Highly integrated nucleic acid extraction and purification mechanical arm and nucleic acid POCT detector

Abstract

The utility model discloses a highly integrated nucleic acid extraction and purification mechanical arm and a nucleic acid POCT detector, and relates to the technical field of nucleic acid detection instruments. The nucleic acid extraction and purification mechanical arm comprises a moving module, and a suction nozzle, a clamping jaw and a magnetic bar which are arranged on the moving module; the suction nozzle is used for sucking a to-be-tested sample; the clamping jaw is used for clamping a sample tube and / or a reaction tube and carrying out cover opening / closing treatment on the sample tube and / or the reaction tube; the magnetic bar is used for extracting and purifying nucleic acid of a to-be-tested sample; the moving module is used for driving the suction nozzle, the clamping jaw and the magnetic bar to move in at least one direction. The utility model provides a mechanical arm for extracting and purifying nucleic acid. The efficiency of extracting and purifying nucleic acid is improved. The nucleic acid POCT detector can automatically complete nucleic acid detection of 1-21 pathogens in the whole process within 30 minutes and can send a result. Compared with a traditional fluorescent PCR method, the method has the advantages that the detection time is greatly shortened, the automation degree and the convenience are greatly improved, the instrument size is greatly reduced, the requirements on professionals and places are low, and the application range is very wide.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection instruments, and in particular to a highly integrated nucleic acid extraction and purification mechanical arm and a nucleic acid POCT detector. Background Art

[0002] The existing nucleic acid detection instrument is equipped with an X-axis module, and a suction nozzle and a magnetic rod are fixed on the X-axis module. The suction nozzle sucks the sample from the sample tube, then drops it into the reagent tank, and then uses the magnetic rod to extract and purify it and transfer it to the reaction tube. Finally, a PCR amplification detector is used for nucleic acid detection.

[0003] However, during the testing process, various consumables such as sample tubes and reaction tubes need to be manually taken in and out and the caps of the sample tubes and reaction tubes need to be opened / closed, which greatly affects the cycle of nucleic acid testing. Utility Model Content

[0004] The main purpose of the utility model is to provide a highly integrated nucleic acid extraction and purification robotic arm and a nucleic acid POCT detector, aiming to improve the efficiency of nucleic acid extraction and purification and shorten the nucleic acid detection cycle.

[0005] To achieve the above objectives, the present invention proposes a highly integrated nucleic acid extraction and purification robotic arm, comprising:

[0006] Mobile modules;

[0007] A suction nozzle, detachably provided on the mobile module, and used for sucking the sample to be tested;

[0008] a clamping jaw, detachably provided on the movable module, for clamping a sample tube and / or a reaction tube and performing a cap opening / closing process on the sample tube and / or the reaction tube; and

[0009] A magnetic bar, detachably mounted on the mobile module, for extracting and purifying nucleic acid from a sample to be tested;

[0010] The moving module is used to drive the suction nozzle, the clamping claw and the magnetic bar to move in at least one direction.

[0011] Optionally, the movable module includes a first driving member, a slide rail and a slider, the slider is slidably arranged on the slide rail and is driven and connected to the first driving member, the first driving member is used to drive the slider to slide along the length direction of the slide rail, and the clamp, the suction nozzle and the magnetic bar are respectively movably arranged on the slider.

[0012] Optionally, the moving module further includes a second driving member, which is disposed on the slider and is drivingly connected to the clamping jaw, and is used to drive the clamping jaw to open and close.

[0013] Optionally, the movable module further includes a third driving member, which is disposed on the slider and is drivingly connected to the clamping claw, and is used to drive the clamping claw to rotate to unscrew the tube cover.

[0014] Optionally, the moving module further includes a fourth driving member, which is disposed on the slider and is drivingly connected to the clamping claw, and is used to drive the clamping claw to perform lifting motion.

[0015] Optionally, the movable module further includes a fifth driving member, which is disposed on the slider and is drivingly connected to the suction nozzle, and is used to drive the suction nozzle to perform lifting motion.

[0016] Optionally, the moving module further includes a sixth driving member, which is disposed on the slider and is drivingly connected to the magnetic bar, and is used to drive the magnetic bar to perform lifting motion.

[0017] Optionally, the output end of the sixth driving member is connected to the magnetic rod via a lifting block, and a buffer member is provided between the lifting block and the fixing seat of the sixth driving member.

[0018] Optionally, the clamp includes a mounting seat, an insertion rod and two clamping blocks, one end of the insertion rod is connected to the second driving member, the other end of the insertion rod is movably inserted in the mounting seat and is provided with two inclined surfaces, the two clamping blocks are arranged opposite to each other on the mounting seat and both are against the inclined surfaces on the insertion rod, and the mounting seat is connected to the third driving member through a belt transmission mechanism.

[0019] To achieve the above-mentioned object, the present invention further proposes a nucleic acid POCT detector, comprising the nucleic acid extraction and purification robotic arm as described above, wherein the nucleic acid extraction and purification robotic arm comprises:

[0020] Mobile modules;

[0021] A suction nozzle, detachably provided on the mobile module, and used for sucking the sample to be tested;

[0022] a clamping jaw, detachably provided on the movable module, for clamping a sample tube and / or a reaction tube and performing a cap opening / closing process on the sample tube and / or the reaction tube; and

[0023] A magnetic bar, detachably mounted on the mobile module, for extracting and purifying nucleic acid from a sample to be tested;

[0024] The moving module is used to drive the suction nozzle, the clamping claw and the magnetic bar to move in at least one direction.

[0025] In the technical solution of the present invention, the nucleic acid extraction and purification robot includes a mobile module and a suction nozzle, a clamp and a magnetic rod provided thereon; the suction nozzle is used to suck the sample to be tested; the clamp is used to clamp the sample tube and / or reaction tube and open / close the cover of the sample tube and / or reaction tube; the magnetic rod is used to extract and purify the nucleic acid of the sample to be tested; the mobile module is used to drive the suction nozzle, the clamp and the magnetic rod to move in at least one direction. It can be understood that the present invention can take and place sample tubes and reaction tubes by arranging clamps on the mobile module, and can automatically open / close the tube covers of the sample tubes and reaction tubes, eliminating the need for manual taking and placing of sample tubes and reaction tubes and other consumables, and eliminating the need for manual opening / closing of the tube covers of the sample tubes and reaction tubes, thereby greatly improving the efficiency of nucleic acid extraction and purification, thereby significantly shortening the nucleic acid detection cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0027] Figure 1 This is a structural diagram of an embodiment of the nucleic acid extraction and purification robotic arm of the present invention;

[0028] Figure 2 This is a schematic structural diagram of an embodiment of the nucleic acid POCT detector of the present utility model.

[0029] Description of Figure Numbers:

[0030] 10. Moving module; 20. Suction nozzle; 30. Clamp; 40. Magnetic rod; 101. Sample tube; 102. Reaction tube; 103. Reagent tank; 11. First driving member; 12. Slide rail; 13. Slider; 14. Second driving member; 15. Third driving member; 16. Fourth driving member; 17. Fifth driving member; 18. Sixth driving member; 19. Buffer; 31. Mounting seat; 32. Clamp.

[0031] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0034] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0035] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, if the meaning of "and / or" appearing in the full text is to include three parallel schemes, taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme in which A and B are satisfied at the same time. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0036] The present invention provides a highly integrated nucleic acid extraction and purification robotic arm, which can be applicable to nucleic acid POCT detectors, but is not limited here.

[0037] Reference Figure 1 and Figure 2In one embodiment of the present invention, the nucleic acid extraction and purification robot arm includes a mobile module 10 and a suction nozzle 20, a clamping jaw 30 and a magnetic rod 40 detachably arranged thereon; the suction nozzle 20 is used to suck the sample to be tested; the clamping jaw 30 is used to clamp the sample tube 101 and / or the reaction tube 102 and open / close the cover of the sample tube 101 and / or the reaction tube 102; the magnetic rod 40 is detachably arranged on the mobile module 10, and the magnetic rod 40 is used to extract and purify the nucleic acid of the sample to be tested; the mobile module 10 is used to drive the suction nozzle 20, the clamping jaw 30 and the magnetic rod 40 to move in at least one direction.

[0038] In this embodiment, the movable module 10 may be an X-axis module, a Y-axis module, or a Z-axis module, or may be an XYZ three-axis module, so as to improve the efficiency of nucleic acid extraction and purification.

[0039] It can be understood that the utility model provides a clamping claw 30 on the mobile module 10 to take and place the sample tube 101 and the reaction tube 102, and can automatically open / close the tube covers of the sample tube 101 and the reaction tube 102. There is no need to manually take and place various consumables such as the sample tube 101 and the reaction tube 102, and there is no need to manually open / close the tube covers of the sample tube 101 and the reaction tube 102, which greatly improves the efficiency of nucleic acid extraction and purification, thereby significantly shortening the nucleic acid detection cycle.

[0040] This utility model improves the nucleic acid extraction and purification robotic arm of a nucleic acid point-of-care (POCT) detector, significantly increasing the efficiency of nucleic acid extraction and purification. The entire testing process can be controlled within 30 minutes, fully automatically completing nucleic acid testing for 1 to 21 pathogens and automatically transmitting the results. Compared to traditional fluorescent PCR methods, this method significantly shortens testing time and improves convenience, reduces the requirements for professional expertise and facilities, and has a wide range of applications.

[0041] In order to realize that the nozzle 20, the clamping claw 30 and the magnetic rod 40 can move along the length direction or the width direction of the nucleic acid POCT detector, refer to Figure 1 and Figure 2 In one embodiment, the movable module 10 includes a first driving member 11, a slide rail 12 and a slider 13. The slider 13 is slidably arranged on the slide rail 12 and is driven and connected to the first driving member 11. The first driving member 11 is used to drive the slider 13 to slide along the length direction of the slide rail 12. The clamping jaw 30, the suction nozzle 20 and the magnetic rod 40 are respectively movably arranged on the slider 13.

[0042] In this embodiment, the movable module 10 further includes a second driving member 14 and a third driving member 15. The second driving member 14 is disposed on the slider 13 and is drivingly connected to the clamping jaws 30. The second driving member 14 is used to drive the clamping jaws 30 to open and close, thereby adjusting the size of the clamping opening and tightening or loosening an object. The third driving member 15 is disposed on the slider 13 and is drivingly connected to the clamping jaws 30. The third driving member 15 is used to rotate the clamping jaws 30 to loosen or tighten the pipe cap.

[0043] The clamping jaw 30 includes a mounting base 31, an insertion rod, and two clamping blocks 32. One end of the insertion rod is connected to the second drive member 14, while the other end of the insertion rod is movably inserted into the mounting base 31 and has two inclined surfaces. The two clamping blocks 32 are positioned opposite each other on the mounting base 31 and abut against the inclined surfaces of the insertion rod. The mounting base 31 is connected to the third drive member 15 via a belt drive mechanism. This allows the clamping jaw 30 to open and close to accommodate different sizes of liquid tubes or bottles and meet the requirements of automatic lid opening and closing, thus providing a wider range of applications.

[0044] In this embodiment, in order to facilitate the taking and placing of items such as the sample tube 101 and the reaction tube 102, the movable module 10 may further include a fourth driving member 16. The fourth driving member 16 is arranged on the slider 13 and is connected to the clamping jaw 30. The fourth driving member 16 is used to drive the clamping jaw 30 to perform lifting and lowering movements.

[0045] Similarly, the mobile module 10 may also include a fifth drive member 17 and a sixth drive member 18. The fifth drive member 17 is disposed on the slider 13 and is drivably connected to the nozzle 20. The fifth drive member 17 is used to drive the nozzle 20 to move up and down to facilitate the suction and dripping of liquids. The sixth drive member 18 is disposed on the slider 13 and is drivably connected to the magnetic rod 40. The sixth drive member 18 is used to drive the magnetic rod 40 to move up and down to enhance the convenience of nucleic acid extraction and purification.

[0046] The first driving member 11, the second driving member 14, the third driving member 15, the fourth driving member 16, the fifth driving member 17 and the sixth driving member 18 can be motors, oil cylinders, air cylinders, etc., and can be the same or different, which is not limited here.

[0047] In addition, in order to reduce the damage caused by collision between the moving parts of the drive mechanism and other parts and to increase the service life, refer to Figure 1 and Figure 2 In some embodiments, the output end of the sixth driving member 18 can be connected to the magnetic rod 40 via a lifting block, and a buffer member 19 such as a spring is provided between the lifting block and the fixing base of the sixth driving member 18. Similarly, buffer members 19 can also be provided on the fixing bases of the other aforementioned driving members, and this is not limited here.

[0048] The present invention also proposes a nucleic acid POCT detector, which includes a nucleic acid extraction and purification robotic arm. The specific structure of the nucleic acid extraction and purification robotic arm refers to the above embodiment. Since the nucleic acid POCT detector proposed by the present invention includes all schemes of all embodiments of the above nucleic acid extraction and purification robotic arm, it has at least the same technical effects as the nucleic acid extraction and purification robotic arm, which will not be elaborated here one by one.

[0049] The above description is merely an optional embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by utilizing the contents of the present invention specification and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A highly integrated nucleic acid extraction and purification robotic arm, characterized in that: The nucleic acid extraction and purification robotic arm comprises: Mobile modules; A suction nozzle, detachably provided on the mobile module, and used for sucking the sample to be tested; a clamping jaw, detachably provided on the movable module, for clamping a sample tube and / or a reaction tube and performing a cap opening / closing process on the sample tube and / or the reaction tube; and A magnetic bar, detachably mounted on the mobile module, for extracting and purifying nucleic acid from a sample to be tested; The moving module is used to drive the suction nozzle, the clamping claw and the magnetic bar to move in at least one direction.

2. The nucleic acid extraction and purification robotic arm according to claim 1, wherein: The movable module includes a first driving member, a slide rail and a slider. The slider is slidably arranged on the slide rail and is driven and connected to the first driving member. The first driving member is used to drive the slider to slide along the length direction of the slide rail. The clamping claw, the suction nozzle and the magnetic bar are respectively movably arranged on the slider.

3. The nucleic acid extraction and purification robotic arm according to claim 2, characterized in that: The movable module further includes a second driving member, which is disposed on the slider and is drivingly connected to the clamping jaws, and is used to drive the clamping jaws to open and close.

4. The nucleic acid extraction and purification robotic arm according to claim 3, wherein: The moving module further includes a third driving member, which is disposed on the slider and is drivingly connected to the clamping claw. The third driving member is used to drive the clamping claw to rotate so as to unscrew the tube cover.

5. The nucleic acid extraction and purification robotic arm according to claim 4, characterized in that: The moving module further includes a fourth driving member, which is disposed on the slider and is drivingly connected to the clamping claw. The fourth driving member is used to drive the clamping claw to perform lifting motion.

6. The nucleic acid extraction and purification robotic arm according to claim 2, characterized in that: The moving module further includes a fifth driving member, which is disposed on the slider and is drivingly connected to the suction nozzle. The fifth driving member is used to drive the suction nozzle to perform lifting motion.

7. The nucleic acid extraction and purification robotic arm according to claim 2, characterized in that: The moving module further includes a sixth driving member, which is disposed on the slider and is drivingly connected to the magnetic bar. The sixth driving member is used to drive the magnetic bar to move up and down.

8. The nucleic acid extraction and purification robotic arm according to claim 7, characterized in that: The output end of the sixth driving member is connected to the magnetic rod through a lifting block, and a buffer member is provided between the lifting block and the fixing seat of the sixth driving member.

9. The nucleic acid extraction and purification robotic arm according to claim 4, characterized in that: The clamping claw includes a mounting seat, an insertion rod and two clamping blocks. One end of the insertion rod is connected to the second driving member, and the other end of the insertion rod is movably inserted in the mounting seat and is provided with two inclined surfaces. The two clamping blocks are arranged on the mounting seat opposite to each other and both are against the inclined surfaces on the insertion rod. The mounting seat is connected to the third driving member through a belt transmission mechanism.

10. A nucleic acid POCT detector, characterized in that: It comprises the nucleic acid extraction and purification robotic arm according to any one of claims 1 to 9.

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