirring paddle discharge pipes are provided around the stirring paddle drainage cavity, and each stirring paddle discharge pipe is located on the upper part of the basket paddle base, and one end is connected to the stirring paddle drainage cavity, and the other end is connected to the inner cavity of the tank body of the basket paddle base.

[0017] Furthermore, a stirring paddle shaft ventilation ring tube is also surrounded on the outer peripheral wall of the stirring paddle shaft guide tube; a stirring paddle shaft central air pipe is also provided inside the stirring paddle shaft guide tube; one end of the stirring paddle shaft ventilation ring tube is arranged on the outer wall of the stirring paddle shaft guide tube and is connected to the inner cavity of the basket paddle base, and the other end is connected to the stirring paddle shaft central air pipe, and the stirring paddle shaft central air pipe is connected to the external atmosphere.

[0018] Furthermore, the basket paddle base includes a porous bottom plate and a liquid guiding tube. A plurality of base pillars are provided on the outer tube wall at the lower end of the liquid guiding tube. The porous bottom plate is sleeved on the outside of the liquid guiding tube and fixed on the inner wall of the tank body. The base pillars are supported on the bottom wall of the tank body.

[0019] Furthermore, a water jacket is provided on the outside of the tank body, a support is provided at the lower part of the water jacket, the water jacket is fixedly supported on the support, a water inlet and a water outlet are provided on the support, and the water inlet and the water outlet are both connected to the water jacket.

[0020] Compared with the prior art, the advantages of the present invention are as follows:

[0021] (1) Sensitive response and small error. Since the device adopts fully automatic control, the entire cell culture and digestion process will automatically run according to the pre-set program of the device, and will not be affected by human factors. The whole process is sensitive and timely, effectively reducing operational errors. During batch production, the error between batches is small.

[0022] (2) High cell viability and harvest rate. Since there is a relatively large distance between the harvest tube and the inner wall of the reaction tank, the accumulation of sheet carriers in the cell harvest tube of the bioreactor is avoided. At the same time, the upper cover of the basket paddle base is omitted, and the sheet carriers can flow fully in the tank with the liquid in the tank under the action of the stirring paddle, thereby ensuring sufficient mass and oxygen transfer of the cells, thereby greatly improving the cell survival rate and harvest rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solution in this embodiment, the following is a brief introduction to the drawings required for the description of the embodiment. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 This is a schematic diagram of a fully automatic sheet-like carrier cell culture and digestion reaction device provided by the present invention;

[0025] Figure 2 is a schematic diagram of the internal structure of the reactor (without the sheet carrier);

[0026] Figure 3 It is a schematic diagram of the internal structure of the reactor (loaded with a sheet carrier);

[0027] Figure 4 It is a schematic diagram of the local structure of the reactor;

[0028] Figure 5 It is a structural diagram of the stirring paddle shaft;

[0029] Figure 6 It is a structural diagram of the basket paddle base;

[0030] Explanation of the reference numerals: 1, reactor; 101, tank cover; 102, tank body; 103, magnetic stirrer; 104, stirring paddle shaft; 104a, stirring paddle main shaft; 104b, stirring paddle connecting pipe; 104c, stirring paddle top cover; 104d, stirring paddle liquid discharge chamber; 104e, stirring paddle discharge pipe; 104f, stirring paddle shaft flow guide pipe; 104g, stirring paddle shaft small blade; 104h, stirring paddle shaft ventilation ring pipe; 104i, stirring paddle shaft central air pipe; 105, basket paddle base; 105a, porous bottom plate; 105b, liquid guide tube; 105c, base support Column; 106, DO electrode; 107, pH electrode; 108, sampling tube; 109, harvesting tube; 110, water jacket; 111, support; 112, water inlet; 113, water outlet; 2, cell collection container; 3, first liquid storage container; 4, second liquid storage container; 5, third liquid storage container; 6, fourth liquid storage container; 7, fifth liquid storage container; 8, sixth liquid storage container; 9, seventh liquid storage container; 10, first pipeline; 11, second pipeline; 12, third pipeline; 13, fourth pipeline; 14, peristaltic pump; 15, air filter; 16, sheet carrier. DETAILED DESCRIPTION

[0031] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the following further describes how the present invention is implemented in conjunction with the accompanying drawings and specific implementation methods.

[0032] See Figure 1 As shown, the present invention provides a fully automatic sheet carrier cell culture and digestion reaction device, comprising a reactor 1 loaded with a sheet carrier 16, a cell collection container 2, a first liquid storage container 3, a second liquid storage container 4, a third liquid storage container 5, a fourth liquid storage container 6, a fifth liquid storage container 7, a sixth liquid storage container 8, a seventh liquid storage container 9 and a controller (not shown in the figure), the cell collection container 2, the first liquid storage container 3, the second liquid storage container 4, the third liquid storage container 5, the fourth liquid storage container 6, the fifth liquid storage container 7, the sixth liquid storage container 8 and the seventh liquid storage container 9 are all connected to the reactor 1, and the reactor 1 is electrically connected to the controller;

[0033] Among them, the reactor 1 is used for cell culture and digestion; the cell collection container 2 is used to collect cells cultured and digested in the reactor 1; the first liquid storage container 3 is used to store cell suspension and provide cell suspension to the reactor 1; the second liquid storage container 4 is used to collect liquid discharged from the reactor 1; the third liquid storage container 5 is used to store trypsin digestion solution and provide trypsin digestion solution to the reactor 1; the fourth liquid storage container 6 is used to store PBS buffer and provide PBS buffer to the reactor 1; the fifth liquid storage container 7 is used to store stop solution and provide stop solution to the reactor 1; the sixth liquid storage container 8 is used to store nutrient solution and provide nutrient solution to the reactor 1; the seventh liquid storage container 9 is used to store waste liquid discharged from the reactor 1; the controller is used to control the first liquid storage container 3, the third liquid storage container 5, the fourth liquid storage container 6, the fifth liquid storage container 7 and the sixth liquid storage container 8 to automatically transport the corresponding cell suspension, trypsin digestion solution, PBS buffer, stop solution and nutrient solution to the reactor 1, and control the reactor 1 to automatically perform cell culture and digestion operations, and after the cell culture is completed, control the cell collection container 2 to automatically collect cells.

[0034] Specifically, as an embodiment of the present invention: the cell collection container 2, the first liquid storage container 3 and the second liquid storage container 4 are all connected to the reactor 1 through the first pipeline 10; the third liquid storage container 5, the fourth liquid storage container 6 and the fifth liquid storage container 7 are all connected to the reactor 1 through the second pipeline 11; the sixth liquid storage container 8 is connected to the reactor 1 through the third pipeline 12; the seventh liquid storage container 9 is connected to the reactor 1 through the fourth pipeline 13;

[0035] The first pipeline 10 includes a main pipeline A and three branch pipelines A. The main pipeline A is connected to the reactor 1. One end of the three branch pipelines A is connected to the main pipeline A, and the other end is connected to the cell collection container 2, the first liquid storage container 3, and the second liquid storage container 4.

[0036] The second pipeline 11 includes a main pipeline B and three branch pipelines B. The main pipeline B is connected to the reactor 1. One end of the three branch pipelines B is connected to the main pipeline B, and the other end is connected to the third liquid storage container 5, the fourth liquid storage container 6, and the fifth liquid storage container 7.

[0037] Among them, peristaltic pumps 14 are provided on the three branch pipes A of the first pipe 10, the three branch pipes B of the second pipe 11, the third pipe 12 and the fourth pipe 13, and the peristaltic pumps 14 are electrically connected to the controller; when working, the controller controls the peristaltic pumps 14 to work, and the peristaltic pumps 14 control the corresponding collection containers and liquid storage containers to be turned on and off.

[0038] Specifically, as an embodiment of the present invention: Figures 2 to 6As shown, the reactor 1 includes a tank body 102 and a tank cover 101 arranged on the top of the tank body 102, a magnetic stirrer 103 is arranged on the top of the tank cover 101, and a stirring paddle shaft 104 and a basket paddle base 105 are arranged inside the tank body 102. The upper end of the stirring paddle shaft 104 is connected to the output end of the magnetic stirrer 3, and the lower end is passed through the inside of the basket paddle base 105. The basket paddle base 105 is supported on the inner bottom wall of the tank body 102, and the magnetic stirrer 103 is electrically connected to the controller.

[0039] Specifically, as an embodiment of the present invention, a DO electrode 106, a pH electrode 107, and a sampling tube 108 are also mounted on the tank lid 101. The control units of the DO motor 106 and the pH electrode 107 are both located above the tank lid 101 and electrically connected to the controller. The detection units are both extended inside the tank body 102 for contact with the liquid within the tank body 102. One end of the sampling tube 108 extends above the tank lid 101, while the other end passes through the tank lid 101 and extends into the tank body 102 for contact with the liquid within the tank body 102. The DO electrode 106 is used to detect the dissolved oxygen content of the liquid within the tank body 102; the pH electrode 107 is used to detect the pH value of the liquid within the tank body 102; and the sampling tube 108 is used for sampling.

[0040] Specifically, as one embodiment of the present invention, a harvesting tube 109 is further disposed within the tank body 102. The upper end of the harvesting tube 109 penetrates the tank lid 101 and extends above the lid 101, communicating with the cell collection container 2, the first liquid storage container 3, and the second liquid storage container 4. The lower end of the harvesting tube 109 penetrates the basket paddle base 105 and communicates with the tank body cavity below the basket paddle base 105. A gap of at least 5 cm exists between the harvesting tube 109 and the inner wall of the tank body 102. The function of the harvesting tube 109 is to discharge cells cultured within the reactor 1.

[0041] Specifically, as an embodiment of the present invention, the stirring paddle shaft 104 includes a stirring paddle main shaft 104a, a stirring paddle connecting pipe 104b, a stirring paddle top cover 104c, a stirring paddle drainage chamber 104d, a stirring paddle shaft guide pipe 104e and a stirring paddle shaft small blade 104g, the upper end of the stirring paddle main shaft 104a is connected to the output end of the magnetic stirrer 103, the lower end is connected to the stirring paddle connecting pipe 104b, the stirring paddle connecting pipe 104b is connected to the stirring paddle top cover 104 c is connected, the stirring paddle top cover 104c is connected to the stirring paddle drainage chamber 104d, the stirring paddle drainage chamber 104d is connected to the stirring paddle shaft guide tube 104f, the stirring paddle shaft guide tube 104f passes through the basket paddle base 105, and extends to the inner cavity of the tank body at the lower part of the basket paddle base 105. There are several small stirring paddle shaft blades 104g, which are annularly inclined and surround the outer peripheral wall of the stirring paddle shaft guide tube 104f, and are located inside the basket paddle base 105.

[0042] Specifically, as one embodiment of the present invention, a plurality of paddle discharge pipes 104e are disposed around the paddle drainage chamber 104d. Each paddle discharge pipe 104e is located above the basket paddle base 105, with one end communicating with the paddle drainage chamber 104d and the other end communicating with the inner cavity of the tank body above the basket paddle base 105. The paddle discharge pipes 104e are used to discharge the liquid in the paddle drainage chamber 104d into the inner cavity of the tank body 102 under the action of centrifugal force.

[0043] Specifically, as an embodiment of the present invention: the outer peripheral wall of the agitator shaft guide tube 104f is also surrounded by a agitator shaft ventilation ring tube 104h; the inside of the agitator shaft guide tube 104f is also provided with a agitator shaft central air pipe 104i; one end of the agitator shaft ventilation ring tube 104h is arranged on the outer wall of the agitator shaft guide tube 104f and is connected to the inner cavity of the basket paddle base 105, and the other end is connected to the agitator shaft central air pipe 104i, and the agitator shaft central air pipe 104i is connected to the external atmosphere.

[0044] Specifically, as an embodiment of the present invention, the basket paddle base 105 includes a porous bottom plate 105a and a liquid guide tube 105b. The outer wall of the lower end of the liquid guide tube 105b is provided with a plurality of base supports 105c. The porous bottom plate 105a is sleeved over the liquid guide tube 105b and secured to the inner wall of the tank 102. The base supports 105c are supported on the inner bottom wall of the tank 102. The porous bottom plate 105a is used to intercept the sheet carrier 16.

[0045] Specifically, as an embodiment of the present invention, a water jacket 110 is provided on the outside of the tank body 101, a support 111 is provided at the lower part of the water jacket 110, the water jacket 110 is fixedly supported on the support 111, and a water inlet 112 and a water outlet 113 are provided on the support 111, and the water inlet 112 and the water outlet 113 are both connected to the water jacket 109. The water inlet 112 is used to allow water to enter the water jacket 110, and the water outlet 113 is used to discharge the water in the water jacket 110.

[0046] Specifically, as an embodiment of the present invention: the cell collection container 2, the first liquid storage container 3, the second liquid storage container 4, the third liquid storage container 5, the fourth liquid storage container 6, the fifth liquid storage container 7, the sixth liquid storage container 8 and the seventh liquid storage container 9 can be tank-type, barrel-type, bottle-type or bag-type collection containers.

[0047] Specifically, as an embodiment of the present invention: the cell collection container 2, the first liquid storage container 3, the second liquid storage container 4, the third liquid storage container 5, the fourth liquid storage container 6, the fifth liquid storage container 7, the sixth liquid storage container 8 and the seventh liquid storage container 9 are all provided with an air filter 15.

[0048] The working principle of the fully automatic sheet-like carrier cell culture and digestion reaction device provided by the utility model is as follows:

[0049] In the first step, the peristaltic pump 14 corresponding to the first liquid storage container 3 is started to transport the cell suspension in the first liquid storage container 3 through the first pipeline 10 and the harvesting tube 109 to the interior of the tank 102 of the reactor 1;

[0050] The second step is to start the magnetic stirrer 103 of the reactor 1, first drive the stirring paddle shaft 104 at a speed of 90 rpm through the magnetic stirrer 103, run for 10 minutes, and then adjust the speed of the stirring paddle shaft 104 to 60 rpm;

[0051] Step 3: Allow the cells to grow in the tank 102 for 5-7 days, during which time the sixth liquid storage container 8 automatically perfuses the tank 102 with nutrient solution, while the waste liquid is automatically discharged into the seventh liquid storage container 9.

[0052] Step 4: After the cells have grown in the tank 102 for 5-7 days, all the liquid in the tank 102 is discharged into the second liquid storage container 4;

[0053] Step 5: Start the peristaltic pump 14 corresponding to the fourth liquid storage container 6 to transfer the PBS buffer in the fourth liquid storage container 6 into the tank 102. Adjust the rotation speed of the stirring paddle shaft 104 to 90 rpm to wash the cells and sheet carriers in the tank 102 with the PBS buffer. After 5 minutes, drain all the waste liquid in the tank 102 into the second liquid storage container 4.

[0054] Step 6: Repeat step 5 to wash the cells and the sheet carrier in the tank 102 multiple times until the liquid in the tank 102 becomes transparent from turbidity.

[0055] Step 7: Start the peristaltic pump 14 corresponding to the third liquid storage container 5 to transfer the pancreatic digestion solution in the third liquid storage container 5 into the tank 102. The pancreatic digestion solution digests the cells on the sheet carrier. After N minutes (N is determined by whether the liquid in the tank 102 becomes turbid), the pancreatic digestion solution in the tank 102 is completely discharged into the second liquid storage container 4.

[0056] Step 8: Dry disinfection for 5-20 minutes;

[0057] In the ninth step, the peristaltic pump 14 corresponding to the fifth liquid storage container 7 is started to transfer the stop solution in the fifth liquid storage container 7 to the tank body 102, and the speed of the stirring paddle shaft 104 is adjusted to 300 rpm. After stirring for M minutes (M is set according to implementation needs), all the liquid in the tank body 102 is discharged into the cell collection container 2. At this point, the fully automatic control of the cell culture → digestion → harvesting is completed.

[0058] Finally, it should be noted that the above description is only an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A fully automatic cell culture and digestion reaction device for sheet-like carriers, comprising a reactor (1) loaded with sheet-like carriers (16), characterized in that: It also comprises a cell collection container (2), a first liquid storage container (3), a second liquid storage container (4), a third liquid storage container (5), a fourth liquid storage container (6), a fifth liquid storage container (7), a sixth liquid storage container (8), a seventh liquid storage container (9) and a controller, wherein the cell collection container (2), the first liquid storage container (3), the second liquid storage container (4), the third liquid storage container (5), the fourth liquid storage container (6), the fifth liquid storage container (7), the sixth liquid storage container (8) and the seventh liquid storage container (9) are all connected to the reactor (1), and the reactor (1) is electrically connected to the controller; The reactor (1) is used for cell culture and digestion; the cell collection container (2) is used for collecting cells cultured and digested in the reactor (1); the first liquid storage container (3) is used for storing cell suspension and providing cell suspension to the reactor (1); the second liquid storage container (4) is used for collecting liquid discharged from the reactor (1); the third liquid storage container (5) is used for storing pancreatic enzyme digestion liquid and providing pancreatic enzyme digestion liquid to the reactor (1); the fourth liquid storage container (6) is used for storing PBS buffer and providing PBS buffer to the reactor (1); the fifth liquid storage container (7) is used for storing stop solution and providing stop solution to the reactor (1). The sixth liquid storage container (8) is used to store nutrient solution and provide nutrient solution to the reactor (1); the seventh liquid storage container (9) is used to store waste liquid discharged from the reactor (1); the controller is used to control the first liquid storage container (3), the third liquid storage container (5), the fourth liquid storage container (6), the fifth liquid storage container (7) and the sixth liquid storage container (8) to automatically deliver corresponding cell suspension, trypsin digestion solution, PBS buffer, stop solution and nutrient solution to the reactor (1), and control the reactor (1) to automatically perform cell culture and digestion operations, and after the cell culture is completed, control the cell collection container (2) to automatically collect cells.

2. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 1, characterized in that: The cell collection container (2), the first liquid storage container (3) and the second liquid storage container (4) are all connected to the reactor (1) via a first pipeline (10); the third liquid storage container (5), the fourth liquid storage container (6) and the fifth liquid storage container (7) are all connected to the reactor (1) via a second pipeline (11); the sixth liquid storage container (8) is connected to the reactor (1) via a third pipeline (12); and the seventh liquid storage container (9) is connected to the reactor (1) via a fourth pipeline (13); The first pipeline (10) comprises a main pipeline A and three branch pipelines A, wherein the main pipeline A is connected to the reactor (1), and one end of each of the three branch pipelines A is connected to the main pipeline A, and the other end is connected to the cell collection container (2), the first liquid storage container (3), and the second liquid storage container (4). The second pipeline (11) comprises a main pipeline B and three branch pipelines B, wherein the main pipeline B is connected to the reactor (1), and one end of each of the three branch pipelines B is connected to the main pipeline B, and the other end is connected to the third liquid storage container (5), the fourth liquid storage container (6), and the fifth liquid storage container (7). Peristaltic pumps (14) are provided on the three branch pipelines A of the first pipeline (10), the three branch pipelines B of the second pipeline (11), the third pipeline (12) and the fourth pipeline (13), and the peristaltic pumps (14) are electrically connected to the controller.

3. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 1, characterized in that: The reactor (1) comprises a tank body (102) and a tank cover (101) arranged on the top of the tank body (102); a magnetic stirrer (103) is arranged on the top of the tank cover (101); a stirring paddle shaft (104) and a basket paddle base (105) are arranged inside the tank body (102); the upper end of the stirring paddle shaft (104) is connected to the output end of the magnetic stirrer (3), and the lower end is passed through the inside of the basket paddle base (105); the basket paddle base (105) is supported on the inner bottom wall of the tank body (102); and the magnetic stirrer (103) is electrically connected to a controller.

4. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 3, characterized in that: A DO electrode (106), a PH electrode (107) and a sampling tube (108) are also installed on the tank cover (101), and the control parts of the DO motor (106) and the PH electrode (107) are both located above the tank cover (101) and electrically connected to the controller. The detection parts are both extended inside the tank body (102) for contacting the liquid in the tank body (102). One end of the sampling tube (108) is extended above the tank cover (101), and the other end passes through the tank cover (101) and extends into the tank body (102) for contacting the liquid in the tank body (102).

5. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 3, characterized in that: A harvesting tube (109) is further provided inside the tank body (102), and the upper end of the harvesting tube (109) passes through the tank cover (101) and extends to above the tank cover (101), and is used to communicate with the cell collection container (2), the first liquid storage container (3) and the second liquid storage container (4); the lower end of the harvesting tube (109) passes through the basket paddle base (105) and is communicated with the inner cavity of the tank body at the lower part of the basket paddle base (105); and there is a gap of at least 5 cm between the harvesting tube (109) and the inner wall of the tank body (102).

6. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 3, characterized in that: The stirring paddle shaft (104) comprises a stirring paddle main shaft (104a), a stirring paddle connecting pipe (104b), a stirring paddle top cover (104c), a stirring paddle drainage chamber (104d), a stirring paddle shaft guide pipe (104e) and a stirring paddle shaft small blade (104g). The upper end of the stirring paddle main shaft (104a) is connected to the output end of the magnetic stirrer (103), and the lower end is connected to the stirring paddle connecting pipe (104b). The stirring paddle connecting pipe (104b) is connected to the stirring paddle top cover (104c). The paddle top cover (104c) is connected to the stirring paddle drainage chamber (104d), the stirring paddle drainage chamber (104d) is connected to the stirring paddle shaft guide tube (104f), the stirring paddle shaft guide tube (104f) passes through the basket paddle base (105) and extends to the inner cavity of the tank body at the lower part of the basket paddle base (105), and the stirring paddle shaft has a plurality of small blades (104g) which are annularly inclined and surround the outer peripheral wall of the stirring paddle shaft guide tube (104f) and are located inside the basket paddle base (105).

7. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 6, characterized in that: A plurality of stirring paddle discharge pipes (104e) are also provided around the stirring paddle discharge cavity (104d), and each stirring paddle discharge pipe (104e) is located on the upper part of the basket paddle base (105), and one end of each stirring paddle discharge pipe is connected to the stirring paddle discharge cavity (104d), and the other end is connected to the inner cavity of the tank body on the upper part of the basket paddle base (105).

8. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 6, characterized in that: The outer peripheral wall of the stirring paddle shaft flow guide tube (104f) is also surrounded by a stirring paddle shaft ventilation ring tube (104h); the interior of the stirring paddle shaft flow guide tube (104f) is also provided with a stirring paddle shaft central air pipe (104i); one end of the stirring paddle shaft ventilation ring tube (104h) is arranged on the outer wall of the stirring paddle shaft flow guide tube (104f) and is connected to the inner cavity of the basket paddle base (105), and the other end is connected to the stirring paddle shaft central air pipe (104i), and the stirring paddle shaft central air pipe (104i) is connected to the external atmosphere.

9. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 3, characterized in that: The basket paddle base (105) comprises a porous bottom plate (105a) and a liquid-conducting tube (105b). A plurality of base pillars (105c) are provided on the outer tube wall of the lower end of the liquid-conducting tube (105b). The porous bottom plate (105a) is sleeved on the outside of the liquid-conducting tube (105b) and fixed on the inner wall of the tank body (102). The base pillars (105c) are supported on the inner bottom wall of the tank body (102).

10. The fully automatic sheet-like carrier cell culture and digestion reaction device according to claim 3, characterized in that: A water jacket (110) is provided on the outside of the tank body (101), a support (111) is provided at the lower part of the water jacket (110), the water jacket (110) is fixedly supported on the support (111), a water inlet (112) and a water outlet (113) are provided on the support (111), and the water inlet (112) and the water outlet (113) are both communicated with the water jacket (109).

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