Centrifugal device for cell in-vitro separation

Abstract

The utility model relates to the field of cell separation, in particular to a centrifugal device for in-vitro separation of cells, and provides the following scheme aiming at the problems that a container of a cell centrifugal device in the prior art is inconvenient to take out and not convenient enough to fix, the centrifugal device comprises a machine body and a placing disc, and a mounting hole for mounting the placing disc is formed in the top of the machine body; the placing disc is located in the mounting hole, and the bottom of the placing disc is rotationally connected with the mounting hole; an adjusting hole and a plurality of placing holes are formed in the top face of the placing disc, the adjusting hole and the placing disc are coaxially arranged, the placing holes are all located on the outer side of the adjusting hole, a movable block is installed on the inner wall of the bottom of the adjusting hole through an elastic piece, and an air cylinder is installed on the top face of the movable block. According to the centrifugal machine, not only can a container be conveniently taken after the centrifugal machine is finished, but also the container can be fixed after being placed in the placing hole, so that the container is prevented from shaking in the centrifugal process.

Description

Technical Field

[0001] The utility model relates to the field of cell separation, in particular to a centrifugal device for in vitro cell separation. Background Art

[0002] The purpose of cell centrifugation is to separate different components in the culture medium. Currently, centrifugation is generally performed by placing the items to be centrifuged on a centrifuge rotor and then rotating them at high speed to achieve the purpose of centrifugation.

[0003] In order to ensure that the container containing cells remains stable during the rotation process, the container is usually fixed by a special mechanism when it is placed in the centrifuge, and then the fixing mechanism is opened after the centrifugation is completed, and then the container is taken out. During this process, the container needs to be fixed and loosened separately, and the placement hole specially provided on the centrifuge for placing the container is generally consistent with the shape of the container, so as to ensure that the container does not shake during the rotation process. However, this also leads to the need to laboriously take the container out of the placement hole when taking out the container, which brings certain inconveniences to the operator. For this reason, this scheme proposes a centrifugal device for in vitro cell separation. Utility Model Content

[0004] The utility model provides a centrifugal device for in vitro cell separation, which solves the problems of inconvenience in removing containers and inconvenience in fixing containers in the cell centrifugal device of the prior art.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A centrifugal device for in vitro cell separation comprises a body and a placement plate, wherein a mounting hole for mounting the placement plate is provided on the top of the body, the placement plate is located in the mounting hole, and the bottom of the placement plate is rotatably connected to the mounting hole;

[0007] The top surface of the placement plate is provided with an adjustment hole and a plurality of placement holes, the adjustment hole is coaxially arranged with the placement plate, and the plurality of placement holes are all located outside the adjustment hole, a movable block is installed on the inner wall of the bottom of the adjustment hole through an elastic member, an air cylinder is installed on the top surface of the movable block, a piston plate is installed in the air cylinder, a stud is threadedly connected to the top of the air cylinder, the bottom of the stud extends into the air cylinder and is rotatably connected to the top surface of the piston plate, and a positioning assembly is installed on the top of the stud;

[0008] A connecting groove is provided between the placement hole and the adjustment hole, a supporting plate is provided in the placement hole, a connecting plate is fixed to the outer periphery of the supporting plate, and the other end of the connecting plate passes through the connecting groove and is fixed to the outer periphery of the movable block, an inflatable rubber ring is installed on the inner ring of the placement hole, and the inflatable rubber ring is connected to the air cylinder through an air pipe;

[0009] A fixing mechanism for fixing the air cylinder is installed on the top surface of the placement plate.

[0010] The above technical solution not only makes it convenient to take out the container after the centrifugation is completed, but also can fix the container after it is placed in the placement hole, thereby preventing the container from shaking during the centrifugation process.

[0011] As a further improvement of the above scheme, the positioning assembly includes an adjustment plate fixed on the top of the stud and a positioning bolt threadedly connected to the top surface of the adjustment plate, the bottom of the positioning bolt extends to the bottom of the adjustment plate, and the top surface of the air cylinder is provided with a positioning hole that matches the positioning bolt.

[0012] Through the above technical solution, the stud can be conveniently locked to prevent it from loosening during the centrifugal process.

[0013] As a further improvement of the above solution, the number of the fixing mechanisms is at least two, and the fixing mechanisms include a fixing plate rotatably connected to the top surface of the placement plate and a fixing bolt threadedly connected to the top surface of the fixing plate, the bottom of the fixing bolt extends to below the fixing plate, and the top surface of the air cylinder is provided with a fixing hole that matches the fixing bolt.

[0014] Through the above technical solution, the fixed cylinder can be conveniently fixed, thereby preventing it from bouncing upward during the centrifugal process.

[0015] As a further improvement of the above solution, the number of the elastic members is at least two, and a plurality of receiving holes for installing the elastic members are provided at the bottom of the adjustment hole. The elastic member includes a spring fixed at the bottom of the receiving hole and a movable column movably arranged in the receiving hole. The bottom of the movable column is fixedly connected to the top of the spring, and the top of the movable column is fixedly connected to the bottom surface of the movable block.

[0016] Through the above technical solution, the movable block can automatically bounce upward after the pressure applied to the air cylinder is released.

[0017] As a further improvement of the above solution, the inner circle of the placement hole is provided with a mounting groove coaxially arranged therewith, the inflatable rubber ring is installed in the mounting groove, and the inflatable rubber ring is coaxially arranged with the placement hole.

[0018] Through the above technical solution, the inflatable rubber ring will retract into the installation groove before inflation, and can expand after inflation. Its inner ring will enter the placement hole and be tightly wrapped around the outer circumference of the container, thereby fixing the container.

[0019] As a further improvement of the above solution, an installation cavity is opened inside the body, and the installation cavity is located below the installation hole. A motor is installed in the installation cavity, and the output shaft of the motor is transmission-connected to the bottom surface of the placement plate.

[0020] Through the above technical solution, the placement tray is driven to rotate by the rotation of the motor.

[0021] As a further improvement of the above solution, a plurality of balls are installed on the bottom surface of the placement plate, and the bottom surfaces of the balls are rollingly connected to the bottom surface of the mounting hole.

[0022] Through the above technical solution, the balls are used to support the placement plate, thereby maintaining its stability during the rotation process.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] 1. Through the cooperation between the elastic member, the movable block, the fixing mechanism and the gas cylinder, when taking out the container placed in the placement hole, the fixing mechanism can be moved away from the top of the gas cylinder. Under the action of the elastic member, the movable block will automatically bounce upward, thereby driving the support plate to move up, thereby pushing the container placed in the placement hole upward, making it easier for the operator to take it out.

[0025] 2. Through the cooperation between the air cylinder, screw, positioning assembly and inflatable rubber ring, after the container is placed tightly inside the placement hole, the screw is rotated to drive the piston plate down, thereby pressing the air in the air cylinder into the inflatable rubber ring. After the inflatable rubber ring is inflated, it expands and clamps the container in the placement hole, preventing the container from shaking during the centrifugal process. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a three-dimensional diagram of the utility model;

[0027] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0028] Figure 3 It is a top view of the utility model;

[0029] Figure 4 It is a front sectional view of the present utility model.

[0030] Description of main symbols:

[0031] 1. Machine body; 2. Placement plate; 3. Placement hole; 4. Inflatable rubber ring; 5. Fixing plate; 6. Fixing bolt; 7. Adjustment plate; 8. Stud; 9. Connection groove; 10. Air cylinder; 11. Positioning hole; 12. Positioning bolt; 13. Mounting cavity; 14. Support plate; 15. Connecting plate; 16. Motor; 17. Movable block; 18. Ball bearing; 19. Fixing hole; 20. Storage hole; 21. Movable column; 22. Piston plate; 23. Air pipe. DETAILED DESCRIPTION

[0032] Below, the present invention is further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0033] Example 1:

[0034] Please combine Figure 1 - Figure 4 A centrifugal device for in vitro cell separation in this embodiment includes a body 1 and a placement tray 2. A mounting hole for mounting the placement tray 2 is provided on the top of the body 1. The placement tray 2 is located in the mounting hole, and the bottom of the placement tray 2 is rotatably connected to the mounting hole. A mounting cavity 13 is provided inside the body 1. The mounting cavity 13 is located below the mounting hole. A motor 16 is installed in the mounting cavity 13. The output shaft of the motor 16 is transmission-connected to the bottom surface of the placement tray 2. The rotation of the motor 16 drives the placement tray 2 to rotate, thereby centrifuging the cells placed on the placement tray 2.

[0035] An adjustment hole and multiple placement holes 3 are provided on the top surface of the placement plate 2. The adjustment hole is coaxially arranged with the placement plate 2. The multiple placement holes 3 are all located outside the adjustment hole, and the multiple placement holes 3 are distributed in a circular array with the adjustment hole as the center. During centrifugation, the container can be placed in the placement hole 3.

[0036] A movable block 17 is installed on the inner wall of the bottom of the adjustment hole through an elastic member, and a gas cylinder 10 is installed on the top surface of the movable block 17. A connecting groove 9 is provided between the placement hole 3 and the adjustment hole. A supporting plate 14 is provided in the placement hole 3. A connecting plate 15 is fixed to the outer periphery of the supporting plate 14, and the other end of the connecting plate 15 passes through the connecting groove 9 and is fixed to the outer periphery of the movable block 17. The number of elastic members is at least two, and a plurality of receiving holes 20 for installing elastic members are provided at the bottom of the adjustment hole. The elastic member includes a spring fixed to the bottom of the receiving hole 20 and an active spring movably provided in the receiving hole 20. The movable column 21 is fixed to the top of the spring, and the top of the movable column 21 is fixed to the bottom surface of the movable block 17. The top surface of the placement plate 2 is installed with a fixing mechanism for fixing the air cylinder 10. The number of the fixing mechanisms is at least two. The fixing mechanism includes a fixing plate 5 rotatably connected to the top surface of the placement plate 2 and a fixing bolt 6 threadedly connected to the top surface of the fixing plate 5. The bottom of the fixing bolt 6 extends below the fixing plate 5. The top surface of the air cylinder 10 is provided with a fixing hole 19 that matches the fixing bolt 6. When the fixing mechanism does not fix the air cylinder 10, the spring is in When the cylinder 10 is in a relaxed state, the cylinder 10 is located outside the adjustment hole, and the support plate 14 is also in an upward state. At this time, the container placed in the placement hole 3 falls on the top surface of the support plate 14, and half of the length of the container is located outside the placement hole, which makes it convenient for the operator to take the container. When the cylinder 10 is pressed down, the movable block 17 and the support plate 14 will drop accordingly, and the spring will also shrink accordingly. Until the movable block 17 touches the bottom of the adjustment hole, the container will completely fall into the placement hole 3. At this time, the cylinder 10 also completely falls into the adjustment hole, and then Then rotate the fixing plate 5 so that one end of it rotates to the top surface of the gas cylinder 10, and then screw the bottom of the fixing bolt 6 into one of the fixing holes 19, and then fix the other fixing mechanisms in the same way to fix the gas cylinder 10. In this way, the height of the supporting plate 14 and the movable block 17 will not change during the rotation of the placement tray 3. On the contrary, after the fixing plate 5 is removed from the top of the gas cylinder 10, the movable block 17 will bounce upward under the action of the spring, and then drive the supporting plate 14 to move upward, thereby pushing the container in the placement hole 3 upward.

[0037] A piston plate 22 is installed in the air cylinder 10, and a stud 8 is threadedly connected to the top of the air cylinder 10. The bottom of the stud 8 extends into the air cylinder 10 and is rotatably connected to the top surface of the piston plate 22. A positioning component is installed on the top of the stud 8. An inflatable rubber ring 4 is installed on the inner ring of the placement hole 3. The inner ring of the placement hole 3 is provided with a mounting groove coaxially arranged therewith. The inflatable rubber ring 4 is installed in the mounting groove, and the inflatable rubber ring 4 is coaxially arranged with the placement hole 3. The inflatable rubber ring 4 is connected to the air cylinder 10 through the air pipe 23. After the movable block 17 contacts the bottom of the adjustment hole, the screw is rotated clockwise. The stud 8 is rotated counterclockwise to drive the piston plate 22 downward. After the piston plate 22 descends, the air in the cylinder 10 is respectively input into the multiple inflatable rubber rings 4 through the multiple air pipes 23. The inflatable rubber rings 4 expand after being inflated, so that the inner ring of the inflatable rubber ring 4 enters the placement hole 3 and is tightly wrapped around the outer periphery of the container, so that the container will not shake during the centrifugal process. Conversely, when the stud 8 is rotated counterclockwise, the piston plate 22 can be driven to move upward, thereby drawing the air in the inflatable rubber ring 4 back into the cylinder 10, and the inflatable rubber ring 4 deflates, thereby loosening the tightly wrapped container.

[0038] The positioning assembly includes an adjustment plate 7 fixed to the top of the stud 8 and a positioning bolt 12 threadedly connected to the top surface of the adjustment plate 7. The bottom of the positioning bolt 12 extends to the bottom of the adjustment plate 7. The top surface of the air cylinder 10 is provided with a positioning hole 11 that matches the positioning bolt 12. After the bottom of the positioning bolt 12 is screwed into the positioning hole 11, the stud 8 cannot rotate, thereby preventing the stud 8 from rotating during the centrifugal process.

[0039] The implementation principle of this embodiment is as follows: during centrifugation, the container containing cells is first placed in the placement hole 3 on the placement plate 2 in sequence. After the container is placed, the air cylinder 10 is pressed down until the movable block 17 touches the bottom of the adjustment hole. Then, the fixing plate 5 is rotated so that the top surface of the air cylinder 10 rotates at one end. Then, the bottom of the fixing bolt 6 is screwed into one of the fixing holes 19. Then, the other fixing mechanisms are fixed in the same manner to fix the air cylinder 10. Then, the stud 8 is rotated clockwise to press the air in the air cylinder 10 into the interior of the multiple inflatable rubber rings 4, and then the container can be tightened. Then, the bottom of the positioning bolt 12 is screwed into the interior of the positioning hole 11 to lock the stud 8. Finally, the motor 16 can be started to drive the placement plate 2 to rotate, thereby centrifuging the cells.

[0040] After the centrifugal shaft is completed, first unscrew the positioning bolt 12 to the outside of the positioning hole 11, and then rotate the stud 8 counterclockwise to return it to its original position. When the inflated rubber ring 4 releases the container, open the fixing mechanism. When opening the fixing mechanism, first unscrew the fixing bolt 6 to the outside of the fixing hole 19, and then rotate the fixing plate 5 so that the fixing plate 5 deviates from the top of the air cylinder 10. After all the fixing mechanisms are opened, release the air cylinder 10. Under the action of the spring, the movable block 17 will bounce upward, and then drive the support plate 14 to move upward, thereby pushing the container in the placement hole 3 upward, making it easier for the operator to take the container.

[0041] Example 2:

[0042] Combine Figure 2 and Figure 4 The present embodiment is further improved on the basis of the embodiment 1 in that: a plurality of balls 18 are mounted on the bottom surface of the placement plate 2, and the bottom surface of the balls 18 is in rolling connection with the bottom surface of the mounting hole.

[0043] The implementation principle of this embodiment is: the setting of the ball 18 can not only drive the support plate 2, but also will not affect the rotation of the plate 2, and can also ensure that the plate 2 will be more stable during rotation.

[0044] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and replacements made by technicians in this field on the basis of the present invention fall within the scope of protection required by the present invention.

Claims

1. A centrifugal device for in vitro cell separation, comprising a body and a placement plate, characterized in that: The top of the body is provided with a mounting hole for mounting a placement tray, the placement tray is located in the mounting hole, and the bottom of the placement tray is rotatably connected to the mounting hole; The top surface of the placement plate is provided with an adjustment hole and a plurality of placement holes, the adjustment hole is coaxially arranged with the placement plate, and the plurality of placement holes are all located outside the adjustment hole, a movable block is installed on the inner wall of the bottom of the adjustment hole through an elastic member, an air cylinder is installed on the top surface of the movable block, a piston plate is installed in the air cylinder, a stud is threadedly connected to the top of the air cylinder, the bottom of the stud extends into the air cylinder and is rotatably connected to the top surface of the piston plate, and a positioning assembly is installed on the top of the stud; A connecting groove is provided between the placement hole and the adjustment hole, a supporting plate is provided in the placement hole, a connecting plate is fixed to the outer periphery of the supporting plate, and the other end of the connecting plate passes through the connecting groove and is fixed to the outer periphery of the movable block, an inflatable rubber ring is installed on the inner ring of the placement hole, and the inflatable rubber ring is connected to the air cylinder through an air pipe; A fixing mechanism for fixing the air cylinder is installed on the top surface of the placement plate.

2. A centrifugal device for in vitro cell separation according to claim 1, characterized in that: The positioning assembly includes an adjustment plate fixed on the top of the stud and a positioning bolt threadedly connected to the top surface of the adjustment plate. The bottom of the positioning bolt extends below the adjustment plate, and the top surface of the air cylinder is provided with a positioning hole that matches the positioning bolt.

3. A centrifugal device for in vitro cell separation according to claim 1, characterized in that: The number of the fixing mechanisms is at least two, and the fixing mechanisms include a fixing plate rotatably connected to the top surface of the placement tray and a fixing bolt threadedly connected to the top surface of the fixing plate. The bottom of the fixing bolt extends below the fixing plate, and the top surface of the air cylinder is provided with a fixing hole that matches the fixing bolt.

4. A centrifugal device for in vitro cell separation according to claim 1, characterized in that: The number of the elastic members is at least two, and a plurality of receiving holes for installing the elastic members are provided at the bottom of the adjustment hole. The elastic member includes a spring fixed at the bottom of the receiving hole and a movable column movably arranged in the receiving hole. The bottom of the movable column is fixedly connected to the top of the spring, and the top of the movable column is fixedly connected to the bottom surface of the movable block.

5. The centrifugal device for in vitro cell separation according to claim 1, characterized in that: The inner circle of the placement hole is provided with a mounting groove coaxially arranged therewith, the inflatable rubber ring is installed in the mounting groove, and the inflatable rubber ring is coaxially arranged with the placement hole.

6. The centrifugal device for in vitro cell separation according to claim 1, characterized in that: An installation cavity is provided inside the machine body and is located below the installation hole. A motor is installed in the installation cavity, and an output shaft of the motor is transmission-connected to the bottom surface of the placement plate.

7. The centrifugal device for in vitro cell separation according to claim 1, characterized in that: A plurality of balls are installed on the bottom surface of the placement plate, and the bottom surfaces of the balls are rollingly connected to the bottom surface of the installation hole.

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