Biological cell separation device

Abstract

The utility model discloses a biological cell separation device which comprises a separation placing box, the bottom of the separation placing box is fixedly connected with an equipment box, the interior of the equipment box is fixedly connected with a motor, the top of an output shaft of the motor is fixedly connected with a circular end seat, and the top of the circular end seat is fixedly connected with a circular base plate. And the top of the circular base plate is fixedly connected with a circular seat, a placing mechanism is arranged at the top of the circular seat, and a lifting mechanism is arranged at the top of the circular seat. According to the centrifugal tube clamping device, the circular base plate, the circular seat, the rectangular rod, the top seat, the placing holes, the silica gel ring and the circular ring are arranged, the placing holes in the top seat and the placing holes in the circular seat are staggered, the effect of synchronously clamping a plurality of centrifugal tubes is achieved, operation is convenient, and meanwhile the situation that the centrifugal tubes shake in the subsequent separation process is avoided; the rectangular sleeve moves up and down on the rectangular rod, and the limiting height of the top seat to the centrifugal tube is adjusted, so that the gravity center position of the centrifugal tube is conveniently adjusted.

Description

Technical Field

[0001] The utility model relates to the technical field of separation equipment, in particular to a biological cell separation device. Background Art

[0002] Biological cell separation refers to the process of extracting target cells from a tissue after dispersing it into a cell suspension. Biological cell centrifugation is a method that utilizes centrifugal force. The high angular velocity generated by a centrifuge or other equipment makes the centrifugal force much greater than gravity, thus facilitating the precipitation of suspended matter in the solution.

[0003] In the prior art, when the separation device is actually used, the centrifuge tubes need to be placed one by one on the separation plate inside the separation device. Since the placement holes distributed on the separation plate need to be larger than the diameter of the centrifuge tubes, it is impossible to limit the centrifuge tubes of different thicknesses. At the same time, when placing centrifuge tubes of different lengths, the center of gravity position is easily shifted upward, resulting in instability during centrifugal separation. Utility Model Content

[0004] The purpose of the utility model is to solve the shortcomings of the prior art and to provide a biological cell separation device.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A biological cell separation device comprises a separation and placement box, the bottom of the separation and placement box is fixedly connected to an equipment box, the interior of the equipment box is fixedly connected to a motor, the top of the motor output shaft is fixedly connected to a circular end seat, the top of the circular end seat is fixedly connected to a circular pad, the top of the circular pad is fixedly connected to a round seat, the top of the round seat is provided with a placement mechanism, the placement mechanism comprises a rectangular rod, the top of the round seat is fixedly connected to the bottom of the rectangular rod, the outer wall of the rectangular rod is provided with a top seat, a plurality of placement holes are evenly opened on the top seat and the outer wall of the round seat, the inner walls of the plurality of placement holes are fixedly connected to silicone rings, the top of the round seat is rotatably connected to a ring, and by setting the placement mechanism, centrifuge tubes of different thicknesses but of the same batch can be fixed, and the top of the round seat is provided with a lifting mechanism.

[0007] Preferably, the lifting mechanism includes a rectangular sleeve, the top of the circular ring is fixedly connected to the bottom of the rectangular sleeve, the outer wall of the rectangular sleeve is provided with a groove, the inner wall of the groove is slidably connected to a disc, the outer walls of the rectangular sleeve and the circular ring are provided with threaded holes, the inner walls of the two threaded holes are threadedly connected with bolts, one end of one of the bolts is fixedly connected to the outer wall of the disc, and one end of the other bolt is squeezed against the top of the top seat, and the height of the top seat is adjusted by setting the lifting function to facilitate adjustment of the center of gravity position of centrifuge tubes of different lengths.

[0008] Preferably, a through hole is provided at the bottom of the separation and placement box, the inner wall of the through hole is rotatably connected to the outer wall of the motor output shaft, and the circular pad and the multiple centrifuge tubes are driven to rotate by the motor to form a centrifugal effect.

[0009] Preferably, the bottom of the circular end seat is rotatably inlaid with multiple balls, and the outer walls of the multiple balls are in contact with the bottom inner wall of the separation box. By arranging multiple balls, the friction between the circular end seat and the separation box is reduced.

[0010] Preferably, the inner wall of the rectangular sleeve is slidably connected to the outer wall of the rectangular rod, and the outer wall of the disc is squeezed against the outer wall of the rectangular rod. The squeezing force between the disc and the rectangular rod is achieved by rotating the bolt, which can prevent the rectangular sleeve from moving up and down during centrifugation.

[0011] Preferably, a box cover is hinged on the top of the separation storage box, and an electromechanical integrated lock is installed between the box cover and the separation storage box. The electromechanical integrated lock adopts an existing lock body and lock tongue.

[0012] Compared with the prior art, the advantages of the present invention are:

[0013] This solution is provided with a circular pad, a round seat, a rectangular rod, a top seat, a placement hole, a silicone ring and a circular ring. The multiple placement holes on the top seat and the multiple placement holes on the round seat are staggered to form a synchronous clamping effect for multiple centrifuge tubes, which is convenient for operation and avoids the shaking of multiple centrifuge tubes during subsequent separation; the rectangular sleeve is moved up and down on the rectangular rod to adjust the limit height of the top seat on the centrifuge tube, so as to facilitate the adjustment of the center of gravity position of the centrifuge tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the description of the specific implementation methods. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0015] Figure 1 This is a schematic diagram of the three-dimensional structure of a biological cell separation device proposed by the present invention;

[0016] Figure 2 This is a schematic cross-sectional view of a biological cell separation device proposed in the present invention;

[0017] Figure 3 A biological cell separation device proposed in this utility model Figure 2 A schematic diagram of the enlarged structure of part A;

[0018] Figure 4This is a partial three-dimensional structural diagram of a biological cell separation device proposed by the present invention.

[0019] In the figure: 1. Separation placement box; 2. Equipment box; 3. Motor; 4. Round end seat; 5. Ball; 6. Round pad; 7. Round seat; 8. Rectangular rod; 9. Top seat; 10. Placement hole; 11. Silicone ring; 12. Ring; 13. Rectangular sleeve; 14. Round piece; 15. Bolt; 16. Box cover. DETAILED DESCRIPTION

[0020] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] Depend on Figures 1-4 As shown, a biological cell separation device is provided, comprising a separation and placement box 1, the bottom of the separation and placement box 1 is fixedly connected to an equipment box 2, the interior of the equipment box 2 is fixedly connected to a motor 3, the equipment box 2 protects the motor 3, a through hole is provided at the bottom of the separation and placement box 1, the inner wall of the through hole is rotatably connected to the outer wall of the output shaft of the motor 3, the top of the output shaft of the motor 3 is fixedly connected to a circular end seat 4, and the operation of the motor 3 drives the circular end seat 4 to rotate.

[0022] The bottom of the circular end seat 4 is rotatably inlaid with multiple balls 5, and the outer walls of the multiple balls 5 are in contact with the bottom inner wall of the separation and placement box 1. The top of the circular end seat 4 is fixedly connected to a circular pad 6, and the top of the circular pad 6 is fixedly connected to a round seat 7. The circular end seat 4 and the circular pad 6 support the round seat 7.

[0023] A placement mechanism is provided at the top of the round seat 7, which includes a rectangular rod 8. The top of the round seat 7 is fixedly connected to the bottom of the rectangular rod 8. The outer wall of the rectangular rod 8 is provided with a top seat 9. A plurality of placement holes 10 are evenly opened on the top seat 9 and the outer wall of the round seat 7. The inner walls of the plurality of placement holes 10 are fixedly connected with silicone rings 11. The silicone rings 11 are soft, increase the contact area with the centrifuge tube, and have anti-slip capabilities. The top of the round seat 7 is rotatably connected to a ring 12 through an existing bearing. The outer ring of the bearing is fixedly connected to the top of the round seat 7, and the inner ring of the bearing is fixedly connected to the bottom of the ring 12. The bearing is fixedly embedded in the center position of the top seat 9.

[0024] A lifting mechanism is provided at the top of the round seat 7, which includes a rectangular sleeve 13. The inner wall of the rectangular sleeve 13 is slidably connected to the outer wall of the rectangular rod 8. The rectangular sleeve 13 is lifted and lowered by the rectangular rod 8. The outer wall of the disc 14 is squeezed against the outer wall of the rectangular rod 8. The top of the ring 12 is fixedly connected to the bottom of the rectangular sleeve 13. A groove is provided on the outer wall of the rectangular sleeve 13. The inner wall of the groove is slidably connected to the disc 14, and the cross-section of the groove is circular.

[0025] Threaded holes are provided on the outer walls of the rectangular sleeve 13 and the circular ring 12, and bolts 15 are threadedly connected to the inner walls of the two threaded holes. One end of one bolt 15 is fixedly connected to the outer wall of the disc 14, and one end of the other bolt 15 is squeezed against the top of the top seat 9. The squeezing force between one end of the bolt 15 and the top seat 9 is relatively large. During centrifugation, the top seat 9 is fixed in position by the squeezing force and friction force of the bolt 15 and cannot rotate. A box cover 16 is hinged on the top of the separation placement box 1, and an electromechanical lock is installed between the box cover 16 and the separation placement box 1.

[0026] Working principle: when in use, the box cover 16 is opened through the electromechanical integrated lock to expose the interior of the separation and placement box 1, and multiple centrifuge tubes containing samples are placed in the multiple placement holes 10 respectively, and the bottom of the centrifuge tube is in contact with the top of the circular pad 6. Turning one of the bolts 15 drives the disc 14 to move outward, and the disc 14 no longer squeezes the outer wall of the rectangular rod 8, releasing the position fixation of the rectangular sleeve 13, and the rectangular sleeve 13, the circular ring 12 and the top seat 9 can move up and down through the rectangular rod 8 to adjust the limiting height of the centrifuge tube to facilitate the adjustment of the center of gravity position of the centrifuge tube; when limiting multiple centrifuge tubes, turning another bolt 15 releases the position fixation of the top seat 9, and the multiple placement holes 10 on the rotating top seat 9 are staggered with the multiple placement holes 10 on the round seat 7 to form a clamping effect for multiple centrifuge tubes, and turning another bolt 15 again fixes the top seat 9 to prevent it from rotating. The subsequent motor 3 drives the circular end seat 4 to rotate, forming a centrifugal separation effect.

[0027] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to specific embodiments. Obviously, numerous modifications and variations are possible based on the contents of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A biological cell separation device, comprising a separation and placement box (1), characterized in that: The bottom of the separation placement box (1) is fixedly connected to an equipment box (2), the interior of the equipment box (2) is fixedly connected to a motor (3), the top of the output shaft of the motor (3) is fixedly connected to a circular end seat (4), the top of the circular end seat (4) is fixedly connected to a circular pad (6), the top of the circular pad (6) is fixedly connected to a round seat (7), the top of the round seat (7) is provided with a placement mechanism, the placement mechanism includes a rectangular rod (8), the top of the round seat (7) is fixedly connected to the bottom of the rectangular rod (8), the outer wall of the rectangular rod (8) is provided with a top seat (9), the top seat (9) and the outer wall of the round seat (7) are evenly provided with a plurality of placement holes (10), the inner walls of the plurality of placement holes (10) are fixedly connected to a silicone ring (11), the top of the round seat (7) is rotatably connected to a circular ring (12), and the top of the round seat (7) is provided with a lifting mechanism.

2. A biological cell separation device according to claim 1, characterized in that: The lifting mechanism comprises a rectangular sleeve (13), the top of the circular ring (12) is fixedly connected to the bottom of the rectangular sleeve (13), the outer wall of the rectangular sleeve (13) is provided with a groove, the inner wall of the groove is slidably connected to a disc (14), the outer walls of the rectangular sleeve (13) and the circular ring (12) are both provided with threaded holes, the inner walls of the two threaded holes are both threadedly connected with bolts (15), one end of one of the bolts (15) is fixedly connected to the outer wall of the disc (14), and one end of the other bolt (15) is pressed against the top of the top seat (9).

3. The biological cell separation device according to claim 1, characterized in that: A through hole is provided at the bottom of the separation storage box (1), and the inner wall of the through hole is rotatably connected to the outer wall of the output shaft of the motor (3).

4. The biological cell separation device according to claim 1, characterized in that: The bottom of the circular end seat (4) is rotatably inlaid with a plurality of balls (5), and the outer walls of the plurality of balls (5) are in contact with the inner wall of the bottom of the separation placement box (1).

5. The biological cell separation device according to claim 2, characterized in that: The inner wall of the rectangular sleeve (13) is slidably connected to the outer wall of the rectangular rod (8), and the outer wall of the disc (14) is pressed against the outer wall of the rectangular rod (8).

6. The biological cell separation device according to claim 1, characterized in that: The top of the separation storage box (1) is hinged with a box cover (16), and an electromechanical integrated lock is installed between the box cover (16) and the separation storage box (1).

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