Low-temperature tissue grinding device
By setting up an ice water storage chamber in the tissue grinding device, the problem of RNA and protein degradation caused by grinding heat is solved, low-temperature grinding is achieved, and the accuracy of experimental results is ensured.
Patent Information
- Application Number
- CN202422528803.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-19
- Publication Date
- 2025-09-16
- Estimated Expiration
- 2034-10-19
AI Technical Summary
The heat generated by existing tissue grinding devices during the grinding process causes RNA and protein degradation, affecting the accuracy of experimental results.
A low-temperature tissue grinding device was designed, which includes a mortar and a grinding rod. An ice water storage chamber is provided outside the mortar. By adding ice water, a low-temperature environment is maintained to eliminate grinding heat and ensure that the sample is ground at a low temperature.
Effectively keep tissue samples ground in a low-temperature environment, reduce RNA and protein degradation, and ensure the accuracy of subsequent experiments.
Smart Images
Figure CN223346560U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of grinding devices, and in particular to a low-temperature tissue grinding device. Background Art
[0002] Before conducting various biotechnology experiments (such as western blot experiments or PCR (polymerase chain reaction) experiments), animal and plant experimental sample tissues need to be processed, and the tissues need to be ground and added with lysis buffer to extract proteins or RNA.
[0003] The tissues or cells of animals and plants are sensitive to temperature. A lot of heat will be generated during the grinding process. Under high temperature, the RNA or protein in the animal and plant cells will be rapidly degraded, resulting in poor quality of the extracted protein or RNA, which will affect subsequent experimental results. Utility Model Content
[0004] The purpose of the present application is to provide a low-temperature tissue grinding device to solve the problem that existing tissue grinding devices generate heat when grinding tissue, which affects the quality of RNA or protein extraction.
[0005] To achieve the above objectives, the present application provides a low-temperature tissue grinding device, comprising: a mortar and a grinding rod used in conjunction with the mortar;
[0006] The mortar includes a first shell and a second shell arranged outside the first shell, the first shell encloses a grinding chamber, and the first shell and the second shell enclose an ice water storage chamber; a sample addition port is provided at the top of the mortar, and the sample addition port is connected to the grinding chamber; an ice water injection port is provided on the second shell, and the ice water injection port is connected to the ice water storage chamber.
[0007] Preferably, the bottom of the first shell and the bottom of the second shell are connected.
[0008] Preferably, the grinding rod comprises a hand-held portion and a grinding portion, and the hand-held portion is in a cross shape.
[0009] Preferably, the grinding rod comprises a hand-held portion and a grinding portion, and the hand-held portion has a horizontal U-shaped protrusion.
[0010] Preferably, the difference between the diameter of the grinding chamber and the diameter of the grinding portion is 0.1 mm-0.15 mm.
[0011] Preferably, the diameter of the sample addition port is larger than the diameter of the grinding chamber.
[0012] Preferably, the mortar and the grinding rod are made of borosilicate glass.
[0013] Preferably, the mortar and the grinding rod are made of stainless steel.
[0014] Preferably, the top end of the grinding rod is also provided with an electric drill interface.
[0015] Preferably, the bottom of the mortar is further provided with a trapezoidal base.
[0016] Compared with the prior art, the advantages of this application include:
[0017] The low-temperature tissue grinding device provided in the present application adds an ice water storage chamber outside the grinding chamber of the mortar. Ice water can be added to the ice water storage chamber, so that the heat generated by grinding in the grinding chamber is eliminated in time, so that the tissue sample is always in a low-temperature environment during the grinding process, effectively ensuring that the RNA and protein in the tissue sample are not degraded or degraded as little as possible during the grinding process, thereby ensuring the accuracy of subsequent experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope of the present application.
[0019] Figure 1 A schematic structural diagram of an embodiment of a mortar of a low-temperature tissue grinding device;
[0020] Figure 2 A schematic structural diagram of another embodiment of a mortar of a low-temperature tissue grinding device;
[0021] Figure 3 A schematic structural diagram of an embodiment of a grinding rod of a low-temperature tissue grinding device;
[0022] Figure 4 Schematic diagram of the structure of another embodiment of a grinding rod of a low-temperature tissue grinding device.
[0023] Reference numerals:
[0024] 100-mortar; 10-first shell; 12-grinding chamber; 14-sample addition port; 20-second shell; 22-ice water storage chamber; 24-ice water injection port; 30-trapezoidal base; 200-grinding rod; 210-handheld part; 220-grinding part; 230-electric drill interface. DETAILED DESCRIPTION
[0025] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.
[0026] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. Conversely, when an element is referred to as being "directly on" another element, there is no intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.
[0027] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, indirect connection through an intermediate medium, internal communication between two components, or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.
[0028] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of this utility model, "plurality" means two or more, unless otherwise specifically defined.
[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs. The terms used in the template description herein are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.
[0030] This application provides a low-temperature tissue grinding device, please refer to Figure 1 and Figure 3, including: a mortar 100 and a grinding rod 200 used in conjunction with the mortar 100; the mortar 200 includes a first shell 10 and a second shell 20 sleeved outside the first shell 10, the first shell 10 encloses a grinding chamber 12, and the first shell 10 and the second shell 20 enclose an ice water storage chamber 22; a sample addition port 14 is provided at the top of the mortar 200, and the sample addition port 14 is connected to the grinding chamber 12; an ice water injection port 24 is provided on the second shell 20, and the ice water injection port 24 is connected to the ice water storage chamber 22.
[0031] When the low-temperature tissue grinding device is in use, the tissue sample is added to the grinding chamber 12, ice water is added to the ice water storage chamber 22, and the grinding rod 200 is placed in the grinding chamber 12, so that there is a tiny gap and fine concave-convex bite between the grinding rod 200 and the grinding chamber 12. When the grinding rod 200 is rotated, a grinding and cutting effect is generated, so that biological tissue cells and other relatively hard cell tissues are easily crushed.
[0032] The low-temperature tissue grinding device provided in the present application adds an ice water storage chamber 22 outside the grinding chamber 12 of the mortar 100. Ice water can be added to the ice water storage chamber 22, so that the heat generated by grinding in the grinding chamber 12 is eliminated in time, so that the tissue sample is always in a low-temperature environment during the grinding process, effectively ensuring that the RNA and protein in the tissue sample are not degraded or degraded as little as possible during the grinding process, thereby ensuring the accuracy of subsequent experiments.
[0033] In a preferred embodiment, the bottom of the first shell 10 and the bottom of the second shell 20 are connected. This arrangement allows the grinding chamber 12 to be supported by both the first shell 10 and the second shell 20, thereby improving the stability of the grinding chamber. It is understood that the bottoms of the first shell 10 and the second shell 20 can also be disconnected, so that the grinding chamber 12 is suspended within the mortar 100, supported only by the first shell 10.
[0034] In one embodiment, see Figure 3 The grinding rod 200 includes a handheld portion 210 and a grinding portion 220, wherein the handheld portion 210 is in a cross shape. The cross-shaped handheld portion 210 grinding rod 200 is a traditional grinding rod 200 shape, which is relatively simple to manufacture, but is not easy to rotate and grinding is relatively laborious.
[0035] In another embodiment, see Figure 4 The grinding rod 200 includes a hand-held portion 210 and a grinding portion 220. The hand-held portion 210 has a horizontal U-shaped protrusion. The horizontal U-shaped protrusion on the grinding rod 200 is like a handle, which is convenient to hold and easier to rotate and grind, resulting in better grinding effect and saving time and effort.
[0036] In a preferred embodiment, the difference between the diameter of the grinding chamber 12 and the diameter of the grinding part 220 is 0.1 mm-0.15 mm, that is, the gap between the cavity wall of the grinding chamber 12 and the grinding part 220 is 0.1 mm-0.15 mm. The gap within this range can ensure that the tissue sample is fully ground. If the gap is too small, the tissue sample cannot enter the grinding chamber 12 during grinding. If the gap is too large, the friction is too small and the grinding is insufficient.
[0037] In a preferred embodiment, please continue to refer to Figure 1 The diameter of the sample addition port 14 is larger than the diameter of the grinding chamber 12 to facilitate the addition of sample tissue into the grinding chamber 12.
[0038] In one embodiment, the mortar 100 and the grinding rod 200 are made of borosilicate glass. The visible borosilicate glass material allows for easy observation of the grinding process, ensuring that the tissue sample is ground in one go.
[0039] In another embodiment, the mortar 100 and the grinding rod 200 are made of stainless steel. Stainless steel is more durable than borosilicate glass, increasing the strength of the grinding chamber 12 and making it less susceptible to breakage and injury to the experimenter. Furthermore, stainless steel offers excellent thermal conductivity, facilitating the transfer of ice water temperature to the tissue sample being ground, thus facilitating cryogenic grinding.
[0040] In a preferred embodiment, see Figure 4 The top of the grinding rod 200 is also provided with an electric drill interface 230, so that it can be connected to an electric drill, so that the low-temperature tissue grinding device can be used for manual grinding or directly connected to a hand electric drill for grinding, greatly increasing the grinding efficiency.
[0041] In a preferred embodiment, see Figure 2 The bottom of the mortar 100 is also provided with a trapezoidal base 30. The circular bottom of the existing mortar 100 itself means that the mortar 100 can only be placed upside down on the test bench, which can easily cause leakage and contamination of the ground tissue. By providing an enlarged trapezoidal base 30 at the bottom, the mortar 100 can be conveniently placed vertically on the laboratory table.
[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present application.
[0043] Furthermore, those skilled in the art will appreciate that although some embodiments herein include certain features included in other embodiments but not others, combinations of features from different embodiments are intended to be within the scope of this application and to form distinct embodiments. For example, in the claims above, any of the claimed embodiments may be used in any combination. The information disclosed in this background section is intended solely to enhance understanding of the overall background of this application and should not be construed as an admission or any implication that such information constitutes prior art known to those skilled in the art.
Claims
1. A low-temperature tissue grinding device, characterized in that: include: A mortar and a grinding rod used in conjunction with the mortar; The mortar comprises a first shell and a second shell sleeved outside the first shell, the first shell encloses a grinding chamber, and the first shell and the second shell enclose an ice water storage chamber; The top of the mortar is provided with a sample adding port, which is communicated with the grinding chamber; the second shell is provided with an ice water injection port, which is communicated with the ice water storage chamber.
2. The cryogenic tissue grinding device according to claim 1, characterized in that: The bottom of the first shell is connected to the bottom of the second shell.
3. The cryogenic tissue grinding device according to claim 1, characterized in that: The grinding rod comprises a hand-held portion and a grinding portion, and the hand-held portion is in a cross shape.
4. The cryogenic tissue grinding device according to claim 1, characterized in that: The grinding rod comprises a hand-held portion and a grinding portion, wherein the hand-held portion has a horizontal U-shaped protrusion.
5. The cryogenic tissue grinding device according to claim 3 or 4, characterized in that: The difference between the diameter of the grinding chamber and the diameter of the grinding part is 0.1 mm to 0.15 mm.
6. The cryogenic tissue grinding device according to claim 5, characterized in that: The diameter of the sample addition port is larger than the diameter of the grinding chamber.
7. The cryogenic tissue grinding device according to claim 1, characterized in that: The mortar and the grinding rod are made of high borosilicate glass.
8. The cryogenic tissue grinding device according to claim 1, characterized in that: The mortar and the grinding rod are made of stainless steel.
9. The cryogenic tissue grinding device according to claim 8, characterized in that: The top end of the grinding rod is also provided with an electric drill interface.
10. The cryogenic tissue grinding device according to claim 1, characterized in that: The bottom of the mortar is also provided with a trapezoidal base.