Solid passive sampler suitable for eDNA in natural water body

Abstract

The utility model discloses an eDNA solid state passive sampler suitable for natural water, and relates to the eDNA sampler field, the eDNA solid state passive sampler comprises a lure pole, a lure wheel and a main line, the lure wheel is fixedly arranged on a wheel seat of the lure pole, one end of the main line is wound on a line cup of the lure wheel, the other end of the main line passes through a line guide ring arranged on the lure pole, and the line guide ring is arranged on the lure pole. The device further comprises a buoy, a buoy fixing hook, a slidable buoy seat, a stainless steel nesting device and an adsorption material filter membrane. According to the solid passive sampler suitable for the eDNA in the natural water body, the design that a stainless steel nesting device and a lure rod are combined is adopted, so that the sampling process is simple and rapid, complex equipment is not needed, and rapid deployment and recovery in a field environment are facilitated; the sampler can be kept stable under different water flow conditions, the problem that the sampler is lost along with water or washed away is effectively prevented, and the sampling efficiency and the success rate are greatly improved.

Description

Technical Field

[0001] The utility model relates to an eDNA sampler technology, in particular to an eDNA solid-state passive sampler suitable for natural water bodies. Background Art

[0002] In the fields of environmental science, ecology, and aquatic biology, environmental DNA (eDNA) analysis has become an important monitoring method for assessing biodiversity, tracking alien species, monitoring pathogens, and studying ecosystem health. Environmental DNA refers to DNA fragments that remain in the water bodies of aquatic organisms through feces, mucus, skin shedding, and corpse degradation. After collecting environmental samples and extracting DNA, conserved primers targeting single gene regions in multiple species can be used for amplification, and then data on their presence and relative abundance in the sample can be obtained. This method does not require the use of tissues or organisms of the target species and can detect target organisms non-invasively.

[0003] Existing eDNA enrichment methods often rely on active filtration, which not only requires complex equipment but is also difficult and inefficient to operate in the field, particularly in environments with high flow or deep water. Passive sampling methods, such as glass fiber filters, while simple to operate, are prone to failure in areas with rapid currents or deep water, making them ineffective in capturing eDNA. Therefore, developing a passive sampler that is both suitable for complex field environments and can efficiently and stably enrich eDNA is crucial. Utility Model Content

[0004] The purpose of the utility model is to provide a solid-state passive eDNA sampler suitable for natural water bodies to solve the above-mentioned deficiencies in the prior art.

[0005] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: a solid-state passive sampler for eDNA in natural water bodies, comprising a lure rod, a lure reel and a main line, the lure reel being fixedly mounted on the reel seat of the lure rod, one end of the main line being wound around the spool of the lure reel, and the other end of the main line passing through the wire ring provided on the lure rod, and also comprising: a float, a fixed float hook, a slidable float seat, a stainless steel nest maker and an adsorption material filter membrane, the stainless steel nest maker being fixedly mounted on the end of the main line away from the lure reel, the adsorption material filter membrane being installed in the stainless steel nest maker, the slidable float seat being passed through the main line and being located at the end of the main line close to the stainless steel nest maker, the float being installed on the slidable float seat, one end of the fixed float hook being sleeved on the bottom end of the float, and the other end of the fixed float hook being sleeved on the outside of the main line.

[0006] Furthermore, the lure rod adopts a straight-handled rod, and the lure reel adopts a spinning reel specially designed for long-distance casting.

[0007] Furthermore, the lure rod adopts a gun-handle rod, and the lure reel adopts a long-distance casting special water drop reel.

[0008] Furthermore, the main line is fishing line No. 1, with a diameter of 0.125mm to 0.165mm.

[0009] Furthermore, the float is a thick-tailed and large-headed float weighing 12g.

[0010] Furthermore, the material of the slidable float seat is selected from silicone.

[0011] Furthermore, the stainless steel nest maker adopts a stainless steel nest cage with an outer diameter of 7 cm.

[0012] Furthermore, the diameter of the adsorption material filter membrane is 5 cm and can be installed in a stainless steel nest maker.

[0013] Compared with the existing technology, the present invention provides a solid-state passive eDNA sampler suitable for natural water bodies. It adopts a design that combines a stainless steel nesting device with a lure rod, making the sampling process simple and quick. It does not require complex equipment and is easy to deploy and recover quickly in the field environment. The coordinated effect of the float, fixed float hook and sliding float seat ensures that the sampler can remain stable under different water flow conditions, effectively preventing the sampler from being lost or washed away with the water, and greatly improving the sampling efficiency and success rate.

[0014] By installing the adsorption material filter membrane in a stainless steel nesting device, sampling is convenient and easy to disassemble and assemble, which is beneficial to field operations. For eDNA sampling in natural water bodies, replacing different adsorption material filter membranes reduces the complexity and cost of sampling. It can fully and efficiently adsorb and enrich eDNA in water bodies, providing high-quality samples for subsequent molecular biology analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0016] Figure 1 This is a schematic diagram of the overall structure provided by an embodiment of the present utility model.

[0017] Description of reference numerals:

[0018] 1. Lure reel; 2. Lure rod; 3. Main line; 4. Stainless steel nesting device; 5. Adsorption material filter membrane; 6. Sliding float seat; 7. Fixed float hook; 8. Float. DETAILED DESCRIPTION

[0019] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0020] See also Figure 1 , a solid-state passive sampler for eDNA in natural water bodies, comprising a lure rod 2, a lure reel 1 and a main line 3, the lure reel 1 is fixedly mounted on the reel seat of the lure rod 2, one end of the main line 3 is wound around the spool of the lure reel 1, and the other end of the main line 3 passes through the wire ring provided on the lure rod 2, and also includes: a float 8, a fixed float hook 7, a slidable float seat 6, a stainless steel nesting device 4 and an adsorption material filter membrane 5, the stainless steel nesting device 4 is fixedly mounted on the end of the main line 3 away from the lure reel 1, the adsorption material filter membrane 5 is installed in the stainless steel nesting device 4, the slidable float seat 6 is passed through the main line 3 and is located at the end of the main line 3 close to the stainless steel nesting device 4, the float 8 is mounted on the slidable float seat 6, one end of the fixed float hook 7 is sleeved on the bottom end of the float 8, and the other end of the fixed float hook 7 is sleeved on the outside of the main line 3.

[0021] eDNA currently mainly uses active sampling methods, which have many drawbacks. During the collection, transportation, storage, and filtration of water samples, eDNA will degrade, affecting the accuracy of the analysis. Passive sampling methods for eDNA in water have only been studied in the past two years. Passive sampling methods are not affected by water quality and are equally effective in turbid water bodies. Compared with active sampling, passive sampling methods do not rely on filtering instruments and power supplies, greatly reducing the time and consumable costs of field sampling, minimizing cross-contamination between different samples, and enabling effective comparisons between eDNA studies conducted at different times and locations. Long-term and effective ecological monitoring data can be obtained, allowing for more efficient and accurate research on aquatic biodiversity and the distribution of pathogens. However, in natural water bodies with excessive shore weeds and poor access to flowing water, existing technologies are unable to complete sampling work.

[0022] Existing eDNA enrichment methods mostly rely on active filtration, which not only requires complex equipment but is also difficult and inefficient to operate in the field, especially in environments with high mobility or deep water. Passive sampling methods, such as using glass fiber filters, while simple to operate, are prone to failure in rapids or deep waters, and are easily swept away by the water, making it difficult to effectively capture eDNA.

[0023] To this end, the present application connects a stainless steel nest maker 4 equipped with an adsorption material filter membrane 5 to the end of a main line 3, adjusts the length of the main line 3 through a lure reel 1, and sets a float 8, a fixed float hook 7, and a slidable float seat 6 on the main line 3. Utilizing the casting function of the lure rod 2, the stainless steel nest maker 4 and the adsorption material filter membrane 5 are accurately dropped into the target water body. The float 8 serves to ensure that the stainless steel nest maker 4 equipped with the adsorption material filter membrane 5 does not sink to the bottom of the water, while the fixed float hook 7 and the slidable float seat 6 jointly ensure the stability of the stainless steel nest maker 4 equipped with the adsorption material filter membrane 5 under complex water flow conditions, ensuring that the stainless steel nest maker 4 equipped with the adsorption material filter membrane 5 has a good stability effect after entering the water regardless of the size of the wind and waves and the water flow.

[0024] After the stainless steel nest maker 4 enters the water, the adsorption material filter membrane 5 inside the stainless steel nest maker 4 starts to work, and uses its unique adsorption properties to efficiently capture and enrich eDNA in the water body. It is suitable for the collection of eDNA in natural water bodies, lakes and river basins, including but not limited to the rapid adsorption and detection of aquatic organisms, water microorganisms and pathogens. It can avoid concentrating water samples to obtain eDNA, and is not affected by water turbidity and highly fluid water bodies, thereby improving applicability.

[0025] After sampling is complete, the main line 3 is retrieved via the lure reel 1, and the stainless steel beeper 4 is pulled back from the water. The adsorbent filter membrane 5 is removed from the beeper 4 and immediately placed in a sterile, sealed bag for cryopreservation to maintain the integrity and stability of the eDNA for subsequent experimental analysis. This significantly reduces field sampling time and consumable costs, minimizes cross-contamination between samples, and enables effective comparison of eDNA studies conducted at different times and locations, providing long-term, effective ecological monitoring data for more efficient and accurate research on aquatic biodiversity and the distribution of pathogens.

[0026] The design of combining a stainless steel nest maker 4 with a lure rod 2 makes the sampling process simple and quick, without the need for complex equipment, and facilitates rapid deployment and recovery in the field environment. Through the coordinated action of the float 8, the fixed float hook 7 and the sliding float seat 6, the sampler can remain stable under different water flow conditions, effectively preventing the sampler from being lost or washed away with the water, and greatly improving the sampling efficiency and success rate.

[0027] In one embodiment of the present invention, the lure rod 2 adopts a straight-handled rod, and the lure reel 1 adopts a spinning reel specially designed for long-distance casting. The specific model can be selected according to needs.

[0028] In one embodiment of the present invention, the lure rod 2 adopts a gun-handle rod, and the lure reel 1 adopts a long-distance casting special water drop reel. The specific model can be selected according to needs.

[0029] In one embodiment of the present invention, the main line 3 is fishing line No. 1, with a diameter of 0.125 mm to 0.165 mm.

[0030] In one embodiment of the present invention, the fixed float hook 7 is of large size, suitable for 3-12 gram floats, and can be selected from one of the models including 1c, 2c, 3c, etc.

[0031] In one embodiment of the present invention, the float 8 is a thick-tailed and large-headed float weighing 12g.

[0032] In one embodiment of the present invention, the material of the sliding float seat 6 is selected from silicone material, and the model is large, which is suitable for a float 8 with a float foot diameter between 1.2 and 1.5 mm. Space beans or other fixing devices can also be used to fix the sliding float seat 6 on the main line 3 to prevent the sliding float seat 6 from moving at will.

[0033] In one embodiment of the present invention, the stainless steel nest maker 4 adopts a stainless steel nest cage, which is spherical and has an outer diameter of 7 cm. The stainless steel nest cage is provided with mesh holes. The circular structure and mesh hole design of the stainless steel nest cage help to achieve all-round and dead-angle eDNA collection, while effectively filtering plants and other impurities in the water.

[0034] In one embodiment of the present invention, the diameter of the adsorption material filter membrane 5 is 5 cm, which can be installed in the stainless steel nesting device 4. It is made of stainless steel, which is strong and durable and not easy to damage. At the same time, the adsorption material filter membrane 5 can be easily replaced, realizing the sustainable use of the sampler.

[0035] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A solid-state passive sampler for eDNA in natural water bodies, comprising a lure rod (2), a lure reel (1) and a main line (3), wherein the lure reel (1) is fixedly mounted on a reel seat of the lure rod (2), one end of the main line (3) is wound around a spool of the lure reel (1), and the other end of the main line (3) passes through a wire ring provided on the lure rod (2), characterized in that: Also includes: A float (8), a fixed float hook (7), a slidable float seat (6), a stainless steel nest maker (4) and an adsorption material filter membrane (5), wherein the stainless steel nest maker (4) is fixedly mounted on an end of a main line (3) away from a lure wheel (1), the adsorption material filter membrane (5) is mounted in the stainless steel nest maker (4), the slidable float seat (6) is passed through the main line (3) and is located at an end of the main line (3) close to the stainless steel nest maker (4), the float (8) is mounted on the slidable float seat (6), one end of the fixed float hook (7) is sleeved on the bottom end of the float (8), and the other end of the fixed float hook (7) is sleeved on the outside of the main line (3).

2. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The lure fishing rod (2) adopts a straight-handled rod, and the lure fishing reel (1) adopts a long-distance casting special spinning reel.

3. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The lure fishing rod (2) adopts a gun-handle rod, and the lure fishing reel (1) adopts a long-distance casting special water drop reel.

4. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The main line (3) is a No. 1 fishing line with a diameter of 0.125 mm to 0.165 mm.

5. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The float (8) is a thick tail and large head float with a weight of 12g.

6. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The material of the slidable float seat (6) is selected from silica gel.

7. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The stainless steel nest maker (4) adopts a stainless steel nest maker cage with an outer diameter of 7 cm.

8. The solid-state passive eDNA sampler for natural water bodies according to claim 1, characterized in that: The diameter of the adsorption material filter membrane (5) is 5 cm and can be installed in the stainless steel nesting device (4).

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