Protein purification analyzer

Abstract

The utility model relates to the technical field of life science experiment equipment, in particular to a protein purification analyzer which comprises an analyzer body, an electric cylinder arranged at the top of the analyzer body, a fluted disc mounted on the lower side of the electric cylinder, an injection head fixedly mounted on one side of the lower surface of the fluted disc, a stirring shaft arranged on one side of the injection head, and stirring blades fixedly connected to the outer ring surface of the stirring shaft. A liquid pumping head is arranged on one side of the stirring blade, a turntable is mounted on the lower side of the liquid pumping head, a limiting sleeve is fixedly mounted on the outer ring surface of the turntable, and a reaction tube is slidably sleeved with the limiting sleeve; the full-automatic purification device has the beneficial effects that the purification volume is increased through technical improvement, the purification of a large-volume sample to be purified can be met, the full-automatic purification device has the characteristics of convenience in use and high purification efficiency, meanwhile, the full-automatic technology is adopted, the full-automatic operation can be realized as long as relevant technical parameters are input into the controller, and the production cost is reduced. The operation difficulty is greatly reduced, the use is convenient, and the protein purification speed can be effectively accelerated.

Description

Technical Field

[0001] The utility model relates to the technical field of life science experimental equipment, in particular to a protein purification analyzer. Background Art

[0002] A protein purification analyzer is a precision instrument specifically designed to monitor and evaluate sample purity, concentration, and molecular properties during the protein purification process. Used in large-scale life science laboratories, it significantly improves purification efficiency and saves significant manpower and resources compared to traditional methods. It can perform a variety of commonly used purification techniques, such as affinity chromatography, ion exchange chromatography, hydrophobic chromatography, and gel filtration.

[0003] In the prior art, traditional protein purification analyzers are usually designed for plate purification, and the use of micro-deep-well 96-well plates is the most common. This design can solve the purification scheme of multiple sample volumes, but the purification volume of each sample is limited by the volume of the well plate, which is generally a maximum of 1 mL / sample, and cannot meet the needs of large-volume sample purification. If a large-volume sample is evenly divided into each well for purification, the purified products of several wells need to be collected and concentrated after purification, which requires a lot of time. Existing protein purification analyzers usually only perform simple magnetic transfer and mixing operations. The preparation of reagents needs to be added to the well plate before purification. During the liquid addition process, due to the large number of wells, it is easy to cause omissions or over-addition, resulting in experimental errors, and it also takes a lot of time. Utility Model Content

[0004] The purpose of the present invention is to provide a protein purification analyzer to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a protein purification analyzer, comprising: an analyzer body, an electric cylinder is arranged on the top of the analyzer body, a gear disk is installed on the lower side of the electric cylinder, an injection head is fixedly installed on one side of the lower surface of the gear disk, a stirring shaft is arranged on one side of the injection head, a stirring blade is fixedly connected to the outer ring surface of the stirring shaft, and a liquid extraction head is arranged on one side of the stirring blade.

[0006] A turntable is installed at the lower side of the liquid extraction head, a limiting sleeve is fixedly installed on the outer ring surface of the turntable, and a sliding sleeve inside the limiting sleeve is provided with a reaction tube.

[0007] Preferably, the top of the electric cylinder is fixedly connected to the top of the fixed frame, the output end of the bottom of the electric cylinder is fixedly connected to the lifting plate, the upper motor is fixedly connected to one side of the lower surface of the lifting plate, and the T-shaped shaft is fixedly connected to the other side of the lower surface of the lifting plate.

[0008] Preferably, a support frame is provided on the lower side of the upper motor, the top of the support frame is fixedly connected to the lifting plate, and a connecting shaft is rotatably sleeved on the bottom of the support frame. One end of the connecting shaft is fixedly connected to the output end of the upper motor, and the other end of the connecting shaft is fixedly sleeved in the pinion.

[0009] Preferably, the pinion is meshed with the gear disc for transmission, the gear disc is rotatably sleeved on the T-shaped shaft, a limit ring is installed on the upper side of the gear disc, the limit ring is fixedly sleeved on the T-shaped shaft, a drive motor is provided on one side of the T-shaped shaft, the output end of the drive motor passes through the gear disc and is threadedly connected to the upper end of the stirring shaft.

[0010] Preferably, a reagent bottle is installed on one side of the driving motor, a T-tube is provided on one side of the reagent bottle, a solenoid valve is fixedly installed on one side of the T-tube, an injection pump is fixedly installed on the other side of the T-tube, a waste liquid pump is fixedly installed on one side of the injection pump, and a waste liquid tank is fixedly installed on the upper side of the waste liquid pump.

[0011] Preferably, a rotating shaft is provided at the bottom of the waste liquid tank, a turntable fixed sleeve is arranged on the top of the rotating shaft, a rotating sleeve is arranged on the bottom of the support sleeve at the bottom of the rotating shaft, a limiting frame is provided on the upper side of the support sleeve, the middle part of the rotating shaft is rotatably sleeved in the limiting frame, and a transmission disc is provided on the upper side of the limiting frame.

[0012] Preferably, a groove is provided on the surface of the transmission disk, and an arc surface is provided on one side of the groove. The arc surface is slidingly connected to one side of the notched disk, and a shift rod is fixedly connected to the other side of the notched disk. The shift rod is slidingly connected to the groove, and the bottom of the notched disk is fixedly connected to the output end of the servo motor. An electric push rod is provided on one side of the servo motor, and the output end of the electric push rod is fixedly connected to a magnetic sleeve.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. The utility model increases the purification volume through technical improvements, which can meet the purification needs of large-volume samples to be purified, and has the characteristics of easy use and high purification efficiency.

[0015] 2. This utility model adopts fully automatic technology. As long as the relevant technical parameters are input into the controller, fully automatic operation can be achieved, which greatly reduces the difficulty of operation, is easy to use, and can effectively speed up the speed of protein purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 This is a bottom view schematic diagram of the overall structure of the utility model;

[0018] Figure 3 This is a schematic top view of the overall structure of the utility model;

[0019] Figure 4 For this utility model Figure 1 A magnified schematic diagram of the structure in the middle.

[0020] In the figure: 1. Analyzer body; 2. Electric cylinder; 3. Gear disk; 4. Injection head; 5. Stirring shaft; 6. Stirring blade; 7. Liquid extraction head; 8. Turntable; 9. Limiting sleeve; 10. Reaction tube; 11. Fixing frame; 12. Lifting plate; 13. Upper motor; 14. T-shaped shaft; 15. Support frame; 16. Connecting shaft; 17. Pinion; 18. Limiting ring; 19. Drive motor; 20. Reagent bottle; 21. T-shaped tube; 22. Solenoid valve; 23. Injection pump; 24. Discarding pump; 25. Waste liquid tank; 26. Rotating shaft; 27. Support sleeve; 28. Limiting frame; 29. ​​Transmission disk; 30. Groove; 31. Arc surface; 32. Notched disk; 33. Push rod; 34. Servo motor; 35. Electric push rod; 36. Magnetic sleeve. DETAILED DESCRIPTION

[0021] In order to clearly and completely describe the purpose and technical solution of the present invention and make its advantages more clearly understood, the following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only part of the embodiments of the present invention, not all of them, and are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1: Please refer to Figures 1-4 The utility model provides a technical solution: a protein purification analyzer, comprising: an analyzer body 1, an electric cylinder 2 is arranged on the top of the analyzer body 1, a gear disk 3 is installed on the lower side of the electric cylinder 2, an injection head 4 is fixedly installed on one side of the lower surface of the gear disk 3, a stirring shaft 5 is arranged on one side of the injection head 4, a stirring blade 6 is fixedly connected to the outer ring surface of the stirring shaft 5, a liquid extraction head 7 is arranged on one side of the stirring blade 6, a turntable 8 is installed on the lower side of the liquid extraction head 7, a limiting sleeve 9 is fixedly installed on the outer ring surface of the turntable 8, and a reaction tube 10 is provided in a sliding sleeve inside the limiting sleeve 9.

[0023] The reaction tube 10 is a 50 ml centrifuge tube, with a total of four, which can simultaneously perform four-channel sample purification. They are all slidably sleeved in the limiting sleeve 9, and the reaction tube 10 is limited by the limiting sleeve 9 to ensure its stability during operation. Different buffer solutions are added to the reagent bottle 20 before use, and the operating time and other programs of each driving component are set. Then the entire device is covered in a transparent sterilization cabin to sterilize each component before purification analysis. When in use, after running the program, the injection head 4 is rotated to the top of the reaction tube 10 by rotating the toothed disc 3, and then the electric cylinder 2 is started. The electric cylinder 2 indirectly drives the injection head 4 to extend into the reaction tube 10, and the reagent in the reagent bottle 20 is injected into the reaction tube 10. After the injection is completed, the electric cylinder 2 moves in the opposite direction, and then the toothed disc 3 is rotated again to move the stirring shaft 5 into the reaction tube 10 where the injection has just been completed. , the electric cylinder 2 moves downward, driving the stirring shaft 5 and the stirring blade 6 to extend into a certain depth below the liquid level of the reaction tube 10, and stirring begins. After the set reaction time is reached, stirring stops, and the stirring shaft 5 is removed by the reverse movement of the electric cylinder 2, and the output end of the electric push rod 35 is controlled to extend, so that it drives the magnetic suction sleeve 36 to move to the bottom of the reaction tube 10 where the magnetic microspheres need to be adsorbed. Through the magnetic effect, the magnetic microspheres are attached to the side wall of the reaction tube 10, and finally the gear disk 3 is driven to rotate again, so that the liquid extraction head 7 is placed just above the reaction tube 10, and the output end of the electric cylinder 2 extends, and the driven liquid extraction head 7 extends into the bottom of the reaction tube 10, and the waste liquid pump 24 is started to pump the waste liquid in the reaction tube 10 into the waste liquid tank 25. After the liquid extraction is completed, the liquid extraction head 7 is removed by the reverse movement of the electric cylinder 2, and the magnetic field is immediately withdrawn, thereby facilitating the next reaction.

[0024] Embodiment 2: On the basis of embodiment 1, the top of the electric cylinder 2 is fixedly connected to the top of the fixing frame 11, the output end of the bottom of the electric cylinder 2 is fixedly connected to the lifting plate 12, one side of the lower surface of the lifting plate 12 is fixedly connected to the upper motor 13, and the other side of the lower surface of the lifting plate 12 is fixedly connected to the T-shaped shaft 14. The fixing frame 11 provides fixed support for the entire device. The non-output end of the electric cylinder 2 is fixedly mounted on the top of the fixing frame 11, and the output end of the electric cylinder 2 is fixedly connected to the upper surface of the lifting plate 12. The electric cylinder 2 is provided with a group of two and is symmetrically arranged about the central plane of the lifting plate 12. One side of the lower surface of the lifting plate 12 is fixed to the upper motor 13, and the other side of the lifting plate 12 is fixed to the T-shaped shaft 14. A support frame 15 is provided on the lower side of the upper motor 13. The support frame The top of 15 is fixedly connected to the lifting plate 12, and the bottom of the support frame 15 is rotatably sleeved with a connecting shaft 16. One end of the connecting shaft 16 is fixedly connected to the output end of the upper motor 13, and the other end of the connecting shaft 16 is fixedly sleeved in the pinion 17. The support frame 15 is fixedly installed on the same side of the upper motor 13 and is fixedly connected to the lifting plate 12. The support frame 15 limits the connecting shaft 16, thereby ensuring that the upper motor 13 drives the connecting shaft 16 to rotate stably, so that the connecting shaft 16 drives the pinion 17 to rotate stably, and the pinion 17 is engaged with the gear disc 3 for transmission. The gear disc 3 is rotatably sleeved on the T-shaped shaft 14, and a limiting ring 18 is installed on the upper side of the gear disc 3. The limiting ring 18 is fixedly sleeved on the T-shaped shaft 14. A driving motor 19 is provided on one side of the T-shaped shaft 14. The driving motor The output end 19 passes through the gear disc 3 and is threadedly connected to the upper end of the stirring shaft 5. The pinion 17 meshes with the gear disc 3 for transmission, thereby driving the gear disc 3 to rotate. The gear disc 3 limits the bottom of the gear disc 3 by the T-axis 14 and the top of the gear disc 3 by the limiting ring 18, thereby ensuring the smooth rotation of the gear disc 3. The driving motor 19 is fixedly mounted on the upper surface of the gear disc 3. The output end of the driving motor 19 is threadedly connected to the top of the stirring shaft 5, so that after one purification is completed, the stirring blade 6 can be replaced by replacing the stirring shaft 5, thereby effectively avoiding the problem of contamination. A reagent bottle 20 is installed on one side of the driving motor 19, and a T-tube 21 is provided on one side of the reagent bottle 20. A solenoid valve 22 is fixedly installed on one side of the T-tube 21, and a solenoid valve 22 is fixedly installed on the other side of the T-tube 21. It is equipped with an injection pump 23, a waste liquid pump 24 is fixedly installed on one side of the injection pump 23, a waste liquid tank 25 is fixedly installed on the upper side of the waste liquid pump 24, the reagent bottle 20 is fixedly installed on the upper surface of the toothed disc 3, the reagent bottle 20 is fixedly installed on the upper surface of the toothed disc 3, the lower end of the T-tube 21 is fixedly sleeved with the input end of the injection pump 23 and is connected with the input end, the side end of the T-tube 21 is fixedly sleeved with a solenoid valve 22, and the solenoid valve 22 is arranged in a linear array, the input end of the solenoid valve 22 extends into the reagent bottle 20 through a hose, the output end of the solenoid valve 22 is connected with the T-tube 21, the output end of the injection pump 23 is connected with the injection head 4 through a connecting pipe, the input end of the waste liquid pump 24 is connected with the liquid extraction head 7 through a suction tube, and the input end of the waste liquid pump 24 is connected with the waste liquid tank 25.

[0025] Before use, different buffer solutions are added to the reagent bottle 20, and the reaction volume, reaction time and other programs are set. Then, the fixed frame 11 is placed in a transparent sterilization box, and the program is run. The driving connecting shaft 16 of the upper motor 13 rotates under the limit of the support frame 15, so that the connecting shaft 16 drives the small gear 17 to rotate, and the small gear 17 engages with the gear plate 3 for transmission, thereby driving the gear plate 3 to rotate. The gear plate 3 adjusts the position of the injection head 4 so that it can be placed just above the reaction tube 10, and the electric cylinder 2 is started to move downward, so that the electric cylinder 2 passes through the belt. The movable lifting plate 12 moves downward to indirectly drive the injection head 4 into the reaction tube 10, start the injection pump 23, and at the same time open one solenoid valve 22, while the other solenoid valves 22 are closed, and the reagent in one of the reagent bottles 20 is pumped into the reaction tube 10. After the injection is completed, the electric cylinder 2 moves in the opposite direction, and the injection head 4 moves away from the reaction tube 10. Then, the gear 3 is meshed with the pinion 17 to rotate the gear 3, and the stirring shaft 5 is moved to the top of the reaction tube 10. The electric cylinder 2 is started to move downward, so that the stirring shaft 5 is moved to the top of the reaction tube 10 according to the reverse direction set in the reaction tube 10. According to the volume, the stirring shaft 5 is extended downward to a certain depth below the liquid level of the reaction tube 10, and stirring is started. After the set reaction time is reached, the stirring is stopped, and the stirring shaft 5 is removed by raising the electric cylinder 2. Then, the electric push rod 35 is extended, and its output end drives the magnetic sleeve 36 to approach the reaction tube 10. The inner ring surface of the magnetic sleeve 36 is provided with a magnetic block. Through the magnetic effect, the magnetic microspheres are attached to the tube wall of the reaction tube 10. At the same time, the gear 3 is once again driven by the meshing transmission of the pinion 17 and the toothed disc 3, so that the toothed disc 3 drives the pump. The liquid head 7 moves to the top of the reaction tube 10, and the electric cylinder 2 indirectly drives the liquid extraction head 7 to extend into the bottom of the reaction tube 10, starts the waste liquid pump 24, and pumps the waste liquid in the reaction tube 10 into the waste liquid tank 25, and contracts the electric push rod 35 to remove the magnetic field to prevent the magnetic microspheres from agglomerating in the magnetic field for a long time. Then, the stirring shaft 5 is replaced, the initially started solenoid valve 22 is closed, and another solenoid valve 22 is started, thereby switching to another reagent bottle 20, thereby facilitating the next reaction, and so on and so forth until the purification process is completed.

[0026] Example 3: On the basis of Example 2, a rotating shaft 26 is provided at the bottom of the waste liquid tank 25, and the turntable 8 is fixedly sleeved on the top of the rotating shaft 26. The bottom of the rotating shaft 26 is rotatably sleeved on the bottom of the support sleeve 27. A limiting frame 28 is provided on the upper side of the support sleeve 27. The middle part of the rotating shaft 26 is rotatably sleeved in the limiting frame 28. A transmission disk 29 is provided on the upper side of the limiting frame 28. The bottom of the rotating shaft 26 is limited by the support sleeve 27. The middle part of the rotating shaft 26 is stabilized by the limiting frame 28. The top of the rotating shaft 26 is fixedly sleeved with a rotating disc 8. The limiting frame 28 is fixedly mounted on the bottom of the fixed frame 11. The transmission disk 29 9 has a groove 30 on its surface, and an arc surface 31 is provided on one side of the groove 30. The arc surface 31 is slidably connected to one side of the notched disk 32. A lever 33 is fixedly connected to the other side of the notched disk 32. The lever 33 is slidably connected to the groove 30. The bottom of the notched disk 32 is fixedly connected to the output end of the servo motor 34. An electric push rod 35 is provided on one side of the servo motor 34. The output end of the electric push rod 35 is fixedly connected to a magnetic sleeve 36. The grooves 30 and the arc surface 31 on the surface of the transmission disk 29 are arranged in a circular array, and the servo motor 34 is fixedly mounted on the bottom of the fixed frame 11.

[0027] When a reaction tube 10 completes a reaction, when the next reaction tube 10 needs to be operated, the servo motor 34 is started, and the servo motor 34 drives the notched disk 32 to rotate. Under the rotation of the notched disk 32, the outer ring surface of the notched disk 32 first slides between the arc surface 31. At this time, the transmission disk 29 does not rotate, and the notched disk 32 continues to rotate, causing the lever 33 to slide into the groove 30, thereby driving the transmission disk 29 to rotate through the sliding connection between the lever 33 and the groove 30. The transmission disk 29 then drives the turntable 8 to rotate under the connection action of the rotating shaft 26, so that the turntable 8 drives the next reaction tube 10 to rotate ninety degrees, so that it rotates to just above the magnetic suction sleeve 36, and the servo motor 34 stops moving, and then the next reaction is carried out. This reciprocating process is repeated until the purification process is completed.

[0028] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A protein purification analyzer, comprising an analyzer body (1), characterized in that: The analyzer body (1) is provided with an electric cylinder (2) on the top, a toothed disc (3) is installed on the lower side of the electric cylinder (2), an injection head (4) is fixedly installed on one side of the lower surface of the toothed disc (3), a stirring shaft (5) is provided on one side of the injection head (4), a stirring blade (6) is fixedly connected to the outer ring surface of the stirring shaft (5), and a liquid extraction head (7) is provided on one side of the stirring blade (6); A turntable (8) is installed on the lower side of the liquid extraction head (7), a limiting sleeve (9) is fixedly installed on the outer ring surface of the turntable (8), and a reaction tube (10) is provided in a sliding sleeve inside the limiting sleeve (9).

2. A protein purification analyzer according to claim 1, characterized in that: The top of the electric cylinder (2) is fixedly connected to the top of the fixing frame (11), the output end of the bottom of the electric cylinder (2) is fixedly connected to the lifting plate (12), one side of the lower surface of the lifting plate (12) is fixedly connected to the upper motor (13), and the other side of the lower surface of the lifting plate (12) is fixedly connected to the T-shaped shaft (14).

3. A protein purification analyzer according to claim 2, characterized in that: A support frame (15) is provided on the lower side of the upper motor (13), the top of the support frame (15) is fixedly connected to the lifting plate (12), and a connecting shaft (16) is rotatably sleeved on the bottom of the support frame (15), one end of the connecting shaft (16) is fixedly connected to the output end of the upper motor (13), and the other end of the connecting shaft (16) is fixedly sleeved in the pinion (17).

4. A protein purification analyzer according to claim 3, characterized in that: The pinion (17) is meshed with the toothed disc (3) for transmission. The toothed disc (3) is rotatably sleeved on the T-shaped shaft (14). A limit ring (18) is installed on the upper side of the toothed disc (3). The limit ring (18) is fixedly sleeved on the T-shaped shaft (14). A drive motor (19) is provided on one side of the T-shaped shaft (14). The output end of the drive motor (19) passes through the toothed disc (3) and is threadedly connected to the upper end of the stirring shaft (5).

5. A protein purification analyzer according to claim 4, characterized in that: A reagent bottle (20) is installed on one side of the driving motor (19), a T-shaped tube (21) is provided on one side of the reagent bottle (20), a solenoid valve (22) is fixedly installed on one side of the T-shaped tube (21), an injection pump (23) is fixedly installed on the other side of the T-shaped tube (21), a waste liquid pump (24) is fixedly installed on one side of the injection pump (23), and a waste liquid tank (25) is fixedly installed on the upper side of the waste liquid pump (24).

6. A protein purification analyzer according to claim 5, characterized in that: A rotating shaft (26) is provided at the bottom of the waste liquid tank (25), a rotating disc (8) is fixedly sleeved on the top of the rotating shaft (26), the bottom of the rotating shaft (26) is rotatably sleeved on the bottom of the supporting sleeve (27), a limiting frame (28) is provided on the upper side of the supporting sleeve (27), the middle part of the rotating shaft (26) is rotatably sleeved in the limiting frame (28), and a transmission disc (29) is provided on the upper side of the limiting frame (28).

7. A protein purification analyzer according to claim 6, characterized in that: The transmission disk (29) has a groove (30) on its surface, and an arc surface (31) is provided on one side of the groove (30). The arc surface (31) is slidably connected to one side of the notched disk (32). The other side of the notched disk (32) is fixedly connected to a lever (33). The lever (33) is slidably connected to the groove (30). The bottom of the notched disk (32) is fixedly connected to the output end of the servo motor (34). An electric push rod (35) is provided on one side of the servo motor (34), and the output end of the electric push rod (35) is fixedly connected to a magnetic suction sleeve (36).

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