Scratching device for cell scratch experiment

Abstract

The utility model relates to the technical field of cell culture, in particular to a scratching device for a cell scratching experiment, which comprises a supporting sheet, a first fixing seat, a second fixing seat and at least one elastic sheet bent in an arc shape, a first arc-shaped groove used for being placed on one side of the top of a culture hole is formed in the bottom of the first fixing seat, and a second arc-shaped groove is formed in the bottom of the second fixing seat. A second arc-shaped groove used for being placed on the other side of the top of the culture hole is formed in the bottom of the second fixing base, one end of each elastic piece is fixed to the connecting position of the bottom of the supporting piece and the inner side of the first fixing base, at least one inserting hole is formed in the inner side, corresponding to the second fixing base, of the supporting piece, and the other end of each elastic piece penetrates through the corresponding inserting hole in a close fit mode and extends out. The scratching device can be placed on the side wall of the culture hole through the supporting piece and the fixing bases on the two sides, the elastic piece can press out a needed scratch in the culture hole after being placed, the wall attaching length of the bent portion of the elastic piece can be adjusted according to the size of the culture hole, and the scratching device is simple in structure and convenient to use.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a scratching device for cell scratching experiments. Background Art

[0002] Cell migration is a directional movement through cell deformation. The cell scratch assay simulates the process of cell migration in vivo to a certain extent and is the simplest and most commonly used in vitro test method for studying cell migration. Traditional cell scratch assays are mainly performed manually, such as pressing a micropipette tip (commonly specified as 10μL) with a finger to cause the tip to slide across the cell culture dish at a certain speed, at which point the cells are in an attached state. However, due to the uncertainty of manual operation, this method can easily result in irregular scratch shapes and unclear edges. In addition, due to individual differences in the force-applying subject, it is difficult to maintain consistent applied force, which greatly complicates the analysis and statistics of subsequent experimental results.

[0003] Chinese utility model patent publication number CN220867368U discloses a scratching device specifically for six-well plates used in cell scratch experiments. The device comprises a base with a circular opening in the center that matches the circular hole of the six-well plate. Two opposite chute slots are provided on either side of the base. A scratcher is located above the base. A slider is provided at the bottom of the scratcher, which inserts into the chute and slidably connects to the base. The scratcher includes a carriage with a chute slot along its length. Rectangular windows are provided at each end of the carriage. A latch is provided vertically within the rectangular window. The latch is slidably connected to the slider, and a return spring is provided on the latch below the slider. A connecting hole is provided on the inside of the slider, through which a sliding beam is inserted into the slider. The sliding beam is slidably connected to a lance, and a sliding needle is provided at the bottom of the lance. By sliding the scratcher and carriage, the lance can slide anywhere in the circular hole of the six-well plate, while the lance can only move back and forth along the carriage. This prevents skew and distortion of the scratch, ensures the quality and consistency of the scratch, and improves experimental reproducibility. However, this patent also suffers from the drawbacks of a complex structure and inconvenience. Summary of the Invention

[0004] In order to overcome the shortcomings and deficiencies in the prior art, the purpose of the present invention is to provide a scratching device for cell scratch experiments.

[0005] The purpose of the utility model is achieved through the following technical solutions: A scratch device for cell scratch experiments, comprising a support plate for being placed horizontally on one of the culture wells of a multi-well plate, a first fixing seat fixed to one side of the bottom of the support plate, a second fixing seat fixed to the other side of the bottom of the support plate, and at least one arc-shaped spring piece, the bottom of the first fixing seat is provided with a first arc-shaped groove for being placed on one side of the top of the culture well, the bottom of the second fixing seat is provided with a second arc-shaped groove for being placed on the other side of the top of the culture well, one end of each spring piece is fixed to the connection between the bottom of the support plate and the inner side of the first fixing seat, the inner side of the support plate corresponding to the second fixing seat is provided with at least one socket, and the other end of each spring piece is tightly fitted through the corresponding socket and extends out.

[0006] Furthermore, the width of the support sheet is 0.5-2.5 cm.

[0007] Furthermore, the width of the spring is 0.6-1.0 mm.

[0008] Furthermore, the number of the spring pieces is two, the two spring pieces are arranged in parallel and spaced apart, and the distance between the two spring pieces is 0.5-1.0 cm.

[0009] Furthermore, the number of the spring pieces is three to five, and the three to five spring pieces are arranged in parallel and spaced apart in sequence, and the distance between two adjacent spring pieces is 0.5-1.0 cm.

[0010] The beneficial effect of the present invention is that the scratching device of the present invention can be placed on the side wall of the culture well by adopting a support plate and fixed seats on both sides. After placement, the spring piece will press out the required scratches in the culture well, and the length of the curved part of the spring piece attached to the wall can also be adjusted according to the size of the culture well. The structure is simple and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a three-dimensional diagram of the present utility model.

[0012] Figure 2 This is a usage state diagram of the utility model.

[0013] Figure 3 It is a bottom view of the utility model when the spring piece is one.

[0014] Figure 4 It is a bottom view of the utility model when there are three spring pieces.

[0015] Figure 5 It is a bottom view of the utility model when there are five spring pieces.

[0016] The reference numerals are: culture hole 10 , support plate 1 , insertion hole 11 , first fixing seat 2 , first arc-shaped groove 21 , second fixing seat 3 , second arc-shaped groove 31 , and spring piece 4 . DETAILED DESCRIPTION

[0017] In order to facilitate the understanding of those skilled in the art, the following embodiments and accompanying drawings are provided. Figure 1-5 To further illustrate the present invention, the contents mentioned in the implementation manner are not intended to limit the present invention.

[0018] See Figure 1-5 A scratch device for a cell scratch experiment comprises a support sheet 1 for being placed horizontally on one of the culture wells 10 of a multi-well plate, a first fixing seat 2 fixed to one side of the bottom of the support sheet 1, a second fixing seat 3 fixed to the other side of the bottom of the support sheet 1, and at least one arc-shaped spring piece 4. The bottom of the first fixing seat 2 is provided with a first arc-shaped groove 21 for being placed on one side of the top of the culture well 10, and the bottom of the second fixing seat 3 is provided with a second arc-shaped groove 31 for being placed on the other side of the top of the culture well 10. One end of each spring piece 4 is fixed to the connection between the bottom of the support sheet 1 and the inner side of the first fixing seat 2. The inner side of the support sheet 1 corresponding to the second fixing seat 3 is provided with at least one socket 11. The other end of each spring piece 4 is tightly fitted through the corresponding socket 11 and extends out.

[0019] The scratching device of the present invention adopts a support plate 1 and fixed seats on both sides, which can be placed on the side wall of the culture hole 10. After placement, the spring piece 4 will press out the required scratches in the culture hole 10, and the length of the curved part of the spring piece 4 attached to the wall can also be adjusted according to the size of the culture hole 10. The structure is simple and easy to use.

[0020] In this embodiment, the width of the support sheet 1 is 0.5-2.5 cm. The width of the support sheet 1 can be adjusted as needed. When only one scratch is required, the width of the support sheet 1 can be 0.5 cm or 1 cm. When multiple scratches need to be pressed out in sequence, the width of the support sheet 1 can be 1.5 cm, 2.0 cm, or 2.5 cm, etc.

[0021] In this embodiment, the width of the spring piece 4 is 0.6-1.0 mm. The width of the spring piece 4 can be set to 0.6 cm, 0.8 cm or 1.0 cm as needed.

[0022] In this embodiment, there are two spring pieces 4, which are arranged in parallel and spaced apart, with a distance of 0.5-1.0 cm between the two spring pieces 4. The arrangement of the two spring pieces 4 can produce two scratches at one time as needed.

[0023] In this embodiment, the number of the spring pieces 4 is three to five, and the three to five spring pieces 4 are arranged in parallel and spaced apart in sequence, with the distance between two adjacent spring pieces 4 being 0.5-1.0 cm. The arrangement of the three to five spring pieces 4 can press out three to five scratches at a time as needed, wherein the bottom view when there are three spring pieces 4 is as follows: Figure 4As shown, the bottom view when the spring pieces 4 are five is as follows Figure 5 shown.

[0024] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the scope of protection of the present invention.

Claims

1. A scratch device for cell scratch experiment, characterized by: It includes a support plate for being placed horizontally on one of the culture holes of a multi-well plate, a first fixing seat fixed to one side of the bottom of the support plate, a second fixing seat fixed to the other side of the bottom of the support plate, and at least one arc-shaped spring piece. The bottom of the first fixing seat is provided with a first arc-shaped groove for being placed on one side of the top of the culture hole, and the bottom of the second fixing seat is provided with a second arc-shaped groove for being placed on the other side of the top of the culture hole. One end of each spring piece is fixed to the connection between the bottom of the support plate and the inner side of the first fixing seat. The inner side of the support plate corresponding to the second fixing seat is provided with at least one socket. The other end of each spring piece is tightly fitted through the corresponding socket and extends out.

2. A scratch device for cell scratch experiment according to claim 1, characterized in that: The width of the support sheet is 0.5-2.5 cm.

3. A scratch device for cell scratch experiment according to claim 1, characterized in that: The width of the spring piece is 0.6-1.0 mm.

4. A scratch device for cell scratch experiment according to claim 1, characterized in that: There are two spring pieces, which are arranged in parallel and spaced apart, and the distance between the two spring pieces is 0.5-1.0 cm.

5. The scratch device for cell scratch experiment according to claim 1, characterized in that: The number of the spring pieces is three to five, and the three to five spring pieces are arranged in parallel and spaced apart in sequence, and the distance between two adjacent spring pieces is 0.5-1.0 cm.

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