Nucleic acid collection tube and multi-pathogen nucleic acid detection device

Abstract

A nucleic acid collecting tube comprises a test tube, a tube cover and an inner cover, the upper end of the test tube is open, the outer wall of the annular wall of the inner cover is in threaded connection with the inner wall of the test tube, the test tube and the inner cover jointly form a sealed tube cavity, a second through hole communicated with the tube cavity is formed in the inner cover, and the tube cover is arranged at the upper end of the test tube. A first through hole is formed in the top wall of the tube cover, the tube cover can rotate relative to the test tube, a second through hole is formed between the circle center of the inner cover and the edge of the inner cover, and the diameter of the second through hole is equal to that of the first through hole; the distance from the circle center of the second through hole to the axis of the test tube is equal to the distance from the circle center of the first through hole to the axis of the test tube, the tube cover rotates relative to the test tube until the first through hole and the second through hole do not intersect, and the tube cover, the inner cover and the test tube form a sealed tube cavity. The utility model further provides a multi-pathogen nucleic acid detection device.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, in particular to a nucleic acid collection tube and a multi-pathogen nucleic acid detection device. Background Art

[0002] As the first line of defense against imported cases, customs has an important task of improving nucleic acid testing capabilities to meet the testing requirements of incoming people and animals (such as sheep).

[0003] In existing technology, after collecting a nucleic acid sample, the tube cap must be removed and a cotton swab inserted into the tube, with the head of the swab resting against one side of the tube and the middle of the swab against the other side. The base of the swab is then pressed down, breaking off the head inside the tube. The cap is then screwed back on and the sample is sent for testing. The removed tube cap is often temporarily placed on a table, where it can easily become contaminated and affect test results.

[0004] A Chinese utility model with authorization publication number CN218356248U discloses a nucleic acid collection tube. The nucleic acid collection tube includes a tube body and a cover. The cover is sealed on the tube body and includes a cover body, a fixed cover, and a rotating cover. The cover body is fixedly connected to the tube body, and a through-hole is provided on the cover body. The fixed cover seals a portion of the through-hole. The rotating cover is rotatably mounted on the cover body and can be rotated to close or open the other portion of the through-hole. After nucleic acid sampling, the rotating cover is rotated open, and the swab is inserted into the tube body through the through-hole and broken. The rotating cover is then rotated to close the through-hole. The rotating cover of the nucleic acid collection tube does not separate from the cover body when it is rotated open, thus avoiding contamination.

[0005] When the through hole of the above utility model is closed by the rotating cover, the contact area between the fixed cover and the rotating cover is small, and the sealing effect of the inner cavity of the tube body is poor. Summary of the Invention

[0006] In view of this, it is necessary to propose a nucleic acid collection tube to address the above-mentioned shortcomings.

[0007] It is also necessary to propose a multi-pathogen nucleic acid detection device.

[0008] A nucleic acid collection tube comprises a test tube, a tube cover, and an inner cover. The upper end of the test tube is open, and the lower end of the test tube is closed. The outer wall of the annular wall of the inner cover is threadedly connected to the inner wall of the test tube. The upper end surface of the inner cover is flush with the upper end surface of the test tube. The test tube and the inner cover together form a sealed tube cavity. A second through hole communicating with the tube cavity is provided on the inner cover. The tube cover is provided at the upper end of the test tube, and a first through hole is provided on the top wall of the tube cover. The tube cover can rotate relative to the test tube. The tube cover and the test tube are coaxially arranged. The second through hole is located between the center of the inner cover and the edge of the inner cover. The diameter of the second through hole is equal to the diameter of the first through hole, and the distance from the center of the second through hole to the axis of the test tube is equal to the distance from the center of the first through hole to the axis of the test tube.

[0009] Preferably, the inner wall of the ring wall of the tube cover is threadedly connected to the outer wall of the test tube.

[0010] Preferably, the inner wall of the annular wall of the tube cover is provided with a first friction layer, and the outer wall of the test tube is provided with a second friction layer, and the first friction layer and the second friction layer are in contact.

[0011] Preferably, the lower end surface of the tube cover is a smooth plane.

[0012] Preferably, the upper end surface of the inner cover is a smooth plane.

[0013] Preferably, the lumen is spiral-shaped.

[0014] A multi-pathogen nucleic acid detection device comprises a nucleic acid collection tube, wherein a suction cup is provided at the bottom of the tube.

[0015] Preferably, the multi-pathogen nucleic acid detection device further comprises a storage disk, which is a rectangular plate, and the upper surface of the storage disk is a smooth plane so that the test tube can be fixed to the upper surface of the storage disk by a suction cup.

[0016] Preferably, the multi-pathogen nucleic acid detection device further includes a droplet generator.

[0017] Preferably, the multi-pathogen nucleic acid detection device further includes a droplet analyzer.

[0018] Beneficial effect: When the tube cover rotates relative to the test tube until the first through hole and the second through hole have no intersection, the tube cover, the inner cover and the test tube form a sealed tube cavity. Compared with the existing technology, the tube cover and the inner cover have a larger contact area and the sealing effect is better. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is an axonometric view of the nucleic acid collection tube.

[0020] Figure 2 This is a partially disassembled view of the nucleic acid collection tube.

[0021] Figure 3 Axonometric view of a nucleic acid collection tube with attached end caps.

[0022] Figure 4 A partial magnified view of a nucleic acid collection tube with an attached end cap.

[0023] In the figure: test tube 10, tube cover 20, first through hole 21, end cover 30, inner cover 40, second through hole 41, first rotating base 60, second rotating base 70, protrusion 80. DETAILED DESCRIPTION

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, a brief introduction will be given below to the drawings required for use in the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] See also Figure 1 and Figure 2 The embodiment of the present invention provides a nucleic acid collection tube, comprising a test tube 10, a tube cover 20, and an inner cover 40. The upper end of the test tube 10 is open, and the lower end of the test tube 10 is closed. The outer wall of the ring wall of the inner cover 40 is threadedly connected to the inner wall of the test tube 10. The upper end surface of the inner cover 40 is flush with the upper end surface of the test tube 10. The test tube 10 and the inner cover 40 together form a sealed tube cavity. A second through hole 41 communicating with the tube cavity is provided on the inner cover 40. The tube cover 20 is provided on the test tube 10. At the upper end of the tube 10, a first through hole 21 is provided on the top wall of the tube cover 20. The tube cover 20 can rotate relative to the test tube 10. The tube cover 20 and the test tube 10 are coaxially arranged. The second through hole 41 is located between the center of the inner cover 40 and the edge of the inner cover 40. The diameter of the second through hole 41 is equal to the diameter of the first through hole 21. The distance from the center of the second through hole 41 to the axis of the test tube 10 is equal to the distance from the center of the first through hole 21 to the axis of the test tube 10.

[0026] Specifically, the test tube 10 , the tube cover 20 , and the inner cover 30 are coaxially arranged.

[0027] The tube cover 20 rotates relative to the test tube 10 until the first through hole 21 and the second through hole 41 are coaxial. The tube cavity is connected to the outside through the coaxial first through hole 21 and the second through hole 41 so that a cotton swab can be inserted into the test tube 10.

[0028] Beneficial effect: The tube cover 20 rotates relative to the test tube 10 until the first through hole 21 and the second through hole 41 have no intersection, and the tube cover 20, the inner cover 40, and the test tube 10 form a sealed tube cavity. Compared with the existing technology, the tube cover 20 and the inner cover 40 have a larger contact area and a better sealing effect; the tube cover 20 and the inner cover 40 rotate relative to each other, which makes it convenient for the operator to control the opening and closing of the nucleic acid collection tube with one hand, and can also cut and break the cotton swab.

[0029] See also Figure 1 and Figure 2 Furthermore, the inner wall of the annular wall of the tube cover 20 is threadedly connected to the outer wall of the test tube 10. The thread parameter design of the tube cover 20 and the test tube 10, as well as the thread parameter design of the inner cover 40 and the test tube 10, can satisfy that when the lower end surface of the tube cover 20 and the upper end surface of the inner cover 40 are in close contact, the first through hole 21 and the second through hole 41 have no intersection. The specific design parameters are conventional means and are not described in detail. The so-called "no intersection" refers to, for example, dividing the cross-sectional circle of the test tube 10 into four quadrants, with the first through hole 21 in the first quadrant and the second through hole 41 in the third quadrant.

[0030] See also Figure 1 and Figure 2 Furthermore, a first friction layer is provided on the inner wall of the annular wall of the tube cap 20, and a second friction layer is provided on the outer wall of the test tube 10. The first and second friction layers are in contact. The friction between the tube cap 20 and the test tube 10 creates a certain amount of pressure on the inner cap 40, strengthening the seal between the tube cap 20 and the inner cap 40. A certain amount of pulling force is required to separate the tube cap 20 from the test tube 10. The tube cap 20 and the test tube 10 are easily opened and closed, eliminating the need for a threaded connection structure and reducing manufacturing costs.

[0031] See also Figure 1 and Figure 2 Furthermore, the lower end surface of the tube cover 20 is a smooth plane.

[0032] See also Figure 1 and Figure 2 Furthermore, the upper end surface of the inner cover 40 is a smooth plane.

[0033] Furthermore, the lumen is spiral-shaped. After the cotton swab enters the spiral lumen and is broken, the collected material on the cotton swab is not likely to splash out of the lumen.

[0034] The embodiment of the present utility model provides a multi-pathogen nucleic acid detection device, which includes a nucleic acid collection tube, and a suction cup is provided at the bottom of the test tube 10.

[0035] Furthermore, the multi-pathogen nucleic acid detection device also includes a storage disk, which is a rectangular plate. The upper surface of the storage disk is a smooth plane so that the test tube 10 can be fixed to the upper surface of the storage disk through a suction cup.

[0036] Furthermore, the multi-pathogen nucleic acid detection device also includes a droplet generator.

[0037] Furthermore, the multi-pathogen nucleic acid detection device also includes a droplet analyzer.

[0038] The nucleic acid collection tube is used to collect nucleic acid specimens; the storage tray is used to place several completed nucleic acid collection tubes; the transfer box is used to load the nucleic acid collection tubes loaded on the storage tray and send them to a designated location for extraction to prepare a reaction solution; the droplet generator is used to generate 20,000 nanoliter-sized droplets from the reaction solution, each droplet containing or not containing a nucleic acid target molecule. Each droplet acts as an independent PCR reactor and then undergoes PCR amplification; the droplet analyzer is used to detect each droplet one by one, with the presence of a fluorescent signal being interpreted as "1" and the absence of a fluorescent signal being interpreted as "0". Finally, based on the Poisson distribution principle and the proportion of positive droplets, the analysis software calculates the concentration of the target molecule to be tested.

[0039] The droplet generator is specifically a QX200 droplet generator, and the droplet analyzer is specifically a QX200 droplet analyzer. The QX200 droplet analyzer and the QX200 droplet generator constitute the QX200 Droplet Digital PCR system.

[0040] The droplet generator set in the multi-pathogen nucleic acid detection device can achieve absolute quantification and multiple detection. Multiple detection can be understood as the simultaneous detection of multiple pathogens. The detection process is fast and highly accurate. The droplet generator set in the multi-pathogen nucleic acid detection device can realize the digitization and visualization of the detection results.

[0041] See also Figure 3 and Figure 4 In one embodiment, the upper end surface of the tube cover 20 is a smooth plane, and the upper end surface of the tube cover 20 is provided with an end cover 30. The lower end surface of the end cover 30 is a smooth plane. The end cover 30 rotates relative to the tube cover 20, and the lower end surface of the end cover 30 is in contact with the upper end surface of the tube cover 20 to cover the first through hole 21. The rotation axis of the end cover 30 is perpendicular to the axis of the tube cover 20.

[0042] See also Figure 3 and Figure 4 In one embodiment, the nucleic acid collection tube includes a first rotating seat 60 and a second rotating seat 70. The first rotating seat 60 is fixed to the upper end surface of the tube cover 20. The first rotating seat 60 is cylindrical, and the axis direction of the first rotation is parallel to the upper end surface of the tube cover 20. The second rotating seat 70 is fixed to one side of the end cover 30. The second rotating seat 70 is cylindrical, and the axis direction of the second rotating seat 70 is parallel to the lower end surface of the end cover 30. The first rotating seat 60 and the second rotating seat 70 are coaxially rotatably connected. The outer diameters of the first rotating seat 60 and the second rotating seat 70 are equal. There are two first rotating seats 60, and the second rotating seat 70 is arranged between the two first rotating seats 60.

[0043] See also Figure 3 and Figure 4In one embodiment, the thickness of the end cap 30 is equal to the outer diameter of the second rotating seat 70. Exemplarily, the end cap 30 is a plate of uniform thickness, and the planes on which the upper and lower end surfaces of the end cap 30 lie are tangent to the annular wall of the second rotating seat 70. The axes of the first rotating seat 60 and the second rotating seat 70 are both within the midplane between the upper and lower end surfaces of the end cap 30. The distance between the axes of the first rotating seat 60 and the second rotating seat 70 and the upper end surface of the pipe cover 20 is equal to half the thickness of the end cap 30. The connection method between the first rotating seat 60 and the pipe cover 20, and the connection method between the second rotating seat 70 and the end cap 30 are both conventional means and will not be described in detail.

[0044] See also Figure 3 and Figure 4 In one embodiment, a third friction layer is provided on the end surface of the second rotating seat 70, and a fourth friction layer is provided on the end surface of the first rotating seat 60. The third friction layer and the fourth friction layer are in contact, so that when the second rotating seat 70 rotates relative to the first rotating seat 60, a sliding friction resistance is formed between the first rotating seat 60 and the second rotating seat 70, thereby hindering the end cover 30 from rotating relative to the pipe cover 20 and strengthening the sealing of the end cover 30 on the pipe cover 20.

[0045] See also Figure 3 and Figure 4 In one embodiment, a protrusion 80 is provided on one side of the end cap 30. For example, the protrusion 80 is provided on the side of the end cap 30 that is connected to the second rotating base 70. To facilitate installation, the end face of the end cap 30 is designed to be fan-shaped, with a flat sidewall on one side connected to the second rotating base 70 and a protrusion 80 on the side opposite the flat sidewall, making it easier for the operator to open the end cap 30.

[0046] The modules or units in the device of the embodiment of the present utility model can be combined, divided and deleted according to actual needs.

[0047] The above disclosure is only a preferred embodiment of the present invention, and it is certainly not intended to limit the scope of the rights of the present invention. A person skilled in the art can understand that all or part of the processes of the above embodiment and equivalent changes made in accordance with the claims of the present invention are still within the scope of the present invention.

Claims

1. A nucleic acid collection tube, characterized in that: The test tube comprises a test tube, a tube cover and an inner cover, wherein the upper end of the test tube is open and the lower end of the test tube is closed, the outer wall of the annular wall of the inner cover is threadedly connected to the inner wall of the test tube, the upper end surface of the inner cover is flush with the upper end surface of the test tube, the test tube and the inner cover together form a sealed tube cavity, a second through hole communicating with the tube cavity is provided on the inner cover, the tube cover is provided at the upper end of the test tube, a first through hole is provided on the top wall of the tube cover, the tube cover can be rotated relative to the test tube, the tube cover and the test tube are coaxially arranged, the second through hole is located between the center of the inner cover and the edge of the inner cover, the diameter of the second through hole is equal to the diameter of the first through hole, and the distance from the center of the second through hole to the axis of the test tube is equal to the distance from the center of the first through hole to the axis of the test tube.

2. The nucleic acid collection tube according to claim 1, wherein: The inner wall of the ring wall of the tube cover is threadedly connected to the outer wall of the test tube.

3. The nucleic acid collection tube according to claim 1, wherein: The inner wall of the annular wall of the tube cover is provided with a first friction layer, and the outer wall of the test tube is provided with a second friction layer, and the first friction layer is in contact with the second friction layer.

4. The nucleic acid collection tube according to claim 1, wherein: The lower end surface of the tube cover is a smooth plane.

5. The nucleic acid collection tube according to claim 1, wherein: The upper end surface of the inner cover is a smooth plane.

6. The nucleic acid collection tube according to claim 1, wherein: The lumen is spiral-shaped.

7. A multi-pathogen nucleic acid detection device, characterized by: It comprises the nucleic acid collection tube as described in claim 1, wherein a suction cup is provided at the bottom of the tube.

8. The multi-pathogen nucleic acid detection device according to claim 7, wherein: The multi-pathogen nucleic acid detection device also includes a storage disk, which is a rectangular plate. The upper surface of the storage disk is a smooth plane so that the test tube can be fixed to the upper surface of the storage disk through a suction cup.

9. The multi-pathogen nucleic acid detection device according to claim 8, wherein: The multi-pathogen nucleic acid detection device also includes a droplet generator.

10. The multi-pathogen nucleic acid detection device according to claim 9, wherein: The multi-pathogen nucleic acid detection device also includes a droplet analyzer.

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