Liquid suction device of CTC enrichment dyeing equipment

Abstract

The utility model relates to the technical field of biological medicine, in particular to a liquid suction device of CTC enrichment dyeing equipment, which comprises a mounting frame, a moving component is arranged at the upper end of the mounting frame, a liquid suction component is mounted on the moving component, and the liquid suction component moves along with the moving component in the vertical direction and the horizontal direction. The liquid suction assembly comprises a limiting frame and a plurality of liquid suction devices installed in the limiting frame, and the lower ends of the liquid suction devices are connected with liquid suction heads in a sleeved mode. According to the utility model, the problem of low efficiency of manual reagent adding in the CTC cell enrichment dyeing process is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to a liquid aspiration device for CTC enrichment and staining equipment. Background Art

[0002] In recent years, emerging tumor diagnostic and detection technologies have emerged in the field of biomedical engineering, such as circulating tumor DNA (CTCs), a type of liquid biopsy. Research indicates that CTCs can be detected in peripheral blood before solid tumors develop, making CTC detection highly suitable for early screening and diagnosis of malignant tumors. CTC detection is also highly effective in prognosis, disease progression monitoring, recurrence prediction, monitoring of microscopic lesions after surgery, and designing and monitoring the efficacy of targeted drug therapy. It is currently a leading method for early screening and diagnosis of malignant tumors. This method avoids trauma to patients and facilitates sample acquisition. It can be widely used in immunological research, genetic research, and microbiological testing, and can even be applied to biochip and organoid research and development. However, the current mainstream method for detecting circulating tumor cells involves enrichment followed by detection. This process requires blotting, enrichment, and staining the collected body fluids, and each step requires multiple reagent additions, involving a wide range of reagent types. Currently, existing reagents on the market are manually taken and placed by staff. Due to the reduced efficiency of manual taking and placing, the efficiency of reagent identification is greatly reduced. Utility Model Content

[0003] In order to solve the above-mentioned problems of the prior art, the utility model provides a liquid aspiration device for a CTC enrichment and staining device, which solves the problem of low efficiency of manually adding reagents during the CTC cell enrichment and staining process.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a liquid aspiration device for a CTC enrichment and staining device, comprising a mounting frame, a movable component is provided at the upper end of the mounting frame, a liquid aspiration component is installed on the movable component, and the liquid aspiration component moves in the vertical and horizontal directions with the movable component, the liquid aspiration component comprises a limit frame and several liquid pipettes installed inside the limit frame, and a liquid aspiration head is connected to the lower end of the liquid pipette.

[0005] With the above structural design, the liquid-absorbing component is driven to move in the horizontal and vertical directions by the moving component, so that the liquid-absorbing component can automatically extract and transfer reagents, thereby improving the efficiency of reagent removal and placement, and solving the problem of tedious and laborious manual operation and low efficiency.

[0006] Preferably, the moving assembly includes a second lead screw and a slide rail arranged parallel to the upper end of the mounting frame, the second lead screw and the upper end of the slide rail are slidably connected to a beam, the middle position of the beam is fixedly connected to a first lead screw, the first lead screw is vertically arranged, and the limit frame is slidably connected to the first lead screw.

[0007] With the above structural design, the second lead screw drives the crossbeam to move, thereby driving the liquid suction component to move horizontally, and the second lead screw drives the liquid suction component to move vertically.

[0008] Preferably, a movable plate is provided at the lower end of the limit frame, and the movable plate is connected to the movable plate through a reset spring. The lower end of the pipette passes through the limit frame and the movable plate, and two limit blocks are fixedly connected to the relative positions of the inner side surface of the mounting frame, and the movable plate is located below the limit block.

[0009] With this structural design, a motor drives the pipette assembly along the second lead screw to move above the medical waste bin. At this point, the movable plate is positioned below the stop block, and the slider of the first lead screw moves upward. The stop block cooperates with the movable plate to remove the pipette tip. The pipette assembly must repeatedly add different reagents to the chip unit. After discarding a used pipette tip, the pipette can more quickly install the next one, saving time and eliminating the manual process of replacing pipette tips, increasing the device's automation level.

[0010] Preferably, the pipette includes a fixedly connected sleeve end and a connecting end from bottom to top, the connecting end passes through the limit frame and is fixedly connected to a fixing bolt, the bottom end of the sleeve end cooperates with the pipette head, and the pipette is provided with a vacuum pipe along its length direction, one end of the pipe opens at the sleeve end, and the other end of the pipe opens at the fixing bolt and is connected to the vacuum pump through a connecting pipe, and a compression spring is sleeved on the connecting end, and the two ends of the compression spring are respectively abutted against the sleeve end and the limit frame.

[0011] With the above structural design, the liquid-absorbing component moves downward along with the slider of the first screw, and the sleeve end gradually approaches the liquid-absorbing head placement rack below until the sleeve end is tightly fitted with the liquid-absorbing head. At this time, the compression spring plays a buffering role. After the liquid-absorbing head is clamped on the lower end of the pipette, the slider of the first screw moves upward, and the liquid-absorbing head moves upward with the pipette, realizing the automatic overlapping operation of the pipette with the liquid-absorbing head. The motor drives the liquid-absorbing component to move along the second screw to the top of the reagent box. The slider of the first screw drives the liquid-absorbing component to move downward into the reagent box. The negative pressure component is turned on to absorb the reagent. After the reagent is absorbed, the first screw drives the liquid-absorbing component to move upward and reset. The motor drives the liquid-absorbing component to move along the second screw to the top of the chip unit for liquid addition. After each liquid addition, the liquid-absorbing component moves upward and resets.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. The mobile component drives the liquid suction component to move in the horizontal and vertical directions, so that the liquid suction component can automatically extract and transfer reagents, improving the efficiency of reagent placement and solving the problem of tedious and laborious manual operation and low efficiency.

[0014] 2. The pipette assembly needs to repeatedly add different reagents into the chip unit. After the pipette head is discarded, the next one can be installed more quickly, saving time and eliminating the process of manually replacing the pipette head, thereby increasing the degree of automation of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural diagram of the utility model;

[0016] Figure 2 This is a schematic structural diagram of the liquid-absorbing component of the utility model;

[0017] Figure 3 It is a structural schematic diagram of the liquid aspirator of the present utility model. DETAILED DESCRIPTION

[0018] See also Figure 1-3 The present invention provides a technical solution: a liquid aspiration device for a CTC enrichment and staining device, comprising a mounting frame 1, a movable assembly 2 disposed at the upper end of the mounting frame 1, a liquid aspiration assembly 6 mounted on the movable assembly 2, the liquid aspiration assembly 6 moving vertically and horizontally with the movable assembly 2, the liquid aspiration assembly 6 comprising a limiting frame 601 and a plurality of liquid aspirators 5 mounted within the limiting frame 601, the lower end of each liquid aspirator 5 being sleeved with a liquid aspiration head 9. The liquid aspirator 6 is driven by the movable assembly 2 to move horizontally and vertically, enabling the liquid aspiration assembly 6 to automatically extract and transfer reagents, thereby improving the efficiency of reagent placement and retrieval and resolving the problem of cumbersome, laborious, and inefficient manual operation.

[0019] The moving assembly 2 includes a second lead screw 201 and a slide rail 202 arranged parallel to the upper end of the mounting frame 1. The upper ends of the second lead screw 201 and the slide rail 202 are slidably connected to a crossbeam 3. A first lead screw 4 is fixedly connected to the middle position of the crossbeam 3. The first lead screw 4 is vertically arranged, and the limit frame 601 is slidably connected to the first lead screw 4. The second lead screw 201 drives the crossbeam 3 to move, thereby driving the liquid suction assembly 6 to move horizontally. The first lead screw 4 drives the liquid suction assembly 6 to move vertically.

[0020] The lower end of the limit frame 601 is provided with a movable plate 603, and the movable plate 603 is connected to the movable plate 603 by a reset spring 602. The lower end of the pipette 5 passes through the limit frame 601 and the movable plate 603. The relative positions of the inner side surfaces of the mounting frame 1 are fixedly connected with two limit blocks 7, and the movable plate 603 is located below the limit blocks 7. The motor drives the pipette assembly 6 to move along the second screw 201 to the top of the medical waste box. At this time, the movable plate 603 is located below the limit block 7, and the slider of the first screw 4 moves upward. With the cooperation of the limit block 7, the movable plate 603 removes the pipette head 9. The pipette assembly 6 needs to repeatedly add different reagents to the chip unit. After the pipette 5 discards the used pipette head 9, it can install the next pipette head 9 more quickly, saving time, eliminating the process of manually replacing the pipette head 9, and increasing the degree of automation of the device.

[0021] The pipette 5 includes a fixedly connected sleeve end 501 and a connecting end 502 from bottom to top, the connecting end 502 passes through the limit frame 601 and is fixedly connected to a fixing bolt 503, the bottom end of the sleeve end 501 cooperates with the pipette head 9, and the pipette 5 is provided with a vacuum pipe along its length direction, one end of the pipe opens at the sleeve end 501, and the other end of the pipe opens at the fixing bolt 503 and is connected to the vacuum pump through the connecting pipe 8, a compression spring 504 is sleeved on the connecting end 502, and the two ends of the compression spring 504 are respectively in contact with the sleeve end 501 and the limit frame 601. As the slider of the first lead screw 4 moves downward, the liquid pipetting assembly 6 gradually approaches the liquid pipetting head 9 placement rack below until the sleeve end 501 is tightly fitted with the liquid pipetting head 9. At this time, the compression spring 504 plays a buffering role. After the liquid pipetting head 9 is clamped on the lower end of the pipette 5, the slider of the first lead screw 4 moves upward, and the liquid pipetting head 9 moves upward with the pipette 5, so that the pipette 5 automatically overlaps the liquid pipetting head 9. The motor drives the liquid pipetting assembly 6 to move along the second lead screw 201 to the top of the reagent box. The slider of the first lead screw 4 drives the liquid pipetting assembly 6 to move downward into the reagent box. The negative pressure assembly is turned on to absorb the reagent. After the reagent is absorbed, the first lead screw 4 drives the liquid pipetting assembly 6 to move upward and reset. The motor drives the liquid pipetting assembly 6 to move along the second lead screw 201 to the top of the chip unit for liquid addition. After each liquid addition, the liquid pipetting assembly 6 moves upward and resets.

[0022] Working principle: before each time the liquid-absorbing component 6 absorbs the reagent, the liquid-absorbing head 9 must first be installed. After one reagent is injected, the liquid-absorbing head 9 needs to be removed. As the slider of the first screw 4 moves downward, the sleeve end 501 gradually approaches the liquid-absorbing head 9 placement rack below until the sleeve end 501 is tightly fitted with the liquid-absorbing head 9. At this time, the compression spring 504 plays a buffering role. After the liquid-absorbing head 9 is clamped on the lower end of the pipette 5, the slider of the first screw 4 moves upward, and the liquid-absorbing head 9 moves upward with the pipette 5, so that the pipette 5 automatically overlaps the liquid-absorbing head 9 operation. The motor drives the liquid-absorbing component 6 to move along the second screw 201 to the top of the reagent box. The slider of the first screw 4 drives the liquid-absorbing component 6 to move downward into the reagent box. The vacuum pump is turned on to absorb the reagent. After the reagent is absorbed, the first screw 4 drives the liquid-absorbing component 6 to move upward and reset. The motor drives the liquid-absorbing component 6 to move along the second screw 201 to the top of the chip unit for liquid addition. After each liquid addition, the liquid-absorbing component 6 moves upward and reset. The motor drives the pipetting assembly 6 to move along the second lead screw 201 to the top of the medical waste bin. At this point, the movable plate 603 is located below the stop block 7. The slider of the first lead screw 4 moves upward, and with the cooperation of the stop block 7, the movable plate 603 removes the pipetting head 9. The pipetting assembly 6 needs to repeatedly add different reagents to the chip unit. After the pipette 5 discards the used pipetting head 9, it can more quickly install the next pipetting head 9.

[0023] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in this specification for the understanding and reading of those familiar with this technology. They are not used to limit the conditions for the implementation of this utility model and therefore have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed in this utility model without affecting the efficacy and purpose of the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description and are not used to limit the scope of the implementation of this utility model. Changes or adjustments in their relative relationships should also be considered as the scope of the implementation of this utility model without substantially changing the technical content.

[0024] The present invention has been described above with reference to preferred embodiments. However, the scope of protection of the present invention is not limited thereto. All technical solutions falling within the scope of the claims are within the scope of protection of the present invention. Various improvements may be made to the present invention, and components may be replaced with equivalents without departing from the scope of the present invention. In particular, the various technical features described in the various embodiments may be combined in any manner, as long as there are no structural conflicts.

Claims

1. A liquid aspiration device for a CTC enrichment and staining apparatus, comprising a mounting frame (1), characterized in that: A moving assembly (2) is provided at the upper end of the mounting frame (1), a liquid suction assembly (6) is installed on the moving assembly (2), and the liquid suction assembly (6) moves in the vertical and horizontal directions along with the moving assembly (2), the liquid suction assembly (6) comprises a limit frame (601) and a plurality of liquid suction devices (5) installed inside the limit frame (601), and a liquid suction head (9) is sleeved on the lower end of the liquid suction device (5).

2. The liquid aspiration device of the CTC enrichment and staining apparatus according to claim 1, characterized in that: The moving assembly (2) comprises a second lead screw (201) and a slide rail (202) arranged in parallel at the upper end of the mounting frame (1); the upper ends of the second lead screw (201) and the slide rail (202) are slidably connected to a crossbeam (3); a first lead screw (4) is fixedly connected to the middle position of the crossbeam (3); the first lead screw (4) is arranged vertically, and the limit frame (601) is slidably connected to the first lead screw (4).

3. The liquid aspiration device of the CTC enrichment and staining apparatus according to claim 1, characterized in that: A movable plate (603) is provided at the lower end of the limit frame (601), and the movable plate (603) is connected to the movable plate (603) via a reset spring (602). The lower end of the pipette (5) passes through the limit frame (601) and the movable plate (603). Two limit blocks (7) are fixedly connected to the relative positions of the inner side surface of the mounting frame (1), and the movable plate (603) is located below the limit block (7).

4. The liquid aspiration device of the CTC enrichment and staining apparatus according to claim 1, characterized in that: The aspirator (5) includes, from bottom to top, a fixedly connected sleeve end (501) and a connecting end (502), the connecting end (502) passes through the limit frame (601) and is fixedly connected to a fixing bolt (503), the bottom end of the sleeve end (501) cooperates with the aspirator head (9), the aspirator (5) is provided with a vacuum pipe along its length direction, one end of the pipe opens at the sleeve end (501), the other end of the pipe opens at the fixing bolt (503) and is connected to the vacuum pump through a connecting pipe (8), a compression spring (504) is sleeved on the connecting end (502), and the two ends of the compression spring (504) are respectively in contact with the sleeve end (501) and the limit frame (601).

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